Search

Your search keyword '"Monleon Pradas, M."' showing total 39 results
Start Over Author "Monleon Pradas, M."
39 results on '"Monleon Pradas, M."'

5. A Strategy for Magnetic and Electric Stimulation to Enhance Proliferation and Differentiation of NPCs Seeded over PLA Electrospun Membranes

Author

Cuenca-Ortolá I, Martínez-Rojas B, Moreno Manzano V, García Castelló M, Monleon Pradas M, Martínez-Ramos C, and Más Estellés J

Abstract

Neural progenitor cells (NPCs) have been shown to serve as an efficient therapeutic strategy in different cell therapy approaches, including spinal cord injury treatment. Despite the reported beneficial effects of NPC transplantation, the low survival and differentiation rates constrain important limitations. Herein, a new methodology has been developed to overcome both limitations by applying a combination of wireless electrical and magnetic stimulation to NPCs seeded on aligned poly(lactic acid) nanofibrous scaffolds for in vitro cell conditioning prior transplantation. Two stimulation patterns were tested and compared, continuous (long stimulus applied once a day) and intermittent (short stimulus applied three times a day). The results show that applied continuous stimulation promotes NPC proliferation and preferential differentiation into oligodendrocytic and neuronal lineages. A neural-like phenotypic induction was observed when compared to unstimulated NPCs. In contrast, intermittent stimulation patterns did not affect NPC proliferation and differentiation to oligodendrocytes or astrocytes morphology with a detrimental effect on neuronal differentiation. This study provides a new approach of using a combination of electric and magnetic stimulation to induce proliferation and further neuronal differentiation, which would improve therapy outcomes in disorders such as spinal cord injury.

Published
2023

15. Elastomeric cardiopatch scaffold for myocardial repair and ventricular support

Author

Chachques JC, Lila N, Soler-Botija C, Martinez-Ramos C, Valles A, Autret G, Perier MC, Mirochnik N, Monleon-Pradas M, Bayes-Genis A, and Semino CE

Subjects
Cardiac tissue engineering, Cardiopatch, Cardiowrap, Elastomeric scaffold, Heart failure, Translational and clinical research
Abstract

Prevention of postischaemic ventricular dilatation progressing towards pathological remodelling is necessary to decrease ventricular wall deterioration. Myocardial tissue engineering may play a therapeutic role due to its capacity to replace the extracellular matrix, thereby creating niches for cell homing. In this experimental animal study, a biomimetic cardiopatch was created with elastomeric scaffolds and nanotechnologies.

Published
2020

28. Physical ageing of amorphous polymers. Theroretical analysis and experiments on poly(methyl methacrylate)

Author

Perez, J., jean-yves CAVAILLE, Diaz Calleja, R., Gomez Ribelles, J. L., Monleon Pradas, M., Ribes Greus, A., Centre de Recherches sur les Macromolécules Végétales (CERMAV), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Joseph Fourier - Grenoble 1 (UJF), and Carret, Michèle

Subjects
ComputingMilieux_MISCELLANEOUS
Abstract

International audience

Published
1991

39. Future design of a new keratoprosthesis. Physical and biological analysis of polymeric substrates for epithelial cell growth.

Author

Campillo-Fernandez AJ, Pastor S, Abad-Collado M, Bataille L, Gomez-Ribelles JL, Meseguer-Dueñas JM, Monleon-Pradas M, Artola A, Alio JL, and Ruiz-Moreno JM

Subjects
Acrylates chemistry, Biocompatible Materials chemistry, Cell Adhesion, Cell Differentiation, Cell Line, Cell Proliferation, Conjunctiva cytology, Epithelial Cells cytology, Epithelial Cells drug effects, Epithelial Cells physiology, Humans, Methacrylates chemistry, Polymers chemistry, Polymers pharmacology, Acrylates pharmacology, Biocompatible Materials pharmacology, Cell Culture Techniques methods, Conjunctiva drug effects, Conjunctiva growth & development, Methacrylates pharmacology, Prostheses and Implants
Abstract

One of the main issues in the development of new biocolonizable materials is to understand the influence of the synthetic material on the biological response in terms of cellular adhesion, proliferation, and differentiation. In this study, we characterized different polymeric materials (with different hydrophobicity/hydrophilicity ratios and electrical charges) using dynamic-mechanical analysis, equilibrium water content, and surface energy. Cell adhesion, viability, morphology, and proliferation studies were conducted with these materials using a conjunctival epithelial cell line (IOBA-NHC). The biological data regarding physicochemical parameters of the materials were also correlated. When conjunctival epithelial cells were grown on poly(ethyl acrylate-co-hydroxyethyl acrylate) copolymers, P(EA-co-HEA), samples with up to 20% hydrophilic groups on their polymeric chain showed adhesion, viability, and proliferation, although these three factors decreased as the hydrophilic group content increased. The poly(ethyl acrylate-co-methacrylic acid) 90/10 copolymer, P(EA-co-MAAc) 90/10, showed better results than poly(ethyl acrylate-co-hydroxyethyl acrylate) copolymers and were even better than tissue control polystyrene (TCPS). This feature is explained by the presence of electrical charges on the surface of the poly(ethyl acrylate-co-methacrylic acid) 90/10 copolymer. The fact that the ionic groups are configured in domains structured in nanophases as happens in this copolymer improves cell adhesion even further.

Published
2007
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results