354 results on '"Moncef Mokni"'
Search Results
2. microRNAs expression profile in phyllodes tumors of the breast
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Mohamed Ridha Hachana, Mouna Maatouk, Zayneb Lassouad, Badreddine Sriha, and Moncef Mokni
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Phyllodes tumor ,MiroRNA ,Fibroadenoma ,Upregulation ,Downregulation ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Proliferation of both stromal and epithelial components is a characteristic of fibroepithelial cancers of the breast. Certain fibroepithelial tumors of the breast, such as fibradenomas and phyllodes tumors, are challenging to distinguish and categorize. To find biomarkers for early diagnosis and improved disease management, it is crucial to deepen our understanding of the molecular pathogenesis pathways and tumor biology of PTs. It has been demonstrated that microRNAs (miRNAs) have significant roles in cancers; the expression pattern of miRNAs can help with cancer categorization and treatment. In contrast, little is understood about miRNAs in breast fibroepithelial cancers.This study was conducted retrospectively with the goal of assessing the expression of six mature miRNAs (hsa-miR-21, hsa-miR-155, hsa-miR-182, hsa-miR-34a, hsa-miR-148a, and hsa-miR-205) in breast fibroepithelial cancers using real-time PCR and predicting these miRNAs' targets using computational techniques.This study comprised 64 patients in total—55 with phyllodes tumors and 9 with fibroadenoma. The research was carried out at the Farhat Hached University Hospital's pathology department in Tunisia. These particular miRNAs expression levels were evaluated via qRT-PCR, and in silico techniques were utilized to predict potential miRNA targets.Analysis of miRNA expression in fibroadenoma and phyllodes tumor tissues revealed that miR-21, miR-155 and miR-182 were upregulated in PTs compared to fibroadenoma and normal tissues. We reported that miR-34a, miR-148a and miR-205 were downregulated in both borderline and malignant PTs compared to fibroadenoma and normal tissue. In silico miRNA target prediction suggested the involvement of these molecules in a wide context of cell signaling pathways.
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- 2024
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3. A case of primary mesenteric synovial sarcoma: a challenging presentation
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Nihed Abdessayed, Malek Barka, Samiha Mabrouk, Zeineb Nfikha, Zeineb Maatoug, Yosra Fejji, Mohamed Salah Jarrar, Sabri Youssef, and Moncef Mokni
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Synovial ,Sarcoma ,Mesenteric ,Intra-abdominal ,Primary ,Surgery ,RD1-811 - Abstract
Abstract Background Synovial sarcoma is an uncommon soft tissue malignancy that mainly occurs near tendon sheath and bone joints. Primary intra-abdominal location is exceedingly rare and characterized by non-specific clinical signs. Case presentation We report the case of a young female without medical history who presented with acute abdominopelvic pain. Ultrasound echography revealed a right mass measuring 7 cm in greater diameter cystic with solid areas, likely of ovarian origin. A coelioscopy with peritoneal biopsies was performed. Histological examination with immunohistochemistry concluded the diagnosis of GIST. The patient was referred to the surgery department and after laboratory routine analysis and computed tomography, the patient was proposed to surgical management. Per-operative findings revealed a mesenteric mass locally invading the greater omentum and the appendicular wall. Pathological examination with immunochemistry confirmed the diagnosis of mesenteric monophasic synovial sarcoma invading the appendicular wall with positive surgical margins. Chemotherapy was proposed with a good response. Our patient is free from disease 9 months later. Conclusions We aimed through this case report to discuss mesenteric presentation monophasic SS, mimicking ovarian malignancy, emphasizing clinicopathological features and differential diagnoses.
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- 2023
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4. Death by late presenting of diaphragmatic hernia in an infant: case report and review of the literature
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Sarra Ben Abderrahim, Maher Jedidi, Amal Ben Daly, Zeineb Nfikha, Mohamed Ben Dhiab, Majed Zemni, Moncef Mokni, and Mohamed Kamel Souguir
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Congenital diaphragmatic hernia ,Case report ,Infant ,Sudden death ,Autopsy ,Law in general. Comparative and uniform law. Jurisprudence ,K1-7720 ,Medicine (General) ,R5-920 - Abstract
Abstract Background Congenital diaphragmatic hernia (CDH) is a congenital malformation of the diaphragm, resulting in the herniation of the abdominal organs into the thoracic cavity. If not properly diagnosed before or at birth, CDH represents a life-threatening pathology in infants and a major cause of death. We present a fatal case of congenital diaphragmatic hernia corresponding to Bochdalek hernia, discovered incidentally during a sudden death’s autopsy of an infant. To achieve a better view of the range of these anomalies, we also conducted a literature review on this subject describing the pathogenesis, manifestations, diagnosis, and autopsy contribution to addressing these CDH. Case presentation The case involved a 4-month-old female infant who presented sudden respiratory difficulties after breastfeeding. External examination found marked cyanosis with no evidence of trauma. Upon opening the chest cavity, the stomach, markedly distended, was occupying much of the left pleural cavity. The left lung was compressed and displaced superiorly, and the heart was also compressed and deviated to the right. This mediastinal deviation was due to an ascension of the stomach into the chest cavity through a 2 × 1.5 cm defect in the posterior left hemidiaphragm. Further examination remarked an ecchymotic appearance of the stomach portion entrapped in the hernia defect suggesting recent strangulation of the stomach. The lungs showed atelectasis with signs of pulmonary infection in the histology study. Conclusions CDH might be considered uncommon and not always mentioned in the list of sudden death in infant causes. Forensic pathologists should know of this malformation in order to apply the best autopsy techniques and thus allow positive feedback to pediatricians considering the possible legal implications.
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- 2022
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5. Phytochemical profiling, antioxidant potential and protective effect of leaves extract of tunisian Vitis vinifera autochthonous accessions against acute CCl4-injured hepatotoxicity in mice
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Nabil Saadaoui, Ahmed Mathlouthi, Ali Zaiter, Safia El-Bok, Moncef Mokni, Mounira Harbi, Néziha Ghanem-Boughanmi, Amadou Dicko, and Mossadok Ben-Attia
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Vitis vinifera L. ,Wild ,Cultivated ,Antioxidant activity ,Hepatoprotective activity ,Acute liver injury ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Vitis vinifera leaves (VVL) are agro-industrial waste. In the current study, the phytochemical profile of V. vinifera leaves extracts (VVLE) of two Tunisian autochthonous accessions was determined via LC-UV-ESI/MS, and their antioxidant and hepatoprotective properties were also assessed. Mice were pretreated orally with VVLE (7.5, 15 and 30 mg/kg) for 7 days, and then received acutely and by i.p. a solution CCl4 at 12% in sunflower oil (v/v). Serum levels of hepatic markers, oxidative stress indicators in liver tissue and histological changes were assessed. LC-UV-ESI/MS analysis revealed four phenolic compounds identified in both extracts with quercetin-3-O-glucuronide being the dominant constituent (23.32 ± 1.06 vs. 10.24 ± 0.12 mg/g DM, p
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- 2023
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6. Imported malaria in adults: about a case of cerebral malaria
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Sarra Ben Abderrahim, Sarra Gharsallaoui, Amal Ben Daly, Amal Mosbahi, Selma Chaieb, Zeineb Nfikha, Samar Ismaïl, Chahnez Makni, Moncef Mokni, Akila Fathallah-Mili, Maher Jedidi, and Mohamed Ben Dhiab
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Malaria infection ,Cerebral malaria ,Forensic medicine ,Autopsy ,Postmortem ,Tunisia ,Law in general. Comparative and uniform law. Jurisprudence ,K1-7720 ,Medicine (General) ,R5-920 - Abstract
Abstract Background Malaria is the first parasitic infection endemic in the world caused by parasites species of Plasmodium. Cerebral malaria (CM) is a rapidly progressive and severe form of Plasmodium falciparum infection, characterized by a greater accumulation of red blood cells parasitized by Plasmodium falciparum in the brain. The diagnosis of malaria is usually made in living patients from a blood sample taken in the course of a fever on return from an endemic country, whereas CM, often associated with fatal outcomes even in treated subjects, is usually diagnosed at autopsy. Case presentation We present the case of a 36-year-old man who died a few days after returning from a business trip to the Ivory Coast. As a result of an unclear cause of death, a medicolegal autopsy was ordered. Autopsy findings revealed massive congestion and edema of the brain with no other macroscopic abnormalities at organ gross examination. Histology and laboratory tests were conducted revealing a Plasmodium falciparum infection, with numerous parasitized erythrocytes containing dots of hemozoin pigment (malaria pigment) in all examined brain sections and all other organs. Death was attributed to cerebral malaria with multiple organ failure. Conclusions This report summarizes several features for the diagnosis of malaria and how postmortem investigations, as well as histology and laboratory diagnosis, may lead to a retrospective diagnosis of a fatal complicated form with cerebral involvement.
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- 2022
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7. Sarcoid-Like Reaction in the Kidney Following Rituximab for Mantle Lymphoma in a 60-Year-Old Man
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Sanda Mrabet, Rihem Dahmene, Asma Fradi, Achraf Jaziri, Raja Boukadida, Awatef Azzebi, Wissal Sahtout, Narjess Ben Aicha, Dorsaf Zellama, Abdellatif Achour, Nihed Abdessayed, and Moncef Mokni
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Medicine - Abstract
The sarcoid-like reaction is a rare autoinflammatory disease that can affect lymph nodes or organs but does not meet the diagnostic criteria for systemic sarcoidosis. Several drug classes have been associated with the development of a systemic sarcoid-like reaction, which defines drug-induced sarcoidosis-like reactions and can affect a single organ. Anti-CD20 antibodies (rituximab) have rarely been reported as responsible for this reaction and this adverse effect has mainly been described during the treatment of Hodgkin’s lymphoma. We report a unique case of a sarcoid-like reaction complicating rituximab following the treatment of a mantle cell lymphoma and interesting only the kidney. The 60-year-old patient presented with severe acute renal failure 6 months after the end of his r-CHOP protocol and the urgent renal biopsy revealed acute interstitial nephritis rich in granulomas without caseous necrosis. After ruling out other causes of granulomatous nephritis, a sarcoid-like reaction was retained since infiltration was limited to the kidney. The temporal relationship between rituximab administration and the sarcoid-like reaction onset in our patient supported the diagnosis of a rituximab-induced sarcoidosis-like reaction. Oral corticosteroid treatment led to rapid and lasting improvement in renal function. Clinicians should be warned of this adverse effect and regular and prolonged monitoring of renal function should be recommended during the follow-up of patients after the end of treatment with rituximab.
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- 2023
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8. An unexpected sudden death due to a choroid plexus papilloma: an autopsy case report
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Mohamed Amine Zaara, Sarra Gharsallaoui, Nihed Abdessayed, Sarra Mestiri, Dorra Chiba, Mohamed Ben Dhiab, and Moncef Mokni
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Choroid plexus ,Papilloma ,Sudden death ,Autopsy ,Case report ,Law in general. Comparative and uniform law. Jurisprudence ,K1-7720 ,Medicine (General) ,R5-920 - Abstract
Abstract Background Choroid plexus papillomas (CPPs) are histopathologically benign and rare central nervous system tumors. These tumors remain more frequent in children than adults. It is infrequent for these tumors to cause a sudden unexpected death. We aim in this case to discuss the unusual and fatal presentation of choroid plexus papilloma and the mechanism of death. Case presentation we report the case of a 61-year-old man with no medical history, diagnosed at autopsy with a previously unknown CPP. Initial complaints were chronic headache occurring in the last month and acute chest pain for two days. The forensic autopsy including the histopathologic examination showed a tumoral mass of the choroid plexus in the fourth ventricle diagnosed as a psammomatous CPP. The cause of death in this case was attributed to a massive cerebral edema caused by the tumoral mass. Conclusions Through this case report, we stress the importance of an early and a vigorous investigation of every headache and an early detection of this tumor and we highlight as well the role of the post mortem examination to detect such a fatal complication.
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- 2021
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9. Severe Acute Interstitial Nephritis, Dermatitis, and Hemolytic Anemia due to Polyparasitic Infection in an Immunocompetent Male Patient
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Sanda Mrabet, Wiem Romdhane, Asma Fradi, Raja Boukadida, Awatef Azzabi, Yosra Guedri, Wissal Sahtout, Narjess BenAicha, Nihed Abdessayed, Moncef Mokni, Dorsaf Zellama, and Abdellatif Achour
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Medicine - Abstract
Acute interstitial nephritis (AIN) is a relevant cause of acute renal failure. Drugs are the predominant cause, followed by infections and idiopathic lesions. AIN, as a form of hypersensitivity reaction, is an uncommon manifestation in the setting of human parasitic infections. We report a case of a polyparasitic infection ( Giardia lamblia, Entamoeba coli , and Endolimax nana ) resulting in a severe biopsy-proven AIN in a 61-year-old male patient. Despite the antiparasitic treatment followed by corticosteroid therapy, and during the 6-month follow-up period, the patient remained dialysis-dependent, and he developed autoimmune hemolytic anemia. Extensive search for another infection or neoplasia was negative. Immunological tests were also negative. The resulting hypersensitivity reaction to the triple parasite infection would have led to fatal evolution for the kidneys affected by this unusual type of AIN.
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- 2022
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10. Breast cancer incidence and predictions (Monastir, Tunisia: 2002-2030): A registry-based study.
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Imen Zemni, Meriem Kacem, Wafa Dhouib, Cyrine Bennasrallah, Rim Hadhri, Hela Abroug, Manel Ben Fredj, Moncef Mokni, Ines Bouanene, and Asma Sriha Belguith
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Medicine ,Science - Abstract
IntroductionBreast cancer is a major public health problem worldwide. It is the leading cause of cancer deaths in females. In developing countries like Tunisia, the frequency of this cancer is still growing. The aim of this study was to determine the crude and standardized incidence rates, trends and predictions until 2030 of breast cancer incidence rates in a Tunisian governorate.MethodsThis is a descriptive study including all female patients diagnosed with breast cancer in Monastir between 2002 and 2013. The data were collected from the cancer register of the center. Tumors were coded according to the 10th version of international classification of disease (ICD-10). Trends and predictions until 2030 were calculated using Poisson linear regression.ResultsA total of 1028 cases of female breast cancer were recorded. The median age of patients was 49 years (IQR: 41-59 years) with a minimum of 16 years and a maximum of 93 years. The age-standardized incidence rate (ASR) was of 39.12 per 100000 inhabitants. It increased significantly between 2002 and 2013 with APC of 8.4% (95% CI: 4.9; 11.9). Prediction until 2030 showed that ASR would reach 108.77 (95% CI: 57.13-209.10) per 100000 inhabitants.ConclusionThe incidence and the chronological trends of breast cancer highlighted that this disease is of a serious concern in Tunisia. Strengthening preventive measures is a primary step to restrain its burden.
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- 2022
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11. Significance of p53, p27, Ki-67, E-cadherin, and HER2 expression in upper urinary tract urothelial carcinoma
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Nabiha Missaoui, Ahlem Bdioui, Atika Baccouche, Oussema Belkacem, Wissem Hmida, Moncef Mokni, and Sihem Hmissa
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Upper urinary tract urothelial carcinoma ,p53 expression ,Positive surgical margin ,Survival ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The study investigated the expression and the clinicopathological significance of p53, p27, Ki-67, E-cadherin, and HER2 in upper urinary tract urothelial carcinomas (UTUC) from Tunisian patients. We performed a retrospective study of 66 UTUC. Main clinicopathological features were reported. The expression of p53, p27, Ki-67, E-cadherin, and HER2 was investigated by immunohistochemistry on whole tissue section. Results Expression of p53, Ki-67, p27, E-cadherin, and HERE2 was reported in 36.4%, 69.7%, 90.9%, 100%, and 0% of cases, respectively. p53 expression was associated with stage (p = 0.001), positive surgical margin (p = 0.005), and shorter recurrence-free survival (RFS; Log Rank test, p = 0.026). Ki-67 and p27 expression was associated with stage (p < 0.001 and p = 0.001, respectively) and grade (p < 0.001 and p = 0.001, respectively). Using Kaplan-Meier test, the positive surgical margin was associated with shorter RFS compared to free surgical margin (Log Rank test, p = 0.031). Moreover, in univariate Cox regression analysis, surgical margin (p = 0.041; HR 0.325, 95% CI 0.110–0.956) and p53 expression (p = 0.035; HR 0.328, 95% CI 0.116–0.925) were the significant factors associated with RFS. Conclusions Together, our findings suggest that positive surgical margin and p53 expression were potential prognostic factors of UTUC since both were associated with shorter RFS in Tunisian patients.
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- 2020
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12. Adenocarcinoma of intestinal type arising in mature cystic teratoma of ovary: A diagnostic dilemma
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Imtinene Belaid, Wiem Khechine, Atef Ben Abdelkader, Ahlem Bedioui, Faten Ezzairi, Imene Chabchoub, Ryma Boujnah, Taghrid Tlili, Makrem Hochlaf, Leila Ben Fatma, Moncef Mokni, and Slim Ben Ahmed
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adenocarcinoma ,dermoid cyst ,diagnosis ,Medicine ,Medicine (General) ,R5-920 - Abstract
Abstract Adenocarcinomas of intestinal type arising in mature cystic teratoma of ovary (MCT) are extremely rare and remain a diagnostic dilemma because of its similarities with MCT. Serum tumor markers CEA and SCC and also MRI may help in the preoperative diagnosis. Pathologist experience helps in intraoperative diagnosis.
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- 2020
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13. Signification of forkhead box A1 (FOXA1) expression in thyroid cancers
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Nabiha Missaoui, Sameh Chouaibi, Sarra Limam, Nozha Mhamdi, Thouraya Zahmoul, Hajer Hamchi, Moncef Mokni, and Sihem Hmissa
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Cancer ,Thyroid ,FOXA1 ,C cell ,Diagnosis ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Forkhead box A1 (FOXA1) plays an important role in several tumors. This study investigated the potential role of FOXA1 expression in thyroid tumors. We conducted a retrospective study of 110 thyroid lesions and tumors diagnosed during 1995–2018. The expression of FOXA1 was analyzed by immunohistochemistry on archival material. Results No FOXA1 immunostaining was observed in all cases of Graves’ disease, Hashimoto’s disease, multi-nodular goiter, and adenoma. FOXA1 expression was absent as well in all papillary and follicular carcinomas, Hurthle cell carcinoma, and undifferentiated sarcoma. Only three anaplastic carcinomas exhibited focally FOXA1 staining. However, FOXA1 was expressed in all medullary carcinomas. No significant correlation was found with all clinicopathological features (p > 0.05 for all). The pattern of FOXA1 staining was similar to that of calcitonin and chromogranin A (p = 0.04 and p = 0.003, respectively). Conclusions FOXA1 is expressed mostly in all medullary thyroid carcinomas. Hence, FOXA1 could serve as an additional marker for refining the diagnosis of medullary thyroid carcinoma.
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- 2019
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14. Extranodal NK/T-cell lymphoma in Tunisia: clinicopathological features, immunophenotype and EBV infection
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Nabiha Missaoui, Sarra Mestiri, Aida Bouriga, Nihed Abdessayed, Mouna Belakhdher, Monia Ghammem, Mohamed Abdelkefi, Moncef Mokni, and Sihem Hmissa
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Extranodal NK/T-cell lymphoma ,Nasal ,EBV ,Immunophenotype ,Tunisia ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Extranodal NK/T-cell lymphomas (ENKTL) are rare non-Hodgkin’s lymphomas with aggressive clinical behavior. ENKTL are frequently associated with the Epstein-Barr virus (EBV). Data on ENKTL in Africa and Arab world are extremely limited. The study investigated the clinicopathological characteristics, EBV infection, and immunophenotype of ENKTL in Tunisia. We conducted a retrospective study of ENKTL. Main clinicopathological features were reported. The expression of CD3, CD4, CD5, CD8, CD20, CD56, CD57, and Granzyme B were analyzed by immunohistochemistry. EBV infection was detected by IHC (LMP-1) and Epstein-Barr encoding region (EBER1/2) in situ hybridization. Results A total of nine ENKTL were identified (mean age of 48 years and male-to-female ratio of 8:1). There were five nasal ENKTL, and the remaining four cases had extranasal involvement (palate, sub-mandibular gland, skin, and soft tissues of the ankle). The histopathology showed a lymphoid and pleomorphic proliferation characterized by images of angiocentrism. Strong and diffuse CD3 expression was observed in all cases. Tumor cells exhibited an expression of CD5 (two cases), CD8 (three cases), CD56 (six cases), CD57 (three cases), and Granzyme B (eight cases). All ENKTL cases were EBV-associated. Overall 5-year survival rate was 57%. Although six ENKTL were diagnosed at early clinical stages, the prognosis was unfavorable and associated with patient death in three cases. Conclusions ENKTL are exceptional in Tunisia with unfavorable outcome. Histopathological diagnosis remains challenging in clinical practice. However, a careful histopathological examination combined with a correct interpretation of immunohistochemistry and in situ hybridization results refines the ENKTL diagnosis.
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- 2019
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15. La tumeur ad´nomatoïde odontogène: à propos de deux observations
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Ahlem Bchir, Ahlem Bdioui, Sarra Mestiri, Samia Ayachi, Hbib Khochtali, Sihem Hmissa, and Moncef Mokni
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tumeur adenomatoïde odontogène ,bénigne ,tumeurs odontogéniques ,rapport de cas ,Medicine - Abstract
La tumeur adénomatoïde odontogène est une tumeur épithéliale bénigne, qui touche essentiellement la femme jeune, elle siège habituellement dans la partie antérieure du maxillaire supérieur, Son du diagnostic peut être suspecté cliniquement devant une formation kystique, associée à une dent incluse, mais la confirmation repos sur l´examen histopathologie. Il s´agit de deux patients précédemment de 13 et de 37 ans, sans antécédents, qui consultaient suite à une tuméfaction siégeant respectivement au niveau du maxillaire et au niveau de la mandibule. L´examen anatomo-pathologique de ces lésions avait conclu à une tumeur adénomatoïde odontogène. A travers ces deux observations, nous rapportons les caractéristiques anatomo-cliniques, l´évolution et le traitement de ce type de tumeur.
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- 2021
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16. Primary adenoid cystic carcinoma of the lung: A case report and literature review
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Marwa Krifa, Ahlem Bdioui, Zainab Lajmi, Nabiha Missaoui, Sihem Hmissa, and Moncef Mokni
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Primary adenoid cystic carcinoma ,Lung ,Diagnosis ,Prognosis ,Immunochemistry ,Science (General) ,Q1-390 ,Social sciences (General) ,H1-99 - Abstract
Primary adenoid cystic carcinoma (ACC) of the lung is an unusual thoracic neoplasm with slow growing and low-grade malignancy. Usually, it is diagnosed at a higher clinical stage and is difficult to resect due to its central location. Herein, we report a 56-year-old man with hemoptysis associated with dyspnea and weight loss lasting for one month. Bronchial fibroscopy highlighted a budding nodular tumor in the left main bronchus. The patient underwent a left pneumonectomy with mediastinal lymphadenomectomy. Microscopic examination showed tumor cells infiltrating the bronchial wall and the cartilage and concluded to an ACC of the left bronchus. Ear, nose, and throat examination as well as cervico-facial magnetic resonance imaging were performed to search a primary salivary gland tumor and were returned without abnormalities. The tumor was classified as a primary ACC of the left bronchus without lymph node metastasis. To avoid their misdiagnosis, ACCs of the lung should be well known by the pathologist and surgeons. Their pathological features may be misleading and referring to a benign lesion, however, the presence of cribriform foci and infiltrative pattern are very suggestive. Although, indolent and slow growing tumor, long-term recurrences are quite frequent, especially in case of unclear surgical margin.
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- 2021
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17. Soft tissue huge solitary neurofibroma in the sacral region without neurofibromatosis: a pediatric case report
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Meriem Braiki, Mohamed Azzaza, Moncef Mokni, Khaled Sakly, Dorra Daly, and Fethi Derbel
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neurofibromas ,soft tissue ,surgery ,histopathology ,Medicine - Abstract
Neurofibromas are thick and irregular benign neural sheath tumors touching the peripheral nerve and it may occur at any point along a nerve. Neurofibromas occur frequently as a neurofibromatosis manifestation and less commonly solitary, in unusual sites without neurofibromatosis. The imaging especially MRI is relatively helpful to determine radiological features necessary for the diagnosis, but the definitive diagnosis is established basing on the histopathological examination of the specimen. The primary therapeutic approach for neurofibromas is a complete surgical removal to prevent tumor recurrence. This article highlights a rare case of unusual soft tissue huge solitary neurofibroma in the sacral region without neurofibromatosis in a 12-old-girl.
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- 2020
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18. A pelvic pleomorphic hyalinizing angiectatic tumor: a rare case report
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Meriem Braiki, Mohamed Azzaza, Moncef Mokni, Khaled Sakly, Dorra Daly, and Fethi Derbe
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pleomorphic ,tumor ,pelvis ,hyalinizing ,surgery ,immunohistochesmistry ,Medicine - Abstract
Pleomorphic hyalinizing angiectatic tumor (PHAT) is a particular rare entity corresponding to soft tissue neoplasm with low malignant potential. The exact etiopathology responsible for the tumor occurrence is not exactly identified. Knowledge of this condition is necessary to avoid misdiagnosis of the lesion and subsequently, to adopt the proper management. Here we report a case of PHAT originating from soft tissue in the pelvic retroperitoneum. According to the litterature, few articles deal with such pathology and such location. This work illustrates features of the tumor, its characteristics, and the adequate management with the histopathological findings.
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- 2019
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19. Membranous nephropathy succeeding autologous hematopoietic stem cell transplant: a case report
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Sanda Mrabet, Narjess Ben Aicha, Nihed Abdessayed, Moncef Mokni, and Abdellatif Achour
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Membranous nephropathy ,Glomerulonephritis ,Autologous hematopoietic stem cell transplantation ,Graft-vs-host disease ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract Background Membranous nephropathy (MN), the leading cause of nephrotic syndrome in adults, is characterized by the deposition of subepithelial immune deposits. Most of the cases are primary, while only approximately 25% of the cases are secondary to some known diseases. Recently, MN has been considered to be a possible presentation of chronic graft-versus-host disease (GVHD) of the kidney in allogeneic hematopoietic stem cell transplantation (HSCT) patients. In autologous HSCT populations, there have been scarce reports of associated MN, as a result of immune dysregulation leading to systemic autoimmunity and miming chronic GVHD. Case presentation We report an exceptional case of MN associated to an acute renal failure occurring within days following an autologous HSCT indicated by multiple myeloma. There was no evidence of GVHD or myeloma relapse. A complete remission of nephrotic syndrome with normalization of renal function were rapidly obtained by corticosteroid therapy. Conclusion This is the first published case of acute renal failure due to MN occurring in the acute phase of an autologous HSCT. These findings support the antibodymediated autoimmune glomerular disease.
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- 2018
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20. Rare triad of periampullary carcinoid, duodenal gastrointestinal stromal tumor and plexiform neurofibroma at hepatic hilum in neurofibromatosis type 1: a case report
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Nihed Abdessayed, Rahul Gupta, Sarra Mestiri, Ahlem Bdioui, Mounir Trimech, and Moncef Mokni
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Case report ,Neurofibromatosis ,Neuroendocrine tumor ,Gastrointestinal stromal tumor ,Neurofibroma ,Whipple’s operation ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background Neurofibromatosis type 1 is a relatively common inherited disorder. Patients with neurofibromatosis type 1 are at high risk of developing neurogenic, neuroendocrine and mesenchymal intra-abdominal tumors. Although coexistence of multiple tumors of different types is frequent in neurofibromatosis type 1, simultaneous occurrence of abdominal tumors of three types in very rare. Case presentation A 66-year-old lady with neurofibromatosis type 1 presented with painless progressive jaundice for six months. Laboratory investigations revealed iron deficiency anemia and conjugated hyperbilirubinemia. Tumor markers were normal. Abdominal computed tomography showed a 3 × 2 cm heterogenous mass in the periampullary region with mild dilation of the common bile duct and another 2 × 1.7 cm mass in the fourth portion of the duodenum. Endoscopic biopsy confirmed the diagnosis of periampullary carcinoid. At surgery, multiple small nodules were detected at the hepatic hilum. Frozen section suggested them to be neurofibromas. Patient underwent pancreatoduodenectomy and had uneventful recovery with no recurrence at two months. Microscopic examination of the resected specimen confirmed presence of three tumors: periampullary well differentiated neuroendocrine tumor, gastrointestinal stromal tumor of the fourth part of duodenum and plexiform neurofibroma at the hepatic hilum. Conclusion Patients of neurofibromatosis type 1 with abdominal symptoms should be treated with high index of clinical suspicion and thoroughly evaluated to rule out multiple tumors.
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- 2017
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21. Soft tissue leiomyosarcoma—diagnostics, management, and prognosis: Data of the registry cancer of the center of Tunisia
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Sarra Mestiri, Mohamed Amine Elghali, Rym Bourigua, Nihed Abdessayed, Salsabil Nasri, Ben Abdallah Amine, Nabiha Missaoui, Mahmoud Ben Maitig, Sihem Hmissa, Badreddine Sriha, and Moncef Mokni
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Background: Soft tissue leiomyosarcomas are rare, accounting for almost 5%–10% of all soft tissue sarcomas; they account for almost 1% of all sarcomas. They are aggressive tumors where location, size, and management require a multidisciplinary approach. Since there are few series published, we here analyze epidemiological pattern, clinical and pathologic features of soft tissue leiomyosarcomas. Methods: We conducted a retrospective study of 29 consecutive cases of histologically proven soft tissue leiomyosarcoma extracted from the database of the Cancer Registry of the Center of Tunisia and the Department of Pathology of Farhat Hached University Hospital of Sousse of Tunisia, during a 10-year period (from January 1996 to December 2005). Epidemiologic details, clinico-pathological features, and treatment modalities were assessed with focus on patients’ 5-year overall survival, tumor relapse, and metastases. Results: Soft tissue leiomyosarcoma accounted for 17.5% of all soft tissue sarcomas diagnosed at our pathology department. Most of patients were of advanced age (median: 52 years), with extremes ranging from 12 and 87 years. There was a slight male predominance (sex-ratio = 1.07). Tumors were located mostly in the lower limbs (45%). Deep sites as retroperitoneum was found only in two cases. Tumor size was more than 5 cm in 83% of cases (average size = 9.4 cm). Five cases had metastasis on initial staging. For 24 patients, the disease was locally limited at the moment of diagnosis. Palliative chemotherapy was indicated for four patients and surgery was performed for 20 patients. Local recurrence occurred in 11 patients (55% of operated patients) and metastasis in 6 patients. Overall, 5-year survival was about 24%. Conclusion: Our study results highlight the scarcity of soft tissue leiomyosarcoma. Unfortunately, unusual tumor sites, disease’s advanced stages, and intralesional resection made the prognosis poorer than in other series. Clinical course of soft tissue leiomyosarcoma was highly marked by local recurrence and metastasis.
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- 2019
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22. Atypical Cutaneous Fibrous Histiocytoma: An Unusual and Misleading Variant of Fibrous Histiocytoma
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Soumaya Ben Abdelkrim, Colondane Belajouza, Wafa Jomaa, Nadia Beizig, Zeineb Ben Said, Moncef Mokni, Rafia Nouira, and Badreddine Sriha
- Subjects
Pathology ,RB1-214 - Abstract
Atypical fibrous histiocytoma is a distinctive variant of cutaneous fibrous histiocytoma, which is often mistaken histologically for sarcoma and which have a tendency to recur locally and a capacity to metastasize, although very rarely. We report a new case of atypical cutaneous fibrous histiocytoma in a 31-year-old man who presented with a recurrent polypoid nodule on the abdominal wall. The diagnosis was made on the basis of morphological and immunohistochemical findings. We discuss through this case and a review of the literature pathological and evolutive features and diagnostic difficulties of this entity.
- Published
- 2011
- Full Text
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23. Combined toxicity of Cd and 2,4-dichlorophenoxyacetic acid on the earthworm Eisenia andrei under biochar amendment
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Iteb, Boughattas, Nesrine, Zitouni, Marouane, Mkhinini, Omayma, Missawi, Sondes, Helaoui, Sabrine, Hattab, Moncef, Mokni, Noureddine, Bousserrhine, and Mohamed, Banni
- Subjects
Health, Toxicology and Mutagenesis ,Environmental Chemistry ,General Medicine ,Pollution - Abstract
Due to anthropogenic activities, various pollutants can be found in agricultural soil, such as cadmium (Cd) and 2,4-dichlorophenoxyacetic acid (2,4-D). They are highly toxic and can have a negative impact on soil fertility. For remediation strategies, biochar has acquired considerable attention due to its benefits for agriculture. However, we should recognize the ecological risk posed by biochar use. In addition, little is known about its non-desirable effects on soil organisms such as earthworms, especially in the case of soil remediation. In this study, earthworms (Eisenia andrei) were exposed to soil contaminated with Cd (0.7 mg/kg), (2,4-D) (7 mg/kg), and a mixture of the two in the presence and absence of biochar (2%). A 7- and 14-day incubation experiment was carried out for this purpose. Cd and 2,4-D uptakes in earthworms' tissues, oxidative stress, cytotoxic response, DNA damage, histopathological changes, and gene expression level were assessed. Results suggested that biochar increased the bioavailability of Cd and 2,4-D and the frequency of micronuclei (MNi) and decreased the lysosomal membrane stability (LMS) in earthworms. Also, histopathological examination detected numerous alterations in animals exposed to the contaminants without any amelioration when biochar was added. The biochemical response of earthworms in terms of oxidative stress demonstrates that in the presence of biochar, animals tend to alleviate the toxicity of Cd and 2,4-D. This was also supported by transcriptomic analyses where expression gene levels related to oxidative stress were upregulated in earthworms exposed to Cd and 2,4-D + biochar. The present investigation brought new insights concerning the use of biochar in agriculture.
- Published
- 2022
24. Clinicopathological and prognostic significance of DNA mismatch-repair protein expression in gastric adenocarcinoma
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Nabiha, Missaoui, Nozha, Mhamdi, Hanene, Saad, Ahlem, Bdioui, Nihed, Abdessayed, Atef, Ben Abdelkader, Thouraya, Zahmoul, Hajer, Hamchi, Sihem, Hmissa, and Moncef, Mokni
- Subjects
Stomach Neoplasms ,Humans ,DNA ,General Medicine ,Adenocarcinoma ,Prognosis ,DNA Mismatch Repair ,Retrospective Studies - Abstract
Although the significance of DNA mismatch repair (MMR) protein expression in colorectal cancer is well-established, it remains contentious in extra-colorectal cancers and mainly in gastric adenocarcinoma. Data from Africa and Arab world remain limited. This study explored the MMR expression in gastric adenocarcinoma and evaluated its clinicopathological and prognostic signification among Tunisian patients. A retrospective study of 72 gastric adenocarcinomas was carried out. Clinicopathological particularities and patient outcomes were recorded. MMR expression was determined by immunohistochemistry on whole sections of archived material. Survival analysis was realized utilizing the Kaplan-Meier estimates and Log-Rank test. Expression of MMR proteins was observed in 84.7% of gastric adenocarcinoma samples. The 11 remaining samples (15.3%) exhibited an altered pattern of MMR protein. A significant association was identified between deficient MMR expression and advanced age (p = 0.03), intestinal type (p = 0.04) and lymph node metastases (p = 0.04). No other significant relationship was observed with the remaining selected tumor features. Patient survival was significantly associated with lymph node invasion (p = 0.002), distant metastases (p = 0.02) and tumor differentiation (p = 0.03), but not with MMR status (p = 0.83). MMR deficiency was related to advanced-age, intestinal type and nodal metastasis, but not to survival of Tunisian patients with gastric adenocarcinoma. Larger multicenter studies with additional molecular investigation are required to more explore these tumors.Bien que l’importance de l’expression des protéines de réparation des mésappariements de l’ADN (MMR) dans le cancer colorectal soit bien établie, elle reste controversée dans les cancers extra-colorectaux et principalement dans l’adénocarcinome gastrique. Les données de l’Afrique et du monde arabe restent limitées. Cette étude a exploré l’expression des protéines MMR dans l’adénocarcinome gastrique et a évalué sa signification clinicopathologique et pronostique chez les patients tunisiens. Une étude rétrospective de 72 adénocarcinomes gastriques a été réalisée. Les particularités clinicopathologiques et pronostiques des patients ont été enregistrées. L’expression des protéines MMR a été déterminée par immunohistochimie. L’analyse de survie a été réalisée en utilisant la méthode de Kaplan-Meier et le test Log-Rank. L’expression des protéines MMR a été observée dans 84,7 % des échantillons d’adénocarcinome gastrique. Les 11 cas restants (15,3 %) présentaient un profil d’expression altérée des protéines MMR. Une association significative a été identifiée entre l’expression déficiente de MMR et l’âge avancé (p = 0,03), le type intestinal (p = 0,04) et les métastases ganglionnaires (p = 0,04). Aucune autre relation significative n’a été observée avec les autres caractéristiques tumorales sélectionnées. La survie des patients était significativement associée à l’envahissement des ganglions lymphatiques (Log Rank, p = 0,002), aux métastases à distance (Log Rank, p = 0,02) et à la différenciation tumorale (Log Rank, p = 0,03), mais pas à l’expression de MMR (Log Rank, p = 0,03). Rang, p = 0,83). Le déficit de l’expression des protéines MMR était lié à l’âge avancé, au type intestinal et aux métastases ganglionnaires, mais pas à la survie des patients tunisiens ayant un adénocarcinome gastrique. Des études multicentriques avec des investigations moléculaires supplémentaires sont nécessaires pour explorer davantage le cancer gastrique avec expression déficiente des protéines MMR.
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- 2022
25. Brain injury, genotoxic damage and oxidative stress induced by Bromuconazole in male Wistar rats and in SH-SY5Y cell line
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Karima Rjiba-Touati, Hiba Hamdi, Asma M’nassri, Siwar Rich, Moncef Mokni, and Salwa Abid
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Health, Toxicology and Mutagenesis ,Clinical Biochemistry ,Biochemistry - Published
- 2022
26. Thrombotic microangiopathy due to acquired complement factor I deficiency in a male receiving interferon-beta treatment for multiple sclerosis
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Sanda Mrabet, Rihem Dahmane, Boukadida Raja, Asma Fradi, Narjess Ben Aicha, Wissal Sahtout, Awatef Azzabi, Yosra Guedri, Dorsaf Zellama, Abdellatif Achour, Imen Sfar, Rim Goucha, Nihed Abdessayed, and Moncef Mokni
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Pharmacology ,Pharmacology (medical) - Abstract
Interferon-beta, the most widely prescribed medication for multiple sclerosis, is generally considered safe. Nevertheless, rarely serious and/or life-threatening side effects have been reported such as thrombotic microangiopathy. A few mechanisms have been proposed to explain how interferon causes thrombotic microangiopathy, but insufficient immunological studies have been unable to pin this phenomenon down to a single pathophysiologic pathway. We report thrombotic microangiopathy due to acquired complement factor I deficiency in a male receiving interferon-beta treatment for multiple sclerosis. After three years of starting the therapy, the 28-year-old patient presented with malignant hypertension causing seizures, rapidly progressive renal failure requiring hemodialysis, and hemolytic anemia. Corticosteroid and plasma exchange sessions permitted hemolysis control. Nonetheless, the patient remained hemodialysis-dependent. Exploration of the complement system found a complement factor I deficiency whose activity normalized at the control carried out after two years. We concluded that IFNβ treatment may cause complement factor I deficit, which can lead to thrombotic microangiopathy and severe renal failure.
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- 2022
27. Pulmonary alveolar capillary dysplasia in infants: A rare and deadly missed diagnosis
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M. K. Souguir, Said Saadi, M. Jedidi, M. Zemni, M. Ben Dhiab, A. Ben Daly, Moncef Mokni, and T. Masmoudi
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Alveolar capillary dysplasia ,medicine.medical_specialty ,Pathology ,Missed Diagnosis ,business.industry ,Infant, Newborn ,Infant ,Autopsy ,Missed diagnosis ,030204 cardiovascular system & hematology ,medicine.disease ,Persistent Fetal Circulation Syndrome ,Pulmonary vein ,Pulmonary Alveoli ,03 medical and health sciences ,0302 clinical medicine ,Pulmonary Veins ,Dysplasia ,Epidemiology ,medicine ,Humans ,Pulmonary parenchyma ,Cardiology and Cardiovascular Medicine ,business ,Histological examination - Abstract
The pulmonary alveolocapillary dysplasia (ACD) with pulmonary vein misalignment (PVM) is a rare condition characterized by a congenital anomaly of the development of the pulmonary parenchyma. We present a case of an 8-month-old infant who died quickly from acute respiratory failure complicating an unknown ACD. We also describe its epidemiological characteristics in infants and we discuss the diagnosis's difficulties. In this case, a pulmonary arterial hypertension was decompensated by an infection. A medico-legal autopsy was performed. As for the Histological examination, it showed the features of ACD/PVM.
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- 2022
28. COVID-19 encephalitis as a differential diagnosis of a Cyclosporine related Posterior Leukoencephalopathy Syndrome (PRES)
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sanda mrabet, Achraf Jaziri, maha araoud, Wissal Sahtout, Dorsaf Zellama, Abdellatif Achour, Nihed Abdessayed, Moncef Mokni, Salma Naija, Sana Ben Amor, Alaa Souissi, and Hela Jemni
- Abstract
PRES is a rare neurological disease possibly associated with the use of calcineurin inhibitors like cyclosporine A. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), a novel coronavirus, is responsible for the outbreak of coronavirus disease 19 (COVID-19) and can cause neurological manifestations. We describe a case of CSA-related PRES whose diagnosis was difficult due to concurrent infection with SARS-CoV-2. The 16-year-old patient was known to have corticosteroid-resistant nephrotic syndrome secondary to minimal change disease. CSA was therefore introduced and on the fifth day of treatment, he presented with seizures followed by fever. Biological and MRI data were in favor of SARS-CoV-2 encephalitis. Relief of immunosuppression by discontinuation of CSA was decided and the patient was put on anticonvulsants. After being declared cured of COVID-19, which was without other clinical signs, the CSA was reintroduced but the patient presented with seizures the next day. This allowed us to rectify the diagnosis and relate the seizures to a CSA-related PRES. We concluded that infection with SARS-CoV-2 could be a differential diagnosis of a PRES related to anticalcineurins.
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- 2022
29. Sarcoid-like reaction in the kidney following rituximab for mantle lymphoma
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sanda mrabet, Asma Fradi, Wissal Sahtout, Awatef Azzabi, Raja Boukadida, Narjess Ben Aicha, Yosra Guedri, Dorsaf Zellama, Abdellatif Achour, Nihed Abdessayed, and Moncef Mokni
- Abstract
The sarcoid-like reaction is a rare autoinflammatory disease that can affect lymph nodes or organs but does not meet the diagnostic criteria for systemic sarcoidosis. Several drug classes have been associated with the development of a systemic sarcoid-like reaction, which defines drug-induced sarcoidosis-like reactions and can affect a single organ. Anti-CD20 antibodies (rituximab) have rarely been reported as responsible for this reaction and this adverse effect has mainly been described during the treatment of Hodgkin’s lymphoma. We report a unique case of a sarcoid-like reaction complicating rituximab following the treatment of a mantle cell lymphoma and interesting only the kidney. The 60-year-old patient presented with severe acute renal failure six months after the end of his r-CHOP protocol and the urgent renal biopsy revealed acute interstitial nephritis rich in granulomas without caseous necrosis. After ruling out other causes of granulomatous nephritis, a sarcoid-like reaction was retained since infiltration was limited to the kidney. The temporal relationship between rituximab administration and the sarcoid-like reaction onset in our patient supported the diagnosis of a rituximab-induced sarcoidosis-like reaction. Oral corticosteroid treatment led to rapid and lasting improvement in renal function. Clinicians should be warned of this adverse effect and regular and prolonged monitoring of renal function should be recommended during the follow-up of patients after the end of treatment with rituximab.
- Published
- 2022
30. Evaluation of hepatotoxicity and nephrotoxicity induced by fenpyroximate in subchronic-orally exposed Wistar rats
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Imen Ayed-Boussema, Karima Rjiba, Hiba Hamdi, Asma M’nassri, Awatef Azzebi, Moncef Mokni, and Salwa Abid
- Subjects
Superoxide Dismutase ,Health, Toxicology and Mutagenesis ,Clinical Biochemistry ,Kidney ,Biochemistry ,Antioxidants ,Rats ,Uric Acid ,Oxidative Stress ,Liver ,Creatinine ,Malondialdehyde ,Animals ,Rats, Wistar ,Chemical and Drug Induced Liver Injury ,Acaricides ,Biomarkers - Abstract
Fenpyroximate (FEN) is an acaricide that inhibits the complex I of the mitochondrial respiratory chain. The aim of this work was to explore the hepatotoxic and nephrotoxic effects of FEN on Wistar rats.The study involved five groups: a control group and four groups treated with FEN at 1, 2, 4, and 8 mg/Kg bw for 28 consecutive days. Histological examination and biochemical analysis of hepatic and renal biomarkers were performed. The malondialdehyde (MDA), protein carbonyl levels, and antioxidant enzymes activities were measured. Comet assay was conducted to explore FEN genotoxicity.FEN induced a disturbance of the hepatic and renal functions as evidenced by an increase in AST, ALT, ALP, creatinine, and uric acid levels and histopathological modifications in the two examined tissues. FEN increased hepatic and renal lipid peroxidation and protein oxidation. The activities of liver and kidney SOD, CAT,FEN was hepatotoxic and nephrotoxic very likely through induction of oxidative stress.
- Published
- 2022
31. Inhabitual presentation of Sertoli-Leydig cell tumor of the ovary with xeroderma pigmentosum: Case report with review of literature
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Sihem Hmissa, Moncef Mokni, Nabiha Missaoui, Hajer Hamchi, Ahlem Bdioui, and Ahlem Bchir
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,endocrine system ,Pathology ,medicine.medical_specialty ,Abdominal pain ,Xeroderma pigmentosum ,Ovary ,Adnexal mass ,03 medical and health sciences ,0302 clinical medicine ,Rhabdomyomatous differentiation ,Case report ,Ascites ,Medicine ,Basal cell carcinoma ,skin and connective tissue diseases ,Sertoli-Leydig Cell Tumor ,Sertoli-Leydig cell tumor ,urogenital system ,business.industry ,nutritional and metabolic diseases ,Heterologous elements ,medicine.disease ,Regimen ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,030211 gastroenterology & hepatology ,Surgery ,medicine.symptom ,business - Abstract
Highlights • Sertoli-Leydig cell tumors are rare sex-cord stromal tumors of the ovary. • We present a case of Sertoli-Leydig cell tumors with rhabdomyomatous differentiation on the setting of xeroderma pigmentosum. • We describe clinical, gross and microscopic examinations for diagnosis and treatment., Introduction Sertoli-Leydig cell tumors (SLCTs) are rare sex-cord stromal tumors of the ovary. Heterologous components may be present, most commonly in the intermediate differentiated and poorly differentiated groups. Because of their scarcity, SLCTs with heterologous differentiation represent a challenge in both diagnosis and management, with limited available experience. Presentation of case We report a case of a 27-year-old, Tunisian woman, followed in the Dermatology Department since the age of six months for xeroderma pigmentosum, with a history of basal cell carcinoma of the face operated on several times. The patient presented with abdominal pain and bloating associated with a medium abundance ascites on physical exam. Ultrasound showed a large left adnexal mass associated with an elevated cancer antigen 125 on serological exam. The patient underwent unilateral salpingo-oophorectomy with resection of two omental nodules. Microscopic examination concluded to poorly differentiated Sertoli-Leydig tumor with rhabdomyomatous differentiation. Adjuvant chemotherapy was performed and there was no clinical evidence of tumor recurrence during the three years of follow-up. Discussion SLCTs with rhabdomyomatous differentiation on the setting of xeroderma pigmentosum are exceptional, microscopic diagnosis and management is challenging, considering the tumor scarcity. Conclusion Further case reports and retrospective studies are required to more understand the pathogenesis of SLCTs and to determine their optimal treatment regimen.
- Published
- 2021
32. Fenpyroximate induced cytotoxicity and genotoxicity in Wistar rat brain and in human neuroblastoma (SH-SY5Y) cells: Involvement of oxidative stress and apoptosis
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Imen Ayed-Boussema, Hiba Hamdi, Hanen Chaabani, Asma M’nassri, Moncef Mokni, and Salwa Abid
- Subjects
Cell Survival ,Superoxide Dismutase ,General Neuroscience ,Brain ,Apoptosis ,Toxicology ,Benzoates ,Rats ,Neuroblastoma ,Oxidative Stress ,Cell Line, Tumor ,Acetylcholinesterase ,Animals ,Humans ,Pyrazoles ,Rats, Wistar ,DNA Damage - Abstract
Fenpyroximate (FEN) is an acaricide used in agriculture / horticulture to control spider mites and leafhoppers. It inhibits the transport of mitochondrial electrons at the level of NADH-coenzyme Q oxidoreductase (complex I). Despite the implication of inhibition of mitochondrial complex I in neurotoxicity, especially in neurodegenerative diseases, data concerning FEN neurotoxicity remain limited. Thus, the present study was designed to investigate the toxic effect of FEN on rat brain tissue and on human neuroblastoma cells (SH-SY5Y). Rat exposure to FEN at three different doses (4.8, 9.6 and 48 mg / Kg bw) for 28 consecutive days resulted in histopathological modifications in brain tissue and a significant decrease in acetylcholinesterase activity. Further, FEN significantly enhanced lipid peroxidation and protein oxidation in rat brain and disturbed activities of antioxidant enzymes (SOD, CAT, GPx, and GST). Besides, FEN was found to induce DNA damage in a significant and dose-dependent manner in rat brain as assessed by the comet assay. To better understand FEN neurotoxic effect, we monitored our study on SH-SY5Y cells. We confirm our data found in rat brain tissue. In fact, FEN induced cell mortality in a concentration dependent manner. It over-produced intracellular ROS and lipid peroxidation and enhanced SOD and CAT activities. FEN was also found to induce DNA damage in SH-SY5Y cells. Moreover, FEN induced a loss of mitochondrial membrane potential, which confirms FEN mitochondrial impairing activity. Acridine Orange-Bromure Etidium (AO-BE) cell staining indicated that FEN enhanced the percentage of apoptotic cells in a concentration dependent manner. Further, pretreatment with a general caspases inhibitor (ZVAD-FMK), reduced significantly the FEN induced cell mortality. We also shown that FEN increased caspase 3 activity. These findings suggested, for the first time, the possibility of the involvement of mitochondrial pathway in FEN-induced cell apoptosis.
- Published
- 2022
33. Les tumeurs malignes de type glandes salivaire primitives du poumon: étude clinico-pathologiques de 10 cas
- Author
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Ahlem Bchir, Slim Ben Ahmed, and Moncef Mokni
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General Medicine - Published
- 2022
34. Sudden Death and Pheochromocytoma: How is SARS-CoV-2 Incriminated?
- Author
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Amal ben Daly, Nihed Abdessayed, maher Jdidi, mohamed amine Zaara, Zeineb Nfikha, Mohamed Ben Dhiab, and Moncef Mokni
- Subjects
History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
35. Significance of p53, p27, Ki-67, E-cadherin, and HER2 expression in upper urinary tract urothelial carcinoma
- Author
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Moncef Mokni, Wissem Hmida, Sihem Hmissa, Atika Baccouche, Ahlem Bdioui, Oussema Belkacem, and Nabiha Missaoui
- Subjects
Cancer Research ,Surgical margin ,medicine.medical_specialty ,p53 expression ,Survival ,Receptor, ErbB-2 ,030232 urology & nephrology ,Positive surgical margin ,Gastroenterology ,lcsh:RC254-282 ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,Kidney Pelvis ,Stage (cooking) ,Upper urinary tract ,Retrospective Studies ,Carcinoma, Transitional Cell ,biology ,Proportional hazards model ,business.industry ,Upper urinary tract urothelial carcinoma ,Cadherins ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Log-rank test ,Ki-67 Antigen ,Oncology ,Urinary Bladder Neoplasms ,030220 oncology & carcinogenesis ,Ki-67 ,biology.protein ,Immunohistochemistry ,Positive Surgical Margin ,Tumor Suppressor Protein p53 ,business - Abstract
Background The study investigated the expression and the clinicopathological significance of p53, p27, Ki-67, E-cadherin, and HER2 in upper urinary tract urothelial carcinomas (UTUC) from Tunisian patients. We performed a retrospective study of 66 UTUC. Main clinicopathological features were reported. The expression of p53, p27, Ki-67, E-cadherin, and HER2 was investigated by immunohistochemistry on whole tissue section. Results Expression of p53, Ki-67, p27, E-cadherin, and HERE2 was reported in 36.4%, 69.7%, 90.9%, 100%, and 0% of cases, respectively. p53 expression was associated with stage (p = 0.001), positive surgical margin (p = 0.005), and shorter recurrence-free survival (RFS; Log Rank test, p = 0.026). Ki-67 and p27 expression was associated with stage (p < 0.001 and p = 0.001, respectively) and grade (p < 0.001 and p = 0.001, respectively). Using Kaplan-Meier test, the positive surgical margin was associated with shorter RFS compared to free surgical margin (Log Rank test, p = 0.031). Moreover, in univariate Cox regression analysis, surgical margin (p = 0.041; HR 0.325, 95% CI 0.110–0.956) and p53 expression (p = 0.035; HR 0.328, 95% CI 0.116–0.925) were the significant factors associated with RFS. Conclusions Together, our findings suggest that positive surgical margin and p53 expression were potential prognostic factors of UTUC since both were associated with shorter RFS in Tunisian patients.
- Published
- 2020
36. Adenocarcinoma of intestinal type arising in mature cystic teratoma of ovary: A diagnostic dilemma
- Author
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I. Belaid, Imene Chabchoub, W. Khechine, Ahlem Bedioui, Moncef Mokni, Leila Ben Fatma, Taghrid Tlili, Makrem Hochlaf, Faten Ezzairi, Ryma Boujnah, Atef Ben Abdelkader, and Slim Ben Ahmed
- Subjects
Pathology ,medicine.medical_specialty ,dermoid cyst ,diagnosis ,lcsh:Medicine ,Ovary ,Case Report ,Diagnostic dilemma ,Case Reports ,030204 cardiovascular system & hematology ,Mature Cystic Teratoma ,03 medical and health sciences ,0302 clinical medicine ,medicine ,neoplasms ,Intestinal type ,lcsh:R5-920 ,adenocarcinoma ,business.industry ,lcsh:R ,General Medicine ,medicine.disease ,digestive system diseases ,medicine.anatomical_structure ,Dermoid cyst ,030220 oncology & carcinogenesis ,Adenocarcinoma ,business ,lcsh:Medicine (General) - Abstract
Adenocarcinomas of intestinal type arising in mature cystic teratoma of ovary (MCT) are extremely rare and remain a diagnostic dilemma because of its similarities with MCT. Serum tumor markers CEA and SCC and also MRI may help in the preoperative diagnosis. Pathologist experience helps in intraoperative diagnosis.
- Published
- 2020
37. Pyoderma gangrenosum et lupus érythémateux systémique : une association rare
- Author
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I. Chelly, Moncef Mokni, A. Souissi, O. Magdoud, and Slim Haouet
- Subjects
030203 arthritis & rheumatology ,Gynecology ,medicine.medical_specialty ,Systemic lupus erythematosus ,business.industry ,Gastroenterology ,medicine.disease ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Internal Medicine ,medicine ,business ,Pyoderma gangrenosum - Abstract
Resume Introduction Le pyoderma gangrenosum (PG) est une dermatose neutrophilique classiquement associee a des maladies systemiques telles que les maladies inflammatoires chroniques de l’intestin, la polyarthrite rhumatoide et les hemopathies malignes. Son association au lupus erythemateux systemique (LES) est rare et peu connue. Nous rapportons un cas de cette association avec une revue de la litterature. Observation Une patiente âgee de 43 ans, suivie depuis 4 ans pour LES, presentait une ulceration profonde de la face anterieure de la cuisse gauche a bordure inflammatoire, un nodule ulcere de l’epaule droite et quatre petites ulcerations du dos de la main droite. La biopsie de l’ulceration de la cuisse gauche concluait a un PG. La patiente a ete traitee par corticoides avec regression complete des lesions. Conclusion Le pronostic du lupus ne semble pas etre aggrave par le PG et les traitements d’une poussee de LES suffisent habituellement pour traiter un PG associee.
- Published
- 2020
38. Gastroprotective effect of leaf extract of two varieties grapevine (Vitis viniferaL.) native wild and cultivar grown in North of Tunisia against the oxidative stress induced by ethanol in rats
- Author
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Nabil Saadaoui, Mounira Harbi, Ikram Khemiri, Moncef Mokni, Taha Barkaoui, Asma Weslati, Wafa Gadacha, Abdelaziz Souli, and Mossadok Ben-Attia
- Subjects
Folk medicine ,Ethanol ,Health, Toxicology and Mutagenesis ,fungi ,Clinical Biochemistry ,food and beverages ,030204 cardiovascular system & hematology ,Biology ,medicine.disease_cause ,Biochemistry ,03 medical and health sciences ,chemistry.chemical_compound ,Horticulture ,0302 clinical medicine ,chemistry ,030220 oncology & carcinogenesis ,medicine ,Cultivar ,Vitis vinifera ,Oxidative stress - Abstract
Context: Vitis vinifera leaves are traditionally used in Tunisian folk medicine to treat digestive pathologies.Objective: We aimed to compare the gastroprotective effects of hydromethanolic...
- Published
- 2019
39. Extranodal NK/T-cell lymphoma in Tunisia: clinicopathological features, immunophenotype and EBV infection
- Author
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Monia Ghammem, Sihem Hmissa, Mohamed Abdelkefi, Moncef Mokni, Nabiha Missaoui, Mouna Belakhdher, Nihed Abdessayed, Aida Bouriga, and Sarra Mestiri
- Subjects
Adult ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,Epstein-Barr Virus Infections ,Herpesvirus 4, Human ,Tunisia ,lcsh:RC254-282 ,Viral Matrix Proteins ,03 medical and health sciences ,0302 clinical medicine ,Immunophenotyping ,EBV ,hemic and lymphatic diseases ,Medicine ,T-cell lymphoma ,Humans ,Survival rate ,Aged ,Retrospective Studies ,CD20 ,Extranodal NK/T-cell lymphoma ,biology ,business.industry ,Nasal ,Middle Aged ,medicine.disease ,Prognosis ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Antigens, Differentiation ,Lymphoma, Extranodal NK-T-Cell ,Survival Rate ,Oncology ,030220 oncology & carcinogenesis ,biology.protein ,Immunohistochemistry ,RNA, Viral ,Histopathology ,Female ,CD5 ,business ,Immunophenotype ,CD8 ,030215 immunology - Abstract
Background Extranodal NK/T-cell lymphomas (ENKTL) are rare non-Hodgkin’s lymphomas with aggressive clinical behavior. ENKTL are frequently associated with the Epstein-Barr virus (EBV). Data on ENKTL in Africa and Arab world are extremely limited. The study investigated the clinicopathological characteristics, EBV infection, and immunophenotype of ENKTL in Tunisia. We conducted a retrospective study of ENKTL. Main clinicopathological features were reported. The expression of CD3, CD4, CD5, CD8, CD20, CD56, CD57, and Granzyme B were analyzed by immunohistochemistry. EBV infection was detected by IHC (LMP-1) and Epstein-Barr encoding region (EBER1/2) in situ hybridization. Results A total of nine ENKTL were identified (mean age of 48 years and male-to-female ratio of 8:1). There were five nasal ENKTL, and the remaining four cases had extranasal involvement (palate, sub-mandibular gland, skin, and soft tissues of the ankle). The histopathology showed a lymphoid and pleomorphic proliferation characterized by images of angiocentrism. Strong and diffuse CD3 expression was observed in all cases. Tumor cells exhibited an expression of CD5 (two cases), CD8 (three cases), CD56 (six cases), CD57 (three cases), and Granzyme B (eight cases). All ENKTL cases were EBV-associated. Overall 5-year survival rate was 57%. Although six ENKTL were diagnosed at early clinical stages, the prognosis was unfavorable and associated with patient death in three cases. Conclusions ENKTL are exceptional in Tunisia with unfavorable outcome. Histopathological diagnosis remains challenging in clinical practice. However, a careful histopathological examination combined with a correct interpretation of immunohistochemistry and in situ hybridization results refines the ENKTL diagnosis.
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- 2019
40. An unexpected sudden death due to a choroid plexus papilloma: an autopsy case report
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Dorra Chiba, Moncef Mokni, Sarra Gharsallaoui, Mohamed Amine Zaara, Nihed Abdessayed, Sarra Mestiri, and Mohamed Ben Dhiab
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Medicine (General) ,Pathology ,medicine.medical_specialty ,Health (social science) ,Choroid plexus ,Autopsy ,Fourth ventricle ,Sudden death ,Pathology and Forensic Medicine ,R5-920 ,Case report ,Medicine ,Medical history ,Cause of death ,Papilloma ,business.industry ,K1-7720 ,medicine.disease ,Choroid plexus papilloma ,Law in general. Comparative and uniform law. Jurisprudence ,business ,Complication ,Law - Abstract
BackgroundChoroid plexus papillomas (CPPs) are histopathologically benign and rare central nervous system tumors. These tumors remain more frequent in children than adults. It is infrequent for these tumors to cause a sudden unexpected death. We aim in this case to discuss the unusual and fatal presentation of choroid plexus papilloma and the mechanism of death.Case presentationwe report the case of a 61-year-old man with no medical history, diagnosed at autopsy with a previously unknown CPP. Initial complaints were chronic headache occurring in the last month and acute chest pain for two days. The forensic autopsy including the histopathologic examination showed a tumoral mass of the choroid plexus in the fourth ventricle diagnosed as a psammomatous CPP. The cause of death in this case was attributed to a massive cerebral edema caused by the tumoral mass.ConclusionsThrough this case report, we stress the importance of an early and a vigorous investigation of every headache and an early detection of this tumor and we highlight as well the role of the post mortem examination to detect such a fatal complication.
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- 2021
41. EPV027/#553 Concordance in molecular profiles of invasive breast cancer between core needle biopsy and definitive operative specimen analysis
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R Bouchahda, Moncef Mokni, M Bibi, O Kaabia, and S Hidar
- Subjects
Core needle ,medicine.medical_specialty ,Breast cancer ,medicine.diagnostic_test ,business.industry ,Concordance ,Biopsy ,medicine ,Radiology ,medicine.disease ,business - Published
- 2021
42. Metabolomic disorders unveil hepatotoxicity of environmental microplastics in wild fish Serranus scriba (Linnaeus 1758)
- Author
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Nesrine Zitouni, Tiziana Cappello, Omayma Missawi, Iteb Boughattas, Giuseppe De Marco, Sabrina Belbekhouche, Moncef Mokni, Vanessa Alphonse, Hamadi Guerbej, Noureddine Bousserrhine, Mohamed Banni, Institut de Chimie et des Matériaux Paris-Est (ICMPE), and Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)
- Subjects
Environmental Engineering ,Microplastics ,Pollution ,Polyethylene ,Acetylcholinesterase ,[CHIM]Chemical Sciences ,Animals ,Metabolomics ,Environmental Chemistry ,Bass ,Chemical and Drug Induced Liver Injury ,Plastics ,Waste Management and Disposal ,ComputingMilieux_MISCELLANEOUS ,Water Pollutants, Chemical ,Environmental Monitoring - Abstract
Coastal areas are worldwide subject to large inputs of anthropogenic wastes that are discharged directly into inshore waters, where they will be weathered into small microplastics (MPs) of up to a size20 μm. This study provides information about the presence of small environmental MPs (≤3 μm) in the liver of adult benthopelagic fish Serranus scriba (Linnaeus 1758), caught from three coastal regions in Tunisia distinguished by different patterns of human activity. Polymer composition in fish liver was identified using Raman microspectroscopy. Results revealed differences in the abundance, size distribution and presence of plastic additives over the investigated sites. Polyethylene-vinyl acetate (PEVA: 34% particles/g of tissue), high density polyethylene (HDPE: 24.4%) and the two smaller size classes, i.e. 3-1.2 μm and 1.2-0.45 μm, were the most abundant MPs types and size distribution found, respectively, in Bizerte channel (BC) site (Bizerte city, Tunisia). Moreover, at hepatic level data showed a significant site-dependent cytotoxicity expressed by changes in malondialdehyde (MDA) content, presence of reactive oxygen species (ROS) expressed by altered level of catalase (CAT) and glutathione-S-transferase (GST) activities and in the content of metallothioneins (MTs), as well as genotoxicity by changes in the amount of micronucleus (MN), and neurotoxicity by altered activity of acetylcholinesterase (AChE). A innovative metabolomics analysis was also performed to further investigate the distinct patterns of key metabolite changes in the liver of Serranus scriba. A total of 36 metabolites were significantly affected, mainly involved in energy, amino acid and osmolyte metabolism. These findings emphasised for the first time a close relationship between the source, abundance and size ranges of environmental MPs ≤ 3 μm and their hepatotoxicity in wild organisms.
- Published
- 2022
43. Exceptional thyroid cancer: a challenging diagnosis
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Ach Koussay El, Abir Meherzi, Mouna Bellakhdher, Monia Ghammem, Yosra Hasni, Mohamed Zbdelkafi, Lobna Chouchane, Moncef Mokni, Sarra Mestiri, and Wassim Kermani
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Oncology ,medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,medicine.disease ,business ,Thyroid cancer - Published
- 2021
44. Bromuconazole caused genotoxicity, hepatic and renal failure via oxidative stress process in Wistar rats
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Karima RJIBA, Hiba Hamdi, Asma M’nassri, Yosra Guedri, Moncef Mokni, and Salwa Abid
- Abstract
Bromuconazole is a triazole pesticide used to protect vegetables and fruits against diverse fungi pathologies. However, its utilization may be accompanied by diverse tissues injuries. For this, we tried to examine bromuconazole effects in liver and kidney tissues by the evaluation of biochemical and histopathological modifications also by genotoxic and oxidative stress analysis. Adult male Wistar rats were divided into four groups, each consisting of 6 animals. The control group received daily a corn oil (vehicle) orally. Three oral Bromuconazole doses were tested (1, 5 and 10 % of LD50) daily for 28 days. Bromuconazole increased the plasma activities of transaminases (AST, ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH), creatinine and uric acid levels. histopathological check showed that Bromuconazole caused organs failure. This study make known that Bromuconazole caused conspicuous DNA damage either in hepatic and kidney tissues, with a significant increase in malondialdehyde and protein carbonyl levels followed by the increase in the enzymatic activity of catalase and superoxide dismutase in a dose dependent manner. Glutathione-S-transferase (GST) and peroxidase (GPx) activities were also recorded. Our results highlight that bromuconazole exposure induced genotoxic damage and organs failure that may be caused by the disturbances of oxidative stress statue in liver and kidney tissues.
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- 2021
45. Histopathological diagnosis of strongyloidiasis hyperinfection in Tunisian patient with hodgkin lymphoma: Case report
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Ahlem Bdioui, Ahlem Bchir, Sihem Hmissa, Moncef Mokni, and Nabiha Missaoui
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medicine.medical_specialty ,Gastrointestinal infection ,Nausea ,Histopathology ,BEACOPP, Bleomycin, etoposide, doxorubicin, cyclophosphamide, vincristine, procarbazine, and prednisone ,Asymptomatic ,Strongyloides stercoralis ,03 medical and health sciences ,0302 clinical medicine ,Diagnosis ,Case report ,medicine ,Helminths ,Medical history ,biology ,business.industry ,HTLV1, Human T-lymphotropic virus ,General Medicine ,biology.organism_classification ,medicine.disease ,Dermatology ,M.O.P.P, Nitrogen mustard, oncovin, prednisone, procarbazine ,Strongyloidiasis ,HIV, Human immunodeficiency virus ,030220 oncology & carcinogenesis ,Vomiting ,030211 gastroenterology & hepatology ,Surgery ,medicine.symptom ,business ,Hodgkin lymphoma - Abstract
Introduction Strongyloides stercoralis, an intestinal nematode, is commonly dispersed throughout the tropical and subtropical regions. Strongyloides stercoralis infection typically contributes to an asymptomatic chronic disease which can remain hidden for decades. However, in immunocompromised patients, the hyperinfection can take place, causing high mortality rates. Case presentation A 45 year-old Tunisian women, with heavy medical history, suffering of stage 3 classic Hodgkin lymphoma under treatment; presented with complaints of epigastric pain, nausea, vomiting. Gastroduodenoscopy showed duodenal and gastric erythematous and ulcerated mucosa. Histological assessment showed chronic infiltration with a large amount of eosinophils around numerous helminth forms identified as larvae of Strongyloides stercoralis. Conclusion Early detection of Strongyloides stercoralis infection in immunocompromised patients is life saving and avoids fatality caused by hyperinfection or systemic dissemination. Routine stool examination may be negative, so histopathological identification of the parasite in tissue sections provides the definite diagnosis., Highlights • Strongyloides stercoralis is very rare, prevention is necessary in the immunocompromised subject.
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- 2021
46. Bromuconazole caused genotoxicity and hepatic and renal damage via oxidative stress process in Wistar rats
- Author
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Hiba Hamdi, Yosra Guedri, Asma M’nassri, Salwa Abid, Moncef Mokni, and Karima Rjiba-Touati
- Subjects
Male ,Health, Toxicology and Mutagenesis ,Pharmacology ,medicine.disease_cause ,Kidney ,Antioxidants ,Superoxide dismutase ,chemistry.chemical_compound ,medicine ,Environmental Chemistry ,Animals ,Rats, Wistar ,Furans ,biology ,business.industry ,Superoxide Dismutase ,General Medicine ,Triazoles ,Malondialdehyde ,Catalase ,Pollution ,Glutathione ,Rats ,Oxidative Stress ,medicine.anatomical_structure ,chemistry ,Liver ,biology.protein ,Uric acid ,Alkaline phosphatase ,business ,Oxidative stress ,Genotoxicity ,DNA Damage - Abstract
Bromuconazole is a triazole pesticide used to protect vegetables and fruits against diverse fungi pathologies. However, its utilization may be accompanied by diverse tissue injuries. In this study, we evaluated the biochemical and histopathological modifications, and we analyzed genotoxic and oxidative stress, in the aim to examine bromuconazole effects in the liver and kidney. We subdivided animals into four groups, each one contains six adult male Wistar rats. Untreated rats received daily a corn oil (vehicle) orally. Three oral bromuconazole doses were tested (1, 5, and 10 % of LD50) daily for 28 days. Bromuconazole increased the plasma activities of alkaline phosphatase, lactate dehydrogenase, and transaminases. It also increased the plasma levels of creatinine and uric acid. Histopathological check showed that bromuconazole caused organ damage. This study makes known that bromuconazole caused conspicuous DNA damage either in hepatic or kidney tissues, with a significant increase in the levels of malondialdehyde and protein carbonyl followed by an enhancement in catalase and superoxide dismutase enzymatic activities, and these increases are in a dose-dependent manner. In other side, we found that Glutathione-S-transferase and peroxidase activities raised. Our outcomes highlight that bromuconazole exposure induced genotoxic damage and organ damage which may be caused by the disturbances of oxidative stress statue in the liver and kidney.
- Published
- 2021
47. La tumeur adénomatoïde odontogène: à propos de deux observations
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Hbib Khochtali, Ahlem Bchir, Ahlem Bdioui, Samia Ayachi, Sarra Mestiri, Sihem Hmissa, and Moncef Mokni
- Subjects
odontogenic tumors ,Pathology ,medicine.medical_specialty ,tumeurs odontogéniques ,Retained tooth ,Adenomatoid odontogenic tumor ,business.industry ,Mandible ,Tumeur adenomatoïde odontogène ,General Medicine ,medicine.disease ,Cyst formation ,medicine ,case report ,In patient ,Benign epithelial tumor ,rapport de cas ,benign ,business ,bénigne - Abstract
La tumeur adénomatoïde odontogène est une tumeur épithéliale bénigne, qui touche essentiellement la femme jeune, elle siège habituellement dans la partie antérieure du maxillaire supérieur. Son diagnostic peut être suspecté cliniquement devant une formation kystique, associée à une dent incluse, mais la confirmation repose sur l'examen histopathologie. Il s'agit de deux patients précédemment de 13 et de 37 ans, sans antécédents, qui consultaient suite à une tuméfaction siégeant respectivement au niveau du maxillaire et au niveau de la mandibule. L'examen anatomo-pathologique de ces lésions avait conclu à une tumeur adénomatoïde odontogène. A travers ces deux observations, nous rapportons les caractéristiques anatomo-cliniques, l'évolution et le traitement de ce type de tumeur.
- Published
- 2021
48. POS-502 HISTOLOGICAL FEATURES OF LUPUS NEPHRITIS
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A. Azzabi, F. Sabri, S. Mrabet, Moncef Mokni, Asma Fradi, O. Mahfoudh, Y. Guedri, N. Ben Aicha, W. Sahtout, Nihed Abdessayed, S. Ben Amor, A. Achour, R. Boukadida, and Dorsaf Zellama
- Subjects
Pathology ,medicine.medical_specialty ,Nephrology ,business.industry ,Lupus nephritis ,medicine ,RC870-923 ,medicine.disease ,business ,Diseases of the genitourinary system. Urology - Published
- 2021
49. Sudden death due to spontaneous right ventricular rupture: exceptional complication of arrhythmogenic right ventricular cardiomyopathy
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Maher Jedidi, Sihem Hamissa, M. Zemni, Cherif El Khal, Kamel Souguir, Moncef Mokni, and Maha Sahnoun
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medicine.medical_specialty ,business.industry ,Internal medicine ,medicine ,Cardiology ,General Medicine ,Complication ,business ,Sudden death ,Right ventricular cardiomyopathy - Published
- 2019
50. Prognostic significance of MGMT methylation and expression of MGMT, P53, EGFR, MDM2 and PTEN in glioblastoma multiforme
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Sarra Mestiri, Mohamed Tahar Yacoubi, Moncef Mokni, Sarra Limam, Nihed Abdessayed, Boulbaba Selmi, and Nabiha Missaoui
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Adult ,Male ,030213 general clinical medicine ,Tunisia ,Adolescent ,Biology ,Young Adult ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Predictive Value of Tests ,Promoter methylation ,medicine ,Humans ,PTEN ,Child ,Promoter Regions, Genetic ,DNA Modification Methylases ,neoplasms ,Aged ,Retrospective Studies ,Univariate analysis ,Brain Neoplasms ,Tumor Suppressor Proteins ,PTEN Phosphohydrolase ,Proto-Oncogene Proteins c-mdm2 ,General Medicine ,Methylation ,DNA Methylation ,Middle Aged ,Prognosis ,medicine.disease ,Immunohistochemistry ,digestive system diseases ,ErbB Receptors ,Gene Expression Regulation, Neoplastic ,DNA Repair Enzymes ,chemistry ,Child, Preschool ,biology.protein ,Cancer research ,Mdm2 ,Female ,Tumor Suppressor Protein p53 ,Glioblastoma ,DNA - Abstract
The study investigated the pattern of MGMT promoter methylation and the expression of MGMT, P53, EGFR, MDM2 and PTEN proteins in glioblastomas multiforme (GBM) and evaluated their prognostic significance. We carried out a retrospective study of 80 GBM. Expression of MGMT as well as of P53, EGFR, MDM2 and PTEN was investigated by immunohistochemistry. MGMT promoter methylation was investigated by methylation specific-PCR of bisulfite-treated DNA. Twenty-five GBM exhibited MGMT expression. Methylation of MGMT promoter was detected in 35.1% of cases. No significant concordance was reported between MGMT promoter methylation and protein expression (κ=-0.047, p=0.11). MGMT promoter methylation was significantly associated only with PTEN expression (p=0.001): no other significant association was identified with clinical parameters as well as with expression of P53, EGFR and MDM2 (p >0.05). Tumor recurrence was significantly associated with unmethylated MGMT promoter (p=0.01) but not with MGMT expression (p=0.51). Recurrence-free survival (RFS) was significantly better among patients with methylated MGMT promoter (log rank, p
- Published
- 2019
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