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4. Mutagenic and genotoxic effects induced by PM0.5 of different Italian towns in human cells and bacteria: The MAPEC_LIFE study

Author

Bonetta, Sara, Bonetta, Silvia, Schiliro', Tiziana, Elisabetta, Ceretti, Donatella, Feretti, Loredana, Covolo, Samuele, Vannini, Milena, Villarini, Massimo, Moretti, Marco, Verani, Annalaura, Carducci, Francesco, Bagordo, Antonella De Donno, Silvia, Bonizzoni, Alberto, Bonetti, Pignata, Cristina, Carraro, Elisabetta, Umberto, Gelatti, MAPEC_LIFE Study Group, Gilli, G., Romanazzi, V., Gea, M., Festa, A., Viola, G. C. V., Zani, C., Zerbini, I., Donato, F., Monarca, S., Fatigoni, C., Levorato, S., Salvatori, T., Donzelli, G., Palomba, G., Casini, B., Giorgi, De, Devoti, M., Grassi, G., Idolo, T., Panico, A., Serio, A., Furia, F., Colombi, C., Bonetta, Sara, Bonetta, Silvia, Schilirò, Tiziana, Ceretti, Elisabetta, Feretti, Donatella, Covolo, Loredana, Vannini, Samuele, Villarini, Milena, Moretti, Massimo, Verani, Marco, Carducci, Annalaura, Bagordo, Francesco, De Donno, Antonella, Bonizzoni, Silvia, Bonetti, Alberto, Pignata, Cristina, Carraro, Elisabetta, Gelatti, Umberto, Gilli, G., Romanazzi, V., Gea, M., Festa, A., Viola, G. C. V., Zani, C., Zerbini, I., Donato, F., Monarca, S., Fatigoni, C., Levorato, Maria Chiara, Salvatori, T., Donzelli, G., Palomba, G., Casini, B., De Giorgi, M., Devoti, G., Grassi, T., Idolo, A., Panico, A., Serio, F., Furia, C., and Colombi, P.

Subjects
Veterinary medicine, Genotoxicity, Mutagenicity, Nitro-PAHs, PAHs, PM0.5, Toxicology, Pollution, Health, Toxicology and Mutagenesis, 010504 meteorology & atmospheric sciences, PM, 010501 environmental sciences, medicine.disease_cause, 01 natural sciences, Ames test, nitro-PAHs, Salmonella, Bioassay, Polycyclic Aromatic Hydrocarbon, Child, media_common, General Medicine, Particulates, Citie, Italy, Air Pollutant, Health, PM0.5, mutagenicity, genotoxicity,comet assy, PAHs, nitro-PAHs, comet assy, Human, Nitro-PAH, media_common.quotation_subject, Biology, medicine, Toxicology and Mutagenesis, Particle Size, A549 Cell, 0105 earth and related environmental sciences, Pollutant, Epithelial Cell, genotoxicity, mutagenicity, PAH, 0.5, biology.organism_classification, Comet assay, Mutagenicity Test, Particulate Matter, Season, Bacteria, DNA Damage
Abstract

Particulate matter (PM) is considered an atmospheric pollutant that mostly affects human health. The finest fractions of PM (PM2.5 or less) play a major role in causing chronic diseases. The aim of this study was to investigate the genotoxic effects of PM0.5 collected in five Italian towns using different bioassays. The role of chemical composition on the genotoxicity induced was also evaluated. The present study was included in the multicentre MAPEC_LIFE project, which aimed to evaluate the associations between air pollution exposure and early biological effects in Italian children. PM10 samples were collected in 2 seasons (winter and spring) using a high-volume multistage cascade impactor. The results showed that PM0.5 represents a very high proportion of PM10 (range 10–63%). PM0.5 organic extracts were chemically analysed (PAHs, nitro-PAHs) and tested by the comet assay (A549 and BEAS-2B cells), MN test (A549 cells) and Ames test on Salmonella strains (TA100, TA98, TA98NR and YG1021). The highest concentrations of PAHs and nitro-PAHs in PM0.5 were observed in the Torino, Brescia and Pisa samples in winter. The Ames test showed low mutagenic activity. The highest net revertants/m3 were observed in the Torino and Brescia samples (winter), and the mutagenic effect was associated with PM0.5 (p < 0.01), PAH and nitro-PAH (p < 0.05) concentrations. The YG1021 strain showed the highest sensitivity to PM0.5 samples. No genotoxic effect of PM0.5 extracts was observed using A549 cells except for some samples in winter (comet assay), while BEAS-2B cells showed light DNA damage in the Torino, Brescia and Pisa samples in winter, highlighting the higher sensitivity of BEAS-2B cells, which was consistent with the Ames test (p < 0.01). The results obtained showed that it is important to further investigate the finest fractions of PM, which represent a relevant percentage of PM10, taking into account the chemical composition and the biological effects induced. Results highlighted the importance to further investigate the finest fractions of PM, which represent a relevant percentage of PM10, taking into account its chemical composition and the biological effects induced.

Published
2019

5. Results from the European Union MAPEC_LIFE cohort study on air pollution and chromosomal damage in children: are public health policies sufficiently protective?

Author

Ceretti, E., Donato, F., Zani, C., Villarini, M., Verani, M., De Donno, A., Bonetta, S., Feretti, D., Carducci, A., Idolo, A., Carraro, E., Covolo, L., Moretti, M., Palomba, G., Grassi, T., Bonetti, A., Bonizzoni, S., Biggeri, A., Gelatti, U., Festa, A., Viola, G. C. V., Zerbini, I., Fatigoni, C., Levorato, S., Monarca, S., Salvatori, T., Vannini, S., Donzelli, G., Bagordo, F., De Giorgi, M., Guido, M., Panico, A., Serio, F., Tumolo, M. R., Gea, M., Gilli, G., Pignata, C., Schiliro, T., Romanazzi, V., Furia, C., Bruni, B., and Casini, B.

Subjects
medicine.medical_specialty, Buccal swab, Air pollution, 010501 environmental sciences, medicine.disease_cause, 01 natural sciences, 03 medical and health sciences, symbols.namesake, 0302 clinical medicine, Environmental health, Epidemiology, medicine, media_common.cataloged_instance, Air quality policy, 030212 general & internal medicine, Poisson regression, European union, Air pollution exposure, Biomarkers of early effects, Children, Chromosomal damage, Air quality index, 0105 earth and related environmental sciences, media_common, Pollutant, business.industry, Pollution, symbols, business, Cohort study
Abstract

Following publication of the original article [1], the typesetters have missed to add the below listed study group author names in XML in author group section. The study group authors have been added to the author group and are presented correctly in this correction article. A minor change has been made to the electronic supplementary files by removing the yellow highlights and included in this correction. Study Group Authors: Andrea Festa1 Gaia Claudia Viviana Viola1 Ilaria Zerbini1 Cristina Fatigoni2 Sara Levorato2 Silvano Monarca2 Tania Salvatori2 Samuele Vannini2 Gabriele Donzelli3 Francesco Bagordo4 Mattia De Giorgi4 Marcello Guido4 Alessandra Panico4 Francesca Serio4 Maria Rosaria Tumolo4 Silvia Bonetta5 Marta Gea5 Giorgio Gilli5 Cristina Pignata5 Tiziana Schilirò5 Valeria Romanazzi5 Camilla Furia6 Beatrice Bruni7 Beatrice Casini7 Study Group Author Affiliations: 1Department of Medical and Surgical Specialties, Radiological Science and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy 2Department of Pharmaceutical Sciences, Unit of Public Health, University of Perugia, Via del Giochetto, 06122 Perugia, Italy 3Department of Biology, University of Pisa, 35/39 Via S. Zeno, 56127 Pisa, Italy 4Department of Biological and Environmental Science and Technology, University of Salento, 165 Via Monteroni, 73100 Lecce, Italy 5Department of Public Health and Pediatrics, University of Torino, 94 Piazza Polonia, 10126 Turin, Italy 6Brescia Municipality, 1 Piazza Repubblica, 25100 Brescia, Italy 7Department of Translational Research, N.T.M.S., University of Pisa, 35/39 Via S. Zeno, 56127 Pisa, Italy Author details 1 Department of Medical and Surgical Specialties, Radiological Science and Public Health, University of Brescia, 11 Viale Europa, 25123 Brescia, Italy. 2 Department of Pharmaceutical Sciences, Unit of Public Health, University of Perugia, Via del Giochetto, 06122 Perugia, Italy. 3 Department of Biology, University of Pisa, 35/39 Via S. Zeno, 56127 Pisa, Italy. 4 Department of Biological and Environmental Science and Technology, University of Salento, 165 Via Monteroni, 73100 Lecce, Italy. 5 Department of Public Health and Pediatrics, University of Torino, 94 Piazza Polonia, 10126 Turin, Italy. 6 Multi-sector and Technological Service Centre - CSMT Gestione S.c.a.r.l, 45 Via Branze, 25123 Brescia, Italy. 7 Brescia Municipality, 1 Piazza Repubblica, 25100 Brescia, Italy. 8 Department of Statistic, Computer Science, Applications, University of Florence, 59 Viale Morgagni, 50134 Florence, Italy.

Published
2020

6. Correction to: Results from the European Union MAPEC_LIFE cohort study on air pollution and chromosomal damage in children: are public health policies sufficiently protective? (Environmental Sciences Europe, (2020), 32, 1, (74), 10.1186/s12302-020-00352-3)

Author

Ceretti, E., Donato, F., Zani, C., Villarini, M., Verani, M., De Donno, A., Bonetta, S., Feretti, D., Carducci, A., Idolo, A., Carraro, E., Covolo, L., Moretti, M., Palomba, G., Grassi, T., Bonetti, A., Bonizzoni, S., Biggeri, A., Gelatti, U., Festa, A., Viola, G. C. V., Zerbini, I., Fatigoni, C., Levorato, S., Monarca, S., Salvatori, T., Vannini, S., Donzelli, G., Bagordo, F., De Giorgi, M., Guido, M., Panico, A., Serio, F., Tumolo, M. R., Gea, M., Gilli, G., Pignata, C., Schiliro, T., Romanazzi, V., Furia, C., Bruni, B., and Casini, B.

Subjects
air pollution, chromosomal damage, public health policies, air pollution, public health policies, chromosomal damage
Published
2020

12. Mutagenic and genotoxic effects induced by PM0.5 of different Italian towns in human cells and bacteria: The MAPEC_LIFE study

Author

Bonetta, Sara, primary, Bonetta, Silvia, additional, Schilirò, Tiziana, additional, Ceretti, Elisabetta, additional, Feretti, Donatella, additional, Covolo, Loredana, additional, Vannini, Samuele, additional, Villarini, Milena, additional, Moretti, Massimo, additional, Verani, Marco, additional, Carducci, Annalaura, additional, Bagordo, Francesco, additional, De Donno, Antonella, additional, Bonizzoni, Silvia, additional, Bonetti, Alberto, additional, Pignata, Cristina, additional, Carraro, Elisabetta, additional, Gelatti, Umberto, additional, Gilli, G., additional, Romanazzi, V., additional, Gea, M., additional, Festa, A., additional, Viola, G.C.V., additional, Zani, C., additional, Zerbini, I., additional, Donato, F., additional, Monarca, S., additional, Fatigoni, C., additional, Levorato, S., additional, Salvatori, T., additional, Donzelli, G., additional, Palomba, G., additional, Casini, B., additional, De Giorgi, M., additional, Devoti, G., additional, Grassi, T., additional, Idolo, A., additional, Panico, A., additional, Serio, F., additional, Furia, C., additional, and Colombi, P., additional

Published
2019
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13. Are 6-8 year old Italian children moving away from the Mediterranean diet?

Author

Zani, Claudia, Ceretti, Elisabetta, Grioni, S., Viola, Gaia Claudia Viviana, Donato, Francesco, Feretti, Donatella, Festa, Andrea, Bonizzoni, S., Bonetti, A., Monarca, S., Villarini, M., Levorato, S., Carducci, A., Verani, M., Casini, B., De Donno, A., Grassi, T., Bagordo, F., Carraro, E., Bonetta, S. i., Bonetta, S. a., Gelatti, Umberto, Covolo, Loredana, Levaggi, Rosella, Limina, Rosa Maria, Mazzoleni, Giovanna, Zerbini, Ilaria, Furia, C., Colombi, P., Braga, F., Fatigoni, C., Moretti, M., Vannini, S., Bruni, B., Caponi, E., Donzelli, G., Idolo, A., Guido, M., Devoti, G., Tumolo, M. R., Serio, F., Romanazzi, V., Pignata, C., Schilirò, T., Gilli, G., Zani, C, Ceretti, E, Grioni, S, Viola, G. C. V, Donato, F, Feretti, D, Festa, A, Bonizzoni, S, Bonetti, A, Monarca, S, Villarini, M, Levorato, S, Carducci, A, Verani, M, Casini, B, DE DONNO, Maria Antonella, Grassi, Tiziana, Bagordo, Francesco, Carraro, E, Bonetta, Si, Bonetta, Sa, Gelatti, U, Covolo, L., Levaggi, R., Limina, R. M., Mazzoleni, G., Zerbini, I., Furia, C., Colombi, P., Braga, F., Fatigoni, C., Moretti, M., Vannini, S., Bruni, B., Caponi, E., Donzelli, G., Idolo, Adele, Guido, Marcello, Devoti, G., Tumolo, M. R., Serio, Francesca, Romanazzi, V., Pignata, C., Schilirò, T., and Gilli, G.

Subjects
Medicine (all), Mediterranean diet, Food-frequency questionnaire, Italian mediterranean index, Food-frequency questionnaire, Mediterranean diet, Italian Mediterranean Index, Italian Mediterranean Index
Abstract

The Mediterranean diet (MD) is considered one of the healthiest dietary models, as it decreases the risk of chronic diseases and may modulate the organism's early response to environmental pollution. In recent decades, Mediterranean countries have been replacing their traditional diet with other less healthy eating habits, especially among children and teenagers.

Published
2016

14. monitoring air pollution effects on children for supporting public health policy: the protocol of the prospective cohort MAPEC study

Author

Feretti, D., Ceretti, E., Moretti, M., Carducci, A., Bonetta, S., Marrese, M. R., Bonetti, A., Covolo, L., Villarini, M., Verani, M., Schilirò, T., Limina, R. M., Monarca, S., Casini, B., Carraro, E., Zani, C., Mazzoleni, G., Levaggi, R., Gelatti, U., Compiani, S., Donato, F., Festa, A., Viola, G. C. V., Zerbini, I., Idolo, A., Serio, F., Tumolo, M. R., Dominici, L., Fatigoni, C., Levorato, S., Peverini, M., Vannini, S., Bruni, B., Caponi, E., Donzelli, G., Gilli, G., Pignata, C., Bonizzoni, S., Furia, C., Braga, F., Codenotti, R., Colombi, P., Lini, D., Mario, E., DE DONNO, Maria Antonella, BAGORDO, Francesco, GRASSI, Tiziana, GUIDO, Marcello, VERRI, Tiziano, Feretti, D., Ceretti, E., DE DONNO, Maria Antonella, Moretti, M., Carducci, A., Bonetta, S., Marrese, M. R., Bonetti, A., Covolo, L., Bagordo, Francesco, Villarini, M., Verani, M., Schilirò, T., Limina, R. M., Grassi, Tiziana, Monarca, S., Casini, B., Carraro, E., Zani, C., Mazzoleni, G., Levaggi, R., Gelatti, U., Compiani, S., Donato, F., Festa, A., Viola, G. C. V., Zerbini, I., Guido, Marcello, Idolo, A., Serio, F., Tumolo, M. R., Verri, Tiziano, Dominici, L., Fatigoni, C., Levorato, S., Peverini, M., Vannini, S., Bruni, B., Caponi, E., Donzelli, G., Gilli, G., Pignata, C., Bonizzoni, S., Furia, C., Braga, F., Codenotti, R., Colombi, P., Lini, D., Mario, E., Feretti, D, Ceretti, E, Moretti, M, Carducci, A, Bonetta, S, Marrese, M. R, Bonetti, A, Covolo, L, Villarini, M, Verani, M, Schilirò, T, Limina, R. M, Monarca, S, Casini, B, Carraro, E, Zani, C, Mazzoleni, G, and Levaggi, R

Subjects
COMET ASSAY, air pollution, Air pollution, SALMONELLA MUTAGENICITY, environmental pollution, Public health, child health, Mutagenesis, mucosa buccal cell, medicine.disease_cause, MICRONUCLEUS FREQUENCY, Epidemiology, Environmental monitoring, Protocol, Prospective Studies, Child, ENVIRONMENTAL-POLLUTANTS, Health Policy, Absolute risk reduction, General Medicine, PERIPHERAL-BLOOD LYMPHOCYTES, Public Health, mucosa buccal cells, Human, Environmental Monitoring, medicine.medical_specialty, EUROPEAN COHORTS, AMBIENT AIR, DNA damage, PUBLIC HEALTH, children, early biological effect, Air Pollution, Humans, Medicine (all), Environmental health, medicine, EXPOSURE, Socioeconomic status, Health policy, Pollutant, GENETIC-DAMAGE, business.industry, IN-VITRO, Prospective Studie, business
Abstract

Introduction Genotoxic biomarkers have been studied largely in adult population, but few studies so far have investigated them in children exposed to air pollution. Children are a high-risk group as regards the health effects of air pollution and some studies suggest that early exposure during childhood can play an important role in the development of chronic diseases in adulthood. The objective of the project is to evaluate the associations between the concentration of urban air pollutants and biomarkers of early biological effect in children, and to propose a model for estimating the global risk of early biological effects due to air pollutants and other factors in children. Methods and analysis Two biomarkers of early biological effects, DNA damage by the comet assay and the micronuclei (MN) test, will be investigated in oral mucosa cells of 6–8-year-old children. Concurrently, some toxic airborne pollutants (polycyclic aromatic hydrocarbon (PAH) and nitro-PAH) and in vitro air mutagenicity and toxicity in ultra-fine air particulates (PM0.5) will be evaluated. Furthermore, demographic and socioeconomic variables, other sources of exposures to air pollutants and lifestyle variables will be assessed by a structured questionnaire. The associations between sociodemographic, environmental and other exposure variables and biomarkers of early biological effect using univariate and multivariate models will be analysed. A tentative model for calculating the global absolute risk of having early biological effects caused by air pollution and other variables will be proposed. Ethics and dissemination The project has been approved by the Ethics Committees of the local Health Authorities. The results will be communicated to local Public Health Agencies, for supporting educational programmes and health policy strategies. LIFE+2012 Environment Policy and Governance. LIFE12 ENV/IT/000614.

Published
2014

16. Feasibility and reliability of a questionnaire for evaluation of the exposure to indoor and outdoor air pollutants, diet and physical activity in 6-8-year-old children

Author

Zani, C, Donato, F, Grioni, S, Viola, G. C. V, Ceretti, E, Feretti, D, Festa, A, Bonizzoni, S, Bonetti, A, Monarca, S, Villarini, M, Levorato, S, Carducci, A, Verani, M, Casini, B, Carraro, E, Gilli, G, Bonetta, S, Gelatti, U, Covolo, L, Levaggi, R, Limina, RM, Mazzoleni, G, Zerbini, I, Furia, C, Marrese, MR, Colombi, P, Mario, E, Braga, F, Fatigoni, C, Moretti, M, Vannini, S, Bruni, B, Caponi, E, Donzelli, G, Tumolo, MR, Romanazzi, V, Pignata, C, Schilirò T., DE DONNO, Maria Antonella, GRASSI, Tiziana, IDOLO, ADELE, BAGORDO, Francesco, GUIDO, Marcello, DEVOTI, Gabriele, SERIO, FRANCESCA, Zani, C, Donato, F, Grioni, S, Viola, G. C. V, Ceretti, E, Feretti, D, Festa, A, Bonizzoni, S, Bonetti, A, Monarca, S, Villarini, M, Levorato, S, Carducci, A, Verani, M, Casini, B, DE DONNO, Maria Antonella, Grassi, Tiziana, Idolo, Adele, Carraro, E, Gilli, G, Bonetta, S, Gelatti, U, Covolo, L, Levaggi, R, Limina, Rm, Mazzoleni, G, Zerbini, I, Furia, C, Marrese, Mr, Colombi, P, Mario, E, Braga, F, Fatigoni, C, Moretti, M, Vannini, S, Bruni, B, Caponi, E, Donzelli, G, Bagordo, Francesco, Guido, Marcello, Devoti, Gabriele, Tumolo, Mr, Serio, Francesca, Romanazzi, V, Pignata, C, and Schilirò, T.

Subjects
Male, Reproducibility of Result, Environmental Exposure, Health Survey, Motor Activity, Reliability, Food-frequency questionnaire, Diet, Health Statu, Feasibility Studie, Italy, Parent, Air Pollution, Indoor, Surveys and Questionnaires, Female, Repeatability, Child, Children, Environmental Monitoring, Human
Abstract

The MAPEC-Life project aims to study the biological effects of early exposure to air pollutants on the oral mucosa cells of school-age children in five Italian cities. A questionnaire was created to evaluate the association between outdoor and indoor airborne pollutants, lifestyle, diet and biomarker effects. The feasibility and reliability of the questionnaire were evaluated.

Published
2015

17. Raising awareness of children on air pollution and its health effects: the MAPEC_LIFE experience in health communication

Author

annalaura carducci, BEATRICE CASINI, Ceretti, E., Zani, C., MARCO VERANI, Donno Ma, A., Grassi, T., Carraro, E., Bonetta, S., Monarca, S., Bonizzoni, S., Bonetti, A., Gelatti, U., Carducci, A, Casini, B, Ceretti, E, Zani, C, Verani, M, DE DONNO, Maria Antonella, Grassi, Tiziana, Carraro, E, Bonetta, Sa, Monarca, S, Bonizzoni, S, Bonetti, A, and Gelatti, U.

Published
2015

25. Exposure to air pollution and lifestyles of children partecipating in the MAPEC_LIFE (Monitoring Air Pollution Effects on Children for Supporting Public Health Policy) study

Author

Grassi, T, DONNO A, De, Bagordo, F, Ceretti, E, Zani, C, Moretti, M, Levorato, S, Carducci, A, Verani, M, Bonetta, S, Bonizzoni, S, Bonetti, A, Guido, M, Devoti, G, Gelatti, U, Bruni, B, Carraro, E, Casini, B, Codenotti, R, Colombi, T, Covolo, L, Crottini, S, De Giorgi, M, Donato, F, Donzelli, G, Fatigoni, C, Feretti, D, Festa, A, Furia, C, Gaffurini, L, Gilli, G, Idolo, A, Limina, Rm, Monarca, S, Pignata, C, Romanazzi, V, Salvatori, T, Schilirò, T, Serio, F, Vannini, S, Verri, T, Villarini, M, Viola, Gcv, Zagni, L, and Zerbini, I

Published
2016

26. Are 6-8 year old Italian children moving away from the Mediterranean diet?

Author

Zani, C., Ceretti, E., Grioni, S., Viola, G. C. V., Donato, F., Feretti, D., Festa, A., Bonizzoni, S., Bonetti, A., Monarca, S., Villarini, M., Levorato, S., Carducci, A., Verani, M., Casini, B., De Donno, A., Grassi, T., Idolo, A., Carraro, Elisabetta, Bonetta, Silvia, Bonetta, Sara, Gelatti, MAPEC LIFE Study Group, U., Romanazzi, Valeria, Pignata, Cristina, Schiliro', Tiziana, and Gilli, Giorgio

Subjects
Mediterranean diet, Food-frequency questionnaire, Mediterranean diet, Italian Mediterranean Index, Italian Mediterranean Index, Food-frequency questionnaire
Published
2016

27. The MAPEC_LIFE Study: indoor/outdoor air pollution exposure and lifestyles of the prospective cohort

Author

DONNO A, De, Bagordo, F, Zani, C, Ceretti, E, Villarini, M, Vannini, S, Carducci, A, Casini, B, Bonetta, S, Schilirò, T, Bonizzoni, S, Bonetti, A, Grassi, T, Guido, M, Devoti, G, Gelatti, U, Serio, F., Idolo, A., De Giorgi, M., Tumolo, M. R., Verri, T., Covolo, L., Donato, F., Feretti, D., Festa, A., Limina, R. M., Viola, G. C., Zerbini, I., Fatigoni, C., Levorato, S., Monarca, S., Moretti, M., Salvatori, T., Donzelli, G., Verani, M., Bruni, B., Bonetta, Si., Carraro, E., Gilli, G., Pignata, C., Romanazzi, V., Furia, C., Codenotti, R., Colombi, P., Crottini, S., Gaffurini, L., and Zagni, L.

Published
2016

28. A pilot environmental education project in the MAPEC study

Author

Pezzola, Deborha, Zani, Claudia, Ceretti, Elisabetta, Bonizzoni, S, Bonetti, A, Monarca, S, Carducci, A, Casini, B, DE DONNO, Ma, Carraro, E, Gelatti, Umberto, and Group, MAPEC LIFE study

Subjects
Environmental health
Published
2015

29. Feasibility and reliability of a questionnaire for evaluation of the exposure to indoor and outdoor air pollutants, diet and physical activity in 6-8-year-old children

Author

Zani, C., Donato, F., Grioni, S., Viola, G. C. V., Ceretti, E., Feretti, D., Festa, A., Bonizzoni, S., Bonetti, A., Monarca, S., Villarini, M., Levorato, S., Carducci, A., Verani, M., Casini, B., De Donno, M. A., Grassi, T., Idolo, A., Carraro, Elisabetta, Gilli, Giorgio, Bonetta, Sara, Gelatti, MAPEC LIFE study Group, U., Bonetta, Silvia, Romanazzi, Valeria, Pignata, Cristina, and Schiliro', Tiziana

Subjects
Male, Parents, food-frequency questionnaire, repeatability, reliability, children, Health Status, Children, Food-frequency questionnaire, Reliability, Repeatability, Reproducibility of Results, Children, Food-frequency questionnaire, Reliability, Repeatability, Environmental Exposure, Motor Activity, Health Surveys, Diet, Italy, Air Pollution, Indoor, Surveys and Questionnaires, Feasibility Studies, Humans, Female, Child, Environmental Monitoring
Abstract

The MAPEC-Life project aims to study the biological effects of early exposure to air pollutants on the oral mucosa cells of school-age children in five Italian cities. A questionnaire was created to evaluate the association between outdoor and indoor airborne pollutants, lifestyle, diet and biomarker effects. The feasibility and reliability of the questionnaire were evaluated.A questionnaire was drawn up to be filled in by the parents of 6-8-year-old children. It consisted of 148 questions on the children's health, physical activity, environmental exposures and the frequency of food consumption at the main meals. First we conducted a questionnaire feasibility study involving 53 volunteer parents. We then performed a reliability study by administering the questionnaire to a further 156 parents and again one month later (test/retest method). The correlations between answers at the first and second administration of the questionnaire were evaluated using the Kappa statistic and Spearman's coefficient.After verifying the feasibility of the questionnaire, we conducted a reliability analysis on 132 completed questionnaires. The percentage of agreement between the first and the second responses given was over 70%, all K values being greater than 0.6. The analysis of calories and macronutrients also showed good agreement.The questionnaire drawn up for the study proved to be sufficiently reliable for gathering information about the factors of interest in our study of the relationship between air pollution and early biological effects in children.

Published
2015

31. Monitoring air pollution effects in children for supporting public health policy: the MAPEC_LIFE study

Author

Ceretti, Elisabetta, Bonizzoni, S, Bonetti, A, Monarca, S, Carducci, A, DONNO MA, De, Carraro, E, Gelatti, Umberto, MAPEC_LIFE STUDY GROUP, The, Covolo, Loredana, Donato, Francesco, Feretti, Donatella, Festa, Andrea, Limina, Rosa Maria, Viola, Gaia Claudia Viviana, Zani, Claudia, Zerbini, Ilaria, Levaggi, Rosella, Mazzoleni, Giovanna, and Furia, C.

Subjects
Environmental health
Published
2014

33. The MAPEC_LIFE study (LIFE12 ENV/IT/000614): monitoring air pollution effects in children for supporting public health policy

Author

MARCO VERANI, annalaura carducci, BEATRICE CASINI, Marrese, M. R., Bonetti, A., Monarca, S., Donno, M. A., Carraro, E., Gelatti, U., Caponi, Elisa, Donzelli, Gabriele, Bruni, Beatrice, Ceretti, E., Covolo, L., Donato, F., Feretti, D., Festa, A., Limina, R. M., Viola, G. C. V., Zani, C., Zerbini, I., Levaggi, R., Mazzoleni, G., Bonizzoni, S., Furia, C., Codenotti, R., Colombi, P., Crottini, S., Mario, E., Dominici, L., Fatigoni, C., Levorato, S., Moretti, M., Vannini, S., Villarini, M., Bagordo, F., Grassi, T., Guido, M., and Idolo, A.

Published
2014

34. Monitoraggio degli effetti dell’inquinamento atmosferico sui bambini a sostegno delle strategie di sanità pubblica: il progetto MAPEC_LIFE

Author

Donato, Francesco, Bonizzoni, S., Bonetti, A., Monarca, S., Carducci, A., DE DONNO MA, Carraro, E., Gelatti, Umberto, Elisabetta Ceretti, Loredana Covolo, Feretti, Donatella, Festa, Andrea, Rosella Levaggi, Limina, Rosa Maria, Giovanna Mazzoleni, Viola, G. C. V., Claudia Zani, Furia, I. Zerbini C., Codenotti, M. R. Marrese R., Colombi, P., Crottini, S., Dominici, E. Mario L., Fatigoni, C., Levorato, S., Moretti, M., Vannini, S., Bruni, M. Villarini B., Caponi, E., Casini, B., Bagordo, M. Verani F., Grassi, T., Guido, M., Idolo, A., Bonetta, T. Verri S., Gilli, G., Pignata, C., and T. Schilirò .

Published
2014

35. La disinfezione della rete idrica nei riuniti odontoiatrici: risultati di uno studio multicentrico in Italia

Author

Montagna, M. T., Liguori, G., Badolati, G., Castiglia, P., Di Rosa, E., Gregorio, Pasquale, Monarca, S., Pasquarella, C., Sindoni, L., Stampi, S., Tanzi, M. L., Tarsitani, G., Montagna MT, Liguori G, Badolati G, Castiglia P, Di Rosa E, Gregorio P, Monarca S, Pasquarella C, Sindoni L, Stampi S, Tanzi ML, and Tarsitani G e Gruppo di lavoro SITI

Subjects
disinfezione, riuniti odontoiatrici, studio multicentrico
Published
2004

38. Gestione delle risorse idriche in italia

Author

Aggazzotti, Gabriella, Agodi, A, Carreri, V, Conversano, M, De Donno, A, Fallico, R, Faggioli, A, Ferrante, M, Francia, F, Monarca, S, Petronio, Mg, Sciacca, S, Signorelli, C, Vinceti, Marco, Olivieri Conti, G, Mauceri, C, Dimartino, A, Fiore, M, Ledda, C, Copat, C, Grasso, A, and Cafarella, N.

Subjects
risorse idriche, Italia, salute
Published
2013

39. Gestione delle Risorse Idriche in Italia

Author

Agazzotti, G, Agodi, ANTONELLA PAOLA, Carreri, V, Conversano, M, De Donno, A, Fallico, R, Faggioli, A, Ferrante, Margherita, Francia, F, Monarca, S, Petronio, Mg, Sciacca, S, Signorelli, C, Vinceti, M, Oliveri Conti, G, Mauceri, C, Dimartino, A, Fiore, Maria, Ledda, C, Copat, C, Grasso, A, and Cafarella, N.

Published
2013

42. The buccal micronucleus cytome assay as a tool for the evaluation of air pollution early biological effects in children: Current status of the MAPEC (Monitoring Air Pollution Effects on Children for supporting public health policy) study

Author

Levorato, S., primary, Vannini, S., additional, Ceretti, E., additional, Villarini, M., additional, Bonetta, S., additional, Fatigoni, C., additional, Carducci, A., additional, Moretti, M., additional, De Donno, M.A., additional, Monarca, S., additional, Bonizzoni, S., additional, Bonetti, A., additional, and Gelatti, U., additional

Published
2015
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46. Genotossicità di acque minerali in PET

Author

Feretti, Donatella, Ceretti, Elisabetta, Zerbini, Ilaria, Guzzella, L., Scaglia, M., Berna, V., Donato, Francesco, Monarca, S., and Zani, Claudia

Published
2010

50. [Assessment of the genotoxic effects of gaseous emissions and percolates from three municipal landfills using plant bioassays]

Author

Feretti D, Elisabetta Ceretti, Zerbini I, Rm, Limina, Dominici L, Moretti M, Monarca S, and Donato F

Subjects
Chromosome Aberrations, Air Pollutants, Mutagenicity Tests, Meristem, Chromosomes, Plant, Refuse Disposal, Italy, Tradescantia, Onions, Humans, Soil Pollutants, Biological Assay, Environmental Pollutants, Cities, Environmental Monitoring, Mutagens
Abstract

The biomonitoring of genotoxic effects in environmental complex mixtures using higher plants is very useful for hazard evaluation. In this study we evaluated the potential application of plant genotoxicity tests in monitoring mutagens in landfill environment. The clastogenic effects of gaseous emissions (biogas)from three municipal landfills were evaluated by in situ monitoring using the Tradescantia micronucleus assay. The cytotoxicity and genotoxicity of leachates were studied using the Allium cepa test. We found no significant differences in micronuclei frequency in pollen cells of Tradescantia. Leachate samples showed elevated toxicity that inhibited root tip development in Allium cepa. Genotoxicity of the leachates was evaluated in diluted samples only. We found a significant increase in chromosomal metaphase aberrations only in one of the samples analyzed. In conclusion, biogas was not shown to be a real hazard, whereas leachates were found to display elevated toxicity. It would be advisable to treat leachates before releasing them into the environment as they can cause ecological damages. Since plant bioassays are very useful for the in situ monitoring of environmental genotoxins they are important for the prevention of environmental pollution resulting from the disposal of solid waste.

Published
2009

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