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The study aimed to assess the environmental health impacts of the liquid waste from Assalaya Sugar Factory, the efficiency of the existing Assalaya effluent treatment plant, the dilution factors available in the White Nile to gather with wastewater environmental impacts. A descriptive cross-sectional focused on the Factory and its neighborhoods. Four hundred and thirty two out of 3931 households were statistically determined as the sample size, the individual samples were picked using multi-stage stratified method 432 households selected as sample size. Data were collected by using structured questionnaires, field observations, laboratory analysis and interviewing the concerned and affected persons. The effluent load discharged from the factory into the Al - jassir canal at the White Nile was analyzed for BOD, COD, pH, PO4, TDS, TSS, Turbidity, Color, and flow rate.The Data were processed by using the Statistical Package for Social Science (SPSS) version 16, Chi-square test, test associations and office excel 2007. The study showed that Eighty one percent of the households used the surplus irrigation canal as a source for water supply. 64% of the respondents suffered from diarrhea, vomiting and allergic diseases, the rather low rate of water consumption and the bad quality of water consumed were reflected adversely on hygiene and consequently increased water related diseases. The study concludes that always or sometime 49.5% of the water collectors were children and used animals and plastic containers for water collection and transportation. The conducted laboratory water analysis revealed that the average concentrations of PO4, COD and BOD of the raw wastewater produced by Assalaya Sugar Factory were 4260, 3800 and 1500 mg/l, respectively, these values were above the WHO recommended concentrations for the disposed treated effluent (2, 250 and 30 mg/L respectively). As to physical analysis; the turbidity on the average was higher (540 NTU) and the color was (854 TCU) also high.

The KatG gene of Mycobacterium Tuberculosis has been associated with isoniazid (INH) drug resistance. While isoniazid (INH) considered as corner stone and main chemotherapy used throughout the world to manage tuberculosis, thus the Progress in apprehension of principle concepts associated with resistance to isoniazid (INH) has allowed molecular tests in addition to bioinformatics tool for the detection of drug-resistant tuberculosis to be developed. In Consecutive isolates (n = 20) of multidrug-resistant Mycobacterium tuberculosis, part of the katG was sequenced for INH resistance analysis. BLAST analysis of all sequences revealed 100% identity with the available strain “EGY-K361” Mycobacterium tuberculosis with Accession No: KC49137.1 except 6 isolates :isolate1, 2, 4, 11, 15, and isolate 20 revealed 99% identity. Thosesix isolates (30%) have detected mutation in Catalase-peroxidase enzyme S315T; three isolate from six 3/6 (50%) of mutant isolates have SNP AGC>ACC substitution while others 3/6 have substitution C>G in position 1280 which may contributed in altering gene expression. The secondary structure of wild and mutant proteins had been done using phyre2 software while the three dimensional structures of them had been done by Chimera software. Stability of mutant protein was increased which detected by i-mutant. Phylogenetic tree of the sequences revealed two distinct phylogroups: mutant isolates and wild isolates phylogroups with controls from different countries retrieved from Gene bank. Serine at position 315 is one of potential drug active sites that proved via SiteEngine soft ware, therefore any substitution will change efficiency of INH.