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Breast cancer, a complex disease with a significant prevalence to form metastases, necessitates novel therapeutic strategies to improve treatment outcomes. Here, we present the results of a comparative molecular study of primary breast tumours, their metastases, and the corresponding primary cell lines using Desorption Electrospray Ionisation (DESI) and Laser-Assisted Rapid Evaporative Ionisation Mass Spectrometry (LA-REIMS) imaging. Our results show that ambient ionisation mass spectrometry technology is suitable for rapid characterisation of samples, providing a lipid- and metabolite-rich spectrum within seconds. Our study demonstrates that the lipidomic fingerprint of the primary tumour is not significantly distinguishable from that of its metastasis, in parallel with the similarity observed between their respective primary cell lines. While significant differences were observed between tumours and the corresponding cell lines, distinct lipidomic signatures and several phospholipids such as PA(36:2), PE(36:1), and PE(P-38:4)/PE(O-38:5) for LA-REIMS imaging and PE(P-38:4)/PE(O-38:5), PS(36:1), and PI(38:4) for DESI-MSI were identified in both tumours and cells. We show that the tumours' characteristics can be found in the corresponding primary cell lines, offering a promising avenue for assessing tumour responsiveness to therapeutic interventions. A comparative analysis by DESI-MSI and LA-REIMS imaging revealed complementary information, demonstrating the utility of LA-REIMS in the molecular imaging of cancer. [ABSTRACT FROM AUTHOR]

This paper is concerned with the role of network administrative organizations (NAOs) in the development of social capital in inter-organizational networks aiming at supporting their members to innovate in the food sector through interacting with one another. A multi-case study approach is used whereby three Belgian inter-organizational networks are investigated i.e. Wagralim, Réseau-Club and Flanders Food. Our study shows that there are many options available to NAOs to build social capital within the networks they are responsible for; options which we propose to categorize in three main distinct groups: creation of boundary objects, careful selection of members and effective communication. [ABSTRACT FROM AUTHOR]

This study examines traditional food chain goals and explores the match with general consumer perceptions and preferences in relation to traditional food as a food product category. Chain goals were selected using focus group discussions and in-depth interviews (84 chain members, three European countries, five traditional food categories), as well as a literature review and a small scale survey (26 chain members, three European countries, three traditional food categories). Consumer data was gathered in six European countries via qualitative focus group discussions (95 participants), word association tests (721 participants), and a quantitative survey (4,828 participants). The study identifies five key chain goals: 'To maintain traditionalism,' 'To increase efficiency,' 'To improve responsiveness,' 'To improve quality,' and 'To create chain balance.' Traditionalism and quality goals were relevant and important to consumers, whereas efficiency, responsiveness, and chain balance goals were not directly recognized by or apparent to them. [EconLit citations: D12; D74; L14]. © 2010 Wiley Periodicals, Inc. [ABSTRACT FROM AUTHOR]

Organizations no longer compete as independent entities, but as chains (Christopher, 1998; Cox, 1999; Lambert, Cooper, 2000). Consequently, chain strategies became more important in creating competitive advantage (Vickery et al., 2003; Gunasekaran et al., 2004). Despite the growing recognition of the importance of chain strategies, many chains active in the agri-business sector still face difficulties in developing common chain strategies and implementing them collaboratively to generate additional mutual gains and savings. Chains lacking a chain strategy and having short-term perspectives face difficulties in envisaging and implementing cooperative solutions to problems they cannot manage alone. Despite this recognition, the actual development of such chain strategies lags behind because of some particular issues which still need to be addressed (e.g. vision, mission, values or action plans). Therefore, the objective of this paper is to identify and consolidate chain members' goals, to select a minimum set of key goals and to confront these chain goals with consumer preferences. This way, the paper develops the vision of traditional food chains in the EU, as a first step of strategy development. First, chain members goals are identified and consolidated with the help of approximately 100 stakeholders (suppliers, focal companies, customers) from three European countries representing 5 traditional food product categories (cheese, beer, dry ham, dry sausage and white pepper). The most important goals of traditional food chains are to maintain traditionalism, to improve responsiveness, to maintain superior quality and to create chain balance. These chain goals are tested against the perception of 4828 consumers from six European countries (Belgium, France, Italy, Norway, Poland and Spain). As a result, the vision of traditional food chains is developed: the European traditional food sector envisions a competitive sector maintaining the traditional character and superior quality of their food product(s) via more efficient and responsive chains where risks and benefits are distributed in a balanced way between the members. [ABSTRACT FROM AUTHOR]

Introduction: Lipid nanovesicles associated with bioactive phytochemicals from spruce needle homogenate (here called nano-sized hybridosomes or nanohybridosomes, NSHs) were considered., Methods: We formed NSHs by mixing appropriate amounts of lecithin, glycerol and supernatant of isolation of extracellular vesicles from spruce needle homogenate. We visualized NSHs by light microscopy and cryogenic transmission electron microscopy and assessed them by flow cytometry, dynamic light scattering, ultraviolet-visual spectroscopy, interferometric light microscopy and liquid chromatography-mass spectrometry., Results: We found that the particles consisted of a bilayer membrane and a fluid-like interior. Flow cytometry and interferometric light microscopy measurements showed that the majority of the particles were nano-sized. Dynamic light scattering and interferometric light microscopy measurements agreed well on the average hydrodynamic radius of the particles R h (between 140 and 180 nm), while the concentrations of the particles were in the range between 10 13 and 10 14 /mL indicating that NSHs present a considerable (more than 25%) of the sample which is much more than the yield of natural extracellular vesicles (EVs) from spruce needle homogenate (estimated less than 1%). Spruce specific lipids and proteins were found in hybridosomes., Discussion: Simple and low-cost preparation method, non-demanding saving process and efficient formation procedure suggest that large-scale production of NSHs from lipids and spruce needle homogenate is feasible., Competing Interests: The authors report no conflicts of interest in this work., (© 2024 Spasovski et al.)