35 results on '"Moja, S."'
Search Results
2. Hybridization in the section Mentha (Lamiaceae) inferred from AFLP markers
- Author
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Gobert, V., Moja, S., Colson, M., and Taberlet, P.
- Subjects
Biological sciences - Abstract
The amplified fragment length polymorphism (AFLP) method was used to evaluate genetic diversity and to assess genetic relationships within the section Mentha in order to clarify the taxonomy of several interspecific mint hybrids with molecular markers. To this end, genetic diversity of 62 Mentha accessions from different geographic origins, representing five species and three hybrids, was assessed. Three EcoRI/MseI AFLP primer combinations generated an average of 40 AFLP markers per primer combination, ranging in size from 50 to 500 base pairs (bp). The percentage of markers polymorphic ranged from 50% to 60% across all accessions studied. According to phenetic and cladistic analysis, the 62 mint accessions were grouped into two major clusters. Principal coordinates analysis separated species into well-defined groups, and clear relationships between species and hybrids could be described. Our AFLP analysis supports taxonomic classification established among Mentha species by conventional (morphological, cytological, and chemical) methods. It allows the assessment of phenetic relationships between species and the hybrids M. spicata and M. x piperita, largely cultivated all over the world for their menthol source, and provides new insights into the subdivision of M. spicata, based for the first time on molecular markers. Key words: AFLP; DNA fingerprinting; genetic diversity; hybridization; Lamiaceae; Mentha; polyploid.
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- 2002
3. Agrobacterium tumefaciens-mediated transformation of Mentha spicata and Mentha arvensis
- Author
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Diemer, F., Caissard, J.C., Moja, S., and Jullien, F.
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- 1999
- Full Text
- View/download PDF
4. Plant regeneration and transient GUS expression in a range of lavandin (Lavandula × intermedia Emeric ex Loiseleur) cultivars
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Dronne, S., Colson, M., Moja, S., and Faure, O.
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- 1998
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5. Mannitol and thidiazuron improve in vitro shoot regeneration from spearmint and peppermint leaf disks
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Faure, O., Diemer, F., Moja, S., and Jullien, F.
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- 1998
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6. Rose floral scent
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Baudino, S., primary, Sun, P., additional, Caissard, J.-C., additional, Nairaud, B., additional, Moja, S., additional, Magnard, J.-L., additional, Bony, A., additional, Jullien, F., additional, Schuurink, R.C., additional, Vergne, P., additional, Dubois, A., additional, Raymond, O., additional, Bendahmane, M., additional, Hibrand-Saint Oyant, L., additional, Jeauffre, J., additional, Clotault, J., additional, Thouroude, T., additional, Foucher, F., additional, and Blerot, B., additional
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- 2019
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7. Amélioration des stratégies de lutte contre le dépérissement de la lavande et du lavandin
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CHAISSE, Eric, FOISSAC, Xavier, VERDIN, Eric, NICOLÈ, F., BOUVERAT-BERNIER, J.P., JAGOUEIX-EVEILLARD, Sandrine, SEMETEY, O., GAUDIN, Jonathan, FONTAINE, Marie, DANET, Jean-Luc, MOJA, S., CONORD, C., JULLIEN, F., LEGENDRE, L., GALLOIS, P., Centre Régionalisé Interprofessionnel d'Expérimentation en Plantes à Parfum, Aromatiques et Médicinales (CRIEPPAM), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Université Jean Monnet [Saint-Étienne] (UJM), Institut Technique Interprofessionnel des Plantes à Parfum Médicinales et Aromatiques (ITEIPMAI), Comité Interprofessionnel Huiles Essentielles Françaises, and Partenaires INRAE
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lavande ,[SDV]Life Sciences [q-bio] ,lavandin ,dépérissement ,tolérance ,Hyalesthes obsoletus ,phytoplasme du stolbur ,épidémiologie ,diagnostic ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology - Abstract
Textes issus des travaux du programme Casdar "Innovation et Partenariat" de 2007 et présentés lors d'un colloque le 4 décembre 2012, sous l'égide du GIS Relance Agronomique; National audience; Lavander and lavandin are symbolic crops in south-eastern France, in the region of Haute-Provence, but they have been affected by yellow decline for decades. The etiological agent which is involved in this decline is the stolbur phytoplasm, which is transmitted by the cixiidae Hyalesthes obsoletus. A reliable diagnosis has been developed, and permitted to secure the specification of phytosanitary certified material, according to the specifications “healthy plants”. A large epidemiological survey identified 16 genotypes of the stolbur phytoplasm. The study demonstrated that the epidemics mainly propagated from lavander to lavander. Finally, the study of the mechanism of tolerance showed that lavander and lavandin susceptibility was not correlated with the relation plant - phytoplasm, but more likely with the interaction of the insect with the plant. The insect is present in all cultivated plots, but in very variable densities. AFLP genotyping has also shown that genetic diversity of wild lavanders was quite restricted, and that genetic selection reduced it only a little.; La lavande et le lavandin sont des cultures emblématiques de Haute Provence, victimes d’un dépérissement depuis quelques décennies. L’agent étiologique de ce dépérissement est le phytoplasme du stolbur, et le principal vecteur de ce phytoplasme est la cicadelle Hyalesthes obsoletus. Une technique de diagnostic sensible et fiable a été mise au point, et a permis de sécuriser la certification du matériel végétal produit selon le cahier des charges plants sains. Une étude épidémiologique de grande envergure a montré que les parcelles étaient infectées par 16 génotypes différents du phytoplasme du stolbur, et que la maladie se propageait surtout de lavande(in) à lavande(in), et de lavanderaies à lavanderaies. Enfin, l’étude du phénomène de tolérance a montré que la sensibilité au dépérissement ne serait pas liée à la relation plante-phytoplasme, mais plus vraisemblablement à l’interaction de la cicadelle avec les plantes. Cette cicadelle est présente dans toutes les parcelles cultivées, mais à des effectifs très variables. Un génotypage AFLP a également montré que la diversité génétique des lavandes sauvages était assez restreinte, et que la sélection génétique effectuée par la filière l’avait très légèrement réduite.
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- 2012
8. Amélioration des stratégies de lutte contre le dépérissement de la lavande et du lavandin
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Eric Chaisse, Xavier Foissac, Eric Verdin, Nicolè, F., Bouverat-Bernier, J. P., Sandrine Jagoueix-Eveillard, Semetey, O., Jonathan Gaudin, Marie Fontaine, Jean-Luc Danet, Moja, S., Conord, C., Jullien, F., Legendre, L., Gallois, P., Centre Régionalisé Interprofessionnel d'Expérimentation en Plantes à Parfum, Aromatiques et Médicinales (CRIEPPAM), Biologie du fruit et pathologie (BFP), Université Bordeaux Segalen - Bordeaux 2-Institut National de la Recherche Agronomique (INRA)-Université Sciences et Technologies - Bordeaux 1, Unité de Pathologie Végétale (PV), Institut National de la Recherche Agronomique (INRA), Université Jean Monnet [Saint-Étienne] (UJM), Institut Technique Interprofessionnel des Plantes à Parfum Médicinales et Aromatiques (ITEIPMAI), Comité Interprofessionnel Huiles Essentielles Françaises, and Partenaires INRAE
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lavande ,[SDV]Life Sciences [q-bio] ,lavandin ,dépérissement ,tolérance ,Hyalesthes obsoletus ,phytoplasme du stolbur ,épidémiologie ,diagnostic ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology - Abstract
Textes issus des travaux du programme Casdar "Innovation et Partenariat" de 2007 et présentés lors d'un colloque le 4 décembre 2012, sous l'égide du GIS Relance Agronomique; National audience; Lavander and lavandin are symbolic crops in south-eastern France, in the region of Haute-Provence, but they have been affected by yellow decline for decades. The etiological agent which is involved in this decline is the stolbur phytoplasm, which is transmitted by the cixiidae Hyalesthes obsoletus. A reliable diagnosis has been developed, and permitted to secure the specification of phytosanitary certified material, according to the specifications “healthy plants”. A large epidemiological survey identified 16 genotypes of the stolbur phytoplasm. The study demonstrated that the epidemics mainly propagated from lavander to lavander. Finally, the study of the mechanism of tolerance showed that lavander and lavandin susceptibility was not correlated with the relation plant - phytoplasm, but more likely with the interaction of the insect with the plant. The insect is present in all cultivated plots, but in very variable densities. AFLP genotyping has also shown that genetic diversity of wild lavanders was quite restricted, and that genetic selection reduced it only a little.; La lavande et le lavandin sont des cultures emblématiques de Haute Provence, victimes d’un dépérissement depuis quelques décennies. L’agent étiologique de ce dépérissement est le phytoplasme du stolbur, et le principal vecteur de ce phytoplasme est la cicadelle Hyalesthes obsoletus. Une technique de diagnostic sensible et fiable a été mise au point, et a permis de sécuriser la certification du matériel végétal produit selon le cahier des charges plants sains. Une étude épidémiologique de grande envergure a montré que les parcelles étaient infectées par 16 génotypes différents du phytoplasme du stolbur, et que la maladie se propageait surtout de lavande(in) à lavande(in), et de lavanderaies à lavanderaies. Enfin, l’étude du phénomène de tolérance a montré que la sensibilité au dépérissement ne serait pas liée à la relation plante-phytoplasme, mais plus vraisemblablement à l’interaction de la cicadelle avec les plantes. Cette cicadelle est présente dans toutes les parcelles cultivées, mais à des effectifs très variables. Un génotypage AFLP a également montré que la diversité génétique des lavandes sauvages était assez restreinte, et que la sélection génétique effectuée par la filière l’avait très légèrement réduite.
- Published
- 2012
9. Amélioration des stratégies de lutte contre le dépérissement de la lavande et du lavandin
- Author
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Foissac, Xavier, Verdin, Eric, Nicolè, F., Bouverat-Bernier , J.P., Jagoueix-Eveillard , Sandrine, Semetey, O., Gaudin, J., Fontaine, M., Danet, Jean-Luc, Moja, S., Conord , C., Jullien , F., Legendre , L., Gallois, P., and Chaisse, Eric
- Subjects
cicadelle ,phytoplasme ,lavande ,lavandin ,dépérissement ,phytoplasme du stolbur ,épidémiologie ,diagnostic ,tolérance ,Hyalesthes obsoletus ,étiologie virale - Abstract
La lavande et le lavandin sont des cultures emblématiques de Haute Provence, victimes d’un dépérissement depuis quelques décennies. L’agent étiologique de ce dépérissement est le phytoplasme du stolbur, et le principal vecteur de ce phytoplasme est la cicadelle Hyalesthes obsoletus. Une technique de diagnostic sensible et fiable a été mise au point, et a permis de sécuriser la certification du matériel végétal produit selon le cahier des charges plants sains. Une étude épidémiologique de grande envergure a montré que les parcelles étaient infectées par 16 génotypes différents du phytoplasme du stolbur, et que la maladie se propageait surtout de lavande(in) à lavande(in), et de lavanderaies à lavanderaies. Enfin, l’étude du phénomène de tolérance a montré que la sensibilité au dépérissement ne serait pas liée à la relation plante-phytoplasme, mais plus vraisemblablement à l’interaction de la cicadelle avec les plantes. Cette cicadelle est présente dans toutes les parcelles cultivées, mais à des effectifs très variables. Un génotypage AFLP a également montré que la diversité génétique des lavandes sauvages était assez restreinte, et que la sélection génétique effectuée par la filière l’avait très légèrement réduite., Lavander and lavandin are symbolic crops in south-eastern France, in the region of Haute-Provence, but they have been affected by yellow decline for decades. The etiological agent which is involved in this decline is the stolbur phytoplasm, which is transmitted by the cixiidae Hyalesthes obsoletus. A reliable diagnosis has been developed, and permitted to secure the specification of phytosanitary certified material, according to the specifications “healthy plants”. A large epidemiological survey identified 16 genotypes of the stolbur phytoplasm. The study demonstrated that the epidemics mainly propagated from lavander to lavander. Finally, the study of the mechanism of tolerance showed that lavander and lavandin susceptibility was not correlated with the relation plant - phytoplasm, but more likely with the interaction of the insect with the plant. The insect is present in all cultivated plots, but in very variable densities. AFLP genotyping has also shown that genetic diversity of wild lavanders was quite restricted, and that genetic selection reduced it only a little.
- Published
- 2012
10. Genome size and plastid trnK-matK markers give new insights into the evolutionary history of the genus Lavandula L.
- Author
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Moja, S., primary, Guitton, Y., additional, Nicolè, F., additional, Legendre, L., additional, Pasquier, B., additional, Upson, T., additional, and Jullien, F., additional
- Published
- 2015
- Full Text
- View/download PDF
11. Genome size and plastid trnK-matK markers give new insights into the evolutionary history of the genus Lavandula L.
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Moja, S., Guitton, Y., Nicolè, F., Legendre, L., Pasquier, B., Upson, T., and Jullien, F.
- Subjects
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GENETIC markers in plants , *PLANT evolution , *PLANT genomes , *PLASTIDS , *PHYTOGEOGRAPHY , *PLANT phylogeny - Abstract
The genusLavandulaL. consists of 39 species distributed from the North Atlantic Islands, across the Mediterranean Basin to India. We analysed 36 taxa of the genusLavandularepresenting two of the three subgenera and six of the eight sections according to the most recent classification (Upson & Andrews 2004). We achieved a phylogenetic reconstruction from partial sequences from plastid trnK and matK genes; the genome size was estimated by flow cytometer measurements. The primary aim was to track phylogenetic patterns through the maternal inherited marker at the sectional level and identify possible genome duplications. The cpDNA tree shows the phylogenetic relationships between subgenus, sections and also elucidates for the first time the relationships between the endemic species of Macaronesia, Morocco and Arabia. The ancestral split between the two subgenera could be explained by dispersal followed by an early vicariance event. TheC-value shows genome up-sizing within several phylogenetic clades and geographical areas. An ancestral genome-up sizing is characterized at the node of sectionDentataeandLavandula. The cpDNA tree suggests that the taxaL. angustifoliasubsp.pyrenaica(DC.) Guinea andL. stoechassubsp.luiseiriare best treated as a distinct species. [ABSTRACT FROM PUBLISHER]
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- 2016
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12. Total Petroleum Hydrocarbons and Trace Metals in Street Dusts from Tshwane Metropolitan Area, South Africa
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OKONKWO, J. O., primary, AWOFOLU, O. R., additional, MOJA, S. J., additional, FORBES, P. C. B., additional, and SENWO, Z. N., additional
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- 2006
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13. Heterogeneity of Three Molecular Data Partition Phylogenies of Mints Related to M. × piperita (Mentha; Lamiaceae)
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Gobert, V., primary, Moja, S., additional, Taberlet, P., additional, and Wink, M., additional
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- 2006
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14. High efficiency transformation of peppermint (Mentha × piperita L.) with Agrobacterium tumefaciens
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Diemer, F., primary, Jullien, F., additional, Faure, O., additional, Moja, S., additional, Colson, M., additional, Matthys-Rochon, E., additional, and Caissard, J.C., additional
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- 1998
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15. Altered monoterpene composition in transgenic mint following the introduction of 4S-limonene synthase
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Diemer, F., Caissard, J. C., Moja, S., Chalchat, J. C., and Jullien, F.
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- 2001
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16. High efficiency transformation of peppermint (Mentha x piperita L.) with Agrobacterium tumefaciens
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Diemer, F., Jullien, F., Faure, O., Moja, S., Colson, M., Matthys-Rochon, E., and Caissard, J. C.
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- 1998
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17. Elemental composition and potential health risk of vegetable cultivated in residential area situated close to abandoned gold mine dump: Characteristics of soil quality on the vegetables.
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Mngadi S, Nomngongo PN, and Moja S
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- South Africa, Humans, Risk Assessment, Food Contamination analysis, Environmental Monitoring, Metals, Heavy analysis, Soil Pollutants analysis, Mining, Vegetables chemistry, Vegetables growth & development, Gold, Soil chemistry
- Abstract
The presence of toxic metals in residential areas near abandoned gold mine tailings is a major environmental issue. This study mainly aimed to investigate the elemental distribution of both toxic and essential elements in soils and leafy vegetables ( Brassica oleracea ) collected from eight different sites around the Davidsonville residential area, located closer to the abandoned Princess gold mine dump, Johannesburg, South Africa. The nutritional value of vegetables in the human diet was determined to assess their value to their health. The vegetables contained metals in the following descending order: Ca > Mg > Ca > Sb > Pb > Fe > Mo > Cr > Se > As > V > Ni > Co > Cd. The bioaccumulation factor (BAF) revealed that vegetables tend to accumulate most metals even (toxic) during the transfer and translocation process. Based on the recommended daily allowance (%RDA) the vegetables showed to contribute 152%, 84% and 75% toward RDA for Se, V and Ca, respectively for most adults and these play a role in human metabolic activities. The vegetables were found to be a good source of essential elements (Ca, Mg, Ni, Na, Fe) but with some traces of toxic metals such as Pb, As and Sb. Based on the health risk assessment, the vegetable posed an adverse health hazard for human consumption due to metals with high HRI >1.
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- 2024
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18. Duplication and Specialization of NUDX1 in Rosaceae Led to Geraniol Production in Rose Petals.
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Conart C, Saclier N, Foucher F, Goubert C, Rius-Bony A, Paramita SN, Moja S, Thouroude T, Douady C, Sun P, Nairaud B, Saint-Marcoux D, Bahut M, Jeauffre J, Hibrand Saint-Oyant L, Schuurink RC, Magnard JL, Boachon B, Dudareva N, Baudino S, and Caissard JC
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- Acyclic Monoterpenes, Domestication, Rosa genetics, Rosa metabolism, Rosaceae
- Abstract
Nudix hydrolases are conserved enzymes ubiquitously present in all kingdoms of life. Recent research revealed that several Nudix hydrolases are involved in terpenoid metabolism in plants. In modern roses, RhNUDX1 is responsible for formation of geraniol, a major compound of rose scent. Nevertheless, this compound is produced by monoterpene synthases in many geraniol-producing plants. As a consequence, this raised the question about the origin of RhNUDX1 function and the NUDX1 gene evolution in Rosaceae, in wild roses or/and during the domestication process. Here, we showed that three distinct clades of NUDX1 emerged in the Rosoidae subfamily (Nudx1-1 to Nudx1-3 clades), and two subclades evolved in the Rosa genus (Nudx1-1a and Nudx1-1b subclades). We also showed that the Nudx1-1b subclade was more ancient than the Nudx1-1a subclade, and that the NUDX1-1a gene emerged by a trans-duplication of the more ancient NUDX1-1b gene. After the transposition, NUDX1-1a was cis-duplicated, leading to a gene dosage effect on the production of geraniol in different species. Furthermore, the NUDX1-1a appearance was accompanied by the evolution of its promoter, most likely from a Copia retrotransposon origin, leading to its petal-specific expression. Thus, our data strongly suggest that the unique function of NUDX1-1a in geraniol formation was evolved naturally in the genus Rosa before domestication., (© The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.)
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- 2022
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19. Optimized Antibacterial Effects in a Designed Mixture of Essential Oils of Myrtus communis , Artemisia herba-alba and Thymus serpyllum for Wide Range of Applications.
- Author
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Ouedrhiri W, Mechchate H, Moja S, Baudino S, Saleh A, Al Kamaly OM, Grafov A, and Greche H
- Abstract
Nowadays, the combination of molecules influences their biological effects, and interesting outcomes can be obtained from different component interactions. Using a mixture design method, this research seeks to simulate the efficacy of essential oil combinations against various bacteria and forecast the ideal combination. The chemical compositions of Myrtus communis , Artemisia herba-alba and Thymus serpyllum essential oils were analyzed using CG/MS. Then, the combined antibacterial effects were evaluated by testing mixture design formulations using the microdilution bioassay. The main compounds detected for M. communis essential oil were myrtenyl acetate (33.67%), linalool (19.77%) and 1,8-cineole (10.65%). A. herba-alba had piperitone as a chemotype, representing 85%. By contrast, the T. serpyllum oil contained thymol (17.29%), γ-terpinene (18.31%) and p-cymene (36.15%). The antibacterial effect of the essential oils studied, and the optimum mixtures obtained were target strain-dependent. T. serpyllum alone ensured the optimal inhibition against S. aureus and E. coli , while a ternary mixture consisting of 17.1%, 39.6% and 43.1% of M. communis , A. herba-alba and T. serpyllum respectively, was associated with optimal inhibitory activity against B. subtilis . The outcome of this research supports the idea of the boosting effect of essential oil combinations toward better activities, giving better understanding of the usefulness of mixture designs for food, cosmetics, and pharmaceutical applications.
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- 2022
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20. Boosted Antioxidant Effect Using a Combinatory Approach with Essential Oils from Origanum compactum , Origanum majorana , Thymus serpyllum, Mentha spicata, Myrtus communis , and Artemisia herba-alba : Mixture Design Optimization.
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Ouedrhiri W, Mechchate H, Moja S, Mothana RA, Noman OM, Grafov A, and Greche H
- Abstract
Several studies have demonstrated the possible synergistic effect as an effective strategy to boost the bioactivity of essential oils. Using this framework, this study was conducted to effectively establish the ideal combination of six essential oils from different plants ( Origanum compactum , Origanum majorana , Thymus serpyllum , Mentha spicata , Myrtus communis , and Artemisia herba-alba ) that would express the best antioxidant activity. Each mixture was optimized using a mixture design approach to generate the most effective blend. The 2,2-diphenyl-1-picrylhydrazyl radical scavenging method was used as a reference method to assess the antioxidant activity. Each essential oil's composition was identified using the GC/MS method. The single essential oil activities demonstrated variable antioxidant effects, and following the mixture design approach, the optimal antioxidant blend was revealed, as two mixtures demonstrated the best antiradical activity with 79.46% obtained with the mixture of O. majorana (28%) and M. spicata (71%) and 78.8% obtained with the mixture O. compactum (64%), O. majorana (13%), and T. serpyllum (21%). This study proposes a practical way to elaborate mixtures in the search for a boosting effect that can be oriented for the food or pharmaceutical industry.
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- 2021
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21. Erratum: Despinasse et al. Structure of the Chemical and Genetic Diversity of the True Lavender over Its Natural Range. Plants 2020, 9 , 1640.
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Despinasse Y, Moja S, Soler C, Jullien F, Pasquier B, Bessière JM, Baudino S, and Nicolè F
- Abstract
As requested by the Editorial Office, the authors remove the scientific consortium "Camille Nous" from the author list and the Author Contributions section in the published paper [...].
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- 2021
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22. Structure of the Chemical and Genetic Diversity of the True Lavender over Its Natural Range.
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Despinasse Y, Moja S, Soler C, Jullien F, Pasquier B, Bessière JM, Noûs C, Baudino S, and Nicolè F
- Abstract
The true lavender Lavandula angustifolia Miller is a Mediterranean aromatic shrub widely cultivated for its high quality essential oil used in perfumery and phytotherapy. Despite its economic importance, the intra-specific diversity among wild, non-cultivated plants remains poorly understood. We analyzed the structure of the chemical and genetic diversity of plants from 14 sites sampled over the entire native range of the true lavender. Volatile organic compounds of inflorescences were analyzed using gas chromatography coupled to mass spectrometry. Genotyping was performed with fingerprinting genetic markers. To limit the influence of environmental variability on chemical composition, plants were grown in the same conditions in a common garden. Without prior knowledge, discriminant analysis of principal component identified unambiguously four distinct chemotypes among three genetic populations. Co-inertia analysis and supervised analysis which integrated multiple datasets indicated a strong congruency between chemical and genetic patterns. Two distinct genetic units were located at the edge of the distribution area in the south of Italy and in the northeast of Spain, and were associated with two distinct chemotypes. Our results confirmed the existence of three genetically distinct entities, suggesting speciation. All French populations and the Italian Piedmontese population were genetically homogeneous but separated in two distinct chemotypes. The dominant chemotype was present in the center of the native range in southeastern France and was at the origin of the current most cultivated French varieties. Its main compounds were linalyl acetate, linalool, and caryophyllene oxide. The second French chemotype was found in south of Massif Central and presented high abundance of valuable linalyl and lavandulyl acetates. Linalool, eucalyptol, β-caryophyllene, borneol, camphor, and cis-sabinene-hydrate were significantly associated with southern latitudes and their role would be worth exploring., Competing Interests: The authors declare no conflict of interest.
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- 2020
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23. Antioxidant and antibacterial activities of Pelargonium asperum and Ormenis mixta essential oils and their synergistic antibacterial effect.
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Ouedrhiri W, Balouiri M, Bouhdid S, Harki EH, Moja S, and Greche H
- Subjects
- Anti-Bacterial Agents chemistry, Antioxidants chemistry, Gas Chromatography-Mass Spectrometry, Microbial Sensitivity Tests, Oils, Volatile chemistry, Phytochemicals analysis, Phytochemicals pharmacology, Staphylococcus aureus drug effects, Staphylococcus aureus growth & development, Anti-Bacterial Agents pharmacology, Antioxidants pharmacology, Asteraceae, Oils, Volatile pharmacology, Pelargonium
- Abstract
In this work, the chemical composition, the antioxidant, and the antibacterial activities of two Moroccan essential oils less studied, extracted from Pelargonium asperum and Ormenis mixta, were investigated. According to the gas chromatography coupled to mass spectrometry analysis, citronellol (25.07%), citronellyl ester (10.52%), geraniol (10.46%), and buthyl anthranilate (5.93%) were found to be the major components of P. asperum, while O. mixta was mainly composed of D-germacrene (11.46%), 1,8-cineole (10.28%), and cis-methyl isoeugenol (9.04%). Moreover, O. mixta essential oil exhibited an important antioxidant activity being significantly higher than that exhibited by P. asperum oil (P < 0.001). As regards the antimicrobial activity of both essential oils, the zones of growth inhibition and the minimum inhibitory concentration values showed that P. asperum essential oil was more active than that of O. mixta. Thereafter, the impact of the binary combination of essential oils on their antimicrobial effect was investigated against Staphylococcus aureus using the fractional inhibitory concentration index calculation. The results showed a promising synergistic antibacterial interaction between essential oils studied.
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- 2018
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24. The Rosa genome provides new insights into the domestication of modern roses.
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Raymond O, Gouzy J, Just J, Badouin H, Verdenaud M, Lemainque A, Vergne P, Moja S, Choisne N, Pont C, Carrère S, Caissard JC, Couloux A, Cottret L, Aury JM, Szécsi J, Latrasse D, Madoui MA, François L, Fu X, Yang SH, Dubois A, Piola F, Larrieu A, Perez M, Labadie K, Perrier L, Govetto B, Labrousse Y, Villand P, Bardoux C, Boltz V, Lopez-Roques C, Heitzler P, Vernoux T, Vandenbussche M, Quesneville H, Boualem A, Bendahmane A, Liu C, Le Bris M, Salse J, Baudino S, Benhamed M, Wincker P, and Bendahmane M
- Subjects
- Domestication, Flowers genetics, Gene Expression Regulation, Plant, Genes, Plant, Genetic Variation, Genotype, Plant Proteins genetics, Sequence Analysis, DNA methods, Whole Genome Sequencing methods, Genome, Plant, Rosa genetics
- Abstract
Roses have high cultural and economic importance as ornamental plants and in the perfume industry. We report the rose whole-genome sequencing and assembly and resequencing of major genotypes that contributed to rose domestication. We generated a homozygous genotype from a heterozygous diploid modern rose progenitor, Rosa chinensis 'Old Blush'. Using single-molecule real-time sequencing and a meta-assembly approach, we obtained one of the most comprehensive plant genomes to date. Diversity analyses highlighted the mosaic origin of 'La France', one of the first hybrids combining the growth vigor of European species and the recurrent blooming of Chinese species. Genomic segments of Chinese ancestry identified new candidate genes for recurrent blooming. Reconstructing regulatory and secondary metabolism pathways allowed us to propose a model of interconnected regulation of scent and flower color. This genome provides a foundation for understanding the mechanisms governing rose traits and should accelerate improvement in roses, Rosaceae and ornamentals.
- Published
- 2018
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25. Bornyl-diphosphate synthase from Lavandula angustifolia: A major monoterpene synthase involved in essential oil quality.
- Author
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Despinasse Y, Fiorucci S, Antonczak S, Moja S, Bony A, Nicolè F, Baudino S, Magnard JL, and Jullien F
- Subjects
- Alkyl and Aryl Transferases genetics, Alkyl and Aryl Transferases metabolism, Amino Acid Sequence, Camphanes chemistry, Camphor chemistry, Catalytic Domain, Cloning, Molecular, Flowers enzymology, Intramolecular Lyases genetics, Models, Molecular, Phylogeny, Plant Leaves enzymology, Plant Proteins genetics, Salvia officinalis enzymology, Structure-Activity Relationship, Intramolecular Lyases metabolism, Lavandula enzymology, Oils, Volatile chemistry, Plant Oils chemistry, Plant Proteins metabolism
- Abstract
Lavender essential oils (EOs) of higher quality are produced by a few Lavandula angustifolia cultivars and mainly used in the perfume industry. Undesirable compounds such as camphor and borneol are also synthesized by lavender leading to a depreciated EO. Here, we report the cloning of bornyl diphosphate synthase of lavender (LaBPPS), an enzyme that catalyzes the production of bornyl diphosphate (BPP) and then by-products such as borneol or camphor, from an EST library. Compared to the BPPS of Salvia officinalis, the functional characterization of LaBPPS showed several differences in amino acid sequence, and the distribution of catalyzed products. Molecular modeling of the enzyme's active site suggests that the carbocation intermediates are more stable in LaBPPS than in SoBPPS leading probably to a lower efficiency of LaBPPS to convert GPP into BPP. Quantitative RT-PCR performed from leaves and flowers at different development stages of L. angustifolia samples show a clear correlation between transcript level of LaBPPS and accumulation of borneol/camphor, suggesting that LaBPPS is mainly responsible of in vivo biosynthesis of borneol/camphor in fine lavender. A phylogenetic analysis of terpene synthases (TPS) pointed out the basal position of LaBPPS in the TPSb clade, suggesting that LaBPPS could be an ancestor of others lavender TPSb. Finally, borneol could be one of the first monoterpenes to be synthesized in the Lavandula subgenus. Knowledge gained from these experiments will facilitate future studies to improve the lavender oils through metabolic engineering or plant breeding. Accession numbers: LaBPPS: KM015221., (Copyright © 2017. Published by Elsevier Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
26. Evidence for early intracellular accumulation of volatile compounds during spadix development in Arum italicum L. and preliminary data on some tropical Aroids.
- Author
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Leguet A, Gibernau M, Shintu L, Caldarelli S, Moja S, Baudino S, and Caissard JC
- Subjects
- Araceae growth & development, Arum growth & development, Chromatography, Gas, Plant Leaves chemistry, Terpenes analysis, Araceae chemistry, Arum chemistry, Pollination, Volatile Organic Compounds analysis
- Abstract
Staining and histochemistry of volatile organic compounds (VOCs) were performed at different inflorescence developmental stages on nine aroid species; one temperate, Arum italicum and eight tropical from the genera Caladium, Dieffenbachia and Philodendron. Moreover, a qualitative and quantitative analysis of VOCs constituting the scent of A. italicum, depending on the stage of development of inflorescences was also conducted. In all nine species, vesicles were observed in the conical cells of either the appendix or the stamens (thecae) and the staminodes. VOCs were localised in intracellular vesicles from the early stages of inflorescence development until their release during receptivity of gynoecium. This localisation was observed by the increase of both number and diameter of the vesicles during 1 week before receptivity. Afterwards, vesicles were fewer and smaller but rarely absent. In A. italicum, staining and gas chromatography analyses confirmed that the vesicles contained terpenes. The quantitatively most important ones were the sesquiterpenes, but monoterpenes were not negligible. Indeed, the quantities of terpenes matched the vesicles' size evolution during 1 week. Furthermore, VOCs from different biosynthetic pathways (sesquiterpenes and alkanes) were at their maximum quantity 2 days before gynoecium receptivity (sesquiterpenes and alkanes) or during receptivity (isobutylamine, monoterpenes, skatole and p-cresol). VOCs seemed to be emitted during gynoecium receptivity and/or during thermogenesis, and FADs are accumulated after thermogenesis in the spadix. These complex dynamics of the different VOCs could indicate specialisation of some VOCs and cell machinery to attract pollinators on the one hand and to repulse/protect against phytophagous organisms and pathogens after pollination on the other hand.
- Published
- 2014
- Full Text
- View/download PDF
27. Isolation and functional characterization of a τ-cadinol synthase, a new sesquiterpene synthase from Lavandula angustifolia.
- Author
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Jullien F, Moja S, Bony A, Legrand S, Petit C, Benabdelkader T, Poirot K, Fiorucci S, Guitton Y, Nicolè F, Baudino S, and Magnard JL
- Subjects
- Alkyl and Aryl Transferases genetics, Amino Acid Sequence, Lavandula genetics, Models, Molecular, Molecular Sequence Data, Mutagenesis, Phylogeny, Plant Proteins genetics, Plant Proteins metabolism, Protein Conformation, RNA, Plant genetics, RNA, Plant metabolism, Alkyl and Aryl Transferases metabolism, Gene Expression Regulation, Enzymologic physiology, Gene Expression Regulation, Plant physiology, Lavandula enzymology, Sesquiterpenes metabolism
- Abstract
In this paper we characterize three sTPSs: a germacrene D (LaGERDS), a (E)-β-caryophyllene (LaCARS) and a τ-cadinol synthase (LaCADS). τ-cadinol synthase is reported here for the first time and its activity was studied in several biological models including transiently or stably transformed tobacco species. Three dimensional structure models of LaCADS and Ocimum basilicum γ-cadinene synthase were built by homology modeling using the template structure of Gossypium arboreum δ-cadinene synthase. The depiction of their active site organization provides evidence of the global influence of the enzymes on the formation of τ-cadinol: instead of a unique amino-acid, the electrostatic properties and solvent accessibility of the whole active site in LaCADS may explain the stabilization of the cadinyl cation intermediate. Quantitative PCR performed from leaves and inflorescences showed two patterns of expression. LaGERDS and LaCARS were mainly expressed during early stages of flower development and, at these stages, transcript levels paralleled the accumulation of the corresponding terpene products (germacrene D and (E)-β-caryophyllene). By contrast, the expression level of LaCADS was constant in leaves and flowers. Phylogenetic analysis provided informative results on potential duplication process leading to sTPS diversification in lavender.
- Published
- 2014
- Full Text
- View/download PDF
28. MSeasy: unsupervised and untargeted GC-MS data processing.
- Author
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Nicolè F, Guitton Y, Courtois EA, Moja S, Legendre L, and Hossaert-McKey M
- Subjects
- Algorithms, Cluster Analysis, Data Interpretation, Statistical, Gas Chromatography-Mass Spectrometry methods, Software
- Abstract
Unlabelled: MSeasy performs unsupervised data mining on gas chromatography-mass spectrometry data. It detects putative compounds within complex metabolic mixtures through the clustering of mass spectra. Retention times or retention indices are used after clustering, together with other validation criteria, for quality control of putative compounds. The package generates a fingerprinting or profiling matrix compatible with NIST mass spectral search program and ARISTO webtool (Automatic Reduction of Ion Spectra To Ontology) for molecule identification. Most commonly used file formats, NetCDF, mzXML and ASCII, are acceptable. A graphical and user-friendly interface, MSeasyTkGUI, is available for R novices., Availability: MSeasy and MSeasytkGUI are implemented as R packages available at http://cran.r-project.org/web/packages/MSeasy/index.html and http://cran.r-project.org/web/packages/MSeasyTkGUI/index.html.
- Published
- 2012
- Full Text
- View/download PDF
29. Extracellular localization of the diterpene sclareol in clary sage (Salvia sclarea L., Lamiaceae).
- Author
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Caissard JC, Olivier T, Delbecque C, Palle S, Garry PP, Audran A, Valot N, Moja S, Nicolé F, Magnard JL, Legrand S, Baudino S, and Jullien F
- Subjects
- Chemistry, Organic methods, Crystallization, Diterpenes chemistry, Gas Chromatography-Mass Spectrometry methods, Gene Expression Regulation, Plant, Ions, Mass Spectrometry methods, Microscopy, Electron, Scanning methods, Oils, Plant Extracts chemistry, Plant Proteins metabolism, Temperature, Terpenes, Salvia metabolism
- Abstract
Sclareol is a high-value natural product obtained by solid/liquid extraction of clary sage (Salvia sclarea L.) inflorescences. Because processes of excretion and accumulation of this labdane diterpene are unknown, the aim of this work was to gain knowledge on its sites of accumulation in planta. Samples were collected in natura or during different steps of the industrial process of extraction (steam distillation and solid/liquid extraction). Samples were then analysed with a combination of complementary analytical techniques (gas chromatography coupled to a mass spectrometer, polarized light microscopy, environmental scanning electron microscopy, two-photon fluorescence microscopy, second harmonic generation microscopy). According to the literature, it is hypothesized that sclareol is localized in oil pockets of secretory trichomes. This study demonstrates that this is not the case and that sclareol accumulates in a crystalline epicuticular form, mostly on calyces.
- Published
- 2012
- Full Text
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30. Lavender inflorescence: a model to study regulation of terpenes synthesis.
- Author
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Guitton Y, Nicolè F, Moja S, Benabdelkader T, Valot N, Legrand S, Jullien F, and Legendre L
- Abstract
We analysed VOC composition of complete inflorescences and single flowers of lavender during the flowering period. Our analyses, focused on the 20 most abundant terpenes, showed that three groups of components could be separated according to their patterns of variation during inflorescence ontogeny. These three groups were associated with three developmental stages: flower in bud, flower in bloom and faded flower. The expression of two terpene synthases (TPS) was followed using qPCR during inflorescence ontogeny. A comparison of these chemical and molecular analyses suggested that VOC production in lavender spike is mainly regulated at the transcriptional level. These results highlighted that lavender could be a model plant for future investigations on terpene biosynthesis and regulation, and could be used to explore the functions of terpene metabolites.
- Published
- 2010
- Full Text
- View/download PDF
31. Differential accumulation of volatile terpene and terpene synthase mRNAs during lavender (Lavandula angustifolia and L. x intermedia) inflorescence development.
- Author
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Guitton Y, Nicolè F, Moja S, Valot N, Legrand S, Jullien F, and Legendre L
- Subjects
- Gas Chromatography-Mass Spectrometry, Gene Expression Profiling, Gene Expression Regulation, Plant, Genes, Plant, Hydro-Lyases genetics, Inflorescence enzymology, Inflorescence genetics, Inflorescence growth & development, Intramolecular Lyases genetics, Lavandula enzymology, Microscopy, Electron, Scanning, Oils, Volatile analysis, Plant Leaves chemistry, Plant Leaves enzymology, Plant Leaves genetics, Plant Leaves growth & development, Plant Oils analysis, Plant Proteins genetics, RNA, Messenger metabolism, RNA, Plant metabolism, Volatile Organic Compounds analysis, Hydro-Lyases metabolism, Inflorescence chemistry, Intramolecular Lyases metabolism, Lavandula genetics, Plant Proteins metabolism, Terpenes metabolism
- Abstract
Despite the commercial importance of Lavandula angustifolia Mill. and L. x intermedia Emeric ex Loisel floral essential oils (EOs), no information is currently available on potential changes in individual volatile organic compound (VOC) content during inflorescence development. Calyces were found to be the main sites of VOC accumulation. The 20 most abundant VOCs could be separated into three sub-groups according to their patterns of change in concentration The three groups of VOCs sequentially dominated the global scent bouquet of inflorescences, the transition between the first and second groups occurring around the opening of the first flower of the inflorescence and the one between the second and third groups at the start of seed set. Changes in calyx VOC accumulation were linked to the developmental stage of individual flowers. Leaves accumulated a smaller number of VOCs which were a subset of those seen in preflowering inflorescences. Their nature and content remained constant during the growing season. Quantitative real time polymerase chain reaction assessments of the expression of two terpene synthase (TPS) genes, LaLIMS and LaLINS, revealed similar trends between their patterns of expression and those of their VOC products. Molecular and chemical analyses suggest that changes in TPS expression occur during lavender inflorescence development and lead to changes in EO composition. Both molecular data and terpene analysis support the findings that changes in biosynthesis of terpene occurred during inflorescence development.
- Published
- 2010
- Full Text
- View/download PDF
32. One-step identification of conserved miRNAs, their targets, potential transcription factors and effector genes of complete secondary metabolism pathways after 454 pyrosequencing of calyx cDNAs from the Labiate Salvia sclarea L.
- Author
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Legrand S, Valot N, Nicolé F, Moja S, Baudino S, Jullien F, Magnard JL, Caissard JC, and Legendre L
- Subjects
- Base Sequence, DNA, Complementary genetics, DNA, Plant genetics, Expressed Sequence Tags, Gene Library, Salvia metabolism, Conserved Sequence, Flowers genetics, Metabolic Networks and Pathways genetics, MicroRNAs genetics, Salvia genetics, Sequence Analysis, DNA methods, Transcription Factors genetics
- Abstract
The outermost floral whorl, composed of sepals, is generally thought to function in the protection of reproductive tissues. In the plant family Lamiaceae, sepals are fused into a tube that is densely covered by hairs for mechanical defence and contains secondary metabolites for chemical defence against insects and abiotic stresses. Despite the importance of this tissue in plant fitness, virtually no study has addressed the basic aspects of sepal development and functioning. Because of its large size and its impressive metabolic activity (both in terms of quantity and diversity of secondary metabolites), we have used clary sage calyx as a model system to generate the first high throughput sequencing of the transcriptome of an angiosperm calyx. We applied massive parallel 454 pyrosequencing technology to a normalized cDNA extract and unveiled potential candidate genes for all steps of secondary metabolite pathways (phenylpropanoids and terpenoids). It also proved efficient in predicting the expression of large numbers of transcription factors and, with the use of bioinformatics tools, it predicted in the same sequencing run the presence of a novel class of gene transcription regulatory elements, miRNAs, without the need to generate a separate miRNA library. In our clary sage EST library, 18 conserved miRNAs were predicted. Among them, 15 were present in most studied plant species while the others were only shared with limited or discrete plant lineages. A separate data mining of the same clary sage EST library suggested the presence of 19 potential target genes to the 18 predicted conserved miRNAs. These coded for only 6 transcription factors or F-box proteins, 11 metabolism or abiotic stress response related proteins and 2 products with no known predicted function. All in all, this study provides novel genomic information on an angiosperm calyx and an experimental framework to predict in a single step metabolic pathway enzymes and regulator genes including miRNAs.
- Published
- 2010
- Full Text
- View/download PDF
33. Heterogeneity of three molecular data partition phylogenies of mints related to M. x piperita (Mentha; Lamiaceae).
- Author
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Gobert V, Moja S, Taberlet P, and Wink M
- Subjects
- Haplotypes, Mentha classification, Mentha piperita genetics, Polymorphism, Genetic, Pseudogenes, DNA, Plant chemistry, Mentha genetics, Phylogeny
- Abstract
Phylogenetic reconstructions with molecular tools are now widely used, thanks to advances in PCR and sequencing technologies. The choice of the molecular target still remains a problem because too few comparative data are available. This is particularly true for hybrid taxa, where differential introgression of genome parts leads to incongruity between data sets. We have studied the potential of three data partitions to reconstruct the phylogeny of mints related to M. x piperita. These included nuclear DNA (ITS), chloroplast DNA (non-coding regions trnL intron, intergenic spacers trnL-trnF, and psbA-trnH), and AFLP and ISSR, markers. The taxonomic sampling was composed of hybrids, diploid and polyploid genomes. Since the genealogy of cultivated mint hybrids is known, they represent a model group to compare the usefulness of various molecular markers for phylogeny inference. Incongruities between ITS, chloroplast DNA, and AFLP-ISSR phylogenetic trees were recorded, although DNA fingerprinting data were congruent with morphological classification. Evidence of chloroplast capture events was obtained for M. x piperita. Direct sequencing of ITS led to biased results because of the existence of pseudogenes. Sequencing of cloned ITS further failed to provide evidence of the existence of the two parental copy types for M. x piperita, a sterile hybrid that has had no opportunity for concerted evolution of ITS copies. AFLP-ISSR data clustered M. x piperita with the parent that had the largest genome. This study sheds light on differential of introgression of different genome regions in mint hybrids.
- Published
- 2006
- Full Text
- View/download PDF
34. Solid support quantitation of c-myc PCR products using a cleavable reporter.
- Author
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Rhoer-Moja S, Bazin H, Sauvaigo S, Chypre C, and Vindimian M
- Subjects
- Base Sequence, Gene Amplification, Humans, Molecular Sequence Data, DNA Primers chemistry, Genes, myc, Polymerase Chain Reaction methods
- Published
- 1994
- Full Text
- View/download PDF
35. Detection of quantitative polymerase chain reaction products by hybridization on magnetic support with 125I-radiolabeled probes: quantification of c-myc copy numbers.
- Author
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Rhoer-Moja S, Cohen-Haguenauer O, Jouve C, Healy JC, and Vindimian M
- Subjects
- Bacterial Proteins, Base Sequence, Biotin, Humans, Molecular Sequence Data, Nucleic Acid Hybridization, Reference Standards, Sensitivity and Specificity, Streptavidin, Tumor Cells, Cultured, DNA Probes, Genes, myc genetics, Iodine Radioisotopes, Magnetics, Neoplasms genetics, Polymerase Chain Reaction methods
- Abstract
We present a technique for determining c-myc copy numbers that can be used as a prognosis index for some cancers. The method is based on the use of both competitive polymerase chain reaction and hybridization of amplified products. Coamplification was performed directly on cells with a synthetic oligonucleotide used as internal standard. It recognized the same primer set as the target. Coamplified products were captured on streptavidin magnetic beads as solid support using a 5' biotinylated primer. DNA immobilized on this support was denatured with alkali. Each coamplified product (target and reference gene) was further hybridized to two distinct specific oligonucleotide probes. Gene amplification levels were determined using a standard curve obtained by serial dilutions of peripheral blood lymphocytes run along with the experimental samples. This approach provides a rapid (less than 2 days) and reproducible method for evaluating c-myc gene copy number and may be used to quantify any gene. Moreover, its format allows for automation.
- Published
- 1993
- Full Text
- View/download PDF
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