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Bowhead whales generally migrate into high Arctic waters in the summer months and move to lower latitudes in the wintertime. During the 1800s and early 1900s commercial whaling greatly reduced the numbers of bowhead whales in waters adjacent to the North Atlantic Ocean. In recent decades their numbers have been increasing. Thirteen sightings of a bowhead whale were recorded in four areas of the Gulf of Maine in 2012, 2014, and 2017 between latitudes 44°43’N and 41°36’N, far south of the normal range (54°N) for this species. Photographs obtained during these sightings were compared by experienced photo analysts and, based on scarring patterns, the sightings were confirmed to be of the same individual. The bowhead whale was observed alone, in addition to interacting in a social group and engaged in coordinated feeding with other mysticetes at times. The feeding and social behaviour of the bowhead whale was typical for the species but well south of its normal Arctic waters range and in the absence of conspecifics

North Atlantic right whalesEubalaena glacialisare most commonly observed along the eastern seaboard of North America; however, their distribution and occupancy patterns have become less predictable in the last decade. This study explored the individual right whales captured photographically from both dedicated and opportunistic sources from 2015 to 2019 in the Gulf of St. Lawrence (GSL), an area previously understudied for right whale presence. A total of 187 individuals, including reproductive females, were identified from all sources over this period. In years when more substantial survey effort occurred (2017-2019), similar numbers of individuals were sighted (mean = 133, SD = 1.5), and dedicated mark-recapture aerial surveys were highly effective at capturing almost all of the whales estimated in the region (2019:N= 137, 95% CI = 135-147). A high rate of inter-annual return was observed between all 5 study years, with 95% of the animals seen in 2019 sighted previously. Capture rates indicated potential residencies as long as 5 mo, and observed behaviors included feeding and socializing. Individuals were observed in the northern and southern GSL, regions divided by a major shipping corridor. Analyses suggest that individuals mostly moved less than 9.1 km d-1, although rates of up to 79.8 km d-1were also calculated. The GSL is currently an important habitat for 40% of this Critically Endangered species, which underscores how crucial protection measures are in this area.

The population of western North Atlantic right whales (Eubalaena glacialis) is distributed primarily between Florida, USA and NovaScotia, Canada, aggregating seasonally in five geographically distinct, high-use areas. To test the effectiveness of monitoring alldemographic classes (juvenile and adult males and females) of the population in these five habitat areas, an evaluation was carried out ofthe identification records of catalogued right whales collected between 1980 and 1992, for which the age, sex and reproductive status (foradult females) were known. The mean annual identification frequency of adult females was significantly lower than that of adult males,juvenile females and juvenile males. Among adult females, reproductively active females were seen significantly more often than expectedwhen lactating (with a calf) than during their pregnancy or resting years. These data suggest that, while research efforts in the five high-usehabitat areas have had relatively equal success at monitoring juvenile males and females and adult males, many adult females are segregatedat times from the rest of the population. Lower variability in annual identification frequencies of adult females indicates that they may bemore site specific in their distribution than males, particularly during the years when they are pregnant or resting from a previous pregnancy.Re-running these analyses using sighting records updated through 2000 will help determine if the trends continue to be documentedregardless of changes in survey effort and patterns of habitat use of some animals.

Species' response to rapid climate change can be measured through shifts in timing of recurring biological events, known as phenology. The Gulf of Maine is one of the most rapidly warming regions of the ocean, and thus an ideal system to study phenological and biological responses to climate change. A better understanding of climate-induced changes in phenology is needed to effectively and adaptively manage human-wildlife conflicts. Using data from a 20+ year marine mammal observation program, we tested the hypothesis that the phenology of large whale habitat use in Cape Cod Bay has changed and is related to regional-scale shifts in the thermal onset of spring. We used a multi-season occupancy model to measure phenological shifts and evaluate trends in the date of peak habitat use for North Atlantic right (Eubalaena glacialis), humpback (Megaptera novaeangliae), and fin (Balaenoptera physalus) whales. The date of peak habitat use shifted by +18.1 days (0.90 days/year) for right whales and +19.1 days (0.96 days/year) for humpback whales. We then evaluated interannual variability in peak habitat use relative to thermal spring transition dates (STD), and hypothesized that right whales, as planktivorous specialist feeders, would exhibit a stronger response to thermal phenology than fin and humpback whales, which are more generalist piscivorous feeders. There was a significant negative effect of western region STD on right whale habitat use, and a significant positive effect of eastern region STD on fin whale habitat use indicating differential responses to spatial seasonal conditions. Protections for threatened and endangered whales have been designed to align with expected phenology of habitat use. Our results show that whales are becoming mismatched with static seasonal management measures through shifts in their timing of habitat use, and they suggest that effective management strategies may need to alter protections as species adapt to climate change.

North Atlantic right whales Eubalaena glacialis (hereafter right whales) have recently shifted their distribution away from some protected feeding habitats, which suggests large-scale changes in food supply have occurred. Quantifying the nature and extent of the apparent shift is key to exploring this hypothesis and planning or revising conservation strategies. This paper characterizes decadal right whale occurrence in the Bay of Fundy summer feeding habitat using data derived from 7522 h of survey effort collected over 30 yr (1987 to 2016) that yielded 11483 right whale sightings. Eight occurrence descriptors were derived to quantify temporal variation in right whale presence, encounter rates and time of arrival in the Bay. Time of arrival was modeled using linear and power function models fit to annual discovery curves. Principal component analysis showed 2 modes that explained 58 and 14% of the variation in occurrence, respectively. The first mode captured a significant decline in summertime right whale occurrence beginning in 2010. The second mode captured a decadal-scale cycle in seasonal timing of occurrence, which highlighted a sharp change from early to late occupancy at the turn of the millennium. Annual occurrence during the 2010-2016 period was anomalously low in both the Grand Manan Basin critical habitat and the Bay of Fundy Traffic Separation Scheme (shipping lanes), whereas encounters in the region NW of the critical habitat containing Owen Basin and The Wolves islands may be increasing due to a distributional shift within the Bay. We discuss the consequences of these changes for both population dynamics and future conservation of the species.

A survey questionnaire designed to determine mariner knowledge and awareness of endangered whales, existing conservation measures, and mariner receptivity to near real-time conservation technology on the bridge is herein reported. The survey, distributed by the Shipping Federation of Canada, yielded 43 responses. The majority of respondents were interested in receiving information on endangered whales and conservation measures in eastern Canada and USA Gulf of Maine regions (72% and 79%, respectively). Eighty-four percent of respondents indicated a preference for receiving whale alerts via Navigational telex (NAVTEX) and 79% listed NAVTEX as the most “not disruptive” means of receiving the alerts. A similar 72% also listed Automatic Identification Systems (AIS) as “not disruptive”, and 58% identified AIS as the preferred reception format. The results show that the commercial fleet is moderately receptive to near real-time whale alerts on the bridge. It is concluded that to better understand mariner willingness to participate in whale conservation, researchers should consider defining the response required of mariners when receiving such alerts. The results also suggest that future conservation programs should use communication formats that are most familiar to, and favoured by, mariners while being the least disruptive to bridge protocols; i.e., NAVTEX and AIS.

Ocean-going vessels present a measurable threat of lethal collision with many marine species worldwide, notably large whale species of which many are endangered. Various modifications to conventional vessel operations have been recently used to reduce the threat. Some of the modifications have been instituted by coastal states as a result of their adoption by the International Maritime Organization (IMO) — a specialized agency of the United Nations that is the recognized authority for international maritime shipping interests and their safety of navigation at sea. We describe the processes through which coastal states can approach the IMO to seek review and adoption of environmental conservation proposals involving international shipping. We also provide a description of vessel navigation modifications in specific geographic areas where IMO-adopted measures to protect large whales have been implemented — there are only 10 such cases and we describe each. We then address the methods that can and have been used to assess the effectiveness of such measures. As weighed against the goals of the modifications by estimating the ensuing reduced risk to whales, actions taken are generally regarded as being successful in reducing the risk, but to varying degrees. We conclude that the IMO can be a powerful entity in providing solutions to a range of marine environmental and conservation problems. When used in concert with related efforts such as mariner education, the IMO, and the range of navigational measures available to it, is an effective forum through which coastal states can pursue large whale conservation objectives without unduly compromising the activities of shipping interests.

The high variability in reproductive performance of North Atlantic right whales Eubal- aena glacialis compared to southern right whales Eubalaena australis may reflect differences in lipid reserves. Amplitude-mode ultrasound was used to measure the thickness of right whale integument (epidermis and blubber, herein referred to as blubber thickness) in E. glacialis in the Bay of Fundy, Canada for 5 summer seasons and in E. australis off the South African coast for 2 austral winter sea- sons. E. glacialis had significantly thinner blubber layers (mean ±1 SD = 12.23 ± 2.16 cm, n = 172) than E. australis (16.13 ± 3.88 cm, n = 117), suggesting differing levels of nutrition between the 2 species. Blubber was thickest in females measured 3 to 6 mo prior to the start of pregnancy (E. glacialis), thin- ner during lactation (E. glacialis, E. australis) and then thicker with time after weaning (E. glacialis). These results suggest that lipids in blubber are used as energetic support for reproduction in female right whales. Blubber thickness increased in calves during suckling (E. glacialis, E. australis) but sub - sequently decreased after weaning (E. glacialis). Juvenile and adult male E. glacialis blubber thick- nesses were compared between years of differing prey Calanus finmarchicus abundances (data from Pershing et al. 2005; ICES J Mar Sci 62:1511-1523); during a year of low prey abundance whales had significantly thinner blubber than during years of greater prey abundance. Taken together, these re- sults suggest that blubber thickness is indicative of right whale energy balance and that the marked fluctuations in North Atlantic right whale reproduction have a nutritional component.

Baleen whales have among the lowest reproduc- tive rates in the animal kingdom, coupled with high energetic demands on lactating mothers to support the rapid growth of their offspring. Because each offspring represents a large portion of a female's reproductive effort, strong selec- tion pressure should be in place to minimize the potential for misplaced parental effort. However, we describe a case in which two North Atlantic right whale (Eubalaena glacialis) offspring were switched between mothers and remained with their "adopted" mothers throughout their first year of life (until they were weaned). The most reason- able explanation is that this swap was an accident caused by the females calving in close spatial and temporal proximity. The calves likely associated with the wrong mothers before any mother-off- spring recognition system had developed, and an association then formed between these non-bio- logical mother-offspring pairs. These data raise intriguing questions regarding how often this may occur in other wildlife populations, what mecha- nisms are used for mother-offspring recognition in whales, and how long it takes for this recognition to develop.

North Atlantic right whales, Eubalaena glacialis, remain endangered, primarily due to excessive anthropogenic mortality. Current management protocols in US waters are triggered by identifying the presence of at least one right whale in a management area. We assessed whether acoustic detection of right whale contact calls can work as an alternative to visual aerial surveys for establishing their presence. Aerial survey and acoustic monitoring were conducted in Cape Cod Bay, Massachusetts, in 2001-2005 and used to evaluate and compare right whale detections. Over the 58 d with simultaneous aerial and acoustic coverage, aerial surveys saw whales on approximately two-thirds of the days during which acoustic monitoring heard whales. There was no strong relationship between numbers of whales seen during aerial surveys and numbers of contact calls detected on survey days. Results indicate acoustic monitoring is a more reliable mechanism than aerial survey for detecting right whales. Because simple detection is sufficient to trigger current management protocols, continuous, autonomous acoustic monitoring provides information of immediate management utility more reliably than aerial surveillance. Aerial surveys are still required to provide data for estimating population parameters and for visually assessing the frequency and severity of injuries from shipping and fishing and detecting injured and entangled right whales.

Many long-term studies of wildlife populations rely on individual identification based on natural markings or genetic profiling, or both. However, only rarely are these 2 independent data sets systematically compared with each other to estimate the error rates inherent in these studies. Here, >25 years of photo-identification data on the endangered North Atlantic right whale (Eubalaena glacialis) were compared with high-resolution genetic profiles, available for >75% of the individuals in the photo-identification catalog, in order to identify sources and rates of errors associated with both methods of individual identification. The resulting estimates were 0.0308 errors/identification for the photo-identification data, and 0.00121 errors/locus and 0.0327 errors/multilocus profile for the genetic data. These are among the lowest error rates yet reported, and indicate that the approaches used provide reliable means of individual identification for this species. However, despite these low error rates, the large size of the data sets results in a nonnegligible estimated number of errors, indicating that the potential for these errors needs to be incorporated into other analyses that are based on these data. A similar situation likely exists in other long-term studies where, although error rates are assumed to be low, the size of the data set results in a large number of errors that will influence subsequent analyses. Regularly conducting and reporting extensive database comparisons such as this is invaluable for maintaining the integrity of long-term data sets by identifying where sources of error are occurring and how protocols can be improved to lower error rates in the future.

Management plans to reduce human-caused deaths of North Atlantic right whales Eubalaena glacialis depend, in part, on knowing when and where right whales are likely to be found. Local environmental conditions that influence movements of feeding right whales, such as ultradense copepod patches, are unpredictable and ephemeral. We examined the utility of using the regional-scale mean copepod concentration as an indicator of the abundance of right whales in 2 critical habitats off the northeastern coast of the United States: Cape Cod Bay and Great South Channel. Right whales are usually found in Cape Cod Bay during the late winter and early spring, and in the Great South Channel during the late spring and early summer. We found a significant positive relationship between mean concentration of the copepod Calanus finmarchicus in the western Gulf of Maine and the frequency of right whale sightings in the Great South Channel. In Cape Cod Bay we found a significant positive relationship between the mean concentration of other copepods (largely Pseudocalanus spp. and Centropages spp.) and the frequency of right whale sightings. This information could be used to further our understanding of the environmental factors that drive seasonal movement and aggregation of right whales in the Gulf of Maine, and it offers a tool to resource managers and modelers who seek to predict the movements of right whales based upon the concentration of copepods.

Balancing human uses of the marine environment with the recovery of protected species requires accurate information on when and where species of interest are likely to be present. Here, we describe a system that can produce useful estimates of right whale Eubalaena glacialis presence and abundance on their feeding grounds in the Gulf of Maine. The foundation of our system is a coupled physical-biological model of the copepod Calanus finmarchicus, the preferred prey of right whales. From the modeled prey densities, we can estimate when whales will appear in the Great South Channel feeding ground. Based on our experience with the system, we consider how the relationship between right whales and copepods changes across spatial scales. The scale-dependent relationship between whales and copepods provides insight into how to improve future estimates of the distribution of right whales and other pelagic predators.

Low levels of genetic variability identified within the North Atlantic right whale (Eubalaena glacialis), when compared to the Southern right whale (E. australis) and other large whales, have been suggested to result from population reductions due to whaling. Previous genetic analysis of 218 whale bones from sixteenth century Basque whaling sites in the western North Atlantic revealed only a single right whale bone. We determined the genotypes of 27 microsatellite loci using DNA isolated from this bone. All alleles from the historic specimen occur in the extant western North Atlantic population and both the probability of identity of the specimen and the number of heterozygous loci are similar to that in the extant population. Assessments of how genetically different the historical population might have been suggest genetic characteristics have not changed substantially over four centuries of whaling.

Commercial exploitation reduced the North Atlantic right whale (Eubalaena glacialis) population from c. 12 000 in the 11th century to around 300 by the 21st century. We examine the effect of this population decline on levels of genetic variation at 16 microsatellite loci and contrast levels of variability to that in a closely related species (E. australis). Of the 13 loci developed from the E. glacialis genome, 100% were polymorphic in E. australis. In contrast, nine loci were polymorphic in E. glacialis and four were fixed. Both allelic diversity (A) and heterozygosity (H) were significantly lower in E. glacialis than E. australis (A = 3.2 ± 2.6 vs. A= 6.9 ± 3.3, P

The Massachusetts Bay (MB) and Cape Cod Bay (CCB) system is a unique coastal embayment, with CCB serving as a high-use feeding ground for endangered North Atlantic right whales Eubalaena glacialis during winter and early spring. We used a hydrodynamic model, observed Calanus finmarchicus (the preferred prey of right whales) abundance, and right whale sightings during 1997-2004 to examine the transport and retention of C. finmarchicus in MB-CCB and the implications for right whale activity. A particle-tracking program was embedded into the model to simulate the Lagrangian residual circulation, and the transport and retention of passive particles. The results indicate significant correlations in the inter-annual variability of coastal trans- port, circulation pattern, C. finmarchicus abundance, and right whale sightings in spring. In nor- mal years (with prevailing northwesterly winds), the monthly mean distributions of particles and Lagrangian residual currents show a coherent pattern with 2 particle retention areas in central CCB and central MB, respectively. The CCB retention area closely matches the area of high prob- ability of right whale occurrence and feeding activity, indicating that this is an area favorable to zooplankton aggregations. In other years (e.g. 2002), the coastal current is significantly reduced due to prevailing southwesterly winds, and the particle retention zone is shifted northward, coinci- dent with low C. finmarchicus abundance and whale sightings in CCB. The study also suggests that the North Atlantic Oscillation may influence C. finmarchicus abundance and distributions, and hence, right whale feeding activity in CCB through surface winds affecting MB-CCB circulation, and hence, zooplankton distributions.

Marine mammal social interactions are poorly understood primarily because of the difficulty of observing these behaviors in the wild. Observations of copulations in North Atlantic right whale surface active groups (SAGs) have led to the hypothesis that the function of these groups is for conception. The occurrence and composition of SAGs sighted from 1992 to 2001 were investigated on the basis of age, sex, and reproductive status of individuals. A total of 918 SAG events were documented. SAGs were observed in all habitat areas and in all months in which right whales were sighted. Group compositions revealed both potentially conceptive groups and nonconceptive groups. Of right whales whose age was known, 93% were first sighted in SAGs as juveniles, and more than half of all observed groups contained at least one juvenile whale. The group composition and timing of occurrence of SAGs do not support the hypothesis that all groups serve a purely conceptive function. Their functional role is likely much broader. Other potential functions include play, mating practice, or maintenance of social bonds.

A North Atlantic right whale (Eubalaena glacialis) genomic library was developed and screened with a (GATA)8 probe to identify tetranucleotide microsatellite loci. Sixteen characterized loci were polymorphic in North Atlantic and/or South Atlantic (Eubalaena australis) right whales, 12 being polymorphic in E. glacialis, and 15 in E. australis. Fourteen of these were combined with 21 other previously identified loci for a suite of 35 loci which can be used to increase resolution of genetic analyses of these species. Multiplex reactions were developed for genotyping samples at these loci, providing a method that is rapid, reliable and cost-effective.

Right whales carry large populations of three ‘whale lice’ ( Cyamus ovalis , Cyamus gracilis , Cyamus erraticus ) that have no other hosts. We used sequence variation in the mitochondrial COI gene to ask (i) whether cyamid population structures might reveal associations among right whale individuals and subpopulations, (ii) whether the divergences of the three nominally conspecific cyamid species on North Atlantic, North Pacific, and southern right whales ( Eubalaena glacialis, Eubalaena japonica, Eubalaena australis ) might indicate their times of separation, and (iii) whether the shapes of cyamid gene trees might contain information about changes in the population sizes of right whales. We found high levels of nucleotide diversity but almost no population structure within oceans, indicating large effective population sizes and high rates of transfer between whales and subpopulations. North Atlantic and Southern Ocean populations of all three species are reciprocally monophyletic, and North Pacific C. erraticus is well separated from North Atlantic and southern C. erraticus . Mitochondrial clock calibrations suggest that these divergences occurred around 6 million years ago (Ma), and that the Eubalaena mitochondrial clock is very slow. North Pacific C. ovalis forms a clade inside the southern C. ovalis gene tree, implying that at least one right whale has crossed the equator in the Pacific Ocean within the last 1–2 million years (Myr). Low-frequency polymorphisms are more common than expected under neutrality for populations of constant size, but there is no obvious signal of rapid, interspecifically congruent expansion of the kind that would be expected if North Atlantic or southern right whales had experienced a prolonged population bottleneck within the last 0.5 Myr.

The North Atlantic right whale, Eubalaena glacialis (Müller, 1776), is one of the world's most endangered large cetaceans. It is widely believed that Basque whalers caused the most dramatic decline of this species in the western North Atlantic during the early-16th and 17th centuries. Previous osteological analysis of 17 historic bones suggested that 50% of the Basque harvest consisted of right whales and 50% of bowhead whales, Balaena mysticetus L., 1758. This 50:50 ratio has been used to estimate pre-exploitation population size, which has subsequently formed the basis of recovery goals and plans for the North Atlantic right whale. Genetic analysis of 21 bones, 13 identified as right whales and 8 as bowhead whales through osteological examination, indicates that in fact only 1 bone was a right whale and 20 were bowhead whales. Additionally, preliminary microsatellite analyses of this specimen are not consistent with the hypothesis that whaling resulted in the low genetic variation found in this species today. These results differ from what would be expected based on any previous view of Basque whaling, and raise questions regarding the impact of Basque whaling on this species.

We examined six historical specimens of the endangered North Atlantic right whale (Eubalaena glacialis) using DNA isolated from documented baleen plates from the late nineteenth and early twentieth centuries. Sequences from the mitochondrial DNA (mtDNA) control region from these samples were compared with those from a near-exhaustive survey (269 of approximately 320 individuals) of the remaining right whales in the western North Atlantic Ocean. Our results suggest that there has been only relatively modest change in maternal lineage diversity over the past century in the North Atlantic right whale population. Any significant reduction in genetic variation in the species most likely occurred prior to the late nineteenth century. One historical specimen was from the last documented female capable of propagating one of the maternal lineages in the population today; no females in the existing population have been found to carry this mtDNA haplotype. Analysis of the only specimens from the eastern North Atlantic right whale population ever to be examined revealed that eastern and western North Atlantic right whales may not have been genetically differentiated populations. Loss of gene diversity experienced by North Atlantic right whales over the last century has been modest, and the six decades of protection have been successful in maintaining much of the maternal lineage diversity that was present in the late nineteenth century. Resumen: Examinamos seis especimenes historicos de la ballena franca septentrional Eubalaena glacialis del Atlantico Norte, usando ADN aislado de placas de las barbas documentadas de finales de siglo diecinueve y principios del siglo veinte. Las secuencias del ADN mitocondrial (mtADN) de la region control de estas muestras comparadas con aquellas de una muestra casi exhaustiva (269 de aproximadamente 320 individuos) de las ballenas francas remanentes en el oceano Atlantico Norte Occidental. Nuestros resultados sugieren que solo ha habido ligeros cambios en la diversidad del linaje materno a lo largo del siglo pasado. Probablemente, cualquier reduccion en la variacion genetica en las especies ocurrio antes del final del siglo diecinueve. Uno de los especimenes historicos fue de la ultima hembra documentada capaz de propagar uno de los linajes maternos en la poblacion actual. Ninguna de las hembras en las poblaciones existentes ha sido identificada como portadora de este haplotipo de mtADN. El analisis de los especimenes de la poblacion de ballenas del Atlantico Norte Oriental examinados revelan que las ballenas francas del este y del oeste del Atlantico Norte podrian no haber sido poblaciones geneticamente diferentes. La perdida de diversidad genetica experimentada en las poblaciones de ballena franca del Atlantico Norte a lo largo del siglo pasado ha sido modesta y las seis decadas de proteccion han sido exitosas en el mantenimiento de la mayoria de la diversidad del linaje materno que estaba presente a finales del siglo diecinueve.

Few studies have examined systematic relationships of right whales (Eubalaena spp.) since the original species descriptions, even though they are one of the most endangered large whales. Little morphological evidence exists to support the current species designations for Eubalaena glacialis in the northern hemisphere and E. australis in the southern hemisphere. Differences in migratory behaviour or antitropical distribution between right whales in each hemisphere are considered a barrier to gene flow and maintain the current species distinctions and geographical populations. However, these distinctions between populations have remained controversial and no study has included an analysis of all right whales from the three major ocean basins. To address issues of genetic differentiation and relationships among right whales, we have compiled a database of mitochondrial DNA control region sequences from right whales representing populations in all three ocean basins that consist of: western North Atlantic E. glacialis, multiple geographically distributed populations of E. australis and the first molecular analysis of historical and recent samples of E. glacialis from the western and eastern North Pacific Ocean. Diagnostic characters, as well as phylogenetic and phylogeographic analyses, support the possibility that three distinct maternal lineages exist in right whales, with North Pacific E. glacialis being more closely related to E. australis than to North Atlantic E. glacialis. Our genetic results provide unequivocal character support for the two usually recognized species and a third distinct genetic lineage in the North Pacific under the Phylogenetic Species Concept, as well as levels of genetic diversity among right whales world-wide.

DNA sequences of the mitochondrial control region of 180 North Atlantic right whales (Eubalaena glacialis) and 16 South Atlantic right whales (E. australis) have been determined using a combination of direct DNA sequencing and single stranded conformation polymorphism (SSCP) analysis. Five haplotypes were found in E. glacialis, and 10 in E. australis, but none were shared, supporting the reproductive isolation and separate species status of the North and South Atlantic right whales. One haplotype in E. glacialis was found in only three males born before 1982 and this matriline will likely be lost soon. The nucleotide diversity estimates for the five North Atlantic right whale haplotypes was 0.6% and 2.0% for the 10 haplotypes found in the South Atlantic right whales. The average haplotypic diversity was 0.87 in E. glacialis and 0.96 in E. australis, which is consistent with other studies showing a lower level of genetic variation in the North Atlantic right whale. Phylogenetic analysis identified two major assemblages of haplotypes in E. australis from the samples collected from Peninsula Valdes, suggesting a mixing of two historically divergent populations. Using genetic distance measurements with a divergence rate of 0.5%-1.0%/myr, we estimate E. glacialis diverged from E. australis 3-12.5 mya.

The North Atlantic right whale (Eubalaena glacialis) inhabits five areas along the east coast of North America at different times of the year. During 17 years of field observations, it has been found that only 59% of the newborn calves are brought to the Bay of Fundy nursery area. To examine whether this is because of population structuring, the mitochondrial DNA (mtDNA) control region haplotypes were determined for 269 individuals. Seven polymorphic sites were found to define five haplotypes. Calves were divided into two groups based on whether or not they were brought to the Bay of Fundy nursery. Significant genetic structuring of mitochondrial haplotypes was found between these groups (p = 0.002). Mothers that have had more than three calves demonstrated significant fidelity (p = 0.005) to the Bay of Fundy, suggesting that philopatry is the basis for the genetic structuring. Although this study clearly identifies the existence of two subpopulations and an alternative nursery area(s), the location(s) of the alternative area(s) is unclear and remains an important issue for the conservation of the species.

The North Atlantic right whale (Eubalaena glacialis) is the most endangered large whale in the world and shows few signs of recovery despite over 60 years of protection. In contrast, the South Atlantic right whale (E. australis), a closely related species, appears to be recovering successfully. Using DNA extracted from skin biopsy samples and two hypervariable minisatellite probes, we compared the levels of genetic diversity within the two populations. Our results revealed that unrelated North Atlantic (NA) right whales exhibit significantly less genetic variation than unrelated South Atlantic (SA) right whales (Jeffreys 33.15 (J33.15) probe: SNA-U-observed = 0.56 versus SSA-U-observed = 0.37, p NA-U-observed = 0.74 versus SSA-U-observed = 0.46, p NA-U-observed = 56 versus SSA-2°-expected = 0.53, p NA-U-observed = 0.74 versus SSA-2°-expected = 0.60, p NA-1°-observed = 0.67 versus SNA-1°-expected = 0.78, p NA-1°-observed = 0.83 versus SNA-1°-expected = 0.87, p = 0.15). This suggests that the matings between closely related individuals that would have resulted in the higher band-sharing values were unsuccessful. These results, in conjunction with behavioral and population data which indicate that North Atlantic right whales may be suffering from reduced fertility, fecundity, and juvenile survivorship, support the hypothesis that inbreeding depression is influencing the recovery of this species.

In the past decade, much progress has been made in real-time passive acoustic monitoring of marine mammal occurrence and distribution from autonomous platforms (e.g., gliders, floats, buoys), but current systems focus primarily on a single call type produced by a single species, often from a single location. A hardware and software system was developed to detect, classify, and report 14 call types produced by 4 species of baleen whales in real time from ocean gliders. During a 3-week deployment in the central Gulf of Maine in late November and early December 2012, two gliders reported over 25,000 acoustic detections attributed to fin, humpback, sei, and right whales. The overall false detection rate for individual calls was 14%, and for right, humpback, and fin whales, false predictions of occurrence during 15-min reporting periods were 5% or less. Transmitted pitch tracks--compact representations of sounds--allowed unambiguous identification of both humpback and fin whale song. Of the ten cases when whales were sighted during aerial or shipboard surveys and a glider was within 20 km of the sighting location, nine were accompanied by real-time acoustic detections of the same species by the glider within ±12 h of the sighting time.

Although small populations are expected to lose genetic diversity through genetic drift and inbreeding, a number of mechanisms exist that could minimize this genetic decline. Examples include mate choice for unrelated mates and fertilization patterns biased toward genetically dissimilar gametes. Both processes have been widely documented, but the long-term implications have received little attention. Here, we combined over 25 years of field data with high-resolution genetic data to assess the long-term impacts of biased fertilization patterns in the endangered North Atlantic right whale. Offspring have higher levels of microsatellite heterozygosity than expected from this gene pool (effect size = 0.326, P < 0.011). This pattern is not due to precopulatory mate choice for genetically dissimilar mates (P < 0.600), but instead results from postcopulatory selection for gametes that are genetically dissimilar (effect size = 0.37, P < 0.003). The long-term implication is that heterozygosity has slowly increased in calves born throughout the study period, as opposed to the slight decline that was expected. Therefore, this mechanism represents a natural means through which small populations can mitigate the loss of genetic diversity over time.

To test hypotheses involving reproduction and demographics, the sex of individuals must be established, but many species of Cetacea are not obviously dimorphic. In the North Atlantic right whale, Eubalaena glacialis, population, the sex of 61 males and 55 females had been determined previously by observation of the urogenital region, and the sex of 43 more females had been inferred from repeated sightings with a calf. To confirm the sex of some of these animals and to identify the sex of mote animals, genomic DNA was isolated from skin samples of 95 individual right whales (54 from among those described above and 41 additional recognizable individuals). The DNA was surveyed using the human Y-chromosome probe pDP1007. With EcoRI-digested DNA, a clear, sex-discriminating banding pattern was apparent. This method verified the sex of all 54 animals whose sex was previously known or inferred and identified the sex of an additional 41 recognizable individuals. A total of 89 male and 111 female right whales was identified in the population. The most unbiased estimate of sex ratio available is the 36 male and 34 female calves identified by genital morphology and DNA techniques. The sex ratio of this sample does not differ significantly from unity (P = 0.811). Only 38% (58/152) of the females in the North Atlantic population are known to have been reproductively successful compared with 54% in the population of right whales in the western South Atlantic. The population growth rate reported for the North Atlantic population is only 33% of that reported for right whales in the South Atlantic. Thirteen adult North Atlantic females have been identified that have not been known to calve during the past 11 yr. These data suggest that the absence of measurable recovery may be due to a combination of fewer actively reproducing females and lower reproductive rates of some females.

Few methods are available for determining the sex of free-ranging individual whales, dolphins, and porpoises of species that are not obviously sexually dimorphic. We have developed a technique for sexing beluga whales (Delphinapterus leucas) by using a Y-chromosome-specific DNA restriction fragment. Genomic DNA was extracted from liver samples of 18 beluga whales (9 males, 9 females) sexed at dissection. DNA from males and females was digested with five restriction enzymes, electrophoresed, and transferred to membranes by Southern blotting. When probed with the labelled human Y-chromosome zinc finger protein gene probe pDP1007, male-specific bands and bands common to both sexes, but more intense in females than in males, were observed. The DNA digested with EcoRI provided the clearest sex-discriminating banding pattern. Even when DNA of various qualities digested with EcoRI was used, all the males showed a 3.4-kilobase (kb) band, presumably from the Y-chromosome, as well as a 2.1-kb band. Females showed the 2.1-kb band, but all lacked the 3.4-kb band. This 3.4-kb EcoRI male-specific band permits unambiguous sex determination, which will facilitate examination of sex-related differences in population structure and habitat use of belugas, which have important implications for management decisions.

Parentage analyses of baleen whales are rare, and although mating systems have been hypothesized for some species, little data on realized male reproductive success are available and the patterns of male reproductive success have remained elusive for most species. Here we combine over 20 years of photo-identification data with high-resolution genetic data for the majority of individual North Atlantic right whales to assess paternity in this endangered species. There was significant skew in male reproductive success compared to what would be expected if mating was random (P < 0.001). The difference was due to an excess of males assigned zero paternities, a deficiency of males assigned one paternity, and an excess of males assigned as fathers for multiple calves. The variance in male reproductive success was high relative to other aquatically mating marine mammals, but was low relative to mammals where the mating system is based on resource- and/or mate-defence polygyny. These results are consistent with previous data suggesting that the right whale mating system represents one of the most intense examples of sperm competition in mammals, but that sperm competition on its own does not allow for the same degree of polygyny as systems where males can control access to resources and/or mates. The age distribution of assigned fathers was significantly biased towards older males (P < 0.05), with males not obtaining their first paternity until approximately 15 years of age, which is almost twice the average age of first fertilization in females (8 years), suggesting that mate competition is preventing younger males from reproducing. The uneven distribution of paternities results in a lower effective population size in this species that already has one of the lowest reported levels of genetic diversity, which may further inhibit reproductive success through mate incompatibility of genetically similar individuals.

The survival of North Atlantic right whales ( Eubalaena glacialis ) is seriously threatened by mortality caused by ships and entanglements in fishing gear. Demographic modeling indicates that the population is declining despite efforts to reduce anthropogenic mortalities, and in spite of recent increases in calving. The authors of this Policy Forum recommend immediate emergency management actions to reduce shipping and entanglement mortalities in right whales, so as to avoid a catastrophic population decline and inevitable extinction.

During the 16th and 17th centuries, Basque whalers travelled annually to the Strait of Belle Isle and Gulf of St. Lawrence to hunt whales. The hunting that occurred during this period is of primary significance for the North Atlantic right whale, Eubalaena glacialis (Müller, 1776), because it has been interpreted as the largest human-induced reduction of the western North Atlantic population, with ~12250–21 000 whales killed. It has been frequently reported that the Basques targeted two species in this region: the North Atlantic right whale and the bowhead whale, Balaena mysticetus L., 1758. To evaluate this hypothesis and the relative impact of this period of whaling on both species, we collected samples from 364 whale bones during a comprehensive search of Basque whaling ports from the 16th to the 17th century in the Strait of Belle Isle and Gulf of St. Lawrence. Bones were found and sampled at 10 of the 20 sites investigated. DNA was extracted from a subset (n = 218) of these samples. Analysis of the mitochondrial cytochrome b region identified five whale species. The identification of only a single right whale bone and 203 bowhead whale bones from at least 72 individuals indicates that the bowhead whale was likely the principal target of the hunt. These results imply that this whaling had a much greater impact (in terms of numbers of whales removed) on the bowhead whale population than on the western North Atlantic right whale population.

Polychlorinated biphenyls (PCBs), total DDT (DDT + DDE + DDD), dieldrin, heptachlor epoxide, chlordanes, and hexachlorobenzene (HCB) were found in blubber biopsies from endangered North Atlantic right whales (Eubalaena glacialis) in the Bay of Fundy and on Browns-Baccaro Banks. Analyses included four sex and age class composite samples from 1988, and 21 individual samples from 1989. Generally, PCBs demonstrated the highest wet weight residue levels (up to 1.9 micrograms/g), followed by total DDT (DDT + DDE + DDD) (trace to 0.47 micrograms/g). Relatively low residue levels in adult females suggest that transmammary organochlorine (OC) residue transfer occurs during lactation. The actual blubber residue loads may have been underestimated, because the samples were taken when the whales were depositing fat reserves and the samples may not have been representative of the remainder of the blubber.