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Dietary assessment plays a crucial role in comprehending the ecological dynamics and nutritional needs of herbivores and omnivores. The metabarcoding technique has emerged as a potent tool for exploring the dietary composition of these animals. However, various metabarcoding techniques have been developed, each with its own advantages and limitations. This study aims to compare the performance of different metabarcoding techniques in herbivores and omnivores diet. We systematically reviewed 159 published manuscripts in Scopus and Google Scholar, and thematic analysis was conducted across several categories, including the marker, platform, and database utilized. Preliminary findings reveal significant variations among metabarcoding techniques across these two animal groups. The trnL gene exhibited higher taxonomic resolution for herbivorous species, whereas the combination of the 'trnL + 16s rRNA' gene exhibited superior performance for omnivorous species. The Illumina platform emerged as the most commonly used method for analyzing the diets of both herbivores and omnivores, with the primary reference database being the National Centre for Biotechnology Information (NCBI). This study offers valuable insights into the strengths and limitations of different metabarcoding techniques for dietary assessment in herbivores and omnivores and optimizing metabarcoding protocols, facilitating more precise and reliable diet analyses within these ecological groups.

Gut microbiome studies have gained significant attention in recent years due to their potential in unveiling the role of microbial communities in animals’ health and ecological processes. However, the lack of standardized protocols in sample handling and processing across studies introduces variability, impeding the comparability of findings. This study addresses this issue by examining methodological variations in gut microbiome research on wildlife and domesticated animals in Southeast Asia. A comprehensive search of 91 relevant studies on the SCOPUS database yielded 54 suitable publications for review, encompassing diverse taxa such as invertebrates (20), fishes (7), reptiles (3), birds (5), and mammals (19). Notably, various methodological approaches were employed to characterize microbial communities, including the source of isolation, various culture-based approaches, sequencing methods, and the targeted markers. Based on the information provided in this study, future studies should strive to develop guidelines and best practices specific to gut microbiome studies. This would enhance comparability and facilitate the integration of findings. Such efforts will also advance our understanding of the microbial diversity associated with wildlife, and its potential implications for their health and conservation.

Next-generation sequencing (NGS) has transformed genomics, presenting significant potential for advancing wildlife conservation. NGS technology offers opportunities and challenges for addressing scientific questions in wildlife management. Unfortunately, there is a lack of comprehensive records on NGS implementation in Southeast Asia, particularly concerning wildlife conservation. To address this gap, we analyzed NGS studies focused on wildlife monitoring in Southeast Asia and introduced a practical framework for implementing NGS technologies in global wildlife conservation, especially in Southeast Asia. We systematically reviewed NGS studies in wildlife monitoring from the SCOPUS database. We identified 137 relevant publications from 11 countries, with Malaysia contributing 36% of the studies. The included studies were categorized into five themes: species identification, dietary assessment, health monitoring, taxonomic resolution, and whole-genome sequencing, with the majority focusing on 38 publications related to wildlife health. The framework developed in this study help the researchers and conservation practitioners with insights on NGS technology application in conservation while also addressing the benefits, limitations, and ethical considerations associated with NGS use. This review offers a brief overview of NGS usage and provides guidelines for embracing NGS as a valuable tool for effective wildlife conservation strategies in our rapidly changing world.

The Asian region is globally renowned for its unparalleled biodiversity, rich ecosystems, and unique cultural heritage. However, the delicate equilibrium between human societies and the diverse flora and fauna faces increasing challenges due to the region's rich biodiversity and escalating utilization of wildlife resources. Hence, this paper aims to document the utilization of endangered wildlife across Asian countries and to report the existing conservation measures and recommendations for sustainable wildlife management over the years. We employed prominent academic databases, specifically SCOPUS, to explore the dynamics of wildlife utilization and its associated implications. From the findings, 9,989 records were successfully identified. However, only 65 articles were included in the review after the screening process. A total of 61 species, comprising 30 mammals, 22 reptiles, one amphibian, and eight birds, all endangered, were identified as subjects of wildlife utilization in 16 Asian countries. China possessed the highest count for wildlife utilization across all categories, including 15 species used for trading purposes. In conclusion, this research underscores the critical need for integrated approaches that balance human needs and conservation imperatives to ensure a sustainable future for Asia's wildlife biodiversity.

The Bornean banded langur (Presbytis chrysomelas chrysomelas) is critically endangered species primarily found in Sarawak, Malaysia. Albeit this species is in peril, the ecology knowledge of this endemic species of Borneo is still scarce. Thus, a rapid survey employing total count and scan sampling method was conducted between July to August 2023 at Tanjung Datu National Park (TDNP), Sarawak to observe the social interaction of species with the environment. The behaviour of langur was recorded by employing scanning sampling method at 10 minutes intervals. This study sought to provide preliminary data on behavioural ecology of the Bornean banded langur within the national park. During the survey, three groups (consisting two to seven individuals) and a solitary male Bornean banded langur were recorded. The langurs were observed in both dipterocarp forests and coastal forests within the park. The daily activities of the Bornean banded langurs in TDNP were predominantly resting (31%), moving (29%), feeding (26%), vocalizing (14%), but not engaging in other social activities such as grooming, playing and mating. Knowing the behavioural ecological status as well as understanding ecology by identifying the activity pattern of langur is essential to government authorities and pertinent stakeholders to implement conservation strategies for the Bornean banded langur and their habitats. [ABSTRACT FROM AUTHOR]

Understanding the prey preference of Malayan tiger (Panthera tigris jacksoni) in Malaysia is important to guide conservation planning initiatives. The utilisation of DNA metabarcoding provides valuable insights, particularly in the field of carnivora diet research. This technique has been proven to be effective for identifying various species within complex mixtures such as scat materials, where visual identification is challenging. The Cytochrome c oxidase subunit I (COI) locus has been selected as it is a widely used as an effective non-invasive approach for diet studies. Hence, given this advance approach, Malayan tiger scats were collected on the basis of existing records of their presence in two types of habitats, namely, protected areas (PA) and human–tiger conflict (HTC) areas. This study aimed to identify prey species in Peninsular Malaysia, based on Malayan tiger scat samples using DNA metabarcoding. Based on the partial mitochondrial COI region, DNA metabarcoding led to the taxonomic resolution of prey DNA remnants in scats and the identification of prey species consumed by Malayan tiger, which were predominately small-to-medium-sized prey, including livestock. The dominant DNA prey detected belongs to the family Canidae, followed by Bovidae, Vespertilionidae, Homonidae, Felidae, Phasianidae and Muridae. A significant difference (p < 0.05) was observed in alpha and beta diversity using the Shannon index and PERMANOVA with regard to prey richness and evenness in two different habitat groups, namely, PA and HTC. Our finding provides insights into Malayan tiger dietary requirements, which can be used to develop conservation plans and strategies for Malayan tiger, particularly for habitat priorities. [ABSTRACT FROM AUTHOR]

Tarsier is an endangered nocturnal primate in the family Tarsiidae and is an endemic to Sundaic islands of Philippine (Carlito syrichta), Sulawesi (Tarsius tarsier-complex) and Borneo (Cephalopachus bancanus). Recent records indicated that most molecular studies were done on the Eastern Tarsier and little information for the other group of tarsiers. Here, we present a partial cytochrome b data set of C. bancanus in Sarawak, Malaysian Borneo. Standard mist nets were deployed at strategic locations in various habitat types. A total of 18 individuals were caught, measured and weighed. Approximately, 2 × 2 mm of tissue samples were taken and preserved in molecular grade alcohol. Out of 18, only 11 samples were screened with partial mtDNA (cytochrome b) and the DNA sequences were registered in the GenBank (accession numbers: KY794797-KY794807). Phylogenetic trees were constructed with 20 additional mtDNA sequences downloaded from GenBank. The data are valuable for the management authorities to regulate the type of management units for the metapopulation to sustain population genetics integrity of tarsiers in the range countries across the Sunda Shelf. Keywords: Cytochrome b gene, Population genetics, Primate, Tarsier, Borneo

The critically endangered Bornean banded langur (Presbytis chrysomelas) is exclusively found in Borneo. This species is classified among the world's 25 most endangered primate species. The geographical locations and distribution patterns of P. chrysomelas remain inadequately understood, and their taxonomic classification remains unclear. Although it was once abundant in Sarawak, the distribution of P. chrysomelas has become one of the most restricted among all Presbytis species. Presently, documented sightings of P. chrysomelas span across five specific locations in Sarawak: Samunsam Wildlife Sanctuary, Tanjung Datu National Park, Gunung Pueh National Park, Similajau National Park, and Maludam National Park. Thus, the purpose of this review is to present previous studies conducted on P. chrysomelas in Sarawak, elucidating its taxonomy, characteristics, distributions, important sites, research gaps, threats, and recommendations for further studies. Immediate conservation efforts and attention from the primatologist, relevant authorities and stakeholders are needed to prevent the localized extinction of P. chrysomelas. [ABSTRACT FROM AUTHOR]

Sarawak is home to a diverse array of primate species in Malaysia, i.e., 14 species from five families. Over the years, many studies on primates’ behavior, ecology and conservation have been conducted in Sarawak. Here, we provide a comprehensive overview of previous primate studies in Sarawak from 1960 to 2022 and discuss primates' threats, conservation status, and initiatives. Despite the vast number of these primate studies, the majority of them only focus on proboscis monkeys (n=24) and orangutans (n=21) and lack comprehensive studies that cover all primate species in Sarawak. Studies on ecology and natural history were the most prevalent for all Sarawak primate genera (n=51), accounting for more than half of publications. Thus, it advocates a more holistic approach to fill the knowledge gaps and meet conservation needs. Conservation efforts are urgently needed to protect primates currently threatened by deforestation, habitat fragmentation, hunting, and illegal trade. However, conservation efforts may be restrained by the limited information on primates in Sarawak. To preserve the primates in Sarawak, a robust protection strategy that synergizes the participation of government authorities, non-government organizations, and local communities needs to be developed. [ABSTRACT FROM AUTHOR]

Long-tailed macaques have been roaming near Tenaga National Berhad Bukit Selambau Solar (TBSS) causing human–macaque conflicts. This study reveals the social organization and genetic variation of these macaques. Macaques’ groups were determined via direct observation and closedcircuit television. Genomic DNA from 29 fecal samples were extracted and proceeded with amplification of the D-loop region of mitochondrial DNA. There are four main groups of primates at TBSS. Some members of the Kuil group and the Pekan group shared haplotype 1, forming a fission-fusion society. This finding can be used for TNB in mitigation plans involving human– macaque conflict. [ABSTRACT FROM AUTHOR]

The Red, Green and Blue (RGB) colour model has been used to investigate relationships between primates' physiological and colour data. This study uses the RGB method to determine various pelage hues in white-thighed surili at different latitudes in Peninsular Malaysia. Universiti Kebangsaan Malaysia (UKM) represents the lowland while Genting Highlands and Fraser’s Hill represent the highland area. Results indicated that no significant values were found based on the sample t-test on every section of the samples except on the nose (Green). Our findings can be utilised further for systematic and population genetic studies of Presbytis siamensis siamensis in Peninsular Malaysia. [ABSTRACT FROM AUTHOR]