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BACKGROUND Differentiating prostate carcinoma (PCa) arising in the neck of urinary bladder from high grade urothelial cancer (UCa) with prostatic extension can be a difficult task for histopathologist due to similar morphologic characteristics and overlapping clinical manifestations in the two diseases. These two tumours often occur in association with one another but have different potential therapeutic strategies and prognostic implications. We have investigated p63 immunohistochemical (IHC) marker as simple first line marker adjuvant to histopathological examination. METHODS In this prospective study, total 50 cases including 25 cases of urothelial carcinoma and 25 cases of prostatic carcinoma were taken. Tumour grade was determined according to standard H&E staining and scoring system. p63 expressions were determined by immunohistochemical staining of all the cases. The obtained results were analysed and evaluated using chi-square statistical test to determine whether p63 IHC can be used as simple first line marker tool with a high sensitivity and specificity. RESULTS p63 was not expressed in any of the 25 cases of prostatic carcinoma cases while in urothelial carcinoma it was expressed in 23 of 25 (92 %) cases. p63 IHC staining expression is positive in all histological grades of urothelial carcinomas. 2 out of 25 cases of urothelial carcinomas were negative for p63 IHC expression. None of the prostatic adenocarcinomas expressed p63 staining. Sensitivity of p63 stain in differentiating UCa with PCa was 92 % in our study, specificity of p63 stain in differentiating UCa with PCa was found to be 100 %. CONCLUSIONS p63 can be used as a screening first line IHC marker to distinguish urothelial carcinomas from prostatic adenocarcinomas. For challenging and unresolved cases both of these have limited sensitivity; thus, authors recommend two lineagespecific markers one each for UCa (GATA3, S100P) and PCa (NKX3.1, P501S, PSMA) should be used for definitive diagnosis. KEYWORDS p63 Immunohistochemistry, Urothelial Carcinoma, Prostatic Adenocarcinoma

Melanotic neuroectodermal tumor of infancy (MNTI) is a rare pigmented neoplasm of neural crest origin that most commonly arises in the maxilla of neonates and infants during the first year of life. We report the case of a three-month-old female child who presented with a 15-day history of intraoral swelling. Radiologically, the tumor was detected in the right maxilla, which was removed by wide local excision. Histopathological examination revealed the biphasic population of cells with melanin pigment deposition. Immunohistochemistry study was done confirming the diagnosis of MNTI.

Fibroadenoma, being a very common benign tumor of the breast in young females, does not pose any threat and, thus, can be treated with lumpectomy. Breast cancer arising within a fibroadenoma is a rare phenomenon, but detecting these neoplasms is of utmost importance for complete treatment and follow-up. These lesions are an incidental finding in a lumpectomy specimen done for fibroadenoma breast. A 28-year-old female presented with multiple bilateral lumps for eight years. The lumps were mobile, non-tender, and slowly growing, with no nipple discharge, no axillary lymphadenopathy, and no family history. The diagnosis of a benign lesion suggestive of fibroadenoma was given on ultrasonography (USG) and fine-needle aspiration cytology (FNAC). The patient underwent lumpectomy and the excised tissues were subjected to histopathological examination. Grossly, multiple, well-circumscribed, encapsulated masses, with the largest measuring 4x2.5x2 cm were noted. All masses showed homogenous grey-white areas with slit-like spaces. On microscopy, predominant areas were consistent with fibroadenoma, with few foci showing the features of ductal carcinoma in situ (DCIS) with nests of cells having pleomorphic nuclei, prominent nucleoli with atypical mitosis, a cribriform pattern, and comedo necrosis. This highlights the rare association of fibroadenoma and carcinoma in situ, thus, a careful and extensive sampling of the tissue is required to prevent the false negative diagnosis by pathologists.