Search

Minerals play an essential role in the normal vital process. Calcium, phosphorus and magnesium are the most abundant minerals. Vitamin D and parathyroid hormone play a key role in regulation of their homeostasis as well. The aim of this study was to evaluate calcium, phosphorus, magnesium, vitamin D and parathyroid hormone on 30 Caspian horses of Guilan Province (south of the Caspian Sea) in two different seasons. So that, 15 stallions and 15 mares were sampled at the age groups ≤ 3 years and > 3 years, respectively. Fasting blood samples were collected twice, in August and January 2015 from jugular vein of horses. Serum calcium, phosphorus and magnesium were measured by colorimetric methods, while 25(OH)D3 and parathyroid hormone were measured by ELISA. The calcium (11.50 vs. 14.25 mg dL-1 ), magnesium (2.13 vs. 3.72 mg dL-1 ) and vitamin D (1.66 vs. 2.48 ng mL-1 ) levels were lower in winter than in summer (P < 0.05). The Caspian horses had higher phosphorus (4.52 vs. 3.26 mg dL-1 ) in winter than in summer (P < 0.05). Effect of sex on the measured parameters was not significant. Effects of age on the calcium, magnesium, vitamin D and parathyroid hormone levels were not significant, but ≤ 3 year - old horses had higher phosphorus (4.63 vs. 3.15 mg dL-1 ) than > 3 year - old ones (P < 0.05). The vitamin D level of mares was higher (3.10 vs. 1.43 ng mL-1 ) in summer than in winter (P < 0.05). Effects of sex, season, age and their interactions on parathyroid hormone were not significant. In conclusion, calcium, phosphorus, magnesium and parathyroid hormone levels in Caspian horses were within their physiological range, but vitamin D was low without any signs of deficiency. The Caspian horses had lower calcium, magnesium and vitamin D levels in winter than in summer.

The current paradigm of medicine is mostly designed to block or prevent pathological events. Once the disease-led tissue damage occurs, the limited endogenous regeneration may lead to depletion or loss of function for cells in the tissues. Cell therapy is rapidly evolving and influencing the field of medicine, where in some instances attempts to address cell loss in the body. Due to their biological function, engineerability, and their responsiveness to stimuli, cells are ideal candidates for therapeutic applications in many cases. Such promise is yet to be fully obtained as delivery of cells that functionally integrate with the desired tissues upon transplantation is still a topic of scientific research and development. Main known impediments for cell therapy include mechanical insults, cell viability, host's immune response, and lack of required nutrients for the transplanted cells. These challenges could be divided into three different steps: 1) Prior to, 2) during the and 3) after the transplantation procedure. In this review, we attempt to briefly summarize published approaches employing biomaterials to mitigate the above technical challenges. Biomaterials are offering an engineerable platform that could be tuned for different classes of cell transplantation to potentially enhance and lengthen the pharmacodynamics of cell therapies.

This study is an attempt to evaluate the biomonitoring capabilities of rock oyster, Saccostrea cucullata, for mercury (Hg) pollution. The oyster and sediment samples were collected from 10 rocky habitats of Qeshm and Hormoz Islands in the Persian Gulf. The concentration of mercury in the shell and soft tissues of the oysters and sediments were analyzed using an advanced mercury analyzer. Biota-sediment accumulation factor (BSAF) was calculated based on the ratio of Hg concentrations in soft tissues to that in sediments. The results showed that the rate of mercury accumulation in the soft tissues of the oyster was significantly higher than that in its shell (P < 0.05). There was a significant correlation between mercury concentrations in the soft tissues and the sediments (r =0.75). According to BSAF, soft tissues of the oyster were recognized as an appropriate indicator for biomonitoring mercury. The present study generally supports the usability of soft tissue of S. cucullata as a sensitive biomonitoring organ to warn mercury pollution in the Persian Gulf.

Levels of contaminants in fish are of particular interest because of the potential risk to humans consuming them. This paper examines the levels of cadmium, lead, zinc and copper in liver and muscle tissue of Esox lucius and in sediments from the Anzali international wetland in Iran. The wetland is a nursery and feeding habitat for fish species both from the lagoon and the Caspian Sea, which are consumed by the local inhabitants and others. Eleven main rivers discharge agricultural, industrial, urban and municipal wastes into this wetland. Twenty five Esox lucius specimens, with a mean weight of 804.6±121g and mean length of 430.7± 2mm were collected from the wetland by multimesh gill nets in September and December 2007.. The age was determined from scale samples according to the annual ring structure. Average concentrations of Cd, Cu, Pb and Zn accumulated in the mid-dorsal muscle tissue (filleted and skinned) of fish were 0.001, 0.21±0.02, 0.13±0.01 and 2.55±0.18 µg.g-1, respectively. The concentrations of these metals in liver samples were Cd: 0.0014± Cu: 0.96± 0.61, Pb: 0.11 ± 0.04 and Zn: 2.46±1.5 µg.g-1. Significant positive correlations were detected between Zn and Cu with body weight, while negative correlations were found between Pb and body weight. The concentrations of Zn, Cu and Cd were below the maximum allowable concentrations for fish proposed by WHO and MAFF (safe for human consumption) but concentration of Pb exceeded the standard levels.

Foreign body response (FBR) to biomaterials compromises the function of implants and leads to medical complications. Here, we report a hybrid alginate microcapsule (AlgXO) that attenuated the immune response after implantation, through releasing exosomes derived from human Umbilical Cord Mesenchymal Stem Cells (XOs). Upon release, XOs suppress the local immune microenvironment, where xenotransplantation of rat islets encapsulated in AlgXO led to >170 days euglycemia in immunocompetent mouse model of Type 1 Diabetes. In vitro analyses revealed that XOs suppressed the proliferation of CD3/CD28 activated splenocytes and CD3+ T cells. Comparing suppressive potency of XOs in purified CD3+ T cells versus splenocytes, we found XOs more profoundly suppressed T cells in the splenocytes co-culture, where a heterogenous cell population is present. XOs also suppressed CD3/CD28 activated human peripheral blood mononuclear cells (PBMCs) and reduced their cytokine secretion including IL-2, IL-6, IL-12p70, IL-22, and TNFα. We further demonstrate that XOs mechanism of action is likely mediated via myeloid cells and XOs suppress both murine and human macrophages partly by interfering with NFκB pathway. We propose that through controlled release of XOs, AlgXO provide a promising new platform that could alleviate the local immune response to implantable biomaterials.