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A series of binuclear ruthenium(II) arene benzil bis(benzoylhydrazone) complexes (1–6) of the common composition [(η6-arene)2Ru2(L)Cl2] (arene = benzene or p-cymene; L = benzil bis(benzoylhydrazone derivatives)) have been synthesized. The synthesised complexes have been thoroughly characterised by elemental analysis, IR, NMR, UV-vis and ESI-MS spectral methods. The coordination of the binucleating ligand to each ruthenium centre through azomethine nitrogen and imidolate oxygen was confirmed by single crystal XRD, which indicates the presence of the pseudo octahedral geometry. All the complexes were carefully screened for their cytotoxicity against HeLa, MDA-MB-231 and Hep G2 cells under in vitro conditions. Interestingly, the complexes 4 and 6 exhibit higher cytotoxicity than cisplatin, with low IC50 values against all screened cancer cell lines. Furthermore, the mode of cell death in MDA-MB-231 cells was assessed by AO-EB, Hoechst 33258 staining and a flow cytometry technique along with the comet assay. The result of western blot analysis suggests that complexes 4 and 6 were shown to induce apoptosis via the mitochondrial pathway by up-regulating p53 and Bax, and down-regulating Bcl-2.

The infected diabetic wound ulcer is a significant problem for the diabetic patients, which leads to removal of affected foot site due to its delayed/non-healing tissues. The poly-microbial infections and active matrix metalloproteinases (MMP) are the significant influencing factors to delayed healing in diabetic mice. The main purposes of present investigation are to evaluation the targeted inactivation of MMP and avoid polymicrobial infections by using a combined therapeutic effect of metal chelating dipeptide (L-carnosine) and curcumin with the biocompatible silk protein hydrogel (L-car@cur/SF) dressing in the infected diabetic wound ulcer. The in vitro biological assay methods, such as, cell viability, anti-oxidant activity, anti-inflammatory macrophage cells and inhibition collagenase exhibited that the designed hydrogel matrix to be human cell compatible and could be accelerate for significant diabetic healing potential. The activation of cur/SF matrix by the L-carnosine was persuading the inactivation of matrix metalloproteinase-9 (MMP-9) through its potent chelating effects of Zn2+ ions from the MMP-9 active center. The L-car@cur/SF hydrogel was demonstrated for the effective MMP-9 inactivation and bacterial inhibition via in vivo mice wound site, which indorsed the diabetic wound healing efficiency in streptozotocin-tempted diabetic mice.