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Your search keyword '"Mohamed M. I. Salem"' showing total 2 results
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2 results on '"Mohamed M. I. Salem"'

1. A Single-Step Genome-Wide Association Study for Semen Traits of Egyptian Buffalo Bulls

Author

Ayman G. EL Nagar, Mohamed M. I. Salem, Amin M. S. Amin, Maher H. Khalil, Ayman F. Ashour, Mohammed M. Hegazy, and Hamdy Abdel-Shafy

Subjects
semen traits, X-chromosome, candidate genes, genomic selection, ss-GWAS, buffalo, Veterinary medicine, SF600-1100, Zoology, QL1-991
Abstract

The present study aimed to contribute to the limited research on buffalo (Bubalus bubalis) semen traits by incorporating genomic data. A total of 8465 ejaculates were collected. The genotyping procedure was conducted using the Axiom® Buffalo Genotyping 90 K array designed by the Affymetrix Expert Design Program. After conducting a quality assessment, we utilized 67,282 SNPs genotyped in 192 animals. We identified several genomic loci explaining high genetic variance by employing single-step genomic evaluation. The aforementioned regions were located on buffalo chromosomes no. 3, 4, 6, 7, 14, 16, 20, 22, and the X-chromosome. The X-chromosome exhibited substantial influence, accounting for 4.18, 4.59, 5.16, 5.19, and 4.31% of the genomic variance for ejaculate volume, mass motility, livability, abnormality, and concentration, respectively. In the examined genomic regions, we identified five novel candidate genes linked to male fertility and spermatogenesis, four in the X-chromosome and one in chromosome no. 16. Additional extensive research with larger sample sizes and datasets is imperative to validate these findings and evaluate their applicability for genomic selection.

Published
2023
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2. Expression profiling of HSP 70 and interleukins 2, 6 and 12 genes of Barki sheep during summer and winter seasons in two different locations

Author

Raed A. Abu Rawash, Mahmoud A. Sharaby, Gamal El-Din A. Hassan, Alaa E. Elkomy, Elsayed E. Hafez, Salma H. Abu Hafsa, and Mohamed M. I. Salem

Subjects
Atmospheric Science, Hot Temperature, Sheep, Ecology, Hydrocortisone, Interleukin-6, Health, Toxicology and Mutagenesis, Heat Stress Disorders, Interleukin-12, Glucose, Albumins, Animals, Interleukin-2, Female, HSP70 Heat-Shock Proteins, Seasons
Abstract

The objectives of this research were to contrast the expression values of heat shock protein (HSP70) and interleukins 2, 6 and 12 (IL 2, IL 6 and IL 12) genes in summer and winter in two different locations in Egypt (Alexandria zone and Matrouh zone) to deduce changes in thermo-physiological traits and biochemical blood metabolites of Barki sheep. A total of 50 ewes (20 in Alexandria and 30 in Matrouh) were individually blood sampled to determine plasma total protein (TP), Albumin, Globulin and Glucose constituents and T3, T4 and cortisol hormones. The thermo-physiological parameters of rectal temperature (RT, °C), skin temperature (ST, °C), Wool temperature (WT, °C), respiration rate (RR, breaths/min) and pulse rate (PR, beats/min) were measured for each ewe. Expressions of IL 2, IL 6, IL 12 and HSP 70 in summer and winter were analyzed along with thermo-physiological parameters and blood biochemical metabolites. In both locations, THI had significant effects on ST, WT, PR and RR, but not significant on RT. However, it had no significant effects on blood plasma metabolites and hormonal concentrations in the two locations in summer and winter. In Alexandria location, THI had negative significant effect on the expressions of IL-2 and IL-6 but positively affected on HSP70 genes in winter, while the expression of IL-12 gene was not affected by seasons, whereas in Matrouh zone, the effects of THI on the expressions of all tolerance genes were not significant. The results of the current study suggest that IL-2, IL-6 and HSP70 genes could be used as molecular markers for heat/cold stress.

Published
2021

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