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14. Myeloperoxidase and its products in synovial fluid of patients with treated or untreated rheumatoid arthritis

Author

Nzeusseu Toukap, A., primary, Delporte, C., additional, Noyon, C., additional, Franck, T., additional, Rousseau, A., additional, Serteyn, D., additional, Raes, M., additional, Vanhaeverbeek, M., additional, Moguilevsky, N., additional, Nève, J., additional, Vanhamme, L., additional, Durez, P., additional, Van Antwerpen, P., additional, and Zouaoui Boudjeltia, K., additional

Published
2014
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16. Native and recombinant proteins to analyze auto-antibodies to myeloperoxidase in pauci-immune crescentic glomerulonephritis

Author

Boomsma, MM, Stegeman, CA, Oost-Kort, WW, Kallenberg, CGM, Moguilevsky, N, Limburg, PC, Tervaert, JWC, Groningen Kidney Center (GKC), and Translational Immunology Groningen (TRIGR)

Subjects
myeloperoxidase, WEGENERS-GRANULOMATOSIS, ANCA, animal diseases, ANTINEUTROPHIL CYTOPLASMIC ANTIBODIES, SYSTEMIC VASCULITIS, AUTOANTIBODIES, cardiovascular diseases, recombinant, pauci-immune necrotizing crescentic glomerulonephritis, RAPIDLY PROGRESSIVE GLOMERULONEPHRITIS, THERAPY, CLASSIFICATION
Abstract

The prevalence of Anti-Neutrophil Cytoplasmic Antibodies (ANCA) directed against myeloperoxidase (MPO) in pauci-immune necrotizing crescentic glomerulonephritis (NCGN) is dependent on the assay(s) used, We investigated the frequency of MPO-ANCA as detected by different assays for MPO-ANCA in a large cohort of patients with biopsy-proven pauci-immune NCGN. Sera from 121 consecutive untreated patients presenting with pauci-immune NCGN were tested for ANCA directed to proteinase-3 (PR3) at diagnosis. PR3-ANCA negative sera were tested by direct ELISA using recombinant or native MPO and by capture ELISA using two different specific monoclonal antibodies directed to MPO and three different antigenic sources. Sera from 80 relevant disease controls were tested to explore the specificity of the different assays. Thirty-eight out of 121 patients (31%) with pauci-immune NCGN did not have PR3-ANCA. Sufficient amounts of serum from 30 of these 38 PR3-ANCA negative patients were available for further testing, Recombinant and native MPO were recognized by similar numbers of sera in a direct ELISA (recombinant MPO: 93%. native MPO: 93%) and a capture ELISA (recombinant MPO: 77-87%. native MPO: 93%). Sera of patients with PR3-ANCA positive pauci-immune NCGN and disease controls were less frequently positive for MPO-ANCA in a capture ELISA (recombinant MPO: 3-7%, native MPO: 6-7%) than in a direct ELISA (recombinant MPO: 25%, native MPO: 13%). Both direct and capture ELISA assays using either native or recombinant MPO are sensitive techniques to detect MPO-ANCA in patients with pauci-immune NCGN. A capture ELISA performs better than a direct ELISA because it combines a higher specificity with a comparable sensitivity. Recombinant MPO is a good alternative for native MPO when used as antigen in a capture ELISA, but not when used in a direct ELISA because of lower specificity in this latter assay. (C) 2001 Elsevier Science B.V. All rights reserved.

Published
2001

20. Antigens and granularity of blood monocytes in relation to inflammatory markers and lipids in postmenopausal women

Author

UCL - SC/BIOL - Département de biologie, Boudjeltia, K. Zouaoui, Gregoir, C., Guillaume, M., Remacle, Claude, Piro, P., Garbar, C., Ducobu, J., Moguilevsky, N., Vanhaeverbeek, M., Delrée, P., Brohee, D., UCL - SC/BIOL - Département de biologie, Boudjeltia, K. Zouaoui, Gregoir, C., Guillaume, M., Remacle, Claude, Piro, P., Garbar, C., Ducobu, J., Moguilevsky, N., Vanhaeverbeek, M., Delrée, P., and Brohee, D.

Abstract

Objectives: The menopause is associated with an increase of inflammatory markers (C-reactive protein, fibrinogen), cytokines (INF gamma, TNF, etc.) and blood lipoproteins. In vitro, CRP, LDL and fibrinogen can modulate or potentiate interleukines production by monocytes. The aim of this work was to study, the relationships in vivo between hs-CRP, fibrinogen, lipoproteins and the phenotype of circulating monocytes. Methods: The monocytes phenotype, in postmenopausal women (t.=26) without history of cardiovascular disease, was determined, by flow cytometry, measuring granularity and CD 14. HLA-DR and CD62-L antigens expression. Blood monocytes were divided in CD14+dim monocytes (low CD14 expression) and CD14+bright monocytes (high CD14 expression). Results: HLA-DR was negatively correlated with hs-CRP and fibrinogen. The relationships between ApoB, LDL/ApoB ratio and CD 14 expression was restricted to the CD 14+bright monocytes. Blood lipids, i.e. total cholesterol, LDL-c and ApoB were correlated with the granularity of both subsets. CD 14+dim monocytes were characterized by a low granularity and CD62-L expression. Conclusions: Our data show that fibrinogen and hs-CRP are correlated with a reduced antigen-presenting capacity. Expression of CD 14 on CD 14+bright monocytes is negatively associated to atherogenic LDL. Blood monocytes granularity was positively correlated with serum lipids indicating that monocytes could uptake modified LDL in circulation and not restricted to subendothelial space. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

Published
2006

21. Oxidation of low density lipoproteins by myeloperoxidase at the surface of endothelial cells: an additional mechanism to subendothelium oxidation

Author

UCL - SC/BIOL - Département de biologie, Boudjeltia, KZ, Moguilevsky, N., Legssyer, I, Babar, S., Guillaume, M., Delrée, P., Vanhaeverbeek, M., Brohee, D., Ducobu, J., Remacle, Claude, UCL - SC/BIOL - Département de biologie, Boudjeltia, KZ, Moguilevsky, N., Legssyer, I, Babar, S., Guillaume, M., Delrée, P., Vanhaeverbeek, M., Brohee, D., Ducobu, J., and Remacle, Claude

Abstract

The present paradigm of atherogenesis proposes that low density lipoproteins (LDL) are trapped in subendothelial space of the vascular wall where they are oxidized. Myeloperoxidase (MPO) plays a key role in oxidative damage. We propose that LDL oxidation by myeloperoxidase (Mox-LDL) could occur at the surface of the endothelial cells and not restricted to the subendothelial space. The triad constituted by endothelial cells, circulating LDL and MPO in close interaction, constitutes a synergic mechanism for the genesis of Mox-LDL. (C) 2004 Elsevier Inc. All rights reserved.

Published
2004

22. Hypotheses for a re-evaluation of peroxidase activity in the oral inflammation: NAD(P)H-dependent hypohalite reduction in human neutrophils

Author

Philippe Courtois, Vanden Abeele A, Moguilevsky N, Amrani N, and Pourtois M

Subjects
Stomatitis, Peroxidases, Neutrophils, Humans, NADH, NADPH Oxidoreductases, Oxidation-Reduction, Hypochlorous Acid
Abstract

The balance of NADH oxidation by neutrophil lysates in the presence and in the absence of peroxidase/H2O/halide systems, pointed out a NADH oxidation excess when these hypohalite generators were actually present. This unexplained NADH consumption could not be attributed to a direct oxidation by hypohalites nor by the lysate self. Using OCI- as substrate, the NADH oxidation excess ranged from 52 to 99 mU/ml at pH 7.40 and from 446 to 1346 mU/ml at pH 6.00 (N = 6). Similar results were also found while using hypoiodous and hypothiocyanous compounds at pH 7.40 but not at pH 5.50. The authors discuss different hypotheses to explain this phenomenon and accordingly elaborate on the possible consequences of neutrophils degranulation in the oral tissues during inflammation processes.

Published
1994

34. Uptake of recombinant myeloperoxidase, free or fused to Fc gamma, by macrophages enhances killing activity toward micro-organisms.

Author

UCL - MD/BICL - Département de biochimie et de biologie cellulaire, Tournay, C, Courtoy, Pierre J., Marodi, L, Totté, P, Werenne, J., Jacquet, A, Garcia-Quintana, L, Bollen, A., Moguilevsky, N., UCL - MD/BICL - Département de biochimie et de biologie cellulaire, Tournay, C, Courtoy, Pierre J., Marodi, L, Totté, P, Werenne, J., Jacquet, A, Garcia-Quintana, L, Bollen, A., and Moguilevsky, N.

Abstract

A chimeric antibody-like molecule consisting of the human myeloperoxidase (rMPO) fused to the second and third constant-sequence (CH2 and CH3) Fc domains of human immunoglobulin G-1 has been constructed and expressed in Chinese hamster ovary (CHO) cells. This fusion molecule was designed to combine the binding specificity of Fc with the antimicrobial properties of rMPO. The rMPO-Fc fusion dimerized through the Fc fragment, while retaining the enzymatic activity of rMPO. The chimeric molecule was glycosylated and most of the propeptide was eliminated, indicating a better processing of the polypeptide than for rMPO alone. Both rMPO and rMPO-Fc bound to and were internalized by macrophage-like U937 promonocytic cells. Unexpectedly, the chimera failed to bind to the Fc receptor but interacted with a higher affinity than rMPO with the same binding sites. The presence of the Fc fragment in the chimera, in addition, did not extend the plasma half-life of the fusion protein. In vitro, rMPO-Fc exhibited a stronger killing effect than rMPO toward Candida albicans in the presence of either H202 alone or human macrophages. In vivo, rMPO-Fc similarly conferred a better protection than rMPO in a lethal model of murine cowdriosis. These properties could be related to the Fc-induced dimerization of the fusion protein in CHO cells.

Published
1996

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