40 results on '"Moebius C"'
Search Results
2. Ösophaguschirurgie
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Möbius, C., Richter, Olaf, editor, and Uhlmann, Dirk, editor
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- 2018
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3. Magenchirurgie
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Möbius, C., Uhlmann, D., Richter, Olaf, editor, and Uhlmann, Dirk, editor
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- 2018
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4. INTEREST IN CD2, a global patient-centred study of long-term cervical dystonia treatment with botulinum toxin
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Misra, Vijay P., Colosimo, Carlo, Charles, David, Chung, Tae Mo, Maisonobe, Pascal, Om, Savary, Abdulnayef, A., Adatepe, N. U., Araujo Leite, M. A., Badarny, S., Bajenaru, O., Bares, M., Bejjani, P., Bergmans, B., Bhidayasiri, R., Bozic, H., Cardoso Costa, F. E., Carlstrom, C., Castelnovo, G., Chang, M. H., Chang, Y. Y., Coletti-Moja, M., Delvaux, V., Dioszhegy, P., Dogu, O., Duzynski, W., Ehler, E., Espinosa Sierra, L., Fabbrini, G., Ferreira, J., Ferreira Valadas, A., Foresti, C., Girlanda, P., Goh, K. J., Graca Velon, A., Grill, S., Gurevitch, T., Hadidi, M., Hamimed, M. A., Hamri, A., Harrower, T., Hassin, S., Hedera, P., Hernandez, J. F. J. G., Hernandez Franco, J., Ho, B., Ho, S. L., Hughes, A., Ilic, T., Inshasi, J. S., Ip, C. W., Jamieson, S., Jamora, R. D. G., Jech, R., Jeon, B. S., Kaminska, A., Karpova, M., Khasanova, D., Kim, J. M., Kim, J. W., Kok, C. Y., Korenko, A., Korv, J., Koussa, S., Kovacs, T., Kreisler, A., Krystkowiak, P., Kumthornthip, W., Lin, C. H., Lundin, F., Lus, G., Magalhaes, M., Masmoudi, A. N., Mercelis, R., Misbahuddin, A., Moebius, C., Mohammadi, B., Nazem, B., Ng, K., Nurlu, G., Nyberg, J., Nyholm, D., Ochudlo, S., Otruba, P., Pfister, R., Pirtosek, Z., Pokhabov, D., Quinones Aguilar, S., Quinones Canales, G., Raghev, S., Rickmann, H., Romano, M., Rosales, R. L., Rubanovits, I., Santilli, V., Schoels, L., Simonetta-Moreau, M., Simu, M. A., Sohn, Y. H., Soulayrol, S., Supe, I., Svetel, M., Sycha, T., Tan, E. K., Timerbaeva, S., Tokcaer, A. B., Trosch, R., Tugnoli, V., Tumas, V., van der Linden, C., Vetra, A., Vial, C., Vidry, E., Williams, D., Wimalaratna, S., Yiannikas, C., Misra, Vijay P., Colosimo, Carlo, Charles, David, Chung, Tae Mo, Maisonobe, Pascal, Om, Savary, Abdulnayef, A., Adatepe, N. U., Araujo Leite, M. A., Badarny, S., Bajenaru, O., Bares, M., Bejjani, P., Bergmans, B., Bhidayasiri, R., Bozic, H., Cardoso Costa, F. E., Carlstrom, C., Castelnovo, G., Chang, M. H., Chang, Y. Y., Coletti-Moja, M., Delvaux, V., Dioszhegy, P., Dogu, O., Duzynski, W., Ehler, E., Espinosa Sierra, L., Fabbrini, G., Ferreira, J., Ferreira Valadas, A., Foresti, C., Girlanda, P., Goh, K. J., Graca Velon, A., Grill, S., Gurevitch, T., Hadidi, M., Hamimed, M. A., Hamri, A., Harrower, T., Hassin, S., Hedera, P., Hernandez, J. F. J. G., Hernandez Franco, J., Ho, B., Ho, S. L., Hughes, A., Ilic, T., Inshasi, J. S., Ip, C. W., Jamieson, S., Jamora, R. D. G., Jech, R., Jeon, B. S., Kaminska, A., Karpova, M., Khasanova, D., Kim, J. M., Kim, J. W., Kok, C. Y., Korenko, A., Korv, J., Koussa, S., Kovacs, T., Kreisler, A., Krystkowiak, P., Kumthornthip, W., Lin, C. H., Lundin, F., Lus, G., Magalhaes, M., Masmoudi, A. N., Mercelis, R., Misbahuddin, A., Moebius, C., Mohammadi, B., Nazem, B., Ng, K., Nurlu, G., Nyberg, J., Nyholm, D., Ochudlo, S., Otruba, P., Pfister, R., Pirtosek, Z., Pokhabov, D., Quinones Aguilar, S., Quinones Canales, G., Raghev, S., Rickmann, H., Romano, M., Rosales, R. L., Rubanovits, I., Santilli, V., Schoels, L., Simonetta-Moreau, M., Simu, M. A., Sohn, Y. H., Soulayrol, S., Supe, I., Svetel, M., Sycha, T., Tan, E. K., Timerbaeva, S., Tokcaer, A. B., Trosch, R., Tugnoli, V., Tumas, V., van der Linden, C., Vetra, A., Vial, C., Vidry, E., Williams, D., Wimalaratna, S., and Yiannikas, C.
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Male ,Neurology ,SATISFACTION ,International Cooperation ,Cohort Studies ,0302 clinical medicine ,QUALITY-OF-LIFE ,Botulinum toxin ,Observational study ,Tremor ,Epidemiology ,030212 general & internal medicine ,Cervical dystonia ,Botulinum Toxins, Type A ,Torticollis ,Neuroradiology ,BLEPHAROSPASM ,education.field_of_study ,Original Communication ,INTEREST IN CD2 study group ,Middle Aged ,Treatment Outcome ,Neuromuscular Agents ,Female ,Life Sciences & Biomedicine ,medicine.drug ,Adult ,medicine.medical_specialty ,Population ,Clinical Neurology ,DIAGNOSIS ,03 medical and health sciences ,Patient satisfaction ,Neurology (clinical) ,Internal medicine ,medicine ,Humans ,education ,Aged ,Science & Technology ,Neurology & Neurosurgery ,Electromyography ,GUIDANCE ,business.industry ,1103 Clinical Sciences ,medicine.disease ,NEUROTOXIN ,REGISTRY ,UPDATE ,Neurosciences & Neurology ,1109 Neurosciences ,business ,030217 neurology & neurosurgery - Abstract
Background Longitudinal cohort studies provide important information about the clinical effectiveness of an intervention in the routine clinical setting, and are an opportunity to understand how a population presents for treatment and is managed. Methods INTEREST IN CD2 (NCT01753349) is a prospective, international, 3-year, longitudinal, observational study following the course of adult idiopathic cervical dystonia (CD) treated with botulinum neurotoxin type A (BoNT-A). The primary objective is to document long-term patient satisfaction with BoNT-A treatment. Here we report baseline data. Results This analysis includes 1036 subjects (67.4% of subjects were female; mean age was 54.7 years old; mean TWSTRS Total score was 31.7). BoNT-A injections were usually given in line with BoNT-A prescribing information. The most commonly injected muscles were splenius capitis (87.3%), sternocleidomastoid (82.6%), trapezius (64.3%), levator scapulae (40.9%) and semispinalis capitis (26.9%); 35.5% of subjects were injected using a guidance technique. Most subjects (87.8%) had been previously treated with BoNT-A (median interval between last pre-study injection and study baseline was 4 months); of these 84.8% reported satisfaction with BoNT-A treatment at peak effect during their previous treatment cycle and 51.5% remained satisfied at the end of the treatment. Analyses by geographical region revealed heterogeneity in the clinical characteristics and BoNT-A injection practice of CD subjects presenting for routine treatment. Conclusions These baseline analyses provide sizeable data regarding the epidemiology and clinical presentation of CD, and demonstrate an international heterogeneity of clinical practice. Future longitudinal analyses of the full 3-year study will explore how these factors impact treatment satisfaction. Electronic supplementary material The online version of this article (10.1007/s00415-017-8698-2) contains supplementary material, which is available to authorized users.
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- 2017
5. Evaluation of VEGF A expression and microvascular density as prognostic factors in extrahepatic cholangiocarcinoma
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Möbius, C., Demuth, C., Aigner, T., Wiedmann, M., Wittekind, C., Mössner, J., Hauss, J., and Witzigmann, H.
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- 2007
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6. Histopathological outcome of 597 isolated soft tissue tumors suspected of soft tissue sarcoma: A single-center 12-year experience
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Leinung, S., Möbius, C., Udelnow, A., Hauss, J., and Würl, P.
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- 2007
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7. COX2 expression, angiogenesis, proliferation and survival in Barrett's cancer
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Möbius, C., Stein, H.J., Spieß, C., Becker, I., Feith, M., Theisen, J., Gais, P., Jütting, U., and Siewert, J.R.
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- 2005
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8. How satisfied are cervical dystonia patients after 3 years of botulinum toxin type A treatment? Results from a prospective, long-term observational study
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Colosimo, C, Charles, D, Misra, VP, Maisonobe, P, Om, S, Abdulnayef, A, Adatepe, NU, Leite, AMA, Badarny, S, Bajenaru, O, Bares, M, Bejjani, P, Bergmans, B, Bhidayasiri, R, Bozic, H, Costa, CFE, Carlstrom, C, Castelnovo, G, Chang, MH, Chang, YY, Chung, TM, Coletti-Moja, M, Delvaux, V, Dioszhegy, P, Dogu, O, Duzynski, W, Ehler, E, Sierra, EL, Fabbrini, G, Ferreira, J, Valadas, FA, Foresti, C, Girlanda, P, Goh, KJ, Velon, GA, Grill, S, Gurevitch, T, Hadidi, M, Hamimed, MA, Hamri, A, Harrower, T, Hassin, S, Hedera, P, Hernandez, JFJG, Franco, HJ, Ho, B, Ho, SL, Hughes, A, Ilic, T, Inshasi, JS, Ip, CW, Jamieson, S, Jamora, RDG, Jech, R, Jeon, BS, Kaminska, A, Karpova, M, Khasanova, D, Kim, JM, Kim, JW, Kok, CY, Korenko, A, Korv, J, Koussa, S, Kovacs, T, Kreisler, A, Krystkowiak, P, Kumthornthip, W, Lin, CH, Lundin, F, Lus, G, Magalhaes, M, Masmoudi, AN, Mercelis, R, Misbahuddin, A, Moebius, C, Mohammadi, B, Nazem, B, Ng, K, Nurlu, G, Nyberg, J, Nyholm, D, Ochudlo, S, Otruba, P, Pfister, R, Pirtosek, Z, Pokhabov, D, Aguilar, QS, Canales, QG, Raghev, S, Rickmann, H, Romano, M, Rosales, RL, Rubanovits, I, Santilli, V, Schoels, L, Simonetta-Moreau, M, Ma, S, Sohn, YH, Soulayrol, S, Supe, I, Svetel, M, Sycha, T, Tan, EK, Timerbaeva, S, Tokcaer, AB, Trosch, R, Tugnoli, V, Tumas, V, Van der Linden, C, Vetra, A, Vial, C, Vidry, E, Williams, D, Wimalaratna, S, and Yiannikas, C
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0301 basic medicine ,Male ,Pediatrics ,Neurology ,SATISFACTION ,Botulinum toxin ,Cervical dystonia ,Observational study ,Satisfaction ,Treatment ,0302 clinical medicine ,QUALITY-OF-LIFE ,Outcome Assessment, Health Care ,Prospective Studies ,Botulinum Toxins, Type A ,Torticollis ,Neuroradiology ,BLEPHAROSPASM ,INTEREST IN CD2 study group ,Middle Aged ,Neuromuscular Agents ,Patient Satisfaction ,SAFETY ,Female ,Life Sciences & Biomedicine ,medicine.drug ,Adult ,medicine.medical_specialty ,Adolescent ,MEDLINE ,Clinical Neurology ,Treatment results ,DIAGNOSIS ,03 medical and health sciences ,Young Adult ,medicine ,Humans ,Aged ,Science & Technology ,Neurology & Neurosurgery ,business.industry ,Correction ,1103 Clinical Sciences ,medicine.disease ,EFFICACY ,030104 developmental biology ,Neurology (clinical) ,Neurosciences & Neurology ,business ,FOLLOW-UP ,1109 Neurosciences ,030217 neurology & neurosurgery ,Botulinum toxin type - Abstract
Background Patients with cervical dystonia (CD) typically require regular injections of botulinum toxin to maintain symptomatic control. We aimed to document long-term patient satisfaction with CD symptom control in a large cohort of patients treated in routine practice. Methods This was a prospective, international, observational study (NCT01753349) following the course of adult CD treated with botulinum neurotoxin type A (BoNT-A) over 3 years. A comprehensive clinical assessment status was performed at each injection visit and subjects reported satisfaction in two ways: satisfaction with symptom control at peak effect and at the end of treatment cycle. Results Subject satisfaction remained relatively stable from the first to the last injection visit. At 3 years, 89.9% of subjects reported satisfaction with symptom control at peak effect and 55.6% reported satisfaction with symptom control at end of treatment cycle. By contrast, objective ratings of CD severity showed an overall reduction over 3 years. Mean ± SD Toronto Western Spasmodic Rating Scale (TWSTRS) Total scores (clinician assessed at end of treatment cycle) decreased from 31.59 ± 13.04 at baseline to 24.49 ± 12.43 at 3 years (mean ± SD reduction from baseline of − 6.97 ± 11.56 points). Tsui scale scores also showed gradual improvement; the percent of subjects with a tremor component score of 4 reduced from 12.4% at baseline to 8.1% at 3 years. Conclusions Despite objective clinical improvements over 3 years, subject satisfaction with symptom control remained relatively constant, indicating that factors other than symptom control also play a role in patient satisfaction.
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- 2019
9. REVERSE WILL ROGERS PHENOMENON in HILAR CHOLANGIOCARCINOMA: 13
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Moebius, C, Wiedmann, M, Caca, K, Uhlmann, D, Hauss, J, and Witzigmann, H
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- 2005
10. Preoperative chemotherapy unmasks underlying Barrett’s mucosa in patients with adenocarcinoma of the distal esophagus
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Theisen, J., Stein, H. J., Dittler, H. J., Feith, M., Moebius, C., Kauer, W. K. H., Werner, M., and Siewert, J. R.
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- 2002
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11. Neovaskularisation und VEGF Expression beim Barrett Karzinom
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Möbius, C., Stein, H.-J., Becker, I., Feith, M., Gais, P., Jütting, J., Siewert, J. R., Hartel, W., editor, and Siewert, J. R.
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- 2002
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12. The Geographical Distribution and Habits of Whales
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Möbius, C.
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- 1894
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13. The ‘angiogenic switch’ in the progression from Barrett's metaplasia to esophageal adenocarcinoma
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Möbius, C, Stein, H.J, Becker, I, Feith, M, Theisen, J, Gais, P, Jütting, U, and Siewert, J.R
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- 2003
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14. Involvement of obliquus capitis inferior muscle in dystonic head tremor
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Schramm, A., Huber, D., Möbius, C., Münchau, A., Kohl, Z., and Bäumer, T.
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- 2017
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15. Protective efficacy of phosphodiesterase-1 inhibition against alpha-synuclein toxicity revealed by compound screening in LUHMES cells
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Höllerhage, M., Moebius, C., Melms, J., Chiu, W.H., Goebel, J.N., Chakroun, T., Koeglsperger, T., Oertel, W.H., Rösler, T.W., Bickle, M., and Höglinger, G.U.
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nervous system - Abstract
α-synuclein-induced neurotoxicity is a core pathogenic event in neurodegenerative synucleinopathies such as Parkinson's disease, dementia with Lewy bodies, or multiple system atrophy. There is currently no disease-modifying therapy available for these diseases. We screened 1,600 FDA-approved drugs for their efficacy to protect LUHMES cells from degeneration induced by wild-type α-synuclein and identified dipyridamole, a non-selective phosphodiesterase inhibitor, as top hit. Systematic analysis of other phosphodiesterase inhibitors identified a specific phosphodiesterase 1 inhibitor as most potent to rescue from α-synuclein toxicity. Protection was mediated by an increase of cGMP and associated with the reduction of a specific α-synuclein oligomeric species. RNA interference experiments confirmed PDE1A and to a smaller extent PDE1C as molecular targets accounting for the protective efficacy. PDE1 inhibition also rescued dopaminergic neurons from wild-type α-synuclein induced degeneration in the substantia nigra of mice. In conclusion, this work identifies inhibition of PDE1A in particular as promising target for neuroprotective treatment of synucleinopathies.
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- 2017
16. E 26 - Karzinome des Magens und ösophagogastralen Übergangs
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Moehler, M., Galle, P.R., Möbius, C., and Jonas, S.
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- 2015
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17. E 25 - Benigne Magentumoren
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Secknus, R., Mössner, J., Möbius, C., and Jonas, S.
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- 2015
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18. P 157 Detection radius of EMG for fasciculations: combined ultrasonographic-electromyographic analysis
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Regensburger, M., Tenner, F., Möbius, C., and Schramm, A.
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- 2017
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19. Autorenverzeichnis
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Andresen, V., Angenendt, J., Anthoni, C., Appenrodt, B., Arbogast, M., Arco, G., Atta, J., Auer, M., Auernhammer, C., Autenrieth, I.B., Avenhaus, W., Bachem, R., Backmund, M., Bänsch, D., Ballauff, A., Baltzer, J., Barth, J., Batra, A., Bazarra-Castro, M.A., Beck, S., Becker, K., Becker, Karsten, Behr, J., Behrens, A., Belyaev, O., Bender-Götze, Ch., Bengel, J., Benz, M., Haunerschen, von, Berberich, J., Berger, M., Berner, R., Berr, F., S.C., Blank, N., Bleh, C., Blind, Eberhard, Blum, H.E., Bock, N., Bockhorn, M., Böhler, J., Böhm, M., Bokemeyer, D., Bönner, G., Bork, K., Born, G., Brandt, Thomas, Braun, J., Bruch, H.-P., Brümmendorf, T.H., Brüwer, M., Brunnberg, U., Buchfelder, M., Buchkremer, G., Büchler, M.W., Carl, H.-D., Castell, S., Daniels, C., Daum, S., Detter, C., Deuschl, G., Dieckmann, E., Diederich, S., Diehm, C., Diemer, T., Diener, H.C., Diepolder, H., Distler, J., Dörner, T., Prof. Dr., Domagk, D., Domschke, W., Dragu, A., Dralle, H., Dreyling, M., van, P., Dürk, T., Ebert, D., Ehlebracht-König, I., Elger, C.E., Ell, C., Ellinger, J., Emons, G., Engel, O., Enzensberger, W., Epple, H.-J., Erbel, R., Fassnacht, M., Feußner, Hubertus, Fichter, M., Fiegel, P., Filipas, D., Fisang, C., Fisch, M., Fischbach, W., Fischer, N., Fischer, M., Flamme, C.H., Fleckenstein, K., Floege, J., Fluhr, G., Fölsch, U.R., Forsting, M., Fottner, C., Frank, W., Frey, N., Freyberger, H., Friese, K., Frilling, A., habil, PD. Dr., Frommberger, U., Frühmorgen, P., Fuss, Johannes, Gätje, R., Galle, P.R., Geidel, S., Geiß, H.-Ch., Genth, Ekkehard, Gilsbach, J.M., Gingelmaier, A., Goebel, F.-D., Göhl, J., Gökbuget, N., Gold, R., Gonzalez-Carmona, M.A., Gossé, F., Grabitz, K., Greetfeld, M., Gries, F.A., Grosch-Wörner, I., Grüner, N., Grünke, M., Grüters-Kieslich, A., Gülberg, V., Haak, T., Häfner, R., Härter, M., Hagenacker, T., Hahn, S., Hahner, S., Haidl, G., Hammer, M., Hammersen, F., Handrick, W., Hanisch, F., Hansen, M.P., Hanke, Sara, Haschka, J., Hasslacher, C., Hauer, Th., Hauptmann, A., Heckmann, M., Heidbreder, E., Heim, U., Heindel, W., Heitmann, J., Hegenbart, U., Hermann, W., Herrmann, J.M., Herpertz-Dahlmann, B., Heßlinger, B., Heuß, D., Heußner, P., Hiller, E., Hirner, A., Hölscher, A.H., Hölzen, J., Hörl<ce:sup loc='post">†</ce:sup>, W.H., Hörle, S., Hof, H., Hofmann, W.-K., Hohenberger, W., Hohenfellner, U., Holler, E., Holtmann, G., Honegger, J., Hopf, H.C., Horch, R.E., Hornke, I., Hornung, T., Huber, R.M., Hueber, A., Hübner, J., Hummel, R., Irmscher, S., Janßen, O.E., Jelinek, T., Jendrissek, K.A., Jonas, S., Jost, E., Jung, H.H., Kahaly, G.J., Kalden, J.R., Kalff, J., Kapellen, T., Karaus, M., Kastrup, O., Katsoulis, S., Katus, H., Kaudel, C.P., Kaulitz, R., Keck, C., Keller, F., Kellnar, S., Kiehne, K., Kiess, W., Kindermann, M., Kirschbaum, A., Klein, M., Kleindienst, A., Kneitz, C., von Kodolitsch, Y., Köhler, D., Kessler, H.P., Köhler, G., Köhler, H., Köhler, L., Köhler, M., Köhnke, M., Königs, C., Köninger, J., Könsgen-Mustea, D., Köster, R., Kötter, I., Kohne, E., Kolb, H.-J., Koletzko, S., Kollmar, R., Konstantinidis, S., Koop, K., Kopp, H.G., Koschinsky, T., Kramer, H.J., Krauss, J., Kreis, M.E., Kremer, B., Kroemer, H.K., Kröner-Herwig, B., Kroll, P., Külz, A.K., Kuhl, H., Kuipers, J.G., Laaser, M., Lamla, U., Lammert, F., Langer, M., Laß, M., Laukötter, M., Layer, P., Leffler, M., Lehnert, H., Lehrke, M., Lembcke, B., Lerch, M.M., Liebe, S., Lieber, A., Limmroth, V., Lochs, H., Loddenkemper, R., Löhr, J.-M., Löscher, T., Loh, A., Lorenz, H.-M., Lorenz, J., Lügering, N., Luster, M., Lux, G., Luzar, O., Maercker, A., Magdorf, K., Mallmann, P., Marth, T., May, K., Mayerle, J., Meinertz, T., Melichar, V., Merle, U., Meyer, H.J., Meyer, Th., Meyer-Lehnert, H., Meyer-Marcotty, A., Michels, H., Möbius, C., Möddel, G., Möhler, M., Mönnikes, H., Mössner, J., Mohaupt, M.G., Müller, S.C., Müller, S.A., Müller-Lissner, S., Müller-Quernheim, J., Muntau, A., Musholt, T.J., Nacimiento, W., Nattermann, J., Nelles, G., Neubrand, M., Neuhäuser, C., Neuhaus, P., Neumann, P.-A., Neundörfer, B., Nicolai, T., Niebling, W.-B., Niehues, T., Nilius, G., Nolde, J., Noth, J., Olschewski, H., Ostermeyer, J., Ott, C., Pahernik, S., Palmes, D., Pankratius, U., Parhofer, K., Paschke, R., Passlick, B., Pech, O., Pelster, F.W., Petersen, E.E., Petri, E., Pfaffenbach, B., Pfeifer, M., Pfeiffer, T., Pfister, H.W., Diplom-Gesundheitswirt, Pickel, J., Pilatz, A., Pirlich, M., Polykandriotis, E., Pontz, B., Possinger, K., Pohl-Koppe, A., Pohle, T., Prange, H., Prasse, A., Pruß, A., Rädle, J., Raile, K., Randerath, W., Rascher, W., Rauch, B., Raue, F., Raziorruh, B., Rech, J., Regierer, A.C., Reichel, C., Reindl, C., Reinhardt, D., Reißfelder, C., Rendl, J., Reuss-Borst, M., Rieckmann, P., Riedner, C., Rietschel, E., Rijcken, E., Rister, M., Rödder, K., Rogenhofer, S., Roos, F.C., Roos, R., Rosskopf, D., Rudnik-Schöneborn, S., Rudofsky<ce:sup loc='post">†</ce:sup>, G., Ruhnke, M., Ruß, M., Rust, C.F., Saborowski, F., Sailer, M., Salakdeh, M. Sedigh, Samtleben, Walter, Sandmann, W., Sauerbruch, T., Schaal, K.P., Schackert, G., Schäfer-Graf, U., Schäfers, M., Schalhorn, A., Schepp, W., Schetelig, J., Schifferdecker, M., Schipper, J., Schießl, A., Schlegel, U., Schliep, S., Schmid, A., Schmid, P., Schmidt, F., Schmied, B., Schmiegel, W., Schneider, A., Schneider, T., Schneider-Gold, C., Schnürch, H.-G., Schölmerich, J., Schönermarck, U., Schönhofer, B., Schönland, S., Scholz, H., Schopohl, J., Schott, G., Schrader, J., Schraml, A., Schrezenmeier, H., Schuchert, A., Schüßler, G., Schulze-Koops, H., Schuppan, D., Schuster, V., Schwab, S., Schwandner, O., Schwarz, C.H.M., Schwarz, T.F., Schweppe, K.W., Secknus, R., Segerer, S.E., Senninger, N., Serve, H., Seybold, U., Sezer, O., Siegmund, B., Siegmund, W., Siemon, G., Simmen, B.R., Simonetti, G., Sommer, C., Spengler, U., Sprott, H., Stabenow-Lohbauer, U., Stahl, M., Stalla, G., Stallmach, A., Stammschulte, T., Stebler, R., Stein, R., Steven, D., Sticherling, M., Stöhr, M., Strauch, U., Strauss, A., Strauß, H.-G., Stremmel, C., Stremmel, W., Strupp, M., Stüber, E., Stürz, H., Sure, U., Swoboda, B., Taube, C., Thiel, K., Thomssen, C., Thurau, K., Thöne, J., Thüroff, J., Tomiak, C., Toyka, K.V., Tröger, H., Trüeb, R.M., Tryba, M., Uhl, W., Ullerich, H., Unger, L., Vallböhmer, D., van Calker, D., Vloet, T., Voderholzer, U., Völkl, Thomas M.K., Vogel, T., Vogt, P., Wagenlehner, F.E.M., Wagner, A., Wagner, U., Wahn, V., Wallesch, C.W., Watzka, F., Weber, K., Weber, L., Weber, M.M., Wehrmann, T., Weidner, W., Weinke, T., Weiß, M., Weis-Müller, B.T., Weller, Michael, Wenz, F., Werdan, K., Wettstein, M., Wick, M., Wiegratz, I., Willems, S., Wilke, H., Wintergerst, U., Wirth, M., Wolkersdörfer, G.W., Wüster, C., Zabel, F., Zeidler, H., Zeitz, M., Zerres, K., Ziemer, G., Zierz, S., Zimmermann, T., and Zwerina, J.
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- 2015
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20. Duodenalperforation nach abdominalem Trauma
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Schneider, R., primary, Moebius, C., additional, Thelen, A., additional, and Jonas, S., additional
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- 2009
- Full Text
- View/download PDF
21. Bendamustine - A New Approach to the Treatment of Advanced Hepatocellular Carcinoma?
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Wiedmann, M., primary, Zeh, J., additional, Schoppmeyer, K., additional, Moebius, C., additional, Niederhagen, M., additional, Wittekind, C., additional, Mossner, J., additional, and Caca, K., additional
- Published
- 2008
- Full Text
- View/download PDF
22. Treatment of pancreatic cancer with two novel histone deacetylase inhibitors NVP-LAQ824 and NVP-LBH589
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Wiedmann, M., primary, Haefner, M., additional, Niederhagen, M., additional, Schoppmeyer, K., additional, Wittekind, C., additional, Moebius, C., additional, Mossner, J., additional, and Caca, K., additional
- Published
- 2007
- Full Text
- View/download PDF
23. Evidence for a relationship between recovery from anaesthesia, modified state of consciousness and striatal voltammetric levels of ascorbic acid
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Crespi, F., Möbius, C., and Keane, P.
- Published
- 1992
- Full Text
- View/download PDF
24. VEGF expression and neovascularisation in Barrett's carcinoma and its premalignant lesions
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Moebius, C., Becker, I., Feith, M., Siewert, J.R., and Stein, H.J.
- Published
- 2001
- Full Text
- View/download PDF
25. Short-range differential pulse voltammetry for fast, selective analysis of basal levels of cerebral compounds in vivo
- Author
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Crespi, F., Moebius, C., and Neudeck, A.
- Published
- 1993
- Full Text
- View/download PDF
26. Statistical integration of multi-omics and drug screening data from cell lines.
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El Bouhaddani S, Höllerhage M, Uh HW, Moebius C, Bickle M, Höglinger G, and Houwing-Duistermaat J
- Subjects
- Humans, Multiomics, Drug Evaluation, Preclinical, Proteomics methods, Computational Biology methods, Synucleinopathies
- Abstract
Data integration methods are used to obtain a unified summary of multiple datasets. For multi-modal data, we propose a computational workflow to jointly analyze datasets from cell lines. The workflow comprises a novel probabilistic data integration method, named POPLS-DA, for multi-omics data. The workflow is motivated by a study on synucleinopathies where transcriptomics, proteomics, and drug screening data are measured in affected LUHMES cell lines and controls. The aim is to highlight potentially druggable pathways and genes involved in synucleinopathies. First, POPLS-DA is used to prioritize genes and proteins that best distinguish cases and controls. For these genes, an integrated interaction network is constructed where the drug screen data is incorporated to highlight druggable genes and pathways in the network. Finally, functional enrichment analyses are performed to identify clusters of synaptic and lysosome-related genes and proteins targeted by the protective drugs. POPLS-DA is compared to other single- and multi-omics approaches. We found that HSPA5, a member of the heat shock protein 70 family, was one of the most targeted genes by the validated drugs, in particular by AT1-blockers. HSPA5 and AT1-blockers have been previously linked to α-synuclein pathology and Parkinson's disease, showing the relevance of our findings. Our computational workflow identified new directions for therapeutic targets for synucleinopathies. POPLS-DA provided a larger interpretable gene set than other single- and multi-omic approaches. An implementation based on R and markdown is freely available online., Competing Interests: The authors have declared that no competing interests exist., (Copyright: © 2024 el Bouhaddani et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.)
- Published
- 2024
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- View/download PDF
27. Loss of a gluconeogenic muscle enzyme contributed to adaptive metabolic traits in hummingbirds.
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Osipova E, Barsacchi R, Brown T, Sadanandan K, Gaede AH, Monte A, Jarrells J, Moebius C, Pippel M, Altshuler DL, Winkler S, Bickle M, Baldwin MW, and Hiller M
- Subjects
- Animals, Energy Metabolism genetics, Birds genetics, Birds metabolism, Flight, Animal physiology, Gluconeogenesis genetics, Adaptation, Physiological genetics, Fructose-Bisphosphatase genetics, Muscle, Skeletal enzymology
- Abstract
Hummingbirds possess distinct metabolic adaptations to fuel their energy-demanding hovering flight, but the underlying genomic changes are largely unknown. Here, we generated a chromosome-level genome assembly of the long-tailed hermit and screened for genes that have been specifically inactivated in the ancestral hummingbird lineage. We discovered that FBP2 (fructose-bisphosphatase 2), which encodes a gluconeogenic muscle enzyme, was lost during a time period when hovering flight evolved. We show that FBP2 knockdown in an avian muscle cell line up-regulates glycolysis and enhances mitochondrial respiration, coincident with an increased mitochondria number. Furthermore, genes involved in mitochondrial respiration and organization have up-regulated expression in hummingbird flight muscle. Together, these results suggest that FBP2 loss was likely a key step in the evolution of metabolic muscle adaptations required for true hovering flight.
- Published
- 2023
- Full Text
- View/download PDF
28. Separating Response of Tumor and non-Tumor Cells to Drug In Vitro by Quantifying a Mutation.
- Author
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Kleinpoppen M, Moebius C, Grupp K, Kluwe L, and Blessmann M
- Subjects
- Biomarkers, Tumor metabolism, Cell Line, Tumor, Cell Survival drug effects, Dose-Response Relationship, Drug, Feasibility Studies, Humans, Melanoma enzymology, Melanoma genetics, Melanoma pathology, Polymerase Chain Reaction, Proto-Oncogene Proteins B-raf metabolism, Skin Neoplasms enzymology, Skin Neoplasms genetics, Skin Neoplasms pathology, Antineoplastic Agents pharmacology, Biomarkers, Tumor antagonists & inhibitors, Biomarkers, Tumor genetics, DNA Mutational Analysis methods, Melanoma drug therapy, Mutation, Proto-Oncogene Proteins B-raf antagonists & inhibitors, Proto-Oncogene Proteins B-raf genetics, Skin Neoplasms drug therapy, Vemurafenib pharmacology
- Abstract
Background/aim: Conventional in vitro assays measure the effect of drugs on total cells, while separating the effect to those on tumor and non-tumor cells is important for assessing drug specificity. Our aim was to evaluate the feasibility of separating the efficacy of vemurafenib on tumor and non-tumor cells in a mixed culture., Materials and Methods: Melanoma A2058 cells and CCD18Co non-tumor cells were mixed and treated with vemurafenib. DNA was subjected to digital PCR to determine the ratio of the mutant 1799A to the wild-type 1799T alleles and viabilities of total cells were subsequently calculated as percentages of tumor and non-tumor cells., Results: The set-up proportion of tumor cells correlated well with the calculated one. The calculated viability of tumor cells decreased with increasing doses of vemurafenib while that of the non-tumor cells remained rather constant. Variability of digital PCR data was high., Conclusion: Using the BRAF mutation 1799T>A to separate the response of tumor and non-tumor cells to a drug, such as vemurafenib, is feasible, supporting a foundation for a genetic in vitro tool for testing drug efficacy and specificity., (Copyright© 2019, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.)
- Published
- 2019
- Full Text
- View/download PDF
29. Prevalence and clinical significance of RBM3 immunostaining in non-small cell lung cancers.
- Author
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Melling N, Bachmann K, Hofmann B, El Gammal AT, Reeh M, Mann O, Moebius C, Blessmann M, Izbicki JR, and Grupp K
- Subjects
- Adult, Aged, Aged, 80 and over, Biomarkers, Tumor biosynthesis, Carcinoma, Non-Small-Cell Lung pathology, Female, Humans, Immunohistochemistry, Lung Neoplasms pathology, Male, Middle Aged, Neoplasm Staging, Prevalence, Tissue Array Analysis, Carcinoma, Non-Small-Cell Lung metabolism, Lung Neoplasms metabolism, RNA-Binding Proteins biosynthesis
- Abstract
Introduction: Aberrant expression of RNA-binding motif protein 3 (RBM3) has been suggested as a prognostic biomarker in several malignancies., Materials and Methods: This study was performed to analyse the prevalence and clinical significance of RBM3 immunostaining in non-small cell lung cancers (NSCLCs). Therefore, we took advantage of our tissue microarray (TMA) containing more than 600 NSCLC specimens., Results: While nuclear RBM3 staining was always high in normal lung tissue, high RBM3 staining was only seen in 77.1% of 467 interpretable non-metastatic NSCLCs. Reduced RBM3 staining was significantly associated with advanced pathological tumor stage (pT) in NSCLCs (p = 0.0031). Subset analysis revealed that the association between reduced RBM3 staining and advanced pT stage was largely driven by the histological subgroup of lung adenocarcinoma (LUACs) (p = 0.0036). In addition, reduced RBM3 expression predicted shortened survival in LUAC patients (p = 0.0225)., Conclusions: In summary, our study shows that loss of RBM3 expression predicts worse clinical outcome in LUAC patients.
- Published
- 2019
- Full Text
- View/download PDF
30. Phenotype loss is associated with widespread divergence of the gene regulatory landscape in evolution.
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Roscito JG, Sameith K, Parra G, Langer BE, Petzold A, Moebius C, Bickle M, Rodrigues MT, and Hiller M
- Subjects
- Animals, Binding Sites, Conserved Sequence genetics, DNA, Intergenic genetics, Extremities embryology, Eye pathology, Genome, Lizards genetics, Mammals genetics, Molecular Sequence Annotation, Phenotype, Sequence Analysis, DNA, Snakes genetics, Transcription Factors metabolism, Biological Evolution, Gene Expression Regulation, Gene Regulatory Networks, Genetic Variation
- Abstract
Detecting the genomic changes underlying phenotypic changes between species is a main goal of evolutionary biology and genomics. Evolutionary theory predicts that changes in cis-regulatory elements are important for morphological changes. We combined genome sequencing, functional genomics and genome-wide comparative analyses to investigate regulatory elements in lineages that lost morphological traits. We first show that limb loss in snakes is associated with widespread divergence of limb regulatory elements. We next show that eye degeneration in subterranean mammals is associated with widespread divergence of eye regulatory elements. In both cases, sequence divergence results in an extensive loss of transcription factor binding sites. Importantly, diverged regulatory elements are associated with genes required for normal limb patterning or normal eye development and function, suggesting that regulatory divergence contributed to the loss of these phenotypes. Together, our results show that genome-wide decay of the phenotype-specific cis-regulatory landscape is a hallmark of lost morphological traits.
- Published
- 2018
- Full Text
- View/download PDF
31. Isogenic FUS-eGFP iPSC Reporter Lines Enable Quantification of FUS Stress Granule Pathology that Is Rescued by Drugs Inducing Autophagy.
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Marrone L, Poser I, Casci I, Japtok J, Reinhardt P, Janosch A, Andree C, Lee HO, Moebius C, Koerner E, Reinhardt L, Cicardi ME, Hackmann K, Klink B, Poletti A, Alberti S, Bickle M, Hermann A, Pandey UB, Hyman AA, and Sterneckert JL
- Subjects
- Amyotrophic Lateral Sclerosis metabolism, Amyotrophic Lateral Sclerosis pathology, Animals, Antidepressive Agents pharmacology, Antipyretics pharmacology, Autophagy genetics, CRISPR-Cas Systems, Drosophila, Drug Evaluation, Preclinical, Green Fluorescent Proteins genetics, Humans, Motor Neurons metabolism, Motor Neurons pathology, Mutation, Phosphatidylinositol 3-Kinases genetics, Proto-Oncogene Proteins c-akt genetics, Signal Transduction drug effects, TOR Serine-Threonine Kinases genetics, Amyotrophic Lateral Sclerosis genetics, Drosophila Proteins genetics, Heterogeneous-Nuclear Ribonucleoprotein Group F-H genetics, Induced Pluripotent Stem Cells metabolism, RNA-Binding Protein FUS genetics
- Abstract
Perturbations in stress granule (SG) dynamics may be at the core of amyotrophic lateral sclerosis (ALS). Since SGs are membraneless compartments, modeling their dynamics in human motor neurons has been challenging, thus hindering the identification of effective therapeutics. Here, we report the generation of isogenic induced pluripotent stem cells carrying wild-type and P525L FUS-eGFP. We demonstrate that FUS-eGFP is recruited into SGs and that P525L profoundly alters their dynamics. With a screening campaign, we demonstrate that PI3K/AKT/mTOR pathway inhibition increases autophagy and ameliorates SG phenotypes linked to P525L FUS by reducing FUS-eGFP recruitment into SGs. Using a Drosophila model of FUS-ALS, we corroborate that induction of autophagy significantly increases survival. Finally, by screening clinically approved drugs for their ability to ameliorate FUS SG phenotypes, we identify a number of brain-penetrant anti-depressants and anti-psychotics that also induce autophagy. These drugs could be repurposed as potential ALS treatments., (Copyright © 2018 The Authors. Published by Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
32. High-level βIII-tubulin overexpression occurs in most head and neck cancers but is unrelated to clinical outcome.
- Author
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Nienstedt JC, Gröbe A, Clauditz T, Simon R, Muenscher A, Knecht R, Sauter G, Moebius C, Blessmann M, Heiland M, and Pflug C
- Subjects
- Carcinoma, Squamous Cell chemistry, Female, Head and Neck Neoplasms chemistry, Humans, Immunohistochemistry, Male, Prognosis, Squamous Cell Carcinoma of Head and Neck, Tubulin analysis, Carcinoma, Squamous Cell genetics, Gene Expression Regulation, Neoplastic, Head and Neck Neoplasms genetics, Tubulin genetics
- Abstract
Background: βIII-tubulin (TUBB3) is an isotype of microtubules, which are involved in crucial cellular roles including maintenance of cell shape, intracellular transport, and mitosis. Overexpression of TUBB3 was found to be associated with poor prognosis and resistance to tubulin-binding drugs and in several solid tumors including head and neck squamous cell carcinomas (HNSCC). Considering the potential high importance of a prognostic biomarker in these cancers, this study aimed to investigate the clinical relevance of immunohistochemical TUBB3 expression in HNSCC., Methods: Tissue microarray (TMA) sections containing samples from 667 cancers of oral cavity, oro- and hypopharynx, and larynx for which follow-up data were available were analyzed for TUBB3 expression by immunohistochemistry., Results: Over 90% of our analyzed cancers showed unequivocal cytoplasmic TUBB3 expression. Staining was considered weak in 69 (15.5%), moderate in 149 (33.5%), and strong in 188 (42.2%) of cancers. The frequent TUBB3 overexpression showed no significant correlation with pathological grading, tumor stage, nodal status, or surgical margin and had no impact on patient outcomes., Conclusion: Despite lacking prognostic utility in HNSCC, the remarkable high prevalence of TUBB3 expression in HNSCC emphasizes its putative relevance as a target for future drugs targeting TUBB3., (© 2017 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.)
- Published
- 2017
- Full Text
- View/download PDF
33. Protective efficacy of phosphodiesterase-1 inhibition against alpha-synuclein toxicity revealed by compound screening in LUHMES cells.
- Author
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Höllerhage M, Moebius C, Melms J, Chiu WH, Goebel JN, Chakroun T, Koeglsperger T, Oertel WH, Rösler TW, Bickle M, and Höglinger GU
- Subjects
- Animals, Cell Line, Dipyridamole pharmacology, Drug Evaluation, Preclinical, High-Throughput Screening Assays, Humans, Mice, Neurons drug effects, Neurons metabolism, Neuroprotective Agents pharmacology, Protein Aggregation, Pathological drug therapy, Vinca Alkaloids pharmacology, alpha-Synuclein antagonists & inhibitors, Drug Discovery, Enzyme Inhibitors pharmacology, Phosphodiesterase I antagonists & inhibitors, Protein Aggregation, Pathological metabolism, alpha-Synuclein metabolism
- Abstract
α-synuclein-induced neurotoxicity is a core pathogenic event in neurodegenerative synucleinopathies such as Parkinson's disease, dementia with Lewy bodies, or multiple system atrophy. There is currently no disease-modifying therapy available for these diseases. We screened 1,600 FDA-approved drugs for their efficacy to protect LUHMES cells from degeneration induced by wild-type α-synuclein and identified dipyridamole, a non-selective phosphodiesterase inhibitor, as top hit. Systematic analysis of other phosphodiesterase inhibitors identified a specific phosphodiesterase 1 inhibitor as most potent to rescue from α-synuclein toxicity. Protection was mediated by an increase of cGMP and associated with the reduction of a specific α-synuclein oligomeric species. RNA interference experiments confirmed PDE1A and to a smaller extent PDE1C as molecular targets accounting for the protective efficacy. PDE1 inhibition also rescued dopaminergic neurons from wild-type α-synuclein induced degeneration in the substantia nigra of mice. In conclusion, this work identifies inhibition of PDE1A in particular as promising target for neuroprotective treatment of synucleinopathies.
- Published
- 2017
- Full Text
- View/download PDF
34. CD151 expression is frequent but unrelated to clinical outcome in head and neck cancer.
- Author
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Nienstedt JC, Gröbe A, Lebok P, Büscheck F, Clauditz T, Simon R, Heumann A, Sauter G, Moebius C, Münscher A, Knecht R, Blessmann M, Heiland M, and Pflug C
- Subjects
- Biomarkers, Tumor metabolism, Female, Head and Neck Neoplasms pathology, Humans, Immunohistochemistry, Male, Neoplasm Grading, Neoplasm Staging, Prognosis, Tissue Array Analysis, Head and Neck Neoplasms metabolism, Tetraspanin 24 metabolism
- Abstract
Objectives: CD151 is a plasma membrane protein belonging to the tetraspanin family. CD151 represents a putative therapeutic target and has been suggested as a prognostic marker in several cancer types. The present study aims to investigate the prognostic relevance of immunohistochemical CD151 expression in head and neck squamous cell carcinoma (HNSCC)., Materials and Methods: Tissue microarray (TMA) sections containing samples from 667 cancers of oral cavity, oro- and hypopharynx and larynx, for which follow-up data were available, were analyzed for CD151 expression by immunohistochemistry., Results: Membranous CD151 immunostaining was recorded in 269 (60.3 %) of 446 analyzable cases. Staining was considered weak in 129 (28.9 %), moderate in 98 (22.0 %), and strong in 42 (9.4 %) of cancers. CD151 expression was unrelated to histological grade, tumor stage, nodal status, or surgical margin. There was a tendency towards a somewhat lower prevalence of CD151 expression in tumors of the oral cavity (52.9 % positive) as compared to cancers of the oro-hypopharynx (62.1 %) and larynx (63.3 %; p = 0.0100). CD151 expression had no impact on patient survival., Clinical Relevance: In summary, immunohistochemical analysis of CD151 lacks prognostic utility in HNSCC. The high prevalence of CD151 expression in HNSCC emphasizes its putative relevance as a therapeutic target for further development of anti-CD151 drugs.
- Published
- 2017
- Full Text
- View/download PDF
35. Organotypic slice cultures of human gastric and esophagogastric junction cancer.
- Author
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Koerfer J, Kallendrusch S, Merz F, Wittekind C, Kubick C, Kassahun WT, Schumacher G, Moebius C, Gaßler N, Schopow N, Geister D, Wiechmann V, Weimann A, Eckmann C, Aigner A, Bechmann I, and Lordick F
- Subjects
- Antineoplastic Combined Chemotherapy Protocols therapeutic use, Biopsy, Combined Modality Therapy, Drug Resistance, Neoplasm, Esophageal Neoplasms drug therapy, Esophageal Neoplasms surgery, Humans, Organ Culture Techniques, Precision Medicine methods, Stomach Neoplasms drug therapy, Stomach Neoplasms surgery, Tissue Culture Techniques, Esophageal Neoplasms pathology, Esophagogastric Junction pathology, Stomach Neoplasms pathology
- Abstract
Gastric and esophagogastric junction cancers are heterogeneous and aggressive tumors with an unpredictable response to cytotoxic treatment. New methods allowing for the analysis of drug resistance are needed. Here, we describe a novel technique by which human tumor specimens can be cultured ex vivo, preserving parts of the natural cancer microenvironment. Using a tissue chopper, fresh surgical tissue samples were cut in 400 μm slices and cultivated in 6-well plates for up to 6 days. The slices were processed for routine histopathology and immunohistochemistry. Cytokeratin stains (CK8, AE1/3) were applied for determining tumor cellularity, Ki-67 for proliferation, and cleaved caspase-3 staining for apoptosis. The slices were analyzed under naive conditions and following 2-4 days in vitro exposure to 5-FU and cisplatin. The slice culture technology allowed for a good preservation of tissue morphology and tumor cell integrity during the culture period. After chemotherapy exposure, a loss of tumor cellularity and an increase in apoptosis were observed. Drug sensitivity of the tumors could be assessed. Organotypic slice cultures of gastric and esophagogastric junction cancers were successfully established. Cytotoxic drug effects could be monitored. They may be used to examine mechanisms of drug resistance in human tissue and may provide a unique and powerful ex vivo platform for the prediction of treatment response., (© 2016 The Authors. Cancer Medicine published by John Wiley & Sons Ltd.)
- Published
- 2016
- Full Text
- View/download PDF
36. A conserved RhoGAP limits M phase contractility and coordinates with microtubule asters to confine RhoA during cytokinesis.
- Author
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Zanin E, Desai A, Poser I, Toyoda Y, Andree C, Moebius C, Bickle M, Conradt B, Piekny A, and Oegema K
- Subjects
- Animals, Caenorhabditis elegans genetics, Caenorhabditis elegans growth & development, Cell Division genetics, Embryo, Nonmammalian cytology, Embryo, Nonmammalian metabolism, Gene Expression Regulation, Developmental, Microtubules genetics, Muscle Contraction genetics, rhoA GTP-Binding Protein metabolism, Cytokinesis genetics, GTPase-Activating Proteins genetics, Mitosis genetics, rhoA GTP-Binding Protein genetics
- Abstract
During animal cell cytokinesis, the spindle directs contractile ring assembly by activating RhoA in a narrow equatorial zone. Rapid GTPase activating protein (GAP)-mediated inactivation (RhoA flux) is proposed to limit RhoA zone dimensions. Testing the significance of RhoA flux has been hampered by the fact that the GAP targeting RhoA is not known. Here, we identify M phase GAP (MP-GAP) as the primary GAP targeting RhoA during mitosis and cytokinesis. MP-GAP inhibition caused excessive RhoA activation in M phase, leading to the uncontrolled formation of large cortical protrusions and late cytokinesis failure. RhoA zone width was broadened by attenuation of the centrosomal asters but was not affected by MP-GAP inhibition alone. Simultaneous aster attenuation and MP-GAP inhibition led to RhoA accumulation around the entire cell periphery. These results identify the major GAP restraining RhoA during cell division and delineate the relative contributions of RhoA flux and centrosomal asters in controlling RhoA zone dimensions., (Copyright © 2013 Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF
37. [Duodenal perforation after blunt abdominal trauma].
- Author
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Schneider R, Moebius C, Thelen A, and Jonas S
- Subjects
- Abdomen, Acute diagnosis, Abdomen, Acute surgery, Abdominal Abscess diagnosis, Abdominal Abscess surgery, Abdominal Injuries surgery, Anastomosis, Roux-en-Y, Diagnosis, Differential, Duodenum surgery, Female, Humans, Intestinal Perforation diagnosis, Middle Aged, Postoperative Complications diagnostic imaging, Postoperative Complications therapy, Retroperitoneal Space surgery, Tomography, X-Ray Computed, Wounds, Nonpenetrating surgery, Abdominal Injuries complications, Duodenum injuries, Intestinal Perforation surgery, Wounds, Nonpenetrating complications
- Abstract
Duodenal perforation after a blunt abdominal trauma is a rare emergency situation that can result in life-threatening complications. We report on a woman who had a perforation of the duodenum after a supposed mild blunt abdominal trauma. Unremarkable at the initial presentation, the patient presented with acute abdominal pain and a retroperitoneal abscess five days after the initial trauma. The duodenal repair was performed with a Roux-Y anastomosis. Difficulties in diagnosis are very common, but the early recognition of the rupture is essential. The contrast-enhanced CT scan is the gold standard for diagnosis. Surgical management depends on the severity of the trauma and must be chosen on an individual basis., (Georg Thieme Verlag Stuttgart-New York.)
- Published
- 2009
- Full Text
- View/download PDF
38. Experimental treatment of pancreatic cancer with two novel histone deacetylase inhibitors.
- Author
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Haefner M, Bluethner T, Niederhagen M, Moebius C, Wittekind C, Mossner J, Caca K, and Wiedmann M
- Subjects
- Animals, Antimetabolites, Antineoplastic pharmacology, Apoptosis drug effects, Cell Cycle drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Cyclin-Dependent Kinase Inhibitor p21 metabolism, Deoxycytidine analogs & derivatives, Deoxycytidine pharmacology, Dose-Response Relationship, Drug, Female, Histone Deacetylases metabolism, Humans, Immunoblotting, Immunohistochemistry, In Situ Nick-End Labeling, Indoles, Ki-67 Antigen metabolism, Mice, Mice, Nude, Pancreatic Neoplasms enzymology, Pancreatic Neoplasms pathology, Panobinostat, Time Factors, Gemcitabine, Antineoplastic Agents pharmacology, Enzyme Inhibitors pharmacology, Histone Deacetylase Inhibitors, Hydroxamic Acids pharmacology, Pancreatic Neoplasms drug therapy
- Abstract
Aim: To investigate in vitro and in vivo treatment with histone deacetylase inhibitors NVP-LAQ824 and NVP-LBH589 in pancreatic cancer., Methods: Cell-growth inhibition by NVP-LAQ824 and NVP-LBH589 was studied in vitro in 8 human pancreatic cancer cell lines using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. In addition, the anti-tumoral effect of NVP-LBH589 was studied in a chimeric mouse model. Anti-tumoral activity of the drugs was assessed by immunoblotting for p21(WAF-1), acH4, cell cycle analysis, TUNEL assay, and immunohistochemistry for MIB-1., Results: In vitro treatment with both compounds significantly suppressed the growth of all cancer cell lines and was associated with hyperacetylation of nucleosomal histone H4, increased expression of p21(WAF-1), cell cycle arrest at G2/M-checkpoint, and increased apoptosis. In vivo, NVP-LBH589 alone significantly reduced tumor mass and potentiated the efficacy of gemcitabine. Further analysis of the tumor specimens revealed slightly increased apoptosis and no significant reduction of cell proliferation., Conclusion: Our findings suggest that NVP-LBH589 and NVP-LAQ824 are active against human pancreatic cancer, although the precise mechanism of in vivo drug action is not yet completely understood. Therefore, further preclinical and clinical studies for the treatment of pancreatic cancer are recommended.
- Published
- 2008
- Full Text
- View/download PDF
39. Inhibition of histone deacetylase for the treatment of biliary tract cancer: a new effective pharmacological approach.
- Author
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Bluethner T, Niederhagen M, Caca K, Serr F, Witzigmann H, Moebius C, Mossner J, and Wiedmann M
- Subjects
- Animals, Apoptosis drug effects, Cell Cycle drug effects, Cell Line, Tumor, Cell Proliferation drug effects, Deoxycytidine analogs & derivatives, Deoxycytidine pharmacology, Deoxycytidine therapeutic use, Drug Therapy, Combination, Enzyme Inhibitors pharmacology, Humans, Hydroxamic Acids pharmacology, Hydroxamic Acids therapeutic use, Indoles, Mice, Mice, Nude, Panobinostat, Xenograft Model Antitumor Assays, Gemcitabine, Biliary Tract Neoplasms drug therapy, Enzyme Inhibitors therapeutic use, Histone Deacetylase Inhibitors
- Abstract
Aim: To investigate in vitro and in vivo therapeutic effects of histone deacetylase inhibitors NVP-LAQ824 and NVP-LBH589 on biliary tract cancer., Methods: Cell growth inhibition by NVP-LAQ824 and NVP-LBH589 was studied in vitro in 7 human biliary tract cancer cell lines by MTT assay. In addition, the anti-tumoral effect of NVP-LBH589 was studied in a chimeric mouse model. Anti-tumoral drug mechanism was assessed by immunoblotting for acH4 and p21( WAF-1/CIP-1), PARP assay, cell cycle analysis, TUNEL assay, and immunhistochemistry for MIB-1., Results: In vitro treatment with both compounds significantly suppressed the growth of all cancer cell lines [mean IC(50) (3 d) 0.11 and 0.05 micromol/L, respectively], and was associated with hyperacetylation of nucleosomal histone H4, increased expression of p21( WAF-1/CIP-1), induction of apoptosis (PARP cleavage), and cell cycle arrest at G2/M checkpoint. After 28 d, NVP-LBH589 significantly reduced tumor mass by 66% (bile duct cancer) and 87% (gallbladder cancer) in vivo in comparison to placebo, and potentiated the efficacy of gemcitabine. Further analysis of the tumor specimens revealed increased apoptosis by TUNEL assay and reduced cell proliferation (MIB-1)., Conclusion: Our findings suggest that NVP-LBH589 and NVP-LAQ824 are active against human biliary tract cancer in vitro. In addition, NVP-LBH589 demonstrated significant in vivo activity and potentiated the efficacy of gemcitabine. Therefore, further clinical evaluation of this new drug for the treatment of biliary tract cancer is recommended.
- Published
- 2007
- Full Text
- View/download PDF
40. History on a puppet stage.
- Author
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Moebius C
- Subjects
- Aged, California, Humans, Leisure Activities, Nursing Homes, Play and Playthings, Volunteers
- Published
- 1982
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