33 results on '"Mochi S"'
Search Results
2. Molecular epidemiology of methicillin-resistant Staphylococcus aureus from different population groups in Argentina
- Author
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Moreno Mochi, P., Vargas, J.M., Vivaldo, S., Bottiglieri, M., López, C., Mochi, S., Cobos, M., Castillo, M., del Campo, R., and Jure, M.A.
- Published
- 2020
- Full Text
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3. Immunological characterization of Toscana virus proteins
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Di Bonito, P., Nicoletti, L., Mochi, S., Accardi, L., Marchi, A., and Giorgi, C.
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- 1999
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4. A specific class of erythrocyte membrane microdomains is involved in Plasmodium falciparum invasion of the host cell
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Olivieri, A., Chaand, M., Fratini, F., Mangano, V., Pizzi, E., Celani, F., Mochi, S., Birago, C., Tirelli, V., Modiano, D., Duraisingh, M. T., and Ponzi, M.
- Published
- 2018
5. Role of erythrocyte membrane microdomains in Plasmodium falciparum infection
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Paone, S., Fratini, F., Pizzi, E., Tirelli, V., D'Alessandro, S., Parapini, S., Mangano, Valentina, Birago, C., Mochi, S., Modiano, D., Ponzi, M., and Olivieri, A.
- Published
- 2017
6. Role of erythrocyte membrane microdomains in Plasmodium falciparum infection
- Author
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Fratini, F., Pizzi, E., Tirelli, V., Paone, S., Grasso, F., Birago, C., Mochi, S., Ponzi, M., and Olivieri, A.
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plasmodium ,erythrocytes ,invasion - Published
- 2016
7. Molecular epidemiology of methicillin-resistant Staphylococcus aureusfrom different population groups in Argentina
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Moreno Mochi, P., Vargas, J.M., Vivaldo, S., Bottiglieri, M., López, C., Mochi, S., Cobos, M., Castillo, M., del Campo, R., and Jure, M.A.
- Abstract
•Molecular epidemiology of methicillin-resistant Staphylococcus aureus(MRSA) in a multiborder area of Argentina.•The prevalent staphylococcal cassette chromosome mectype was SCCmecIV.•The Cordobes/Chilean epidemic clone was not detected in the present MRSA collection.•ST5 was the most prevalent in colonisation, community and invasive settings.
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- 2020
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8. TT Virus Infection: Role of Interferons, Interleukin-28 and 29, Cytokines and Antiviral Proteins
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Garbuglia, A.R., primary, Grasso, F., additional, Donà, M.G., additional, Mochi, S., additional, Conti, P., additional, De Lutiis, M.A., additional, Giorgi, C., additional, and Iezzi, T., additional
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- 2007
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9. Completion of molecular characterization of Toscana phlebovirus genome: nucleotide sequence, coding strategy of M genomic segment and its amino acid sequence comparison to other phleboviruses
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Grò, M.C., primary, di Bonito, P., additional, Fortini, D., additional, Mochi, S., additional, and Giorgi, C., additional
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- 1997
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10. Organization of the M genomic segment of Toscana phlebovirus.
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Di Bonito, P, primary, Mochi, S, additional, Grò, M C, additional, Fortini, D, additional, and Giorgi, C, additional
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- 1997
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11. HPV and chlamydia trachomatis coinfection in women with pap smear abnormality: Baseline data of the HPV pathogen ISS study
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Mancini, F., Maria Fenicia Vescio, Mochi, S., Accardi, L., Di Bonito, P., and Ciervo, A.
12. Clinical and epidemiological correlates of antibody response to human papillomaviruses (HPVs) as measured by a novel ELISA based on denatured recombinant HPV16 late (L) and early (E) antigens
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Costa Silvano, Mariani Luciano, Branca Margherita, Donà Maria, Accardi Luisa, Mochi Stefania, Grasso Felicia, Di Bonito Paola, Giorgi Colomba, Agarossi Alberto, Ciotti Marco, and Syrjänen Kari
- Subjects
Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background At present, seroreactivity is not a valuable parameter for diagnosis of Human Papillomavirus (HPV) infection but, it is potentially valuable as marker of viral exposure in elucidating the natural history of this infection. More data are needed to asses the clinical relevance of serological response to HPV. Objectives The objective was to assess the clinical and epidemiological correlates of HPV-seroreactivity in a cohort of HIV-negative and HIV-positive women. Methods Seroreactivity of 96 women, evaluated in an ELISA test based on denatured HPV16 late (L) and early (E) antigens, was correlated with their clinical and epidemiological data previously collected for a multi-centre Italian study, HPV-PathogenISS study. Results No significant correlation was found between HPV DNA detection and seroreactivity. Women, current smokers showed significantly less seroreactivity to L antigens as compared with the non-smokers. HIV-positive women showed significantly less (66.7%) antibody response as compared with HIV-negative women (89.3%), with particularly impaired response to L antigens. Women, HIV-positive and current smokers, showed by far the lowest seroprevalence (33.3%) as compared to 75.9% among all other women (OR = 0.158; 95%CI 0.036–0.695, p = 0.014; Fisher's exact test). Importantly, this association did not loose its significance when controlled for confounding from age (continuous variable) in multivariate analysis or using Mantel-Haenszel test for age-groups. Conclusion It is tempting to speculate that HIV-positive current smokers comprise a special high-risk group, with highly impaired immunological response that could prevent eradication of persistent HPV infections and thus contribute to development of CIN3/CC.
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- 2008
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13. Serum antibody response to Human papillomavirus (HPV) infections detected by a novel ELISA technique based on denatured recombinant HPV16 L1, L2, E4, E6 and E7 proteins
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Mariani Luciano, Costa Silvano, Branca Margherita, Donà Maria, Accardi Luisa, Mochi Stefania, Grasso Felicia, Di Bonito Paola, Agarossi Alberto, Ciotti Marco, Syrjänen Kari, and Giorgi Colomba
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Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Background Human papillomaviruses (HPVs) are the primary etiological agents of cervical cancer and are also involved in the development of other tumours (skin, head and neck). Serological survey of the HPV infections is important to better elucidate their natural history and to disclose antigen determinants useful for vaccine development. At present, the analysis of the HPV-specific antibodies has not diagnostic value for the viral infections, and new approaches are needed to correlate the antibody response to the disease outcome. The aim of this study is to develop a novel ELISA, based on five denatured recombinant HPV16 proteins, to be used for detection HPV-specific antibodies. Methods The HPV16 L1, L2, E4, E6 and E7 genes were cloned in a prokaryotic expression vector and expressed as histidine-tagged proteins. These proteins, in a denatured form, were used in ELISA as coating antigens. Human sera were collected from women with abnormal PAP smear enrolled during an ongoing multicenter HPV-PathogenISS study in Italy, assessing the HPV-related pathogenetic mechanisms of progression of cervical cancer precursor lesions. Negative human sera were collected from patients affected by other infectious agents. All the HPV-positive sera were also subjected to an avidity test to assess the binding strength in the antigen-antibody complexes. Results Most of the sera showed a positive reactivity to the denatured HPV16 proteins: 82% of the sera from HPV16 infected women and 89% of the sera from women infected by other HPV genotypes recognised at least one of the HPV16 proteins. The percentages of samples showing reactivity to L1, L2 and E7 were similar, but only a few serum samples reacted to E6 and E4. Most sera bound the antigens with medium and high avidity index, suggesting specific antigen-antibody reactions. Conclusion This novel ELISA, based on multiple denatured HPV16 antigens, is able to detect antibodies in women infected by HPV16 and it is not genotype-specific, as it detects antibodies also in women infected by other genital HPVs. The assay is easy to perform and has low cost, making it suitable for monitoring the natural history of HPV infections as well as for detecting pre-existing HPV antibodies in women who receive VLP-based HPV vaccination.
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- 2006
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14. A Time Point Proteomic Analysis Reveals Protein Dynamics of Plasmodium Oocysts.
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Preira CMF, Pizzi E, Fratini F, Grasso F, Boccolini D, Mochi S, Favia G, Piselli E, Damiani C, Siden-Kiamos I, Ponzi M, and Currà C
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- Animals, Oocysts metabolism, Proteomics, Sporozoites metabolism, Protozoan Proteins metabolism, Anopheles, Plasmodium
- Abstract
The oocyst is a sporogonic stage of Plasmodium development that takes place in the mosquito midgut in about 2 weeks. The cyst is protected by a capsule of unknown composition, and little is known about oocyst biology. We carried out a proteomic analysis of oocyst samples isolated at early, mid, and late time points of development. Four biological replicates for each time point were analyzed, and almost 600 oocyst-specific candidates were identified. The analysis revealed that, in young oocysts, there is a strong activity of protein and DNA synthesis, whereas in mature oocysts, proteins involved in oocyst and sporozoite development, gliding motility, and invasion are mostly abundant. Among the proteins identified at early stages, 17 candidates are specific to young oocysts. Thirty-four candidates are common to oocyst and the merosome stages (sporozoite proteins excluded), sharing common features as replication and egress. Western blot and immunofluorescence analyses of selected candidates confirm the expression profile obtained by proteomic analysis., Competing Interests: Conflict of interest The authors declare no competing interests., (Copyright © 2024 The Authors. Published by Elsevier Inc. All rights reserved.)
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- 2024
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15. Emergence and clonal spread of KPC-2-producing clinical Klebsiella aerogenes isolates in a hospital from northwest Argentina.
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Vargas JM, Moreno Mochi MP, Nuñez JM, Mochi S, Cáceres M, Del Campo R, and Jure MA
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- Humans, beta-Lactamases genetics, Bacterial Proteins genetics, Klebsiella pneumoniae genetics, Argentina epidemiology, Microbial Sensitivity Tests, Anti-Bacterial Agents pharmacology, Hospitals, Enterobacter aerogenes, Klebsiella Infections epidemiology, Anti-Infective Agents, Cross Infection epidemiology
- Abstract
Introduction. Klebsiella aerogenes is a nosocomial pathogen associated with drug resistance and healthcare-associated infections. Gap Statement. K. aerogenes is associated with hospital-acquired infections with the ability to acquire mechanisms of resistance to reserve antimicrobials; its clinical behaviour has been poorly documented. Objective. We proposed to investigate an outbreak of carbapenem-resistant K. aerogenes in a hospital that persisted for 4 months. Methods. The primary aim was to evaluate the molecular characteristics and the clonal relationships among the isolates. We characterized isolates by polymerase chain reaction (PCR) and pulsed-field gel electrophoresis (PFGE). The information was integrated with clinical and epidemiological data. Results. Fourteen strains were disseminated in an intensive care unit and different wards at the hospital. The overall mortality was 42.8 %, and mortality attributed to infection was 21.4 %; strains showed high rates of resistance to most of the antimicrobials tested and carried bla
KPC-2 , blaSHV-2 and blaCTXM-15 genes. PFGE analysis indicated 2 PFGE groups; 12/14 isolates were associated with subgroup A and were likely to be primarily responsible for the first isolation and subsequent dissemination. The outbreak characteristics data showed prolonged hospitalization and previous use of antibiotics as potential risk factors. Conclusion. We consider that it is essential to perform phenotypic and genotypic identification of early genetic resistance mechanisms in K. aerogenes isolates, not only from infection sites but also from colonization, to prevent the spread of these multidrug-resistant (MDR) isolates.- Published
- 2023
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16. Identification and preliminary characterization of Plasmodium falciparum proteins secreted upon gamete formation.
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Grasso F, Fratini F, Albanese TG, Mochi S, Ciardo M, Pace T, Ponzi M, Pizzi E, and Olivieri A
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- Animals, Female, Germ Cells metabolism, Male, Mice, Plasmodium falciparum metabolism, Proteome metabolism, Protozoan Proteins genetics, Protozoan Proteins metabolism, Malaria parasitology, Malaria, Falciparum parasitology
- Abstract
Malaria long-term elimination depends on parasite transmission control. Plasmodium sexual stage maturation in the mosquito, including egress from the host erythrocyte, is one of the prime targets for transmission-blocking interventions. This work aims to identify candidate molecules potentially involved in gamete emergence from the host erythrocyte, as novel transmission blocking targets. We analyzed by quantitative mass spectrometry the proteins released/secreted by purified Plasmodium falciparum gametocytes upon induction of gametogenesis. The proteome obtained showed a good overlap (74%) with the one previously characterized in similar conditions from gametocytes of the rodent malaria parasite P. berghei. Four candidates were selected based on comparative analysis of their abundance values in released vs total gametocyte proteome. We also characterized the P. falciparum orthologue of the microgamete surface protein (MiGS), a marker of male gametocyte secretory vesicles in murine models of malaria. The findings of this study reveal that all the selected candidate proteins are expressed in both genders and localize to vesicle-like structures that respond to gametogenesis stimuli. This result, together with the fact that the selected proteins are released during gamete emergence in both Plasmodium species, makes them interesting candidates for future functional studies to investigate their potential role in the gametogenesis process., (© 2022. The Author(s).)
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- 2022
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17. Structural organization of erythrocyte membrane microdomains and their relation with malaria susceptibility.
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Olivieri A, Lee RS, Fratini F, Keutcha C, Chaand M, Mangano V, Celani F, Mochi S, Birago C, Paone S, Grasso F, Tirelli V, Falchi M, Shabani E, Bertoncini S, Sirima BS, Pizzi E, Modiano D, Duraisingh MT, and Ponzi M
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- Erythrocyte Membrane parasitology, Erythrocytes chemistry, Humans, Plasmodium falciparum physiology, Erythrocyte Membrane chemistry, Erythrocytes parasitology, Malaria parasitology, Malaria, Falciparum parasitology, Membrane Microdomains chemistry
- Abstract
Cholesterol-rich microdomains are membrane compartments characterized by specific lipid and protein composition. These dynamic assemblies are involved in several biological processes, including infection by intracellular pathogens. This work provides a comprehensive analysis of the composition of human erythrocyte membrane microdomains. Based on their floating properties, we also categorized the microdomain-associated proteins into clusters. Interestingly, erythrocyte microdomains include the vast majority of the proteins known to be involved in invasion by the malaria parasite Plasmodium falciparum. We show here that the Ecto-ADP-ribosyltransferase 4 (ART4) and Aquaporin 1 (AQP1), found within one specific cluster, containing the essential host determinant CD55, are recruited to the site of parasite entry and then internalized to the newly formed parasitophorous vacuole membrane. By generating null erythroid cell lines, we showed that one of these proteins, ART4, plays a role in P. falciparum invasion. We also found that genetic variants in both ART4 and AQP1 are associated with susceptibility to the disease in a malaria-endemic population., (© 2021. The Author(s).)
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- 2021
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18. A Comprehensive Gender-related Secretome of Plasmodium berghei Sexual Stages.
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Grasso F, Mochi S, Fratini F, Olivieri A, Currà C, Siden Kiamos I, Deligianni E, Birago C, Picci L, Pizzi E, Pace T, and Ponzi M
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- Animals, Erythrocytes metabolism, Erythrocytes parasitology, Female, Gametogenesis, Germ Cells metabolism, Male, Mice, Proteomics, Subcellular Fractions metabolism, Transport Vesicles metabolism, Life Cycle Stages physiology, Plasmodium berghei growth & development, Plasmodium berghei metabolism, Proteome metabolism, Protozoan Proteins metabolism
- Abstract
Plasmodium , the malaria parasite, undergoes a complex life cycle alternating between a vertebrate host and a mosquito vector of the genus Anopheles In red blood cells of the vertebrate host, Plasmodium multiplies asexually or differentiates into gamete precursors, the male and female gametocytes, responsible for parasite transmission. Sexual stage maturation occurs in the midgut of the mosquito vector, where male and female gametes egress from the host erythrocytes to fuse and form a zygote. Gamete egress entails the successive rupture of two membranes surrounding the parasite, the parasitophorous vacuole membrane and the erythrocyte plasma membrane. In this study, we used the rodent model parasite Plasmodium berghei to design a label-free quantitative proteomic approach aimed at identifying gender-related proteins differentially released/secreted by purified mature gametocytes when activated to form gametes. We compared the abundance of molecules secreted by wild type gametocytes of both genders with that of a transgenic line defective in male gamete maturation and egress. This enabled us to provide a comprehensive data set of egress-related molecules and their gender specificity. Using specific antibodies, we validated eleven candidate molecules, predicted as either gender-specific or common to both male and female gametocytes. All of them localize to punctuate, vesicle-like structures that relocate to cell periphery upon activation, but only three of them localize to the gametocyte-specific secretory vesicles named osmiophilic bodies. Our results confirm that the egress process involves a tightly coordinated secretory apparatus that includes different types of vesicles and may put the basis for functional studies aimed at designing novel transmission-blocking molecules., Competing Interests: Conflict of interest—Authors declare no competing interests., (© 2020 Grasso et al.)
- Published
- 2020
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19. Extended Sedentary Time Increases the Risk of All-Cause Death and New Cardiovascular Events in Patients With Diabetic Kidney Disease.
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Tamiya H, Tamura Y, Mochi S, Akazawa Y, Mochi Y, Banba N, Nakatani Y, Hoshiai M, Ueno A, Nagao M, Tomoe T, Onozaki M, Uema A, Kawabe A, Sugiyama T, and Yasu T
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- Aged, Aged, 80 and over, Diabetes Mellitus, Female, Humans, Male, Middle Aged, Mortality, Prospective Studies, Risk Factors, Cardiovascular Diseases etiology, Diabetic Nephropathies complications, Sedentary Behavior, Stroke etiology
- Abstract
Background: Sedentary behavior may be an independent risk factor for cardiovascular events. This study aimed to clarify the effects of extended sedentary time in patients with diabetic kidney disease (DKD) on the risk of all-cause death and new events.Methods and Results:A prospective cohort study was performed over 39 months. The study included 173 patients with DKD who completed the International Physical Activity Questionnaire (IPAQ) (101 men; mean age, 71±11 years); 37 patients (21.4%) were diagnosed with cardiovascular disease (CVD). New events were defined as all-cause death, cerebral stroke, or CVD requiring hospitalization or commencing hemodialysis (HD). Data were analyzed using a multivariate Cox proportional hazard regression model with variables, including sedentary time. There were 34 cases of new events during the observation period, including 4 cases of stroke, 20 cases of CVD, 4 cases of HD implementation, and 6 cases of death. Hazard ratio (HR) calculations for the new event onset group identified sedentary time as a significant independent variable. The independent variable that was identified as a significant predictor of new events was the sedentary time (60 min/day; HR: 1.23, 95% CI: 1.05-1.45, P=0.012)., Conclusions: Extended sedentary time increased the risk of new cardiovascular or renal events and/or all-cause death in patients with DKD.
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- 2020
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20. Plasmodium berghei Gamete Egress Protein is required for fertility of both genders.
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Andreadaki M, Pace T, Grasso F, Siden-Kiamos I, Mochi S, Picci L, Bertuccini L, Ponzi M, and Currà C
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- Animals, Anopheles parasitology, Erythrocytes parasitology, Female, Gene Knockout Techniques, Malaria parasitology, Male, Mice, Plasmodium berghei genetics, Plasmodium berghei metabolism, Protozoan Proteins metabolism, Gametogenesis genetics, Germ Cells growth & development, Malaria transmission, Plasmodium berghei growth & development, Protozoan Proteins genetics
- Abstract
Male and female Plasmodium gametocytes ingested by the Anopheles mosquitoes during a blood meal egress from the red blood cells by rupturing the two surrounding membranes, the parasitophorous vacuole and the red blood cell membranes. Proteins of the so-called osmiophilic bodies, (OBs), secretory organelles resident in the cytoplasm, are important players in this process. Once gametes emerge, the female is ready to be fertilized while the male develops into motile flagellar gametes. Here, we describe the function(s) of PBANKA_1115200, which we named Gamete Egress Protein (GEP), a protein specific to malaria parasites. GEP is restricted to gametocytes, expressed in gametocytes of both genders and partly localizes to the OBs. A mutant lacking the protein shows aberrant rupture of the two surrounding membranes, while OBs discharge is delayed but not aborted. Moreover, we identified a second function of GEP during exflagellation since the axonemes of the male flagellar gametes were not motile. Genetic crossing experiments reveal that both genders are unable to establish infections in mosquitoes and thus the lack of GEP leads to a complete block in Plasmodium transmission from mice to mosquitoes. The combination of our results reveals essential and pleiotropic functions of GEP in Plasmodium gametogenesis., (© 2020 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.)
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- 2020
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21. Successful management with fosfomycin + ceftazidime of an infection caused by multiple highly-related subtypes of multidrug-resistant and extensively drug-resistant KPC-producing Serratia marcescens.
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Rodríguez C, Brengi S, Cáceres MA, Mochi S, Viñas MR, Rizza CA, Merletti G, Bru E, Assa JD, Raya RR, and Centrón D
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- Drug Resistance, Bacterial, Drug Therapy, Combination, Humans, Microbial Sensitivity Tests, Serratia Infections microbiology, Serratia marcescens drug effects, Serratia marcescens isolation & purification, Treatment Outcome, beta-Lactamases genetics, Anti-Bacterial Agents administration & dosage, Ceftazidime administration & dosage, Fosfomycin administration & dosage, Serratia Infections drug therapy, Serratia marcescens enzymology, beta-Lactamases metabolism
- Published
- 2018
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22. HPV and Chlamydia trachomatis coinfection in women with Pap smear abnormality: baseline data of the HPV Pathogen ISS study.
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Mancini F, Vescio F, Mochi S, Accardi L, di Bonito P, and Ciervo A
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- Adult, Female, Humans, Middle Aged, Uterine Cervical Neoplasms virology, Young Adult, Chlamydia Infections complications, Chlamydia trachomatis, Coinfection, Papanicolaou Test, Papillomaviridae, Papillomavirus Infections complications, Papillomavirus Infections pathology, Uterine Cervical Neoplasms complications, Uterine Cervical Neoplasms pathology
- Abstract
Chlamydia trachomatis (Ct) and human papillomavirus (HPV) are the most common sexually transmitted pathogens. Whereas it is well known that infection with oncogenic HPV genotypes increases the risk of cervical cancer (CC), the implication of Ct in the pathogenesis of CC is still controversial. Hence, to investigate the possible implication of Ct infection alone, or with concomitant HPV infection, in the severity of cervical lesions, we conducted a study in 164 Caucasian HIV-negative women with abnormal Pap. Genomic HPV and Ct DNA were detected in 97 (59%) and 16 (10%) women respectively, and 15 (9%) of women were infected by both. Of the HPV positive samples, 89 (79%) were HR-HPV types or probable HR types and HPV16 was the most represented genotype. Interestingly, it was observed that co-infection was more frequent than HPV infection alone in women with high grade lesions.
- Published
- 2018
23. An Integrated Approach to Explore Composition and Dynamics of Cholesterol-rich Membrane Microdomains in Sexual Stages of Malaria Parasite.
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Fratini F, Raggi C, Sferra G, Birago C, Sansone A, Grasso F, Currà C, Olivieri A, Pace T, Mochi S, Picci L, Ferreri C, Di Biase A, Pizzi E, and Ponzi M
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- Animals, Cholesterol chemistry, Cholesterol metabolism, Computer Simulation, Detergents chemistry, Disease Models, Animal, Gametogenesis physiology, Humans, Lipids analysis, Membrane Microdomains chemistry, Mice, Mice, Inbred BALB C, Proteomics, Sphingolipids chemistry, Sphingolipids metabolism, Life Cycle Stages physiology, Malaria parasitology, Membrane Microdomains metabolism, Plasmodium berghei growth & development, Plasmodium berghei metabolism
- Abstract
Membrane microdomains that include lipid rafts, are involved in key physiological and pathological processes and participate in the entry of endocellular pathogens. These assemblies, enriched in cholesterol and sphingolipids, form highly dynamic, liquid-ordered phases that can be separated from the bulk membranes thanks to their resistance to solubilization by nonionic detergents. To characterize complexity and dynamics of detergent-resistant membranes of sexual stages of the rodent malaria parasite Plasmodium berghei , here we propose an integrated study of raft components based on proteomics, lipid analysis and bioinformatics. This analysis revealed unexpected heterogeneity and unexplored pathways associated with these specialized assemblies. Protein-protein relationships and protein-lipid co-occurrence were described through multi-component networks. The proposed approach can be widely applied to virtually every cell type in different contexts and perturbations, under physiological and/or pathological conditions., (© 2017 by The American Society for Biochemistry and Molecular Biology, Inc.)
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- 2017
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24. Polyclonal dissemination of KPC-2 in Serratia marcescens, including a clone with epidemic behaviour in the nosocomial niche.
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Rodríguez C, Brengi S, Cáceres MA, Mochi S, Viñas MR, Merletti G, Raya RR, and Centrón D
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- Argentina epidemiology, Cross Infection epidemiology, Disease Outbreaks, Drug Resistance, Multiple, Bacterial drug effects, Humans, Serratia Infections epidemiology, Serratia marcescens isolation & purification, Serratia marcescens pathogenicity, Cross Infection microbiology, Drug Resistance, Multiple, Bacterial genetics, Serratia Infections microbiology, Serratia marcescens genetics, beta-Lactamases genetics
- Published
- 2017
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25. [160 activities analyzed by the risk of biomechanical overload on the upper limbs in small industry, handicrafts, services and agriculture].
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Caselli U, Breschi C, Compagnonil R, De Filippo L, Gogliettino MA, Guerrera E, Mameli M, Mastrominico E, Mochi S, and Sarto D
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- Agricultural Workers' Diseases epidemiology, Agricultural Workers' Diseases etiology, Agricultural Workers' Diseases prevention & control, Agriculture, Biomechanical Phenomena, Checklist, Cumulative Trauma Disorders epidemiology, Cumulative Trauma Disorders etiology, Cumulative Trauma Disorders prevention & control, Humans, Industry, Italy, Lifting adverse effects, Occupational Diseases etiology, Occupational Diseases prevention & control, Risk, Weight-Bearing, Arm physiology, Mechanical Phenomena, Occupational Diseases epidemiology, Occupations, Risk Assessment, Stress, Mechanical
- Abstract
We analyzed 160 work activities in the sectors of small industries, handicrafts, services and agriculture to evaluate the risk of biomnechanical overload of the upper limbs with the OCRA Checklist. The aim was to provide a tool available for the risk assessment in activities generally underestimnated for this risk and typical of the Italian production. The information was provided in the form of cards containing easily accessible data, risk scenarios in different timing, along with some of the key prevention interventions that can be realized.
- Published
- 2014
26. [First case of meningitis by Streptococcus suis in the norwest area of Argentina].
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Núñez JM, Marcotullio M, Rojas A, Acuña L, Cáceres M, and Mochi S
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- Acute Disease, Agricultural Workers' Diseases diagnosis, Argentina, Humans, Male, Meningitis, Bacterial diagnosis, Middle Aged, Streptococcal Infections diagnosis, Agricultural Workers' Diseases microbiology, Meningitis, Bacterial microbiology, Streptococcal Infections microbiology, Streptococcus suis isolation & purification
- Abstract
Streptococcus suis causes meningitis, bacteremia, endocarditis, endophthalmitis, artrithis and toxic shock in both pigs and human beings. The incidence of this pathogen is increasing worldwide. A case of 54 years old men from a rural zone of Tucumán, Argentina with two days of headache, vomit and photophobia was admitted septic, with purulent meningitis. Streptococcus suis was growth from the blood and cerebrospinal fluid culture. The patient was treated with ceftriaxona and had a good evolution without sequels. This is the first case of S. suis described in the northwest of Argentina and the third of Latin America, and it is emphasized that the occupational contact with pigs is an important background for the initial suspect of this pathogen.
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- 2013
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27. Successful therapeutic vaccination with integrase defective lentiviral vector expressing nononcogenic human papillomavirus E7 protein.
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Grasso F, Negri DR, Mochi S, Rossi A, Cesolini A, Giovannelli A, Chiantore MV, Leone P, Giorgi C, and Cara A
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- Animals, CD8-Positive T-Lymphocytes immunology, CD8-Positive T-Lymphocytes metabolism, CD8-Positive T-Lymphocytes virology, Calreticulin biosynthesis, Calreticulin genetics, Calreticulin immunology, Cancer Vaccines therapeutic use, Cell Line, Tumor, Female, Gene Expression, Genetic Vectors, Humans, Immunity, Cellular, Immunity, Humoral, Interferon-gamma metabolism, Kaplan-Meier Estimate, Lentivirus enzymology, Mice, Mice, Inbred C57BL, Papillomavirus E7 Proteins biosynthesis, Papillomavirus E7 Proteins immunology, Papillomavirus Infections immunology, Papillomavirus Infections pathology, Papillomavirus Infections virology, Recombinant Fusion Proteins biosynthesis, Recombinant Fusion Proteins genetics, Recombinant Fusion Proteins immunology, Tumor Burden, Uterine Cervical Neoplasms immunology, Uterine Cervical Neoplasms pathology, Uterine Cervical Neoplasms virology, Vaccination, Xenograft Model Antitumor Assays, Cancer Vaccines administration & dosage, Integrases genetics, Lentivirus genetics, Papillomavirus E7 Proteins genetics, Papillomavirus Infections prevention & control, Uterine Cervical Neoplasms prevention & control
- Abstract
Persistent infection with high risk genotypes of human papillomavirus (HPV) is the cause of cervical cancer, one of most common cancer among woman worldwide, and represents an important risk factor associated with other anogenital and oropharyngeal cancers in men and women. Here, we designed a therapeutic vaccine based on integrase defective lentiviral vector (IDLV) to deliver a mutated nononcogenic form of HPV16 E7 protein, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer, fused to calreticulin (CRT), a protein able to enhance major histocompatibility complex class I antigen presentation (IDLV-CRT/E7). Vaccination with IDLV-CRT/E7 induced a potent and persistent E7-specific T cell response up to 1 year after a single immunization. Importantly, a single immunization with IDLV-CRT/E7 was able to prevent growth of E7-expressing TC-1 tumor cells and to eradicate established tumors in mice. The strong therapeutic effect induced by the IDLV-based vaccine in this preclinical model suggests that this strategy may be further exploited as a safe and attractive anticancer immunotherapeutic vaccine in humans., (Copyright © 2012 UICC.)
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- 2013
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28. Anti-tumor CD8+ T cell immunity elicited by HIV-1-based virus-like particles incorporating HPV-16 E7 protein.
- Author
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Di Bonito P, Grasso F, Mochi S, Petrone L, Fanales-Belasio E, Mei A, Cesolini A, Laconi G, Conrad H, Bernhard H, Dembek CJ, Cosma A, Santini SM, Lapenta C, Donati S, Muratori C, Giorgi C, and Federico M
- Subjects
- Adaptive Immunity, Animals, Cell Line, Cross-Priming, HIV-1 immunology, Human papillomavirus 16 immunology, Humans, Membrane Glycoproteins immunology, Mice, Mice, Inbred C57BL, Papillomavirus E7 Proteins, Papillomavirus Infections prevention & control, Papillomavirus Vaccines immunology, Recombinant Fusion Proteins immunology, Vesicular stomatitis Indiana virus immunology, Viral Envelope Proteins immunology, CD8-Positive T-Lymphocytes immunology, Cancer Vaccines immunology, Oncogene Proteins, Viral immunology, nef Gene Products, Human Immunodeficiency Virus immunology
- Abstract
Here we report a novel strategy for the induction of CD8(+) T cell adaptive immune response against viral and tumor antigens. This approach relies on high levels of incorporation in HIV-1 VLPs of a mutant of HIV-1 Nef (Nef(mut)) which can act as anchoring element for foreign proteins. By in vitro assay, we found that VLP-associated Nef(mut) is efficiently cross-presented by antigen presenting cells. Inoculation in mice of VLPs incorporating the HPV-16 E7 protein fused to Nef(mut) led to an anti-E7 CD8(+) T cell response much stronger than that elicited by E7 recombinant protein inoculated with incomplete Freund's adjuvant and correlating with well-detectable anti-E7 CTL activity. Most relevantly, mice immunized with Nef(mut)-E7 VLPs developed a protective immune response against tumors induced by E7 expressing tumor cells. These results make Nef(mut) VLPs a promising candidate for new vaccine strategies focused on the induction of CD8(+) T cell immunity.
- Published
- 2009
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- View/download PDF
29. Clinical and epidemiological correlates of antibody response to human papillomaviruses (HPVs) as measured by a novel ELISA based on denatured recombinant HPV16 late (L) and early (E) antigens.
- Author
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Giorgi C, Di Bonito P, Grasso F, Mochi S, Accardi L, Donà MG, Branca M, Costa S, Mariani L, Agarossi A, Ciotti M, and Syrjänen K
- Abstract
Background: At present, seroreactivity is not a valuable parameter for diagnosis of Human Papillomavirus (HPV) infection but, it is potentially valuable as marker of viral exposure in elucidating the natural history of this infection. More data are needed to asses the clinical relevance of serological response to HPV., Objectives: The objective was to assess the clinical and epidemiological correlates of HPV-seroreactivity in a cohort of HIV-negative and HIV-positive women., Methods: Seroreactivity of 96 women, evaluated in an ELISA test based on denatured HPV16 late (L) and early (E) antigens, was correlated with their clinical and epidemiological data previously collected for a multi-centre Italian study, HPV-PathogenISS study., Results: No significant correlation was found between HPV DNA detection and seroreactivity. Women, current smokers showed significantly less seroreactivity to L antigens as compared with the non-smokers. HIV-positive women showed significantly less (66.7%) antibody response as compared with HIV-negative women (89.3%), with particularly impaired response to L antigens. Women, HIV-positive and current smokers, showed by far the lowest seroprevalence (33.3%) as compared to 75.9% among all other women (OR = 0.158; 95%CI 0.036-0.695, p = 0.014; Fisher's exact test). Importantly, this association did not loose its significance when controlled for confounding from age (continuous variable) in multivariate analysis or using Mantel-Haenszel test for age-groups., Conclusion: It is tempting to speculate that HIV-positive current smokers comprise a special high-risk group, with highly impaired immunological response that could prevent eradication of persistent HPV infections and thus contribute to development of CIN3/CC.
- Published
- 2008
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30. Serum antibody response to Human papillomavirus (HPV) infections detected by a novel ELISA technique based on denatured recombinant HPV16 L1, L2, E4, E6 and E7 proteins.
- Author
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Di Bonito P, Grasso F, Mochi S, Accardi L, Donà MG, Branca M, Costa S, Mariani L, Agarossi A, Ciotti M, Syrjänen K, and Giorgi C
- Abstract
Background: Human papillomaviruses (HPVs) are the primary etiological agents of cervical cancer and are also involved in the development of other tumours (skin, head and neck). Serological survey of the HPV infections is important to better elucidate their natural history and to disclose antigen determinants useful for vaccine development. At present, the analysis of the HPV-specific antibodies has not diagnostic value for the viral infections, and new approaches are needed to correlate the antibody response to the disease outcome. The aim of this study is to develop a novel ELISA, based on five denatured recombinant HPV16 proteins, to be used for detection HPV-specific antibodies., Methods: The HPV16 L1, L2, E4, E6 and E7 genes were cloned in a prokaryotic expression vector and expressed as histidine-tagged proteins. These proteins, in a denatured form, were used in ELISA as coating antigens. Human sera were collected from women with abnormal PAP smear enrolled during an ongoing multicenter HPV-PathogenISS study in Italy, assessing the HPV-related pathogenetic mechanisms of progression of cervical cancer precursor lesions. Negative human sera were collected from patients affected by other infectious agents. All the HPV-positive sera were also subjected to an avidity test to assess the binding strength in the antigen-antibody complexes., Results: Most of the sera showed a positive reactivity to the denatured HPV16 proteins: 82% of the sera from HPV16 infected women and 89% of the sera from women infected by other HPV genotypes recognised at least one of the HPV16 proteins. The percentages of samples showing reactivity to L1, L2 and E7 were similar, but only a few serum samples reacted to E6 and E4. Most sera bound the antigens with medium and high avidity index, suggesting specific antigen-antibody reactions., Conclusion: This novel ELISA, based on multiple denatured HPV16 antigens, is able to detect antibodies in women infected by HPV16 and it is not genotype-specific, as it detects antibodies also in women infected by other genital HPVs. The assay is easy to perform and has low cost, making it suitable for monitoring the natural history of HPV infections as well as for detecting pre-existing HPV antibodies in women who receive VLP-based HPV vaccination.
- Published
- 2006
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31. Human antibody response to Toscana virus glycoproteins expressed by recombinant baculovirus.
- Author
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Di Bonito P, Bosco S, Mochi S, Accardi L, Ciufolini MG, Nicoletti L, and Giorgi C
- Subjects
- Animals, Antibodies, Viral blood, Antibody Specificity, Baculoviridae genetics, Blotting, Western, Cell Line, Gene Expression Regulation, Viral, Genetic Vectors genetics, Humans, Immune Sera immunology, Molecular Sequence Data, Nucleocapsid Proteins biosynthesis, Nucleocapsid Proteins immunology, Phlebotomus Fever diagnosis, Promoter Regions, Genetic, Recombinant Proteins biosynthesis, Recombinant Proteins immunology, Sandfly fever Naples virus genetics, Spodoptera, Viral Fusion Proteins biosynthesis, Antibodies, Viral immunology, Phlebotomus Fever immunology, Sandfly fever Naples virus immunology, Viral Fusion Proteins immunology
- Abstract
The arthropod-borne Toscana virus has been associated with acute neurological disease in humans. In this study, the viral envelope glycoproteins were expressed in soluble form in a baculovirus system. The recombinant sGN and sGC proteins were used as viral antigens in a Western blot assay to analyze the specific immune response in sera from patients with recognized virus-associated aseptic meningitis. The anti-glycoprotein and the anti-nucleoprotein N IgG responses were compared by an immunoassay based on the recombinant proteins. In this system, all the sera showed a high reactivity to the N protein, but they differed in the response to the glycoproteins., (Copyright 2002 Wiley-Liss, Inc.)
- Published
- 2002
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32. Activity of Toscana and Rift Valley fever virus transcription complexes on heterologous templates.
- Author
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Accardi L, Prehaud C, Di Bonito P, Mochi S, Bouloy M, and Giorgi C
- Subjects
- Animals, Base Sequence, Chlorocebus aethiops, Molecular Sequence Data, RNA, Viral metabolism, Vero Cells, Bunyaviridae genetics, Rift Valley fever virus genetics, Transcription, Genetic
- Abstract
A transcription system for Toscana virus (TOSV) (a member of the family BUNYAVIRIDAE:, genus PHLEBOVIRUS:) was constructed. For in vivo expression, the TOSV transcription system uses the viral N and L proteins and an S-like RNA genome containing the chloramphenicol acetyltransferase reporter gene in the antisense orientation flanked by the viral genomic 5'- and 3'-terminal S sequences. It was found that the N and L proteins represent the minimal protein requirement for an active transcription complex. To investigate the possibility of reassortment between TOSV and Rift Valley fever virus (RVFV), the activity of their polymerase complexes was tested on their heterologous S-like RNA genomes and this showed that both virus complexes were active. Moreover, hybrid transcriptase complexes with protein components originating from the two viruses were tested on both virus templates and only the combination RVFV L + TOSV N on RVFV S-like RNA was found to be active in this assay. These results suggest that virus reassortants might be generated whenever the two viruses infect the same host.
- Published
- 2001
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33. Detection of Toscana virus-specific immunoglobulins G and M by an enzyme-linked immunosorbent assay based on recombinant viral nucleoprotein.
- Author
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Ciufolini MG, Fiorentini C, di Bonito P, Mochi S, and Giorgi C
- Subjects
- Animals, Blotting, Western, Brain virology, Enzyme-Linked Immunosorbent Assay methods, Humans, Mice, Nucleocapsid Proteins immunology, Phlebotomus Fever blood, Phlebotomus Fever immunology, Sensitivity and Specificity, Antibodies, Viral blood, Immunoglobulin G blood, Immunoglobulin M blood, Phlebotomus Fever diagnosis, Phlebovirus immunology, Phlebovirus isolation & purification
- Abstract
An enzyme-linked immunosorbent assay (ELISA) based on the recombinant Toscana virus nucleoprotein (rN) has been developed. Its sensitivity and specificity for the detection of virus-specific immunoglobulins G and M in human sera were similar to those of the ELISA that is based on an antigen extracted from infected mouse brain and that is routinely used for serodiagnosis.
- Published
- 1999
- Full Text
- View/download PDF
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