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MADS-boxgenes play a vital role in the vegetative and reproductive growth of plants. In this study, a MiAGL1gene was cloned and identified from mango (Mangifera indicaL.). The DNA sequence of AGAMOUS-LIKE1(MiAGL1) was 8741 bp in length, including a 723 bp open reading frame and encoding 241 amino acids. MiAGL1belongs to the MADS-box family. This gene was expressed not only in vegetative tissues but also in floral organs, and the highest expression level was found in flowers. MiAGL1was expressed in leaves at different floral developmental stages, but the peak appeared at the floral organ differentiation stage. MiAGL1was present in the cell membrane and nucleus. Ectopic expression of MiAGL1in Arabidopsisresulted in significant early flowering under long-day conditions. Overexpression of MiAGL1resulted in abnormal flowering and silique morphology, such as a decrease or absence of petals, smaller petals, and shorter, bent or distorted capsules. The endogenous Arabidopsis thalianaflowering-related genes FT, AP1, and SEPwere significantly upregulated, and AtSVPwas downregulated in the transgenic lines. Therefore, our data showed that the MiAGL1gene may play a crucial role in flowering time regulation and floral organ identity.