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16 results on '"Mo, Fulei"'

14. Genome-Wide Identification of APX Gene Family in Citrus maxima and Expression Analysis at Different Postharvest Preservation Times.

Author

Zhang, Yu, Peng, Yujiao, Zhang, Huixin, Gao, Qiuyu, Song, Fangfei, Cui, Xueyu, and Mo, Fulei

Subjects
POMELO, GENE families, GENE expression, REACTIVE oxygen species, GRAPEFRUIT
Abstract

Ascorbate peroxidase (APX) is a crucial enzyme involved in cellular antioxidant defense and plays a pivotal role in modulating reactive oxygen species (ROS) levels under various environmental stresses in plants. This study utilized bioinformatics methods to identify and analyze the APX gene family of pomelo, while quantitative real-time PCR (qRT-PCR) was employed to validate and analyze the expression of CmAPXs at different stages of fruit postharvest. This study identified 96 members of the CmAPX family in the entire pomelo genome, with uneven distribution across nine chromosomes and occurrences of gene fragment replication. The subcellular localization includes peroxisome, cytoplasm, chloroplasts, and mitochondria. The CmAPX family exhibits a similar gene structure, predominantly consisting of two exons. An analysis of the upstream promoter regions revealed a significant presence of cis-acting elements associated with light (Box 4, G-Box), hormones (ABRE, TCA-element), and stress-related (MBS, LTR, ARE) responses. Phylogenetic and collinearity analyses revealed that the CmAPX gene family can be classified into three subclasses, with seven collinear gene pairs. Furthermore, CmAPXs are closely related to citrus, pomelo, and lemon, followed by Arabidopsis, and exhibit low homology with rice. Additionally, the transcriptomic heat map and qPCR results revealed that the expression levels of CmAPX57, CmAPX34, CmAPX50, CmAPX4, CmAPX5, and CmAPX81 were positively correlated with granulation degree, indicating the activation of the endogenous stress resistance system in pomelo cells by these genes, thereby conferring resistance to ROS. This finding is consistent with the results of GO enrichment analysis. Furthermore, 38 miRNAs were identified as potential regulators targeting the CmAPX family for post-transcriptional regulation. Thus, this study has preliminarily characterized members of the APX gene family in pomelo and provided valuable insights for further research on their antioxidant function and molecular mechanism. [ABSTRACT FROM AUTHOR]

Published
2024
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16. Genome-wide identification and expression analysis of the SlNACgene family in tomato based on a high-quality genome

Author

Chen, Xiuling, Mo, Fulei, Shen, Chunyang, Meng, Lingjun, Zou, Yuxin, Xue, Xiaopeng, Cheng, Mozhen, Meng, Fanyue, Qi, Haonan, and Wang, Aoxue

Abstract

NAC proteins in plants respond to stress and play an important role in plant growth regulation. This regulation occurs through a variety of downstream effects mediated by plant hormones in response to stress. In this study, we performed a systematic genome-wide analysis of the NACgene family in tomato based on genome version SL4.0. We identified 99 SlNACgenes by abiotic stress analysis of conserved motifs and gene structure, phylogenetic analysis, cis-element analysis, chromosome localization analysis, synteny relationship and expression pattern analysis. These 99 SlNACgenes were distributed on all 12 chromosomes. Except for genes without introns, there was no significant correlation between gene structures and phylogenetic relationships. Most of the cis-elements identified were associated with plant hormones and environmental responses. There were 77 SlNACgenes that showed no homogeneity between tomato and wild rice, suggesting that these homologous genes arose after the differentiation of dicotyledonous and monocotyledonous plants. The comprehensive analysis of the SlNACgene family provides a valuable resource for studying this gene family in tomato and a foundation for further study of the functional characteristics of these genes.

Published
2022
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