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The environmental and faunistic research conducted included defining the composition and distribution of caddisflies collected using ultraviolet (UV) light trap at 11 stations along the Cetina River, from the spring to the mouth, and also along its tributaries the Ruda River and the Grab River with two sampling stations each, and the Rumin River with one station. The research was conducted in the period from August 2004 until August 2005 on the Cetina and the Ruda rivers, and from April 2007 to September 2007 on the Grab and the Rumin rivers. A total of 71 caddisfly species and 10,048 individuals were recorded in the study area. Results of the composition and structure of caddisflies were processed with statistical methods using the NMDS analysis. There were differences in the composition of caddisflies in the upper, middle and lower reaches. The paper also presents some ecological features of caddisflies, such as seasonal dynamics and diversity of caddisflies communities. Results of this work contribute to faunistic and ecological knowledge of Croatian caddisflies.

Adult caddisflies were collected during 2018 and 2019 from Kožuf and Baba mountains, located in the southern end of the Republic of North Macedonia. A total of 36 species were recorded, of which 22 and 8 were recorded for the first time for Kožuf and Baba mountains, respectively. Limnephilus flavicornis (Fabricius, 1787) is reported for the first time from the country from Kožuf Mountain. In addition, new localities of several rare and endemic caddisfly species were found. The results of this study highlight Kožuf and Baba mountains as refugium for caddisfly species of conservation importance.

A new alien psyllid Glycaspis brimblecombei, native in Australia, was first discovered in Croatia in 2020. Its presence was also confirmed in the Botanical Garden of the Institute for Marine and Coastal Research (University of Dubrovnik) on the island of Lokrum, using DNA barcoding. Distribution and spreading of this alien species were noted as across the other continents so in Europe. The pest is considered as a threat to forestry, paper industry and could affect ornamental values of the eucalyptus species. It is important to continue with following research on this species because of the potential spreading and ascendant estimation.

The paper provides the results of DNA barcoding based on the cytochrome c oxidase subunit 1 mitochondrial gene (mtCOI) of 110 Trichoptera specimens collected in 36 springs in the PannonianPeripannonian, central mountainous and Mediterranean part of Croatia. We barcoded 70 species from 32 genera and 15 families. The data obtained show interesting faunistic and taxonomic results, for, for example, the species Rhyacophila cabrankensis, R. balcanica, Crunoecia kempnyi, Allogmaus auricollis and emphasize the need for further faunistic research into springs, in their role as habitats with a specific and very interesting fauna. The mtCOI DNA barcoding should be included in such research, because it would enable better presentation of the results, especially regarding biodiversity, taxonomy, phylogeny and conservation biology, not just as a segment of a local but also of a global process of understanding biodiversity in a different way. The results of this study show a global need for the protection of springs, because they are specific not only as habitats, but also as localities with an interesting fauna and often endemic species of very limited distribution (for example Rhyacophila cabrankensis)., U radu se prikazuju rezultati DNA barkodiranja temeljenog na mitohondrijskom genu za podjedinicu 1 citokrom c oksidaze (mtCOI), za 110 primjeraka Trichoptera prikupljenih u 36 izvora u panonsko-peripanonskom, središnje-planinskom i mediteranskom području Hrvatske. DNA barkodirano je 70 vrsta iz 32 roda i 15 porodica. U studiji se ukazuje na neke zanimljive faunističke i taksonomske rezultate, npr. za vrste Rhyacophila cabrankensis, R. balcanica, Crunoecia kempnyi, Allogmaus auricollis te potrebu daljnjih faunističkih istraživanja izvora kao staništa sa specifičnom i vrlo zanimljivom faunom. U ta istraživanja zbog kvalitetnije prezentacije rezultata, posebno u područjima bioraznolikosti, taksonomiji, filogeniji i konzervacijskoj biologiji, potrebno je uključiti i metodu DNA barkodiranja mtCOI, kao segment ne samo lokalnog, nego i globalnog procesa u spoznavanju bioraznolikosti na jedan drugačiji način. Navedeni rezultati ovog rada ukazuju na globalnu potrebu veće zaštite izvora jer su specifični ne samo kao staništa, nego vrlo često i kao područja nalaza endemskih vrsta s vrlo malim područjem rasprostranjenja (npr. Rhyacophila cabrankensis).

Molecular methods are increasingly being utilized for accurate identification of ticks (Acari: Ixodidae), especially in cases of morphologically highly similar species. In this study, we performed molecular research of the tick fauna in Croatia using DNA barcoding method. Ticks were sampled in three biogeographical regions and thirteen species were recorded: Dermacentor marginatus, Dermacentor reticulatus, Haemaphysalis concinna, Haemaphysalis inermis, Haemaphysalis punctata, Hyalomma marginatum, Ixodes frontalis, Ixodes hexagonus, Ixodes kaiseri, Ixodes ricinus, Rhipicephalus bursa, Rhipicephalus sanguineus s.l. and Rhipicephalus turanicus. Ixodes kaiseri is for the first time recorded in the fauna of Croatia. Of the thirteen hard tick species analyzed in this study, pathogens from different groups (bacteria, protozoa and viruses) have been detected in eight species in Croatia so far. For the important vector species R. sanguineus s.s., new distributional data for Croatia are given. The standard COI barcoding region was amplified, and the sequences were analyzed by species delimitation methods together with the sequences of conspecific and congeneric species from the public BOLD database. Our specimens of H. punctata represent a new, genetically distinct MOTU. A brief overview of the available public DNA barcoding data for Ixodidae is presented, highlighting the need for an integrative approach for the clarification of the taxonomic status of problematic Ixodid taxa. The results provide a basis for the establishment of a molecular data platform for the Ixodidae of the Croatian fauna.

To date, 13 species of Trichoptera from 11 genera and 8 families have been reported for the Adriatic islands of Cres, Krk, Pag and Hvar. Relatively recent research has been conducted on the islands of Cres, Krk and Pag, where 329 adults of Trichoptera belonging to 10 species from 8 genera and 6 families were collected. Most dominant was Ecnomus tenellus with 235 specimens collected (islands of Krk and Cres), followed by Leptocerus tineiformis (Krk) with 51 specimens collected. Only one specimen of the each species Stenophylax mitis (Cres) and Mystacides azurea (Krk) was recorded. Most of the species were recorded on the island of Krk (7), and the lowest on the island of Pag (2). Faunistically most interesting was the first finding of species Stenophylax mitis McL. for the fauna of Croatia., Na području jadranskih otoka (Cres, Krk, Pag, Hvar) utvrđeno je do sada 13 vrsta Trichoptera iz 11 rodova i 8 porodica. Novija istraživanja provedena su na otocima Cresu, Krku i Pagu u kojima je prikupljeno 329 adultnih oblika Trichoptera s 10 vrsta iz 8 rodova i 6 porodica. Najdominantnije vrste bile su Ecnomus tenellus s 235 prikupljenih primjeraka (Krk i Cres) i Leptocerus tineiformis (Krk) s 51 prikupljenim primjerkom, a samo s jednim primjerkom zabilježene su vrste Stenophylax mitis (Cres) i Mystacides azurea (Krk). Najveći broj vrsta utvrđen je na otoku Krku (7), a najmanji na otoku Pagu (2). Faunistički najzanimljiviji je nalaz vrste Stenophylax mitis McL. koja je ovim istraživanjima utvrđena po prvi puta u fauni Hrvatske.

In this study we describe Rhyacophila siparantum sp. nov., a new species of the Rhyacophila philopotamoides species group from the Bjeshkët e Nemuna Mountains in Kosovo, based on morphological (male adults) and molecular (sequencing of the barcode region of the cytochrome c oxidase subunit I gene (COI)) characteristics. The new species is morphologically closest to Rhyacophila schmidinarica Urbanič, Krušnik & Malicky, 2000, and also closely resembling Rhyacophila hirticornis McLachlan, 1879. R. siparantum sp. nov. differs from both its most similar congeners primarily by the shape of the segment X, which is in lateral view short apically and with a pronounced lateral hump on the posterior edge. This difference, combined with other characters related to the shape of the second segment of inferior appendages, spine pattern of parameres and size of ventral teeth on segments VI and VII, make R. siparantum sp. nov. easily distinguishable from both most similar congeners. Phylogenetic and taxonomic relationships were reconstructed using two methods of phylogenetic inference, and two species delimitation methods. All this supports Rhyacophila siparantum sp. nov. as a distinct taxon. The adults of Rhyacophila siparantum sp. nov. were found during the period May – August, nearby a small rheocrene spring inside a forested area. The new species is most probably a microendemic of the Bjeshkët e Nemuna, a mountainous massive known for several other endemic species of caddisflies. Rhyacophila siparantum sp. nov. is the seventeenth known species of the genus Rhyacophila Pictet, 1834 from Kosovo.

[EN] The presence and distribution of Rhyacophila fasciata Hagen 1859 in Europe were revised, based on bibliographic study, collection specimens, and new material collected in different countries. The status of formerly synonymized species, Rhyacophila ferruginea (Scopoli 1763) and Rhyacophila septentrionis McLachlan 1865 was also assessed. The type of R. ferruginea is missing, the taxon is still unidentified, and thus we propose Rhyacophila ferruginea as a nomen dubium. Morphological features and genetic evidence revealed that R. septentrionis differs from R. fasciata, so we propose to change its status to status resurrectus. We therefore include new descriptions of the different stages (larva, pupa, male, and female) of R. fasciata and of R. septentrionis, together with a molecular analysis based on mitochondrial cytochrome oxidase I (mtCOI) and ecological notes. The species R. coppai Oláh 2020 NEW SYNONYM and R. soreda Coppa & Oláh 2020 NEW SYNONYM are synonyms of R. sociata Navás 1916; the species R. kopasa Oláh & Coppa 2020 NEW SYNONYM and R. rova Oláh & Coppa 2020 NEW SYNONYM are synonyms of R. denticulata McLachlan 1879; the species R. matrensis Oláh & Szcz¿sny 2020 is probably a synonym of R. fasciata, so more study of this species is needed in order to confirm or deny that it is a valid species., [ES] Se ha revisado la presencia y distribución de Rhyacophila fasciata Hagen 1859 en Europa, basada en el estudio de la bibliografía, especímenes de colecciones y en nuevo material recolectado en diferentes países. Se evaluó asimismo el estado de las especies anteriormente sinonimizadas Rhyacophila ferruginea (Scopoli 1763) y Rhyacophila septentrionis McLachlan, 1865. El ejemplar tipo de R. ferruginea se ha perdido y el taxón permanece todavía sin identificar, por lo que proponemos Rhyacophila ferruginea como un nomen dubium. Las características morfológicas y las evidencias genéticas indican que R. septentrionis difiere de R. fasciata, por lo que proponemos un cambio de estatus a status resurrectus. Se incluyen nuevas descripciones de las distintas etapas (larva, pupa, machos y hembras) de R. fasciata y de R. septentrionis, junto con el análisis molecular del gen mitocondrial Citocromo Oxidasa subunidad I (COImt), y notas ecológicas. Las especies R. coppai Oláh 2020 NUEVO SINÓNIMO y R. soreda Coppa & Oláh 2020 NUEVO SINÓNIMO son sinónimos de R. sociata Navás 1916; las especies R. kopasa Oláh & Coppa 2020 NUEVO SINÓNIMO y R. rova Oláh & Coppa 2020 NUEVO SINÓNIMO son sinónimos de R. denticulata McLachlan 1879; la especie R. matrensis Oláh & Szcz¿sny 2020 es probablemente un sinónimo de R. fasciata, por lo que se necesita un estudio más detallado para confirmarla o rechazarla como especie válida., This research is a part of scientific project “DNA barcoding of Croatian faunal biodiversity” (IP-2016-06-9988) funded by the Croatian Science Foundation. The work of P. Chvojka was supported by the Ministry of Culture of the Czech Republic (DKRVO 2019-2023/5.I.b, National Museum, 00023272).

The Balkan endemic caddisfly species Drusus osogovicus Kumanski, 1980 that was previously known only from Bulgaria was recently reported from the Republic of North Macedonia as well. However, all data on the presence of the species included only adult specimens as the larval stage remained unknown. In the following study we present the first description of the larval stage of Drusus osogovicus based on morphological characters. In order to prove the conspecificity between the larvae and the adults, DNA barcoding of the specimens was also conducted. Furthermore, lists of the crucial diagnostic characters present only in D. osogovicus larvae as well as information on the distribution and ecological preferences of the species are also provided.

The subfamily Drusinae (Trichoptera, Limnephilidae) is represented in Croatia with eight (8) species, including Ecclisopteryx asterix Malicky, 1979 recorded for the first time in Croatian fauna. The species was collected on two occasions: March 10th 2015 and March 6th 2021 in the Šumi Spring, at the Ivanščica Mt. in Hrvatsko Zagorje (North Croatia). This new finding considerably extends the distribution of this species, previously considered a microendemic of the Alps. The distribution of the two species, Drusus schmidi Botosaneanu, 1960 and D. croaticus Marinković-Gospodnetić, 1971 has been also enlarged with new findings. Within this study, nine specimens of the aforementioned species have been DNA barcoded and sequences were entered into the BOLD database. The results of this paper contribute to the ecological and faunistic knowledge of caddisflies in Croatia, with an emphasis on the subfamily Drusinae.

Global climate change and the accompanying rise in temperature could affect the biology and ecology of a number of vectors, including mosquitoes. High altitude areas that were previously unsuitable for the spread of mosquito vector populations could become suitable. The aim of this research was to study the distribution of mosquito species in higher altitude regions of Croatia. Samples were collected in three areas: Slavonian Mountains, Gorski Kotar, and Middle Velebit. Specimens were morphologically determined and confirmed by DNA barcoding and other genetic markers and showed the presence of 16 species belonging to six genera. The most abundant species were the Culex pipiens complex with 50% of the collected specimens. Both pipiens (Linnaeus, 1758) and molestus (Forskal, 1775) biotypes and their hybrids were identified within the complex, followed by Culex torrentium (Martini, 1925) (20.2%), Culiseta longiareolata (Macquart, 1838) (8.5%), and the invasive species Aedes japonicus (Theobald, 1901) (7.8% of the total number of collected specimens). The remaining 12 species made up 14.7% of the collected specimens. Intraspecific COI p-distances were within the standard barcoding threshold for OTUs, while interspecific genetic distances were much higher, confirming the existence of barcoding gaps. Mosquito fauna of Croatian mountains showed a moderate variety and made 30.8% of the total number of recorded mosquito species in Croatia thus far.

Natural history museum collections can be a helpful tool in documenting changes in biodiversity throughout decades or even centuries. This article uncovers information on the content of three different museum collections, collected over 100 years. It deepens the knowledge on the distribution of pyraloid moth species occurring predominantly in the Continental parts of Croatia, but also in other areas of this country. The article presents the first published listing of pyraloid moth species held in three museum collections of the Croatian Natural History Museum in Zagreb. The list contains 148 taxa in total, 61 from the Igalffy collection, 96 from the Koča collection, and 96 from the Kučinić collection. Altogether, 96 species from the family Crambidae and 52 species from the family Pyralidae are listed.

[EN] We present the description of two new subspecies of the Rhyacophila fasciata Group: Rhyacophila fasciata delici Ku¿ini¿ & Valladolid (ssp. nov.), broadly distributed in Croatia and present also in Bosnia and Herzegovina, and R. fasciata viteceki Valladolid & Ku¿ini¿ (ssp. nov.), found in Bosnia and Herzegovina. Our study of the morphology of adults, as well as our analysis of the barcode region of the mitochondrial cytochrome oxidase I (mtCOI) gene and geographical distribution confirm the differences of the two new subspecies with the nominal species R. f. fasciata, also found in both countries., [ES] Se presenta la descripción de dos nuevas subespecies del Grupo Rhyacophila fasciata: Rhyacophila fasciata delici Kučinić& Valladolid (ssp. nov.), ampliamente distribuida en Croacia y presente también en Bosnia y Herzegovina y R. fasciata vitecekiValladolid & Kučinić (ssp. nov.), encontrada en Bosnia y Herzegovina. El estudio de la morfología de los adultos, así como el análisis de la citocromo oxidasa I mitocondrial (COImit) y la distribución geográfica confirma las diferencias de las dos nuevas subespecies con la especie nominal R. f. fasciata, también presente en ambos países., This research is a part of scientific project “DNA barcoding of Croatian faunal biodiversity” (IP–06–2016–9988) funded by the Croatian Science Foundation.

The montane species Catoptria acutangulellus (Herrich-Schäffer, 1847) was reconfirmed from Croatia on Mt Biokovo after almost 100 years. Previous historical records were published in 1850 from Mt Biokovo and 1896 from the area around Rijeka. There are no recently published records of this species, whether from Mt Biokovo or any other Croatian mountain. Therefore, these new records also represent the first findings of this species for Mt Biokovo in more than 140 years. Habitat loss or habitat change due to global warming could be a threat to montane species such as C. acutangulellus. Since the only known localities of C. acutangulellus in Croatia are the highest parts of Mt Biokovo, in the future it could be a threatened species in the country. Altogether 15 specimens of C. acutangulellus were collected during a field trip to Mt Biokovo, on 20th August 2019. Later on, another 15 specimens were discovered from the same area in the Kučinić Collection from CNHM in Zagreb. All specimens were collected by the second author in the 1990s. These findings reconfirm the presence of C. acutangulellus in the fauna of Croatia and provide evidence that this species is still present on Mt Biokovo., Nakon skoro 100 godina na planini Biokovu je za Hrvatsku potvrđena planinska vrsta Catoptria acutangulellus (Herrich-Schäffer, 1847). Prethodni nalazi potječu iz 1850. s Biokova i iz 1896. godine iz okolice Rijeke. Ne postoje recentni objavljeni nalazi za ovu vrstu, niti s Biokova, niti nekog drugog područja Hrvatske. Osim toga ovi novi nalazi predstavljaju prve nalaze za područje planine Biokovo nakon više od 140 godina. Nestanak staništa ili promjene na staništima nastale uslijed globalnog zatopljenja mogli bi biti prijetnja planinskim vrstama kao što je C. acutangulellus. S obzirom na to da su jedina poznata nalazišta ove vrste najviši dijelovi Biokova, u budućnosti bi ova vrsta mogla biti ugrožena u Hrvatskoj. Tijekom terenskog istraživanja 20. kolovoza 2019. godine na Biokovu je ulovljeno ukupno 15 primjeraka vrste C. acutangulellus. U nastavku rada je također otkriveno 15 primjeraka s istog područja pohranjenih u Zbirci Kučinić u HPM-u u Zagrebu, prikupljenih 90-tih godina 20. st. Ovi dodatni nalazi potvrđuju prisustvo vrste C. acutangulellus u fauni Hrvatske te ukazuju na to da je ta vrsta i dalje prisutna na području planine Biokovo.

In this paper new data on distribution and new taxonomic status of the caddisfly Triaenodes ochreellus lefkas are given. DNA barcoding data are also included into defining new status of the species Triaenodes lefkas stat. nov. Data from DNA barcoding analyses of 60 specimens from the genus Triaenodes from the BOLD database show certain taxonomic peculiarities in specimens of T. unanimis from Japan.

Caddisfly faunistic and ecological features were studied at 14 sampling sites along the catchment area of the Dobra River. Adults were collected monthly, over a period of 13 months (from early November 2009 until the end of November 2010), using ultraviolet (UV) light trap and entomological net. Physico-chemical parameters of water and air were also measured at all sampling sites. The study encompassed several segments of caddisfly fauna: the composition, new records, the distribution of all recorded species and seasonal dynamics. A total number of 77 species were recorded, out of which 15 species and four genera were new records for Croatian caddisfly fauna. The CCA analysis showed that the water temperature and the altitude had the highest impact on distribution of caddisfly fauna in this area. The results of this study contribute to the increase of faunistic and ecological knowledge of the Croatian caddisflies.

This study presents new insights into ecological traits of water beetles in lotic karst habitats of the Eastern Mediterranean. The aims of the study were to investigate population aspects and ecological traits of water beetles. Data were collected monthly during one year from a large perennial river showing current anthropogenic disturbance. Altogether 11 families, 23 genera and 31 species were identified. Elmidae were the most diverse and abundant family, and the genus Elmis predominated at all sampling sites. The distribution of water beetles along the river was influenced primarily by river bed morphology and beetle substrate preference. Water beetles were more abundant in bryophytes but more diverse in angiosperms. Ecological traits of water beetles along the watercourse were defined primarily by alkalinity and current velocity. Larval stages correlated with water depth and current velocity, while the adult stage correlated only with water depth. Our results support the potential of water beetles as indicators of water quality. This report provides the most detailed investigation so far of water beetles in the Eastern Mediterranean region, providing a basis for further detailed studies of water beetles and their ecology in freshwater habitats in southeastern Europe.

Even though alderflies, as well as many other aquatic macroinvertebrates, are widely used as indicators of freshwater quality, the knowledge of their distribution, biology and ecology in karst freshwater habitats is still very scarce. Aiming to fill this gap, we investigated alderfly assemblages along an oligotrophic karst hydrosystem. Sampling was carried out on a monthly basis over a one-year period (2007/2008) in different types of freshwater habitats and microhabitats. Our goals were to determine population aspects, habitat and microhabitat preferences of alderflies as well as their relation to environmental factors. Due to the specificity of karst freshwater habitats, alderflies were rather rare in the studied hydrosystem. Yet, their fauna was diverse with four identified species, where Sialis fuliginosa Pictet, 1836 was the most widespread one. Sialis sordida (Klingstedt, 1932) was recorded for the first time in the Balkan Peninsula being the rarest species in our study. Substrate type and water velocity showed as key environmental parameters in determining alderfly assemblages. Alderflies were mostly recorded from a canyon type mountainous stream, and silt with leaf litter, respectively. They were not recorded at sites characterized by predominately solid stones (i.e. cobbles) and mosses, and with intermittent water flow. Our results strongly indicate that alderflies could be used in future conservation and protection management procedures in karst freshwater habitats.

The paper brings data about the new findings of the rare Mediterranean caddisfly species Micropterna wageneri Mal. in Croatia with first data of DNA barcoding for this species. The species is recorded in Konavle region and on the Mt. Biokovo. We also present the diversity of the genus Micropterna in Croatia and discuss issues related to the DNA barcoding for this genus in Croatia and Europe.

This paper presents the distribution of two species of the genus Ecclisopteryx in Croatia based on literature data and the new records: E. keroveci and E. ivkae. The species E. ivkae was recorded at eight sites in the catchment area of the Cetina River, while E. keroveci occurs within the catchment area of the Kupa River and along the Veličanka River. Due to the restricted distribution, we proposed conservation and protection measures for both species.

In this study we present: two species of caddisflies new for Croatian fauna from the family Hydroptilidae (Hydroptila simulans Mosley, Orthotrichia costalis Curtis), first DNA barcoding of caddisfly species in the Krka National Park and a discussion about recorded caddisfly fauna in the Krka NP from this study. From a faunistic point of view several species are interesting: Hydropsysche mostarensis Klapálek, Hydroptila simulans Mosely, Hydroptila forfcipata Eaton, Orthotrichia costalis Curtis and Tinodes pallidulus McLachlan. For the species Oecetis notata Rambur we have recorded interesting taxonomical remarks. Furthermore, within this study we used DNA barcoding which showed to be a very good and useful method for identification of very small and morphologically similar species from the family Hydroptilidae, or females from the family Psychomyiidae., U ovome radu prikazujemo: dvije nove vrste zabilježene u fauni Hrvatske iz porodice Hydroptilidae (Hydroptila simulans Mosley, Orthotrichia costalis Curtis), prikaz DNA barkodiranja tulara u Nacionalnom parka Krka te osvrt na faunistički najzanimljivije vrste utvrđene tijekom ovih istraživanja. Faunistički najzanimljiviji su nalazi sljedećih vrsta: Hydropsysche mostarensis Klapálek, Hydroptila simulans Mosely, Hdroptila forfcipata Eaton, Orthotrichia costalis Curtis i Tinodes pallidulus McLachlan. Vrsta Oecetis notata Rambur nazanimljivija je s taksonomskog aspekta. U ovim istraživanjima metoda DNA barkodiranja pokazala se kao vrlo korisna u determinaciji vrlo malih i morfološki sličnih vrsta iz porodice Hydroptilidae te ženki iz porodice Psychomyiidae.

In Europe the Phyrganeidae family comprises 7 genera and 20 species. In Croatia, 6 species have been recorded from this family, and four of them recorded and collected in the last five years have been DNA barcoded. In terms of faunistic research, most interesting is the record of Trichostegia minor Curtis, 1834 from the region of the Gacka River in Lika, in upland Croatia. Up to now four species have been included in the BOLD system with 6 DNA-barcoded specimens. From the Gacka River area, 10 specimens of 8 species, including T. minor, have been barcoded and included into the BOLD system. Phylogenetic research based on DNA barcode data has shown some interesting results. Specimen of T. minor collected in the area of the Gacka River is clustered with a specimen of this species from Austria into a separate subgroup, which shows minor molecular specifics. Phylogenetic analysis has confirmed the justification of the status of a subspecies from Mongolia, Phryganea grandis rotundata Ulmer, 1905. Future Trichoptera fauna research in Croatia will continue investigations of the family Phryganeidae in various parts of the country for the purpose of ascertaining the detailed distribution of all the species recorded, to enable finding some other previously missing species, as well as collecting and DNA barcoding of Hagenella clathrata (Kolenati, 1848) and Oligostomis reticulata (Linnaeus, 1761). With respect to these species, the data will also be useful for the area of conservation biology, necessary for their protection and for the protection of the sites in which they occur, for it is clear that these are very rare species in Croatia, T. minor also belonging to this group: 50% of the species from this family recorded in Croatia are rare and very rare species., Porodica Phyrganeidae broji u Europi 7 rodova i 20 vrsta. Iz ove porodice na području Hrvatske utvrđeno je 6 vrsta, od kojih su DNA barkodirane četiri vrste, zabilježene i prikupljene u posljednjih pet godina. Faunistički najzanimljiviji je nalaz vrste Trichostegia minor Curtis, 1834 s područja rijeke Gacke (regija Lika), gorska Hrvatska. U BOLD bazu upisane su četiri vrste sa šest DNA barkodiranih primjeraka. S područja rijeke Gacke je DNA barkodirano i upisano u BOLD bazu 10 primjeraka s osam vrsta, uključujući i vrstu T. minor. Filogenetska istraživanja temeljena na analizi DNA barkod podataka pokazala su zanimljive rezultate. Primjerak vrste T. minor prikupljen na području rijeke Gacke grupira se s primjerkom te vrste iz Austrije u zasebnu podrgrupu, što ukazuje na manje molekularne specifičnosti. Filogenetska analiza potvrdila je i opravdanost statusa podvrste iz Mongolije, Phryganea grandis rotundata Ulmer, 1905. U budućim istraživanjima faune Trichoptera Hrvatske nastavit će se istraživanja porodice Phyrganeidae u različitim dijelovima Hrvatske u svrhu utvrđivanja detaljnijeg rasprostranjenja svih zabilježenih vrsta, potencijalnog nalaza neke još nezabilježene vrste, ali i nalaza te DNA barkodiranja vrsta Hagenella clathrata (Kolenati, 1848) i Oligostomis reticulata (Linnaeus, 1761). Za te vrste bit će potrebno utvrditi i određene značajke u području konzervacijske biologije potrebne za njihovu zaštitu i zaštitu lokaliteta na kojima žive, jer je očito da su to vrlo rijetke vrste u fauni Hrvatske, a u koje pripada i vrsta T. minor; naime, 50% zabilježenih vrsta porodice Phyrganeidae u Hrvatskoj su rijetke i vrlo rijetke vrste.

Presence of mollusk assemblages was studied within red coralligenous algae Corallina officinalis L. along the southern Istrian coast. C. officinalis turfs can be considered a biodiversity reservoir, as they shelter numerous invertebrate species. The aim of this study was to identify mollusk species within these settlements using DNA barcoding as a method for detailed identification of mollusks. Nine locations and 18 localities with algal coverage range above 90% were chosen at four research areas. From 54 collected samples of C. officinalis turfs, a total of 46 mollusk species were identified. Molecular methods helped identify 16 gastropod, 14 bivalve and one polyplacophoran species. COI sequences for two bivalve species (Musculus cf. costulatus (Risso, 1826) and Gregariella semigranata (Reeve, 1858)) and seven gastropod species (Megastomia winfriedi Peñas &amp, Rolán, 1999, Eatonina sp. Thiele, 1912, Eatonina cossurae (Calcara, 1841), Crisilla cf. maculata (Monterosato, 1869), Alvania cf. carinata (da Costa, 1778), Vitreolina antiflexa (Monterosato, 1884) and Odostomia plicata (Montagu, 1803)) represent new BINs in BOLD database. This study contributes to new findings related to the high biodiversity of mollusks associated with widespread C. officinalis settlements along the southern coastal area of Istria.

The influences of river regulations on aquatic biota are insufficiently investigated. We assessed the level of ecological disturbance of the mayfly assemblages through the damming and flow alterations at two highly regulated Mediterranean rivers in Croatia: the Cetina River and its tributary, the Ruda. Contrary to our hypotheses, species richness, abundance and diversity of mayflies increased along the river’s course. At the sites directly influenced by the river regulations, mayfly assemblage changed: it was less diverse and less abundant. However, no obvious influence of the river regulation on the mayfly assemblages was detected for the sites that were indirectly influenced by these alterations. This was most likely due to specific karst hydrology, i.e., the presence of lateral springs along the course of the river. Multidimensional scaling analysis (MDS) revealed grouping of sites according to the similarity in physico-chemical water properties. The pH and water temperature were the most important environmental variables influencing mayfly assemblages. The data on ecology and distribution of mayflies presented in the current study represent a valuable background for further research and conservation practices in the Mediterranean region.

The biological control agent and alien invasive ladybird Harmonia axyridis (Pallas, 1773), once introduced for pest control in Europe, is now spreading very rapidly over the European continent, especially since 2002. In this paper we give the first documented record of H. axyridis from Albania, southeastern Europe. The species was recorded in three localities in Albania.

The caddisfly species Beraea dira McLachlan 1875 was recorded for the first time in Croatia in the upper course of the Bistrac River in October 2014.