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Your search keyword '"Miyamoto, Takaaki"' showing total 101 results
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101 results on '"Miyamoto, Takaaki"'

1. Design of an Artificial Peptide Inspired by Transmembrane Mitochondrial Protein for Escorting Exogenous DNA into the Mitochondria to Restore their Functions by Simultaneous Multiple Gene Expression (Adv. Funct. Mater. 8/2024)

Author

Yoshinaga, Naoto, primary, Miyamoto, Takaaki, additional, Odahara, Masaki, additional, Takeda‐Kamiya, Noriko, additional, Toyooka, Kiminori, additional, Nara, Seia, additional, Nishimura, Haruna, additional, Ling, Feng, additional, Su'etsugu, Masayuki, additional, Yoshida, Minoru, additional, and Numata, Keiji, additional

Published
2024
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2. Synthesis of Polypeptides

Author

Tsuchiya, Kousuke, Miyagi, Yu, Miyamoto, Takaaki, Gudeangadi, Prashant G., Numata, Keiji, He, Liang-Nian, Series Editor, Rogers, Robin D., Series Editor, Su, Dangsheng, Series Editor, Tundo, Pietro, Series Editor, Zhang, Z. Conrad, Series Editor, Kobayashi, Shiro, editor, Uyama, Hiroshi, editor, and Kadokawa, Jun-ichi, editor

Published
2019
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3. Design of an Artificial Peptide Inspired by Transmembrane Mitochondrial Protein for Escorting Exogenous DNA into the Mitochondria to Restore their Functions by Simultaneous Multiple Gene Expression

Author

Yoshinaga, Naoto, primary, Miyamoto, Takaaki, additional, Odahara, Masaki, additional, Takeda‐Kamiya, Noriko, additional, Toyooka, Kiminori, additional, Nara, Seia, additional, Nishimura, Haruna, additional, Ling, Feng, additional, Su'etsugu, Masayuki, additional, Yoshida, Minoru, additional, and Numata, Keiji, additional

Published
2023
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19. Phenylboronic Acid-Functionalized Micelles Dual-Targeting Boronic Acid Transporter and Polysaccharides for siRNA Delivery into Brown Algae

Author

Yoshinaga, Naoto, Miyamoto, Takaaki, Goto, Mami, Tanaka, Atsuko, and Numata, Keiji

Abstract

Brown algae play essential roles ecologically, practically, and evolutionarily because they maintain coastal areas, capture carbon dioxide, and produce valuable chemicals such as therapeutic drugs. To unlock their full potential, understanding the unique molecular biology of brown algae is imperative. Genetic engineering tools that regulate homeostasis in brown algae are essential for determining their biological mechanisms in detail. However, few methodologies have been developed to control gene expression due to the robust structural barriers of brown algae. To address this issue, we designed peptide-based, small interfering RNA (siRNA)-loaded micelles decorated with phenylboronic acid (PBA) ligands. The PBA ligands facilitated the cellular uptake of the micelles into a model brown alga, Ectocarpus siliculosus(E. Siliculosus), through chemical interaction with polysaccharides in the cell wall and biological recognition by boronic acid transporters on the plasma membrane. The micelles, featuring “kill two birds with one stone” ligands, effectively induced gene silencing related to auxin biosynthesis. As a result, the growth of E. siliculosuswas temporarily inhibited without persistent genome editing. This study demonstrated the potential for exploring the characteristics of brown algae through a simple yet effective approach and presented a feasible system for delivering siRNA in brown algae.

Published
2024
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22. Block Copolymer/Plasmid DNA Micelles Postmodified with Functional Peptides via Thiol–Maleimide Conjugation for Efficient Gene Delivery into Plants

Author

Miyamoto, Takaaki, Tsuchiya, Kousuke, and Numata, Keiji

Abstract

Introducing exogenous genes into plant cells is essential for a wide range of applications in agriculture and plant biotechnology fields. Cationic peptide carriers with cell-penetrating and DNA-binding domains successfully deliver exogenous genes into plants. However, their cell-penetrating activity may be attenuated by undesired electrostatic interactions between the cell-penetrating peptide (CPP) domain and DNA cargo, resulting in limited gene delivery efficiency. Here, we developed the block copolymer maleimide-conjugated tetra(ethylene glycol) and poly(l-lysine) (MAL-TEG-PLL). Through electrostatic interactions with plasmid DNA (pDNA), MAL-TEG-PLL formed a micelle that presented maleimide groups on its surface. The micelle enabled postmodification with cysteine-containing functional peptides, including a CPP (BP100-Cys) and nuclear localization signal (Cys-NLS) via thiol–maleimide conjugation, thereby avoiding undesired interactions. According to a comparison of gene delivery efficiencies among the peptide-postmodified micelles, the amount of BP100-Cys on the micelle surface was key for efficient gene delivery. The BP100-postmodified micelle showed more efficient delivery compared with that of the BP100-premodified micelle. Thus, postmodification of polymeric micelles with functional peptides opens the door to designing highly efficient plant gene delivery systems.

Published
2019
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26. Enhanced stability of Cu2+–ATCUN complexes under physiologically relevant conditions by insertion of structurally bulky and hydrophobic amino acid residues into the ATCUN motif.

Author

Miyamoto, Takaaki, Fukino, Yuta, Kamino, Shinichiro, Ueda, Masashi, and Enomoto, Shuichi

Subjects
*COPPER compounds, *NICKEL compounds, *AMINO acids, *HYDROPHOBIC compounds, *AQUEOUS solutions
Abstract

Copper complexes formed by an amino terminal Cu2+- and Ni2+-binding (ATCUN) motif have attracted attention as metallodrug candidates that cleave DNA or RNA and inactivate enzymes. Although the stability of the Cu2+–ATCUN complex under physiologically relevant conditions is a key factor for medical applications, it has remained unclear. Here we prepared a series of ATCUN peptides by inserting various amino acid residues into positions 1 and 2, and investigated the stability of the Cu2+–ATCUN complexes in aqueous solution, blood plasma, and living animals. Systematic pH titration showed that the low basicity of the N-terminal amine of the peptide stabilized the Cu2+–ATCUN complex in aqueous solution. Interestingly, the stability of 64Cu-labeled ATCUN complexes in blood plasma was significantly enhanced by the structural bulkiness and hydrophobicity of the amino acid residues at positions 1 and 2. To validate the in vivo stability, six ATCUN motifs (YYH, VVH, NNH, TTH, GGH, and DDH) were conjugated to a tumor-targeting peptide, octreotide (Oct). The stability of the 64Cu–ATCUN–Oct complexes in blood plasma showed a similar trend to that of the 64Cu–ATCUN complexes. The 64Cu–YYH–Oct complex exhibited the highest stability in blood plasma. According to the positron emission tomography and competitive blocking studies of a tumor-bearing mouse model, 64Cu–YYH–Oct specifically accumulated in tumors, suggesting that the complex was sufficiently stable to reach its target in vivo. The results show that the structural bulkiness and hydrophobicity of the residues at positions 1 and 2 are key parameters for designing metallodrugs on the basis of the Cu2+–ATCUN complex. [ABSTRACT FROM AUTHOR]

Published
2016
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43. Photocatalytic partial oxidation of α-methylstyrene over TiO2 supported on zeolites

Author

Yahiro, Hidenori, Miyamoto, Takaaki, Watanabe, Nobuyoshi, and Yamaura, Hiroyuki

Subjects
*PHOTOCATALYSIS, *OXIDATION, *ZEOLITES, *TITANIUM dioxide
Abstract

Abstract: The photocatalytic reaction in the partial oxidation of α-methylstyrene (α-MS) to acetophenone (AP) was investigated for TiO2 supported on Y-type, moredenite, and ZSM-5 zeolites. XRD and FT-Raman results demonstrated that the anatase is the stable phase of TiO2 supported on zeolites and no rutile phase was observed in the supported catalysts calcined at 723–973K. TiO2 supported on zeolites was found to catalyze the partial oxidation of α-MS to AP under light irradiation (>290nm). The photocatalytic activity of TiO2 supported on zeolites was significantly improved by the addition of the water to the organic solvent. It was found that the optimized amount of water added for TiO2 supported on zeolite catalysts was smaller than that for TiO2 catalyst. [Copyright &y& Elsevier]

Published
2007
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49. In situ stress measurement of chemical vapor deposited tungsten silicides.

Author

Washidzu, Gen, Hara, Tohru, Miyamoto, Takaaki, and Inoue, Toshihiro

Subjects
SILICIDES, TUNGSTEN compounds, CHEMICAL vapor deposition
Abstract

Describes the in situ stress measurement of chemical vapor deposited tungsten silicides. Problem of peeling in chemical vapor deposited tungsten silicide; Appearance of abrupt stress change in monosilane WSi[sub x].

Published
1991
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