12 results on '"Mitsuo Nishimoto"'
Search Results
2. Retinoic Acid Receptor β: A Potential Therapeutic Target in Retinoic Acid Treatment of Endometrial Cancer
- Author
-
Misaki Fue, Mayu Hanihara, Takashi Suzuki, Kiyoshi Takagi, Fumihiko Suzuki, Mitsuo Nishimoto, Kiyoshi Ito, Keita Tsuji, Yasuhiro Miki, Nobuo Yaegashi, and Hiroki Utsunomiya
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Cell Survival ,Receptors, Retinoic Acid ,Retinoic acid ,Tretinoin ,Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Movement ,Cell Line, Tumor ,Internal medicine ,medicine ,Humans ,Molecular Targeted Therapy ,Receptor ,Gene knockdown ,Cell growth ,Retinoic Acid Receptor alpha ,Obstetrics and Gynecology ,Immunohistochemistry ,Endometrial Neoplasms ,Reverse transcription polymerase chain reaction ,Retinoic acid receptor ,030104 developmental biology ,Endocrinology ,Oncology ,chemistry ,Cell culture ,Apoptosis ,Gene Knockdown Techniques ,030220 oncology & carcinogenesis ,Cancer research ,Female - Abstract
ObjectiveSeveral studies have reported that retinoic acid (RA) might be used to treat malignancies. The effects of RA are mediated by the RA receptor (RAR), and RARα/RARβ especially acts as a tumor suppressor. However, little is known about its role in human endometrial cancer.Materials and MethodsIn this study, we examined the effects of all-trans RA (ATRA) on progression of human endometrial cancer cell line, RL95-2 and Hec1A. We then examined the expression of RARα and RARβ in 50 endometrial cancer tissues by using immunohistochemistry.ResultsWe found inhibitory effects of ATRA on cell proliferation, apoptosis, and migration in RL95-2 cells, but not in Hec1A cells. RARα or RARβ knockdown individually could not cancel out the inhibition of cell proliferation by ATRA in RL95-2 cells, but simultaneous knockdown of RARα and RARβ could block its effect on proliferation. RARα and RARβ knockdown dose dependently reduced the inhibition of migration by ATRA, but the effect was more pronounced with RARβ knockdown than with RARα knockdown. We confirmed that RARβ gene was directly regulated by ATRA in microarray and real-time reverse transcription polymerase chain reaction. Furthermore, the RARβ agonist (BMS453) significantly suppressed proliferation of RL95-2 cells. In immunohistochemical analysis, RARα expression was positively correlated with tumor grade, and RARβ showed the opposite tendency in endometrial cancer.ConclusionsRetinoic acid might have multiple antitumor effects, and RARβ may be a potent therapeutic target in RA treatment for endometrial cancers.
- Published
- 2017
3. Development of a new clinically applicable device for embryo evaluation which measures embryo oxygen consumption
- Author
-
Hideki Igarashi, Hiroyuki Abe, Toshifumi Takahashi, Masahito Tachibana, Jin Kumagai, Hiroki Kurosawa, Motomasa Ihara, Ryota Suganuma, Aiko Takahashi, Nobuo Yaegashi, Masumi Ishibashi, Hiroki Utsunomiya, Yukihiro Terada, Mitsuo Nishimoto, Naomi Shiga, Atsushi Fukui, and Zen Watanabe
- Subjects
0301 basic medicine ,Embryonic Development ,chemistry.chemical_element ,Single Embryo Transfer ,Fertilization in Vitro ,Biology ,Oxygen ,Embryo Culture Techniques ,Toxicology ,Andrology ,03 medical and health sciences ,Oxygen Consumption ,0302 clinical medicine ,Pregnancy ,medicine ,Animals ,Humans ,Blastocyst ,030219 obstetrics & reproductive medicine ,Rehabilitation ,Obstetrics and Gynecology ,Embryo ,Embryo culture ,Embryo Transfer ,Embryo transfer ,030104 developmental biology ,medicine.anatomical_structure ,Reproductive Medicine ,chemistry ,embryonic structures ,Cattle ,Female ,Limiting oxygen concentration ,Embryo quality - Abstract
Study question Does a new system-the chip-sensing embryo respiration monitoring system (CERMs)-enable evaluation of embryo viability for potential application in a clinical IVF setting? Summary answer The system enabled the oxygen consumption rate of spheroids, bovine embryos and frozen-thawed human embryos to be measured, and this rate corresponded to the developmental potential of embryos. What is already known To date, no reliable and clinically suitable objective evaluation methods for embryos are available, which circumvent the differences in inter-observer subjective view. Existing systems such as the scanning electrochemical microscopy (SECM) technique, which enables the measurement of oxygen consumption rate in embryos, need improvement in usability before they can be applied to a clinical setting. Study design, size, duration This is a prospective original research study. The feasibility of measuring the oxygen consumption rate was assessed using CERMs for 9 spheroids, 9 bovine embryos and 30 redundant frozen-thawed human embryos. The endpoints for the study were whether CERMs could detect a dissolved oxygen gradient with high sensitivity, had comparable accuracy to the SECM measuring system with improved usability, and could predict the development of an embryo to a blastocyst by measuring the oxygen consumption rate. The relationship between the oxygen consumption rate and standard morphological evaluation was also examined. Participants/materials, setting, methods We developed a new CERMs, which enables the oxygen consumption rate to be measured automatically using an electrochemical method. The device was initially used for measuring a dissolved oxygen concentration gradient in order to calculate oxygen consumption rate using nine spheroids. Next, we evaluated data correlation between the CERMs and the SECM measuring systems using nine bovine embryos. Finally, the oxygen consumption rates of 30 human embryos, which were frozen-thawed on 2nd day after fertilization, were measured by CERMs at 6, 24, 48, 72 and 96 h after thawing with standard morphological evaluation. Furthermore, the developed blastocysts were scored using the blastocyst quality score (BQS), and the correlation with oxygen consumption rate was also assessed. Main results and the role of chance The device enabled the oxygen consumption rate of an embryo to be measured automatically within a minute. The oxygen concentration gradient profile showed excellent linearity in a distance-dependent change. A close correlation in the oxygen consumption rates of bovine embryos was observed between the SECM measuring system and CERMs, with a determination coefficient of 0.8203 (P = 0.0008). Oxygen consumption rates of human embryos that have reached the blastocyst stage were significantly higher than those of arrested embryos at 48, 72 and 96 h after thawing (P = 0.039, 0.004 and 0.049, respectively). Thus, in vitro development of frozen-thawed human embryos to the blastocyst stage would be predicted at 48 h after thawing (day 4) by measuring the oxygen consumption using CERMs. Although a positive linear relationship between BQS and the oxygen consumption rate was observed [the determination coefficient was R(2) = 0.6537 (P = 0.008)], two blastocysts exhibited low oxygen consumption rates considering their relatively high BQS. This suggests that morphology and metabolism in human embryos might not correlate consistently. Limitations, reasons for caution Transfer of the embryo and pregnancy evaluation was not performed. Thus, a correlation between oxygen consumption and the in vivo viability of embryos remains unknown. Clinical trials, including embryo transfer, would be desirable to determine a threshold value to elect clinically relevant, quality embryos for transfer. We utilized frozen-thawed human embryos in this study. The effect of these manipulations on the respiratory activity of the embryo is also unknown. Wider implications of the findings Selection of quality embryos, especially in a single embryo transfer cycle, by CERMs may have an impact on obtaining better clinical outcomes, albeit with clinical trials being required. Furthermore, the early determination of quality embryos by CERMs may enable the omission of long-term in vitro embryo culture to the blastocyst stage. CERMs is scalable technology that can be integrated into incubators and/or other embryo evaluation systems, such as the time-lapse systems, due to its chip-based architecture. Thus, CERMS would enable automatic measurement of oxygen consumption, under 5% CO2, in the near future, in order to reduce oxidative stress from exposure to atmospheric air. Study funding/competing interests This study was supported by grants from the Health and Labor Sciences Research Grant (H24-Hisaichiiki-Shitei-016). The authors have no conflicts of interest. Trial registration number Not applicable.
- Published
- 2016
4. Steroid Sulfatase Inhibitor Reduces Proliferation of Ishikawa Endometrial Cancer Cells in Co-Culture Systems
- Author
-
Hironobu Sasano, Hidekazu Nishigori, Satoru Nagase, Hiroki Utsunomiya, Mitsuo Nishimoto, Takashi Suzuki, Masafumi Toyoshima, Nobuo Yaegashi, Naomi Shiga, Fumihiko Suzuki, and Kiyoshi Ito
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Aromatase inhibitor ,biology ,medicine.drug_class ,Androstenediol ,Estrogen receptor ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,0302 clinical medicine ,Endocrinology ,chemistry ,Estrone sulfate ,Estradiol sulfate ,Estrogen ,030220 oncology & carcinogenesis ,Internal medicine ,biology.protein ,medicine ,Steroid sulfatase ,Aromatase - Abstract
Objectives: Estrogens significantly contribute toward the growth and development of endometrial cancers. Two principal pathways have been implicated in the final steps of estrogen synthesis: the steroid sulfatase (STS) and aromatase pathways. In this study, we aimed to evaluate the possible effects of tumor-stromal interactions on local estrogen biosynthesis in endometrial cancer. We also assessed the biological effects of inhibitors of steroid sulfatase and aromatase in the co-culture system compared with usual monocultures. Methods/Materials: We isolated stromal cells from endometrial cancer patients to examine local biosynthesis of estrogens and tumor-stromal interactions. Next we examined the effects of steroid sulfatase inhibitor and aromatase inhibitor in monoculture of endometrial cancer cell line (Ishikawa) and in a co-culture system involving an Ishikawa cells and stromal cells. Results: Estrogen receptor and steroid sulfatase mRNA levels in cancer cells were significantly higher in the co-cultures compared with the monocultures of endometrial cancer cells. Estradiol and androstenediol concentrations were also significantly higher in the co-cultured cells. Proliferation of the cancer cells was significantly increased through the steroid sulfatase pathway, which metabolizes androgens, estrone sulfate, and estradiol sulfate as its substrates. However, its proliferation was significantly decreased by the treatment of steroid sulfatase or aromatase inhibitors. The significant growth inhibition by the steroid sulfatase and aromatase inhibitors were also observed in the co-culture system. Conclusions: We evaluated the effects of STS inhibitor and aromatase inhibitors on the proliferation of estrogen-dependent endometrial cancer cells. Considering that intratumoral estrogen metabolism plays an important role, our co-culture systems provide an environment similar to that of the tumor in living patients in terms of metabolism and synthesis of intratumoral estrogens. The results of this study may aid in achieving improved clinical responses from patients treated with STS inhibitors.
- Published
- 2016
5. A Case of Quiescent Nuck's Hydrocele with Endometriosis Revealed after Laparoscopic Surgery
- Author
-
Nobuo Yaegashi, Hiroki Kurosawa, Masumi Ishibashi, Zen Watanabe, Tadashi Watanabe, Masafumi Toyoshima, Hiroshi Nabeshima, Naomi Shiga, Mitsuo Nishimoto, and Hiroki Utsunomiya
- Subjects
Laparoscopic surgery ,medicine.medical_specialty ,business.industry ,medicine.medical_treatment ,General surgery ,Hydrocele ,Endometriosis ,medicine ,medicine.disease ,Complication ,business ,Surgery - Published
- 2013
6. Laparoscopic Strassman metroplasty in a postmenarcheal adolescent girl with Herlyn-Werner-Wunderlich müllerian anomaly variant, obstructed noncommunicating didelphic uterus without gartner duct pseudocyst
- Author
-
Naomi Shiga, Mitsuo Nishimoto, Hiroki Utsunomiya, Nobuo Yaegashi, and Hiroshi Nabeshima
- Subjects
medicine.medical_specialty ,Bicornuate uterus ,Metroplasty ,Mullerian Ducts ,Uterus ,Dysmenorrhea ,Medicine ,Humans ,Abnormalities, Multiple ,Laparoscopy ,Child ,Gynecology ,Hematometra ,Menarche ,medicine.diagnostic_test ,business.industry ,Obstetrics and Gynecology ,medicine.disease ,Uterus didelphys ,Surgery ,medicine.anatomical_structure ,Female ,business ,Duct (anatomy) - Abstract
Asymmetric obstructed uterus didelphys (Herlyn-Werner-Wunderlich syndrome), also known as obstructed hemivagina with ipsilateral renal agenesis syndrome, is a rare congenital mullerian duct anomaly. Herein we present a case report of incomplete Herlyn-Werner-Wunderlich syndrome, with absence of the hemivaginal septum, diagnosed in a 12-year-old girl. Treatment of the severe pain using an analgesic agent was ineffective. Therefore, laparoscopic metroplastic surgery via the modified Strassman procedure was performed. After surgery, the patient no longer reported dysmenorrhea.
- Published
- 2012
7. Total Laparoscopic Conservative Surgery for an Intramural Ectopic Pregnancy
- Author
-
Tomohisa Ugajin, Mitsuo Nishimoto, Hiroki Utsunomiya, Nobuo Yaegashi, Hiroshi Nabeshima, Yukihiro Terada, and Maiko Arai
- Subjects
Gynecology ,medicine.medical_specialty ,Hysterectomy ,lcsh:Medical technology ,medicine.diagnostic_test ,Ectopic pregnancy ,business.industry ,medicine.medical_treatment ,Gestational sac ,Cornual Pregnancy ,Uterus ,Myometrium ,Case Report ,medicine.disease ,Surgery ,medicine.anatomical_structure ,lcsh:R855-855.5 ,medicine ,Chorionic villi ,Radiology, Nuclear Medicine and imaging ,Laparoscopy ,business - Abstract
A 38-year-old woman, gravida 3, para 1 with a history of a left salpingectomy for an ectopic pregnancy was admitted for treatment of a presumed ectopic pregnancy. Transvaginal sonography revealed an ill-defined gestational sac and fetal heart beat within the fundal myometrium adjacent to the left cornua. Laparoscopy was performed for a suspected left cornual pregnancy or intramural pregnancy. A cystic mass 3 cm in diameter was visible within the fundal myometrium. Total laparoscopic removal of the gestational sac was performed, and the uterus was preserved. Pathologic evaluation of the excised mass demonstrated chorionic villi involving the myometrium. In the literature, only one other case describing the laparoscopic removal of an intramural pregnancy has been reported. However, in the prior report, the patient still required hysterectomy after conservative surgery. Therefore, this is the first report of the successful treatment of an intramural pregnancy exclusively with laparoscopy.
- Published
- 2010
8. Successful total laparoscopic cystic adenomyomectomy after unsuccessful open surgery using transtrocar ultrasonographic guiding
- Author
-
Takashi Murakami, Naoko Sato, Noboru Sugawara, Hiroshi Nabeshima, and Mitsuo Nishimoto
- Subjects
Adult ,medicine.medical_specialty ,medicine.medical_treatment ,Signs and symptoms ,Intraoperative Period ,Dysmenorrhea ,Laparotomy ,medicine ,Humans ,Cyst ,Laparoscopic resection ,Treatment Failure ,Laparoscopy ,Adenomyoma ,Ultrasonography ,medicine.diagnostic_test ,business.industry ,Open surgery ,General surgery ,Obstetrics and Gynecology ,medicine.disease ,Surgery ,Uterine Neoplasms ,Disease Progression ,Female ,business - Abstract
A 27-year-old woman with a cystic adenomyoma located within the myometrium underwent laparotomy unsuccessfully, with persistent postoperative heavy dysmenorrhea. Total laparoscopic resection of the cystic adenomyoma was then attempted. Intraoperative transtrocar ultrasonography was used to detect the location and boundaries of the cystic adenomyoma. The cyst was removed laparoscopically, and dysmenorrhea completely disappeared postoperatively. This is the first report of total laparoscopic resection of cystic adenomyoma after unsuccessful laparotomy, a minimally invasive approach that successfully eliminated the patient's severe signs and symptoms.
- Published
- 2007
9. Retinoic Acid Receptor β: A Potential Therapeutic Target in Retinoic Acid Treatment of Endometrial Cancer.
- Author
-
Keita Tsuji, Hiroki Utsunomiya, Yasuhiro Miki, Mayu Hanihara, Misaki Fue, Kiyoshi Takagi, Mitsuo Nishimoto, Fumihiko Suzuki, Nobuo Yaegashi, Takashi Suzuki, and Kiyoshi Ito
- Abstract
Objective: Several studies have reported that retinoic acid (RA) might be used to treat malignancies. The effects of RA are mediated by the RA receptor (RAR), and RARα/RARβ especially acts as a tumor suppressor. However, little is known about its role in human endometrial cancer. Materials and Methods: In this study, we examined the effects of all-trans RA (ATRA) on progression of human endometrial cancer cell line, RL95-2 and Hec1A. We then examined the expression ofRARα andRARβ in 50 endometrial cancer tissues by usingimmunohistochemistry. Results: We found inhibitory effects ofATRAon cell proliferation, apoptosis, and migration in RL95-2 cells, but not in Hec1A cells. RARα or RARβ knockdown individually could not cancel out the inhibition of cell proliferation by ATRA in RL95-2 cells, but simultaneous knockdown of RAR> and RARA could block its effect on proliferation. RARα and RARβ knockdown dose dependently reduced the inhibition of migration by ATRA, but the effect was more pronounced with RARA knockdown than with RARα knockdown. We confirmed that RARA gene was directly regulated by ATRA in microarray and real-time reverse transcription polymerase chain reaction. Furthermore, theRARβ agonist (BMS453) significantly suppressed proliferation of RL95-2 cells. In immunohistochemical analysis, RAR> expression was positively correlated with tumor grade, and RARβ showed the opposite tendency in endometrial cancer. Conclusions: Retinoic acid might have multiple antitumor effects, and RARβ may be a potent therapeutic target in RA treatment for endometrial cancers. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
10. Echo-Laparoscopy for Cystic Adenomyoma: Three Cases Report of Total Laparoscopic Management
- Author
-
N. Kashiwadate, Takashi Murakami, H. Nabeshima, Mitsuo Nishimoto, and N. Sugawara
- Subjects
medicine.medical_specialty ,medicine.diagnostic_test ,business.industry ,Echo (computing) ,medicine ,Obstetrics and Gynecology ,Radiology ,business ,medicine.disease ,Laparoscopy ,Adenomyoma - Published
- 2008
11. Development of a new clinically applicable device for embryo evaluation which measures embryo oxygen consumption.
- Author
-
Hiroki Kurosawa, Hiroki Utsunomiya, Naomi Shiga, Aiko Takahashi, Motomasa Ihara, Masumi Ishibashi, Mitsuo Nishimoto, Zen Watanabe, Hiroyuki Abe, Jin Kumagai, Yukihiro Terada, Hideki Igarashi, Toshifumi Takahashi, Atsushi Fukui, Ryota Suganuma, Masahito Tachibana, Nobuo Yaegashi, Kurosawa, Hiroki, Utsunomiya, Hiroki, and Shiga, Naomi
- Subjects
OXYGEN consumption ,MEDICAL innovations ,EMBRYOLOGY ,FERTILIZATION in vitro ,SCANNING electrochemical microscopy ,BLASTOCYST ,ANIMALS ,CATTLE ,EMBRYO transfer ,FETAL development ,PHYSIOLOGY - Abstract
Study Question: Does a new system-the chip-sensing embryo respiration monitoring system (CERMs)-enable evaluation of embryo viability for potential application in a clinical IVF setting?Summary Answer: The system enabled the oxygen consumption rate of spheroids, bovine embryos and frozen-thawed human embryos to be measured, and this rate corresponded to the developmental potential of embryos.What Is Already Known: To date, no reliable and clinically suitable objective evaluation methods for embryos are available, which circumvent the differences in inter-observer subjective view. Existing systems such as the scanning electrochemical microscopy (SECM) technique, which enables the measurement of oxygen consumption rate in embryos, need improvement in usability before they can be applied to a clinical setting.Study Design, Size, Duration: This is a prospective original research study. The feasibility of measuring the oxygen consumption rate was assessed using CERMs for 9 spheroids, 9 bovine embryos and 30 redundant frozen-thawed human embryos. The endpoints for the study were whether CERMs could detect a dissolved oxygen gradient with high sensitivity, had comparable accuracy to the SECM measuring system with improved usability, and could predict the development of an embryo to a blastocyst by measuring the oxygen consumption rate. The relationship between the oxygen consumption rate and standard morphological evaluation was also examined.Participants/materials, Setting, Methods: We developed a new CERMs, which enables the oxygen consumption rate to be measured automatically using an electrochemical method. The device was initially used for measuring a dissolved oxygen concentration gradient in order to calculate oxygen consumption rate using nine spheroids. Next, we evaluated data correlation between the CERMs and the SECM measuring systems using nine bovine embryos. Finally, the oxygen consumption rates of 30 human embryos, which were frozen-thawed on 2nd day after fertilization, were measured by CERMs at 6, 24, 48, 72 and 96 h after thawing with standard morphological evaluation. Furthermore, the developed blastocysts were scored using the blastocyst quality score (BQS), and the correlation with oxygen consumption rate was also assessed.Main Results and the Role Of Chance: The device enabled the oxygen consumption rate of an embryo to be measured automatically within a minute. The oxygen concentration gradient profile showed excellent linearity in a distance-dependent change. A close correlation in the oxygen consumption rates of bovine embryos was observed between the SECM measuring system and CERMs, with a determination coefficient of 0.8203 (P = 0.0008). Oxygen consumption rates of human embryos that have reached the blastocyst stage were significantly higher than those of arrested embryos at 48, 72 and 96 h after thawing (P = 0.039, 0.004 and 0.049, respectively). Thus, in vitro development of frozen-thawed human embryos to the blastocyst stage would be predicted at 48 h after thawing (day 4) by measuring the oxygen consumption using CERMs. Although a positive linear relationship between BQS and the oxygen consumption rate was observed [the determination coefficient was R(2) = 0.6537 (P = 0.008)], two blastocysts exhibited low oxygen consumption rates considering their relatively high BQS. This suggests that morphology and metabolism in human embryos might not correlate consistently.Limitations, Reasons For Caution: Transfer of the embryo and pregnancy evaluation was not performed. Thus, a correlation between oxygen consumption and the in vivo viability of embryos remains unknown. Clinical trials, including embryo transfer, would be desirable to determine a threshold value to elect clinically relevant, quality embryos for transfer. We utilized frozen-thawed human embryos in this study. The effect of these manipulations on the respiratory activity of the embryo is also unknown.Wider Implications Of the Findings: Selection of quality embryos, especially in a single embryo transfer cycle, by CERMs may have an impact on obtaining better clinical outcomes, albeit with clinical trials being required. Furthermore, the early determination of quality embryos by CERMs may enable the omission of long-term in vitro embryo culture to the blastocyst stage. CERMs is scalable technology that can be integrated into incubators and/or other embryo evaluation systems, such as the time-lapse systems, due to its chip-based architecture. Thus, CERMS would enable automatic measurement of oxygen consumption, under 5% CO2, in the near future, in order to reduce oxidative stress from exposure to atmospheric air.Study Funding/competing Interests: This study was supported by grants from the Health and Labor Sciences Research Grant (H24-Hisaichiiki-Shitei-016). The authors have no conflicts of interest.Trial Registration Number: Not applicable. [ABSTRACT FROM AUTHOR]- Published
- 2016
- Full Text
- View/download PDF
12. Characteristics and applications of sponge metal
- Author
-
Mitsuo Nishimoto
- Subjects
Metal ,Sponge ,Materials science ,biology ,Chemical engineering ,visual_art ,visual_art.visual_art_medium ,biology.organism_classification - Published
- 1989
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