14 results on '"Missaglia, C"'
Search Results
2. A novel oncogenic BTK isoform is overexpressed in colon cancers and required for RAS-mediated transformation
- Author
-
Grassilli, E, Pisano, F, Cialdella, A, Bonomo, S, Missaglia, C, Cerrito, M G, Masiero, L, Ianzano, L, Giordano, F, Cicirelli, V, Narloch, R, D’Amato, F, Noli, B, Ferri, G L, Leone, B E, Stanta, G, Bonin, S, Helin, K, Giovannoni, R, and Lavitrano, M
- Published
- 2016
- Full Text
- View/download PDF
3. BTK inhibitors synergise with 5-FU to treat drug-resistant TP53-null colon cancers
- Author
-
Lavitrano, M, Ianzano, L, Bonomo, S, Cialdella, A, Cerrito, M, Pisano, F, Missaglia, C, Giovannoni, R, Romano, G, Mclean, C, Voest, E, D'Amato, F, Noli, B, Ferri, G, Agostini, M, Pucciarelli, S, Helin, K, Leone, B, Canzonieri, V, Grassilli, E, Lavitrano, Marialuisa, Ianzano, Leonarda, Bonomo, Sara, Cialdella, Annamaria, Cerrito, Maria Grazia, Pisano, Fabio, Missaglia, Carola, Giovannoni, Roberto, Romano, Gabriele, McLean, Chelsea M, Voest, Emile E, D'Amato, Filomena, NOLI, BARBARA, Ferri, Gian Luca, Agostini, Marco, Pucciarelli, Salvatore, Helin, Kristian, Leone, Biagio Eugenio, Canzonieri, Vincenzo, Grassilli, Emanuela, Lavitrano, M, Ianzano, L, Bonomo, S, Cialdella, A, Cerrito, M, Pisano, F, Missaglia, C, Giovannoni, R, Romano, G, Mclean, C, Voest, E, D'Amato, F, Noli, B, Ferri, G, Agostini, M, Pucciarelli, S, Helin, K, Leone, B, Canzonieri, V, Grassilli, E, Lavitrano, Marialuisa, Ianzano, Leonarda, Bonomo, Sara, Cialdella, Annamaria, Cerrito, Maria Grazia, Pisano, Fabio, Missaglia, Carola, Giovannoni, Roberto, Romano, Gabriele, McLean, Chelsea M, Voest, Emile E, D'Amato, Filomena, NOLI, BARBARA, Ferri, Gian Luca, Agostini, Marco, Pucciarelli, Salvatore, Helin, Kristian, Leone, Biagio Eugenio, Canzonieri, Vincenzo, and Grassilli, Emanuela
- Abstract
Colorectal cancer (CRC) is the fourth cause of death from cancer worldwide mainly due to the high incidence of drug-resistance. During a screen for new actionable targets in drug-resistant tumours we recently identified p65BTK – a novel oncogenic isoform of Bruton's tyrosine kinase. Studying three different cohorts of patients here we show that p65BTK expression correlates with histotype and cancer progression. Using drug-resistant TP53-null colon cancer cells as a model we demonstrated that p65BTK silencing or chemical inhibition overcame the 5-fluorouracil resistance of CRC cell lines and patient-derived organoids and significantly reduced the growth of xenografted tumours. Mechanistically, we show that blocking p65BTK in drug-resistant cells abolished a 5-FU-elicited TGFB1 protective response and triggered E2F-dependent apoptosis. Taken together, our data demonstrated that targeting p65BTK restores the apoptotic response to chemotherapy of drug-resistant CRCs and gives a proof-of-concept for suggesting the use of BTK inhibitors in combination with 5-FU as a novel therapeutic approach in CRC patients. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
- Published
- 2020
4. GSK3A Is Redundant with GSK3B in Modulating Drug Resistance and Chemotherapy-Induced Necroptosis
- Author
-
Grassilli, E, Ianzano, L, Bonomo, S, Missaglia, C, Cerrito, M, Giovannoni, R, Masiero, L, Lavitrano, M, GRASSILLI, EMANUELA, IANZANO, LEONARDA, BONOMO, SARA MARIA, CERRITO, MARIA GRAZIA, GIOVANNONI, ROBERTO, MASIERO, LAURA, LAVITRANO, MARIALUISA, Grassilli, E, Ianzano, L, Bonomo, S, Missaglia, C, Cerrito, M, Giovannoni, R, Masiero, L, Lavitrano, M, GRASSILLI, EMANUELA, IANZANO, LEONARDA, BONOMO, SARA MARIA, CERRITO, MARIA GRAZIA, GIOVANNONI, ROBERTO, MASIERO, LAURA, and LAVITRANO, MARIALUISA
- Abstract
Glycogen Synthase Kinase-3 alpha (GSK3A) and beta (GSK3B) isoforms are encoded by distinct genes, are 98% identical within their kinase domain and perform similar functions in several settings; however, they are not completely redundant and, depending on the cell type and differentiative status, they also play unique roles. We recently identified a role for GSK3B in drug resistance by demonstrating that its inhibition enables necroptosis in response to chemotherapy in p53-null drug-resistant colon carcinoma cells. We report here that, similarly to GSK3B, also GSK3A silencing/inhibition does not affect cell proliferation or cell cycle but only abolishes growth after treatment with DNA-damaging chemotherapy. In particular, blocking GSK3A impairs DNA repair upon exposure to DNA-damaging drugs. As a consequence, p53-null cells overcome their inability to undergo apoptosis and mount a necroptotic response, characterized by absence of caspase activation and RIP1-independent, PARP-dependent AIF nuclear re-localization. We therefore conclude that GSK3A is redundant with GSK3B in regulating drug-resistance and chemotherapy-induced necroptosis and suggest that inhibition of only one isoform, or rather partial inhibition of overall cellular GSK3 activity, is enough to re-sensitize drug-resistant cells to chemotherapy.
- Published
- 2014
5. BTK inhibitors synergise with 5-FU to treat drug-resistant TP53-null colon cancers
- Author
-
Fabio Pisano, Marialuisa Lavitrano, Gian-Luca Ferri, Filomena D'Amato, Emile E. Voest, Marco Agostini, Sara Bonomo, Annamaria Cialdella, Carola Missaglia, Gabriele Romano, Maria Grazia Cerrito, Kristian Helin, Roberto Giovannoni, Emanuela Grassilli, Vincenzo Canzonieri, Biagio Eugenio Leone, Barbara Noli, Leonarda Ianzano, Salvatore Pucciarelli, Chelsea M. McLean, Lavitrano, M, Ianzano, L, Bonomo, S, Cialdella, A, Cerrito, M, Pisano, F, Missaglia, C, Giovannoni, R, Romano, G, Mclean, C, Voest, E, D'Amato, F, Noli, B, Ferri, G, Agostini, M, Pucciarelli, S, Helin, K, Leone, B, Canzonieri, V, Grassilli, E, Lavitrano, Marialuisa, Ianzano, Leonarda, Bonomo, Sara, Cialdella, Annamaria, Cerrito, Maria Grazia, Pisano, Fabio, Missaglia, Carola, Giovannoni, Roberto, Romano, Gabriele, Mclean, Chelsea M, Voest, Emile E, D'Amato, Filomena, Noli, Barbara, Ferri, Gian Luca, Agostini, Marco, Pucciarelli, Salvatore, Helin, Kristian, Leone, Biagio E, Canzonieri, Vincenzo, and Grassilli, Emanuela
- Subjects
0301 basic medicine ,Colorectal cancer ,medicine.medical_treatment ,Mice, Nude ,Apoptosis ,Drug resistance ,Pathology and Forensic Medicine ,Transforming Growth Factor beta1 ,03 medical and health sciences ,0302 clinical medicine ,drug-resistance ,Antineoplastic Combined Chemotherapy Protocols ,Agammaglobulinaemia Tyrosine Kinase ,Tumor Cells, Cultured ,Gene silencing ,Medicine ,Animals ,Humans ,Protein Isoforms ,Molecular Targeted Therapy ,TP53 ,Protein Kinase Inhibitors ,Neoplasm Staging ,Chemotherapy ,business.industry ,BTK inhibitor ,Cancer ,Drug Synergism ,medicine.disease ,Genes, p53 ,Xenograft Model Antitumor Assays ,E2F Transcription Factors ,Organoids ,BTK inhibitors ,030104 developmental biology ,colon cancer ,Cell culture ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,Cancer research ,Disease Progression ,Fluorouracil ,p65BTK ,business ,Tyrosine kinase ,Colon cancer - Abstract
Colorectal cancer (CRC) is the fourth cause of death from cancer worldwide mainly due to the high incidence of drug-resistance. During a screen for new actionable targets in drug-resistant tumours we recently identified p65BTK–a novel oncogenic isoform of Bruton's tyrosine kinase. Studying three different cohorts of patients here we show that p65BTK expression correlates with histotype and cancer progression. Using drug-resistant TP53-null colon cancer cells as a model we demonstrated that p65BTK silencing or chemical inhibition overcame the 5-fluorouracil resistance of CRC cell lines and patient-derived organoids and significantly reduced the growth of xenografted tumours. Mechanistically, we show that blocking p65BTK in drug-resistant cells abolished a 5-FU-elicited TGFB1 protective response and triggered E2F-dependent apoptosis. Taken together, our data demonstrated that targeting p65BTK restores the apoptotic response to chemotherapy of drug-resistant CRCs and gives a proof-of-concept for suggesting the use of BTK inhibitors in combination with 5-FU as a novel therapeutic approach in CRC patients. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.
- Published
- 2020
6. A novel oncogenic BTK isoform is overexpressed in colon cancers and required for RAS-mediated transformation
- Author
-
Biagio Eugenio Leone, Roberto Giovannoni, Vittoria Cicirelli, Federica Giordano, Marialuisa Lavitrano, Fabio Pisano, Serena Bonin, Leonarda Ianzano, Annamaria Cialdella, Maria Grazia Cerrito, Emanuela Grassilli, Filomena D'Amato, Barbara Noli, Laura Masiero, Robert Narloch, Giorgio Stanta, Gian-Luca Ferri, Kristian Helin, Sara Bonomo, Carola Missaglia, Grassilli, E, Pisano, F, Cialdella, A, Bonomo, S, Missaglia, C, Cerrito, M, Masiero, L, Ianzano, L, Giordano, F, Cicirelli, V, Narloch, R, D’Amato, F, Noli, B, Ferri, G, Leone, B, Stanta, G, Bonin, S, Helin, K, Giovannoni, R, Lavitrano, M, Grassilli, Emanuela, Pisano, Fabio, Cialdella, Annamaria, Bonomo, Sara, Missaglia, Carola, Cerrito, Maria Grazia, Masiero, Laura, Ianzano, Leonarda, Giordano, Federica, Cicirelli, Vittoria, Narloch, Robert, D'Amato, Filomena, Noli, Barbara, Ferri, Gian Luca, Leone, Biagio, Stanta, Giorgio, Bonin, Serena, Helin, Kristian, Giovannoni, Roberto, and Lavitrano, Marialuisa
- Subjects
0301 basic medicine ,MAPK/ERK pathway ,Gene isoform ,Cancer Research ,MAP Kinase Signaling System ,Bruton's tyroine kinase ,Heterogeneous-Nuclear Ribonucleoprotein K ,03 medical and health sciences ,0302 clinical medicine ,Growth factor receptor ,oncogene ,Cell Line, Tumor ,Genetics ,Agammaglobulinaemia Tyrosine Kinase ,Bruton's tyrosine kinase ,Humans ,Protein Isoforms ,RAS-mediated transformation ,colon cancer ,RAS ,Protein kinase A ,Molecular Biology ,BTK ,isoform ,Oncogene ,biology ,Cell cycle ,Protein-Tyrosine Kinases ,Molecular biology ,030104 developmental biology ,Cell Transformation, Neoplastic ,030220 oncology & carcinogenesis ,Colonic Neoplasms ,biology.protein ,Cancer research ,ras Proteins ,colon cancer, oncogene, Bruton's tyroine kinase, RAS ,Original Article ,5' Untranslated Regions ,Tyrosine kinase - Abstract
Bruton's tyrosine kinase (BTK) is essential for B-cell proliferation/differentiation and it is generally believed that its expression and function are limited to bone marrow-derived cells. Here, we report the identification and characterization of p65BTK, a novel isoform abundantly expressed in colon carcinoma cell lines and tumour tissue samples. p65BTK protein is expressed, through heterogeneous nuclear ribonucleoprotein K (hnRNPK)-dependent and internal ribosome entry site-driven translation, from a transcript containing an alternative first exon in the 5'-untranslated region, and is post-transcriptionally regulated, via hnRNPK, by the mitogen-activated protein kinase (MAPK) pathway. p65BTK is endowed with strong transforming activity that depends on active signal-regulated protein kinases-1/2 (ERK1/2) and its inhibition abolishes RAS transforming activity. Accordingly, p65BTK overexpression in colon cancer tissues correlates with ERK1/2 activation. Moreover, p65BTK inhibition affects growth and survival of colon cancer cells. Our data reveal that BTK, via p65BTK expression, is a novel and powerful oncogene acting downstream of the RAS/MAPK pathway and suggest that its targeting may be a promising therapeutic approach.Oncogene advance online publication, 25 January 2016; doi:10.1038/onc.2015.504.
- Published
- 2016
7. GSK3A Is Redundant with GSK3B in Modulating Drug Resistance and Chemotherapy-Induced Necroptosis
- Author
-
Emanuela Grassilli, Leonarda Ianzano, Laura Masiero, Maria Grazia Cerrito, Sara Bonomo, Carola Missaglia, Marialuisa Lavitrano, Roberto Giovannoni, Grassilli, E, Ianzano, L, Bonomo, S, Missaglia, C, Cerrito, M, Giovannoni, R, Masiero, L, and Lavitrano, M
- Subjects
Cell type ,Programmed cell death ,DNA repair ,Necroptosis ,Cancer Treatment ,lcsh:Medicine ,Apoptosis ,colon carcinoma ,Biology ,chemotherapy ,GSK3 ,Glycogen Synthase Kinase 3 ,Necrosis ,Cell Signaling ,GSK-3 ,Cell Line, Tumor ,Molecular Cell Biology ,Gastrointestinal Tumors ,Anti-Apoptotic Signaling ,Medicine and Health Sciences ,Humans ,lcsh:Science ,GSK3B ,Apoptotic Signaling ,Glycogen Synthase Kinase 3 beta ,Multidisciplinary ,Cell Death ,Cell growth ,lcsh:R ,MED/04 - PATOLOGIA GENERALE ,Biology and Life Sciences ,Cancers and Neoplasms ,Cell Biology ,Cell cycle ,Cell biology ,Isoenzymes ,Oncology ,Cell Processes ,Drug Resistance, Neoplasm ,Colonic Neoplasms ,Immunology ,lcsh:Q ,Tumor Suppressor Protein p53 ,Research Article ,Signal Transduction - Abstract
Glycogen Synthase Kinase-3 alpha (GSK3A) and beta (GSK3B) isoforms are encoded by distinct genes, are 98% identical within their kinase domain and perform similar functions in several settings; however, they are not completely redundant and, depending on the cell type and differentiative status, they also play unique roles. We recently identified a role for GSK3B in drug resistance by demonstrating that its inhibition enables necroptosis in response to chemotherapy in p53-null drug-resistant colon carcinoma cells. We report here that, similarly to GSK3B, also GSK3A silencing/inhibition does not affect cell proliferation or cell cycle but only abolishes growth after treatment with DNA-damaging chemotherapy. In particular, blocking GSK3A impairs DNA repair upon exposure to DNA-damaging drugs. As a consequence, p53-null cells overcome their inability to undergo apoptosis and mount a necroptotic response, characterized by absence of caspase activation and RIP1-independent, PARP-dependent AIF nuclear re-localization. We therefore conclude that GSK3A is redundant with GSK3B in regulating drug-resistance and chemotherapy-induced necroptosis and suggest that inhibition of only one isoform, or rather partial inhibition of overall cellular GSK3 activity, is enough to re-sensitize drug-resistant cells to chemotherapy.
- Published
- 2014
8. Is it possible to reduce recurrences after Altemeier's procedure for complete rectal prolapse? Twenty-year experience in 130 consecutive patients.
- Author
-
Boccasanta P, Venturi M, Agradi S, Calabrò G, Bordoni L, Missaglia C, Favetta U, and Vergani C
- Subjects
- Humans, Quality of Life, Recurrence, Retrospective Studies, Treatment Outcome, Rectal Prolapse surgery
- Abstract
Purpose: In the attempt to understand the reasons for and to find a solution to the high recurrence rate after perineal surgery for complete rectal prolapse, we retrospectively analysed the long-term results of Altemeier's procedure alone, or associated with Trans-Obturator Colonic Suspension (TOCS) in a large series of patients with a median interval of 84 months (range 6-258)., Methods: Medical records of 110 patients undergoing Altemeier with levatorplasty (group 1) and 20 patients submitted to the same procedure associated with TOCS (group 2) for newly diagnosed complete rectal prolapse were reviewed. All patients had been recruited after preoperative clinical examination, SF-36 quality of life, continence score and colonoscopy., Results: Mortality was nil. The overall complication and the recurrence rates were 12.3%, and 15.0% (P= 0.769) and 24.6% and 5.0% (P=0.067) in group 1 and 2, respectively. Twelve patients of group 1 with a recurrence were submitted to a redo-Altemeier, 8 to a redo-Altemeier associated with TOCS, and 6 associated with an anterior coloplasty with a mesh. The only patient of group 2 with a recurrence was submitted to a Hartmann's operation. Preoperative vs postoperative mean (SD) continence score was 15.8 (3.1) and 15.6 (3.3) versus 4.1 (1.8) and 3.9 (1.9) in group 1 and 2, respectively (P < 0.001). All parameters of SF-36 improved after surgery (P<0.01) and no differences between the 2 groups were found CONCLUSIONS: Long-term results confirmed the safety and effectiveness of Altemeier's procedure for the treatment of complete rectal prolapse, with the limit of a non-negligible incidence of anastomotic complications and recurrences. The combination of Altemeier with TOCS showed a positive trend to a reduction of the recurrence rate, not worsening morbidity and outcomes., (© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH, DE part of Springer Nature.)
- Published
- 2021
- Full Text
- View/download PDF
9. A Minimally Invasive Technique for the 1-Stage Treatment of Complex Pelvic Floor Diseases: Laparoscopic-Pelvic Organ Prolapse Suspension.
- Author
-
Boccasanta P, Venturi M, Agradi S, Vergani C, Calabrò G, Missaglia C, Bordoni L, and Longo A
- Subjects
- Adult, Aged, Constipation etiology, Constipation surgery, Female, Genital Diseases, Female etiology, Genital Diseases, Female surgery, Gynecologic Surgical Procedures adverse effects, Gynecologic Surgical Procedures methods, Humans, Middle Aged, Pelvic Organ Prolapse complications, Prospective Studies, Treatment Outcome, Urologic Diseases etiology, Urologic Diseases surgery, Laparoscopy adverse effects, Pelvic Floor Disorders surgery, Pelvic Organ Prolapse surgery
- Abstract
Objective: The aim of this prospective study was to assess the safety and effectiveness of a new single laparoscopic operation devised to relieve obstructed defecation, gynecologic and urinary symptoms in a large series of female patients with multiorgan pelvic prolapse., Methods: We submitted 384 female patients to laparoscopic pelvic organ prolapse suspension operation, a new technique based on suspension of the middle pelvic compartment, by using a polypropylene mesh and followed up 368 of them, with defecography performed 12 months after surgery and a standardized protocol., Results: The 368 patients were followed-up for 36.3 (±4.4) months, Recurrence rate was 4.9% for obstructed defecation syndrome and 3.3% for stress urinary incontinence. Complication rate was 2.9%. The mean period of daily activity resumption was 16.3 days (±4.8 days). Anorectal and urogynecologic symptoms and scores significantly improved after the operation (P < 0.001), with no worsening of anal continence. Incidence of postoperative fecal urgency was 0%. Postoperative defecography showed a significant (P < 0.001) improvement of all parameters in 315 patients (82%). Short Form 36 Health Survey score significantly improved after the operation (P < 0.01). An excellent/good overall Satisfaction Index was reported by 78.0% of patients., Conclusions: In our experience the Laparoscopic-Pelvic Organ Prolapse Suspension seems to be safe and effective as a 1-stage treatment of associated pelvic floor diseases. Randomized studies with an appropriate control group and longer follow-up are now needed to assess the effectiveness of this promising technique., Competing Interests: The authors have declared they have no conflicts of interest., (Copyright © 2019 American Urogynecologic Society. All rights reserved.)
- Published
- 2021
- Full Text
- View/download PDF
10. BTK inhibitors synergise with 5-FU to treat drug-resistant TP53-null colon cancers.
- Author
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Lavitrano M, Ianzano L, Bonomo S, Cialdella A, Cerrito MG, Pisano F, Missaglia C, Giovannoni R, Romano G, McLean CM, Voest EE, D'Amato F, Noli B, Ferri GL, Agostini M, Pucciarelli S, Helin K, Leone BE, Canzonieri V, and Grassilli E
- Subjects
- Agammaglobulinaemia Tyrosine Kinase antagonists & inhibitors, Agammaglobulinaemia Tyrosine Kinase metabolism, Animals, Antineoplastic Combined Chemotherapy Protocols pharmacology, Apoptosis drug effects, Colonic Neoplasms genetics, Colonic Neoplasms pathology, Disease Progression, Drug Resistance, Neoplasm drug effects, Drug Synergism, E2F Transcription Factors metabolism, Fluorouracil administration & dosage, Fluorouracil pharmacology, Genes, p53, Humans, Mice, Nude, Molecular Targeted Therapy methods, Neoplasm Staging, Organoids drug effects, Protein Isoforms antagonists & inhibitors, Protein Isoforms metabolism, Protein Kinase Inhibitors pharmacology, Transforming Growth Factor beta1 metabolism, Tumor Cells, Cultured, Xenograft Model Antitumor Assays methods, Antineoplastic Combined Chemotherapy Protocols therapeutic use, Colonic Neoplasms drug therapy, Protein Kinase Inhibitors administration & dosage
- Abstract
Colorectal cancer (CRC) is the fourth cause of death from cancer worldwide mainly due to the high incidence of drug-resistance. During a screen for new actionable targets in drug-resistant tumours we recently identified p65BTK - a novel oncogenic isoform of Bruton's tyrosine kinase. Studying three different cohorts of patients here we show that p65BTK expression correlates with histotype and cancer progression. Using drug-resistant TP53-null colon cancer cells as a model we demonstrated that p65BTK silencing or chemical inhibition overcame the 5-fluorouracil resistance of CRC cell lines and patient-derived organoids and significantly reduced the growth of xenografted tumours. Mechanistically, we show that blocking p65BTK in drug-resistant cells abolished a 5-FU-elicited TGFB1 protective response and triggered E2F-dependent apoptosis. Taken together, our data demonstrated that targeting p65BTK restores the apoptotic response to chemotherapy of drug-resistant CRCs and gives a proof-of-concept for suggesting the use of BTK inhibitors in combination with 5-FU as a novel therapeutic approach in CRC patients. © 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd., (© 2019 Pathological Society of Great Britain and Ireland. Published by John Wiley & Sons, Ltd.)
- Published
- 2020
- Full Text
- View/download PDF
11. The evolution of transanal surgery for obstructed defecation syndrome: Mid-term results from a randomized study comparing double TST 36 HV and Contour TRANSTAR staplers.
- Author
-
Boccasanta P, Agradi S, Vergani C, Calabrò G, Bordoni L, Missaglia C, and Venturi M
- Subjects
- Adult, Aged, Colonoscopy, Constipation diagnosis, Constipation physiopathology, Defecography, Equipment Design, Female, Follow-Up Studies, Humans, Male, Manometry, Middle Aged, Rectum, Retrospective Studies, Syndrome, Time Factors, Treatment Outcome, Constipation surgery, Defecation physiology, Natural Orifice Endoscopic Surgery methods, Surgical Staplers, Surgical Stapling instrumentation
- Abstract
A randomized study was carried out to compare the mid-term outcome of transanal rectal resection with the CCS-30 TRANSTAR and two TST36 staplers in patients with obstructed defecation syndrome. After selection, patients were randomly assigned to 2 groups:104 underwent a TRANSTAR operation and 104 a transanal rectal resection with two TST36 staplers. Patients were followed up with clinical examination, and defecography. Cumulative complication rate was significantly higher in TRANSTAR operation (P = 0.019). All symptoms and defecographic parameters significantly improved (P < 0.001), without differences. Costs were significantly lower with double TST (P = 0.035). Recurrence rates were 6.2% in TRANSTAR group and 11.4% with double TST (P = 0.206). Two circular TST 36 staplers consent to obtain the same clinical and functional results than the CCS-30, with significantly lower complication rate and costs., (Copyright © 2018 Elsevier Inc. All rights reserved.)
- Published
- 2018
- Full Text
- View/download PDF
12. GSK3A is redundant with GSK3B in modulating drug resistance and chemotherapy-induced necroptosis.
- Author
-
Grassilli E, Ianzano L, Bonomo S, Missaglia C, Cerrito MG, Giovannoni R, Masiero L, and Lavitrano M
- Subjects
- Cell Line, Tumor, Colonic Neoplasms drug therapy, Colonic Neoplasms genetics, Colonic Neoplasms pathology, Glycogen Synthase Kinase 3 genetics, Glycogen Synthase Kinase 3 beta, Humans, Isoenzymes genetics, Isoenzymes metabolism, Necrosis, Tumor Suppressor Protein p53 genetics, Apoptosis, Colonic Neoplasms enzymology, Drug Resistance, Neoplasm, Glycogen Synthase Kinase 3 metabolism
- Abstract
Glycogen Synthase Kinase-3 alpha (GSK3A) and beta (GSK3B) isoforms are encoded by distinct genes, are 98% identical within their kinase domain and perform similar functions in several settings; however, they are not completely redundant and, depending on the cell type and differentiative status, they also play unique roles. We recently identified a role for GSK3B in drug resistance by demonstrating that its inhibition enables necroptosis in response to chemotherapy in p53-null drug-resistant colon carcinoma cells. We report here that, similarly to GSK3B, also GSK3A silencing/inhibition does not affect cell proliferation or cell cycle but only abolishes growth after treatment with DNA-damaging chemotherapy. In particular, blocking GSK3A impairs DNA repair upon exposure to DNA-damaging drugs. As a consequence, p53-null cells overcome their inability to undergo apoptosis and mount a necroptotic response, characterized by absence of caspase activation and RIP1-independent, PARP-dependent AIF nuclear re-localization. We therefore conclude that GSK3A is redundant with GSK3B in regulating drug-resistance and chemotherapy-induced necroptosis and suggest that inhibition of only one isoform, or rather partial inhibition of overall cellular GSK3 activity, is enough to re-sensitize drug-resistant cells to chemotherapy.
- Published
- 2014
- Full Text
- View/download PDF
13. STARR with PPH-01 and CCS30 contour Transtar for obstructed defecation syndrome.
- Author
-
Savastano S, Valenti G, Cavallin F, and Missaglia C
- Subjects
- Adult, Aged, Constipation etiology, Defecography, Female, Humans, Male, Manometry, Middle Aged, Postoperative Period, Prospective Studies, Surveys and Questionnaires, Constipation surgery, Digestive System Surgical Procedures methods, Intestinal Obstruction surgery, Postoperative Complications epidemiology, Rectal Prolapse surgery, Rectocele surgery
- Abstract
Introduction: The stapled transanal rectal resection (STARR) procedure is safe and effective., Objective of the Study: To compare STARR performed with PPH-01 (STARR) and CCS 30 (Transtar)., Materials and Methods: Sixty-four patients underwent STARR for obstructed defecation syndrome (32 STARR and 32 Transtar) and were observed from January 2007 to June 2009. Patients were studied by visit with questionnaires, colonoscopy or barium enema, defecography, and anorectal manometry. Postoperatively they were assessed through visit and questionnaires., Results: All patients improved symptoms without statistical differences. The obstructed defecation syndrome score changed from 13 to 1.8 at 6 months and to 1 at 1 year in the STARR group (P < .05), and the score changed from 15 to 2 at 6 months and to 1 at 1 year in the Transtar group (P < .05). There were no intraoperative complications in the STARR group, but there were 2 dehiscences of suture in the Transtar group. There were no differences with regard to complications., Conclusion: Transtar is a more complex technique with more severe complications. A major resection is not always more effective.
- Published
- 2012
- Full Text
- View/download PDF
14. [Composite tumour of the stomach: a case report and review of the literature].
- Author
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Pase F, Galassi A, Tormen D, Missaglia C, Petrelli G, and D'Amore ES
- Subjects
- Carcinoid Tumor diagnosis, Carcinoid Tumor surgery, Female, Humans, Immunohistochemistry, Middle Aged, Prognosis, Stomach Neoplasms diagnosis, Stomach Neoplasms surgery, Carcinoid Tumor pathology, Stomach Neoplasms pathology
- Abstract
The authors report a rare case of composite tumour of the stomach in a 53-year-old woman documenting the neuroendocrine and glandular features of the tumour by means of immunohistochemical investigations. The authors examine the diagnostic and therapeutic possibilities currently available for the management of this group of rare tumours of uncertain histogenesis. They conclude in favour of elective surgical treatment depending on the site and stage of the tumour in keeping with the data reported in the literature. They also discuss the prognosis of such tumours.
- Published
- 2005
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