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The aim of the present study is to evaluate the level of oxidative stress by quantifying salivary Nitric Oxide in patients with three types of fixed prosthetic: porcelain fused to metal crown; all ceramic; zirconium ceramic. Material and Method. The study was performed on 50 subjects, divided in three groups for each type of fixed dental restauration. Nitric oxide was evaluated by the immunochromatographic method (Nitric Oxide Saliva Test Strips, Berkeley, USA) before and after the permanent cementation of the three different fixed prosthetic. Results.The highest values of Nitric Oxide were quantified in the patients group with porcelain fused to metal crown, after seven days and after six months with statistically significant differences between this values (p=0.0006); there are less statistical significance regarding the values of the same determinations in patients with all ceramics (p=0.02) and no statistically differences regarding the values of these determinations in patients with zirconium ceramic (p=0.32 ). Also, there are statistically significant differences regarding Nitric Oxide values in patients with porcelain fused to metal crown and those with all-ceramic (p=0.0001) and zirconium-ceramic (p=0.0007) after six months demonstrating that the highest level of oxidative stress is produced by metal-ceramic prosthetic materials. Conclusions. The evaluation of the Nitric Oxide level can be used to assess the intensity of oxidative stress in the oral cavity of patients rehabilitated orally with prosthetic works made of different materials.

Background. The purpose of modern dental medicine is to restore the patient’s dental system in order to strengthen health, from a functional, comforting and aesthetic point of view. Methods. The study group consisted of 30 patients to whom we inserted dental implants, which were then rehabilitated by overloading, as follows: 18 patients with fixed metal-ceramic prosthetic (12 women, 6 men) and 12 patients with zirconium-ceramic (5 women, 7 men). Interleukin 1 beta (IL1-) was quantified in all patients after implant insertion at 7, 30 and 90 days, and after overloading at 0, 30 and 90 days. Results. The IL1- values correlate with the depth of the peri-implant pocket at all three moments of implant insertion (r>0.600), with a high statistical significance between the 30- and 90-day determinations (p

Background: The possible histological changes in dental pulp in teeth of diabetic patients are important to be understood, as the clinician will consider the best treatment choice for those teeth, especially if they are affected by decay. The aim of this paper is to assess if there are effects of diabetes-associated hyperglycemia on the nervous and vascular system of the dental pulp. Methods: Twenty-three dental pulp specimens of patients aged 36–70 years old were analyzed. All patients had been diagnosed with type 2 diabetes for at least 5 years. Results: Most of the patients had poorly controlled hyperglycemia, deep caries, but no clinical signs of pulpitis. The histological aspects of pulp specimens included frequently seen inflammatory infiltrate, degeneration of the nerves, thickened blood vessel walls, pulp sclerosis and frequent pulp calcifications, and even small necrotic areas. Conclusion: The analyzed dental pulp specimens of carious teeth of type 2 diabetic patients show fibrotic transformation of the dental pulp, with the presence of calcifications, arteriosclerosis and inflammatory infiltrate. In this situation, the attitude of the dentist in pulp vitality preservation in the case of carious teeth of diabetic patients should be limited.

In the case of old mobile denture wearers, one of the main problems is related to the dentures’ retention and insufficient stability. Our goal was to improve support and stability using a different type of final impression, with different types of impression materials. In this study we chose a number of three complete edentulous patients who presented for complete oral rehabilitation. They were wearing full acrylic dentures with poor support and stability. Complete examination revealed the presence of the flabby ridge. The impression methods for the prosthetic fields with a flabby ridge differ from the classical method by using techniques that involve the use of at least two impression materials with fluid consistency to record all the details of the prosthetic fields in the final impression, this being done in two steps; the impression of the flabby ridge areas must be done without pressure, in its resting position. The impression methods we applied led to the expected results, and the dentures succeeded in offering the patient the desired functional comfort. Using fluid impression material for the flabby ridge in a resting position, and a fenestrated custom tray, offered a good quality in adaptation, maintenance and stability of the final dentures.

Aim of the study Our study objective was to identify a possible association between the hBD-3 level from peri implant crevicular fluid and clinical status in post dental implant patients. Material and methods In this study we comprised 16 patients with dental implants that were examined after 7 and 21 days since dental implant installation and were included in our study based on probing depth (PD) and bleeding on probing (BOP) measurements as follows: 12 subjects with healthy implants and 4 patients with peri-implantitis. The periimplantar crevicular fluid samples were collected by using sterile paper points and the levels of hBD-3 were assayed by using an ELISA kit. Results Our study results show that there are highly significant statistically differences regarding the value of peri-implantar pocket depth between healthy and peri-implantitis patients evaluated after 7 and 21 days after insertion of dental implants (p

Aim of the study In this study we aimed to identify if there is a correlation between the oxidative stress marker 8-hidroxydeoxyguanosine (8-OHdG) from saliva and the orodental clinical status, evaluated by periodontal pocket depth, in aggressive periodontitis. Material and methods The study group was formed by 54 subjects (healthy control, patients with aggressive periodontitis (AgP) and periodontal pocket depth (PPD) between 4 to 5 mm and patients with AgP and PPD between 6-9 mm), which were clinical oro-dental evaluated. We measured the salivary 8-OHdG biomarker by using ELISA method, competitive technique. Results We identified statistically significant differences between the amount of salivary 8-OHdG quantified in healthy group versus AgP patients with PPD 4-5 (p=0.039) and also versus patients with PPD 6-9 (p=0.0001). In our study we also identified a high statistical correlation between the level of salivary 8-OHdG and the PPD of AgP patients (r=0.0446, p=0.005). Conclusions The results of our study demonstrate that salivary 8-OHdG oxidative stress biomarker can be used in the diagnosis and monitoring of aggressive periodontal disease.

Aim of the study This study aims to quantify interleukin-1 (IL-1) and 8-hidroxydeoxyguanosine (8-OHdG) from saliva and to identify a possible correlation between the values of this biomarkers and the periodontal status. Material and methods We made clinical oro-dental evaluation for 40 subjects which were divided in 4 groups: healthy control, gingivitis (G), chronic periodontitis (CP) and aggressive periodontitis (AP). We collected saliva according to the recommendations of the World Health Organization and measured the quantity of the two biomarkers by using ELISA method. Results The values of both biomarkers increased progressively from healthy oro-dental status to the status of CP and AP. There are highly statistically significant differences between the amount of salivary IL-1 quantified in healthy group versus AP patients and between CP and AP (p

Aim of the study The aim of this study is to quantify secretory immunoglobulin A (IgAs) and interleukin 1 (IL1) in saliva, and to establish a possible link between the values of this two parameters and periodontal health. Material and methods Were assessed clinically oro-dental 30 subjects which were divided into 3 groups: healthy, gingivitis and with chronic periodontal disease (CPD). Saliva was collected according to the recommendations of the World Health Organization and quantitative determinations of IgAs and IL-1 were made by ELISA. Results The results show that the values of the two parameters progressively increase in patients with gingivitis and periodontitis, compared to those oro-dental clinically healthy. The study results demonstrate statistically significant differences on values of IL-1 and IgAs in patients with CPD (IgAs 317.92±45.57 mg/mL, IL-1 46.55±38.62 pg/mL) compared with those with gingivitis (IgAs 156.88±31.22 mg/mL, IL-1 40.19±17.04 pg/mL) and healthy (IgAs 151.50±38.73 mg/mL, IL-1 9.84±6.79 IL1 pg/mL). Conclusions IgAs and IL-1 can be used as markers to assess periodontal status.