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3 results on '"Mirabella, Roberto"'

1. Connecting NeRFs, Images, and Text

Author

Ballerini, Francesco, Ramirez, Pierluigi Zama, Mirabella, Roberto, Salti, Samuele, and Di Stefano, Luigi

Subjects
Computer Science - Computer Vision and Pattern Recognition
Abstract

Neural Radiance Fields (NeRFs) have emerged as a standard framework for representing 3D scenes and objects, introducing a novel data type for information exchange and storage. Concurrently, significant progress has been made in multimodal representation learning for text and image data. This paper explores a novel research direction that aims to connect the NeRF modality with other modalities, similar to established methodologies for images and text. To this end, we propose a simple framework that exploits pre-trained models for NeRF representations alongside multimodal models for text and image processing. Our framework learns a bidirectional mapping between NeRF embeddings and those obtained from corresponding images and text. This mapping unlocks several novel and useful applications, including NeRF zero-shot classification and NeRF retrieval from images or text., Comment: Accepted at CVPRW-INRV 2024

Published
2024

3. Membrane fatty acid heterogeneity of leukocyte classes is altered during in vitro cultivation but can be restored with ad-hoc lipid supplementation

Author

Elisa Assirelli, Erminia Mariani, Roberto Mirabella, Alexandros Chatgilialoglu, Simona Neri, Paola Poggi, Paolo Dolzani, Philip C. Calder, Poggi, Paola, Mirabella, Roberto, Neri, Simona, Assirelli, Elisa, Dolzani, Paolo, Mariani, Erminia, Calder, Philip C., and Chatgilialoglu, Alexandros

Subjects
CD4-Positive T-Lymphocytes, Chromatography, Gas, Membrane Fluidity, Membrane lipids, Endocrinology, Diabetes and Metabolism, Primary Cell Culture, Cell, Clinical Biochemistry, CD8-Positive T-Lymphocytes, Biology, Monocyte, Jurkat cells, Monocytes, Cell membrane, Jurkat Cells, Membrane Lipids, Endocrinology, In vivo, Leukocytes, medicine, Membrane fluidity, Humans, Lymphocytes, Lipid supplementation, Biochemistry, medical, chemistry.chemical_classification, B-Lymphocytes, Research, Cell Membrane, Fatty Acids, Biochemistry (medical), Fatty acid, Leukocyte, Cell cultures, Culture Media, medicine.anatomical_structure, Biochemistry, chemistry, Cell culture, Lymphocyte, Membrane lipid composition, Membrane fatty acid profile
Abstract

Background: The cell membrane is a primary and fundamental player in most cellular processes, and fatty acids form a major structural component of cell membranes. The aim of this study was to compare the membrane fatty acid profiles of different human blood leukocytes and selected cell lines, to identify the effects of in vitro culture on fatty acid profiles, and to test medium supplements for their effect on fatty acid profiles.Methods: Different classes of leukocytes were isolated from human blood and their membrane fatty acid profiles were analysed and compared. After culturing in vitro immortalised and primary leukocytes, membrane fatty acids were analysed and compared. Finally, different lipid formulations were developed and used for supplementing leukocytes in vitro in an effort to maintain the in vivo fatty acid profile. Descriptive and analytical tests were performed to compare the obtained fatty acid profiles.Results: Membrane fatty acid profiles of primary human CD4+ T-lymphocytes, CD8+ T-lymphocytes, B-lymphocytes and monocytes differed. Moreover, there were differences among Jurkat, Raji and THP-1 cell lines and the corresponding primary leukocyte classes, as well as between freshly prepared and in vitro cultured primary lymphocytes. A lipid supplement was able to maintain cultured Jurkat cells with a membrane fatty acid profile almost identical to that of the primary CD4+ T-lymphocytes. Finally, variations in the lipid supplement composition enabled the development of Jurkat cells with different membrane fatty acid profiles characterising different physiological or pathological human conditions.Conclusions: Each leukocyte class has its own specific membrane fatty acid profile in vivo. Cultured primary leukocytes and immortalized leukocytic cells display different membrane fatty acid profiles when compared to their respective in vivo counterparts. The membrane fatty acid composition of cultured cells can be restored to reflect that of the corresponding in vivo condition through use of optimised lipid supplementation. Typical physiological or pathological leukocyte membrane fatty acid profiles can be obtained by tuning in vitro fatty acid supplementation.

Published
2015

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