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Your search keyword '"Minoo Vahedi Raad"' showing total 3 results
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3 results on '"Minoo Vahedi Raad"'

1. The impact of mitochondrial impairments on sperm function and male fertility: a systematic review

Author

Minoo Vahedi Raad, Amir Masoud Firouzabadi, Maryam Tofighi Niaki, Ralf Henkel, and Farzaneh Fesahat

Subjects
Assisted reproductive technology, In vitro fertilization, Male infertility, Mitochondria, Mitochondrial DNA, Mitochondrial membrane potential, Gynecology and obstetrics, RG1-991, Reproduction, QH471-489
Abstract

Abstract Background Besides adenine triphosphate (ATP) production for sustaining motility, the mitochondria of sperm also host other critical cellular functions during germ cell development and fertilization including calcium homeostasis, generation of reactive oxygen species (ROS), apoptosis, and in some cases steroid hormone biosynthesis. Normal mitochondrial membrane potential with optimal mitochondrial performance is essential for sperm motility, capacitation, acrosome reaction, and DNA integrity. Results Defects in the sperm mitochondrial function can severely harm the fertility potential of males. The role of sperm mitochondria in fertilization and its final fate after fertilization is still controversial. Here, we review the current knowledge on human sperm mitochondria characteristics and their physiological and pathological conditions, paying special attention to improvements in assistant reproductive technology and available treatments to ameliorate male infertility. Conclusion Although mitochondrial variants associated with male infertility have potential clinical use, research is limited. Further understanding is needed to determine how these characteristics lead to adverse pregnancy outcomes and affect male fertility potential.

Published
2024
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2. Effects of adverse semen parameters on total oxidation status and DNA/chromatin integrity

Author

Maryam Imani, Minoo Vahedi Raad, Maliheh Afsari, and Farzaneh Fesahat

Subjects
Chromatin quality, DNA defects, Male infertility, Oxidative stress, Sperm, Diseases of the genitourinary system. Urology, RC870-923
Abstract

Abstract Background Infertility is a major health problem that affects 7% of the men’s population. Oxidative stress (OS) plays a significant role in the pathophysiology of male infertility. The purpose of this study comparatively evaluated the total anti-oxidation status and DNA/chromatin integrity in semen samples among different infertile men’s groups compared with the normozoospermic men. Methods This cross-sectional study contains four experimental groups, including teratozoospermia (Exp I), asthenoteratozoospermia (Exp II), oligoasthenoteratozoospermia (Exp III), and azoospermia (Exp IV), as well as the control group of normozoospermic men. The total antioxidant capacity (TAC) and total oxidant status (TOS) were assessed by applying the enzyme-linked immunosorbent assay. The chromatin/DNA damage was assessed in semen samples of all study groups by applying chromomycin A3 (CMA3) and toluidine blue (TB) staining methods. Results The results showed significantly higher proportions of TB+ and CMA3 positive sperm in all experimental groups compared to controls (P

Published
2023
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3. Altered methyltransferase gene expression, mitochondrial copy number and 4977‐bp common deletion in subfertile men with variable sperm parameters

Author

Minoo Vahedi Raad, Farzaneh Fesahat, Ali Reza Talebi, Mohammad Hosseini‐Sharifabad, Ali Zare Horoki, Maliheh Afsari, and Abolghasem Abbasi Sarcheshmeh

Subjects
Male, DNA, Complementary, DNA Copy Number Variations, Urology, Gene Expression, Methyltransferases, General Medicine, DNA, Mitochondrial, Spermatozoa, Endocrinology, Semen, Sperm Motility, Humans, RNA, RNA, Messenger, Infertility, Male
Abstract

Semen parameters have been found to predict reproductive success poorly and are the most prevalent diagnostic tool for male infertility. There are few conflicting reports regarding the correlation of DNMT genes expression, mitochondrial DNA copy number (mtDNAcn) and deletion (mtDNAdel) with different sperm parameters. To investigate DNMT mRNA level, mtDNAcn and deletion in infertile men, with different sperm parameters, compared with fertile men, semen samples from 30 men with unknown male infertility and normal sperm parameters (experimental group I), 30 infertile patients with at least two abnormal sperm parameters (experimental group II) and 30 fertile normozoospermic men (control group) were collected. After semen analysis, total RNA and DNA were extracted. The isolated DNA was used for assessing the respective mtDNAcn and the presence of common 4977 bp deletion in mtDNA by applying real-time quantitative PCR and multiplex PCR, respectively. Synthesized cDNA from total RNAs was used to quantify DNMT1, DNMT3A and DNMT3B transcripts in study groups by using real-time quantitative reverse-transcription PCR. Significantly higher proportions of mtDNAcn were found in experimental group II. DNMT1 was significantly downregulated in both experimental groups and 4977 bp deletion was not detected. Progressive motility and normal morphology were significantly and negatively correlated with mtDNAcn. A significant positive correlation was detected between sperm parameters and DNMT1 mRNA levels. In conclusion, infertile men with different sperm parameter qualities showed elevated mtDNA content. Abnormal sperm parameters associated with DNMT1 gene expression indicate the possibility of changes in some epigenetic aspects of spermatogenesis in subfertile men with different sperm parameters.

Published
2022

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