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Medium-chain triglyceride (MCT) and eicosapentaenoic acid (EPA) has been widely applied in nutritional supplementation. However, when administered individually or mixed, they were unable to maximize their nutritional value. Hence, EPA-rich medium- and long-chain triacylglycerol (MLCT) was synthesized from MCT and EPA-rich fish oil (FO) by enzymatic transesterification. The fatty acids in triglyceride (TAG) were rearranged and resulted in significant changes in TAG profiles compared to physical mixture of MCT and FO (PM). EPA-containing MML (MML, MLM and LMM) and LLM (LLM, LML and MLL) type TAGs account for 70.21%. The fate of different oils (MCT, FO, PM, and MLCT) across the gastro-intestinal tract was subsequently simulated by an in vitro digestion model. The results showed that the physical and structural characteristics of different oils during digestion depended upon the oil type and the microenvironment they were in. The degree of hydrolysis for MLCT was higher than that for the other three oils. FFAs release level was presented in the following order: MLCT＞MCT＞PM＞FO. This can be attributed to the composition and positional distribution of fatty acids in TAGs. What is more, LCFAs (EPA) in MLCT mainly existed in the form of sn-2 MAG, which was conducive to their subsequent absorption and transport.These results may aid in the future rational design of structural lipids, thereby regulating lipid digestion and maximizing the nutritional value of oils.

Coronavirus disease (COVID-19) is an infectious disease caused by severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2), with strong contagiousness, high susceptibility and long incubation period. cell entry by SARS-CoV-2 requires the binding between the receptor-binding domain of the viral spike protein and the cellular angiotensin-converting enzyme 2 (ACE2). Here, we briefly reviewed the mechanisms underlying the interaction between SARS-CoV-2 and ACE2, and summarized the latest research progress on SARS-CoV-2 neutralizing monoclonal antibodies and nanobodies, so as to better understand the development process and drug research direction of COVID-19. This review may facilitate understanding the development of neutralizing antibody drugs for emerging infectious diseases, especially for COVID-19.

The subunit of tropomyosin (α-TM) from Haliotis discus hannai is an important allergen. The methods to reduce the immunoreactivity of α-TM are worth investigating. Thus, this study confirmed the reacted conditions of α-TM with transglutaminase (TG)-catalyzed cross-linking reaction, TG-catalyzed glycosylation, and glycation. Three processing technologies reduced significantly the contents of α-helix and hydrophobic force of α-TM and increased the surface hydrophobicity. A serological experiment confirmed that the glycated α-TM with xylose showed the lowest IgG/IgE-binding capacity. The inhabitation dot blot displayed that five epitope peptides could bind with the site-specific IgE prepared by the glycated α-TM. Three in nine glycated sites (M68, N202, and N203) were verified to modify-two epitopes (L-HTM-3 and L-HTM-7) of α-TM, which affected the immunoreactivity of α-TM during glycation. These results indicated that glycation would be desired for developing hypo-allergenic abalone products.

In recent decades, the treatment of autoimmune diseases has moved from the use of hormones and conventional immunosuppressive drugs to biological agents. B cell proliferation and maturation play crucial roles in the development of autoimmune diseases. The tumor necrosis factor superfamily ligand B cell activating factor (BAFF) and its receptor mediate B cell survival through regulating signaling pathways. Therefore, BAFF and its receptors are important therapeutic targets for the treatment of autoimmune diseases. This review describes the mechanism of BAFF and its receptor in the human body system and introduces the latest views on how over-activation of BAFF pathway promotes the development of autoimmune diseases including systemic lupus erythematosus, Sjogren's syndrome, and rheumatoid arthritis. In connection to the treatment of the above three diseases, this review discusses the clinical trials and application status of three BAFF-targeting antibody drugs, including Belimumab, Tabalumab and Atacicept. Finally, this review proposes new strategies that targeting the BAFF pathway to provide a new treatment for autoimmune diseases.

The resistance in microorganisms against many conventional antibiotics has become a serious global health problem. However, antibacterial drug delivery materials are still limited in toxicity, short efficacy and reducing inflammation. The novel and natural Pickering emulsions stabilized by antimicrobial peptide nanoparticles were tested as promising platforms to control bacterial resistance development. The parasin I interacted or conjugated with lecithin or chitosan and formed nanoparticles encapsulated by Pickering emulsion. The protonation and deprotonation of amino groups in chitosan and parasin I resulted in nanoparticles in different aggregate states and changed emulsion stability. Moreover, the Pickering emulsion could induce severe bacterial agglomeration on both Gram-positive and Gram-negative bacteria than parasin I through the membrane disintegration mechanism. Furthermore, the Pickering emulsion could alleviate the cytotoxicity of human liver cells and hemolytic activity in rat blood cells. In combination with the lack of acute cytotoxicity in Kunming mice and milder, more effective anti-inflammatory effect in peritonitis demonstrated for these Pickering emulsions, especially chitosan peptide-embedded nanoparticles Pickering emulsion, a potential role in combating multidrug resistant bacteria in biomedical applications.

Medium-chain triglyceride (MCT) and eicosapentaenoic acid (EPA) have been widely applied in nutritional supplementation. However, when administered individually or mixed, they were unable to maximize their nutritional value. Hence, EPA-rich medium- and long-chain triacylglycerol (MLCT) was synthesized from MCT and EPA-rich fish oil (FO) by enzymatic transesterification. The fatty acids in triglyceride (TAG) were rearranged which resulted in significant changes in TAG profiles compared to the physical mixture of MCT and FO (PM). EPA-containing MML (MML, MLM and LMM) and LLM (LLM, LML and MLL) type TAGs account for 70.21%. The fate of different oils (MCT, FO, PM, and MLCT) across the gastrointestinal tract was subsequently simulated using an

Seaweeds are important "blue food" and some seaweeds have been demonstrated that have the immunoregulation activities and benefit for human health. However, which immune cell subsets are targeted and how immune processes are regulated by those seaweeds remain poorly understood. Here we identified sulfated oligosaccharide of Gracilaria lemaneiformis (GLSO) inhibits IFNγ production by T cells in ovalbumin (OVA) immunized mice and in vitro activation system of OVA-specific CD4

Based on an analysis of 51-year (1965–2015) data, the influence of El Nino-Southern Oscillation (ENSO) events on tropical cyclone (TC) activity is examined over the western North Pacific (WNP). The total number of TCs formed in the entire WNP reduces by about 3.4 TCs per year in La Nina years, whereas TCs have an equivalent genesis number between El Nino years and climatology. During El Nino years, the frequency of TC formation increases remarkably in the southeast quadrant (140°E–180°, 0°–17°N) and decreases in the northwest quadrant (120°–140°E, 17°–30°N). During La Nina years, TCs tend to form in the northwest and southwest quadrants (120°-140°E, 0°–17°N) quadrants. TCs tend to become long-lived in the peak season (from July to September) of El Nino years and during strong El Nino events. TC genesis shows a southeastward positive shift in terms of lifetime and intensity during El Nino years, thus more super TCs (winds ≥ 58.64ms−1) are formed in the southeast quadrant. Further analysis using the genesis potential index (GPI) indicates that the interannual variations in the TC genesis and track are significantly influenced by a combination of large-scale dynamic and thermodynamic conditions.

The iTRAQ-based quantitative proteomic strategy was used to reveal the effects of magnetic nanoparticles plus microwave thawing (MNPMT) on the largemouth bass fillets quality. A total number of 47 proteins were identified as differentially abundant proteins (DAPs) in fillets treated by microwave thawing (MT), while 13 DAPs were in MNPMT. Bioinformatics analysis of Gene ontology (GO) enrichment, Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment, Protein-protein interaction (PPI) and subcellular localization prediction of DAPs showed that many DAPs were involved in protein structure, metabolic enzymes, protein turnover and so on. The correlation analyses indicated that 14 DAPs were significantly related with the fillet quality traits. Besides, several discovered proteins might be potential protein markers for pH, color, cooking loss and centrifuge loss of thawed largemouth bass fillet quality. These results provided a further understanding of quality stability of largemouth bass fillet treated by MNPMT from the perspective of proteomics.

The influence of ultrasonics combined with microwave thawing (UMT) and ultrasonics combined with far-infrared thawing (UIT) on the water migration and protein denaturation of red drum were studied. Five treatments were used: ultrasonics thawing (UT), microwave thawing (MT), far-infrared thawing (IT), UIT and UMT were used for thawing red drum filets. Moisture migration and protein aggregation were studied using nuclear magnetic resonance (NMR) and particle size, respectively. Raman spectra and fluorescence spectra were used to study the secondary and tertiary structure of protein. SEM was done to observe the fiber microstructure. The results showed that UMT and UIT can maintained protein stability more than other thawing methods and retained the fiber structure of the muscle. Besides, immobilized water in fiber bundles network also had no significant changes compared with fresh samples. Thus, ultrasonics combined with far infrared or microwave thawing were used to decrease protein denaturation and water migration during the thawing of Red drum fillets.

Serious quality deterioration can occur with suboptimal thawing, and thus innovative thawing technologies may have an important role in improving the final quality of frozen foods. In recent years, although several new thawing technologies have been extensively studied, such as ultra-high pressure assisted thawing, ultrasound-assisted thawing, high-voltage electrostatic field thawing, ohmic thawing, and radio frequency thawing, more research is needed to make them more applicable to thawing of food industrially. A better evaluation of the impact of thawing is needed to help move new thawing technologies forward. This review discusses the principles involved, the applications to different types of foods, modeling of the various processes, new evaluation techniques, and patents obtained for the different systems. The benefits and weaknesses of these systems are also discussed to provide a more complete review of these new thawing techniques. This review will, hopefully, encourage additional work that may help reach the goal of having better food thawing systems.

The volatile flavor characteristics of largemouth bass (Micropterus salmoides) fillets thawed by magnetic nanoparticles plus microwave thawing (MNPMT) were compared with those of fresh samples thawed by chill storage thawing (CST) and microwave thawing (MT). The strategies of headspace solid-phase microextraction (HS-SPME), gas chromatography mass spectrometry (GC-MS), gas chromatography-olfactometry (GC-O), aroma extract dilution analysis (AEDA), and sensory evaluation were applied to analyze the aroma-active compounds. The results of GC-MS showed that 24 kinds of volatile compounds existed in largemouth bass fillets. Orthogonal partial least squares discriminate analysis (OPLS-DA) (R2Y = 0.979, R2Y = 0.98, Q2 = 0.695) showed ethanol, hexanal, 2,6,10,14-tetramethyl-pentadecane, and (E)-9-octadecenoic acid were responsible for the flavor differences in MNPMT, MT, and CST. In GC-O measurement, 14 kinds of volatile compounds were detected, and MNPMT had similar aroma-active compounds with the fresh sample. The grassy (score = 1.58) and leafy (score = 0.75) odors were weak in MT, but the fishy odors (score = 1.5) were strong. All results showed the MNPMT samples had less changes in aroma composition compared with the fresh sample.

Recrystallisation often occurs in the freeze‐thaw cycles and results in poor food quality. This study was undertaken to analyse the effects of recrystallisation on the changes of protein conformation and moisture migration within red seabream fillets after the freeze‐thaw cycles. The UV second‐derivative spectroscopy, intrinsic fluorescence measurement and Raman spectroscopy were employed to evaluate the protein conformational changes. The Low‐field NMR spectra analysis was used to evaluate moisture migration. The results indicated that the freeze‐thaw cycles altered the secondary and tertiary structure of proteins. It was noticed that interactions between water and protein molecules were decreased gradually and eventually induced the thermal instability of the myosin molecules. The immobilised water content declined and the free water content increased. There were significant destructions in fillet muscle microstructure. These observations unambiguously reflect how the recrystallisations affect the protein conformation and moisture migration patterns of red seabream fillets during freeze‐thaw cycles.

In this work, naringenin loaded β-cyclodextrin and carbon quantum dots composite nanoparticles were successfully fabricated. The results showed that incorporation of carbon quantum dots not only enhanced antioxidant activities of nanoparticles but also improved encapsulation efficiency of naringenin. Further, the formation of composite nanoparticles was confirmed by a series of characterization methods. The zeta-potential and Fourier transform infrared spectroscopy data proved that electrostatic interaction and hydrogen bonding are dominant forces to form nanoparticles. X-Ray Diffraction experiment revealed that the material state of the formed naringenin-β-CD-CQDs nanoparticles is amorphous in opposition to the crystalline state of naringenin, β-CD and naringenin-β-CD inclusion complex. Finally, antioxidant activity analyses against DPPH, ABTS+ and Fe2+ chelating, showed an enhanced antioxidant activity of the formed composite nanoparticles compared to their constituents. These results indicated that naringenin can be effectively entrapped in β-cyclodextrin and carbon quantum dots, forming composite nanoparticles with improved antioxidant properties.

Super-resolution face image is the basis of high detection rate in face recognition. In order to meet the requirements of super-resolution image in face recognition, aiming at the problem of texture loss of super-resolution image under high-frequency features, a face image reconstruction method based on wavelet transform and super-resolution generative adversarial network (SRGAN) is proposed to reduce the impact of low-resolution image caused by imaging hardware, network bandwidth and sampling environment on face recognition accuracy. Firstly, the wavelet transform algorithm is used to preprocess the low-resolution face image to extract the detailed texture features of the face image under different frequencies. Then, GAN is used to learn the prior knowledge of wavelet coefficients, and the identity preserving constraint is applied to the output image, and the perceptual loss function of the fusion wavelet coefficients is realized. Finally, the deep learning model based on SRGAN is used to obtain high-resolution face images. Experimental results show that the method can achieve super-resolution restoration of low-resolution face images and meet the requirements of face recognition accuracy.

In order to evaluate the effects of magnetic nanoparticles plus microwave (MNPMT) or far-infrared (MNPFT) thawing on protein conformation changes and moisture migration of red seabream (Pagrus major) fillets, cold storage thawing, microwave thawing, MNPMT, far-infrared thawing and MNPFT were subjected to red seabream fillets. DSC and dynamic rheology were conducted to analyze physicochemical changes. Raman spectra before and after isotope H/D exchange, intrinsic fluorescence and UV second derivative spectra were carried out to explore myofibrillar protein structural changes. Low-field NMR was used to test water migration. SEM was performed to observe fiber microstructure. Results indicated that MNPMT and MNPFT both had a desirable thermal stability and gelation property. Besides, both showed stable protein secondary and tertiary structure. Immobilized water in them also had no significant changes compared with fresh sample. This research provided some novel thawing technologies for application in food industry.

Ultrasound assisted vacuum thawing (UVT) or microwave vacuum thawing (MVT) with red seabream fillets were compared to fresh, chill storage thawing, vacuum thawing, microwave thawing and ultrasound thawing. The thermal stability and gelation properties were studied with DSC and dynamic rheology, respectively. Raman spectra before and after H/D isotope exchange and intrinsic fluorescence were used to measure protein secondary and tertiary structure. Low-field NMR was done to measure water migration. The two thawing techniques both retained actin thermal stability and generally retained more stable tertiary structures than the other thawing methods. MVT showed a desirable viscoelasticity of muscle proteins and UVT had a relatively stable secondary structure. There were no significant changes in free water. Thus, UVT and MVT could be used to improve the physicochemical properties of proteins during thawing of fillets.

The present study investigated the effects of CS@Fe

To investigate the production mechanism of semicarbazide from protein in Chinese softshell turtles at different drying temperatures, the tandem mass tags -tagged protein quantification technique and tandem mass spectrometry were used to screen the differentially abundant proteins of Chinese softshell turtles. Results showed that the protein content in the shells showed a decreasing trend with the increase of drying temperature, and the difference between the protein contents at 70 ℃ and 100 ℃ was significant (p

The present study investigated the effects of CS@Fe3O4 nanoparticles combined with microwave or far infrared thawing on microbial diversity of red seabream (Pagrus major) fillets in terms of thawing loss, pH, TVB-N, classical microbiological enumeration and high-throughput sequencing, and the same parameters were also studied for 24 h after thawing. Four thawing methods were used: microwave thawing (MT), far-infrared thawing (FT), CS@Fe3O4 nanoparticles combined with microwave thawing (CMT) and CS@Fe3O4 nanoparticles combined with far-infrared thawing (CFT). The results showed that CFT and CMT had lower values of pH and TVB-N compared to the FT and MT. Based on conventional microbial count analysis, CFT and CMT samples also maintained lower TVC, pseudomonas and LAB counts. Using high-throughput sequencing analysis, Compared with FT and MT, CFT and CMT samples showed a significant decrease in the proportion of the Pseudomonadaceae flora. However, the proportion of Pseudomonas, Bacillaceae and Thermaceae also increased significantly after 24 h of storage, which indicated that become the predominant microbiota in red seabream (Pagrus major) fillets.

The effects of magnetic nanoparticles combined with microwave thawing (MNPMT) and magnetic nanoparticles combined with far-infrared thawing (MNPFT) on the freshness and amines content of red seabream fillets were investigated. Four thawing methods were used: microwave thawing (MT), far-infrared thawing (FIRT), magnetic nanoparticles combined with microwave thawing (MNPMT) and magnetic nanoparticles combined with far-infrared thawing (MNPFT). The quality of the fresh sample, the sample after thawing and thawed sample after being stored for 6 h were compared by measuring the freshness indexes such as the volatile amino nitrogen content, thiobarbituric acid content, pH value, total number of viable colonies, and the food safety evaluation indexes such as the formaldehyde, dimethylamine, trimethylamine and putrescine contents. The results showed that MNPFT and MNPMT can maintain better freshness than microwave and far-infrared thawing, and the increase in amines content was significantly inhibited.

This study investigated the effects of different thawing methods on the quality of largemouth bass. The viscoelastic properties of proteins in Ultrasonics combined with microwave thawing (UMT) and Ultrasonics combined with far-infrared thawing (UIT) samples were closer to FS, which was an indication of good gelation and gel quality. Zeta potential, Raman spectra before and after H/D isotope exchange were used to measure the protein aggregation and the secondary structure of protein. Fourier transform Raman spectra and Raman spectra have been used to investigate the chemical changes in fish lipid during lipid oxidation. Results indicated that both UMT and UIT showed protein stability and gelation property. Thus, it was proved that UMT and UIT could be potentially used to improve the fish quality during thawing process.