1. High prevalence of plasmid-mediated quinolone resistance determinants in commensal members of the Enterobacteriaceae in Ho Chi Minh City, Vietnam.
- Author
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Minh Vien LT, Baker S, Phuong Thao LT, Phuong Tu LT, Thu Thuy C, Thu Nga TT, Minh Hoang NV, Campbell JI, Minh Yen L, Trong Hieu N, Vinh Chau NV, Farrar J, and Schultsz C
- Subjects
- Adult, Anti-Bacterial Agents pharmacology, Bacterial Proteins genetics, Bacterial Proteins metabolism, Carrier State, Child, Child, Preschool, Enterobacteriaceae genetics, Gene Expression Regulation, Bacterial physiology, Humans, Infant, Mutation, Vietnam epidemiology, Drug Resistance, Multiple, Bacterial genetics, Enterobacteriaceae drug effects, Enterobacteriaceae Infections epidemiology, Enterobacteriaceae Infections microbiology, Plasmids genetics, Quinolones pharmacology
- Abstract
Antimicrobial-resistant pathogenic members of the Enterobacteriaceae are a well-defined global problem. We hypothesized that one of the main reservoirs of dissemination of antimicrobial resistance genes in Vietnam is non-pathogenic intestinal flora, and sought to isolate antimicrobial-resistant organisms from hospitalized patients and non-hospitalized healthy individuals in Ho Chi Minh City. The results identified substantial faecal carriage of gentamicin-, ceftazidime- and nalidixic acid-resistant members of the Enterobacteriaceae in both hospitalized patients and non-hospitalized healthy individuals. A high prevalence of quinolone resistance determinants was identified, particularly the qnrS gene, in both community- and hospital-associated strains. Furthermore, the results demonstrated that a combination of quinolone resistance determinants can confer resistance to nalidixic acid and ciprofloxacin, even in the apparent absence of additional chromosomal resistance mutations in wild-type strains and laboratory strains with transferred plasmids. These data suggest that intestinal commensal organisms are a significant reservoir for the dissemination of plasmid-mediated quinolone resistance in Ho Chi Minh City.
- Published
- 2009
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