102 results on '"Minghui Gu"'
Search Results
2. Application of the New Irrigation Protocol to Reduce Recurrence Rate in the Management Of Periprosthetic Joint Infection
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Xiaoyu Wu, Weishen Chen, Rong Rong, Baiqi Pan, Xuantao Hu, Linli Zheng, Aerman Alimu, Chenghan Chu, Yucheng Tu, Ziji Zhang, Yongyu Ye, Minghui Gu, and Puyi Sheng
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Irrigation protocol ,Periprosthetic joint infection ,Recurrence ,Revision ,Staphylococcus aureus ,Orthopedic surgery ,RD701-811 - Abstract
Objective Irrigation is a conventional treatment for acute and chronic periprosthetic joint infections (PJI). However, there has been no unified standard for irrigation during surgery for PJI in the past, and the efficacy is uncertain. The purpose of this study is to create a new irrigation protocol to enhance the infection control rate and reduce the postoperative recurrence rate of PJI patients. Methods We conducted a single‐institution retrospective review with a total of 56 patients who underwent revision total hip or knee arthroplasties due to PJI from January 2011 to January 2022. Conventional irrigation (CI) was used in 32 cases, and standard operating procedure of irrigation (SOPI) was used in 24. The CI protocol carries out an empirical irrigation after debridement, which is quite random. Our SOPI protocol clearly stipulates the soaking concentration and time of hydrogen peroxide and povidone‐iodine. The irrigation is carried out three times, and tissue samples are taken from multiple parts before and after irrigation, which are sent for microbial culture. The important statistical indicators were the rate of positive microbiological culture and postoperative recurrence rate with an average follow‐up of 24 average months. Results The drainage volume was lower in the SOPI group than in the CI group on postoperative day 3 (p
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- 2024
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3. Research progress of chilled meat freshness detection based on nanozyme sensing systems
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Guangchun Song, Cheng Li, Marie-Laure Fauconnier, Dequan Zhang, Minghui Gu, Li Chen, Yaoxin Lin, Songlei Wang, and Xiaochun Zheng
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Chilled meat ,Freshness indicators ,Enzyme-like catalysis ,Nanozyme sensing systems ,Nutrition. Foods and food supply ,TX341-641 ,Food processing and manufacture ,TP368-456 - Abstract
It is important to develop rapid, accurate, and portable technologies for detecting the freshness of chilled meat to meet the current demands of meat industry. This report introduces freshness indicators for monitoring the freshness changes of chilled meat, and systematically analyzes the current status of existing detection technologies which focus on the feasibility of using nanozyme for meat freshness sensing detection. Furthermore, it examines the limitations and foresees the future development trends of utilizing current nanozyme sensing systems in evaluating chilled meat freshness. Harmful chemicals are produced by food spoilage degradation, including biogenic amines, volatile amines, hydrogen sulfide, and xanthine, which have become new freshness indicators to evaluate the freshness of chilled meat. The recognition mechanisms are clarified based on the special chemical reaction with nanozyme or directly inducting the enzyme-like catalytic activity of nanozyme.
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- 2024
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4. Magnetism and berry phase manipulation in an emergent structure of perovskite ruthenate by (111) strain engineering
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Zhaoqing Ding, Xuejiao Chen, Zhenzhen Wang, Qinghua Zhang, Fang Yang, Jiachang Bi, Ting Lin, Zhen Wang, Xiaofeng Wu, Minghui Gu, Meng Meng, Yanwei Cao, Lin Gu, Jiandi Zhang, Zhicheng Zhong, Xiaoran Liu, and Jiandong Guo
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Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Atomic physics. Constitution and properties of matter ,QC170-197 - Abstract
Abstract The interplay among symmetry of lattices, electronic correlations, and Berry phase of the Bloch states in solids has led to fascinating quantum phases of matter. A prototypical system is the magnetic Weyl candidate SrRuO3, where designing and creating electronic and topological properties on artificial lattice geometry is highly demanded yet remains elusive. Here, we establish an emergent trigonal structure of SrRuO3 by means of heteroepitaxial strain engineering along the [111] crystallographic axis. Distinctive from bulk, the trigonal SrRuO3 exhibits a peculiar XY-type ferromagnetic ground state, with the coexistence of high-mobility holes likely from linear Weyl bands and low-mobility electrons from normal quadratic bands as carriers. The presence of Weyl nodes are further corroborated by capturing intrinsic anomalous Hall effect, acting as momentum-space sources of Berry curvatures. The experimental observations are consistent with our first-principles calculations, shedding light on the detailed band topology of trigonal SrRuO3 with multiple pairs of Weyl nodes near the Fermi level. Our findings signify the essence of magnetism and Berry phase manipulation via lattice design and pave the way towards unveiling nontrivial correlated topological phenomena.
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- 2023
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5. Macrophages-derived exosomes modulates wear particle-induced osteolysis via miR-3470b targeting TAB3/NF-κB signaling
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Baiqi Pan, Ziji Zhang, Xiaoyu Wu, Guoyan Xian, Xuantao Hu, Minghui Gu, Linli Zheng, Xiang Li, Lingli Long, Weishen Chen, and Puyi Sheng
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Aseptic prothesis loosening ,Exosome ,Non-coding RNA ,Macrophage ,Inflammatory osteolysis ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biology (General) ,QH301-705.5 - Published
- 2023
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6. SARS-CoV-2 envelope protein triggers depression-like behaviors and dysosmia via TLR2-mediated neuroinflammation in mice
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Wenliang Su, Jiahang Ju, Minghui Gu, Xinrui Wang, Shaozhuang Liu, Jiawen Yu, and Dongliang Mu
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COVID-19 ,Depression ,Dysosmia ,E protein ,TLR2 ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Abstract Background Depression and dysosmia have been regarded as primary neurological symptoms in COVID-19 patients, the mechanism of which remains unclear. Current studies have demonstrated that the SARS-CoV-2 envelope (E) protein is a pro-inflammatory factor sensed by Toll-like receptor 2 (TLR2), suggesting the pathological feature of E protein is independent of viral infection. In this study, we aim to ascertain the role of E protein in depression, dysosmia and associated neuroinflammation in the central nervous system (CNS). Methods Depression-like behaviors and olfactory function were observed in both female and male mice receiving intracisternal injection of E protein. Immunohistochemistry was applied in conjunction with RT-PCR to evaluate glial activation, blood–brain barrier status and mediators synthesis in the cortex, hippocampus and olfactory bulb. TLR2 was pharmacologically blocked to determine its role in E protein-related depression-like behaviors and dysosmia in mice. Results Intracisternal injection of E protein evoked depression-like behaviors and dysosmia in both female and male mice. Immunohistochemistry suggested that the E protein upregulated IBA1 and GFAP in the cortex, hippocampus and olfactory bulb, while ZO-1 was downregulated. Moreover, IL-1β, TNF-α, IL-6, CCL2, MMP2 and CSF1 were upregulated in both cortex and hippocampus, whereas IL-1β, IL-6 and CCL2 were upregulated in the olfactory bulb. Furtherly, inhibiting microglia, rather than astrocytes, alleviated depression-like behaviors and dysosmia induced by E protein. Finally, RT-PCR and immunohistochemistry suggested that TLR2 was upregulated in the cortex, hippocampus and olfactory bulb, the blocking of which mitigated depression-like behaviors and dysosmia induced by E protein. Conclusions Our study demonstrates that envelope protein could directly induce depression-like behaviors, dysosmia, and obvious neuroinflammation in CNS. TLR2 mediated depression-like behaviors and dysosmia induced by envelope protein, which could serve as a promising therapeutic target for neurological manifestation in COVID-19 patients.
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- 2023
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7. Separated Electron–Phonon and Phonon–Phonon Scatterings Across Interface in Thin Film LaCoO3/SrTiO3
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Wenjie Hao, Minghui Gu, Zhenyun Tian, Shaohua Fu, Meng Meng, Hong Zhang, Jiandong Guo, and Jimin Zhao
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coherent acoustic phonons ,electron–phonon coupling ,heterostructures ,phonon–phonon scattering ,ultrafast spectroscopy ,Science - Abstract
Abstract Electron–phonon coupling (EPC) and phonon–phonon scattering (PPS) are at the core of the microscopic physics mechanisms of vast quantum materials. However, to date, there are rarely reports that these two processes can be spatially separated, although they are usually temporally detached with different characteristic lifetimes. Here, by employing ultrafast spectroscopy to investigate the photo‐carrier ultrafast dynamics in a LaCoO3 thin film on a (100) SrTiO3 substrate, intriguing evidence is found that the two interactions are indeed spatially separated. The EPC mainly occurs in the thin film, whereas PPS is largely in the substrate, especially at the several atomic layers near the interface. Across‐interface penetration and decay of optical phonons into acoustic phonons thus naturally occur. An EPC strength λEg = 0.30 is also obtained and an acoustic phonon mode at 45.3 GHz is observed. The finding lays out a cornerstone for future quantum nano device designs.
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- 2024
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8. Altered frontoparietal activity in acoustic startle priming tasks during reticulospinal tract facilitation: An fNIRS study
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Nan Xia, Chang He, Xiupan Wei, Yang-An Li, Weiwei Lou, Minghui Gu, Zejian Chen, Jiang Xu, Yali Liu, Xiaohua Han, and Xiaolin Huang
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acoustic startle ,functional near-infrared spectroscopy ,reticulospinal tract ,rehabilitation ,frontoparietal cortex ,Neurosciences. Biological psychiatry. Neuropsychiatry ,RC321-571 - Abstract
BackgroundBecause it is one of the important pathways for promoting motor recovery after cortical injury, the function of the reticulospinal tract (RST) has received increasing attention in recent years. However, the central regulatory mechanism of RST facilitation and reduction of apparent response time is not well understood.ObjectivesTo explore the potential role of RST facilitation in the acoustic startle priming (ASP) paradigm and observe the cortical changes induced by ASP reaching tasks.MethodsTwenty healthy participants were included in this study. The reaching tasks were performed with their left and right hands. Participants were instructed to get ready after the warning cue and complete the reach as soon as they heard the Go cue. Half of the testing trials were set as control trials with an 80-dB Go cue. The other half of the trials had the Go cue replaced with 114-dB white noise to evoke the StartleReact effect, inducing reticulospinal tract facilitation. The response of the bilateral sternocleidomastoid muscle (SCM) and the anterior deltoid was recorded via surface electromyography. Startle trials were labeled as exhibiting a positive or negative StartleReact effect, according to whether the SCM was activated early (30–130 ms after the Go cue) or late, respectively. Functional near-infrared spectroscopy was used to synchronously record the oxyhemoglobin and deoxyhemoglobin fluctuations in bilateral motor-related cortical regions. The β values representing cortical responses were estimated via the statistical parametric mapping technique and included in the final analyses.ResultsSeparate analyses of data from movements of the left or right side revealed significant activation of the right dorsolateral prefrontal cortex during RST facilitation. Moreover, left frontopolar cortex activation was greater in positive startle trials than in control or negative startle trials during left-side movements. Furthermore, decreased activity of the ipsilateral primary motor cortex in positive startle trials during ASP reaching tasks was observed.ConclusionThe right dorsolateral prefrontal cortex and the frontoparietal network to which it belongs may be the regulatory center for the StartleReact effect and RST facilitation. In addition, the ascending reticular activating system may be involved. The decreased activity of the ipsilateral primary motor cortex suggests enhanced inhibition of the non-moving side during the ASP reaching task. These findings provide further insight into the SE and into RST facilitation.
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- 2023
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9. Limited value of serum neutrophil-to-lymphocyte ratio in the diagnosis of chronic periprosthetic joint infection
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Yongyu Ye, Weishen Chen, Minghui Gu, Qiaoli Liu, Guoyan Xian, Baiqi Pan, Linli Zheng, Xiaoling Chen, Ziji Zhang, and Puyi Sheng
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Chronic periprosthetic joint infection ,Neutrophil-to-lymphocyte ratio ,Diagnosis ,Orthopedic surgery ,RD701-811 - Abstract
Abstract Background Diagnosing chronic periprosthetic joint infection (PJI) is challenging. No single biomarker can accurately recognize PJI preoperatively in a timely manner. Therefore, the aim of the present study was to investigate the usefulness of the serum neutrophil-to-lymphocyte ratio (NLR) in aiding the diagnosis of chronic PJI. Materials and methods We retrospectively evaluated the medical records of 158 patients who had undergone revision arthroplasty (104 with aseptic mechanic failure and 54 with chronic PJI) from July 2011 to July 2020. Univariate analysis followed by multivariate logistic regression was applied to compare NLR, C-reactive protein (CRP), and erythrocyte sedimentation ratio (ESR) between the two groups. The receiver operating characteristic (ROC) curve was used to assess the diagnostic performance of NLR alone and in combination with CRP and ESR. Results NLR, CRP, and ESR were significantly higher in patients with chronic PJI than in the aseptic revision group (p
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- 2021
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10. Immediate and short-term effects of continuous theta burst transcranial magnetic stimulation over contralesional premotor area on post-stroke spasticity in patients with severe hemiplegia: Study protocol for a randomized controlled trial
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Xiupan Wei, Nan Xia, Yang-An Li, Minghui Gu, Tongming Zhang, Wei Gao, and Yali Liu
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spasticity ,stroke ,premotor area ,theta burst stimulation (TBS) ,H reflex ,randomized controlled trial ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
BackgroundPost-stroke spasticity is an important complication that greatly affects survivors' functional prognosis and daily activities. Increasing evidence points to aberrant contralesional neuromodulation compensation after brain injury as a possible culprit for increased spasticity in patients with severe stroke. Hyperactivity of the contralesional premotor area (cPMA) was supposed to be highly correlated with this progression. This study aims to demonstrate the immediate and short-term efficacy of continuous theta-burst stimulation (cTBS) targeting cPMA on upper limb spasticity in severe subacute stroke patients.MethodsThis trial is a single-center, prospective, three-group randomized controlled trial. Forty-five eligible patients will be recruited and randomized into three groups: the sham-cTBS group (sham cTBS targeting contralesional PMA), the cTBS-cM1 group (cTBS targeting contralesional M1), and the cTBS-cPMA group (cTBS targeting contralesional PMA). All subjects will undergo comprehensive rehabilitation and the corresponding cTBS interventions once a day, five times a week for 4 weeks. Clinical scales, neurophysiological examinations, and neuroimaging will be used as evaluation tools in this study. As the primary outcome, clinical performance on muscle spasticity of elbow/wrist flexor/extensors and upper-limb motor function will be evaluated with the modified Ashworth scale and the Fugl-Meyer Assessment of Upper Extremity Scale, respectively. These scale scores will be collected at baseline, after 4 weeks of treatment, and at follow-up. The secondary outcomes were neurophysiological examinations and Neuroimaging. In neurophysiological examinations, motor evoked potentials, startle reflex, and H reflexes will be used to assess the excitability of the subject's motor cortex, reticulospinal pathway, and spinal motor neurons, respectively. Results of them will be recorded before and after the first cTBS treatment, at post-intervention (at 4 weeks), and at follow-up (at 8 weeks). Neuroimaging tests with diffusion tensor imaging for all participants will be evaluated at baseline and after the 4-week treatment.DiscussionBased on the latest research progress on post-stroke spasticity, we innovatively propose a new neuromodulation target for improving post-stroke spasticity via cTBS. We expected that cTBS targeting cPMA would have significant immediate and short-term effects on spasticity and related neural pathways. The effect of cTBS-cPMA may be better than that of cTBS via conventional cM1. The results of our study will provide robust support for the application of cTBS neuromodulation in post-stroke spasticity after a severe stroke.Clinical trial registrationThis trial was registered with chictr.org.cn on June 13, 2022 (protocol version). http://www.chictr.org.cn/showproj.aspx?proj=171759.
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- 2022
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11. Action observation treatment-based exoskeleton (AOT-EXO) for upper extremity after stroke: study protocol for a randomized controlled trial
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Zejian Chen, Nan Xia, Chang He, Minghui Gu, Jiang Xu, Xiaohua Han, and Xiaolin Huang
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Stroke ,Action observation treatment ,Exoskeleton ,Upper limb ,Functional magnetic resonance imaging ,Randomized controlled trial ,Medicine (General) ,R5-920 - Abstract
Abstract Background Stroke produces multiple symptoms, including sensory, motor, cognitive and psychological dysfunctions, among which motor deficit is the most common and is widely recognized as a major contributor to long-term functional disability. Robot-assisted training is effective in promoting upper extremity muscle strength and motor impairment recovery after stroke. Additionally, action observation treatment can enhance the effects of physical and occupational therapy by increasing neural activation. The AOT-EXO trial aims to investigate whether action observation treatment coupled with robot-assisted training could enhance motor circuit activation and improve upper extremity motor outcomes. Methods The AOT-EXO trial is a multicentre, prospective, three-group randomized controlled trial (RCT). We will screen and enrol 132 eligible patients in the trial implemented in the Department of Rehabilitation Medicine of Tongji Hospital, Optical Valley Branch of Tongji Hospital and Hubei Province Hospital of Integrated Chinese & Western Medicine in Wuhan, China. Prior to study participation, written informed consent will be obtained from eligible patients in accordance with the Declaration of Helsinki. The enrolled stroke patients will be randomized to three groups: the CT group (conventional therapy); EXO group (exoskeleton therapy) and AOT-EXO group (action observation treatment-based exoskeleton therapy). The patients will undergo blinded assessments at baseline, post-intervention (after 4 weeks) and follow-up (after 12 weeks). The primary outcome will be the Fugl-Meyer Assessment for Upper Extremity (FMA-UE). Secondary outcomes will include the Action Research Arm Test (ARAT), modified Barthel Index (MBI), kinematic metrics assessed by inertial measurement unit (IMU), resting motor threshold (rMT), motor evoked potentials (MEP), functional magnetic resonance imaging (fMRI) and safety outcomes. Discussion This trial will provide evidence regarding the feasibility and efficacy of the action observation treatment-based exoskeleton (AOT-EXO) for post-stroke upper extremity rehabilitation and elucidate the potential underlying kinematic and neurological mechanisms. Trial registration Chinese Clinical Trial Registry ChiCTR1900026656 . Registered on 17 October 2019.
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- 2021
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12. Tannic Acid Induces Intestinal Dysfunction and Intestinal Microbial Dysregulation in Brandt’s Voles (Lasiopodomys brandtii)
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Minghui Gu, Ruiyang Fan, Xin Dai, Chen Gu, Aiqin Wang, Wanhong Wei, and Shengmei Yang
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Brandt’s vole ,tannic acid ,intestinal function ,gut microbiota ,Veterinary medicine ,SF600-1100 ,Zoology ,QL1-991 - Abstract
Brandt’s vole (Lasiopodomys brandtii) is a small herbivorous mammal that feeds on plants rich in secondary metabolites (PSMs), including tannins. However, plant defense mechanisms against herbivory by Brandt’s voles are not clearly established. This study aimed to investigate the effects of dietary tannic acid (TA) on the growth performance, intestinal morphology, digestive enzyme activities, cecal fermentation, intestinal barrier function, and gut microbiota in Brandt’s voles. The results showed that TA significantly hindered body weight gain, reduced daily food intake, changed the intestinal morphology, reduced digestive enzyme activity, and increased the serum zonulin levels (p < 0.05). The number of intestinal goblet and mast cells and the levels of serum cytokines and immunoglobulins (IgA, IgG, TNF-α, IL-6, and duodenal SlgA) were all reduced by TA (p < 0.05). Moreover, TA altered β-diversity in the colonic microbial community (p < 0.05). In conclusion, the results indicate that TA could damage the intestinal function of Brandt’s voles by altering their intestinal morphology, decreasing digestive ability and intestinal barrier function, and altering microbiota composition. Our study investigated the effects of natural PSMs on the intestinal function of wildlife and improved our general understanding of plant–herbivore interactions and the ecological role of PSMs.
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- 2023
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13. Fluctuation of fasting blood glucose in patients who underwent primary or revision total joint arthroplasty: a retrospective review
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Yongyu Ye, Baiqi Pan, Minghui Gu, Guoyan Xian, Weishen Chen, Linli Zheng, Ziji Zhang, and Puyi Sheng
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Total joint arthroplasty ,Revision ,Fasting blood glucose ,Fluctuation ,Hyperglycemia ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Perioperative hyperglycemia is a risk factor for postoperative complications after total joint arthroplasty (TJA). However, the variability of fasting blood glucose (FBG) after TJA remains unknown. We aimed to assess the fluctuation and extent of elevation of FBG following primary or revision TJA. Methods We retrospectively evaluated the medical records of 1788 patients who underwent primary or revision TJA between 2013 and 2018. We examined FBG values collected during 6 days of the perioperative period. The findings for each time point were evaluated with descriptive statistics. Postoperative glycemic variability was assessed by the coefficient of variation (CV). Results The final cohort included the medical records of 1480 patients (1417 primary and 63 revision). FBG was highest on postoperative day 1 in the primary and revision groups (P < 0.001), which had the highest number of hyperglycemic patients (FBG > 100 mg/dL), with 66.4% and 75.5% in the primary and revision groups, respectively. The CV of diabetics in the primary group, and diabetics and non-diabetics in the revision group, was higher than that of non-diabetics in the primary group. Conclusion Postoperative day 1 showed the highest FBG levels and proportion of patients with hyperglycemia in the perioperative period. Primary group diabetics, and revision group diabetics and non-diabetics, had higher postoperative fluctuation of FBG than primary group non-diabetics. Frequent FBG monitoring may therefore be warranted in diabetic patients undergoing TJA, and all patients undergoing revision TJA.
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- 2020
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14. SPHK Inhibitors and Zoledronic Acid Suppress Osteoclastogenesis and Wear Particle-Induced Osteolysis
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Minghui Gu, Baiqi Pan, Weishen Chen, Hai Xu, Xiaoyu Wu, Xuantao Hu, Linli Zheng, Yongyu Ye, Qing Meng, Guoyan Xian, Ziji Zhang, and Puyi Sheng
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SPHKs ,osteoclast ,aseptic prosthetic loosening ,periprosthetic osteolysis ,inflammation ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Background: Inflammatory osteolysis induced by wear particles is the major cause of prosthetic loosening after artificial joint replacement, and its prevention and treatment are difficult worldwide. Our previous study confirmed that sphingosine kinases (SPHKs) are important mediators regulating the wear particle-induced macrophage inflammatory response. However, it is unclear whether SPHKs can modulate chronic inflammation and alleviate osteolysis. Zoledronic acid (ZA), an imidazole-containing bisphosphonate, directly affects osteoclasts and prevents bone mineral-related diseases. However, the effects of SPHK inhibitors and ZA used to treat periprosthetic osteolysis are unknown.Methods: We applied tartrate-resistant acid phosphatase (TRAP) staining to evaluate bone destruction in the interface membranes of patients with aseptic loosening and a control group. A murine calvarial osteolysis model was used to examine the preventative effect of SPHK inhibitors and ZA on osteolysis. Micro-CT scanning, immunohistochemistry (IHC), and histomorphometric analysis were conducted to determine the variations in inflammatory osteolysis. The effects of different drug concentrations on cell viability were evaluated using the Cell Counting Kit-8 (CCK-8) assay. Real-time quantitative polymerase chain reaction (RT-qPCR) analysis was performed to confirm the reduced expression of osteoclast-specific genes after drug and titanium treatment. The osteoclast formation and functions of the drugs were analyzed using TRAP staining in vivo and in vitro. The effect of SPHKs/S1P-TRAF2-BECN1 signaling pathways was verified via RT-qPCR and tissue IHC.Results: In this study, we found that SPHK inhibitors (ABC294640 and FTY720) combined with ZA decreased the degree of inflammatory osteolysis in vivo. However, ABC294640 and ZA suppressed osteoclast differentiation and osteoclast-specific genes in vitro. SPHKs regulate the inflammatory osteolysis induced by wear particles by increasing the expression of SPHKs/S1P-TRAF2-BECN1.Conclusion: Our study revealed that wear particles could induce inflammatory osteolysis by upregulating SPHKs/S1P-TRAF2-BECN1 and SPHK inhibitors/ZA inhibit osteoclastogenesis in vitro and prevent inflammatory osteolysis in vivo, suggesting that SPHK inhibitors and ZA can be a new perspective and scientific basis for the prevention and treatment of prosthesis loosening.
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- 2022
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15. Serum globulin and albumin to globulin ratio as potential diagnostic biomarkers for periprosthetic joint infection: a retrospective review
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Yongyu Ye, Weishen Chen, Minghui Gu, Guoyan Xian, Baiqi Pan, Linli Zheng, Ziji Zhang, and Puyi Sheng
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Periprosthetic joint infection ,Globulin ,Albumin to globulin ratio ,Diagnosis ,Orthopedic surgery ,RD701-811 ,Diseases of the musculoskeletal system ,RC925-935 - Abstract
Abstract Background Periprosthetic joint infection (PJI) has been increasingly documented; however, its preoperative accurate diagnosis remains challenging. Furthermore, there is a dire need to identify appropriate and effective biomarkers. We aimed to evaluate the relationship between globulin, albumin to globulin (A/G) ratio, and development of PJI in patients undergoing revision total joint arthroplasty (TJA). Methods A retrospective study was conducted on patients who had undergone revision TJA between 2011 and 2018 (89 with aseptic mechanic failure and 38 with PJI). The serum proteins were explored using univariate analysis followed by multivariate logistic regression. The diagnostic performance of these proteins was assessed by the receiver operating characteristic (ROC) curve. Results Higher globulin levels (odds ratio [OR], 1.239; P < 0.001) and lower A/G ratio (OR, 0.007; P < 0.001) were strongly associated with the risk of PJI. ROC curve analysis demonstrated reasonable diagnostic performance for globulin (area under the curve [AUC], 0.77; sensitivity, 78.95%; and specificity, 69.66%) and A/G ratio (AUC, 0.779; sensitivity, 65.79%; and specificity, 78.65%). Conclusions Both globulin and A/G ratio were associated with PJI and may serve as potential adjuvant biomarkers in the diagnosis of PJI.
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- 2020
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16. Startle Increases the Incidence of Anticipatory Muscle Activations but Does Not Change the Task-Specific Muscle Onset for Patients After Subacute Stroke
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Nan Xia, Chang He, Yang-An Li, Minghui Gu, Zejian Chen, Xiupan Wei, Jiang Xu, and Xiaolin Huang
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startle ,stroke ,anticipatory muscle activation ,rehabilitation ,anticipatory postural adjustments ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Objectives: To demonstrate the task-specificities of anticipatory muscle activations (AMAs) among different forward-reaching tasks and to explore the StartleReact Effect (SE) on AMAs in occurrence proportions, AMA onset latency or amplitude within these tasks in both healthy and stroke population.Methods: Ten healthy and ten stroke subjects were recruited. Participants were asked to complete the three forward-reaching tasks (reaching, reaching to grasp a ball or cup) on the left and right hand, respectively, with two different starting signals (warning-Go, 80 dB and warning-startle, 114 dB). The surface electromyography of anterior deltoid (AD), flexor carpi radialis (FCR), and extensor carpi radialis (ECR) on the moving side was recorded together with signals from bilateral sternocleidomastoid muscles (SCM), lower trapezius (LT), latissimus dorsi (LD), and tibialis anterior (TA). Proportions of valid trials, the incidence of SE, AMA incidence of each muscle, and their onset latency and amplitude were involved in analyses. The differences of these variables across different move sides (healthy, non-paretic, and paretic), normal or startle conditions, and the three tasks were explored. The ECR AMA onset was selected to further explore the SE on the incidence of AMAs.Results: Comparisons between move sides revealed a widespread AMA dysfunction in subacute stroke survivors, which was manifested as lower AMA onset incidence, changed onset latency, and smaller amplitude of AMAs in bilateral muscles. However, a significant effect of different tasks was only observed in AMA onset latency of muscle ECR (F = 3.56, p = 0.03, η2p = 0.011), but the significance disappeared in the subsequent analysis of the stroke subjects only (p > 0.05). Moreover, the following post-hoc comparison indicated significant early AMA onsets of ECR in task cup when comparing with reach (p < 0.01). For different stimuli conditions, a significance was only revealed on shortened premotor reaction time under startle for all participants (F = 60.68, p < 0.001, ηp2 = 0.056). Furthermore, stroke survivors had a significantly lower incidence of SE than healthy subjects under startle (p < 0.01). But all performed a higher incidence of ECR AMA onset (p < 0.05) than with normal signal. In addition, the incidence of ECR AMAs of both non-paretic and paretic sides could be increased significantly via startle (p ≤ 0.02).Conclusions: Healthy people have task-specific AMAs of muscle ECR when they perform forward-reaching tasks with different hand manipulations. However, this task-specific adjustment is lost in subacute stroke survivors. SE can improve the incidence of AMAs for all subjects in the forward-reaching tasks involving precision manipulations, but not change AMA onset latency and amplitude.
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- 2022
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17. Electroacupuncture Reduces Oocyte Number and Maintains Vascular Barrier Against Ovarian Hyperstimulation Syndrome by Regulating CD200
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Li Chen, Xuan Huang, Li Wang, Cencen Wang, Xu Tang, Minghui Gu, Jun Jing, Rujun Ma, Xie Ge, and Bing Yao
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CD200 ,anti-inflammation ,vascular barrier ,electroacupuncture ,OHSS ,Biology (General) ,QH301-705.5 - Abstract
Ovarian hyperstimulation syndrome (OHSS) is a common complication caused by ovulatory stimulation therapy, which manifests as an increase in ovarian volume, an increase in the number of oocytes retrieved, and increased vascular permeability throughout the body and especially in ovarian tissue. In our previous study, we found that electroacupuncture (EA) could prevent the progression of OHSS, by mainly affecting ovary. However, the specific molecules and the mechanism of this process were still unknown. In order to explore the underlying mechanism, OHSS rat model was established and EA treatment was performed, which was followed by proteomic analysis of ovaries. Results showed a significant increase in the expression level of CD200 in the ovaries of OHSS group treated with EA than those of OHSS group. Clinical data showed that the level of CD200 in follicular fluid was negatively correlated with the number of oocytes retrieved and serum E2 level. Further in vitro experiments showed a concentration-dependent role of human chorionic gonadotropin (hCG) in reducing CD200 and CD200R levels, and increasing inflammatory cytokine levels in cultured KGN cells. In human umbilical vein endothelial cells (HUVECs), the vascular barrier function was improved by CM (cultural medium from KGN cell) which treated with CD200Fc (CD200R agonist). Meanwhile, the results of in vivo experiments indicated that EA reduced the number of ovarian corpora lutea, decreased inflammatory response, and improved the vascular barrier function by increasing the expression of CD200 and CD200R in rat ovaries. These findings suggest that EA treatment may reduce oocyte number and maintain vascular barrier against OHSS through ovarian anti-inflammatory response mediated by CD200. Therefore, this study is the first to identify CD200 as a main of EA in the ovary and elucidate the possible mechanism of EA on preventing and treating OHSS, which provide a scientific basis for CD200 as an effector and indicator in EA treatment.
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- 2021
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18. Novel method of boundary-free mesh parameterization.
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Liming Duan, Xueqing Luo, Lang Ruan, and Minghui Gu
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Medicine ,Science - Abstract
Unless the targeted mesh is developable, metric distortion is inevitable during the process of surface mesh parameterization, thus one important objective of all involved parametric studies is to reduce the metric distortion. In order to further reduce area and angle distortion, a novel method of boundary-free mesh parameterization is presented in the paper. Firstly, the initial boundary-fixed conformal parameterization from 3D surface mesh patch to a plane is performed in the method. Then, based on the initial parameterization, the iterations of boundary-free quasi-harmonic parameterization are developed, where the tensor field is updated in each iterative step and the principal curvature direction is utilized to terminate the iteration. The solution of the novel method is convenient to calculate since it involves a series of linear systems. In our novel parameterization method, lower metric distortion and considerable efficiency have been obtained in experiments.
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- 2019
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19. Direct Growth of Antimonene on C-Plane Sapphire by Molecular Beam Epitaxy
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Minghui Gu, Chen Li, Yuanfeng Ding, Kedong Zhang, Shunji Xia, Yusheng Wang, Ming-Hui Lu, Hong Lu, and Yan-Feng Chen
- Subjects
antimonene ,molecular beam epitaxy ,effective adsorption ,sapphire ,two dimensional materials ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Monolayer antimony (antimonene) has been reported for its excellent properties, such as tuneable band gap, stability in the air, and high mobility. However, growing high quality, especially large-area antimonene, remains challenging. In this study, we report the direct growth of antimonene on c-plane sapphire substrate while using molecular beam epitaxy (MBE). We explore the effect of growth temperature on antimonene formation and present a growth phase diagram of antimony. The effect of antimony sources (Sb2 or Sb4) and a competing mechanism between the two-dimensional (2D) and three-dimensional (3D) growth processes and the effects of adsorption and cracking of the source molecules are also discussed. This work offers a new method for growing antimonene and it provides ideas for promoting van der Waals epitaxy.
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- 2020
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20. A shape-based inter-layer contours correspondence method for ICT-based reverse engineering.
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Liming Duan, Shangpeng Yang, Gui Zhang, Fei Feng, and Minghui Gu
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Medicine ,Science - Abstract
The correspondence of a stack of planar contours in ICT (industrial computed tomography)-based reverse engineering, a key step in surface reconstruction, is difficult when the contours or topology of the object are complex. Given the regularity of industrial parts and similarity of the inter-layer contours, a specialized shape-based inter-layer contours correspondence method for ICT-based reverse engineering was presented to solve the above problem based on the vectorized contours. In this paper, the vectorized contours extracted from the slices consist of three graphical primitives: circles, arcs and segments. First, the correspondence of the inter-layer primitives is conducted based on the characteristics of the primitives. Second, based on the corresponded primitives, the inter-layer contours correspond with each other using the proximity rules and exhaustive search. The proposed method can make full use of the shape information to handle industrial parts with complex structures. The feasibility and superiority of this method have been demonstrated via the related experiments. This method can play an instructive role in practice and provide a reference for the related research.
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- 2017
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21. An Improved Approach of Mesh Segmentation to Extract Feature Regions.
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Minghui Gu, Liming Duan, Maolin Wang, Yang Bai, Hui Shao, Haoyu Wang, and Fenglin Liu
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Medicine ,Science - Abstract
The objective of this paper is to extract concave and convex feature regions via segmenting surface mesh of a mechanical part whose surface geometry exhibits drastic variations and concave-convex features are equally important when modeling. Referring to the original approach based on the minima rule (MR) in cognitive science, we have created a revised minima rule (RMR) and presented an improved approach based on RMR in the paper. Using the logarithmic function in terms of the minimum curvatures that are normalized by the expectation and the standard deviation on the vertices of the mesh, we determined the solution formulas for the feature vertices according to RMR. Because only a small range of the threshold parameters was selected from in the determined formulas, an iterative process was implemented to realize the automatic selection of thresholds. Finally according to the obtained feature vertices, the feature edges and facets were obtained by growing neighbors. The improved approach overcomes the inherent inadequacies of the original approach for our objective in the paper, realizes full automation without setting parameters, and obtains better results compared with the latest conventional approaches. We demonstrated the feasibility and superiority of our approach by performing certain experimental comparisons.
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- 2015
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22. Support Effect and Stability of Inverse Fe1(OH)x/Pt Single-Site Interfacial Structured Catalysts for Preferential CO Oxidation in H2
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Minghui Gu, Lina Cao, Hui Ye, and Junling Lu
- Subjects
General Energy ,Physical and Theoretical Chemistry ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Published
- 2022
23. The Measurement and Spatial-Temporal Distribution of the Development Level of China's Digital Economy
- Author
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Minghui, Gu, primary, Zhaoyan, Chen, additional, and Hui, Chen, additional
- Published
- 2023
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24. Modulation of the Metal–Nonmetal Crossover in SrIrO3/CaMnO3 Superlattices
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Minghui Gu, Ruixue Zhu, Xinxin Zhang, Zhenzhen Wang, Qichang An, Fang Yang, Xiaoran Liu, Peng Gao, Meng Meng, and Jiandong Guo
- Subjects
Materials Chemistry ,Electrochemistry ,Electronic, Optical and Magnetic Materials - Published
- 2022
25. Movements Classification Through sEMG With Convolutional Vision Transformer and Stacking Ensemble Learning
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Shu Shen, Xuebin Wang, Fan Mao, Lijuan Sun, and Minghui Gu
- Subjects
Electrical and Electronic Engineering ,Instrumentation - Published
- 2022
26. Identification of anterior cruciate ligament fibroblasts and their contribution for knee osteoarthritis progression by single-cell analyses
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Ziji Zhang, Zhiwen Li, Shiyong Zhang, Yiyang Xu, Guping Mao, Yunze Kang, Linli Zheng, Dianbo Long, Weishen Chen, Minghui Gu, Zhiqi Zhang, Yan Kang, and Puyi Sheng
- Abstract
Objective A better Understanding of the key regulatory cells in the anterior cruciate ligament (ACL), and their role and regulatory mechanisms in knee osteoarthritis (KOA) progression can facilitate the development of targeted treatment strategies for KOA. Methods The relationship between ACL degeneration and KOA was first explored using human ACL specimens and mouse models. Next, single-cell RNA sequencing (scRNA-seq) and single-cell detection of transposase accessible and chromatin sequencing (scATAC-seq) data were integrated to reveal the transcriptional and epigenomic landscape of ACL in normal and osteoarthritis (OA) states. Results Six cell populations were identified in the human ACL, among which were inflammation-associated fibroblasts (IAFs). Degeneration of the ACL during OA mechanically alters the knee joint homeostasis and influences the microenvironment by regulating inflammatory- and osteogenic-related factors, thereby contributing to the progression of KOA. Specifically, a IAF subpopulation identified in OA ACL was found to enhance the transcription and secretion of EGER via SOX5 upregulation, with consequent activation of the EGER–EGFR signaling pathway. These molecular events led to the upregulation of downstream inflammatory and osteogenic factors, and the downregulation of the extracellular matrix-associated factor, thereby leading to knee osteoid formation, cartilage degeneration, and OA progression. Conclusions In summary, this study identifies a novel subpopulation of fibroblasts in the ACL, which confirms the importance of the ACL in knee joint homeostasis and disease. Additionally, the specific mechanism by which these IAFs regulate KOA progression was uncovered, which provides new foundation for the development of targeted treatments for KOA.
- Published
- 2023
27. Insights from proteome to phosphorylated proteome: deciphering different regulatory mechanisms in goat muscles with high‐ and low‐meat quality
- Author
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Minghui Gu, Yanchao Wei, Yang Jiao, Dequan Zhang, and Yongfeng Liu
- Subjects
Industrial and Manufacturing Engineering ,Food Science - Published
- 2022
28. Changes in transcriptome of goat muscle during frozen, ice‐temperature and chilled storage within 7 days
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Yu Xiao, Qian Shen, Minghui Gu, Yang Jiao, and Yongfeng Liu
- Subjects
Industrial and Manufacturing Engineering ,Food Science - Published
- 2022
29. Insight from untargeted metabolomics: Revealing the potential marker compounds changes in refrigerated pork based on random forests machine learning algorithm
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Minghui Gu, Cheng Li, Li Chen, Shaobo Li, Naiyu Xiao, Dequan Zhang, and Xiaochun Zheng
- Subjects
General Medicine ,Food Science ,Analytical Chemistry - Published
- 2023
30. Transformable 3D curved high-density liquid metal coils – an integrated unit for general soft actuation, sensing and communication
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Nan Li, Yingxin Zhou, Yuqing Li, Chunwei Li, Wentao Xiang, Xueqing Chen, Pan Zhang, Qi Zhang, Jun Su, Bohao Jin, Huize Song, Cai Cheng, Minghui Guo, Lei Wang, and Jing Liu
- Subjects
Science - Abstract
Abstract Rigid solenoid coils have long been indispensable in modern intelligent devices. However, their sparse structure and challenging preparation of flexible coils for soft robots impose limitations. Here, a transformable 3D curved high-density liquid metal coil (HD-LMC) is introduced that surpasses the structural density level of enameled wire. The fabrication technique employed for high-density channels in elastomers is universally applicable. Such HD-LMCs demonstrated excellent performance in pressure, temperature, non-contact distance sensors, and near-field communication. Soft electromagnetic actuators thus achieved significantly improved the electromagnetic force and power density. Moreover, precise control of swinging tail motion enables a bionic pufferfish to swim. Finally, HD-LMC is further utilized to successfully implement a soft rotary robot with integrated sensing and actuation capabilities. This groundbreaking research provides a theoretical and experimental basis for expanding the applications of liquid metal-based multi-dimensional complex flexible electronics and is expected to be widely used in liquid metal-integrated robotic systems.
- Published
- 2024
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31. SARS-CoV-2 Envelope protein triggers depression and dysosmia via TLR2 mediated neuroinflammation
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Wenliang Su, Jiahang Ju, Minghui Gu, Xinrui Wang, Shaozhuang Liu, Jiawen Yu, and Dongliang Mu
- Abstract
BackgroundDepression and dysosmia have been regarded as the main neurological symptoms in COVID-19 patients, the mechanism of which remains unclear. Current studies have demonstrated that SARS-CoV-2 envelope protein served as a pro-inflammatory factor as sensed by Toll like receptor 2 (TLR2), suggesting the viral infection independent pathological feature of E protein. In this study, we aim to determine the role of E protein in depression, dysosmia and associated neuroinflammation in central nervous system (CNS).MethodsDepression and olfactory function were observed in both female and male mice as receiving intracisternal injection of envelope protein. Immunohistochemistry was applied in conjunction with RT-PCR to assess the glial activation, blood-brain barrier status and mediators synthesis in cortex, hippocampus and olfactory bulb. TLR2 was pharmacologically blocked to determine its role in E protein related depression and dysosmia.ResultsIntracisternal injection of envelope protein evoked depression and dysosmia in both female and male mice. Immunohistochemistry suggested that envelope protein upregulated IBA1 and GFAP in cortex, hippocampus and olfactory bulb, while ZO-1 was downregulated. Moreover, IL-1β, TNF-α, IL-6, CCL2, MMP2 and CSF1 were upregulated in both cortex and hippocampus, whereas IL-1β, IL-6 and CCL2 were upregulated in olfactory bulb. Furtherly, inhibiting microglia, but not astrocyte, alleviated depression and dysosmia induced by envelope protein. Finally, RT-PCR and immunohistochemistry suggested that TLR2 was upregulated in cortex, hippocampus and olfactory bulb, the blocking of which mitigated depression and dysosmia induced by envelope protein.ConclusionsOur study demonstrates that envelope protein could directly induce depression and dysosmia together with obvious neuroinflammation in CNS. TLR2 mediated depression and dysosmia induced by envelope protein, which could serve as a promising therapeutic target for neurological manifestation in COVID-19 patients.
- Published
- 2023
32. Novel insights from protein degradation: Deciphering the dynamic evolution of biogenic amines as a quality indicator in pork during storage
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Minghui Gu, Cheng Li, Yuanyuan Su, Li Chen, Shaobo Li, Xin Li, Xiaochun Zheng, and Dequan Zhang
- Subjects
Food Science - Published
- 2023
33. Fluctuation of fasting blood glucose in patients who underwent primary or revision total joint arthroplasty: a retrospective review
- Author
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Ziji Zhang, Linli Zheng, Puyi Sheng, Minghui Gu, Yongyu Ye, Weishen Chen, Baiqi Pan, and Guoyan Xian
- Subjects
Blood Glucose ,Male ,Reoperation ,medicine.medical_specialty ,Joint arthroplasty ,Time Factors ,lcsh:Diseases of the musculoskeletal system ,Revision ,Arthroplasty, Replacement, Hip ,03 medical and health sciences ,0302 clinical medicine ,Postoperative Complications ,lcsh:Orthopedic surgery ,Risk Factors ,medicine ,Humans ,Orthopedics and Sports Medicine ,In patient ,030212 general & internal medicine ,Postoperative Period ,Risk factor ,Arthroplasty, Replacement, Knee ,Perioperative Period ,Glycemic ,Aged ,Retrospective Studies ,030222 orthopedics ,business.industry ,Medical record ,Perioperative ,Fasting ,Fluctuation ,Middle Aged ,Surgery ,lcsh:RD701-811 ,Hyperglycemia ,Orthopedic surgery ,Cohort ,Fasting blood glucose ,Total joint arthroplasty ,Female ,lcsh:RC925-935 ,business ,Biomarkers ,Research Article - Abstract
Background Perioperative hyperglycemia is a risk factor for postoperative complications after total joint arthroplasty (TJA). However, the variability of fasting blood glucose (FBG) after TJA remains unknown. We aimed to assess the fluctuation and extent of elevation of FBG following primary or revision TJA. Methods We retrospectively evaluated the medical records of 1788 patients who underwent primary or revision TJA between 2013 and 2018. We examined FBG values collected during 6 days of the perioperative period. The findings for each time point were evaluated with descriptive statistics. Postoperative glycemic variability was assessed by the coefficient of variation (CV). Results The final cohort included the medical records of 1480 patients (1417 primary and 63 revision). FBG was highest on postoperative day 1 in the primary and revision groups (P < 0.001), which had the highest number of hyperglycemic patients (FBG > 100 mg/dL), with 66.4% and 75.5% in the primary and revision groups, respectively. The CV of diabetics in the primary group, and diabetics and non-diabetics in the revision group, was higher than that of non-diabetics in the primary group. Conclusion Postoperative day 1 showed the highest FBG levels and proportion of patients with hyperglycemia in the perioperative period. Primary group diabetics, and revision group diabetics and non-diabetics, had higher postoperative fluctuation of FBG than primary group non-diabetics. Frequent FBG monitoring may therefore be warranted in diabetic patients undergoing TJA, and all patients undergoing revision TJA.
- Published
- 2020
34. The Impact of Parental Involvement in Creating a Positive Learning Environment for Elementary School Students.
- Author
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Minghui Gu and Jie Zhang
- Subjects
- *
PARENTING , *CLASSROOM environment , *SCHOOL environment , *SCHOOL children , *PARENT-teacher cooperation , *SCHOOL involvement , *CONCEPT mapping - Abstract
This paper explores how parents and teachers view and do parental involvement in elementary schools. It uses surveys and interviews with parents and teachers from two schools. It finds that both groups value parental involvement and its positive effects on students' learning and happiness. But it also identifies some problems that prevent good parental involvement, like time, communication, resources, and training. It suggests some ways to improve parental involvement and cooperation between parents and teachers. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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35. CCL3/CCR1 mediates CD14+CD16− circulating monocyte recruitment in knee osteoarthritis progression
- Author
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Xiaonan Xu, Fangang Meng, Xiaoyi Zhao, Minghui Gu, Xingzhao Wen, Lin Li, Guangpu Yang, and Puyi Sheng
- Subjects
030203 arthritis & rheumatology ,0301 basic medicine ,CCR1 ,CCR2 ,Chemokine ,biology ,Monocyte chemotaxis ,business.industry ,CD14 ,Monocyte ,Biomedical Engineering ,hemic and immune systems ,03 medical and health sciences ,Chemokine receptor ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,Rheumatology ,parasitic diseases ,Immunology ,biology.protein ,Synovial fluid ,Medicine ,Orthopedics and Sports Medicine ,business - Abstract
Summary Objectives Monocyte-derived macrophages, as the predominant immune cell type that is increased in inflamed synovium, play a vital role during knee osteoarthritis (KOA) progression. However, the mechanisms underlying the recruitment of circulating monocytes to osteoarthritic knees remain uncertain. Based on previous data obtained from plasma, we investigated the contributions of CCL2, CCL3, CCL4 and their cognate receptors in circulating monocyte chemotaxis and KOA development. Methods Using flow cytometry staining, we characterized the expression patterns of the chemokine receptors in CD14+CD16− circulating monocytes from KOA patients and healthy volunteers. The expression of chemokines in synovial fluids, synovium and cartilage was investigated in KOA patients and in patients without KOA. The role of chemokines and their cognate receptors in the chemotaxis of CD14+CD16− circulating monocytes was assessed using chemokine neutralizing antibodies (NA) and receptor antagonists in vitro and in vivo. Results The majority of CD14+CD16− circulating monocytes were CCR1-and CCR2-positive. CCL2, CCL3 and CCL4 were elevated in synovial fluid of KOA patients compared with that of controls. The most likely source of these chemokines is inflamed synovium and cartilage in the osteoarthritic knee. The CCL3/CCR1 and CCL2/CCR2 axes showed substantial ability to recruit CD14+CD16− monocytes in transwell assays. Similar results were confirmed in a mouse model of collagenase-induced KOA (CIA) in which blocking either the CCL3/CCR1 axis or the CCL2/CCR2 axis reduced synovial hyperplasia and F4/80+ macrophage infiltration. Conclusions Our findings suggested that, analogous to the CCL2/CCR2 axis, CCL3 produced in osteoarthritic knees can chemoattract circulating monocytes to the inflamed synovium through CCR1.
- Published
- 2020
36. Author response for 'Insights from proteome to phosphorylated proteome: deciphering different regulatory mechanisms in goat muscles with high‐ and low‐meat quality'
- Author
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null Minghui Gu, null Yanchao Wei, null Yang Jiao, null Dequan Zhang, and null Yongfeng Liu
- Published
- 2022
37. Single-cell RNA-seq analysis identifies meniscus progenitors and reveals the progression of meniscus degeneration
- Author
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Weiming Liao, Peihui Wu, Xiaoyi Zhao, Shu Hu, Ziji Zhang, Hongyi Li, Zhiqi Zhang, Guping Mao, Ruofan Ma, Hao Sun, Minghui Gu, and Xingzhao Wen
- Subjects
0301 basic medicine ,Immunology ,Cell ,Meniscus (anatomy) ,General Biochemistry, Genetics and Molecular Biology ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Rheumatology ,Tissue engineering ,fibroblasts ,Osteoarthritis ,Animals ,Humans ,Immunology and Allergy ,Medicine ,Meniscus ,RNA-Seq ,Progenitor cell ,Gene ,Progenitor ,030203 arthritis & rheumatology ,Sequence Analysis, RNA ,business.industry ,Stem Cells ,Endothelial Cells ,RNA ,Cell Differentiation ,musculoskeletal system ,cytokines ,Cell biology ,body regions ,030104 developmental biology ,medicine.anatomical_structure ,arthritis ,Disease Progression ,CD146 ,Single-Cell Analysis ,business - Abstract
ObjectivesThe heterogeneity of meniscus cells and the mechanism of meniscus degeneration is not well understood. Here, single-cell RNA sequencing (scRNA-seq) was used to identify various meniscus cell subsets and investigate the mechanism of meniscus degeneration.MethodsscRNA-seq was used to identify cell subsets and their gene signatures in healthy human and degenerated meniscus cells to determine their differentiation relationships and characterise the diversity within specific cell types. Colony-forming, multi-differentiation assays and a mice meniscus injury model were used to identify meniscus progenitor cells. We investigated the role of degenerated meniscus progenitor (DegP) cell clusters during meniscus degeneration using computational analysis and experimental verification.ResultsWe identified seven clusters in healthy human meniscus, including five empirically defined populations and two novel populations. Pseudotime analysis showed endothelial cells and fibrochondrocyte progenitors (FCP) existed at the pseudospace trajectory start. Melanoma cell adhesion molecule ((MCAM)/CD146) was highly expressed in two clusters. CD146+ meniscus cells differentiated into osteoblasts and adipocytes and formed colonies. We identified changes in the proportions of degenerated meniscus cell clusters and found a cluster specific to degenerative meniscus with progenitor cell characteristics. The reconstruction of four progenitor cell clusters indicated that FCP differentiation into DegP was an aberrant process. Interleukin 1β stimulation in healthy human meniscus cells increased CD318+ cells, while TGFβ1 attenuated the increase in CD318+ cells in degenerated meniscus cells.ConclusionsThe identification of meniscus progenitor cells provided new insights into cell-based meniscus tissue engineering, demonstrating a novel mechanism of meniscus degeneration, which contributes to the development of a novel therapeutic strategy.
- Published
- 2019
38. Hyaluronan size alters chondrogenesis of mesenchymal stem cells cultured on tricalcium phosphate-collagen-hyaluronan scaffolds
- Author
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Yueran Xue, Anyu Zeng, Fangang Meng, Dianbo Long, Xiaoyi Zhao, Mei Shang, Xingzhao Wen, Minghui Gu, Aishan He, and Zibo Yang
- Subjects
Calcium Phosphates ,Materials science ,Biomedical Engineering ,Biomaterials ,Mice ,Nude mouse ,In vivo ,medicine ,Animals ,Humans ,Hyaluronic Acid ,Cells, Cultured ,biology ,Tissue Engineering ,Tissue Scaffolds ,Cartilage ,Regeneration (biology) ,Mesenchymal stem cell ,CD44 ,Metals and Alloys ,Cell Differentiation ,Mesenchymal Stem Cells ,equipment and supplies ,Chondrogenesis ,biology.organism_classification ,In vitro ,Cell biology ,medicine.anatomical_structure ,Ceramics and Composites ,biology.protein ,Collagen ,Rabbits - Abstract
Hyaluronan (HA) provides a favorable environment for chondrogenesis of bone marrow mesenchymal stem cells (BMSCs). A previous report from our group indicated that addition of HA increases the chondro-inductive capacity of scaffolds. Therefore, this study aimed to investigate whether the Mw of the HA could affect chondrogenesis of BMSCs seeded on TCP-COL-HA scaffolds. Human BMSCs (hBMSCs) and rabbit BMSCs (rBMSCs) were isolated and expanded. TCP-COL scaffolds and TCP-COL-HA scaffolds with two different HA Mws were assessed for their capacity to induce cartilage regeneration from hBMSCs in vitro and in vivo. The results showed that about 96.96% of hBMSCs expressed CD44. Moreover, Hyal-1 and chondrogenic marker genes expressions were increased in hMSCs seeded on TCP-COL-HA scaffolds, and blocking the HA-CD44 interaction with an anti-CD44 antibody reduced the expression levels of Hyal-1 and chondrogenic marker genes. Additionally, TCP-COL-HA scaffolds with 2000 kDa Mw showed greater induction of BMSC chondrogenesis induction compared with those with 80 kDa Mw. Similar results were observed in an ectopic implantation nude mouse model. In a rabbit osteochondral defect repair model, rBMSCs seeded on TCP-COL-HA scaffolds with 2000 kDa Mw showed greater cartilage regeneration than those seeded with 80 kDa Mw. In addition, hBMSC-seeded TCP-COL-HA scaffolds with 2000 kDa Mw showed a significantly higher mechanical strength than those with 80 kDa Mw. Collectively, these results indicate that the Mw of HA could affect chondrogenesis of BMSCs seeded on TCP-COL-HA scaffolds. The TCP-COL-HA scaffolds might be used as allogenic off the shelf products in cartilage tissue engineering in future.
- Published
- 2021
39. Autophagy inhibitors 3-MA and LY294002 repress osteoclastogenesis and titanium particle-stimulated osteolysis
- Author
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Weishen Chen, Ziji Zhang, Puyi Sheng, Baiqi Pan, Linli Zheng, Yongyu Ye, Guoyan Xian, Xiaoyu Wu, and Minghui Gu
- Subjects
Osteolysis ,Morpholines ,ATG5 ,Biomedical Engineering ,Osteoclasts ,Inflammation ,Bone resorption ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,In vivo ,Osteogenesis ,medicine ,Autophagy ,Animals ,Humans ,General Materials Science ,LY294002 ,030304 developmental biology ,Cathepsin ,Titanium ,0303 health sciences ,Chemistry ,medicine.disease ,Mice, Inbred C57BL ,Chromones ,030220 oncology & carcinogenesis ,Cancer research ,medicine.symptom - Abstract
Aseptic loosening caused by peri-implant osteolysis (PIO) is a common complication after joint replacement, and there is still no better treatment than revision surgery. The wear particle-induced inflammation response, especially subsequent osteoclastic bone resorption, is responsible for PIO. As the importance of wear particles in inducing autophagy in cells around the prosthesis in PIO has been discovered, this might be a central process underlying aseptic loosening. However, the role of autophagy induced by wear particles in osteoclastogenesis during PIO remains unclear. In this study, we investigated the role of autophagy in osteoclastogenesis and verified it in a mouse calvarial osteolysis model. We found that osteoclasts were increased in the interface membranes of patients with aseptic loosening. In vitro, knocking down the Atg5 gene or using autophagy inhibitors (3-MA, LY294002) to inhibit autophagy was found to repress osteoclastogenesis and decrease expression of the osteoclast-related genes TRAP, cathepsin K, and matrix metalloprotein 9 (MMP-9) with or without titanium (Ti) particles. In vivo, 3-MA and LY294002 repressed Ti particle-stimulated osteolysis and osteoclastogenesis and reduced expression of the pro-inflammatory factors TNF-α, IL-1β, and IL-6. Our results suggest that 3-MA and LY294002 might be the potential medicines to prevent and treat PIO and aseptic loosening.
- Published
- 2021
40. Large-scale synthesis of porous nickel boride for robust hydrogen evolution reaction electrocatalyst
- Author
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Yichen Guo, Jian Tian, Hongzhi Cui, Minghui Gu, Zhangqian Liang, Benteng Sun, Xuesong Xu, Yuxian Deng, and Yanjun Xue
- Subjects
Tafel equation ,Materials science ,General Physics and Astronomy ,Sintering ,One-Step ,02 engineering and technology ,Surfaces and Interfaces ,General Chemistry ,Electrolyte ,Overpotential ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Electrocatalyst ,01 natural sciences ,0104 chemical sciences ,Surfaces, Coatings and Films ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,0210 nano-technology ,Porosity ,Nickel boride - Abstract
Herein, porous Ni3B for efficient HER electrocatalysis can be large-scale prepared by one step sintering method using pure Ni and B powders at different temperatures in the Ar gas. Among them, Ni3B sintered at 850 °C (Ni3B-850) demonstrates the best HER activity with a low overpotential of 79 mV at current density of 10 mA cm−2 for HER in 0.5 M H2SO4 electrolyte, Tafel slope of 85.32 mV dec−1, and outstanding stability. The excellent electrocatalytic activity due to porous and similar core@shell (Ni3B@NiO/Ni(OH)2) structure, which provides larger active surface area, exposes more active sites, and facilitates electrolytes ion transfer.
- Published
- 2019
41. The pattern of drug delivery really matters
- Author
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Nan Xia, Xiaolin Huang, Jiang Xu, Minghui Gu, Ze-Jian Chen, and Yang-An Li
- Subjects
medicine.medical_specialty ,Endocrine and Autonomic Systems ,business.industry ,Immunology ,Behavioral Neuroscience ,Pharmaceutical Preparations ,Hyperalgesia ,Drug delivery ,Neuropathic pain ,medicine ,Humans ,Neuralgia ,Intensive care medicine ,business - Published
- 2021
42. Additional file 2 of Action observation treatment-based exoskeleton (AOT-EXO) for upper extremity after stroke: study protocol for a randomized controlled trial
- Author
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Zejian Chen, Xia, Nan, He, Chang, Minghui Gu, Xu, Jiang, Xiaohua Han, and Xiaolin Huang
- Subjects
Data_FILES - Abstract
Additional file 2.
- Published
- 2021
- Full Text
- View/download PDF
43. Additional file 1 of Action observation treatment-based exoskeleton (AOT-EXO) for upper extremity after stroke: study protocol for a randomized controlled trial
- Author
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Zejian Chen, Xia, Nan, He, Chang, Minghui Gu, Xu, Jiang, Xiaohua Han, and Xiaolin Huang
- Subjects
Data_FILES - Abstract
Additional file 1.
- Published
- 2021
- Full Text
- View/download PDF
44. Limited value of serum neutrophil-to-lymphocyte ratio in the diagnosis of chronic periprosthetic joint infection
- Author
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Linli Zheng, Puyi Sheng, Qiaoli Liu, Minghui Gu, Weishen Chen, Guoyan Xian, Xiaoling Chen, Baiqi Pan, Yongyu Ye, and Ziji Zhang
- Subjects
medicine.medical_specialty ,Prosthesis-Related Infections ,Neutrophils ,Arthroplasty, Replacement, Hip ,Periprosthetic ,Logistic regression ,Gastroenterology ,Sensitivity and Specificity ,Chronic periprosthetic joint infection ,Internal medicine ,Diagnosis ,medicine ,Humans ,Orthopedics and Sports Medicine ,Lymphocytes ,Neutrophil to lymphocyte ratio ,Neutrophil-to-lymphocyte ratio ,Retrospective Studies ,Orthopedic surgery ,Univariate analysis ,Receiver operating characteristic ,business.industry ,fungi ,Rheumatology ,Biomarker (medicine) ,Surgery ,Original Article ,Aseptic processing ,business ,RD701-811 ,Biomarkers - Abstract
Background Diagnosing chronic periprosthetic joint infection (PJI) is challenging. No single biomarker can accurately recognize PJI preoperatively in a timely manner. Therefore, the aim of the present study was to investigate the usefulness of the serum neutrophil-to-lymphocyte ratio (NLR) in aiding the diagnosis of chronic PJI. Materials and methods We retrospectively evaluated the medical records of 158 patients who had undergone revision arthroplasty (104 with aseptic mechanic failure and 54 with chronic PJI) from July 2011 to July 2020. Univariate analysis followed by multivariate logistic regression was applied to compare NLR, C-reactive protein (CRP), and erythrocyte sedimentation ratio (ESR) between the two groups. The receiver operating characteristic (ROC) curve was used to assess the diagnostic performance of NLR alone and in combination with CRP and ESR. Results NLR, CRP, and ESR were significantly higher in patients with chronic PJI than in the aseptic revision group (p Conclusion Compared with traditional inflammatory biomarkers (ESR and CRP), the value of serum NLR alone or combined with CRP and ESR for diagnosing chronic PJI is limited. Level of evidence Level 3.
- Published
- 2020
45. Serum Globulin and Albumin to Globulin Ratio as Potential Diagnostic Biomarkers for Periprosthetic Joint Infection: A Retrospective Review
- Author
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Weishen Chen, Baiqi Pan, Yongyu Ye, Guoyan Xian, Minghui Gu, Ziji Zhang, Linli Zheng, and Puyi Sheng
- Subjects
Male ,Reoperation ,medicine.medical_specialty ,lcsh:Diseases of the musculoskeletal system ,Prosthesis-Related Infections ,Globulin ,Periprosthetic ,Gastroenterology ,03 medical and health sciences ,0302 clinical medicine ,lcsh:Orthopedic surgery ,Internal medicine ,Diagnosis ,medicine ,Periprosthetic joint infection ,Humans ,Orthopedics and Sports Medicine ,Arthroplasty, Replacement ,Serum Albumin ,Aged ,Retrospective Studies ,030222 orthopedics ,Univariate analysis ,biology ,Receiver operating characteristic ,business.industry ,Area under the curve ,Retrospective cohort study ,Odds ratio ,Middle Aged ,Blood proteins ,Albumin to globulin ratio ,lcsh:RD701-811 ,Logistic Models ,ROC Curve ,030220 oncology & carcinogenesis ,biology.protein ,Surgery ,Female ,Serum Globulins ,lcsh:RC925-935 ,business ,Biomarkers ,Research Article - Abstract
Background Periprosthetic joint infection (PJI) has been increasingly documented; however, its preoperative accurate diagnosis remains challenging. Furthermore, there is a dire need to identify appropriate and effective biomarkers. We aimed to evaluate the relationship between globulin, albumin to globulin (A/G) ratio, and development of PJI in patients undergoing revision total joint arthroplasty (TJA). Methods A retrospective study was conducted on patients who had undergone revision TJA between 2011 and 2018 (89 with aseptic mechanic failure and 38 with PJI). The serum proteins were explored using univariate analysis followed by multivariate logistic regression. The diagnostic performance of these proteins was assessed by the receiver operating characteristic (ROC) curve. Results Higher globulin levels (odds ratio [OR], 1.239; P < 0.001) and lower A/G ratio (OR, 0.007; P < 0.001) were strongly associated with the risk of PJI. ROC curve analysis demonstrated reasonable diagnostic performance for globulin (area under the curve [AUC], 0.77; sensitivity, 78.95%; and specificity, 69.66%) and A/G ratio (AUC, 0.779; sensitivity, 65.79%; and specificity, 78.65%). Conclusions Both globulin and A/G ratio were associated with PJI and may serve as potential adjuvant biomarkers in the diagnosis of PJI.
- Published
- 2020
46. Neutrophil-Lymphocyte Ratio as A Complementary Biomarker of Periprosthetic Joint Infection: A Retrospective Review
- Author
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Yongyu Ye, Minghui Gu, Weishen Chen, Guoyan Xian, Linli Zheng, Baiqi Pan, Ziji Zhang, and Puyi Sheng
- Subjects
fungi - Abstract
Background: Diagnosing a periprosthetic joint infection (PJI) is challenging. No single biomarker can accurately recognize PJI preoperatively in a timely manner. Therefore, the aim of the present study was to investigate the usefulness of the neutrophil-lymphocyte ratio (NLR) in aiding the diagnosis of PJI.Methods: We retrospectively evaluated the medical records of 109 patients who had undergone revision arthroplasty (77 with aseptic mechanic failure and 32 with PJI) from July 2011 to November 2018. Bivariate analysis followed by multivariate logistic regression was applied to compare NLR between two groups. The receiver operating characteristic (ROC) curve was used to assess the diagnostic performance of NLR alone and in combination with C-reactive protein (CRP) and the erythrocyte sedimentation ratio (ESR).Results: NLR was significantly higher in patients with PJI than in patients of the aseptic revision group (3.12 ± 2.45 vs. 2.10 ± 1.03, p 0.05).Conclusions: NLR is associated with PJI and could be regarded as a complementary marker, which in combination with CRP could improve the diagnostic performance for PJI.
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- 2020
47. Identification of abnormally methylated-differentially expressed genes and pathways in osteoarthritis: a comprehensive bioinformatic study
- Author
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Linli Zheng, Guoyan Xian, Minghui Gu, Ziji Zhang, Yongyu Ye, Weishen Chen, Yinyue Ma, Baiqi Pan, and Puyi Sheng
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030203 arthritis & rheumatology ,business.industry ,Microarray analysis techniques ,Gene Expression Profiling ,Computational Biology ,General Medicine ,Computational biology ,Methylation ,DNA Methylation ,Collagen fibril organization ,03 medical and health sciences ,0302 clinical medicine ,Gene Ontology ,Rheumatology ,Gene expression ,DNA methylation ,Osteoarthritis ,Medicine ,Humans ,Gene Regulatory Networks ,030212 general & internal medicine ,Signal transduction ,KEGG ,business ,Gene - Abstract
To investigate abnormally methylated-differentially expressed genes (DEGs) and their related pathways in osteoarthritis (OA) by comprehensive bioinformatic analysis.Gene expression profiles of GSE51588 and GSE114007, and a gene methylation microarray data GSE63695 were downloaded from the Gene Expression Omnibus (GEO) repository. Abnormally methylated DEGs were identified. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of these genes were subsequently performed using the Database for Annotation, Visualization and Integrated Discovery (DAVID). The protein-protein interaction (PPI) network was built from STRING. Module analysis and hub gene identification were performed by using Cytoscape. Co-expression analysis was also constructed using the CEMiTool package.In total, 133 abnormally methylated DEGs were identified, including 85 hypomethylation high-expression genes and 48 hypermethylation low-expression genes. Among biological processes and KEGG pathways of abnormally methylated DEGs, collagen fibril organization was enriched most frequently, and pathways of oxidative stress and aging were enriched, including HIF-1 signaling pathway, AMPK signaling pathway, and FoxO signaling pathway. In PPI networks, the hub genes of hypomethylation high-expression genes were COL1A1, COL3A1, COL1A2, COL5A2, LUM, MMP2, SPARC, COL2A1, COL6A2, and COL7A1, and the hub genes of hypermethylation low-expression genes were VEGFA, SLC2A1, LDHA, PDK1, and BNIP3. Combined with co-expression analysis, COL3A1, LUM, and MMP2 were the critical hypomethylation high-expression hub genes in medial tibia subchondral bone.Our study implied abnormally methylated DEGs and dysregulated pathways in OA. Common methylation biomarkers included COL3A1, LUM, and MMP2, and we also found that THBS2 may serve as a novel biomarker in end-stage OA. Key Points • Abnormally methylated differentially expressed genes regulate osteoarthritis. • Hypomethylation high-expression genes were related to the extracellular matrix. • Hypermethylation low-expression genes were related to oxidative stress and aging. • COL3A1, LUM, and MMP2 were potential methylation biomarkers for osteoarthritis.
- Published
- 2020
48. Direct Growth of Antimonene on C-Plane Sapphire by Molecular Beam Epitaxy
- Author
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Shunji Xia, Minghui Gu, Ming-Hui Lu, Chen Li, Kedong Zhang, Yan-Feng Chen, Yuanfeng Ding, Yu-Sheng Wang, and Hong Lu
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Materials science ,Band gap ,sapphire ,antimonene ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,lcsh:Technology ,lcsh:Chemistry ,Adsorption ,Antimony ,molecular beam epitaxy ,Monolayer ,Molecule ,General Materials Science ,Instrumentation ,lcsh:QH301-705.5 ,Fluid Flow and Transfer Processes ,business.industry ,Plane (geometry) ,lcsh:T ,Process Chemistry and Technology ,General Engineering ,021001 nanoscience & nanotechnology ,lcsh:QC1-999 ,0104 chemical sciences ,Computer Science Applications ,chemistry ,lcsh:Biology (General) ,lcsh:QD1-999 ,lcsh:TA1-2040 ,Sapphire ,Optoelectronics ,0210 nano-technology ,business ,lcsh:Engineering (General). Civil engineering (General) ,effective adsorption ,two dimensional materials ,lcsh:Physics ,Molecular beam epitaxy - Abstract
Monolayer antimony (antimonene) has been reported for its excellent properties, such as tuneable band gap, stability in the air, and high mobility. However, growing high quality, especially large-area antimonene, remains challenging. In this study, we report the direct growth of antimonene on c-plane sapphire substrate while using molecular beam epitaxy (MBE). We explore the effect of growth temperature on antimonene formation and present a growth phase diagram of antimony. The effect of antimony sources (Sb2 or Sb4) and a competing mechanism between the two-dimensional (2D) and three-dimensional (3D) growth processes and the effects of adsorption and cracking of the source molecules are also discussed. This work offers a new method for growing antimonene and it provides ideas for promoting van der Waals epitaxy.
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- 2020
49. CD200 Mediated Anti-Inflammatory Response and Vascular Barrier Maintenance in the Development of OHSS Upon Electroacupuncture Treatment
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Li Wang, Jun Jing, Li Chen, Rujun Ma, Xie Ge, Minghui Gu, Xu Tang, Xuan Huang, and Bing Yao
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Oncology ,medicine.medical_specialty ,Electroacupuncture ,business.industry ,medicine.medical_treatment ,Reproductive medicine ,Inflammation ,Vascular permeability ,Follicular fluid ,Anti-inflammatory response ,Informed consent ,Internal medicine ,medicine ,Animal studies ,medicine.symptom ,business - Abstract
Background: Electroacupuncture (EA) reduces the inflammatory response and improves vascular permeability in hyperstimulation syndrome (OHSS) model rats, however, the mechanism still unclear. Methods: OHSS model rats were established and treated with EA, proteomic of ovaries was performed. CD200 was identified and investigated in ovaries of OHSS rat model following EA treatment. The role of CD200 in patient was evaluated in follicular fluid and serum from IVF patients. The in vitro effects of CD200 on inflammation and vascular barrier were examined in KGN and HUVEC cells. Findings: CD200 was significantly highly expressed, meanwhile, the inflammatory pathway was decreased and the vascular barrier function was improved in EA treated rats. The levels of CD200 in follicular fluid were negatively correlated with the number of oocytes retrieved and serum E2 level. In vitro, the inflammatory response in KGN cells was induced by hCG in a concentration-dependent manner, and inhibited when treated with CD200Fc (CD200R agonist). In HUVECs, the vascular barrier was improved when CM (cultural medium from KGN cell) treated with CD200Fc. Interpretations: Our findings showed the role of CD200 in the development of OHSS upon EA treatment. Meanwhile, CD200 might act as a key molecular maker in predicting the occurrence of OHSS in IVF patients. Funding Statement: This work was supported by the National Key Research and Development Program (Grant No.2018YFC1003800), the National Natural Science Foundation of China (No.81973965 and No.81904043), the Jiangsu Key Research and Development Program (Grant No.BE2018714), the Jiangsu Natural Science Foundation (Grant No.BK20191230 and No.BK20190249), China's Post-doctoral Science Foundation (Grant No.2018M643887), Special Research on Military Family Planning (Grant No.18JS004) and State Key Laboratory of Reproductive Medicine of Nanjing Medical University (Grant No.SKLRM-K201803). Declaration of Interests: The authors declare that they have no competing interests. Ethics Approval Statement: Samples were collected after obtaining the written informed consent from the patients and ethics board approval from the Nanjing Jinling Hospital (reference number 2018NZGKJ-011). All animal studies (including the rat euthanasia procedure) were done in compliance with the regulations and guidelines of Nanjing Jinling Hospital and were conducted according to the AAALAC and the IACUC guidelines (reference number 20140729).
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- 2020
50. SPHK-2 Promotes the Particle-Induced Inflammation of RAW264.7 by Maintaining Consistent Expression of TNF-α and IL-6
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Ziqing Li, Guoyan Xian, Weiming Lai, Wenhua Liu, Guangpu Yang, Weishen Chen, Haixing Wang, Minghui Gu, Yinbo Xiao, Puyi Sheng, and Ziji Zhang
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0301 basic medicine ,Necrosis ,Immunology ,Sphingosine kinase ,Inflammation ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,medicine ,Animals ,Immunology and Allergy ,Interleukin 6 ,Titanium ,biology ,Sphingosine ,Interleukin-6 ,Tumor Necrosis Factor-alpha ,Chemistry ,Sphingosine Kinase 2 ,Interleukin ,Prosthesis Failure ,Phosphotransferases (Alcohol Group Acceptor) ,RAW 264.7 Cells ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer research ,biology.protein ,Particulate Matter ,Tumor necrosis factor alpha ,medicine.symptom - Abstract
Aseptic implant loosening is a devastating long-term complication of total joint arthroplasty. It is mainly initiated by the interaction of wear debris and macrophages. However, how does the chronic inflammation persist and how to stop it is poorly understood. Sphingosine kinases (SPHKs) are an essential feature of immunosuppressive M2 polarisation in macrophages and a promoter for chronic inflammation. In this study, RAW 264.7 macrophages were exposed to stimulation with titanium particles (0.1 mg/ml), and the subsequent expression of SPHKs and pro-inflammatory cytokines was evaluated. The effect of inhibitors of SPHKs (FTY720, PF543, and ABC294640) on titanium particle-challenged macrophages was analysed. As for results, the amount of sphingosine kinase (SPHK)-1 and SPHK-2 in RAW264.7 macrophages increased in the presence of titanium particles in a time-dependent manner. Two inhibitors of SPHKs (FTY720 and ABC294640) suppressed titanium particle-induced tumour necrosis factor (TNF)-α and interleukin (IL)-6 production in RAW264.7 macrophages. These findings suggest that persistent stimulation with titanium particles may lead to a consistent release of TNF-α and IL-6 via SPHK-2 activity, which may lead to aseptic implant loosening. Appropriate regulation of SPHK-2 may serve as a potential new strategy in the treatment of aseptic implant loosening.
- Published
- 2018
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