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A novel DLC film deposition method was proposed to realize the deposition of DLC film on the surface of complex shaped workpiece. Meanwhile, Si-DLC film was deposited on the surface of M2 high-speed steel (HSS M2) and 304 stainless steel (304SS), and the microstructure, surface roughness, mechanical properties, corrosion resistance and tribological properties of Si-DLC films were characterized in detail. Results show that Si-DLC film at different axial positions of the auxiliary cathode possesses similar microstructure, film thickness and surface roughness, and the as-deposited Si-DLC film shows the low intrinsic stress of

BACKGROUND: Studies of neurologic outcomes have found conflicting results regarding differences between patients with substance-related cardiac arrests (SRCA) and non-SRCA. We investigate the effects of SRCA on severe cerebral edema development, a neuroimaging intermediate endpoint for neurologic injury. METHODS: 327 out-of-hospital comatose cardiac arrest patients were retrospectively analyzed. Demographics and baseline clinical characteristics were examined. SRCA categorization was based on admission toxicology screens. Severe cerebral edema classification was based on radiology reports. Poor clinical outcomes were defined as discharge Cerebral Performance Category scores>3. RESULTS: SRCA patients (N=86) were younger (P

Liver fibrosis is the common consequence of almost all liver diseases and has become an urgent clinical problem without efficient therapies. Recent evidence has shown that hepatocytes-derived extracellular vesicles (EVs) play important roles in liver pathophysiology, but little is known about the role of damaged hepatocytes-derived EVs in hepatic stellate cell (HSC) activation and following fibrosis. Tetramethylpyrazine (TMP) from Ligusticum wallichii Franchat exhibits a broad spectrum of biological activities including liver protection. In this study, we investigated whether TMP exerted liver-protective action through regulating EV-dependent intercellular communication between hepatocytes and HSCs. Chronic liver injury was induced in mice by CCl

The unique cultural phenomenon of Asia and the growth of Asian enterprises have attracted considerable academic attention in this domain. Clan culture is a typical part of Asian culture and represents a new field in the cultural study of management. Based on the statistical data from China's Annual Census on Industrial Enterprises 2007, this article discusses the influence of clan culture on the financial and social performance of private-owned enterprises (POEs). The results of this study show that the strength of clan culture inhibits the financial performance of POEs and improves social performance—i.e., the welfare of their internal employees; in addition, the degree of regional marketization and social trust can moderate this relationship. In summary, we illuminate the institutional logic that clan protection affects POE performance.

PurposeBased on the transaction cost theory, this paper aims to investigate the impact of cultural distance on international strategic alliance formation and its underlying mechanisms.Design/methodology/approachThis paper uses the investment of foreign firms in the Chinese Venture Capital market as an empirical background, Obtaining VC data from Zero2IPO Private Equity, CVsource Investment Database (2001–2015). This paper chooses the Logit regression method, according to Lind’s three-step method to test the inverted U-shaped relationship.FindingsThe empirical analysis of foreign venture capital firms invested in China revealed that there is an inverted U-shaped relationship between cultural distance and the possibility of international strategic alliances. This relationship is the result of two opposing mechanisms, which are the need and the feasibility of international strategic alliances. In addition, this study further examined the moderating effects of social embeddedness and social reputation, revealing the boundary effects on the complex relationship between cultural distance and possible international strategic alliance formation.Originality/valueThis study focuses on cultural difference, which is a key factor leading to a firm’s transaction costs. Based on the transaction cost theory, this paper investigates the impact of cultural distance on international strategic alliance formation and its underlying mechanisms.

A reliable, high-performance coating procedure was developed using PDMS to modify a duplex MAO/DLC coating on an AZ31B Mg alloy. First, the duplex MAO/DLC coating was fabricated via a combined MAO and unbalanced magnetron sputter process. Subsequently, a PDMS solution was used to modify the MAO/DLC coating via a conventional dip-coating method. The surface characteristics, bond strength, hardness, tribological behaviour, and corrosion resistance of the coated samples were evaluated via SEM, CA, Raman spectroscopy, friction and wear behaviour, polarisation curve, and NSS tests. The PDMS modification reduced the HIT of MAO/DLC coating from 15.96 to 8.34 GPa; this is ascribed to the penetration of PDMS, which has good rheological properties to form a viscoelastic Si-based organic polymer layer on the MAO/DLC coating. However, the PDMS-modified MAO/DLC coating was denser, hydrophobic, and had higher bond strength compared with MAO- and MAO/DLC-coated samples. Moreover, the PDMS modification reduced the COF and wear rate of the duplex MAO/DLC coating. This indicates that the PDMS improved the tribological behaviour owing to the transferred Si oxide that originated from the Si-O network of the PDMS, as well as the low graphitisation of the DLC layer during sliding. Furthermore, the corrosion current density of the MAO/DLC-coated sample modified by PDMS for 10 min decreased by two order of magnitude compared with that of the MAO/DLC-coated sample but by five orders of magnitude compared with that of the bare substrate. The NSS tests proved that the PDMS layer slowed the corrosion of the Mg alloy under long-term service, enhancing the corrosion protection efficiency. The results are attributed to the high bond strength and lubricant MAO/DLC layer, and the dual role of sealing and hydrophobicity of PDMS. Therefore, PDMS modification is promising for the fabrication of protective materials for Mg alloys that require corrosion and wear resistance.

High-quality tea requires leaves of similar size and tenderness. The grade of the fresh leaves determines the quality of the tea. The automated classification of fresh tea leaves improves resource utilization and reduces manual picking costs. The present study proposes a method based on an improved genetic algorithm for identifying fresh tea leaves in high-speed parabolic motion using the phenotypic characteristics of the leaves. During parabolic flight, light is transmitted through the tea leaves, and six types of fresh tea leaves can be quickly identified by a camera.The influence of combinations of morphology, color, and custom corner-point morphological features on the classification results were investigated, and the necessary dimensionality of the model was tested. After feature selection and combination, the classification performance of the Naive Bayes, k-nearest neighbor, and support vector machine algorithms were compared. The recognition time of Naive Bayes was the shortest, whereas the accuracy of support vector machine had the best classification accuracy at approximately 97%. The support vector machine algorithm with only three feature dimensions (equivalent diameter, circularity, and skeleton endpoints) can meet production requirements with an accuracy rate reaching 92.5%. The proposed algorithm was tested by using the Swedish leaf and Flavia data sets, on which it achieved accuracies of 99.57% and 99.44%, respectively, demonstrating the flexibility and efficiency of the recognition scheme detailed in the present study.This research provides an efficient tea leaves recognition system that can be applied to production lines to reduce manual picking costs. © 2022 Society of Chemical Industry.

The proteome and its time-dependent effects reveal the importance of stress response (including expression regulation of heat-shock proteins) and fatty acid metabolism in cold adaptation and preservation of Hami melon. To better understand the molecular mechanism of how Hami melons respond to low-temperature stress, this study investigated the relevant physiological characteristics, catalytic antibody activity, and quantitative proteomics of Hami melon (Jiashi muskmelon) during low-temperature storage. Jiashi muskmelon was stored inside two refrigerators set at 21 °C (control group) and 3 °C, respectively, for 24 days. Low-temperature storage led to a significantly reduced decay rate, weight loss rate, and loss of relative conductivity. It also maintained fruit firmness, inhibited the production rate of malondialdehyde and H

Hami melon (Cucumis melo L. var. inodorus Jacq) is an important horticultural crop but susceptible to chilling injury during low temperature storage. To characterize the gene expression profiles of Gold Queen Hami melon under the cold stress and discover the key cold stress-induced genes, five cDNA libraries, C0 (control), C12 (control for 12 d), C24 (control for 24 d), L12 (cold treatment at 0.5°C for 12 d) and L24 (cold treatment at 0.5°C for 24 d), were constructed for RNA sequencing (RNA-Seq) and gene expression profiling. A total of 6479 and 7914 differentially expressed genes (DEGs) in L12 and L24 compared with C0 were respectively detected. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses identified the relevant biological functions and metabolic pathways of these differentially expressed genes. In addition, a diverse range of cold response candidate genes were mainly obtained from starch and sucrose metabolism, pentose phosphate pathway and glycolysis/gluconeogenesis, such as genes encoding sucrose-phosphate synthase, α-amylase, β-amylase, sucrose synthase and glucose-6-phosphate dehydrogenase. Various transcription factors with divergent expression patterns were identified. Finally, quantitative real time polymerase chain reaction (qRT-PCR) was conducted to validate the RNA-Seq and gene differentially expression results. This study provides valuable information for further studies on cold response mechanisms and genetic improvement of cold tolerance in Gold Queen Hami melons.

In this paper, an improved estimation of signal parameters via rotational invariance techniques (ESPRIT) algorithm is proposed. As to the frequency scanning leaky wave antenna (LWA), the direction of arrival (DOA) can be effectively estimated by the algorithm. Compared with ESPRIT algorithm based on array antennas or electronically controlled beam scanning LWA, the ESPRIT algorithm using frequency scanning LWA obviously simplifies the hardware implementation. Furthermore, the wideband DOA can be simultaneously estimated by this proposed algorithm. The simulation results show that the estimated DOA results are in good agreement with the predicted angles, with root mean square error (RMSE) less than 1.6% on 38% relative bandwidth (ultra wide band).

Background Cantaloupe is susceptible to cold stress when it is stored at low temperatures, resulting in the loss of edible and commercial quality. To ascertain the molecular mechanisms of low temperatures resistance in cantaloupe, a cold-sensitive cultivar, Golden Empress-308 (GE) and a cold-tolerant cultivar, Jia Shi-310 (JS), were selected in parallel for iTRAQ quantitative proteomic analysis. Results The two kinds of commercial cultivars were exposed to a temperature of 0.5 °C for 0, 12 and 24 days. We found that the cold-sensitive cultivar (GE) suffered more severe damage as the length of the cold treatment increased. Proteomic analysis of both cultivars indicated that the number of differentially expressed proteins (DEPs) changed remarkably during the chilly treatment. JS expressed cold-responsive proteins more rapidly and mobilized more groups of proteins than GE. Furthermore, metabolic analysis revealed that more amino acids were up-regulated in JS during the early phases of low temperatures stress. The DEPs we found were mainly related to carbohydrate and energy metabolism, structural proteins, reactive oxygen species scavenging, amino acids metabolism and signal transduction. The consequences of phenotype assays, metabolic analysis and q-PCR validation confirm the findings of the iTRAQ analysis. Conclusion We found that the prompt response and mobilization of proteins in JS allowed it to maintain a higher level of cold tolerance than GE, and that the slower cold responses in GE may be a vital reason for the severe chilling injury commonly found in this cultivar. The candidate proteins we identified will form the basis of future studies and may improve our understanding of the mechanisms of cold tolerance in cantaloupe.

Flavoalkaloids are a unique class of compounds in tea, most of which have an

BACKGROUND Bilioenteric Roux-en-Y anastomosis is one of the most complicated approaches for reconstructing the gastrointestinal tract, and endoscopic retrograde cholangiopancreatography (ERCP) is technically challenging in patients after bilioenteric Roux-en-Y anastomosis. The optimal endoscopic strategies for such cases remain unknown. AIM To explore the feasibility and effectiveness of single balloon enteroscopy-assisted (SBE-assisted) therapeutic ERCP in patients after bilioenteric Roux-en-Y anastomosis based on multi-disciplinary collaboration between endoscopists and surgeons as well as report the experience from China. METHODS This is a single center retrospective study. All of the SBE-assisted therapeutic ERCP procedures were performed by the collaboration between endoscopists and surgeons. The operation time, success rate, and complication rate were calculated. RESULTS Forty-six patients received a total of 64 SBE-assisted therapeutic ERCP procedures, with successful scope intubation in 60 (93.8%) cases and successful diagnosis in 59 (92.2%). All successfully diagnosed cases received successful therapy. None of the cases had perforation or bleeding during or after operation, and no post-ERCP pancreatitis occurred. CONCLUSION Based on multi-disciplinary collaboration, SBE-assisted therapeutic ERCP in patients after bilioenteric Roux-en-Y anastomosis is relatively safe and effective and has a high success rate.

Calcium ions are second messengers in plant signal transduction involved in plant growth and development and the response to stress. Calcium-dependent protein kinase (CDPK) is calcium ion receptor functioning in plant disease resistance. Herein, we investigated changes in CDPK activity and resistance to Penicillium in postharvest Hami melons after different treatments. The non-infection group was permeated with calcium chloride (CaCl2) solution, while the infection group was permeated with CaCl2 solution and infected with Penicillium. CDPK activity was elevated in both groups but was higher in the infection group. Analysis of the molecular mechanism of CPDK in Penicillium infection revealed the 1635-bp HmCDPK2 gene with an open reading frame of 1239 nucleotides, encoding a protein of 412 amino acids with a molecular weight of 46161.61 Da and a theoretical isoelectric point of 5.00. The HmCDPK2 protein shares 40.11% tertiary structure similarity with CDPK from the apicomplexan parasite Plasmodium Bergheii (PDB ID 3Q5I ). Fluorescence quantitative PCR results showed that expression of HmCDPK2 was upregulated in the infection group relative to the non-infection group, peaking at 12 h after treatment. We conclude that Hami melon develops resistance to Penicillium infection by regulating CDPK activity and upregulating HmCDPK2 expression.

This study evaluated the protection against corrosion and wear afforded to AZ91D Mg alloy by coatings of hydrogen-free and low-Cr-doped hydrogenated diamond-like carbon (DLC) films. The microstructure and corrosion resistance were evaluated using scanning electron microscopy, atomic force microscopy, Raman spectroscopy, polarization curves, and neutral salt spraying tests. Wear tests were performed to investigate the friction and wear behaviors of the samples against 9Cr18 in humid air, deionized water, and 3.5 wt.% NaCl solution using a reciprocating sliding test in the ball-on-disk mode. The results showed that the more compact Cr-H-DLC film improved the corrosion resistance of the Mg alloy, whereas the Cr-DLC film accelerated the corrosion in the 3.5 wt.% NaCl solution. All Mg alloy samples coated with DLC films exhibited low coefficient of friction (COF) values and smaller wear volumes compared with those of bare substrate in air, water, or NaCl solution. The Cr-DLC film presented the lowest COF and wear rate in air, but the worst corrosion protection in air and NaCl solution, whereas the converse was found for the Cr-H-DLC film. Unfortunately, all coated samples showed limited protection ability because of pore defects in the films, high galvanic potential between the substrate and the buffer layer or film, and high electrical conductivity, which caused severe tribocorrosion of the Mg alloy during the wear tests in NaCl solution. The corrosion protection ability of DLC films is key to the wear resistance protection of Mg alloys in water or NaCl solution.

Low temperature storage prolongs the postharvest life of various fresh fruits and vegetables. However, the actual storage temperature may also significantly influence fruit and vegetable quality. In the present study, we stored Gold Queen Hami melons at three different temperatures [21 °C (control), 3 °C, and 0.5 °C (0 ± 0.5 °C)] for 36 days. The humidity for all three temperatures conditions was between 75% and 85%. The results indicated a correlation between chilling injury in Gold Queen Hami melons and storage temperature and time. Different degrees of chilling injury in Gold Queen Hami melons were observed after storage at 3 °C and 0.5 °C, whereas these were detected later when stored at 3 °C. In addition, the rotting rate, weight loss rate, and chilling injury index at 3 °C were significantly lower compared to that at 0.5 °C storage temperature. Compared to other temperatures, storage at 3 °C was associated with a delay in fruit rotting, sustained high fatty acid desaturase and adenosine triphosphatase (ATPase) activity, a decrease in lipoxygenase activity, enhanced cold resistance, and extended total storage period. At 21 °C, no chilling injury was observed, although extensive weight loss and high rotting rates were detected. Moreover, two highly expressed genes, namely, JhMYB24 and JhMYB48, were screened and selected from the Gold Queen Hami melon transcriptome database. Real-time PCR analysis showed that the expression of JhMYB24 and JhMYB48 continuously increased under cold stress and peaked at the 18th day of cold storage. The expression of JhMYB24 and JhMYB48 at 3 °C was significantly higher than that at 0.5 °C or 21 °C. Together, our results demonstrate that regulating enzyme activity and upregulating the expression of JhMYB24 and JhMYB48 alleviate cold stress in fruits stored at 3 °C, thereby maintaining high fruit quality.

Non-small cell lung cancer (NSCLC) is the most common cancer in the world. Gefitinib, an inhibitor of EGFR tyrosine kinase, is highly effective in treating NSCLC patients with activating EGFR mutations (L858R or Ex19del). However, despite excellent disease control with gefitinib therapy, innate resistance and inevitable acquired resistance represent immense challenges in NSCLC therapy. Gefitinib potently induces cytoprotective autophagy, which has been implied to contribute to both innate and acquired resistance to gefitinib in NSCLC cells. Currently, abrogation of autophagy is considered a promising strategy for NSCLC therapy. In the present study, YC-1, an inhibitor of HIF-1α, was first found to significantly inhibit the autophagy induced by gefitinib by disrupting the fusion of autophagosomes and lysosomes and thereby enhancing the proapoptotic effect of gefitinib in gefitinib-resistant NSCLC cells. Furthermore, the combinational anti-autophagic and pro-apoptotic effect of gefitinib and YC-1 was demonstrated to be associated with an enhanced of forkhead box protein O1 (FOXO1) transcriptional activity which resulted from an increase in the p-FOXO1 protein level in gefitinib-resistant NSCLC cells. Our data suggest that inhibition of autophagy by targeting FOXO1 may be a feasible therapeutic strategy to overcome both innate and acquired resistance to EGFR-TKIs.

Introduction: The incidence of substance-related cardiac arrests (SRCA) has increased due to the opioid epidemic. Previous studies have shown that SRCA present differently than non-SRCA. Weinvestigated differences in the clinical courses between the two groups for patients who were initially comatose post-resuscitation. Methods: Data from out-of-hospital arrest patients seen between 2007-2019 were retrospectively analyzed. Arrests were classified as SRCA if the patient had a positive toxicology screen, witnesses testified to substance use, or drug paraphernalia was present at the scene. Arrest types were categorized as shockable or non-shockable. Cerebral edema classifications were based on MRI or CT radiology reports. Poor outcomes were defined as Cerebral Performance Category (CPC) > 3 at discharge. Differences in demographics, presentation and outcomes were compared (Fisher’s Exact Test or Exact Wilcoxon-Mann-Whitney). Multivariable backward stepwise logistic regression was performed. Results: SRCA patients (N=90) were younger (p Discussion: SRCA patients are more likely to have non-shockable arrests which in turn leads to cerebral edema development and potentially worse outcomes.

Conventional analysis, electronic senses and HS-SPME-GC-MS were applied to evaluate the effects of pretreatment methods and leaching methods on jujube wine quality. Significant differences (p 0.05) in the levels of alcohol content, color, taste and aroma were observed among all the jujube wine samples, in which the pulp and pectase fermented jujube wine was the best among all. Moreover, rather than taste, aroma is the most significantly (p 0.05) affected. In regard to aroma, a total of 182 volatile compounds were identified by HS-SPME-GC-MS. It was found that the blended-into-pulp treatment and the leached-by-pectase treatment had notable positive effects on jujube wine. The pulp and pectase fermented jujube wines exhibited the highest concentration of total volatile compounds as well as alcohols, esters, acids and aldehydes. Thus, the optimal pretreatment method and leaching method for jujube wine fermentation are blended-into-pulp and leached-by-pectase respectively.

Modified citrus pectin (MCP) is a carbohydrate enriched complex, which has been implicated in cancer treatment and prevention. However, the effects of MCP on urinary bladder cancer (UBC) are unknown. In this study, MCP was first tested in T24 and J82 human UBC cells and showed the inhibition of cell viability by the sulforhodamine B (SRB) assay. The MCP-treated UBC cells exhibited G(2)/M phase arrest with the decrease of Cyclin B1 and phosphorylated Cdc2. Caspase-3 was also activated, leading to the cleavage of Caspase-3 and PARP. We further explored the possible molecular mechanisms upon MCP treatment in UBC cells. Reduction of galectin-3 was observed and followed with the inactivation of Akt signaling pathway. Of note, galectin-3 knockdown by RNA interference recapitulated the MCP-mediated anti-proliferation, cell cycle arrest and apoptosis. Moreover, oral administration of MCP to the T24 xenograft-bearing nude mice inhibited the tumor growth significantly (P