1. Effects of angle of incidence of stimulus light on photopic electroretinograms of zebrafish larvae
- Author
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Hisashi Matsubara, Shinichiro Chujo, Yoko Mase, Yukiko Muramoto, Kumiko Kato, and Mineo Kondo
- Subjects
Zebrafish ,Electroretinography ,ERG ,Light stimulation ,Fiber optical cable ,Full-field stimulation ,Medicine ,Science - Abstract
Abstract In electroretinographic (ERG) recordings of zebrafish, the light stimulus is usually delivered by a fiber optic cable. The purpose of this study was to determine whether the angle of incidence of the stimulus light from the fiber optic cable will affect the amplitudes and implicit times of the ERGs of zebrafish larvae. The larvae were positioned on their side with the right eye pointed upward. The light stimuli were delivered by a fiber optic cable from three directions of the larvae: frontal 0° (F0°), dorsal 30°(D30°), and ventral 30°(V30°). Photopic ERGs were recorded from 16 larvae at age 5–6 days post-fertilization. Our results showed that the mean amplitude of the b-wave elicited at D30° and V30° stimulation was significantly smaller than that elicited at F0° stimulation (P = 0.014 and P = 0.019, respectively). In addition, the mean amplitude of the d-wave elicited at D30° and V30° stimulation was significantly smaller than that elicited at F0° stimulation (P
- Published
- 2024
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