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11. Long-term stability of 24 synthetic cannabinoid metabolites spiked into whole blood and urine for up to 168 days, and the comparable study for the 6 metabolites in non-spiked real case specimens stored for several years

Author

Minakata, Kayoko, Hasegawa, Koutaro, Nozawa, Hideki, Yamagishi, Itaru, Miyoshi, Naotomo, Suzuki, Masako, Kitamoto, Takuya, Kondo, Minako, Watanabe, Kanako, and Suzuki, Osamu

Subjects
Human authentic specimens, LC–MS/MS, Blood and urine, Long-term stability, Deep-freezing, Synthetic cannabinoid metabolite
Abstract

Purpose The aim of this study is to investigate the stabilities of the 24 synthetic cannabinoid metabolites (SCMs) in blood and urine at various temperatures from –30 to 37℃ stored for 1 to 168 days. In addition, experiments of stabilities at lower temperatures and for much longer duration have been performed as described below.Methods The quantification was performed by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The blank blood and urine spiked with SCMs and non-spiked real case (authentic) specimens were incubated at 37℃ up to 56 days and at 22, 4 or –30℃ up to 168 days. The non-spiked authentic blood and urine specimens were also stored at –30 or –80℃ for 1, 3 or 5 years to investigate stabilities during very long time frames.Results All the 24 SCMs were much more stable in urine than in blood at 37, 22 or 4℃. All 24 SCMs spiked into blood or urine were stable at –30℃ for up to 168 days. The 6 SCMs in the authentic specimens exhibited long stabilities at –30 or –80℃ for 3 to 5 years. Some tendencies were observed according to the relation between the structures of SCMs and their stabilities.Conclusions The long-term stabilities of 24 SCMs in spiked samples and those of 6 SCMs in the authentic specimens were examined using LC–MS/MS. SCMs were largely very stable and usable several years after storage at –30 or –80℃.

Published
2022

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