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This study investigates caffeine degradation and mineralisation using Fenton and photo-Fenton processes through a 2k Factorial Design. Key parameters varied include Fe2+ concentration (2 and 10 mg/L), H₂O₂ concentration (50 and 500 mg/L), light intensity (0 and 96 W), and reaction time (0 and 60 min) to optimise degradation. The experimental setup featured a 1 L glass contact column in a UV-controlled chamber with an initial caffeine concentration of 20 mg/L. Results revealed that Fenton was inefficient, achieving only 12% degradation and 9% mineralisation, with dimethylparabanic acid as the main by-product. Conversely, photo-Fenton attained 88% degradation and 40% mineralisation, demonstrating the significant influence of UV light. Energy consumption varied from 3.33 to 50 kWh.m−3. Optimal conditions identified were 10 mg/L Fe2+, 50 mg/L H₂O₂, 96 W UV intensity, and a 60-minute reaction time, highlighting the effectiveness of advanced photo-Fenton oxidation for caffeine treatment. [ABSTRACT FROM AUTHOR]

This study evaluates the quality of drinking groundwater in the city of Moussoro using geochemical, bacterial, and multivariate statistical methods. Access to safe drinking water is a major concern for the population in these regions, as the distribution of this resource is unequal across continents and is often affected by human activities, making it vulnerable. The analysis revealed two categories of water: potable and non-potable. The presence of pollutant indicators, such as Escherichia coli (E. coli) and total coliforms, was observed in most samples. This presence indicates water contamination and requiring monitoring and treatment before it is consumed by the population. Overall, most of the physicochemical parameters comply with the World Health Organisation guidelines for drinking water. However, the concentration of ammonium (NH4+) was high. Pollution elements such as ammonium (NH4+) and E. coli, indicate that human activities also contribute to water mineralisation. The study could ensure sustainable access to safe drinking water, thereby enhancing the overall quality of life in Moussoro. [ABSTRACT FROM AUTHOR]

Background: Dental caries remains a significant global health problem. One of the fundamental mechanisms underlying the development and progression of dental caries is the dynamic process of demineralisation/remineralisation. In vitro models have played a critical role in advancing our understanding of this process and identifying potential interventions to prevent or arrest dental caries. This literature review aims to provide a structured oversight of in vitro mineralisation models which have been used to study the tooth demineralisation/remineralisation process. Methods: Publications from 2019 to 2023 were screened to identify articles reporting the use of in vitro models to study the demineralisation/remineralisation of tooth caries. The included studies were methodologically assessed for their information on (i) substrate, (ii) lesion formation, and (iii) mineralisation models. Results: The most reported substrates used in the studies were human teeth along with bovine incisors. Acetic/lactic buffers were the most common solutions to induce caries lesions. pH cycling was the most frequently used mineralisation model for simulating the daily change within the oral environment. This review discussed the advantages and limitations of various approaches. Conclusions: Standardisation of in vitro mineralisation models is crucial for enabling effective comparison between studies and advancing caries research. [ABSTRACT FROM AUTHOR]

Porous titanium scaffolds fabricated by powder bed fusion additive manufacturing techniques have been widely adopted for orthopedic and bone tissue engineering applications. Despite the many advantages of this approach, topological defects inherited from the fabrication process are well understood to negatively affect mechanical properties and pose a high risk if dislodged after implantation. Consequently, there is a need for further post‐process surface cleaning. Traditional techniques such as grinding or polishing are not suited to lattice structures, due to lack of a line of sight to internal features. Chemical etching is a promising alternative; however, it remains unclear if changes to surface properties associated with such protocols will influence how cells respond to the material surface. In this study, we explored the response of bone marrow derived mesenchymal stem/stromal cells (MSCs) to Ti‐6Al‐4V whose surface was exposed to different durations of chemical etching. Cell morphology was influenced by local topological features inherited from the SLM fabrication process. On the as‐built surface, topological nonhomogeneities such as partially adhered powder drove a stretched anisotropic cellular morphology, with large areas of the cell suspended across the nonhomogeneous powder interface. As the etching process was continued, surface defects were gradually removed, and cell morphology appeared more isotropic and was suggestive of MSC differentiation along an osteoblastic‐lineage. This was accompanied by more extensive mineralization, indicative of progression along an osteogenic pathway. These findings point to the benefit of post‐process chemical etching of additively manufactured Ti‐6Al‐4V biomaterials targeting orthopedic applications. [ABSTRACT FROM AUTHOR]

Abstract This study evaluates the quality of drinking groundwater in the city of Moussoro using geochemical, bacterial, and multivariate statistical methods. Access to safe drinking water is a major concern for the population in these regions, as the distribution of this resource is unequal across continents and is often affected by human activities, making it vulnerable. The analysis revealed two categories of water: potable and non-potable. The presence of pollutant indicators, such as Escherichia coli (E. coli) and total coliforms, was observed in most samples. This presence indicates water contamination and requiring monitoring and treatment before it is consumed by the population. Overall, most of the physicochemical parameters comply with the World Health Organisation guidelines for drinking water. However, the concentration of ammonium (NH4+) was high. Pollution elements such as ammonium (NH4+) and E. coli, indicate that human activities also contribute to water mineralisation. The study could ensure sustainable access to safe drinking water, thereby enhancing the overall quality of life in Moussoro.

Iris Xiaoxue Yin,1 John Yun Niu,1 May Lei Mei,2 Jinyao Tang,3 William Ka Kei Wu,4 Chun Hung Chu1 1Faculty of Dentistry, the University of Hong Kong, Hong Kong, People’s Republic of China; 2Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, Dunedin, New Zealand; 3Department of Chemistry, the University of Hong Kong, Hong Kong, People’s Republic of China; 4Department of Anaesthesia & Intensive Care, The Chinese University of Hong Kong, Hong Kong, People’s Republic of ChinaCorrespondence: May Lei Mei, Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, Department of Oral Rehabilitation, Faculty of Dentistry, University of Otago, PO Box 56, Dunedin, Otago, 9054, New Zealand, Email may.mei@otago.ac.nz Chun Hung Chu, Faculty of Dentistry, The University of Hong Kong, Restorative Dental Science, Faculty of Dentistry, The University of Hong Kong, 34 hospital Road, Hong Kong, NA, People’s Republic of China, Email chchu@hku.hkObjective: The objectives of this study were to develop a tooth-binding graphene quantum dots silver nanocomposites (ALN-GQDs-Ag) and evaluate their antibacterial, mineralising, and discolouring properties for the prevention of dental caries.Methods: In this study, ALN-GQDs-Ag were developed by synthesising nano silver (Ag) with graphene quantum dots (GQDs) and functionalised GQDs with alendronate (ALN). ALN-GQDs-Ag were characterised by transmission electron microscopy (TEM), zeta potential analysis, X-ray photoelectron spectroscopy (XPS), Fourier transform infrared spectroscopy (FTIR), and Raman spectroscopy. The cytotoxicity of ALN-GQDs-Ag against human gingival fibroblasts (HGF-1) and stem cells from human exfoliated deciduous teeth (SHED) was examined using a colorimetric assay with reference to silver nitrate solution. The affinity of ALN-GQDs-Ag for hydroxyapatite particles was investigated using inductively coupled plasma spectroscopy (ICP). The antibacterial properties of ALN-GQDs-Ag against Streptococcus mutans were evaluated by scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), and colony-forming units counting (CFUs). The mineralisation properties of ALN-GQDs-Ag on human dentine were assessed using micro-computed tomography (micro-CT), scanning electron microscopy (SEM), and Fourier transform infrared in a biochemical cycling model. The discolouring properties of ALN-GQDs-Ag on artificial dentine caries were determined using spectrophotometry.Results: TEM, Zeta potential, XPS, FTIR, and Raman spectroscopy confirmed the synthesis of stable spherical ALN-GQD-Ag nanocomposites with a 10.3 ± 5.5 nm diameter. The colorimetric assay demonstrated that ALN-GQDs-Ag were less cytotoxic than silver nitrate to HGF-1 and SHED (p< 0.001). ICP showed that ALN-GQDs-Ag were bound to hydroxyapatite. SEM, CLSM, and CFUs showed that ALN-GQDs-Ag was bactericidal and inhibited biofilm growth of Streptococcus mutans. Micro-CT, SEM, and FTIR showed that ALN-GQDs-Ag repressed dentine demineralisation under a cariogenic challenge. Spectrophotometry revealed no significant discolouration of dentine caries in the ALN-GQDs-Ag.Conclusion: This study developed a biocompatible and tooth-binding ALN-GQDs-Ag with promising antibacterial, mineralising, and non-discolouring properties. ALN-GQDs-Ag could be a novel anti-caries agent for preventing dentine caries if translated for clinical use.Keywords: graphene quantum dots, silver nanoparticles, caries prevention, antibacterial, mineralisation

Abstract The demand for sustainable development goals and the absence of systematic development and organised exploration for gold has prompted this study to integrate magnetic and radiometric datasets with lithology to evaluate the gold mineralisation potential in the Ilesha schist belt. This study considers 3168.72 km2 of the Ilesha schist belt in southwestern Nigeria, a frontier belt for gold deposits. The high-resolution airborne magnetic and radiometric datasets were processed using enhancement techniques, including the analytical signal, lineament density, and K/Th ratio. CET grid analysis, Euler deconvolution, and analytical signal depth estimation methods were used to aid the interpretation. The spatial integration and interpolation were performed using the Analytical Hierarchy Process (AHP) and weighted overlay analytical tools within the ArcGIS environment. The dominant structural controls for potential mineralisation are ENE–WSW and ESE–WNW trends. The depth of the magnetic sources revealed by the analytical signal ranged from 63.17 to 629.47 m, while depths ranging from 47.32 to 457.22 m were obtained from Euler deconvolution. The delineated highly magnetic edge sources, dense lineaments, radiometrically highlighted alteration zones, and lithological hosts for gold mineralisation were integrated to establish the gold mineralisation potential map. The AHP deductions reveal that 10.52% of the study site is within the high mineralisation potential class, a remarkable 60.39% falls within the moderate class, a significant portion (28.86%) falls within the poor class, and 0.23% is considered unfavourable. The result was optimised by validation using known mines, with 94% (i.e., 15 out of 16 mining sites) plotting within the high mineralisation potential class. This assessment provides invaluable insight for stakeholders and policymakers to embark on gold exploration and exploitation and promote sustainable mineral development.

Per- and polyfluoroalkyl substances (PFAS) are a group of fluorinated synthetic chemicals that are highly recalcitrant, toxic, and bio-accumulative and have been detected in biosolids worldwide, posing potential risks to humans and the environment. Recent studies suggest that the organic C-F bond in PFAS can be destructed and potentially mineralised into inorganic fluorides during thermal treatment. This study focuses on thermodynamic equilibrium investigations and the fate of fluorine compounds post-PFAS destruction during biosolid thermal treatment. The results indicate that gas-phase fluorine compounds are mainly hydrogen fluoride (HF) and alkali fluorides, whereas solid-phase fluorine compounds include alkaline earth fluorides and their spinels. High moisture and oxygen content in the volatiles increased the concentration of HF in the gas phase. However, adding minerals reduced the emission of HF in the gas phase significantly and enhanced the capture of fluorine as CaF2 spinel in the solid phase. This study also investigates the effect of feedstock composition on the fate of fluorine. High ash content and low volatile matter in the feedstock reduced HF gas emissions and increased fluorine capture in the solid product. The findings of this work are useful in designing thermal systems with optimised operating conditions for minimising the release of fluorinated species during the thermal treatment of PFAS-containing biosolids. [ABSTRACT FROM AUTHOR]

Water resources are facing significant challenges in result of rapidly growing demand, deteriorating quality, and the effects of climate change. Today, water quantity and quality issues have become prevalent in various regions across the globe, affecting both northern and southern territories. Among the sectors reliant on this resource, irrigation stands out as the largest consumer of water. When surface water becomes inaccessible due to insufficient precipitation or other factors, the use of groundwater becomes the only viable alternative for irrigation. The Remila Plain (Khenchela) is located in an endorean watershed in northeastern Algeria and extends over 250 km2 in a synclinal basin filled with water from the Mio-Plio Quaternary - the main aquifer of the region, widely used for irrigation. The aim of this work is to study the hydrochemistry of these waters, as well as the evolution of mineralisation, the identification of the origin of the chemistry, and the suitability of these waters for irrigation. Initial results indicate an evolution of mineralisation in the direction of groundwater flow, with electrical conductivity values varying between 1000µS/cm in the recharge zones, and 2700µS/cm at the outlet. This mineralisation is mainly due to the dissolution of evaporitic minerals and the alteration of silicates. In addition, the various water quality indices used indicate that the water can be used for irrigation without major risk to plants and soils. [ABSTRACT FROM AUTHOR]

Unprecedented plastic production has resulted in over six billion tons of harmful waste. Certain insect taxa emerge as potential agents of plastic biodegradation. Through a comprehensive manual and bibliometric literature analysis, this review analyses and consolidates the growing literature related to insect-mediated plastic breakdown. Over 23 insect species, representing Coleoptera, Lepidoptera, and 4 other orders, have been identified for their capacity to consume plastic polymers. Natural and synthetic polymers exhibit high-level similarities in molecular structure and properties. Thus, in conjunction with comparative genomics studies, we link plastic-degrading enzymatic capabilities observed in certain insects to the exaptation of endogenous enzymes originally evolved for digesting lignin, cellulose, beeswax, keratin and chitin from their native dietary substrates. Further clarification is necessary to distinguish mineralisation from physicochemical fragmentation and to differentiate microbiome-mediated degradation from direct enzymatic reactions by insects. A bibliometric analysis of the exponentially growing body of literature showed that leading research is emerging from China and the USA. Analogies between natural and synthetic polymer's degradation pathways will inform engineering robust enzymes for practical plastic bioremediation applications. By aggregating, analysing, and interpreting published insights, this review consolidates our mechanistic understanding of insects as a potential natural solution to the escalating plastic waste crisis. [ABSTRACT FROM AUTHOR]

This study provides an in-depth geophysical interpretation of the gold and base metal mineralisation of the Ibaga area related to hydrothermal alteration using high-resolution aero-geophysical and induced polarisation ground survey datasets. Despite its potential for gold and base metal and the available artisanal mines, the Ibaga area has remained poorly understood on geophysical signatures of ore deposits and other subsurface geological structures controlling the mineralisation. The magnetic results on the major structures show high magnetic signatures, supported by gravity, radiometric signatures and petrophysical analysis. The potential mineralised structures are interpreted as dyke for gold, and contacts for copper and the depth to the basement varies from 0.113 to 1.556 km. The gravity anomaly shows high values on the gold mineralisation similar to high-density values of dolerite dykes and gabbro and low to medium radiometric concentrations associated with mineralised zones. The magnetic signatures on the copper deposits coincide with the relative positive gravity anomaly of high-density intrusive bodies and relatively moderate conductive bodies with elevated potassium (K) alteration. The copper deposit also has intermediate K and low to intermediate K/Th and K/U ratios caused by K alteration within the geological structures. The good spatial correlation of the hydrothermally altered zones, igneous intrusions and geological structures implies that the geological features associated with gold and copper mineralisation lie within the regions of high-density intrusive bodies and geological structures (faults, fractures and dykes). These features are also associated with relatively moderate conductive bodies, low to intermediate radiometric concentrations and low to moderate magnetic signatures. Thus, the study reveals that the delineated geophysical signatures on gold and base metal mineralisation are associated with quartz–sericite–muscovite ± chlorite hydrothermally altered areas, deformed structures, and intrusive bodies, which act as pathways for the migration and accumulation of hydrothermally altered fluids. [ABSTRACT FROM AUTHOR]

This field study records that the Tortonian phosphatic hardground at S'Algar, Menorca has a diverse fossil macrobenthic fauna dominated by bivalves. These faunal elements occur in four preservational forms (A–D). Each taphonomic form within the hardground is characterised by different concentrations of Entobia isp. (clionaid sponge boring) and encrustation. The preservational forms have specific fossil macrofaunas associated with them, and the hardground represents a melange of bioclasts derived from different environments on the Menorcan Miocene shelf. These habitats are influenced by the upwelling event that provides the conditions for hardground mineralisation. A Glycymeris - Crassatella - Clypeaster association (A) records part of a fauna that lived on the upper ramp slope that was periodically affected by high energy and low oxygen conditions due to upwelling waters. The smaller sized fauna (B), characterised by an arcid-carditid-venerid association is derived from the middle ramp that was affected by storms and a more persistent westward flowing current that intermittently drove upwelling. The fragmentary and disarticulated nature of both faunas indicates that they were mixed within the hardground via reworking processes driven by these seafloor energy regimes. Some of the large gastropod taxa, dentition-up Glycymeris and Gastrochaena suggest that the hardground had its own fauna. Preservation A fauna and other opportunistic taxa inhabited an environment where the hardground was exposed on the Miocene seafloor as 'islands' surrounded by thin layers of looser sediment. Phosphatic bioclasts in the underlying limestone indicate that similar mineralised beds were formed elsewhere on the shelf prior to the formation of the studied outcrop. [ABSTRACT FROM AUTHOR]

Tendon calcification is a commonly associated with degenerative tendinopathy of the Achilles tendons in dogs. It is characterised by the formation of calcific deposits and is refractory to treatment, often re-forming after surgical removal. Little is known about its pathogenesis and therefore the aims of this study were to develop an in vitro model of canine tendon calcification and use this model to investigate mechanisms driving calcification. Cells from the canine Achilles tendon were cultured with different calcifying media to establish which conditions were best able to induce specific, cell-mediated calcification. Once optimum calcification conditions had been established, the effect of ATP treatment on calcification was assessed. Results revealed that 2 mM di-sodium phosphate combined with 2 mM calcium chloride provided the optimum calcifying conditions, increasing calcium deposition and expression of osteogenic-related genes similar to those observed in tendon calcification in vivo. ATP treatment inhibited calcification in a dose-dependent manner, reducing calcium deposition and increasing cell viability, while osteogenic-related genes were no longer upregulated. In conclusion, the in vitro model of canine tendon calcification developed in this study provides the ability to study mechanisms driving tendon calcification, demonstrating that ATP plays a role in modulating tendon calcification that should be explored further in future studies. [ABSTRACT FROM AUTHOR]

One of the deficits of knowledge on bone remodelling, is to what extent cells that are driven towards osteogenic differentiation can contribute to osteoclast formation. The periodontal ligament fibroblast (PdLFs) is an ideal model to study this, since they play a role in osteogenesis, and can also orchestrate osteoclastogenesis.when co-cultured with a source of osteoclast-precursor such as peripheral blood mononuclear cells (PBMCs). Here, the osteogenic differentiation of PdLFs and the effects of this process on the formation of osteoclasts were investigated. PdLFs were obtained from extracted teeth and exposed to osteogenic medium for 0, 7, 14, or 21 out of 21 days. After this 21-day culturing period, the cells were co-cultured with peripheral blood mononuclear cells (PBMCs) for an additional 21 days to study osteoclast formation. Alkaline phosphatase (ALP) activity, calcium concentration, and gene expression of osteogenic markers were assessed at day 21 to evaluate the different stages of osteogenic differentiation. Alizarin red staining and scanning electron microscopy were used to visualise mineralisation. Tartrate-resistant acid phosphatase (TRAcP) activity, TRAcP staining, multinuclearity, the expression of osteoclastogenesis-related genes, and TNF-α and IL-1β protein levels were assessed to evaluate osteoclastogenesis. The osteogenesis assays revealed that PdLFs became more differentiated as they were exposed to osteogenic medium for a longer period of time. Mineralisation by these osteogenic cells increased with the progression of differentiation. Culturing PdLFs in osteogenic medium before co-culturing them with PMBCs led to a significant decrease in osteoclast formation. qPCR revealed significantly lower DCSTAMP expression in cultures that had been supplemented with osteogenic medium. Protein levels of osteoclastogenesis stimulator TNF-α were also lower in these cultures. The present study shows that the osteogenic differentiation of PdLFs reduces the osteoclastogenic potential of these cells. Immature cells of the osteoblastic lineage may facilitate osteoclastogenesis, whereas mature mineralising cells may suppress the formation of osteoclasts. Therefore, mature and immature osteogenic cells may have different roles in maintaining bone homeostasis.

To meet temperature goals that limit warming to well below 2 °C requires the removal of hundreds of billions of tonnes of CO2 from the atmosphere over the course of this century. Effective Carbon Dioxide Removal (CDR) methodologies will be required to reduce net emissions in the near term, counterbalance residual CO2 emissions to achieve net-zero in the medium term, and contribute to net-negative emissions in the longer term – all of this in a sustainable and safe manner. This paper summarizes the research objectives and selected initial results of a collaborative project to assess CO2 storage in the upper ocean crust south of Iceland.The AIMS3 project (www.aims3.cdrmare.de) will deliver new insights, monitoring tools and feasibility assessments for CO2 storage in young, reactive basalts with little sedimentary cover. Along the flank of the Mid-Atlantic Ridge, we have done geophysical surveys and drilled a transect of boreholes in order to identify fluid migration in the upper ocean crust. Both in situ heat flow and geochemical signatures provide irrefutable evidence for such transport, which will help distributing injected CO2 in future experiments.In parallel, our project also has mineralization experiments to assess optimal conditions for injection dissolved, liquid, or supercritical CO2), numerical modelling for upscaling our results from seagoing work, and development of cost-effective sensors and smart robotic landers for long-term monitoring of the vicinity of the boreholes. We outline the rationale of AIMS3, provide an overview of the activities, and highlight some of the expedition results, with the goal to stimulate communication and collaboration.

Large mammalian herbivores substantially impact ecosystem functioning. As their populations are dramatically altered globally, disentangling their consumptive and non‐consumptive effects is critical to advance mechanistic understanding and improve prediction of effects over ecosystem and Earth‐system spatial extents. Mathematical models have played an important role in clarifying potential mechanisms of herbivore zoogeochemistry, based mostly on their consumptive effects as primary consumers and recyclers of organic and inorganic matter via defecation and urination. Trampling is a ubiquitous effect among walking vertebrates, but the consequences and potential mechanisms of trampling in diverse environments remain poorly understood.We derive a novel mathematical model of large mammalian herbivore effects on ecosystem nitrogen cycling, focusing on how trampling and environmental context impact soil processes. We model herbivore trampling with a linear positive or negative additive effect on soil‐mediated nitrogen cycling processes.Combining analytical and numerical analyses, we find trampling by large mammalian herbivores is likely to decrease nitrogen mineralisation rate across diverse environments, such as temperate grassland and boreal forest. These effects are mediated by multiple potential mechanisms, including trampling‐induced changes to detritivore biomass and functioning (e.g. rate of organic matter consumption). We also uncover scenarios where trampling can increase nitrogen mineralisation rate, contingent on the environment‐specific relative sensitivity of detritivore mineral‐nitrogen release and detritivore mortality, to trampling.In contrast to some consumptive mechanisms, our results suggest the pace of soil nitrogen cycling prior to trampling has little influence over the direction of the trampling net effect on nitrogen mineralisation, but that net effects may be greater in slow‐cycling systems (e.g. boreal forests) than in fast‐cycling systems (e.g. grasslands).Our model clarifies the potential consequences of previously overlooked mechanisms of zoogeochemistry that are common to all terrestrial biomes. Our results provide empirically testable predictions to guide future progress in empirical and theoretical studies of herbivore effects in diverse environmental contexts. Resolving ecological contingencies around animal consumptive and non‐consumptive effects will improve whole‐ecosystem management efforts such as restoration and rewilding. [ABSTRACT FROM AUTHOR]

The MC3T3-E1 preosteoblastic cell line is widely utilised as a reliable in vitro system to assess bone formation. However, the experimental growth conditions for these cells hugely diverge, and, particularly, the osteogenic medium (OSM)'s composition varies in research studies. Therefore, we aimed to define the ideal culture conditions for MC3T3-E1 subclone 4 cells with regard to their mineralization capacity and explore if oxidative stress or the cellular metabolism processes are implicated. Cells were treated with nine different combinations of long-lasting ascorbate (Asc) and β-glycerophosphate (βGP), and osteogenesis/calcification was evaluated at three different time-points by qPCR, Western blotting, and bone nodule staining. Key molecules of the oxidative and metabolic pathways were also assessed. It was found that sufficient mineral deposition was achieved only in the 150 μg.mL−1/2 mM Asc/βGP combination on day 21 in OSM, and this was supported by Runx2, Alpl, Bglap, and Col1a1 expression level increases. NOX2 and SOD2 as well as PGC1α and Tfam were also monitored as indicators of redox and metabolic processes, respectively, where no differences were observed. Elevation in OCN protein levels and ALP activity showed that mineralisation comes as a result of these differences. This work defines the most appropriate culture conditions for MC3T3-E1 cells and could be used by other research laboratories in this field. [ABSTRACT FROM AUTHOR]

Wetlands are characterised by soils rich in organic matter that accumulate carbon, providing an important pathway for carbon dioxide sequestration. Nevertheless, not all the carbon fixed can be accumulated, and a proportion will decompose through microbial consumption and be partly released into the atmosphere. Rates of organic matter decomposition in tropical wetlands and the factors associated with this process are scarce. We conducted a 2-year field study in three Melaleuca wetlands in tropical and subtropical Australia using standardised tea litter substrates (green-labile and rooibos-recalcitrant) to measure organic matter decomposition and the microbial communities associated with this process. Decomposition rates were 4-fold higher in labile litter, which was low in carbon: nitrogen, compared to recalcitrant litter. The prokaryotic communities associated with the decomposing litter were unique at each site and different from the soil. They contained taxonomic groups adapted to anaerobic, high temperatures, acidic conditions and suggestive of slow anaerobic turnover. Microbial communities changed as decomposition progressed, with the latter characterised by taxa with cellulose-degrading functions. The decomposition of recalcitrant organic matter within Melaleuca soils was relatively slow, with half of the organic matter inputs remaining after two years, supporting long-term carbon sequestration. [ABSTRACT FROM AUTHOR]

• We examine how earlier 18th and 19th c. antiquaries and scientists mentioned the presence of textile remnants on metal objects • We draw a parallel with contemporary work on a related mechanism, the petrification of wood • We show that the petrification of wood gave rise to experimental archaeology as early as the 16th c. • We discuss how material transformation is influenced by alchemical views of transmutation and imprinting • This conceptualisation has an impact on our contemporary study of mineralised textiles In temperate latitudes, archaeological textiles are rarely found during excavations, except when they have been mineralised in contact with metal artefacts, copper or iron-based. Mineralised textiles are often the only direct source of information on the textile techniques and processes of ancient civilisations. Vivi Sylwan's description of textiles along the Silk Road in the early 20th century is often considered the first detailed archaeological description of mineralised textiles, while the physico-chemist Leo Biek pioneered their microscopic description. In this paper, we examine how antiquaries and scholars from the 18th and the first half of the 19th century identified and studied the presence of textile remains on metal objects, seemingly aware of the significance of these findings. These descriptions share common concepts and vocabulary with writings from the 16th to the early 18th century on petrification of wood. The authors not only show an awareness of and an interest in textile remains, but also question, including experimentally, the process of mineralisation, shaping the modern perception of it with concepts such as "transmutation" or "imprinting". These ancient sources had a lasting impact on our contemporary appraisal of these materials, as we shall discuss in the second stage of this review, which will be devoted to contemporary methods of studying archaeological mineralised textiles. [Display omitted] [ABSTRACT FROM AUTHOR]

The Cobar Basin, in central New South Wales, is an Early Devonian extensional basin that formed in the western Lachlan Orogen. The basin was filled with shallow- to deep-water sequences of the Cobar Supergroup and hosts small to large polymetallic deposits. Detrital zircon geochronology and whole-rock geochemical data collected from the Amphitheatre Group of the Cobar Supergroup provide constraints on the history of basin fill and illustrate the dynamic interplay between basin provenance and mineralisation, corresponding to the evolving tectonic regime. Data reveal provenance dissimilarity between the southern and northern parts of the basin. In the south, units of the Amphitheatre Group received abundant detritus from ca 430–410 Ma magmatic rocks situated to the southwest and southeast of the basin. By contrast, the northern successions were predominately sourced from recycled Ordovician basement found to the northwest, north, northeast of the basin, along with contributions associated with the Macquarie Arc. This spatial provenance variation, however, is less significant in the younger formations: the northern and southern sequences both exhibit an increase in older recycled detritus upwards with time. This reflects a progressive modification of basin paleogeography, during the transition from rift phase to sag phase. The rift-phase basin geography is characterised by fault-restricted deposition with predominant sediments derived from local proximal sources. The subsequent sag-phase subsidence exhibits a uniform depositional system with more homogenised basin input. This provenance variation is coeval with the stratigraphically controlled mineralisation features within the Amphitheatre Group successions, implying a provenance influence on mineralisation. Data suggest the different sediment source regions have produced distinct detrital mineral compositions between the major mineral-hosting and mineral-barren formations. The enrichment of some detrital minerals in the mineral-hosting units, such as feldspar, muscovite, Ti-minerals (and carbonate), is suggested to be an important factor for mineralisation in the basin. Detrital zircon geochronology and whole-rock geochemistry illustrate the history of basin fill for the Cobar Basin. Spatial and temporal variation of basin provenance reflects a modification of basin geography, corresponding to the evolving tectonic regimes. The change in basin source regions is one of key controls on the mineralisation within the Cobar Basin. [ABSTRACT FROM AUTHOR]

Arsenic (As) and cadmium (Cd) are considered toxic elements, even at trace levels. Their accurate quantification in crops can be complex at low levels and due to interference with other elements. The aim of this work was to develop and validate an analytical method for As and Cd quantification in rice stem and grains from the production systems "Irrigated Rice Ecosystems" (IRE) and "Rainfed Rice Ecosystems" (RRE) in Colombia. Mineralisation was carried out by acid digestion using an open system with a heating plate. Metal detection was performed by inductively coupled plasma optical emission spectrometry (ICP‐OES). Method adjustment, calibration, and validation were performed in accordance with AOAC standards, considering sensitivity, precision, accuracy, and selectivity parameters. The obtained method was applied to quantify levels in 259 rice stem and 443 grain samples from IRE and RRE. [ABSTRACT FROM AUTHOR]

This article is an editorial that discusses the evolution of tectonic structures and mineralization in orogens and their margins. Orogens are regions with complex tectonic histories that involve plate subduction, collision, and orogenesis. These processes not only shape the topography but also influence climatic changes and the deposition of mineral resources, particularly precious metals. The article highlights the need for systematic studies of orogens and their nearby margins to improve our understanding of these regions and aid in the exploration of new mineral resources. The editorial also provides a summary of five papers published in this research topic, which cover topics such as magmatism, mineralization, and tectonic evolution in different orogens. [Extracted from the article]

The Ijero-Ekiti tourmaline-bearing pegmatite was investigated, using geological, geochemical, and geophysical methods to characterize its geological evolution and evaluate its mineralisation potential. Petrographical studies revealed a typical granitic mineralogical composition of quartz, feldspar, and muscovite, with tourmaline as accessory minerals. Geochemical data on whole rock analysis showed high and moderate enrichments in SiO2 (av. 71.65 wt. %) and Al2O3 (av. 14.71 wt. %) respectively, while mean values for the other major oxides ranged between 0.01 and 3.63 wt. %. Results also showed that K2O (av. 3.63 wt. %) and Na2O (av. 3.52 wt. %) concentrations were comparable to average Upper Continental values. The relatively high aluminum and alkaline compositions could be attributed to pneumatolytic crystallization from the pegmatitic melt. Aluminum Saturation Index (ASI) [Al/(Ca+Na+K)] indicated peraluminous character. Ranges of some trace elements (Cs, 1.5 - 705.6 ppm; Be, 0.5 - 12 ppm; Sn, 2.0 - 34 ppm; Ta, 1.0 - 14) support a Lithium-Cesium-Tantalum (LCT) type rare-elements pegmatites for the pegmatites of the study. The spider and K/Rb vs K/Cs plots portrayed fractionation trends suggestive of potential mineralisation. The vertical electric sounding data showed that pegmatites underlie the lateritic zone and represent the anomalous layer characterized by high resistivity values ranging from 1086 to 9489 Ohm-m with the surface to a maximum depth of 20.8m which is suspected to be a mineralised zone. Some of these pegmatites are highly tourmalinated, suggesting that they emanate from highly mineralised magma and are believed to represent late-stage pneumatolytic fluids derived from acidic magma bodies through magmatogenic processes. [ABSTRACT FROM AUTHOR]

The study investigated the effect of dietary inclusion of bone dust as a calcium source on performance, egg quality characteristics, serum parameters, blood minerals and bone mineralisation of laying chickens. A total of 128 point of lay birds (1470-1550g) were allotted on a weight equalisation basis to four dietary treatments. Each treatment consists of four replicates having 8 birds per replicate. The diets were designated T1, T2, T3 and T4 in which T1 is the diet with dicalcium phosphate (DCP), T2 is the diet with bone meal, T3 is the diet with 50% bone dust and 50% DCP and T4 is the diet with 100% bone dust. The experiment lasted for eight weeks; laying performance and egg qualities (internal and external) were evaluated on a weekly basis. Serum parameters, blood minerals and bone mineralisation were carried out at the end of the study. Data collected were subjected to a one-way analysis of variance using Minitab statistical software (17.0) and significant means were separated using Tukey’s test. The inclusion of 50% and 100% dust resulted in increased (P<0.05) daily feed intake (DFI). The use of 100% bone dust in the diet of layers resulted in the highest (P<0.05) number of eggs laid (NEL) (293.00) and Hen-day egg production (HDEP) (118.33 %). Eggshell thickness (EST) was highest for layers fed T3 and T4 diets. The highest (P<0.05) albumin height (AH) (10.04 mm) and Haugh unit (Hu) (94.62 %) was observed for layers fed T4 diet. Layers fed T1 and T4 diets had higher (P<0.05) total protein and globulin than other treatments. Calcium and phosphorus content in blood was highest (P<0.05) for layers fed T1 diet. Bone calcium and phosphorus were highest (P<0.05) for layers fed the T3 diet but lowest for those fed the T1 diet. It was concluded that bone dust can be included in the diet of layers at 50 and 100% for better performance and egg quality without negative effects on blood and mineral composition. [ABSTRACT FROM AUTHOR]

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This work aims to study derivative removal of gentamicin (GMC) antibiotic from the aqueous solution within photocatalytic process using a recyclable magnetic titania (MT) photocatalyst. The MT photocatalyst was synthesised, characterised, and utilised by using co-precipitating method. The process of modelling and optimisation was performed by a second-order polynomial model. The 'F-value' of 432.56 and 'P-value' of 0.0001 indicated that the proposed model could be used for simulating the gentamicin photocatalytic removal process. According to the model prediction, the process could remove GMC antibiotic up to 94.7% in the optimum conditions (initial pH = 6.9, [GMC] = 20 ppm, MT dosage = 195 mg/L, and time = 63 min). Recycling and reusing potentials of the synthesised MT catalyst were studied in five cycles in the optimum conditions. According to the obtained results, in the first stage of catalyst recycling, the removal efficiency decreased by about 12%, but in the subsequent stages, the removal efficiency reduction was lower than the first step and about 5%. Mineralisation of the 51.3% efficiency was obtained in the optimum conditions. Kinetic study results showed that the data were well fitted by pseudo-first-order kinetic model with the regression coefficient of 0.9769. Also, the related reaction rate constant was 0.041 min−1. The effect of sulphate and chloride ions, as radical scavengers, on the removal efficiency demonstrated a reduction in GMC removal efficiency, which occurred by using chloride and sulphate ions. The results represented applying MT for treating real wastewater could remove GMC antibiotic in hospital wastewater up to 91.21%, too. [ABSTRACT FROM AUTHOR]

In this study, removal of P-Nitrophenol (PNP) as a nitro-aromatic contaminant from the aqueous medium have been investigated using the photochemical oxidation process by UV-activated periodate. The experiments were performed in a photochemical reactor with a volume of one litre. The central composite design (CCD) as a response surface method was applied for the experimental design and optimisation of the process parameters by design expert software. The aim of this study was to distinguish the optimum level of independent variables intended for the dependent variable. The selected independent variables were the initial concentration of PNP, initial pH, and the amount of initial concentration of KIO4 that could affect the degradation efficiency (DE) as response (dependent variable). The CCD demonstrated an excellent relationship between the independent and dependent variables. The mathematical equations and response surface plots were utilised to determine the interactions and explain the results. The optimum conditions were found to be at pH = 5.8, [KIO4] = 83.3 ppm, [PNP] = 10 ppm, and after 30 min of the process, 88% degradation efficiency was obtained. To verify the adequacy and prediction power of the model, the predicted optimum conditions were validated by the actual experiments. Furthermore, p-nitrophenol mineralisation was also examined under optimal conditions and displayed the ability of the process to mineralise 35% of p-nitrophenol after 30 min. Also electrical energy consumption of the process was obtained about 102.74 kWh/m3. [ABSTRACT FROM AUTHOR]

The permeability of faults and their sedimentary host rocks is a critical input for models of fluid flow in sedimentary basins. Permeability of sedimentary rocks can vary by orders of magnitude over short distances due to variations in sedimentary facies, as well as being strongly anisotropic. Structural features also affect permeability, with faults acting as fluid conduits or barriers depending on the nature of the sedimentary host rock. Constraining these variations in permeability is challenging where outcrops are lacking and drillhole data are sparse. This study describes a workflow using stratigraphic forward modelling to estimate the permeability distribution (both magnitude and anisotropy) in sedimentary rocks and associated faults, which is then used in fluid flow simulations. Permeability is represented as a tensor in the global coordinate system, enabling the use of an unstructured mesh that is independent of stratigraphic layering. The workflow is demonstrated in a sub‐basin of the Proterozoic McArthur Basin of northern Australia. A range of fault permeability scenarios are explored, where fault permeability is a function of host rock properties. The simulation results demonstrate the importance of capturing the direction of permeability anisotropy in dipping strata, as well as highlighting the effect of different fault permeability scenarios. The workflow is particularly well‐suited to scenarios where there are sufficient boreholes to constrain a stratigraphic forward model, but insufficient to determine the permeability distribution by interpolation. Potential applications include mineral and hydrocarbon exploration, groundwater studies, contaminant dispersal modelling, and CO2 sequestration. [ABSTRACT FROM AUTHOR]

For the last 30 years, interest has focused on biochar and its potential to store carbon in soil to mitigate climate change whilst improving soil properties for increased crop production and, therefore, could play a critical role in both agricultural sustainability and broader environmental aims. Biochar, a carbonaceous product, is formed from organic feedstock pyrolysised in the absence of air and, therefore, is a potential means of recycling organic waste. However, different feedstock and pyrolysis conditions result in a biochar with a range of altered characteristics. These characteristics influence nitrogen transformation processes in soil and result in the metabolism of different substrates and the formation of different products, which have different effects on agricultural yield. This paper reviews how the production of biochar, from varying feedstock and pyrolysis conditions, results in different biochar characteristics that influence each stage of the nitrogen cycle, namely processes involved in fixation, assimilation, mineralisation and denitrification. The nitrogen cycle is briefly outlined, providing a structure for the following discussion on influential biochar characteristics including carbon composition (whether recalcitrant or rapidly metabolisable), mineral composition, surface area, porosity, cation exchange capacity, inhibitory substances and pH and so on. Hence, after the addition of biochar to soil, microbial biomass and diversity, soil porosity, bulk density, water-holding capacity, cation exchange capacity, pH and other parameters change, but that change is subject to the type and amount of biochar. Hence, products from soil-based nitrogen transformation processes, which may be beneficial for plant growth, are highly dependent on biochar characteristics. The paper concludes with a diagrammatic summation of the influence of biochar on each phase of the nitrogen cycle, which, it is hoped, will serve as a reference for both students and biochar practitioners. [ABSTRACT FROM AUTHOR]

The extracellular matrix of white adipose tissue (AT) is a very promising biomaterial for tissue engineering, due to its abundance, easy accessibility, malleability, and proven biological activity. Decellularised AT (DAT) can be processed by freeze-drying in acetic acid solutions, and changing the DAT concentration in the solution gives rise to three-dimensional (3D) scaffolds of different stiffness and porosity. In a previous report, we demonstrated that human Dental Pulp Stem Cells (hDPSCs) could differentiate to osteoblasts and generate mineralised bone on 3D solid foams of porcine Decellularised Adipose Tissue (pDAT) at a concentration of 0.5 % (w/v). In this research work, we assessed whether and how osteogenesis by hDPSCs would be regulated by testing pDAT solid foams formulated at three different concentrations: 0.25 % (w/v), 0.5 % (w/v), and 1 % (w/v), which showed different stiffness, porosity and water retention properties. As a control condition we tested solid foams formulated with 0.5 % bovine Collagen-I. Thus, we performed Alkaline Phosphatase and Alizarin Red staining, together with Transmission Electron Microscopy and the detection of osteoblastic differentiation markers Osterix and Osteocalcin at both protein and transcript level, to compare the osteogenesis mediated by hDPSCs grown on all these 3D scaffolds, in the presence or absence of osteoblastic induction media. Our results demonstrate that pDAT at 0.25 % supported osteogenesis better than the rest of tested scaffolds, including bovine Collagen-I, in 3D hDPSC cultures. This enhanced osteogenesis could be attributed to the formulation of 0.25 % pDAT solid foams, which presented a higher porosity.

Abstract The significant impact of vegetation restoration on the turnover of organic carbon (OC) is mainly attributed to biological processes. However, the contribution of abiotic processes associated with reactive oxygen species (ROS) during vegetation restoration has remained largely overlooked. Therefore, we systematically explored ROS distribution and production mechanisms in sandy soil and their corresponding effects on OC oxidation through field monitoring and incubation experiments. The cumulative concentrations of hydroxyl radicals (•OH) produced within 24 h varied in different soils undergoing vegetation restoration, ranging from 2.36 to 22.05 μmol kg‒1, which were derived from the Fenton-like reaction of hydrogen peroxide (H2O2) and biotic-induced Fe(II). In addition, during the oxidation of reductive SOC, H2O2 can be formed and react with Fe(II) to generate •OH. Further, •OH significantly induced the abiotic transformation of OC, accounting for 15.93%‒25.80% of carbon dioxide (CO2) efflux. Specifically, 1 mol •OH can induce the production of 0.01–0.02 mol CO2 for particulate organic carbon (POC) and 0.03‒0.23 mol CO2 for mineral-associated organic carbon (MOC), as demonstrated by chemical quenching and sterilisation experiments. This indicated that MOC was more sensitive to •OH attacks. Collectively, our findings provide insights into the accumulation of ROS in sandy soils during vegetation restoration, with •OH playing a key role in SOC oxidation, as well as the patterns of SOC turnover and its response to changes in the soil environment. Graphical Abstract

This study presents an analysis of aeromagnetic and aero-radiometric data from the Chitipa area in northern Malawi to delineate structures, hydrothermal alteration areas, and gold mineral potential zones, as well as to identify prospective regions for further mineral exploration. Airborne geophysical data, specifically aeromagnetic and aero-radiometric data, were utilized. We applied several enhancements and filters to the geophysical data, including reduction to the pole, the first vertical derivatives, analytical signal, tilt angle derivative enhancements, Centre for Exploration Targeting (CET) grid analysis, Euler deconvolution, and radiometric data ratios. The results of the analysis provided detailed information on the subsurface geology and indicate that the area is characterized by faulting and shearing with structures predominantly trending in a northwest direction, and minor trends in the northeast-southwest, east-west, and north-south directions. Zones with hydrothermal alteration were found to coincide with structural associations in the NW part of the study area, indicating that the structures served as channel ways for migrating hydrothermal fluids that reacted with the rock formation, resulting in alteration. The northwest area is a promising mineralization zone, and further exploration should focus on this area.

Per- and polyfluoroalkyl substances (PFAS) are a group of fluorinated synthetic chemicals that are highly recalcitrant, toxic, and bio-accumulative and have been detected in biosolids worldwide, posing potential risks to humans and the environment. Recent studies suggest that the organic C-F bond in PFAS can be destructed and potentially mineralised into inorganic fluorides during thermal treatment. This study focuses on thermodynamic equilibrium investigations and the fate of fluorine compounds post-PFAS destruction during biosolid thermal treatment. The results indicate that gas-phase fluorine compounds are mainly hydrogen fluoride (HF) and alkali fluorides, whereas solid-phase fluorine compounds include alkaline earth fluorides and their spinels. High moisture and oxygen content in the volatiles increased the concentration of HF in the gas phase. However, adding minerals reduced the emission of HF in the gas phase significantly and enhanced the capture of fluorine as CaF2 spinel in the solid phase. This study also investigates the effect of feedstock composition on the fate of fluorine. High ash content and low volatile matter in the feedstock reduced HF gas emissions and increased fluorine capture in the solid product. The findings of this work are useful in designing thermal systems with optimised operating conditions for minimising the release of fluorinated species during the thermal treatment of PFAS-containing biosolids.

Unprecedented plastic production has resulted in over six billion tons of harmful waste. Certain insect taxa emerge as potential agents of plastic biodegradation. Through a comprehensive manual and bibliometric literature analysis, this review analyses and consolidates the growing literature related to insect-mediated plastic breakdown. Over 23 insect species, representing Coleoptera, Lepidoptera, and 4 other orders, have been identified for their capacity to consume plastic polymers. Natural and synthetic polymers exhibit high-level similarities in molecular structure and properties. Thus, in conjunction with comparative genomics studies, we link plastic-degrading enzymatic capabilities observed in certain insects to the exaptation of endogenous enzymes originally evolved for digesting lignin, cellulose, beeswax, keratin and chitin from their native dietary substrates. Further clarification is necessary to distinguish mineralisation from physicochemical fragmentation and to differentiate microbiome-mediated degradation from direct enzymatic reactions by insects. A bibliometric analysis of the exponentially growing body of literature showed that leading research is emerging from China and the USA. Analogies between natural and synthetic polymer’s degradation pathways will inform engineering robust enzymes for practical plastic bioremediation applications. By aggregating, analysing, and interpreting published insights, this review consolidates our mechanistic understanding of insects as a potential natural solution to the escalating plastic waste crisis.

The improvement of rice production to meet food needs for the increasing population is a general problem faced in wetland development for agriculture. The use of industrial waste, such as coal fly ash (CFA), could effectively improve the soil properties of wetlands. In this study, CFA with an amount of 2% (weight/volume) or 240 g was added to 12 L of three different soils collected from the rice fields (peatland, swampland, and rainfed field) in a 15-L pot, and then incubated in the greenhouse for 15 days. The soil pH, concentrations of NH4+-N, NO3--N, exchangeable calcium (Ca) and magnesium (Mg) and available phosphorus in the soil were quantified following the completion of the incubation. Rice seedlings were planted in each pot, and after 90 days, the growth and yield variables were observed. The results showed that CFA application enhanced the concentrations of NH4+-N, NO3--N, and available phosphorus in peatland and swampland, the rice fields that contain high organic carbon (C), which ultimately leads to increasing rice growth and yield. The application of CFA to rice fields containing low organic carbon did not improve available nitrogen and phosphorus nor enhance the growth and yield of rice. Results of this study indicate an important role of soil organic C content in the rice fields in controlling the effect of CFA on nutrient availability, growth and yield of rice.

The purpose of the study is to develop a novel peptide for caries management. Gallic-Acid-Polyphemusin-I (GAPI) was synthesised by grafting Polyphemusin I (PI) and gallic acid (GA). Biocompatibility was evaluated using a Cell Counting Kit-8 Assay. Antimicrobial properties were assessed using minimum inhibitory concentration (MIC) and minimum bactericidal/fungicidal concentration (MBC/MFC). The bacterial and fungal morphology after GAPI treatment was investigated using transmission electron microscopy (TEM). The architecture of a consortium biofilm consisting of Streptococcus mutans, Lacticaseibacillus casei and Candida albicans was evaluated using scanning electron microscopy (SEM) and confocal laser scanning microscopy. The growth kinetics of the biofilm was examined using a propidium monoazide–quantitative polymerase chain reaction. The surface and calcium-to-phosphorus molar ratio of GAPI-treated enamel after pH cycling were examined with SEM and energy-dispersive X-ray spectroscopy. Enamel crystal characteristics were analysed using X-ray diffraction. Lesion depths representing the enamel's mineral loss were assessed using micro-computed tomography. The MIC of GAPI against S. mutans, L. casei and C. albicans were 40 μM, 40 μM and 20 μM, respectively. GAPI destroyed the biofilm's three-dimensional structure and inhibited the growth of the biofilm. SEM showed that enamel treated with GAPI had a relatively smooth surface compared to that treated with water. The calcium-to-phosphorus molar ratio of enamel treated with GAPI was higher than that of the control. The lesion depths and mineral loss of the GAPI-treated enamel were less than the control. The crystallinity of the GAPI-treated enamel was higher than the control. This study developed a biocompatible, mineralising and antimicrobial peptide GAPI, which may have potential as an anti-caries agent. [ABSTRACT FROM AUTHOR]

The significant impact of vegetation restoration on the turnover of organic carbon (OC) is mainly attributed to biological processes. However, the contribution of abiotic processes associated with reactive oxygen species (ROS) during vegetation restoration has remained largely overlooked. Therefore, we systematically explored ROS distribution and production mechanisms in sandy soil and their corresponding effects on OC oxidation through field monitoring and incubation experiments. The cumulative concentrations of hydroxyl radicals (•OH) produced within 24 h varied in different soils undergoing vegetation restoration, ranging from 2.36 to 22.05 μmol kg‒1, which were derived from the Fenton-like reaction of hydrogen peroxide (H2O2) and biotic-induced Fe(II). In addition, during the oxidation of reductive SOC, H2O2 can be formed and react with Fe(II) to generate •OH. Further, •OH significantly induced the abiotic transformation of OC, accounting for 15.93%‒25.80% of carbon dioxide (CO2) efflux. Specifically, 1 mol •OH can induce the production of 0.01–0.02 mol CO2 for particulate organic carbon (POC) and 0.03‒0.23 mol CO2 for mineral-associated organic carbon (MOC), as demonstrated by chemical quenching and sterilisation experiments. This indicated that MOC was more sensitive to •OH attacks. Collectively, our findings provide insights into the accumulation of ROS in sandy soils during vegetation restoration, with •OH playing a key role in SOC oxidation, as well as the patterns of SOC turnover and its response to changes in the soil environment. Highlights: • Vegetation restoration exerts a regulatory influence on ROS production in sandy soil. • ROS accumulation in tree-covered soil is more than that in shrub-covered soil. • 15.93%‒25.80% of total CO2 efflux in sandy soils is from •OH mediated OC mineralisation. • Mineral-associated OC exhibits a higher susceptibility to •OH attack than particulate OC. [ABSTRACT FROM AUTHOR]

Every nonlinear system grows by increments, and the final probability distributions for components of that system emerge from an amalgamation of these increments. The resulting probability distribution depends on the constraints imposed on each increment by the physical and chemical processes that produce the system. Hence there is the potential that the observed probability distribution can reveal information on these processes. Complex systems that grow by competition between the supply and consumption of energy and mass have growth laws that are cumulative probability distributions for their component parts that reflect such competition. We show that the type of probability distribution is characteristic of the endowment of orogenic gold deposits with the sequence: Weibull → Fréchet → gamma → log normal representative of increasing endowment. Further, the differential entropy of the probability distribution is indicative of the quality of the deposit, with low-quality deposits represented by high entropy and high-quality deposits represented by low or negative entropy. The type of probability distribution gives an indication of the processes that operated to produce the deposit. These conclusions hold for mineralisation as well as for the associated alteration assemblages. We suggest that the probability distribution for the mineralisation or the alteration assemblage gives a good indication of the endowment and quality of a deposit from a single drill hole. A single drill hole from a deposit can provide information on endowment and organisation. Weibull → Fréchet → gamma → log normal probability distributions are representative of increasing gold endowment. The differential entropies of these distributions characterise the organisation of the system. [ABSTRACT FROM AUTHOR]

This paper focuses on the geology and geochemistry of the Rhyacian Josephine gold deposit in northwestern Ghana, where gold is primarily hosted in quartzites, with other lithologies observed, including granitoids, mafic intrusions, and gneisses. Four deformational events, DJO0, DJO1, DJO2, and DJO3, are observed. DJO0 is synonymous with the primary preserved sedimentary crossbedding in the quarzitic rocks. Gold mineralization within the deposit is mainly associated with the DJO1 NNW-trending dextral and steeply dipping sinusoidal shear zone. Gold occurs primarily as free gold and invisible gold within the lattices of the disseminated arsenopyrite along the shear foliation planes of the host rock. The ore body geometry is 15-40 m wide and strikes 800 m in the NNW direction. Silicification, sericitization, chloritization, and sulphidation are the main alteration assemblages associated with the gold mineralization zones within the Josephine deposit. Pathfinder elements for gold are stibnite, tellurium, sulphur, copper, silver, and Lead. [ABSTRACT FROM AUTHOR]

An analysis of aeromagnetic and aero-radiometric data from the Chitipa area in northern Malawi is presented in this study. The aim is to delineate structures, hydrothermal alteration areas, and gold mineral potential zones, as well as to identify regions suitable for further mineral exploration. Airborne geophysical data, specifically aeromagnetic and aero-radiometric data, were employed. Several enhancement techniques and filters were applied to the geophysical data, including reduction to the pole, computation of the first vertical derivatives, analytical signal processing, tilt angle derivative enhancements, Centre for Exploration Targeting (CET) grid analysis, Euler deconvolution, and radiometric data ratios. The results of the analysis offer detailed insights into the subsurface geology. It is indicated that the area is characterized by faulting and shearing, with structures predominantly trending in a northwest direction. Minor trends in the northeast-southwest, east-west, and northsouth directions were also observed. Zones with hydrothermal alteration were discovered to coincide with structural associations in the NW part of the study area, suggesting that the structures served as channels for migrating hydrothermal fluids that reacted with the rock formation, resulting in alteration. The northwest area is identified as a promising mineralization zone, emphasizing the need for further exploration efforts in this region. [ABSTRACT FROM AUTHOR]

The relationship between carbon dioxide (CO2) production in soil respiration and soil carbon (C) content was studied using soil samples from agricultural field parcels where the C content changed along a transect within the field. Incubation of soil samples from 30 sampling points within five fields showed increasing CO2 production with rising soil C content within the range 3–49 %. The amount of CO2 formed in relation to the C content (specific respiration) decreased as the soil C content increased. Thus, diminishing C content of the peat as time passes after drainage does not necessarily lead to proportionally lower emissions and, indeed, our results suggest that the vulnerability of the organic matter to decomposition increases with time since drainage. When divided into classes of mineral soils (0–12 % C), mull soils (12–23 % C) and peat soils (> 23 % C) according to the Finnish national classification, only the mineral and peat soils differed from each other with respect to respiration rate. These results support including mull soils with peat soils when estimating the emissions from organic soils for greenhouse gas inventories. It is also evident that CO2 emissions from some soils classified as mineral soils can be comparable to emissions from organic soils.

Soil organic matter can be divided into stable and labile, with the latter directly supporting plant growth and development during the growing season. To restore soil fertility and ensure high productivity of agrocenoses, a certain ratio between the labile and stable parts of humus must be maintained. The purpose of this study was to determine the content of labile organic matter under different tillage and fertilisation options and to establish the optimal ratio between their content and the content of total carbon, based on which to develop measures to restore and improve the fertility of chernozem soils. In stationary experiments, the study investigated the impact of the intensity of chernozem use, its cultivation and fertilisation systems on the humus state of the amount of labile organic matter and its ratio to humus carbon. Field research, laboratory method of soil sample analysis, systematisation, analysis, and generalisation were used in the study. The soil samples were analysed for humus content according to DSTU 4289:2004 and labile organic matter content according to DSTU 4732:2007. When chernozems are ploughed up, labile organic matter is mineralised the most and their share in humus decreases, while when arable land is converted to fallow land, it is restored the fastest. Minimising tillage systems and applying organic and mineral fertilisers increases the amount of labile organic matter in the humus of chernozems. The use of no-till for sixteen years resulted in a 0.13% increase in humus content, and a 0.53% increase over forty-two years compared to ploughing. The application of organo-mineral fertiliser increased the ratio of labile organic matter carbon content to total carbon content compared to the unfertilised variants. A strong correlation was found between the content of labile organic matter in chernozems and the yield of winter wheat and maize. The practical significance of the research results is to minimise the fertiliser rate and the C:N ratio to ensure gross crop yields and restore soil fertility

The MC3T3-E1 preosteoblastic cell line is widely utilised as a reliable in vitro system to assess bone formation. However, the experimental growth conditions for these cells hugely diverge, and, particularly, the osteogenic medium (OSM)’s composition varies in research studies. Therefore, we aimed to define the ideal culture conditions for MC3T3-E1 subclone 4 cells with regard to their mineralization capacity and explore if oxidative stress or the cellular metabolism processes are implicated. Cells were treated with nine different combinations of long-lasting ascorbate (Asc) and β-glycerophosphate (βGP), and osteogenesis/calcification was evaluated at three different time-points by qPCR, Western blotting, and bone nodule staining. Key molecules of the oxidative and metabolic pathways were also assessed. It was found that sufficient mineral deposition was achieved only in the 150 μg.mL−1/2 mM Asc/βGP combination on day 21 in OSM, and this was supported by Runx2, Alpl, Bglap, and Col1a1 expression level increases. NOX2 and SOD2 as well as PGC1α and Tfam were also monitored as indicators of redox and metabolic processes, respectively, where no differences were observed. Elevation in OCN protein levels and ALP activity showed that mineralisation comes as a result of these differences. This work defines the most appropriate culture conditions for MC3T3-E1 cells and could be used by other research laboratories in this field.

Earthworms can increase the solubility of phosphorus (P) in soil, an effect which to a large extent is controlled by mineralisation of organic P. Phosphatases, a class of hydrolytic enzymes, catalyse this mineralisation process. However, a consistent comparison of their activity among earthworm species and for different soil spheres is still missing. Here we aim to better understand the activity of phosphatases in relation to earthworm-enhanced P-availability, and specifically that of phosphomonoesterases (PME) which directly liberate orthophosphate. We conducted a greenhouse pot experiment with five different earthworm species. The PME activity was assessed in earthworm casts, burrows and bulk soil for both single species and mixed communities. Analyses were performed at both the pH-H2O of the bulk soil (6.5) and of the casts (7.5). The PME activity measured at both pH values was highly correlated (R2 = 0.98; p = 2.2x10-16) and was strongly elevated by earthworm activity in the order cast > burrows > bulk soil. The PME activity was particularly high in the casts of A. longa relative to casts of other species. Large variation in PME activity in the drilosphere was observed among earthworm species, but this variation was not related to earthworm ecological categories. Our data also indicate that an elevated P concentration in grass shoots could result from increased PME activity via greater hydrolysis of organic P.