1,934 results on '"Min, Peng"'
Search Results
2. Sortilin-mediated translocation of mitochondrial ACSL1 impairs adipocyte thermogenesis and energy expenditure in male mice
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Min Yang, Jing Ge, Yu-Lian Liu, Huan-Yu Wang, Zhi-Han Wang, Dan-Pei Li, Rui He, Yu-Yu Xie, Hong-Yan Deng, Xue-Min Peng, Wen-She Wang, Jia-Dai Liu, Zeng-Zhe Zhu, Xue-Feng Yu, Pema Maretich, Shingo Kajimura, Ru-Ping Pan, and Yong Chen
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Science - Abstract
Abstract Beige fat activation involves a fuel switch to fatty acid oxidation following chronic cold adaptation. Mitochondrial acyl-CoA synthetase long-chain family member 1 (ACSL1) localizes in the mitochondria and plays a key role in fatty acid oxidation; however, the regulatory mechanism of the subcellular localization remains poorly understood. Here, we identify an endosomal trafficking component sortilin (encoded by Sort1) in adipose tissues that shows dynamic expression during beige fat activation and facilitates the translocation of ACSL1 from the mitochondria to the endolysosomal pathway for degradation. Depletion of sortilin in adipocytes results in an increase of mitochondrial ACSL1 and the activation of AMPK/PGC1α signaling, thereby activating beige fat and preventing high-fat diet (HFD)-induced obesity and insulin resistance. Collectively, our findings indicate that sortilin controls adipose tissue fatty acid oxidation by substrate fuel selection during beige fat activation and provides a potential targeted approach for the treatment of metabolic diseases.
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- 2024
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3. IRF8 deficiency-induced myeloid-derived suppressor cell promote immune evasion in lung adenocarcinoma
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Zhen Gao, Shang Liu, Han Xiao, Meng Li, Wan-gang Ren, Lin Xu, and Zhong-min Peng
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Lung adenocarcinoma ,MDSCs ,IRF8 ,Tumor microenvironment ,Immune escape ,Medicine - Abstract
Abstract Background Patients with lung adenocarcinoma (LUAD) have a low response rate to immune checkpoint blockade. It is highly important to explore the tumor immune escape mechanism of LUAD patients and expand the population of patients who may benefit from immunotherapy. Methods Based on 954 bulk RNA-seq data of LUAD patients and 15 single-cell RNA-seq data, the relationships between tumor immune dysfunction and exclusion (TIDE) scores and survival prognosis in each patient were calculated and evaluated, and the immune escape mechanism affecting the independent prognosis of LUAD patients was identified. Functional enrichment analysis explored the antitumour immune response and biological behavior of tumor cells among different LUAD groups. Single-cell annotation and pseudotemporal analysis were used to explore the target molecules and immune escape mechanisms of LUAD. Results Myeloid-derived suppressor cells (MDSCs) and IRF8 were identified as risk and protective factors for the independent prognosis of LUAD patients, respectively. In the tumor microenvironment of patients with high infiltration of MDSCs, the antitumor immune response is significantly suppressed, while tumor cell division, proliferation, and distant metastasis are significantly enhanced. Single-cell RNA-seq analysis revealed that IRF8 is an important regulator of MDSC differentiation in LUAD myeloid cells. In addition, IRF8 may regulate the differentiation of MDSCs through the IL6-JAK-STAT3 signalling pathway. Conclusions IRF8 deficiency impairs the normal development of LUAD myeloid cells and induces their differentiation into MDSCs, thereby accelerating the immune escape of LUAD cells. IRF8-targeted activation to inhibit the formation of MDSCs may be a new target for immunotherapy in LUAD.
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- 2024
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4. The impact of tertiary lymphoid structures on tumor prognosis and the immune microenvironment in non-small cell lung cancer
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Yiming Weng, Jingping Yuan, Xue Cui, Jinsong Wang, Honglei Chen, Li Xu, Xinyi Chen, Min Peng, and Qibin Song
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Non-small cell lung cancer (NSCLC) ,Tertiary lymphoid structures (TLSs) ,Immunotherapy ,Prognosis ,Tumor microenvironment (TME) ,Medicine ,Science - Abstract
Abstract Non-small cell lung cancer (NSCLC) is a common malignancy whose prognosis and treatment outcome are influenced by many factors. Some studies have found that tertiary lymphoid structures (TLSs) in cancer may contribute to prognosis and the prediction of immunotherapy efficacy However, the combined role of TLSs in NSCLC remains unclear. We accessed The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases to obtain mRNA sequencing data and clinical information as the TCGA cohort, and used our own sample of 53 advanced NSCLC as a study cohort. The samples were divided into TLS+ and TLS- groups by pathological tissue sections. Patients of the TLS+ group had a better OS (p = 0.022), PFS (p = 0.042), and DSS (p = 0.004) in the TCGA cohort, and the results were confirmed by the study cohort (PFS, p = 0.012). Furthermore, our result showed that the count and size of TLSs are closely associated with the efficacy of immunotherapy. In addition, the TLS+ group was associated with better immune status and lower tumor mutation load. In the tumor microenvironment (TME), the expression levels of CD4+ T cells and CD8+ T cells of different phenotypes were associated with TLSs. Overall, TLSs are a strong predictor of survival and immunotherapeutic efficacy in advanced NSCLC, and T cell-rich TLSs suggest a more ordered and active immune response site, which aids in the decision-making and application of immunotherapy in the clinic.
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- 2024
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5. SHR-A1811 (antibody-drug conjugate) in advanced HER2-mutant non-small cell lung cancer: a multicenter, open-label, phase 1/2 study
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Ziming Li, Zhengbo Song, Wei Hong, Nong Yang, Yongsheng Wang, Hong Jian, Zibin Liang, Sheng Hu, Min Peng, Yan Yu, Yan Wang, Zicong Jiao, Kaijing Zhao, Ke Song, You Li, Wei Shi, and Shun Lu
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Medicine ,Biology (General) ,QH301-705.5 - Abstract
Abstract A dose-escalation and expansion, phase 1/2 study (ClinicalTrials.gov, NCT04818333) was conducted to assess the novel antibody-drug conjugate SHR-A1811 in pretreated HER2-altered advanced non-small cell lung cancer (NSCLC). Here, we report results from the phase 1 portion. Patients who had previously failed or were intolerant to platinum-based chemotherapy were enrolled and received SHR-A1811 intravenously at doses of 3.2 to 8.0 mg/kg every 3 weeks. Dose escalation followed a Bayesian logistic regression model that included overdose control, with subsequent selection of tolerable levels for dose expansion. Overall, 63 patients were enrolled, including 43 receiving a recommended dose for expansion of 4.8 mg/kg. All patients had HER2-mutant disease. Dose-limiting toxicity occurred in one patient in the 8.0 mg/kg dose cohort. Grade ≥ 3 treatment-related adverse events occurred in 29 (46.0%) patients. One patient in the 6.4 mg/kg cohort died due to interstitial lung disease. As of April 11, 2023, the 4.8 mg/kg cohort showed an objective response rate of 41.9% (95% CI 27.0–57.9), and a disease control rate of 95.3% (95% CI 84.2–99.4). The median duration of response was 13.7 months, with 13 of 18 responses ongoing. The median progression-free survival was 8.4 months (95% CI 7.1–15.0). SHR-A1811 demonstrated favourable safety and clinically meaningful efficacy in pretreated advanced HER2-mutant NSCLC.
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- 2024
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6. Biochemical indicators, cell apoptosis, and metabolomic analyses of the low-temperature stress response and cold tolerance mechanisms in Litopenaeus vannamei
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Weilin Zhu, Qiangyong Li, Min Peng, Chunling Yang, Xiuli Chen, Pengfei Feng, Qingyun Liu, Bin Zhang, Digang Zeng, and Yongzhen Zhao
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Litopenaeus vannamei ,Metabolomics ,Cell apoptosis ,Low-temperature stress ,Biochemical indicators ,Medicine ,Science - Abstract
Abstract The cold tolerance of Litopenaeus vannamei is important for breeding in specific areas. To explore the cold tolerance mechanism of L. vannamei, this study analyzed biochemical indicators, cell apoptosis, and metabolomic responses in cold-tolerant (Lv-T) and common (Lv-C) L. vannamei under low-temperature stress (18 °C and 10 °C). TUNEL analysis showed a significant increase in apoptosis of hepatopancreatic duct cells in L. vannamei under low-temperature stress. Biochemical analysis showed that Lv-T had significantly increased levels of superoxide dismutase (SOD) and triglycerides (TG), while alanine aminotransferase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH-L), and uric acid (UA) levels were significantly decreased compared to Lv-C (p
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- 2024
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7. Dólares or Dollars? Unraveling the Bilingual Prowess of Financial LLMs Between Spanish and English.
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Xiao Zhang, Ruoyu Xiang, Chenhan Yuan, Duanyu Feng, Weiguang Han, Alejandro Lopez-Lira, Xiao-Yang Liu, Meikang Qiu, Sophia Ananiadou, Min Peng 0002, Jimin Huang, and Qianqian Xie
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- 2024
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8. HealMe: Harnessing Cognitive Reframing in Large Language Models for Psychotherapy.
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Mengxi Xiao, Qianqian Xie, Ziyan Kuang, Zhicheng Liu, Kailai Yang, Min Peng 0002, Weiguang Han, and Jimin Huang
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- 2024
9. UniLP: Unified Topology-aware Generative Framework for Link Prediction in Knowledge Graph.
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Ben Liu, Miao Peng, Wenjie Xu, Xu Jia, and Min Peng
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- 2024
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10. Safety and efficacy of immune checkpoint inhibitor rechallenge in advanced non-small cell lung cancer: a retrospective study
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Feng, Jia, Chen, Xinyi, Wei, Jiayan, Weng, Yiming, Wang, Jingsong, Wang, Tong, Song, Qibin, and Min, Peng
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- 2024
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11. Direct ink writing of multifunctional gratings with gel-like MXene/norepinephrine ink for dynamic electromagnetic interference shielding and patterned Joule heating
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Jiang, Pei-Zhu, Deng, Zhiming, Min, Peng, Ye, Lvxuan, Qi, Cheng-Zhang, Zhao, Hao-Yu, Liu, Ji, Zhang, Hao-Bin, and Yu, Zhong-Zhen
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- 2024
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12. A nationwide study on new onset atrial fibrillation risk factors and its association with hospital mortality in sepsis patients
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Yi-wei Liu, Yi-fan Wang, Yan Chen, Run Dong, Shan Li, Jin-min Peng, Rong Liufu, Li Weng, Yang Xu, and Bin Du
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Sepsis ,Atrial fibrillation ,Risk factor ,Cohort study ,Medicine ,Science - Abstract
Abstract Atrial fibrillation (AF) is the most common arrhythmia and its incidence increases with sepsis. However, data on new-onset AF during sepsis hospitalization remain limited in China. We aimed to evaluate the incidence, risk factors, and associated mortality of new-onset AF in sepsis patients in China. We conducted a retrospective study using the National Data Center for Medical Service system, from 1923 tertiary and 2363 secondary hospitals from 31 provinces in China from 2017 to 2019.In total we included 1,425,055 sepsis patients ≥ 18 years without prior AF. The incidence of new-onset AF was 1.49%. Older age, male sex, hypertension, heart failure, coronary disease, valvular disease, and mechanical ventilation were independent risk factor. New-onset AF was associated with a slight increased risk of mortality (adjusted RR 1.03, 95% CI 1.01–1.06). Population attributable fraction suggested AF accounted for 0.2% of sepsis deaths. In this large nationwide cohort, new-onset AF occurred in 1.49% of sepsis admissions and was associated with a small mortality increase. Further research should examine whether optimized AF management can improve sepsis outcomes in China.
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- 2024
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13. Genome-wide QTL and eQTL mapping reveal genes associated with growth rate trait of the Pacific white shrimp (Litopenaeus vannamei)
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Xiuli Chen, Min Peng, Chunling Yang, Qiangyong Li, Pengfei Feng, Weilin Zhu, Yongde Zhang, Digang Zeng, and Yongzhen Zhao
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Genetic map ,QTL ,eQTL ,Growth rate trait ,Litopenaeus vannamei ,Biotechnology ,TP248.13-248.65 ,Genetics ,QH426-470 - Abstract
Abstract Background Growth rate is a crucial economic trait for farmed animals, but the genetic regulation of this trait is largely unknown in non-model organisms such as shrimp. Results In this study, we performed genome-wide phenotypic quantitative trait loci (QTL) and expression quantitative trait loci (eQTL) mapping analyses to identify genes affecting the growth rate of Pacific white shrimp (Litopenaeus vannamei), which is the most commercially-farmed crustacean worldwide. We used RNA-sequencing of 268 individuals in a mapping population, and subsequently validated our findings through gene silencing and shrimp growth experiments. We constructed a high-density genetic linkage map comprising 5533 markers spanning 44 linkage groups, with a total distance of 6205.75 cM and an average marker interval of 1.12 cM. Our analyses identified 11 QTLs significantly correlated with growth rate, and 117,525 eQTLs. By integrating QTL and eQTL data, we identified a gene (metalloreductase STEAP4) highly associated with shrimp growth rate. RNA interference (RNAi) analysis and growth experiments confirmed that STEAP4 was significantly correlated with growth rate in L. vannamei. Conclusions Our results indicate that the comprehensive analysis of QTL and eQTL can effectively identify genes involved in complex animal traits. This is important for marker-assisted selection (MAS) of animals. Our work contributes to the development of shrimp breeding and available genetic resources.
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- 2024
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14. DialGNN: Heterogeneous Graph Neural Networks for Dialogue Classification
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Yan, Yan, Zhang, Bo-Wen, Min, Peng-hao, Ding, Guan-wen, and Liu, Jun-yuan
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- 2024
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15. A simple two-stage carrier-phase estimation algorithm for 32-QAM coherent optical communication systems
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Min Peng, Xiangqing Wang, Xiaokun Yang, and Dongfei Wang
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coherent optical communications ,quadrature amplitude modulation ,carrier phase estimation ,QPSK-partitioning ,blind phase search ,Physics ,QC1-999 - Abstract
The combination of high-order modulation formats and linewidth-tolerant carrier phase estimation (CPE) can effectively improve spectrum efficiency and relax the limitation of laser linewidth. This paper presents a simple two-stage CPE algorithm for polarization-multiplexed (PM) 32-quadrature amplitude modulation (32-QAM) coherent optical communication systems. The algorithm uses an enhanced QPSK partitioning algorithm combined with a simplified 4th power CPE method for coarse estimation in the initial stage and maximum likelihood (ML) detection in the subsequent fine stage. The CPE algorithm significantly increases the number of symbols used in the first stage of coarse estimation. This results in a significant increase in the stability and reliability of the phase estimation, and the CPE algorithm significantly reduces the computational complexity. The optimal parameters, phase estimation performance, and system performance of the algorithm were investigated by building a 22 Gbaud PM 32-QAM coherent system simulation platform and a 5 Gbaud PM 32-QAM coherent system experimental platform. The results show that the proposed two-stage CPE algorithm has a stronger linewidth tolerance difference than the conventional QPSK, and the two-stage CPE algorithm with an optimal block length of 105 performs comparable to blind phase search (BPS). The optical signal noise Ratio (OSNR) value is 21.2 dB and the bit error rate (BER) is 1.8 × 10−3 for the optimal block length of 105. The receiving-end DSP unit with a flexible scheme and good communication performance will have potential applications in adaptive elastic optical networks.
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- 2024
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16. Impact of ventilated tunnels on smoke peculiarities in train carriage fires with multiple lateral openings
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Zhenkun Wu, Min Peng, Yuelei Pan, Yifan Zhu, Wenxuan Miao, Ding Li, Dongzi Qin, Panwen Ma, and Guoqing Zhu
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Maximum ceiling excess temperature ,Longitudinal ventilation ,Subway ,Lateral openings ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
This research investigates the dynamics of smoke dispersion in a subway train carriage with multiple lateral openings within a ventilated subway tunnel. Numerous extensive full-scale numerical simulations were conducted to analyze the thermal plume distribution within multiple lateral openings of a train carriage, and the longitudinal and transverse maximum excess ceiling temperature. The results reveal that the heat release rates have a greater impact on the airflow in the carriage than velocities ,even when the longitudinal ventilation velocity reaches 8 m/s or the heat release rate reaches 15 MW. The multiple lateral openings cause significant differences in smoke flow characteristics compared to tunnels with both ends open. Additionally, the transverse maximum ceiling excess temperature inclines towards the lateral opening side due to asymmetrical air entrainment. The analysis of longitudinal maximum ceiling excess temperature identified two regions based on the dimensionless heat release rate, showing a linear increase followed by a constant phase dependent on the heat release rate, with a maximum longitudinal excess gas temperature of 1242 K. Additionally, a relationship between the dimensionless heat release rate and the transverse dimensionless maximum ceiling excess temperature was established. This study can be used as a guide for smoke exhaust mitigation and fire safety measures in the event of subway train carriage fires.
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- 2024
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17. CSformer: Enhancing deep learning efficiency for intelligent IoT.
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Xu Jia, Han Wu, Ruochen Zhang, and Min Peng
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- 2024
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18. A fault-tolerant scheduling algorithm that minimizes the number of replicas in heterogeneous service-oriented cloud computing systems.
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Fang Liu 0031, Kejie Hu, Jing He 0001, Wei Hu 0001, Heyuan Li, Min Peng 0002, and Yanxiang He
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- 2024
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19. Hierarchical Synergy-Enhanced Multimodal Relational Network for Video Question Answering.
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Min Peng 0004, Xiaohu Shao, Yu Shi 0003, and Xiangdong Zhou
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- 2024
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20. A Multiorientation Real-Time Respiration Monitoring System Using Wi-Fi Sensing Based on the Homofocal Hyperbola Model.
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Min Peng 0001, Haiyang Zhao, Xianxin Fu, and Yu Wang
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- 2024
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21. A Hardware Design Framework for Computer Vision Models Based on Reconfigurable Devices.
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Zimeng Fan 0001, Wei Hu 0001, Fang Liu 0031, Dian Xu, Hong Guo 0005, Yanxiang He, and Min Peng 0002
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- 2024
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22. Quantum Speed Limit under Brachistochrone Evolution
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Dou, Fu-Quan, Han, Min-Peng, and Shu, Chuan-Cun
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Quantum Physics - Abstract
According to the Heisenberg uncertainty principle between time and energy fluctuation, a concept of the quantum speed limit (QSL) has been established to determine the minimum evolutionary time between quantum states. Considerable theoretical and experimental efforts are invested in obtaining the QSL time bounds in various scenarios. However, it remains a long-standing goal to derive a meaningful QSL bound for a general quantum problem. Here, we propose a geometrical approach to derive a QSL bound for closed and open quantum systems. By solving a quantum brachistochrone problem in the framework of the Riemannian metric, we show that the QSL between a given initial state to a final state is determined not only by the entire dynamics of the system but also by the individual dynamics of a critical parameter. We exemplify the utility of the new bound in three representative scenarios, demonstrating a pronounced advantage in finding a tight and meaningful QSL bound of a general quantum evolution problem., Comment: 10 pages,4 figures
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- 2022
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23. Impact of Immunosuppressed Status on Prognosis of Carbapenem-Resistant Organisms Bloodstream Infections
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Yuan-Yuan Li, Yan Chen, Shan Li, Ran An, Xiao-Yun Hu, Wei Jiang, Chun-Yao Wang, Run Dong, Qi-Wen Yang, Li Weng, Jin-Min Peng, and Bin Du
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Carbapenem-resistant organisms (CROs) ,Immunosuppressed ,Bloodstream infection (BSI) ,Mortality ,Antimicrobial therapy ,Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract Introduction The impact of immunosuppression on prognosis of carbapenem-resistant organism (CRO) bloodstream infection (BSI) remains unclear. The aim of this study was to clarify the relationship between immunosuppression and mortality of CRO-BSI and to identify the risk factors associated with mortality in immunosuppressed patients. Methods This retrospective study included 279 patients with CRO-BSI from January 2018 to March 2023. Clinical characteristics and outcomes were compared between the immunosuppressed and immunocompetent patients. The relationship between immunosuppression and 30-day mortality after BSI onset was assessed through logistic-regression analysis, propensity score matching (PSM) and inverse probability of treatment weighting (IPTW). Factors associated with mortality in immunosuppressed patients were analyzed using multivariable logistic regression analysis. Results A total of 88 immunocompetent and 191 immunosuppressed patients were included, with 30-day all-cause mortality of 58.8%. Although the 30-day mortality in immunosuppressed patients was significantly higher than in immunocompetent patients (46.6% vs. 64.4%, P = 0.007), immunosuppression was not an independent risk factor for mortality in multivariate logistic regression analysis (odds ratio [OR] 3.53, 95% confidence interval [CI] 0.74–18.89; P = 0.123), PSM (OR 1.38, 95% CI 0.60–3.18; P = 0.449,) or IPTW (OR 1.40, 95% CI 0.58–3.36; P = 0.447). For patients with CRO-BSI, regardless of immune status, appropriate antibiotic therapy was associated with decreased 30-day mortality, while Charlson comorbidity index (CCI), intensive care unit (ICU)-acquired infection and thrombocytopenia at CRO-BSI onset were associated with increased mortality. Conclusion Despite the high mortality rate of CRO-BSI, immunosuppression did not affect the mortality. Appropriate antibiotic therapy is crucial for improving the prognosis of CRO-BSI, regardless of the immune status.
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- 2024
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24. FANCJ promotes PARP1 activity during DNA replication that is essential in BRCA1 deficient cells
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Ke Cong, Nathan MacGilvary, Silviana Lee, Shannon G. MacLeod, Jennifer Calvo, Min Peng, Arne Nedergaard Kousholt, Tovah A. Day, and Sharon B. Cantor
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Science - Abstract
Abstract The effectiveness of poly (ADP-ribose) polymerase inhibitors (PARPi) in creating single-stranded DNA gaps and inducing sensitivity requires the FANCJ DNA helicase. Yet, how FANCJ relates to PARP1 inhibition or trapping, which contribute to PARPi toxicity, remains unclear. Here, we find PARPi effectiveness hinges on S-phase PARP1 activity, which is reduced in FANCJ deficient cells as G-quadruplexes sequester PARP1 and MSH2. Additionally, loss of the FANCJ-MLH1 interaction diminishes PARP1 activity; however, depleting MSH2 reinstates PARPi sensitivity and gaps. Indicating sequestered and trapped PARP1 are distinct, FANCJ loss increases PARPi resistance in cells susceptible to PARP1 trapping. However, with BRCA1 deficiency, the loss of FANCJ mirrors PARP1 loss or inhibition, with the detrimental commonality being loss of S-phase PARP1 activity. These insights underline the crucial role of PARP1 activity during DNA replication in BRCA1 deficient cells and emphasize the importance of understanding drug mechanisms for enhancing therapeutic response.
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- 2024
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25. Routine evaluation of HBV-specific T cell reactivity in chronic hepatitis B using a broad-spectrum T-cell epitope peptide library and ELISpot assay
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Yandan Wu, Xiaotao Liu, Yuan Mao, Ruixue Ji, Lingzhi Xia, Zining Zhou, Yan Ding, Pinqing Li, Yu Zhao, Min Peng, Jie Qiu, and Chuanlai Shen
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Chronic hepatitis B ,Antigen-specific T cell ,T-cell epitopes ,ELISpot ,Medicine - Abstract
Abstract Background The clinical routine test of HBV-specific T cell reactivity is still limited due to the high polymorphisms of human leukocyte antigens (HLA) in patient cohort and the lack of universal detection kit, thus the clinical implication remains disputed. Methods A broad-spectrum peptide library, which consists of 103 functionally validated CD8+ T-cell epitopes spanning overall HBsAg, HBeAg, HBx and HBpol proteins and fits to the HLA polymorphisms of Chinese and Northeast Asian populations, was grouped into eight peptide pools and was used to establish an ELISpot assay for enumerating the reactive HBV-specific T cells in PBMCs. Totally 294 HBV-infected patients including 203 ones with chronic hepatitis B (CHB), 13 ones in acute resolved stage (R), 52 ones with liver cirrhosis (LC) and 26 ones with hepatocellular carcinoma (HCC) were detected, and 33 CHB patients were longitudinally monitored for 3 times with an interval of 3–5 months. Results The numbers of reactive HBV-specific T cells were significantly correlated with ALT level, HBsAg level, and disease stage (R, CHB, LC and HCC), and R patients displayed the strongest HBV-specific T cell reactivity while CHB patients showed the weakest one. For 203 CHB patients, the numbers of reactive HBV-specific T cells presented a significantly declined trend when the serum viral DNA load, HBsAg, HBeAg or ALT level gradually increased, but only a very low negative correlation coefficient was defined (r = − 0.21, − 0.21, − 0.27, − 0.079, respectively). Different Nucleotide Analogs (NUCs) did not bring difference on HBV-specific T cell reactivity in the same duration of treatment. NUCs/pegIFN-α combination led to much more reactive HBV-specific T cells than NUCs monotherapy. The dynamic numbers of reactive HBV-specific T cells were obviously increasing in most CHB patients undergoing routine treatment, and the longitudinal trend possess a high predictive power for the hepatitis progression 6 or 12 months later. Conclusion The presented method could be developed into an efficient reference method for the clinical evaluation of cellular immunity. The CHB patients presenting low reactivity of HBV-specific T cells have a worse prognosis for hepatitis progression and should be treated using pegIFN-α to improve host T-cell immunity.
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- 2024
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26. Cell-free DNA methylation reveals cell-specific tissue injury and correlates with disease severity and patient outcomes in COVID-19
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Yuan-Yuan Li, Ming-Ming Yuan, Shan Li, Jing-Dong Wang, Yu-Fei Wang, Qian Li, Jun Li, Rong-Rong Chen, Jin-Min Peng, and Bin Du
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Circulating-free DNA ,Coronavirus disease 2019 ,Immune response ,Tissue of origin inference ,Whole genome methylation sequencing ,Medicine ,Genetics ,QH426-470 - Abstract
Abstract Background The recently identified methylation patterns specific to cell type allows the tracing of cell death dynamics at the cellular level in health and diseases. This study used COVID-19 as a disease model to investigate the efficacy of cell-specific cell-free DNA (cfDNA) methylation markers in reflecting or predicting disease severity or outcome. Methods Whole genome methylation sequencing of cfDNA was performed for 20 healthy individuals, 20 cases with non-hospitalized COVID-19 and 12 cases with severe COVID-19 admitted to intensive care unit (ICU). Differentially methylated regions (DMRs) and gene ontology pathway enrichment analyses were performed to explore the locus-specific methylation difference between cohorts. The proportion of cfDNA derived from lung and immune cells to a given sample (i.e. tissue fraction) at cell-type resolution was estimated using a novel algorithm, which reflects lung injuries and immune response in COVID-19 patients and was further used to evaluate clinical severity and patient outcome. Results COVID‑19 patients had globally reduced cfDNA methylation level compared with healthy controls. Compared with non-hospitalized COVID-19 patients, the cfDNA methylation pattern was significantly altered in severe patients with the identification of 11,156 DMRs, which were mainly enriched in pathways related to immune response. Markedly elevated levels of cfDNA derived from lung and more specifically alveolar epithelial cells, bronchial epithelial cells, and lung endothelial cells were observed in COVID-19 patients compared with healthy controls. Compared with non-hospitalized patients or healthy controls, severe COVID-19 had significantly higher cfDNA derived from B cells, T cells and granulocytes and lower cfDNA from natural killer cells. Moreover, cfDNA derived from alveolar epithelial cells had the optimal performance to differentiate COVID-19 with different severities, lung injury levels, SOFA scores and in-hospital deaths, with the area under the receiver operating characteristic curve of 0.958, 0.941, 0.919 and 0.955, respectively. Conclusion Severe COVID-19 has a distinct cfDNA methylation signature compared with non-hospitalized COVID-19 and healthy controls. Cell type-specific cfDNA methylation signature enables the tracing of COVID-19 related cell deaths in lung and immune cells at cell-type resolution, which is correlated with clinical severities and outcomes, and has extensive application prospects to evaluate tissue injuries in diseases with multi-organ dysfunction.
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- 2024
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27. Neutral ceramidase regulates breast cancer progression by metabolic programming of TREM2-associated macrophages
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Rui Sun, Chao Lei, Zhishan Xu, Xuemei Gu, Liu Huang, Liang Chen, Yi Tan, Min Peng, Kavitha Yaddanapudi, Leah Siskind, Maiying Kong, Robert Mitchell, Jun Yan, and Zhongbin Deng
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Science - Abstract
Abstract The tumor microenvironment is reprogrammed by cancer cells and participates in all stages of tumor progression. Neutral ceramidase is a key regulator of ceramide, the central intermediate in sphingolipid metabolism. The contribution of neutral ceramidase to the reprogramming of the tumor microenvironment is not well understood. Here, we find that deletion of neutral ceramidase in multiple breast cancer models in female mice accelerates tumor growth. Our result show that Ly6C+CD39+ tumor-infiltrating CD8 T cells are enriched in the tumor microenvironment and display an exhausted phenotype. Deletion of myeloid neutral ceramidase in vivo and in vitro induces exhaustion in tumor-infiltrating Ly6C+CD39+CD8+ T cells. Mechanistically, myeloid neutral ceramidase is required for the generation of lipid droplets and for the induction of lipolysis, which generate fatty acids for fatty-acid oxidation and orchestrate macrophage metabolism. Metabolite ceramide leads to reprogramming of macrophages toward immune suppressive TREM2+ tumor associated macrophages, which promote CD8 T cells exhaustion.
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- 2024
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28. Rechallenge of immune checkpoint inhibitors in advanced non‐small cell lung cancer
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Gen Lin, Zhijie Wang, Qian Chu, Yi Hu, Dingzhi Huang, Jun Wang, Fan Yang, Wenzhao Zhong, Chengzhi Zhou, Bo Zhu, Xinghao Ai, Baoshan Cao, Yabing Cao, Mingqiu Chen, Xiaohui Chen, Tianqing Chu, Jianchun Duan, Yun Fan, Yong Fang, Shuitu Feng, Weineng Feng, Hui Guo, Chengbo Han, Yong He, Shaodong Hong, Jie Hu, Meijuan Huang, Yan Huang, Da Jiang, Kan Jiang, Richeng Jiang, Bo Jin, Shi Jin, Jisheng Li, Min Li, Ziming Li, Chao Li, Jie Lin, Anwen Liu, Si‐Yang Maggie Liu, Liu Yutao, Zhefeng Liu, Zhe Liu, Zhenhua Liu, Zhentian Liu, Zhigang Liu, Yuping Lu, Tangfeng Lv, Zhiyong Ma, Qian Miao, Min Peng, Xingxiang Pu, Xiu Bao Ren, Jianzhen Shan, Jinlu Shan, Peng Shen, Bo Shen, Meiqi Shi, Yong Song, Zhengbo Song, ChunXia Su, Jianguo Sun, Panwen Tian, Jinliang Wang, Feng Wang, Huijuan Wang, Jialei Wang, Qian Wang, Wenxian Wang, Yan Wang, Lin Wu, Fang Wu, Yang Xia, Congying Xie, Conghua Xie, Tao Xin, Jianping Xiong, Haipeng Xu, Song Xu, Yiquan Xu, Bin Xu, Chunwei Xu, Xiaolong Yan, Zhenzhou Yang, Wenxiu Yao, Yao Yu, Ye Feng, Zongyang Yu, Yongfeng Yu, Dongsheng Yue, Haibo Zhang, HongMei Zhang, Li Zhang, Longfeng Zhang, Qiuyu Zhang, Tongmei Zhang, Bicheng Zhang, Jun Zhao, Mingfang Zhao, Xiaobin Zheng, Qiaofeng Zhong, Jin Zhou, Penghui Zhou, Zhengfei Zhu, Juntao Zou, and Zihua Zou
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ICI ,NSCLC ,re‐challenge ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Immune checkpoint inhibitor (ICI) rechallenge in non‐small cell lung cancer (NSCLC) is a promising therapeutic strategy. The situation for ICI rechallenge can be divided into three categories: adverse events (AEs); resistance to ICIs, and rechallenge becomes compulsive because of tumor relapse while the patients had completed a 2 year course of immunotherapy. However, these categories are still controversial and should be explored further. Through voting at the 6th Straits Summit Forum on Lung Cancer, in this study we summarize the consensus of 147 experts in ICI rechallenges. A total of 97.74% experts agreed to rechallenge; 48.87% experts rechallenge with the original drug, and the others rechallenge with a different drug; 40.3% agreed to rechallenge directly after progression; 88.06% experts agreed to ICI rechallenge with a combination regimen; and factors such as previous performance status score, PD‐1 expression, and age should also be considered. Understanding the the clinical studies in ICI rechallenge could bring us one step closer to understanding the consensus. In patients with advanced NSCLC who have suffered recurrent or distant metastasis after immunotherapy, the option of rechallenge with ICIs is a promising treatment option.
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- 2024
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29. The first case of intellectual disability caused by novel compound heterozygosity for NUDT2 variants
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Bo Bi, Xiaohong Chen, Shan Huang, Min Peng, Weiyue Gu, Hongmin Zhu, and Yangcan Ming
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Developmental delay ,Intellectual disability ,Motor delay ,NUDT2 ,Pediatrics ,RJ1-570 - Abstract
Abstract NUDT2 is an enzyme important for maintaining the intracellular level of the diadenosine tetraphosphate (Ap4A). Bi-allelic loss of function variants in NUDT2 has recently been reported as a rare cause of intellectual disability (ID). Herein, we describe a Chinese girl with ID, attention deficit hyperactivity disorder (ADHD), and motor delays with abnormal walking posture and difficulty climbing stairs, who bears compound heterozygous variants c.34 C > T (p.R12*) and c.194T > G (p.I65R) in NUDT2. Homozygous variants c.34 C > T (p.R12*) or c.186del (p.A63Qfs*3) in NUDT2 were previously reported to cause ID. This is the first patient with ID due to compound heterozygous variants in NUDT2 and p.I65R is a novel missense variant. This study enriched the genotype and phenotype of NUDT2-related ID and supported the critical developmental involvement of NUDT2.
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- 2024
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30. E2F transcription factor 5, a new regulator in adipogenesis to mediate the role of Krüppel-like factor 7 in chicken preadipocyte differentiation and proliferation
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Ziqiu Jia, Zhao Jin, Meiqi Li, Xin Zhang, Min Peng, Shanshan Zhang, Ming Tan, Qingzhu Yang, Weiyu Wang, and Yingning Sun
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chicken ,E2F5 ,KLF7 ,preadipocyte ,Animal culture ,SF1-1100 - Abstract
ABSTRACT: E2F transcription factor 5 (E2F5) gene is a transcription factor, plays an important role in the development of a variety of cells. E2F5 is expressed in human and mouse adipocytes, but its specific function in adipogenesis is unclear. Krüppel-like factor 7 (KLF7) facilitates proliferation and inhibits differentiation in chicken preadipocytes. Our previous KLF7 chromatin immunoprecipitation-sequencing analysis revealed a KLF7-binding peak in the 3′ flanking region of the E2F5, indicating a regulatory role of KLF7 in this region. In the present study, we investigated E2F5 potential role, the overexpression and knockdown analyses revealed that E2F5 inhibited the differentiation and promoted the proliferation of chicken preadipocytes. Moreover, we identified enhancer activity in the 3′ flanking region (nucleotides +22661/+22900) of E2F5 and found that KLF7 overexpression increased E2F5 expression and luciferase activity in this region. Deleting the putative KLF7-binding site eliminated the promoting effect of KLF7 overexpression on E2F5 expression. Further, E2F5 reversed the KLF7-induced decrease in preadipocyte differentiation and increase in preadipocyte proliferation. Taken together, our findings demonstrate that KLF7 inhibits differentiation and promotes proliferation in preadipocytes by enhancing E2F5 transcription.
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- 2024
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31. Knowledge, attitudes, and practices among patients with anemia towards disease management
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Binlian Yao, Min Xu, Fang Cheng, Min Peng, and Xiaopei Mao
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knowledge ,attitudes ,practices ,anemia ,cross-sectional study ,Public aspects of medicine ,RA1-1270 - Abstract
ObjectiveThis study aimed to assess the knowledge, attitudes and practices among anemia patients toward disease management.MethodsThis web-based cross-sectional study was conducted between September and December 2023 at The First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine). A self-designed questionnaire was developed to collect demographic information of anemia patients, and assess their knowledge, attitudes and practices (KAP) toward disease management.ResultsA total of 396 valid questionnaires were collected. The mean age of the participants was 57.44 ± 16.80 years, and 52.02% were female. The mean knowledge, attitudes, and practices scores were 11.47 ± 1.73 (possible range: 0–14), 27.32 ± 2.96 (possible range: 7–35), and 40.49 ± 6.06 (possible range: 10–50), respectively. Multivariate analysis showed that bachelor’s degree or above was independently associated with sufficient knowledge (OR = 2.372, 95%CI: 1.160–4.853, p = 0.018). Knowledge (OR = 1.350, 95%CI: 1.166–1.563, p
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- 2024
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32. Evaluation of system based psychological first aid training on the mental health proficiency of emergency medical first responders to natural disasters in China: a cluster randomised controlled trial
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Ben Carter, Tao Xiao, James Shearer, and Min Peng
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Medicine - Abstract
Objective To evaluate the effectiveness of a system based psychological first aid (PFA) training programme for emergency medical first responders in China.Design Parallel-group, assessor-blinded, cluster randomised controlled trial.Setting 42 clusters of health workers from various health facilities in China.Participants 1399 health workers who provide emergency service for survivors of disasters.Interventions One-day system based PFA training programme (PFA) or training as usual (TAU).Primary and secondary outcome measures The primary outcome was the PFA skills, knowledge and attitude (SKA-PFA) score at 2 months postintervention. Secondary outcomes included post-traumatic growth, self-efficacy and professional quality of life.Results The intervention group (n=690) had significantly higher SKA-PFA scores than the control group (n=709) at 2 months postintervention (adjusted mean difference=4.44; 95% CI 1.17 to 7.52; p=0.007; Cohen’s d=0.35). The intervention group also had higher scores on post-traumatic growth (p=0.113, d=0.24), self-efficacy (p=0.032, d=0.20) and professional quality of life (p=0.281, d=0.04).Conclusions The system based PFA training programme was more effective than the TAU in enhancing the PFA knowledge and skills of the emergency medical first responders and in increasing their competence to provide emergency service for survivors in China.Trial registration number ChiCTR2200060464.
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- 2024
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33. Acupuncture for generalized anxiety disorder: a study protocol for a randomized controlled trial
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Xiayun Zhou, Guoao Shi, Ruiming Chen, Lingsan Hu, Zhongxian Li, Yifu Zhou, Pan Zhang, Xiang Ji, Min Peng, Kengyu Chen, Luda Yan, and Peng Zhou
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acupuncture ,generalized anxiety disorder ,cerebral cortical excitability ,transcranial magnetic stimulation ,randomized controlled trial ,study protocol ,Medicine (General) ,R5-920 ,Biology (General) ,QH301-705.5 - Abstract
During the COVID-19 outbreak, there was a sharp increase in generalized anxiety disorder (GAD). Acupuncture therapy has the advantages of accurate clinical efficacy, safety and reliability, few adverse reactions, and no dependence, and is gradually becoming one of the emerging therapies for treating GAD. We present a study protocol for a randomized clinical trial with the aim of exploring the mechanism of brain plasticity in patients with GAD and evaluate the effectiveness and reliability of acupuncture treatment. Transcranial magnetic stimulation (TMS) will be used to assess cortical excitability in GAD patients and healthy people. Sixty-six GAD patients meeting the inclusion criteria will be randomly divided into two groups: TA group, (treatment with acupuncture and basic western medicine treatment) and SA group (sham acupuncture and basic western medicine treatment). Twenty healthy people will be recruited as the control group (HC). The parameters that will be evaluated are amplitude of motor evoked potentials (MEPs), cortical resting period (CSP), resting motor threshold (RMT), and Hamilton Anxiety Scale (HAMA) score. Secondary results will include blood analysis of γ-aminobutyric acid (GABA), glutamate (Glu), glutamine (Gln), serotonin (5-HT), and brain-derived nerve growth factor (BDNF). Outcomes will be assessed at baseline and after the intervention (week 8). This study protocol is the first clinical trial designed to detect differences in cerebral cortical excitability between healthy subjects and patients with GAD, and the comparison of clinical efficacy and reliability before and after acupuncture intervention is also one of the main contents of the protocol. We hope to find a suitable non-pharmacological alternative treatment for patients with GAD.
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- 2024
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34. Effects of Selenium and Cadmium on Human Liver and Kidney Functions in Exposed Black Shale Areas
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Minglong Li, Liang Qiu, Xunru Ai, Keyuan Xu, Min Peng, Guogen Sun, Kai Zhang, and Chuying Huang
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selenium and cadmium ,antagonism ,liver and kidney functions ,black shale‐exposed area ,USEPA method ,enshi ,Environmental protection ,TD169-171.8 - Abstract
Abstract Animal experiments suggest that selenium (Se) may alleviate cadmium (Cd) toxicity in animal liver and kidneys, but its effect on human liver and kidneys remains uncertain. In China, areas with black shale have shown elevated levels of Se and Cd. According to the USEPA (U.S. Environmental Protection Agency) evaluation method, the soil and rice in these areas pose significant risks. In black shale regions such as Enshi and Zhuxi County, residents who long‐term consume local rice may surpass safe Se and Cd intake levels. Significantly high median blood Se (B‐Se) and urine selenium (U‐Se) levels were detected in these areas, measuring 416.977 μg/L and 352.690 μg/L and 104.527 μg/L and 51.820 μg/L, respectively. Additionally, the median blood Cd (B‐Cd) and urine Cd (U‐Cd) levels were markedly elevated at 4.821 μg/L and 3.848 μg/L and at 7.750 μg/L and 7.050 μg/L, respectively, indicating substantial Cd exposure. Nevertheless, sensitive liver and kidney biomarkers in these groups fall within healthy reference ranges, suggesting a potential antagonistic effect of Se on Cd in the human body. Therefore, the USEPA method may not accurately assess Cd risk in exposed black shale areas. However, within the healthy ranges, residents in the Enshi study area had significantly greater median levels of serum creatinine and cystatin C, measuring 67.3 μmol/L and 0.92 mg/L, respectively, than those in Zhuxi did (53.6 μmol/L and 0.86 mg/L). In cases of excessive Se and Cd exposure, high Se and Cd levels impact the filtration function of the human kidney to some extent.
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- 2024
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35. Case report: A germline CHEK1 c.613 + 2T>C leads to a splicing error in a family with multiple cancer patients
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Jun Qian, Min Peng, Yanan Li, Wei Liu, Xinwei Zou, Huafei Chen, Sujuan Zhou, Sheng Xiao, and Jinhua Zhou
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CHEK1 ,germline mutation ,splicing error ,ovarian cancer ,inherited cancer ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
BackgroundGenome instability plays a crucial role in promoting tumor development. Germline mutations in genes responsible for DNA repair are often associated with familial cancer syndromes. A noticeable exception is the CHEK1 gene. Despite its well-established role in homologous recombination, germline mutations in CHEK1 are rarely reported.Case presentationIn this report, we present a patient diagnosed with ovarian clear cell carcinoma who has a family history of cancer. Her relatives include a grandfather with esophageal cancer, a father with gastric cancer, and an uncle with a brain tumor. The patient carried a typical genomic profile of clear cell carcinoma including mutations in KRAS, PPP2R1A, and PIK3R1. Importantly, her paired peripheral blood cells harbored a germline CHEK1 mutation, CHEK1 exon 6 c.613 + 2T>C, which was also found in her father. Unfortunately, the CHEK1 status of her grandfather and uncle remains unknown due to the unavailability of their specimens. Further evaluation via RT-PCR confirmed a splicing error in the CHEK1 gene, resulting in truncation at the kinase domain region, indicative of a loss-of-function mutation.ConclusionThis case highlights a rare germline CHEK1 mutation within a family with a history of cancer. The confirmed splicing error at the mRNA level underscores the functional consequences of this mutation. Documenting such cases is vital for future evaluation of inheritance patterns, clinical penetrance of the mutation, and its association with specific cancer types.
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- 2024
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36. Study on the influence of restricted distance on fixed-width discrete of discontinuous flame spread
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Zhenkun Wu, Min Peng, Jinhu Zhou, Panwen Ma, Dongzi Qin, and Guoqing Zhu
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Flame spread ,Confined spaces ,PMMA ,Discrete solid ,Engineering (General). Civil engineering (General) ,TA1-2040 - Abstract
This study investigates the impact of varying distances between vertical walls on the propagation of discrete flames in PMMA (Polymethyl Methacrylate) slabs. The primary objective is to analyze the dynamics of flame spread in confined spaces, with a particular focus on how the spacing between vertical walls and the number of PMMA slabs influence flame characteristics. Experimentally, flame spread rate, mass loss rate, heat release rate, and pyrolysis front dynamics are examined under different confined space distances ranging from 1 to 5 cm using thermally thick PMMA slabs. The findings reveal a significant influence of confined space distance on flame propagation. In narrower spaces (1 to 2 cm), a stack effect leads to a rapid increase in flame height and mass loss rate. As the confined distance increases, this effect gradually diminishes, resulting in a decrease in flame height and mass loss rate. Additionally, the flame spread rate initially rises with increasing confined distance, reaching a peak at 2 cm, before decreasing. Through the application of the law of conservation of energy, the dominant mechanisms of flame spread are deeply analyzed under various confined space conditions. These insights are crucial for understanding and predicting fire spread in confined spaces.
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- 2024
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37. Controllable multiple beam splitting in Hermitian and non-Hermitian symmetric coupled waveguide systems
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Dou, Fu-Quan, Wei, Ya-Ting, Han, Min-Peng, and Sun, Jian-An
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Physics - Optics ,Quantum Physics - Abstract
We investigate high-fidelity multiple beam splitting in Hermitian and non-Hermitian symmetric coupled waveguides with one input and 2N output waveguide channels. In Hermitian systems, we realize adiabatically light splitting in resonant case based on the stimulated Raman adiabatic passage (STIRAP) and arbitrary proportion from the middle waveguide to outer waveguides in propagation coefficients mismatch case using shortcuts to adiabaticity (STA) technique. In non-Hermitian systems with even waveguides being dissipative, the compact and robust beam splitting can be achieved by eliminating the non-adiabatic coupling via the non-Hermitian STA method. We further verify the feasibility of our theoretical predictions by means of the beam propagation method (BPM). The suggested multiple beam splitters open new opportunities for the realization of on-chip high-bandwidth photonics with high fidelity in short distances., Comment: Accepted
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- 2022
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38. Clinical definition of secondary resistance to immunotherapy in non‐small cell lung cancer
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Dingzhi Huang, Gen Lin, Qian Chu, Yi Hu, Jun Wang, Zhijie Wang, Fan Yang, Wenzhao Zhong, Chengzhi Zhou, Bo Zhu, Xinghao Ai, Baoshan Cao, Yabing Cao, Mingqiu Chen, Xiaohui Chen, Tianqing Chu, Jianchun Duan, Yun Fan, Yong Fang, Shuitu Feng, Weineng Feng, Hui Guo, Chengbo Han, Yong He, Shaodong Hong, Jie Hu, Meijuan Huang, Yan Huang, Da Jiang, Kan Jiang, Richeng Jiang, Bo Jin, Shi Jin, Jisheng Li, Min Li, Ziming Li, Chao Li, Jie Lin, Anwen Liu, Si‐Yang Maggie Liu, Yutao Liu, Zhefeng Liu, Zhe Liu, Zhenhua Liu, Zhentian Liu, Zhigang Liu, Yuping Lu, Tangfeng Lv, Zhiyong Ma, Qian Miao, Min Peng, Xingxiang Pu, Xiu Bao Ren, Jianzhen Shan, Jinlu Shan, Peng Shen, Bo Shen, Meiqi Shi, Yong Song, Zhengbo Song, ChunXia Su, Jianguo Sun, Panwen Tian, Jinliang Wang, Feng Wang, Huijuan Wang, Jialei Wang, Qian Wang, Wenxian Wang, Yan Wang, Lin Wu, Fang Wu, Yang Xia, Congying Xie, Conghua Xie, Tao Xin, Jianping Xiong, Haipeng Xu, Song Xu, Yiquan Xu, Bin Xu, Chunwei Xu, Xiaolong Yan, Zhenzhou Yang, Wenxiu Yao, Yao Yu, Ye Feng, Zongyang Yu, Yongfeng Yu, Dongsheng Yue, Haibo Zhang, HongMei Zhang, Li Zhang, Longfeng Zhang, Qiuyu Zhang, Tongmei Zhang, Bicheng Zhang, Jun Zhao, Mingfang Zhao, Xiaobin Zheng, Fengqiao Zhong, Jin Zhou, Penghui Zhou, Zhengfei Zhu, Juntao Zou, and Zihua Zou
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immunotherapy ,NSCLC ,secondary resistance ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Immune checkpoint inhibitors (PD‐1/PD‐L1 and CTLA‐4 blockade) have revolutionized the treatment landscape in non‐small cell lung cancer (NSCLC). Secondary resistance to immunotherapy (IO), which poses a substantial challenge in clinical settings, occurs in several initial responders. Currently, new treatment approaches have been extensively evaluated in investigational studies for these patients to tackle this difficult problem; however, the lack of consistency in clinical definition, uniform criteria for enrollment in clinical trials, and interpretation of results remain significant hurdles to progress. Thus, our expert panel comprehensively synthesized data from current studies to propose a practical clinical definition of secondary resistance to immunotherapy in NSCLC in metastatic and neoadjuvant settings. In addition to patients who received IO alone (including IO‐IO combinations), we also generated a definition for patients treated with chemotherapy plus IO. This consensus aimed to provide guidance for clinical trial design and facilitate future discussions with investigators. It should be noted that additional updates in this consensus are required when new data is available.
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- 2023
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39. Computed tomography imaging and clinical characteristics of pulmonary ground‐glass nodules ≤2 cm with micropapillary pattern
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Chen‐ran Guo, Lin Xu, Xiao Li, Yi‐lin Fu, Hui Wang, Rui Han, Geng‐sheng Li, Zhen Feng, Meng Li, Wan‐gang Ren, and Zhong‐min Peng
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ground‐glass nodules ,lung adenocarcinoma ,lymph nodes ,micropapillary pattern ,morphological ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Background The aim of this study was to investigate the imaging features, lymph node metastasis, and genetic mutations in micropapillary lung adenocarcinoma (imaging with mixed ground‐glass nodules) ≤2 cm, to provide a more precise and refined basis for the selection of lung segment resection. Methods A retrospective analysis of 162 patients with surgically resected pathologically confirmed cancers ≤2.0 cm in diameter (50 cases of micropapillary mixed ground‐glass nodules [mGGNs], 50 cases of nonmicropapillary mGGNs, and 62 cases of micropapillary SNs [solid nodules]) was performed. mGGNs were classified into five categories according to imaging features. The distribution of these five morphologies in micropapillary with mGGN and nonmicropapillary with mGGN was analyzed. The postoperative pathology and prognosis of lymph node metastasis were also compared between micropapillary mGGNs and micropapillary with SNs. After searching the TCGA database, we demonstrated heterogeneity, high malignancy and high risk of microcapillary lung cancer cancers. Results Different pathological subtypes of mGGN differed in morphological features (p
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- 2023
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40. Construction of silver-coated high translucent zirconia implanting abutment material and its property of antibacterial
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Min Peng, Jun-Lan Chuan, Gao-ping Zhao, and Qiang Fu
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Nano-Ag ,aluminosilicate ,implanting abutment ,high translucent zirconia ,antibacterial property ,Biotechnology ,TP248.13-248.65 ,Medical technology ,R855-855.5 - Abstract
AbstractHigh translucent zirconia (HTZ) has excellent mechanical properties, biocompatibility, and good semi-translucency making it an ideal material for aesthetic anterior dental implant abutments without antibacterial properties. In the oral environment, the surface of the abutment material is susceptible to microbial adhesion and biofilm formation, which can lead to infection or peri-implantitis and even implant failure. This study aims to promote the formation of a biological seal at the implant-soft tissue interface by modifying the HTZ surface, using the load-bearing capacity of the aluminosilicate porous structure and the broad-spectrum antibacterial effect of silver nanoparticles to prevent peri-implant bacterial infection and inflammation and to improve the success rate and prolong the use of the implant. FE-SEM (field emission scanning electron microscopes), EDS (energy dispersive spectroscopy), and XPS (X-ray photoelectron spectroscopy) results showed that aluminosilicate non-vacuum sintering can form open micro- and nanoporous structures on HTZ surfaces, and that porous aluminosilicate coatings obtain a larger number, smaller size, and more uniformly shaped silver nanoparticles than smooth aluminosilicate coatings, and could be deposited deeper in the coating. The ICP-AES (inductively coupled plasma-atomic emission spectroscopy) results showed that the early silver ion release of both the smooth silver coating and the porous silver coating was obvious, the silver ion concentration released by the former was higher than that of the latter. However, the silver ion concentration released by the porous silver coating was higher than that of the smooth coating when the release slowed down. Both smooth and porous silver coatings both inhibited E. coli (Escherichia coli), S. aureus (Staphylococcus aureus), and L. acidophilus (L. acidophilus), and porous silver coatings had stronger antibacterial properties. The silver coating was successfully constructed on the surface of HTZ, through aluminium silicate sintering and silver nitrate solution impregnation. It was found that the high concentration environment of silver nitrate solution was more advantageous for nano-Ag deposition, and the non-vacuum sintered porous surface was able to obtain a larger number of nano-Ag particles with smaller sizes. The porous Ag coating exhibited superior antibacterial properties. It was suggested that the HTZ with silver coating had clinical application, and good antibacterial properties that can improve the survival rate and service life of implants.
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- 2023
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41. Economic Research on Energy Storage Auxiliary Frequency Regulation of Lithium Iron Phosphate Battery for 2 × 600 MW Coal-fired Unit in Guangdong
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Fan WANG, Binsi LI, Tongling XIA, Min PENG, and Shaoyong WANG
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energy storage ,coal-fired power plant ,combined frequency regulation ,economy ,lithium iron phosphate battery ,Energy industries. Energy policy. Fuel trade ,HD9502-9502.5 - Abstract
[Introduction] In view of the economic benefits of AGC frequency regulation project of combined energy storage in Guangdong coal-fired power plant, the method of establishing typical engineering cases is demonstrated. [Method] This article summarized the latest version of frequency regulation auxiliary market revenue settlement rules in the southern region and calculated the frequency regulation performance index of typical 2 × 600 MW coal-fired units using lithium iron phosphate battery energy storage in Guangdong Province, then established a revenue model, estimated or assumed the key parameters such as cost, mileage, clearing price, running time, etc. Finally, under the contract energy management mode, it calculated its economy from the perspective of investors and analyzed the changes of financial index under different total investment, operation year and revenue sharing. [Result] The results show that in the measured case, except for the first sharing scheme, the internal rate of return of capital in other scenarios all exceeds 7%. [Conclusion] The frequency regulation project of lithium iron phosphate battery energy storage in Guangdong has a good return on investment within four years. After that, investors can still be attracted to participate in this project with the decrease of total investment and the increase of share.
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- 2023
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42. FUS-mediated HypEVs: Neuroprotective effects against ischemic stroke
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Yousheng Wu, Xiaoxiong Huang, Zefeng Tan, Jiankun Zang, Min Peng, Niu He, Tao Zhang, Hongcheng Mai, Anding Xu, and Dan Lu
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Hypoxia ,FUS ,Neuron-derived small extracellular vesicle ,Neuroprotection ,Mitochondrial mRNA ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biology (General) ,QH301-705.5 - Abstract
Few studies have investigated the properties and protein composition of small extracellular vesicles (sEVs) derived from neurons under hypoxic conditions. Presently, the extent of the involvement of these plentiful sEVs in the onset and progression of ischemic stroke remains an unresolved question. Our study systematically identified the characteristics of sEVs derived from neurons under hypoxic conditions (HypEVs) by physical characterization, sEV absorption, proteomics and transcriptomics analysis. The effects of HypEVs on neurites, cell survival, and neuron structure were assessed in vitro and in vivo by neural complexity tests, magnetic resonance imaging (MRI), Golgi staining, and Western blotting of synaptic plasticity-related proteins and apoptotic proteins. Knockdown of Fused in Sarcoma (FUS) small interfering RNA (siRNA) was used to validate FUS-mediated HypEV neuroprotection and mitochondrial mRNA release. Hypoxia promoted the secretion of sEVs, and HypEVs were more easily taken up and utilized by recipient cells. The MRI results illustrated that the cerebral infarction volume was reduced by 45% with the application of HypEVs, in comparison to the non- HypEV treatment group. Mechanistically, the FUS protein is necessary for the uptake and neuroprotection of HypEVs against ischemic stroke as well as carrying a large amount of mitochondrial mRNA in HypEVs. However, FUS knockdown attenuated the neuroprotective rescue capabilities of HypEVs. Our comprehensive dataset clearly illustrates that FUS-mediated HypEVs deliver exceptional neuroprotective effects against ischemic stroke, primarily through the maintenance of neurite integrity and the reduction of mitochondria-associated apoptosis.
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- 2023
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43. ZSCAN4 interacts with PARP1 to promote DNA repair in mouse embryonic stem cells
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Li-Kuang Tsai, Min Peng, Chia-Chun Chang, Luan Wen, Lin Liu, Xiubin Liang, Y. Eugene Chen, Jie Xu, and Li-Ying Sung
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ZSCAN4 ,PARP1 ,DNA double strand breaks ,Mouse embryonic stem cells ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 ,Biochemistry ,QD415-436 - Abstract
Abstract Background In eukaryotic cells, DNA double strand breaks (DSB) are primarily repaired by canonical non-homologous end joining (c-NHEJ), homologous recombination (HR) and alternative NHEJ (alt-NHEJ). Zinc finger and SCAN domain containing 4 (ZSCAN4), sporadically expressed in 1–5% mouse embryonic stem cells (mESCs), is known to regulate genome stability by promoting HR. Results Here we show that ZSCAN4 promotes DNA repair by acting with Poly (ADP-ribose) polymerase 1 (PARP1), which is a key member of the alt-NHEJ pathway. In the presence of PARP1, ZSCAN4-expressing mESCs are associated with lower extent of endogenous or chemical induced DSB comparing to ZSCAN4-negative ones. Reduced DSBs associated with ZSCAN4 are abolished by PARP1 inhibition, achieved either through small molecule inhibitor or gene knockout in mESCs. Furthermore, PARP1 binds directly to ZSCAN4, and the second ⍺-helix and the fourth zinc finger motif of ZSCAN4 are critical for this binding. Conclusions These data reveal that PARP1 and ZSCAN4 have a protein–protein interaction, and shed light on the molecular mechanisms by which ZSCAN4 reduces DSB in mESCs.
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- 2023
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44. Tracking tumor alteration in glioma through serum fibroblast activation protein combined with image
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Xiao-song Yang, Peng zhu, Rong-Xing Xie, Peng-fei Chen, Hong Liu, Xiao-Man Cheng, Zheng-Quan Zhu, Xiao-min Peng, Hai-bin Liu, Qun-Ying Yang, Jun-Qi Li, and Ji Zhang
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Glioma ,Fibroblast activation protein ,Image ,Serum ,Dynamic detection ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Abstract Purpose Detecting tumor progression of glioma continues to pose a formidable challenge. The role of fibroblast activation protein (FAP) in gliomas has been demonstrated to facilitate tumor progression. Glioma-circulating biomarkers have not yet been used in clinical practice. This study seeks to evaluate the feasibility of glioma detection through the utilization of a serum FAP marker. Methods We adopted enzyme-linked immunosorbent assay (ELISA) technique to quantify the relative FAP level of serum autoantibodies in a cohort of 87 gliomas. The correlation between preoperative serum autoantibody relative FAP levels and postoperative pathology, including molecular pathology was investigated. A series of FAP tests were conducted on 33 cases of malignant gliomas in order to ascertain their efficacy in monitoring the progression of the disease in relation to imaging observations. To validate the presence of FAP expression in tumors, immunohistochemistry was conducted on four gliomas employing a FAP-specific antibody. Additionally, the investigation encompassed the correlation between postoperative tumor burden, as assessed through volumetric analysis, and the relative FAP level of serum autoantibodies. Results A considerable proportion of gliomas exhibited a significantly increased level of serum autoantibody relative FAP level. This elevation was closely associated with both histopathology and molecular pathology, and demonstrated longitudinal fluctuations and variations corresponding to the progression of the disease The correlation between the rise in serum autoantibody relative FAP level and tumor progression and/or exacerbation of symptoms was observed. Conclusions The measurement of serum autoantibody relative FAP level can be used to detect the disease as a valuable biomarker. The combined utilization of its detection alongside MR imaging has the potential to facilitate a more accurate and prompt diagnosis.
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- 2023
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45. Causality between heart failure and epigenetic age: a bidirectional Mendelian randomization study
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Fengjun Zhang, Shanshan Deng, Jing Zhang, Wenchang Xu, Dexian Xian, Yuxuan Wang, Qiong Zhao, Yuan Liu, Xiuli Zhu, Min Peng, and Lin Zhang
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Epigenetic age ,Heart failure (HF) ,Causality ,Bidirectional Mendelian randomization (MR) study ,Genome‐wide association study (GWAS) ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Abstract Aims Heart failure (HF) is a prevalent age‐related cardiovascular disease with poor prognosis in the elderly population. This study aimed to establish the causal relationship between ageing and HF by conducting a bidirectional Mendelian randomization (MR) analysis on epigenetic age (a marker of ageing) and HF. Methods and results Genome‐wide association study data for epigenetic age (GrimAge, HorvathAge, HannumAge, and PhenoAge) and HF were collected and assessed for significant genetic variables. A bidirectional MR analysis was carried out using the random‐effects inverse–variance weighted (IVW) method as the primary approach, while other methods (MR–Egger, weighted median, simple mode, and weighted mode) and multiple sensitivity analyses (heterogeneity analysis, leave‐one‐out sensitivity analysis, and horizontal pleiotropy analysis) were employed to evaluate the impact of epigenetic age on HF and vice versa. Bidirectional MR analysis of two samples revealed that the epigenetic PhenoAge clock increased the risk of HF [IVW odds ratio (OR) 1.015, 95% confidence interval (CI) 1.002–1.028, P = 0.028 and weighted median OR 1.020, 95% CI 1.001–1.038, P = 0.039]. Other results were not statistically significant. Conclusions The bidirectional MR analysis demonstrated a causal link between genetically predicted epigenetic age and HF in individuals of European descent. Further research into epigenetic age in other populations and additional genetic information related to HF is warranted.
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- 2023
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46. Roles of Two-Dimensional Materials in Antibiofilm Applications: Recent Developments and Prospects
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Lei Xin, Hongkun Zhao, Min Peng, and Yuanjie Zhu
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biofilm ,two-dimensional nanomaterials ,MoS2 ,MXene ,black phosphorus ,photothermal therapy (PTT) ,Medicine ,Pharmacy and materia medica ,RS1-441 - Abstract
Biofilm-associated infections pose a significant challenge in healthcare, constituting 80% of bacterial infections and often leading to persistent, chronic conditions. Conventional antibiotics struggle with efficacy against these infections due to the high tolerance and resistance induced by bacterial biofilm barriers. Two-dimensional nanomaterials, such as those from the graphene family, boron nitride, molybdenum disulfide (MoS2), MXene, and black phosphorus, hold immense potential for combating biofilms. These nanomaterial-based antimicrobial strategies are novel tools that show promise in overcoming resistant bacteria and stubborn biofilms, with the ability to circumvent existing drug resistance mechanisms. This review comprehensively summarizes recent developments in two-dimensional nanomaterials, as both therapeutics and nanocarriers for precision antibiotic delivery, with a specific focus on nanoplatforms coupled with photothermal/photodynamic therapy in the elimination of bacteria and penetrating and/or ablating biofilm. This review offers important insight into recent advances and current limitations of current antibacterial nanotherapeutic approaches, together with a discussion on future developments in the field, for the overall benefit of public health.
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- 2024
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47. Ecological risks of high-ammonia environment with inhibited growth of Daphnia magna: Disturbed energy metabolism and oxidative stress
- Author
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Ma, Yunfeng, Liu, Yanhua, Sun, Jiawei, Min, Peng, Liu, Wei, Li, Lei, Yi, Pan, Guo, Ruixin, and Chen, Jianqiu
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- 2024
- Full Text
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48. In-situ physical/chemical cross-linked hydrogel electrolyte achieving ultra-stable zinc anode-electrolyte interface towards dendrite-free zinc ion battery
- Author
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Li, Chen-Yang, Wang, Jiang-Lin, Zhang, Dong-Ting, Li, Min-Peng, Chen, Hao, Yi, Wei-Hai, Ren, Xin-Ying, Liu, Bao, Lu, Xue-Feng, and Liu, Mao-Cheng
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- 2024
- Full Text
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49. The FinBen: An Holistic Financial Benchmark for Large Language Models.
- Author
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Qianqian Xie, Weiguang Han, Zhengyu Chen, Ruoyu Xiang, Xiao Zhang, Yueru He, Mengxi Xiao, Dong Li, Yongfu Dai, Duanyu Feng, Yijing Xu, Haoqiang Kang, Ziyan Kuang, Chenhan Yuan, Kailai Yang, Zheheng Luo, Tianlin Zhang, Zhiwei Liu, Guojun Xiong, Zhiyang Deng, Yuechen Jiang, Zhiyuan Yao, Haohang Li, Yangyang Yu, Gang Hu, Jiajia Huang, Xiao-Yang Liu, Alejandro Lopez-Lira, Benyou Wang, Yanzhao Lai, Hao Wang, Min Peng 0002, Sophia Ananiadou, and Jimin Huang
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- 2024
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50. No Language is an Island: Unifying Chinese and English in Financial Large Language Models, Instruction Data, and Benchmarks.
- Author
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Gang Hu, Ke Qin, Chenhan Yuan, Min Peng 0002, Alejandro Lopez-Lira, Benyou Wang, Sophia Ananiadou, Wanlong Yu, Jimin Huang, and Qianqian Xie
- Published
- 2024
- Full Text
- View/download PDF
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