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211 results on '"Miller, Ann L."'

5. Stabilizing membrane domains antagonizes n-alcohol anesthesia

Author

Machta, Benjamin B., Gray, Ellyn, Nouri, Mariam, McCarthy, Nicola L. C., Gray, Erin M., Miller, Ann L., Brooks, Nicholas J., and Veatch, Sarah L.

Subjects
Physics - Biological Physics, Condensed Matter - Soft Condensed Matter, Quantitative Biology - Cell Behavior, Quantitative Biology - Neurons and Cognition
Abstract

Diverse molecules induce general anesthesia with potency strongly correlated both with their hydrophobicity and their effects on certain ion channels. We recently observed that several n-alcohol anesthetics inhibit heterogeneity in plasma membrane derived vesicles by lowering the critical temperature ($T_c$) for phase separation. Here we exploit conditions that stabilize membrane heterogeneity to further test the correlation between the anesthetic potency of n-alcohols and effects on $T_c$. First we show that hexadecanol acts oppositely to n-alcohol anesthetics on membrane mixing and antagonizes ethanol induced anesthesia in a tadpole behavioral assay. Second, we show that two previously described `intoxication reversers' raise $T_c$ and counter ethanol's effects in vesicles, mimicking the findings of previous electrophysiological and behavioral measurements. Third, we find that hydrostatic pressure, long known to reverse anesthesia, also raises $T_c$ in vesicles with a magnitude that counters the effect of butanol at relevant concentrations and pressures. Taken together, these results demonstrate that $\Delta T_c$ predicts anesthetic potency for n-alcohols better than hydrophobicity in a range of contexts, supporting a mechanistic role for membrane heterogeneity in general anesthesia., Comment: 20 pages, 6 figures

Published
2016
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18. ZnUMBA – a live imaging method to detect local barrier breaches.

Author

Higashi, Tomohito, Stephenson, Rachel E., Schwayer, Cornelia, Huljev, Karla, Higashi, Atsuko Y., Heisenberg, Carl-Philipp, Chiba, Hideki, and Miller, Ann L.

Subjects
TIGHT junctions, EPITHELIAL cells, DYNAMICAL systems, MONOMOLECULAR films, XENOPUS, CLAUDINS
Abstract

Epithelial barrier function is commonly analyzed using transepithelial electrical resistance, which measures ion flux across a monolayer, or by adding traceable macromolecules and monitoring their passage across the monolayer. Although these methods measure changes in global barrier function, they lack the sensitivity needed to detect local or transient barrier breaches, and they do not reveal the location of barrier leaks. Therefore, we previously developed a method that we named the zinc-based ultrasensitive microscopic barrier assay (ZnUMBA), which overcomes these limitations, allowing for detection of local tight junction leaks with high spatiotemporal resolution. Here, we present expanded applications for ZnUMBA. ZnUMBA can be used in Xenopus embryos to measure the dynamics of barrier restoration and actin accumulation following laser injury. ZnUMBA can also be effectively utilized in developing zebrafish embryos as well as cultured monolayers of Madin–Darby canine kidney (MDCK) II epithelial cells. ZnUMBA is a powerful and flexible method that, with minimal optimization, can be applied to multiple systems to measure dynamic changes in barrier function with spatiotemporal precision [ABSTRACT FROM AUTHOR]

Published
2023
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30. Rho and F-actin self-organize within an artificial cell cortex

Author

Landino, Jennifer, primary, Leda, Marcin, additional, Michaud, Ani, additional, Swider, Zachary T., additional, Prom, Mariah, additional, Field, Christine M., additional, Bement, William M., additional, Vecchiarelli, Anthony G., additional, Goryachev, Andrew B., additional, and Miller, Ann L., additional

Published
2021
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31. Rho GTPase activity zones and transient contractile arrays

Author

Bement, William M., Miller, Ann L., and von Dassow, George

Subjects
Contractility (Biology) -- Research, Cytokinesis -- Research, GTP -- Research, Hydrolysis -- Analysis, Biological sciences
Abstract

Rho GTPases can be activated in subcellular zones that appear semi-stable, yet are dynamically maintained. The basic features of Rho GTPases activity zones are reviewed, and the implications of zone properties from the perspective of both their function and how they are likely to be controlled are discussed.

Published
2006

32. The Abl-related gene (Arg) requires its F-actinmicrotubule cross-linking activity to regulate lamellipodial dynamics during fibroblast adhesion

Author

Miller, Ann L., Wang, Yinxiang, Mooseker, Mark S., and Koleske, Anthony J.

Subjects
Cytology -- Research, Biological sciences
Abstract

Microtubules (MTs) help establish and maintain cell polarity by promoting actin-dependent membrane protrusion at the leading edge of the cell, but the molecular mechanisms that mediate cross-talk between actin and MTs during this process are unclear. We demonstrate that the Abl-related gene (Arg) nonreceptor tyrosine kinase is required for dynamic lamellipodial protrusions after adhesion to fibronectin, [arg.sup.-/-] fibroblasts exhibit reduced lamellipodial dynamics as compared with wild-type fibroblasts, and this defect can be rescued by reexpression of an Arg-yellow fluorescent protein fusion. We show that Arg can bind MTs with high affinity and cross-link filamentous actin (F-actin) bundles and MTs in vitro. MTs concentrate and insert into Arg-induced F-actin-rich cell protrusions. Arg requires both its F-actin-binding domains and its MT-binding domain to rescue the defects in lamellipodial dynamics of [arg.sup.-/-] fibroblasts. These findings demonstrate that Arg can mediate physical contact between F-actin and MTs at the cell periphery and that this cross-linking activity is required for Arg to regulate lamellipodial dynamics in fibroblasts.

Published
2004

35. Activator-inhibitor coupling between Rho signalling and actin assembly makes the cell cortex an excitable medium

Author

Goryachev, Andrew, Leda, Marcin, Miller, Ann L., von Dassow, George, and Bement, William M

Subjects
rho GTP-Binding Proteins, Rho zone, excitable dynamics, cytokinesis, macromolecular substances, Actins, F-actin, traveling waves, activator-inhibitor coupling, Commentary, Ect2, guanine nucleotide exchange factor, Animals, Humans, Rho small GTPases
Abstract

Emergence of the cytokinetic Rho zone that orchestrates formation and ingression of the cleavage furrow had been explained previously via microtubule-dependent cortical concentration of Ect2, a guanine nucleotide exchange factor for Rho. The results of a recent publication now demonstrate that, en route from resting cortex to fully established furrow, there lies a regime of cortical excitability in which Rho activity and F-actin play the roles of the prototypical activator and inhibitor, respectively. This cortical excitability is manifest as dramatic traveling waves on the cortex of oocytes and embryos of frogs and starfish. These waves are initiated by autocatalytic activation of Rho at the wave front and extinguished by F-actin-dependent inhibition at their back. It is still unclear how propagating excitable Rho-actin waves give rise to the stable co-existence of Rho activity and F-actin density in the static cleavage furrow during cytokinesis. It is possible that some central spindle-associated signaling molecule simply turns off the inhibition of Rho activity by F-actin. However, mathematical modeling suggests a distinct scenario in which local “re-wiring” of the Rho-actin coupling in the furrow is no longer necessary. Instead, the model predicts that the continuously rising level of Ect2 produces in the furrow a qualitatively new stable steady state that replaces excitability and brings about the stable co-existence of high Rho activity and dense F-actin despite the continuing inhibition of Rho by F-actin.

Published
2016
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37. SGEF forms a complex with Scribble and Dlg1 and regulates epithelial junctions and contractility

Author

Awadia, Sahezeel, primary, Huq, Farah, additional, Arnold, Torey R., additional, Goicoechea, Silvia M., additional, Sun, Young Joo, additional, Hou, Titus, additional, Kreider-Letterman, Gabriel, additional, Massimi, Paola, additional, Banks, Lawrence, additional, Fuentes, Ernesto J., additional, Miller, Ann L., additional, and Garcia-Mata, Rafael, additional

Published
2019
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42. Author Correction: A novel atypical sperm centriole is functional during human fertilization

Author

Fishman, Emily L., primary, Jo, Kyoung, additional, Nguyen, Quynh P. H., additional, Kong, Dong, additional, Royfman, Rachel, additional, Cekic, Anthony R., additional, Khanal, Sushil, additional, Miller, Ann L., additional, Simerly, Calvin, additional, Schatten, Gerald, additional, Loncarek, Jadranka, additional, Mennella, Vito, additional, and Avidor-Reiss, Tomer, additional

Published
2018
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43. A novel atypical sperm centriole is functional during human fertilization

Author

Fishman, Emily L., primary, Jo, Kyoung, additional, Nguyen, Quynh P. H., additional, Kong, Dong, additional, Royfman, Rachel, additional, Cekic, Anthony R., additional, Khanal, Sushil, additional, Miller, Ann L., additional, Simerly, Calvin, additional, Schatten, Gerald, additional, Loncarek, Jadranka, additional, Mennella, Vito, additional, and Avidor-Reiss, Tomer, additional

Published
2018
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