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2. Risk profiles and one-year outcomes of patients with newly diagnosed atrial fibrillation in India: Insights from the GARFIELD-AF Registry
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Kakkar, Ajay K., Bassand, Jean-Pierre, John Camm, A., Fitzmaurice, David A., Goldhaber, Samuel Z., Goto, Shinya, Haas, Sylvia, Hacke, Werner, Mantovani, Lorenzo G., Misselwitz, Frank, Pieper, Karen S., Turpie, Alexander G.G., van Eickels, Martin, Verheugt, Freek W.A., Kayani, Gloria, Fox, Keith A.A., Gersh, Bernard J., Luciardi, Hector Lucas, Gibbs, Harry, Brodmann, Marianne, Cools, Frank, Barretto, Antonio Carlos Pereira, Connolly, Stuart J., Spyropoulos, Alex, Eikelboom, John, Corbalan, Ramon, Hu, Dayi, Jansky, Petr, Nielsen, Jørn Dalsgaard, Ragy, Hany, Raatikainen, Pekka, Le Heuzey, Jean-Yves, Darius, Harald, Keltai, Matyas, Kakkar, Sanjay, Sawhney, Jitendra Pal Singh, Agnelli, Giancarlo, Ambrosio, Giuseppe, Koretsune, Yukihiro, Sánchez Díaz, Carlos Jerjes, Ten Cate, Hugo, Atar, Dan, Stepinska, Janina, Panchenko, Elizaveta, Lim, Toon Wei, Jacobson, Barry, Oh, Seil, Viñolas, Xavier, Rosenqvist, Marten, Steffel, Jan, Angchaisuksiri, Pantep, Oto, Ali, Parkhomenko, Alex, Al Mahmeed, Wael, Fitzmaurice, David, Hu, D.Y., Chen, K.N., Zhao, Y.S., Zhang, H.Q., Chen, J.Z., Cao, S.P., Wang, D.W., Yang, Y.J., Li, W.H., Yin, Y.H., Tao, G.Z., Yang, P., Chen, Y.M., He, S.H., Wang, Ying, Wang, Yong, Fu, G.S., Li, X., Wu, T.G., Cheng, X.S., Yan, X.W., Zhao, R.P., Chen, M.S., Xiong, L.G., Chen, P., Jiao, Y., Guo, Y., Xue, L., Wang, F.Z., Li, H., Yang, Z.M., Bai, C.L., Chen, J., Chen, J.Y., Chen, X., Feng, S., Fu, Q.H., Gao, X.J., Guo, W.N., He, R.H., He, X.A., Hu, X.S., Huang, X.F., Li, B., Li, J., Li, L., Li, Y.H., Liu, T.T., Liu, W.L., Liu, Y.Y., Lu, Z.C., Luo, X.L., Ma, T.Y., Peng, J.Q., Sheng, X., Shi, X.J., Sun, Y.H., Tian, G., Wang, K., Wang, L., Wu, R.N., Xie, Q., Xu, R.Y., Yang, J.S., Yang, L.L., Yang, Q., Ye, Y., Yu, H.Y., Yu, J.H., Yu, T., Zhai, H., Zhan, Q., Zhang, G.S., Zhang, Q., Zhang, R., Zhang, Y., Zheng, W.Y., Zhou, B., Zhou, Z.H., Zhu, X.Y., Kakkar, S., Sawhney, J.P.S., Jadhav, P., Durgaprasad, R., Ravi Shankar, A.G., Rajput, R.K., Bhargava, K., Sarma, R., Srinivas, A., Roy, D., Nagamalesh, U.M., Chopda, M., Kishore, R., Kulkarni, G., Chandwani, P., Pothiwala, R.A., Padinhare Purayil, M., Shah, S., Chawla, K., Kothiwale, V.A., Raghuraman, B., Vijayaraghavan, G., Vijan, V.M., Bantwal, G., Bisne, V., Khan, A., Gupta, J.B., Kumar, S., Jain, D., Abraham, S., Adak, D., Barai, A., Begum, H., Bhattacharjee, P., Dargude, M., Davies, D., Deshpande, B., Dhakrao, P., Dhyani, V., Duhan, S., Earath, M., Ganatra, A., Giradkar, S., Jain, V., Karthikeyan, R., Kasala, L., Kaur, S., Krishnappa, S., Lawande, A., Lokesh, B., Madarkar, N., Meena, R., More, P., Naik, D., Prashanth, K., Rao, M., Rao, N.M., Sadhu, N., Shah, D., Sharma, M., Shiva, P., Singhal, S., Suresh, S., Vanajakshamma, V., Panse, S.G., Koretsune, Y., Kanamori, S., Yamamoto, K., Kumagai, K., Katsuda, Y., Sadamatsu, K., Toyota, F., Mizuno, Y., Misumi, I., Noguchi, H., Ando, S., Suetsugu, T., Minamoto, M., Oda, Hiroshi, Shiraishi, K., Adachi, S., Chiba, K., Norita, H., Tsuruta, M., Koyanagi, T., Ando, H., Higashi, T., Okada, K., Azakami, S., Komaki, S., Kumeda, K., Murayama, T., Matsumura, J., Oba, Y., Sonoda, R., Goto, K., Minoda, K., Haraguchi, Y., Suefuji, H., Miyagi, H., Kato, H., Nakamura, Tadashi, Nakamura, Tsugihiro, Nandate, H., Zaitsu, R., Fujiura, Yoshihisa, Yoshimura, A., Numata, H., Ogawa, J., Tatematsu, H., Kamogawa, Y., Murakami, K., Wakasa, Y., Yamasawa, M., Maekawa, H., Abe, S., Kihara, H., Tsunoda, S., Saito, Katsumi, Saito, Kazuyuki, Fudo, T., Obunai, K., Tachibana, H., Oba, I., Kuwahata, T., Higa, S., Gushiken, M., Eto, T., Yoshida, H., Ikeda, D., Fujiura, Yoshitake, Ishizawa, M., Nakatsuka, M., Murata, K., Ogurusu, C., Shimoyama, M., Akutsu, M., Takamura, I., Hoshino, F., Yokota, N., Iwao, T., Tsuchida, K., Takeuchi, M., Hatori, Y., Kitami, Y., Nakamura, Yoichi, Oyama, R., Ageta, M., Oda, Hiroyuki, Go, Y., Mishima, K., Unoki, T., Morii, S., Shiga, Yuhei, Sumi, H., Nagatomo, T., Sanno, K., Fujisawa, K., Atsuchi, Y., Nagoshi, T., Seto, T., Tabuchi, T., Kameko, M., Nii, K., Oshiro, K., Takezawa, H., Nagano, S., Miyamoto, N., Iwaki, M., Nakamura, Yuichiro, Fujii, M., Okawa, M., Abe, Masahiko, Abe, Masatake, Abe, Mitsunori, Saito, T., Mito, T., Nagao, K., Minami, J., Mita, T., Sakuma, I., Taguchi, T., Marusaki, S., Doi, H., Tanaka, M., Fujito, T., Matsuta, M., Kusumoto, T., Kakinoki, S., Ashida, K., Yoshizawa, N., Agata, J., Arasaki, O., Manita, M., Ikemura, M., Fukuoka, S., Murakami, H., Matsukawa, S., Hata, Y., Taniguchi, T., Ko, T., Kubo, H., Imamaki, M., Akiyama, M., Inagaki, M., Odakura, H., Ueda, T., Katsube, Y., Nakata, A., Watanabe, H., Techigawara, M., Igarashi, M., Taga, K., Kimura, T., Tomimoto, S., Shibuya, M., Nakano, M., Ito, K., Seo, T., Hiramitsu, S., Hosokawa, H., Hoshiai, M., Hibino, M., Miyagawa, K., Horie, Hajime, Sugishita, N., Shiga, Yukio, Soma, A., Neya, K., Yoshida, Tetsuro, Yoshida, Tomoki, Mizuguchi, M., Ishiguro, M., Minagawa, T., Wada, M., Mukawa, H., Okuda, F., Nagasaka, S., Abe, Y., Adachi, Sen, Adachi, 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T., Maeda, I., Maeda, K., Maruyama, Y., Matsui, S., Matsushita, K., Matsuura, Y., Mineoi, K., Mitsuhashi, H., Miura, N., Miyaguchi, S., Miyajima, S., Miyamoto, H., Miyashita, A., Miyata, S., Mizuguchi, I., Mizuno, A., Mori, T., Moriai, O., Morishita, K., Murai, O., Nagai, Sho, Nagai, Shunichi, Nagata, E., Nagata, H., Nakagomi, A., Nakahara, S., Nakamura, M., Nakamura, R., Nakanishi, N., Nakayama, T., Nakazato, R., Nanke, T., Nariyama, J., Niijima, Y., Niinuma, H., Nishida, Y., Nishihata, Y., Nishino, K., Nishioka, H., Nishizawa, K., Niwa, I., Nomura, K., Nomura, S., Nozoe, M., Ogawa, T., Ohara, N., Okada, M., Okamoto, K., Okita, H., Okuyama, M., Ono, H., Ono, T., Onuki Pearce, Y., Oriso, S., Ota, A., Otaki, E., Saito, Y., Sakai, H., Sakamoto, N., Sakamoto, Y., Samejima, Y., Sasagawa, Y., Sasaguri, H., Sasaki, A., Sasaki, T., Sato, Kazuki, Sato, Kiyoharu, Sawano, M., Seki, S., Sekine, Y., Seta, Y., Sezaki, K., Shibata, N., Shiina, Y., Shimono, H., Shimoyama, Y., Shindo, T., Shinohara, H., Shinohe, R., Shinozuka, T., Shirai, T., Shiraiwa, T., Shozawa, Y., Suga, T., Sugimoto, C., Suzuki, Kazuo, Suzuki, Keita, Suzuki, Shu, Suzuki, Shunji, Suzuki, Susumu, Suzuki, Y., Tada, M., Taguchi, A., Takagi, T., Takagi, Y., Takahashi, K., Takahashi, S., Takai, H., Takanaka, C., Take, S., Takeda, H., Takei, K., Takenaka, K., Tana, T., Tanabe, G., Taya, K., Teragawa, H., Tohyo, S., Toru, S., Tsuchiya, Y., Tsuji, T., Tsuzaki, K., Uchiyama, H., Ueda, O., Ueyama, Y., Wakaki, N., Wakiyama, T., Washizuka, T., Watanabe, M., Yamada, T., Yamagishi, T., Yamaguchi, H., Yamamoto, Kenichi, Yamamoto, Kentaro, Yamamoto, Kunihiko, Yamamoto, T., Yamaura, M., Yamazoe, M., Yasui, K., Yokoyama, Y., Yoshida, K., Lim, T.W., Ching, C.K., Foo, C.G., Chow, J.H., Chen, D.D., Jaufeerally, F.R., Lee, Y.M., Lim, G., Lim, W.T., Thng, S., Yap, S.Y., Yeo, C., Oh, S., Pak, H.N., Kim, J.-B., Kim, J.H., Jang, S.-W., Kim, D.H., Ryu, D.R., Park, S.W., Kim, D.-K., Choi, D.J., Oh, Y.S., Cho, M.-C., Kim, S.-H., Jeon, H.-K., Shin, D.-G., Park, J.S., Park, H.K., Han, S.-J., Sung, J.H., Cho, J.-G., Nam, G.-B., On, Y.K., Lim, H.E., Kwak, J.J., Cha, T.-J., Hong, T.J., Park, S.H., Yoon, J.H., Kim, N.-H., Kim, K.-S., Jung, B.C., Hwang, G.-S., Kim, C.-J., Kim, D.B., Ahn, J.J., An, H.J., Bae, H., Baek, A.L., Chi, W.J., Choi, E.A., Choi, E.H., Choi, H.K., Choi, H.S., Han, S., Heo, E.S., Her, K.O., Hwang, S.W., Jang, E.M., Jang, H.-S., Jang, S., Jeon, H.-G., Jeon, S.R., Jeon, Y.R., Jeong, H.K., Jung, I.-A., Kim, Hyeon Jeong, Kim, Hyun Ju, Kim, Ji Seon, Kim, Jung Sook, Kim, J.A., Kim, K.T., Kim, M.S., Kim, Sang Hee, Kim, Sang Hyun, Kim, Y.-I., Lee, C.S., Lee, E.H., Lee, G.H., Lee, H.Y., Lee, H.-Y., Lee, K.H., Lee, K.R., Lee, M.S., Lee, M.-Y., Lee, R.W., Lee, S.E., Lee, S.H., Lee, S., Lee, W.Y., Noh, I.K., Park, A.R., Park, B.R., Park, H.N., Park, J.H., Park, M., Park, Y., Seo, S.-Y., Shim, J., Sim, J.H., Sohn, Y.M., Son, W.S., Son, Y.S., Song, H.J., Wi, H.K., Woo, J.J., Ye, S., Yim, K.H., Yoo, K.M., Yoon, E.J., Yun, S.Y., Angchaisuksiri, P., Chawanadelert, S., Mongkolwongroj, P., Kanokphatcharakun, K., Cheewatanakornkul, S., Laksomya, T., Pattanaprichakul, S., Chantrarat, T., Rungaramsin, S., Silaruks, S., Wongcharoen, W., Siriwattana, K., Likittanasombat, K., Katekangplu, P., Boonyapisit, W., Cholsaringkarl, D., Chatlaong, B., Chattranukulchai, P., Santanakorn, Y., Hutayanon, P., Khunrong, P., Bunyapipat, T., Jai-Aue, S., Kaewsuwanna, P., Bamungpong, P., Gunaparn, S., Hongsuppinyo, S., Inphontan, R., Khattaroek, R., Khunkong, K., Kitmapawanont, U., Kongsin, C., Naratreekoon, B., Ninwaranon, S., Phangyota, J., Phrommintikul, A., Phunpinyosak, P., Pongmorakot, K., Poomiphol, S., Pornnimitthum, N., Pumprueg, S., Ratchasikaew, S., Sanit, K., Sawanyawisuth, K., Silaruks, B., Sirichai, R., Sriwichian, A., Suebjaksing, W., Sukklad, P., Suttana, T., Tangsirira, A., Thangpet, O., Tiyanon, W., Vorasettakarnkij, Y., Wisaratapong, T., Wongtheptien, W., Wutthimanop, A., Yawila, S., Oto, A., Altun, A., Ozdogru, I., Ozdemir, K., Yilmaz, O., Aydinlar, A., Yilmaz, M.B., Yeter, E., Ongen, Z., Cayli, M., Pekdemir, H., Ozdemir, M., Sucu, M., Sayin, T., Demir, M., Yorgun, H., Ersanli, M., Okuyan, E., Aras, D., Abdelrahman, H., Aktas, O., Alpay, D., Aras, F., Bireciklioglu, M.F., Budeyri, S., Buyukpapuc, M., Caliskan, S., Esen, M., Felekoglu, M.A., Genc, D., Ikitimur, B., Karaayvaz, E.B., Kılıç Karataş, S., Okutucu, S., Ozcelik, E., Quisi, A., Sag, H., Sahiner, L., Sayin, B.Y., Seker, T., Uzun Alkan, D., Yildirim, E., Yildirim, R., Yilmaz, F., Yuksekdag, V., Luciardi, H.L., Vensentini, N., Ingaramo, A.C., Sambadaro, G.A., Fernandez Caputi, V., Berman, S.G., Dragotto, P., Kleiban, A.J., Centurion, N., Giacomi, G., Ahuad Guerrero, R.A., Conde, D., Zapata, G., Di Paola, L.A., Ramos, J.L., Dran, R.D., Egido, J., Fernandez, A.A., Fosco, M.J., Sassone, S., Sinisi, V.A., Cartasegna, L.R., Berli, M.A., Gomez Vilamajo, O.A., Ferroni, F., Alaguibe, E.D., Alvarez D'Amelio, A., Arabetti, C., Arias, L., Belardi, J.A., Bergesio, L., Berli, F., Berli, M., Borchowiec, S., Buzzetti, C., Cabrini, R., Campisi, V., Cappi, A.L., Carrizo, R., Colombo Berra, F., Costabel, J.P., Costamagna, O.J.A., Damonte, A.A., De Urquiza, I.N., Diez, F., Edén, M.F., Fanuele, M., Fernandez Voena, F., Foa Torres, M., Funosas, C., Giacomi, M.P., Gimenez, C.H., Gurfinkel, E.P., de L.M. Had, M., Hansen, V., Hrabar, A.D., Ingratta, M., Lopez, A., Maehara, G., Maffei, L., Martinelli, A., Martinelli, C., Matkovich, J., Mautner, B., Meirino, A., Munguia, R., Navarro, A., Novas, V., Perez Prados, G., Pontoriero, J., Potito, R.N., Ricotti, C., Rodriguez, M.A., Rolandi, F., Said Palladino, M.E., Salinger, M., Sanziani, L.S., Schygiel, P.O., Sossich, A., Tinto, J.F., Tonelli, L., Tufare, A.L., Vallejo, M., Yunis, M.E., Zillo, M., Zurbrigk, F.J., Barretto, A.C.P., Sobral Filho, D.C., Jaber, J., Armaganijan, D., Faria Neto, J., Steffens, A., Kunz Sebba Barroso de Souza, W., de Souza Neto, J.D., Ribeiro, J.M., Silveira Teixeira, M., Ferreira Rossi, P.R., Pires, L., Moreira, D., Moura Jorge, J.C., Menezes Lorga Filho, A., Bodanese, L.C., Westerlund Montera, M., Del Carlo, C.H., Da Rocha Rodrigues, T., Alves da Costa, F.A., Lopes, A., Lopes, R., Araújo, G.R., Fernandes Manenti, E.R., Kerr Saraiva, J.F., Ferreira Braga, J.C., Negri, A., Souto, L., Moncada, C., Bertolim Precoma, D., Roquette, F., Reis, G., Ramos Filho, R.A., Lanna Figueiredo, E., Vieira Botelho, R., Munhoz da Fontoura Tavares, C., Costantini Frack, C.R., Abdalla Saad, J., Finimundi, H.C., Pisani, C., Chemello, D., Pereira Martins, M., Broilo França, C.C., Alban, F., Aranha Rosito, G.B., de Moura Xavier Moraes Junior, J.B., Tumelero, R.T., Nigro Maia, L., Simões de Almeida, R., do Carmo Borges, N.C., Gomes Ferreira, L.G., Agliardi, P., Alves de Oliveira Gomes, J., Araujo, V., Arruda Nakazone, M., Barbosa, T., Barroso, S., Belisario Falchetto, E., Bellotti Lopes, H., Benez Teixeira Lemos, M.A., Biazus, G., Borges Queiroz, L., Camazzola, F.E., Caporale, M., Cardoso Boscato, S., Chieza, F., Chokr, M.O., Clemente Mingireanov, R., Codonho Góes, N., Correa, C., Costa, M., Costantini Ortiz, C., da Silva, L.S., da Silva Paulitsch, F., da Silveira, J.A., Daros, E., de Araújo, G.R., Del Monaco, M.I., Dias, C., Dias, M.A., Drummond Wainstein, A.P., Ely Pizzato, P., Esteves, D.C., Fabri, P., Félix Lorenzato Fonseca, T., Fernandes, E., Fonseca, C., Frack Costantini, C.R., Franchin Ferraz, R., Freire, F., Gottardo, P., Guanaes, D., Guizzardi, S., Hettwer Magedanz, E., Igansi, F., Jannuzzi, F., Junior, G., Komar, D., Lino, E.G., Lopes, D., Lourenço da Silva Júnior, O., Lustosa, E., Macagnan, A.P., Marinho, M.C., Mazzoni, M., Melo, G., Mortari, L., Mouco, O.M.C.C., Nanzer Vital, C., Ormundo, C., Oss Emmer, S., Palmegiani, E., Pavani, R., Pereira, L., Pereira, V.L., Perreira, R., Poletti, S., Quaia Fortunato, S.C., Queirantes, C., Ramos Pereira, N., Rech, R.L., Ribeiro, S., Rodrigues, A., Roesch, H., Ruaro Reichert, T., Santos, D., Santos, I., Santos, M., Seroqui, M.V., Silva, S., Soares, L., Spolaor, L., Stoll, C., Toazza Duda, N., Trama, L., Unterkircher, B., Valois, M.V., Vargas, T., Viana, T., Vicente, C., Vidal Armaganijan, L., Vieira Homem, R., Vieira Torres, L.G., Vila Boas, L., Villaça Guimarães Filho, F., Corbalan, R., Eggers, G., Bugueño Gutiérrez, C., Arriagada, G., Potthoff Cardenas, S., Stockins Fernandez, B.A.J., Conejeros, C., Houzvic, C., Marin Cuevas, P., Montecinos, H., Forero, A., Lanas, F., Larico Gómez, M., Charme Vilches, G., Rey, C., Astudillo, C., Aguilar, J., Campisto, Y., Lara, C., Molina, E., Munoz Oyarzon, J., Olguin, V., Vergara, M., Villan, C., Sánchez Díaz, C.J., Illescas Diaz, J., Leal Cantú, R., Ramos Zavala, M.G., Cabrera Jardines, R., Espinola Zavaleta, N., Villarreal Umaña, S., López Rosas, E., Llamas Esperón, G., Pozas, G., Cardona Muñoz, E., Matadamas Hernández, N., Leyva Rendón, A., García Hernández, N., de los Ríos Ibarra, M., Virgen Carrillo, L., López Villezca, D., Hernández Herrera, C., López Prieto, J.J., Gaona Rodríguez, R., Villeda Espinosa, E., Flores Martínez, D., Velasco Barcena, J., Yong, R., Rodríguez Briones, I., Leiva Pons, J.L., Álvarez López, H., Olvera Ruiz, R., Díaz de la Vega, C., Cantú Brito, C., Chuquiure Valenzuela, E., Reyes-Sanchez, R., Bazzoni Ruiz, A., Nandayapa Flores, O., Benavides Gonzalez, M., 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N., Goodwin, D., Saunders, P., Evans, R., Leese, J., Jhittay, P.S., Ross, A., Kainth, M.S., Pickavance, G., McDonnell, J., Williams, A., Gooding, T., Wagner, H., Suryani, S., Singal, A., Sircar, S., Bilas, R., Hutchinson, P., Wakeman, A., Stokes, M., Paul, N., Aziz, M., Ramesh, C., Wilson, P., Franklin, S., Fairhead, S., Thompson, J., St Joseph, V., Taylor, G., Tragen, D., Seamark, D., Paul, C., Richardson, M., Jefferies, A., Sharp, H., Jones, H., Giles, C., Page, M., Oginni, O., Aldegather, J., Wetherwell, S., Lumb, W., Evans, P., Scouller, F., Macey, N., Stipp, Y., West, R., Thurston, S., Wadeson, P., Matthews, J., Pandya, P., Gallagher, A., Railton, T., Sinha, B., Russell, D., Davies, J.A., Ainsworth, P., Jones, C.P., Weeks, P., Eden, J., Kernick, D., Murdoch, W., Lumley, L., Patel, R.P., Wong, S.W., Saigol, M., Ladha, K., Douglas, K., Cumberlidge, D.F., Bradshaw, C., Van Zon, G., Jones, K.P., Thomas, M.J., Watson, E., Sarai, B., Ahmad, N., Willcock, W., Cairns, J., Sathananthan, S., de Kare-Silver, N., Gilliland, A., Strieder, E., Howitt, A., Vishwanathan, B., Bird, N., Gray, D., Clark, M., Bisatt, J., Litchfield, J., Fisher, E., Fooks, T., Kelsall, A.R., Alborough, E., Wakeling, J., Parfitt, M., Milne, K., Rogers, S., Priyadharshan, R., Oliver, J.L., Davies, E., Abushal, S., Jacobs, M., Hutton, C., Walls, N.I., Thompson, R., Chigbo, C., Zaidi, S.M.A., Howard, M., Butter, K.C., Barrow, S., Little, H., Haq, I.U., Gibbons, L., Glencross, S., McLeod, A.J., Poland, K., Mulholland, C., Warke, A., Conn, P., Burns, G., Smith, R.N., Lowe, S., Kamath, R., Dau, H.S., Webster, J., Hodgins, I., Vercoe, S., Roome, P.C., Pinnock, H., Patel, J.R.A., Ali, A., Hart, N., Davies, R., Stuart, E., Neden, C.A., Danielsen, M., Heath, R., Sharma, P., Galloway, S., Hawkins, C., Oliver, R., Aylward, M., Mannion, S., Braddick, M., Edwards, D., Rothwell, A.C., Sabir, A., Choudhary, F., Khalaque, S., Wilson, A., Peters, S., Coulson, W., Roberts, N., Heer, A., Coates, S., Ward, B., Jackson, D., Walton, S., Shepherd, D., Sterry, M., Wong, T., Boon, M., Bunney, R., Haria-Shah, R., Baron, R.T., Davies, S., Schatzberger, T., Hargreaves, N., Stephenson, T., Choi, H., Batson, R., Lucraft, L., Myhill, T., Estifano, S., Geatch, D., Wilkinson, J., Veale, R., Forshaw, K., Davies, T., Zaman, K., Vinson, P., Liley, C., Bandrapalli, M., McGinty, P., Wastling, R., McEleny, P., Beattie, A., Cooke, P., Wong, M., Gunasegaram, J., Pugsley, M., Ahmad, S., A'Court, C., Ayers, J., Bennett, J., Cartwright, S., Dobson, S., Dooldeniya, C., Flynn, A., Fox, R., Goram, J., Halpin, A., Hay, A., Jacobs, P., Jeffers, L., Lomax, L., Munro, I., Muvva, R., Nadaph, M., Powell, K., Randfield, S., Redpath, D., Reed, R., Rickenbach, M., Rogers, G., Saunders, P.B., Seamark, C., Shewring, J., Simmons, P., Simper, H., Stoddart, H., Sword, A., Thomas, N., Thomson, A., Gibbs, H., Blenkhorn, A., Singh, B., Van Gaal, W., Abhayaratna, W., Lehman, R., Roberts-Thomson, P., Kilian, J., Coulshed, D., Catanchin, A., Colquhoun, D., Kiat, H., Eccleston, D., French, J., Zimmett, L., Ayres, B., Phan, T., Blombery, P., Crimmins, D., O'Donnell, D., Choi, A., Astridge, P., Arstall, M., Jepson, N., Binnekamp, M., Lee, A., Rogers, J., Starmer, G., Carroll, P., Faunt, J., Aggarwala, A., Barry, L., Batta, C., Beveridge, R., Black, A., Bonner, M., Boys, J., Buckley, E., Campo, M., Carlton, L., Connelly, A., Conway, B., Cresp, D., Dimitri, H., Dixon, S., Dolman, M., Duroux, M., Eskandari, M., Eslick, R., Ferreira-Jardim, A., Fetahovic, T., Fitzpatrick, D., Geraghty, R., Gibbs, J., Grabek, T., Modi, M.H., Hayes, K., Hegde, M.P., Hesketh, L., Hoffmann, B., Jacobson, B., Johnson, K., Juergens, C., Kassam, I., Lawlor, V., Lehman, M., Lehman, S., Leung, D., Mackay, S., MacKenzie, M., McCarthy, C., McIntosh, C., McKeon, L., Morrison, H., Mussap, C., Myers, J.-D., Nagalingam, V., Oldfield, G., O'May, V., Palmer, J., Parsons, L., Patching, K., Patching, T., Paul, V., Plotz, M., Preston, S., Rashad, H., Ratcliffe, M., Raynes, S., Rose, J., Sanders, L., Seremetkoska, M., Setio, H., Shone, S., Shrestha, P., Singh, C., Singleton, C., Stoyanov, N., Sutcliffe, S., Swaraj, K., Tarrant, J., Thompson, S., Tsay, I.M., Vorster, M., Waldman, A., Wallis, L., Wilford, E., Wong, K., Connolly, S.J., Spyropoulos, A., Eikelboom, J., Luton, R., Gupta, M., Pandey, A.S., Cheung, S., Leader, R., Beaudry, P., Ayala-Paredes, F., Berlingieri, J., Heath, J., Poirier, G., Du Preez, M., Nadeau, R., Dresser, G., Dhillon, R., Hruczkowski, T., Schweitzer, B., Coutu, B., Angaran, P., MacDonald, P., Vizel, S., Fikry, S., Parkash, R., Lavoie, A., Cha, J., Ramjattan, B., Bonet, J., Ahmad, K., Aro, L., Aves, T., Beaudry, K., Bergeron, C., Bigcanoe, J., Bignell, N., Breakwell, L., Burke, E., Carroll, L., Clarke, B., Cleveland, T., Daheb, S., Dehghani, P., Denis, I., Djaidani, Z., Dorian, P., Douglass, S., Dunnigan, J., Ewert, A., Farquhar, D., Fearon, A., Ferleyko, L., Fournier, D., Fox, B., Grenier, M.-C., Gulliver, W., Haveman, K., Hines, C., Hines, K., Jackson, A.M., Jean, C., Jethoo, G., Kahlon, R., Kelly, S., Kim, R., Korley, V., Kornder, J., Kwan, L., Largy, J., Lewis, C., Lewis, S., Mangat, I., Moor, R., Navratil, J., Neas, I., Otis, J., Otis, R., Pandey, M., Petrie, F., Pinter, A., Raines, M., Roberts, P., Robinson, M., Sas, G., Schulman, S., Snell, L., Spearson, S., Stevenson, J., Trahey, T., Wong, S., Wright, D., Ragy, H., Abd El-Aziz, A., Abou Seif, S.K., El Din, M.G., El Etriby, S., Elbahry, A., El-Etreby, A., Elkhadem, M., Katta, A., Khairy, T., Mowafy, A., Nawar, M., Ohanissian, A., Reda, A., Reda, M., Salem, H., Sami, N., Samir, S., Setiha, M., Sobhy, M., Soliman, A., Taha, N., Tawfik, M., Zaatout, E., Kettles, D., Bayat, J., Siebert, H., Horak, A., Kelfkens, Y., Garda, R., Pillay, T., Guerra, M., van Zyl, L., Theron, H., Murray, A., Louw, R., Greyling, D., Mntla, P., Ueckermann, V., Loghdey, R., Ismail, S., Ahmed, F., Engelbrecht, J., Ramdass, A., Maharajh, S., Oosthuysen, W., Angel, G., Bester, C., Booysen, M., Boshoff, C., Cannon, C., Cassimjee, S., Chami, C., Conway, G., Davids, A., de Meyer, L., Du Plessis, G., Ellis, T., Henley, L., Karsten, M., Loyd, E., Marks, J., Mavhusa, L., Mostert, M., Page, A., Rikhotso, L., Salie, M., Sasto, J., Shaik, F., Skein, A., Smith, L., Tarr, G., Tau, T., van Zyl, F., Al Mahmeed, W., Yousef, G., Agrawal, A., Nathani, M., Ibrahim, M., Esheiba, E.M., Singh, R., Naguib, A., Abu-Mahfouz, M., Al Omairi, M., Al Naeemi, A., Maruthanayagam, R., Bazargani, N., Wassef, A., Gupta, R., Khan, M., Subbaraman, B., Abdul, A., Al Mulla, A., El Bardisy, S., Haridas, P., Jadhav, S., Magdaluyo, K., Makdad, M., Maqsood, I., Mohamed, R., Sharma, N., Sharma, R., Thanzeel, M., Goldhaber, S.Z., Canosa, R., Rama, P., Blumberg, E., Garcia, J., Mullen, P., Wilson, V., Quick, A., Ferrick, K., Kutayli, W.M., Cox, M., Franco, M., Falkowski, S., Mendelson, R., Williams, M., Miller, S., Beach, S., Alfieri, A., Gutowski, T., Haque, I., Reddy, R., Ahmed, W., Delafontaine, P., Diercks, D., Theodoro, D., Remmel, K., Alberts, M., Ison, R., Noveck, H., Duffy, P., Pitta, S., Nishijima, D., Treasure, C., Asafu-Adjaye, N., Ball, K., Bartlett, M., Bentley, M., Bowers, S., Brown, A., Browne, A., Cameron-Watts, J., Canova, M., Cassidy, D., Cervellione, K., Congal, S., DePauw, J., Dickerson, A., Eley, M., Evans, L., Felpel, S., Ferdinand, K., Fielder, D., Gentry, P., Haideri, A., Hakimi, F., Harbour, T., Hartranft, E., Hawkins, B., Headlee, M., Henson, L., Herrick, C., Hicks, T., Jasinski, S., Jones, A., Jones, L., Jones, P., Karl, S., Keeling, M., Kerr, J., Knowles, P., Langdon, J., Lay, M., Lee, J.A., Lincoln, T., Malone, E., Merliss, A., Merritt, D., Minardo, J., Mooso, B., Orosco, C., Palumbo, V., Parker, M., Parrott, T., Paserchia, S., Pearl, G., Peterson, J., Pickelsimer, N., Purcell, T., Raynor, J., Raziano, S., Richard, C., Richardson, T., Robertson, C., Sage, A., Sanghera, T., Shaw, P., Shoemaker, J., Smith, K., Stephanie, B., Thatcher, A., Theobald, H., Thompson, N., Treasure, L., Tripti, T., Verdi, C., Worthy, V., Sawhney, Jitendra PS., Kothiwale, Veerappa A., Bisne, Vikas, Durgaprasad, Rajashekhar, Jadhav, Praveen, Chopda, Manoj, Vanajakshamma, Velam, Meena, Ramdhan, Vijayaraghavan, Govindan, Chawla, Kamaldeep, and Allu, Jagan
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- 2018
- Full Text
- View/download PDF
3. Sinorhizobium meliloti RNase III: impact in the dynamics and activity of small non-coding RNAs
- Author
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Guedes-García, S.K., García-Tomsig, Natalia I., Millán, V., Jiménez-Zurdo, José I., Ministerio de Ciencia e Innovación (España), and European Commission
- Abstract
Resumen del poster presentado en: I Spanish-Portuguese Congress on Beneficial Plant-Microbe Interactions (BeMiPlant) and XVIII National Meeting of the Spanish Society of Nitrogen Fixation (XVIII SEFIN). Oeiras, Portugal, 17-19 octubre (2022), This work was supported by grants BFU2017-82645-P and PID2020-114782GB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” (BFU2017-82645-P).
- Published
- 2022
4. Fine-tuning of the Sinorhizobium meliloti nitrogen stress response by the non-coding RNA NfeR1
- Author
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García-Tomsig, Natalia I., García-Fernández, F., Millán, V., Guedes-García, S.K., Robledo, Marta, Jiménez-Zurdo, José I., Ministerio de Ciencia e Innovación (España), and European Commission
- Abstract
Resumen del poster presentado en: I Spanish-Portuguese Congress on Beneficial Plant-Microbe Interactions (BeMiPlant) and XVIII National Meeting of the Spanish Society of Nitrogen Fixation (XVIII SEFIN). Oeiras, Portugal, 17-19 octubre (2022), This work was supported by grants BFU2017-82645-P and PID2020-114782GB-I00 funded by MCIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe” (BFU2017-82645-P).
- Published
- 2022
5. Bacterial community in the rhizosphere of the cactus species Mammillaria carnea during dry and rainy seasons assessed by deep sequencing
- Author
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Torres-Cortés, G., Millán, V., Fernández-González, A. J., Aguirre-Garrido, J. F., Ramírez-Saad, H. C., Fernández-López, M., Toro, N., and Martínez-Abarca, F.
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- 2012
6. Riboregulation of metabolism in nitrogen-fixing symbiotic alpha-rhizobia
- Author
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García-Tomsig, Natalia I., Robledo, Marta, diCenzo, G.C., Mengoni, A., Millán, V., Guedes-García, S.K., and Jiménez-Zurdo, José I.
- Abstract
Resumen de la comunicación oral presentada en: XIII Reunión del Grupo de Microbiología Molecular de La SEM. Granada, 7-9 septiembre (2022), This work was supported by grants BFU2017-82645-P and PID2020-114782GB-I00 funded by MCIN/AEI/ 10.13039/501100011033 and by “ERDF A way of making Europe”.
- Published
- 2022
7. ¿PUEDEN MEJORAR SU PAA LOS ALUMNOS DE ESCASOS RECURSOS? EVIDENCIA EXPERIMENTAL
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Núñez E., Javier and Millán V., Isabel
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- 2002
8. rana súper-pija
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Millán V., Óscar, primary
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- 2020
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- View/download PDF
9. P-07-5 Sexting and New Cybersex Roles Through Social Media
- Author
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Poyato Galán, J.M., primary, Deseare, A., additional, Lozano Blasco, J.M., additional, and García Millán, V., additional
- Published
- 2020
- Full Text
- View/download PDF
10. Functional Characterization of the Sinorhizobium meliloti Nodule Formation Efficiency Non-Coding RNA NfeR1
- Author
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García-Tomsig, Natalia I., Robledo, Marta, Millán, V., Becker, Anke, Jiménez-Zurdo, José I., Ministerio de Economía y Competitividad (España), European Commission, and Agencia Estatal de Investigación (España)
- Abstract
Trabajo presentado en XVII National Meeting of the Spanish Society of Nitrogen Fixation - VI Portuguese-Spanish Congress on Nitrogen Fixation, celebrado en Madrid (España) del 10 al 12 de julio de 2019., Small non-coding RNAs (sRNAs) are ubiquitous components of regulatory netwoks underlying virtually any adaptive response in bacteria. As regulators of gene expression, sRNAs accumulate differentially in the cell in response to external cues and their main activity mechanism involves protein-assisted base-pairing interactions with short and discontinuous stretches of complementary nucleotides usually mapping to the translation initiation region of multiple trans-encoded target mRNAs. In most cases, this antisense interaction promotes post-transcriptional gene silencing by blocking of translation and degradation of the target mRNAs by cellular RNases (reviewed in Jiménez-Zurdo et al. 2013). In our laboratory, we are interested in the characterization of the non-coding transcriptome of the alfalfa symbiont Sinorhizobium meliloti. Deletion of one of the hundreds of sRNA genes identified in this bacterium alters nodule organogenesis and compromises nitrogen-fixation efficiency on alfalfa roots (Robledo et al, 2017). According to this symbiotic phenotype, this sRNA was named NfeR1 (Nodule Formation Efficiency RNA). Computational predictions, some of which have been verified experimentally, suggested that NfeR1 uses redundantly three identical anti-Shine Dalgarno (aSD) motifs for targeting and down-regulation of multiple mRNAs coding for the periplasmic component of ABC transport systems. Northern blot probing and transcription tracking with promoter-eGFP fusions uncovered maximum NfeR1 accumulation upon an osmotic upshift in free-living bacteria and during all steps of the symbiotic interaction. The strength and differential regulation of nfeR1 transcription is conferred by a motif that is conserved in the promoter regions of its ¿-proteobacterial homologs. Pull-down assays revealed that the NfeR1 promoter might bind the global nitrogen regulator NtrC and the LysR type transcriptional regulator LsrB (LysR Symbiotic Regulator). In the communication, we will provide novel insights into the transcriptional regulation, targeting potential, activity mechanism and symbiotic function of NfeR1., Funded by MINECO Feder/EU BFU2013-48282-C2-2-P and MINEICO Feder/EU BFU2017-82645-P
- Published
- 2019
11. ESTIMAÇÃO DO COEFICIENTE DE DIFUSÃO DE GASES EM NANOESTRUTURAS POROSAS DE CARBONO
- Author
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MILLÁN, V, additional and MUNIZ, A. R, additional
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- 2018
- Full Text
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12. Estrategia para la Sostenibilidad Ambiental del Medio Litoral Transfronterizo
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Assunçao, P.C. (Patricia Clemente), Bartolomé-Baraza, A. (Aurora), Betancort, J.R. (Juana Rosa), Bilbao, A. (Alberto), Bonumore, A., Boza-Vindel, C. (Cristina), Carmona, L., Castro, J.J., Cedeño, M.V., Armas, D. (Demetrio) de, Jara, A. de la, Freijanes, K., González, E.L. (Erika Lucía), González-Lorenzo, J.G. (José Gustavo), González, M., González-Gil, S. (Sonsoles), González, Y. (Yurena), Hernández-González, C.L. (Carlos Luis), Jiménez, S. (Sebastián), Lorenzo, A., Martín-Sosa, P. (Pablo), Mendoza, H., Millán, V. (Vanessa), Pérez, Y. (Yeray), Portillo, J.E., Rodrigo, M., Rodríguez, J.E. (Jaime Ezequiel), Santamaría, M.T.G. (María Teresa García), Santan, A., Suárez-Reina, B., Tello-Antón, M.O. (María Olvido), Bilbao, A. (Alberto), Pérez, Y. (Yeray), Betancort, J.R. (Juana Rosa), and Santamaría, M.T.G. (María Teresa García)
- Subjects
Zona litoral ,Gestión integrada ,Indicadores ,Estado ecológico ,Organismos patógenos ,Salud ambiental - Published
- 2015
13. ¿Pueden Mejorar su PAA los Alumnos de Escasos Recursos? Evidencia Experimental
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Javier Núñez E. and Isabel Millán V.
- Subjects
education ,jel:I38 ,Education, multiple-choice test, social mobility ,jel:I21 ,General Economics, Econometrics and Finance ,Multiple-Choice Test ,Social Mobility ,Education - Abstract
Este artículo reporta los resultados de una investigación en el área de la educación en Chile que tuvo por objeto analizar el grado de respuesta del desempeño académico de alumnos de escasos recursos a programas de reforzamiento educacional. La conclusión principal del estudio es que el rendimiento de estos alumnos (medido como puntajes en la Prueba de Aptitud Académica, (PAA) es altamente sensible a programas de entrenamiento y reforzamiento académico, incluso en períodos breves. En un programa diseñado para estos efectos, los alumnos participantes en promedio lograron elevar sus puntajes en la PAA de Matemáticas respecto de sus pares (un grupo de control) en 40 puntos, tras un período de entrenamiento de sólo cuatro meses. Esta cifra aumentaba a 96 puntos en aquellos alumnos con una asistencia superior o igual al 80 por ciento de las clases impartidas. Este importante resultado señala que, con esfuerzo y métodos adecuados, es posible generar avances significativos en materia de educación en períodos breves, a pesar de las deficiencias educacionales y factores socioeconómicos que frecuentemente circundan a esta población estudiantil. Por lo tanto, esta investigación provee evidencia sobre una forma probada de reducir las inequidades en el acceso de alumnos de escasos recursos a la educación superior, además de plantear algunas implicaciones para el diseño de políticas en el área de la educación media y superior This article reports the results of an experiment aiming to investigate the effect of training on the performance of poor students in PAA, a compulsory multiple-choice test required to enroll in university education in Chile. The main conclusion is that poor students´ performance in highly responsive to training, even in a short span of time. The trained students increased their scores in 40 points in only four months relative to a proper control group. This difference raises up to 96 points for those attending over 80 percent of the sessions. This suggests that by means of appropriate effort and training methods, important achievements can be obtained in the short run in poor students´ academic performance and future educational prospects, despite their adverse economic and social background. These findings suggest some policy implications for public policy in the areas of education, and it raises some questions for future research
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- 2002
14. Identificación de indicadores del estado ecológico de zonas litorales de interés ambiental, recreativo y productivo
- Author
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González-Lorenzo, J.G. (José Gustavo), González-Gil, S. (Sonsoles), Jiménez, S. (Sebastián), González, J.F. (José Francisco), Hernández-González, C.L. (Carlos Luis), Martín-Sosa, P. (Pablo), Tello-Antón, M.O. (María Olvido), Rodríguez, J., Boza-Vindel, C. (Cristina), Bartolomé-Baraza, A. (Aurora), González, Y., González, E.L. (Erika Lucía), Armas, D. (Demetrio) de, Santamaría, M.T.G. (María Teresa García), Bethancort, J., Millán, V., and Rodrigo, M.
- Published
- 2014
15. Activité 2: Identification d'indicateurs de l'état écologique de zones littorales d'intérêt environnemental, récréatif et productif
- Author
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Santamaría, M.T.G. (María Teresa García), Bethancort, J., Millán, V., Rodrigo, M., González-Lorenzo, J.G. (José Gustavo), González-Gil, S. (Sonsoles), Jiménez, S. (Sebastián), González, J.F. (José Francisco), Hernández-González, C.L. (Carlos Luis), Martín-Sosa, P. (Pablo), Tello-Antón, M.O. (María Olvido), Rodríguez, E., Boza-Vindel, C. (Cristina), Bartolomé-Baraza, A. (Aurora), González, Y., González, E.L. (Erika Lucía), and Armas, D. (Demetrio) de
- Published
- 2014
16. Identificación de indicadores del estado ecológico de zonas litorales de interés ambiental, recreativo y productivo
- Author
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González-Lorenzo, José Gustavo, González-Gil, Sonsoles, Jiménez, Sebastián, González, José Francisco, Hernández-González, Carlos Luis, Martín-Sosa, Pablo, Tello-Antón, María Olvido, Rodríguez, J., Boza-Vindel, Cristina, Bartolomé-Baraza, Aurora, González, Y., González, Erika Lucía, Armas, Demetrio de, Santamaría, María Teresa García, Bethancort, J., Millán, V., Rodrigo, M., González-Lorenzo, José Gustavo, González-Gil, Sonsoles, Jiménez, Sebastián, González, José Francisco, Hernández-González, Carlos Luis, Martín-Sosa, Pablo, Tello-Antón, María Olvido, Rodríguez, J., Boza-Vindel, Cristina, Bartolomé-Baraza, Aurora, González, Y., González, Erika Lucía, Armas, Demetrio de, Santamaría, María Teresa García, Bethancort, J., Millán, V., and Rodrigo, M.
- Published
- 2014
17. Activité 2: Identification d'indicateurs de l'état écologique de zones littorales d'intérêt environnemental, récréatif et productif
- Author
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Santamaría, María Teresa García, Bethancort, J., Millán, V., Rodrigo, M., González-Lorenzo, José Gustavo, González-Gil, Sonsoles, Jiménez, Sebastián, González, José Francisco, Hernández-González, Carlos Luis, Martín-Sosa, Pablo, Tello-Antón, María Olvido, Rodríguez, E., Boza-Vindel, Cristina, Bartolomé-Baraza, Aurora, González, Y., González, Erika Lucía, Armas, Demetrio de, Santamaría, María Teresa García, Bethancort, J., Millán, V., Rodrigo, M., González-Lorenzo, José Gustavo, González-Gil, Sonsoles, Jiménez, Sebastián, González, José Francisco, Hernández-González, Carlos Luis, Martín-Sosa, Pablo, Tello-Antón, María Olvido, Rodríguez, E., Boza-Vindel, Cristina, Bartolomé-Baraza, Aurora, González, Y., González, Erika Lucía, and Armas, Demetrio de
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- 2014
18. Climatic information of Western Sahel (1535–1793 AD) in original documentary sources
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Millán, V., primary and Rodrigo, F. S., additional
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- 2014
- Full Text
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19. The ATLAS Experiment at the CERN Large Hadron Collider
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Collaboration, The ATLAS, primary, Aad, G, additional, Abat, E, additional, Abdallah, J, additional, Abdelalim, A A, additional, Abdesselam, A, additional, Abdinov, O, additional, Abi, B A, additional, Abolins, M, additional, Abramowicz, H, additional, Acerbi, E, additional, Acharya, B S, additional, Achenbach, R, additional, Ackers, M, additional, Adams, D L, additional, Adamyan, F, additional, Addy, T N, additional, Aderholz, M, additional, Adorisio, C, additional, Adragna, P, additional, Aharrouche, M, additional, Ahlen, S P, additional, Ahles, F, additional, Ahmad, A, additional, Ahmed, H, additional, Aielli, G, additional, Åkesson, P F, additional, Åkesson, T P A, additional, Akimov, A V, additional, Alam, S M, additional, Albert, J, additional, Albrand, S, additional, Aleksa, M, additional, Aleksandrov, I N, additional, Aleppo, M, additional, Alessandria, F, additional, Alexa, C, additional, Alexander, G, additional, Alexopoulos, T, additional, Alimonti, G, additional, Aliyev, M, additional, Allport, P P, additional, Allwood-Spiers, S E, additional, Aloisio, A, additional, Alonso, J, additional, Alves, R, additional, Alviggi, M G, additional, Amako, K, additional, Amaral, P, additional, Amaral, S P, additional, Ambrosini, G, additional, Ambrosio, G, additional, Amelung, C, additional, Ammosov, V V, additional, Amorim, A, additional, Amram, N, additional, Anastopoulos, C, additional, Anderson, B, additional, Anderson, K J, additional, Anderssen, E C, additional, Andreazza, A, additional, Andrei, V, additional, Andricek, L, additional, Andrieux, M-L, additional, Anduaga, X S, additional, Anghinolfi, F, additional, Antonaki, A, additional, Antonelli, M, additional, Antonelli, S, additional, Apsimon, R, additional, Arabidze, G, additional, Aracena, I, additional, Arai, Y, additional, Arce, A T H, additional, Archambault, J P, additional, Arguin, J-F, additional, Arik, E, additional, Arik, M, additional, Arms, K E, additional, Armstrong, S R, additional, Arnaud, M, additional, Arnault, C, additional, Artamonov, A, additional, Asai, S, additional, Ask, S, additional, Åsman, B, additional, Asner, D, additional, Asquith, L, additional, Assamagan, K, additional, Astbury, A, additional, Athar, B, additional, Atkinson, T, additional, Aubert, B, additional, Auerbach, B, additional, Auge, E, additional, Augsten, K, additional, Aulchenko, V M, additional, Austin, N, additional, Avolio, G, additional, Avramidou, R, additional, Axen, A, additional, Ay, C, additional, Azuelos, G, additional, Baccaglioni, G, additional, Bacci, C, additional, Bachacou, H, additional, Bachas, K, additional, Bachy, G, additional, Badescu, E, additional, Bagnaia, P, additional, Bailey, D C, additional, Baines, J T, additional, Baker, O K, additional, Ballester, F, additional, Pedrosa, F Baltasar Dos Santos, additional, Banas, E, additional, Banfi, D, additional, Bangert, A, additional, Bansal, V, additional, Baranov, S P, additional, Baranov, S, additional, Barashkou, A, additional, Barberio, E L, additional, Barberis, D, additional, Barbier, G, additional, Barclay, P, additional, Bardin, D Y, additional, Bargassa, P, additional, Barillari, T, additional, Barisonzi, M, additional, Barnett, B M, additional, Barnett, R M, additional, Baron, S, additional, Baroncelli, A, additional, Barone, M, additional, Barr, A J, additional, Barreiro, F, additional, Costa, J Barreiro Guimarães da, additional, Barrillon, P, additional, Poy, A Barriuso, additional, Barros, N, additional, Bartheld, V, additional, Bartko, H, additional, Bartoldus, R, additional, Basiladze, S, additional, Bastos, J, additional, Batchelor, L E, additional, Bates, R L, additional, Batley, J R, additional, Batraneanu, S, additional, Battistin, M, additional, Battistoni, G, additional, Batusov, V, additional, Bauer, F, additional, Bauss, B, additional, Baynham, D E, additional, Bazalova, M, additional, Bazan, A, additional, Beauchemin, P H, additional, Beaugiraud, B, additional, Beccherle, R B, additional, Beck, G A, additional, Beck, H P, additional, Becks, K H, additional, Bedajanek, I, additional, Beddall, A J, additional, Beddall, A, additional, Bednár, P, additional, Bednyakov, V A, additional, Bee, C, additional, Harpaz, S Behar, additional, Belanger, G A N, additional, Belanger-Champagne, C, additional, Belhorma, B, additional, Bell, P J, additional, Bell, W H, additional, Bella, G, additional, Bellachia, F, additional, Bellagamba, L, additional, Bellina, F, additional, Bellomo, G, additional, Bellomo, M, additional, Beltramello, O, additional, Belymam, A, additional, Ami, S Ben, additional, Moshe, M Ben, additional, Benary, O, additional, Benchekroun, D, additional, Benchouk, C, additional, Bendel, M, additional, Benedict, B H, additional, Benekos, N, additional, Benes, J, additional, Benhammou, Y, additional, Benincasa, G P, additional, Benjamin, D P, additional, Bensinger, J R, additional, Benslama, K, additional, Bentvelsen, S, additional, Beretta, M, additional, Berge, D, additional, Bergeaas, E, additional, Berger, N, additional, Berghaus, F, additional, Berglund, S, additional, Bergsma, F, additional, Beringer, J, additional, Bernabéu, J, additional, Bernardet, K, additional, Berriaud, C, additional, Berry, T, additional, Bertelsen, H, additional, Bertin, A, additional, Bertinelli, F, additional, Bertolucci, S, additional, Besson, N, additional, Beteille, A, additional, Bethke, S, additional, Bialas, W, additional, Bianchi, R M, additional, Bianco, M, additional, Biebel, O, additional, Bieri, M, additional, Biglietti, M, additional, Bilokon, H, additional, Binder, M, additional, Binet, S, additional, Bingefors, N, additional, Bingul, A, additional, Bini, C, additional, Biscarat, C, additional, Bischof, R, additional, Bischofberger, M, additional, Bitadze, A, additional, Bizzell, J P, additional, Black, K M, additional, Blair, R E, additional, Blaising, J J, additional, Blanch, O, additional, Blanchot, G, additional, Blocker, C, additional, Blocki, J, additional, Blondel, A, additional, Blum, W, additional, Blumenschein, U, additional, Boaretto, C, additional, Bobbink, G J, additional, Bocci, A, additional, Bocian, D, additional, Bock, R, additional, Boehm, M, additional, Boek, J, additional, Bogaerts, J A, additional, Bogouch, A, additional, Bohm, C, additional, Bohm, J, additional, Boisvert, V, additional, Bold, T, additional, Boldea, V, additional, Bondarenko, V G, additional, Bonino, R, additional, Bonis, J, additional, Bonivento, W, additional, Bonneau, P, additional, Boonekamp, M, additional, Boorman, G, additional, Boosten, M, additional, Booth, C N, additional, Booth, P S L, additional, Booth, P, additional, Booth, J R A, additional, Borer, K, additional, Borisov, A, additional, Borjanovic, I, additional, Bos, K, additional, Boscherini, D, additional, Bosi, F, additional, Bosman, M, additional, Bosteels, M, additional, Botchev, B, additional, Boterenbrood, H, additional, Botterill, D, additional, Boudreau, J, additional, Bouhova-Thacker, E V, additional, Boulahouache, C, additional, Bourdarios, C, additional, Boutemeur, M, additional, Bouzakis, K, additional, Boyd, G R, additional, Boyd, J, additional, Boyer, B H, additional, Boyko, I R, additional, Bozhko, N I, additional, Braccini, S, additional, Braem, A, additional, Branchini, P, additional, Brandenburg, G W, additional, Brandt, A, additional, Brandt, O, additional, Bratzler, U, additional, Braun, H M, additional, Bravo, S, additional, Brawn, I P, additional, Brelier, B, additional, Bremer, J, additional, Brenner, R, additional, Bressler, S, additional, Breton, D, additional, Brett, N D, additional, Breugnon, P, additional, Bright-Thomas, P G, additional, Brochu, F M, additional, Brock, I, additional, Brock, R, additional, Brodbeck, T J, additional, Brodet, E, additional, Broggi, F, additional, Broklova, Z, additional, Bromberg, C, additional, Brooijmans, G, additional, Brouwer, G, additional, Broz, J, additional, Brubaker, E, additional, Renstrom, P A Bruckman de, additional, Bruncko, D, additional, Bruni, A, additional, Bruni, G, additional, Bruschi, M, additional, Buanes, T, additional, Buchanan, N J, additional, Buchholz, P, additional, Budagov, I A, additional, Büscher, V, additional, Bugge, L, additional, Buira-Clark, D, additional, Buis, E J, additional, Bujor, F, additional, Buran, T, additional, Burckhart, H, additional, Burckhart-Chromek, D, additional, Burdin, S, additional, Burns, R, additional, Busato, E, additional, Buskop, J J F, additional, Buszello, K P, additional, Butin, F, additional, Butler, J M, additional, Buttar, C M, additional, Butterworth, J, additional, Butterworth, J M, additional, Byatt, T, additional, Urbán, S Cabrera, additional, Casas, E Cabruja, additional, Caccia, M, additional, Caforio, D, additional, Cakir, O, additional, Calafiura, P, additional, Calderini, G, additional, Terol, D Calderón, additional, Callahan, J, additional, Caloba, L P, additional, Caloi, R, additional, Calvet, D, additional, Camard, A, additional, Camarena, F, additional, Camarri, P, additional, Cambiaghi, M, additional, Cameron, D, additional, Cammin, J, additional, Segura, F Campabadal, additional, Campana, S, additional, Canale, V, additional, Cantero, J, additional, Garrido, M D M Capeans, additional, Caprini, I, additional, Caprini, M, additional, Caprio, M, additional, Caracinha, D, additional, Caramarcu, C, additional, Carcagno, Y, additional, Cardarelli, R, additional, Cardeira, C, additional, Sas, L Cardiel, additional, Cardini, A, additional, Carli, T, additional, Carlino, G, additional, Carminati, L, additional, Caron, B, additional, Caron, S, additional, Carpentieri, C, additional, Carr, F S, additional, Carter, A A, additional, Carter, J R, additional, Carvalho, J, additional, Casadei, D, additional, Casado, M P, additional, Cascella, M, additional, Caso, C, additional, Castelo, J, additional, Gimenez, V Castillo, additional, Castro, N, additional, Castrovillari, F, additional, Cataldi, G, additional, Cataneo, F, additional, Catinaccio, A, additional, Catmore, J R, additional, Cattai, A, additional, Caughron, S, additional, Cauz, D, additional, Cavallari, A, additional, Cavalleri, P, additional, Cavalli, D, additional, Cavalli-Sforza, M, additional, Cavasinni, V, additional, Ceradini, F, additional, Cerna, C, additional, Cernoch, C, additional, Cerqueira, A S, additional, Cerri, A, additional, Cerutti, F, additional, Cervetto, M, additional, Cetin, S A, additional, Cevenini, F, additional, Chalifour, M, additional, llatas, M Chamizo, additional, Chan, A, additional, Chapman, J W, additional, Charlton, D G, additional, Charron, S, additional, Chekulaev, S V, additional, Chelkov, G A, additional, Chen, H, additional, Chen, L, additional, Chen, T, additional, Chen, X, additional, Cheng, S, additional, Cheng, T L, additional, Cheplakov, A, additional, Chepurnov, V F, additional, Moursli, R Cherkaoui El, additional, Chesneanu, D, additional, Cheu, E, additional, Chevalier, L, additional, Chevalley, J L, additional, Chevallier, F, additional, Chiarella, V, additional, Chiefari, G, additional, Chikovani, L, additional, Chilingarov, A, additional, Chiodini, G, additional, Chouridou, S, additional, Chren, D, additional, Christiansen, T, additional, Christidi, I A, additional, Christov, A, additional, Chu, M L, additional, Chudoba, J, additional, Chuguev, A G, additional, Ciapetti, G, additional, Cicalini, E, additional, Ciftci, A K, additional, Cindro, V, additional, Ciobotaru, M D, additional, Ciocio, A, additional, Cirilli, M, additional, Citterio, M, additional, Ciubancan, M, additional, Civera, J V, additional, Clark, A, additional, Cleland, W, additional, Clemens, J C, additional, Clement, B C, additional, Clément, C, additional, Clements, D, additional, Clifft, R W, additional, Cobal, M, additional, Coccaro, A, additional, Cochran, J, additional, Coco, R, additional, Coe, P, additional, Coelli, S, additional, Cogneras, E, additional, Cojocaru, C D, additional, Colas, J, additional, Colijn, A P, additional, Collard, C, additional, Collins-Tooth, C, additional, Collot, J, additional, Coluccia, R, additional, Comune, G, additional, Muiño, P Conde, additional, Coniavitis, E, additional, Consonni, M, additional, Constantinescu, S, additional, Conta, C, additional, Conventi, F A, additional, Cook, J, additional, Cooke, M, additional, Cooper-Smith, N J, additional, Cornelissen, T, additional, Corradi, M, additional, Correard, S, additional, Corso-Radu, A, additional, Coss, J, additional, Costa, G, additional, Costa, M J, additional, Costanzo, D, additional, Costin, T, additional, Torres, R Coura, additional, Courneyea, L, additional, Couyoumtzelis, C, additional, Cowan, G, additional, Cox, B E, additional, Cox, J, additional, Cragg, D A, additional, Cranmer, K, additional, Cranshaw, J, additional, Cristinziani, M, additional, Crosetti, G, additional, Almenar, C Cuenca, additional, Cuneo, S, additional, Cunha, A, additional, Curatolo, M, additional, Curtis, C J, additional, Cwetanski, P, additional, Czyczula, Z, additional, D'Auria, S, additional, D'Onofrio, M, additional, Mello, A Da Rocha Gesualdi, additional, Silva, P V M Da, additional, Silva, R Da, additional, Dabrowski, W, additional, Dael, A, additional, Dahlhoff, A, additional, Dai, T, additional, Dallapiccola, C, additional, Dallison, S J, additional, Dalmau, J, additional, Daly, C H, additional, Dam, M, additional, Damazio, D, additional, Dameri, M, additional, Danielsen, K M, additional, Danielsson, H O, additional, Dankers, R, additional, Dannheim, D, additional, Darbo, G, additional, Dargent, P, additional, Daum, C, additional, Dauvergne, J P, additional, David, M, additional, Davidek, T, additional, Davidson, N, additional, Davidson, R, additional, Dawson, I, additional, Dawson, J W, additional, Daya, R K, additional, De, K, additional, Asmundis, R de, additional, Boer, R de, additional, Castro, S De, additional, Groot, N De, additional, Jong, P de, additional, Broise, X de La, additional, Cruz-Burelo, E De La, additional, Taille, C De La, additional, Lotto, B De, additional, Branco, M De Oliveira, additional, Pedis, D De, additional, Saintignon, P de, additional, Salvo, A De, additional, Sanctis, U De, additional, Santo, A De, additional, Regie, J B De Vivie De, additional, Zorzi, G De, additional, Dean, S, additional, Dedes, G, additional, Dedovich, D V, additional, Defay, P O, additional, Degele, R, additional, Dehchar, M, additional, Deile, M, additional, Papa, C Del, additional, Peso, J Del, additional, Prete, T Del, additional, Delagnes, E, additional, Delebecque, P, additional, Dell'Acqua, A, additional, Pietra, M Della, additional, Volpe, D della, additional, Delmastro, M, additional, Delpierre, P, additional, Delruelle, N, additional, Delsart, P A, additional, Silberberg, C Deluca, additional, Demers, S, additional, Demichev, M, additional, Demierre, P, additional, Demirköz, B, additional, Deng, W, additional, Denisov, S P, additional, Dennis, C, additional, Densham, C J, additional, Dentan, M, additional, Derkaoui, J E, additional, Derue, F, additional, Dervan, P, additional, Desch, K K, additional, Dewhurst, A, additional, Ciaccio, A Di, additional, Ciaccio, L Di, additional, Domenico, A Di, additional, Girolamo, A Di, additional, Girolamo, B Di, additional, Luise, S Di, additional, Mattia, A Di, additional, Simone, A Di, additional, Gomez, M M Diaz, additional, Diehl, E B, additional, Dietl, H, additional, Dietrich, J, additional, Dietsche, W, additional, Diglio, S, additional, Dima, M, additional, Dindar, K, additional, Dinkespiler, B, additional, Dionisi, C, additional, Dipanjan, R, additional, Dita, P, additional, Dita, S, additional, Dittus, F, additional, Dixon, S D, additional, Djama, F, additional, Djilkibaev, R, additional, Djobava, T, additional, Vale, M A B do, additional, Dobbs, M, additional, Dobinson, R, additional, Dobos, D, additional, Dobson, E, additional, Dobson, M, additional, Dodd, J, additional, Dogan, O B, additional, Doherty, T, additional, Doi, Y, additional, Dolejsi, J, additional, Dolenc, I, additional, Dolezal, Z, additional, Dolgoshein, B A, additional, Domingo, E, additional, Donega, M, additional, Dopke, J, additional, Dorfan, D E, additional, Dorholt, O, additional, Doria, A, additional, Anjos, A Dos, additional, Dosil, M, additional, Dotti, A, additional, Dova, M T, additional, Dowell, J D, additional, Doyle, A T, additional, Drake, G, additional, Drakoulakos, D, additional, Drasal, Z, additional, Drees, J, additional, Dressnandt, N, additional, Drevermann, H, additional, Driouichi, C, additional, Dris, M, additional, Drohan, J G, additional, Dubbert, J, additional, Dubbs, T, additional, Duchovni, E, additional, Duckeck, G, additional, Dudarev, A, additional, Dührssen, M, additional, Dür, H, additional, Duerdoth, I P, additional, Duffin, S, additional, Duflot, L, additional, Dufour, M-A, additional, Dayot, N Dumont, additional, Yildiz, H Duran, additional, Durand, D, additional, Dushkin, A, additional, Duxfield, R, additional, Dwuznik, M, additional, Dydak, F, additional, Dzahini, D, additional, Cornell, S Díez, additional, Düren, M, additional, Ebenstein, W L, additional, Eckert, S, additional, Eckweiler, S, additional, Eerola, P, additional, Efthymiopoulos, I, additional, Egede, U, additional, Egorov, K, additional, Ehrenfeld, W, additional, Eifert, T, additional, Eigen, G, additional, Einsweiler, K, additional, Eisenhandler, E, additional, Ekelof, T, additional, Eklund, L M, additional, Kacimi, M El, additional, Ellert, M, additional, Elles, S, additional, Ellis, N, additional, Elmsheuser, J, additional, Elsing, M, additional, Ely, R, additional, Emeliyanov, D, additional, Engelmann, R, additional, Engström, M, additional, Ennes, P, additional, Epp, B, additional, Eppig, A, additional, Epshteyn, V S, additional, Ereditato, A, additional, Eremin, V, additional, Eriksson, D, additional, Ermoline, I, additional, Ernwein, J, additional, Errede, D, additional, Errede, S, additional, Escalier, M, additional, Escobar, C, additional, Curull, X Espinal, additional, Esposito, B, additional, Esteves, F, additional, Etienne, F, additional, Etienvre, A I, additional, Etzion, E, additional, Evans, H, additional, Evdokimov, V N, additional, Evtoukhovitch, P, additional, Eyring, A, additional, Fabbri, L, additional, Fabjan, C W, additional, Fabre, C, additional, Faccioli, P, additional, Facius, K, additional, Fadeyev, V, additional, Fakhrutdinov, R M, additional, Falciano, S, additional, Falleau, I, additional, Falou, A C, additional, Fang, Y, additional, Fanti, M, additional, Farbin, A, additional, Farilla, A, additional, Farrell, J, additional, Farthouat, P, additional, Fasching, D, additional, Fassi, F, additional, Fassnacht, P, additional, Fassouliotis, D, additional, Fawzi, F, additional, Fayard, L, additional, Fayette, F, additional, Febbraro, R, additional, Fedin, O L, additional, Fedorko, I, additional, Feld, L, additional, Feldman, G, additional, Feligioni, L, additional, Feng, C, additional, Feng, E J, additional, Fent, J, additional, Fenyuk, A B, additional, Ferencei, J, additional, Ferguson, D, additional, Ferland, J, additional, Fernando, W, additional, Ferrag, S, additional, Ferrari, A, additional, Ferrari, P, additional, Ferrari, R, additional, Ferrer, A, additional, Ferrer, M L, additional, Ferrere, D, additional, Ferretti, C, additional, Ferro, F, additional, Fiascaris, M, additional, Fichet, S, additional, Fiedler, F, additional, Filimonov, V, additional, Filipčič, A, additional, Filippas, A, additional, Filthaut, F, additional, Fincke-Keeler, M, additional, Finocchiaro, G, additional, Fiorini, L, additional, Firan, A, additional, Fischer, P, additional, Fisher, M J, additional, Fisher, S M, additional, Flaminio, V, additional, Flammer, J, additional, Flechl, M, additional, Fleck, I, additional, Flegel, W, additional, Fleischmann, P, additional, Fleischmann, S, additional, Corral, C M Fleta, additional, Fleuret, F, additional, Flick, T, additional, Flix, J, additional, Castillo, L R Flores, additional, Flowerdew, M J, additional, Föhlisch, F, additional, Fokitis, M, additional, Martin, T M Fonseca, additional, Fopma, J, additional, Forbush, D A, additional, Formica, A, additional, Foster, J M, additional, Fournier, D, additional, Foussat, A, additional, Fowler, A J, additional, Fox, H, additional, Francavilla, P, additional, Francis, D, additional, Franz, S, additional, Fraser, J T, additional, Fraternali, M, additional, Fratianni, S, additional, Freestone, J, additional, French, R S, additional, Fritsch, K, additional, Froidevaux, D, additional, Frost, J A, additional, Fukunaga, C, additional, Fulachier, J, additional, Torregrosa, E Fullana, additional, Fuster, J, additional, Gabaldon, C, additional, Gadomski, S, additional, Gagliardi, G, additional, Gagnon, P, additional, Gallas, E J, additional, Gallas, M V, additional, Gallop, B J, additional, Gan, K K, additional, Gannaway, F C, additional, Gao, Y S, additional, Gapienko, V A, additional, Gaponenko, A, additional, Garciá, C, additional, Garcia-Sciveres, M, additional, Navarro, J E Garcìa, additional, Garde, V, additional, Gardner, R W, additional, Garelli, N, additional, Garitaonandia, H, additional, Garonne, V G, additional, Garvey, J, additional, Gatti, C, additional, Gaudio, G, additional, Gaumer, O, additional, Gautard, V, additional, Gauzzi, P, additional, Gavrilenko, I L, additional, Gay, C, additional, Gayde, J-C, additional, Gazis, E N, additional, Gazo, E, additional, Gee, C N P, additional, Geich-Gimbel, C, additional, Gellerstedt, K, additional, Gemme, C, additional, Genest, M H, additional, Gentile, S, additional, George, M A, additional, George, S, additional, Gerlach, P, additional, Gernizky, Y, additional, Geweniger, C, additional, Ghazlane, H, additional, Ghete, V M, additional, Ghez, P, additional, Ghodbane, N, additional, Giacobbe, B, additional, Giagu, S, additional, Giakoumopoulou, V, additional, Giangiobbe, V, additional, Gianotti, F, additional, Gibbard, B, additional, Gibson, A, additional, Gibson, M D, additional, Gibson, S M, additional, Gieraltowski, G F, additional, Botella, I Gil, additional, Gilbert, L M, additional, Gilchriese, M, additional, Gildemeister, O, additional, Gilewsky, V, additional, Gillman, A R, additional, Gingrich, D M, additional, Ginzburg, J, additional, Giokaris, N, additional, Giordani, M P, additional, Girard, C G, additional, Giraud, P F, additional, Girtler, P, additional, Giugni, D, additional, Giusti, P, additional, Gjelsten, B K, additional, Glasman, C, additional, Glazov, A, additional, Glitza, K W, additional, Glonti, G L, additional, Gnanvo, K G, additional, Godlewski, J, additional, Göpfert, T, additional, Gössling, C, additional, Göttfert, T, additional, Goldfarb, S, additional, Goldin, D, additional, Goldschmidt, N, additional, Golling, T, additional, Gollub, N P, additional, Golonka, P J, additional, Golovnia, S N, additional, Gomes, A, additional, Gomes, J, additional, Gonçalo, R, additional, Gongadze, A, additional, Gonidec, A, additional, Gonzalez, S, additional, Hoz, S González de la, additional, Millán, V González, additional, Silva, M L Gonzalez, additional, Gonzalez-Pineiro, B, additional, González-Sevilla, S, additional, Goodrick, M J, additional, Goodson, J J, additional, Goossens, L, additional, Gorbounov, P A, additional, Gordeev, A, additional, Gordon, H, additional, Gorelov, I, additional, Gorfine, G, additional, Gorini, B, additional, Gorini, E, additional, Gorišek, A, additional, Gornicki, E, additional, Gorokhov, S A, additional, Gorski, B T, additional, Goryachev, S V, additional, Goryachev, V N, additional, Gosselink, M, additional, Gostkin, M I, additional, Gouanère, M, additional, Eschrich, I Gough, additional, Goujdami, D, additional, Goulette, M, additional, Gousakov, I, 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additional, Gschwendtner, E M, additional, Guarino, V J, additional, Guicheney, C J, additional, Guilhem, G, additional, Guillemin, T, additional, Gunther, J, additional, Guo, B, additional, Gupta, A, additional, Gurriana, L, additional, Gushchin, V N, additional, Gutierrez, P, additional, Guy, L, additional, Guyot, C, additional, Gwenlan, C, additional, Gwilliam, C B, additional, Haas, A, additional, Haas, S, additional, Haber, C, additional, Haboubi, G, additional, Hackenburg, R, additional, Hadash, E, additional, Hadavand, H K, additional, Haeberli, C, additional, Härtel, R, additional, Haggerty, R, additional, Hahn, F, additional, Haider, S, additional, Hajduk, Z, additional, Hakimi, M, additional, Hakobyan, H, additional, Haller, J, additional, Hallewell, G D, additional, Hallgren, B, additional, Hamacher, K, additional, Hamilton, A, additional, Han, H, additional, Han, L, additional, Hanagaki, K, additional, Hance, M, additional, Hanke, P, additional, Hansen, C J, additional, Hansen, F H, additional, Hansen, J R, additional, Hansen, J B, additional, Hansen, J D, additional, Hansen, P H, additional, Hansl-Kozanecka, T, additional, Hanson, G, additional, Hansson, P, additional, Hara, K, additional, Harder, S, additional, Harel, A, additional, Harenberg, T, additional, Harper, R, additional, Hart, J C, additional, Hart, R G G, additional, Hartjes, F, additional, Hartman, N, additional, Haruyama, T, additional, Harvey, A, additional, Hasegawa, Y, additional, Hashemi, K, additional, Hassani, S, additional, Hatch, M, additional, Hatley, R W, additional, Haubold, T G, additional, Hauff, D, additional, Haug, F, additional, Haug, S, additional, Hauschild, M, additional, Hauser, R, additional, Hauviller, C, additional, Havranek, M, additional, Hawes, B M, additional, Hawkings, R J, additional, Hawkins, D, additional, Hayler, T, additional, Hayward, H S, additional, Haywood, S J, additional, Hazen, E, additional, He, M, additional, He, Y P, additional, Head, S J, additional, Hedberg, V, additional, Heelan, L, additional, Heinemann, F E W, additional, Heldmann, M, additional, Hellman, S, additional, Helsens, C, additional, Henderson, R C W, additional, Hendriks, P J, additional, Correia, A M Henriques, additional, Henrot-Versille, S, additional, Henry-Couannier, F, additional, Henß, T, additional, Herten, G, additional, Hertenberger, R, additional, Hervas, L, additional, Hess, M, additional, Hessey, N P, additional, Hicheur, A, additional, Hidvegi, A, additional, Higón-Rodriguez, E, additional, Hill, D, additional, Hill, J, additional, Hill, J C, additional, Hill, N, additional, Hillier, S J, additional, Hinchliffe, I, additional, Hindson, D, additional, Hinkelbein, C, additional, Hodges, T A, additional, Hodgkinson, M C, additional, Hodgson, P, additional, Hoecker, A, additional, Hoeferkamp, M R, additional, Hoffman, J, additional, Hoffmann, A E, additional, Hoffmann, D, additional, Hoffmann, H F, additional, Holder, M, additional, Hollins, T I, additional, Hollyman, G, additional, Holmes, A, additional, Holmgren, S O, additional, Holt, R, additional, Holtom, E, additional, Holy, T, additional, Homer, R J, additional, Homma, Y, additional, Homola, P, additional, Honerbach, W, additional, Honma, A, additional, Hooton, I, additional, Horazdovsky, T, additional, Horn, C, additional, Horvat, S, additional, Hostachy, J-Y, additional, Hott, T, additional, Hou, S, additional, Houlden, M A, additional, Hoummada, A, additional, Hover, J, additional, Howell, D F, additional, Hrivnac, J, additional, Hruska, I, additional, Hryn'ova, T, additional, Huang, G S, additional, Hubacek, Z, additional, Hubaut, F, additional, Huegging, F, additional, Huffman, B T, additional, Hughes, E, additional, Hughes, G, additional, Hughes-Jones, R E, additional, Hulsbergen, W, additional, Hurst, P, additional, Hurwitz, M, additional, Huse, T, additional, Huseynov, N, additional, Huston, J, additional, Huth, J, additional, Iacobucci, G, additional, 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additional, Nikolic-Audit, I, additional, Nikolopoulos, K, additional, Nilsen, H, additional, Nilsson, B S, additional, Nilsson, P, additional, Nisati, A, additional, Nisius, R, additional, Nodulman, L J, additional, Nomachi, M, additional, Nomoto, H, additional, Noppe, J-M, additional, Nordberg, M, additional, Francisco, O Norniella, additional, Norton, P R, additional, Novakova, J, additional, Nowak, M, additional, Nozaki, M, additional, Nunes, R, additional, Hanninger, G Nunes, additional, Nunnemann, T, additional, Nyman, T, additional, O'Connor, P, additional, O'Neale, S W, additional, O'Neil, D C, additional, O'Neill, M, additional, O'Shea, V, additional, Oakham, F G, additional, Oberlack, H, additional, Obermaier, M, additional, Oberson, P, additional, Ochi, A, additional, Ockenfels, W, additional, Odaka, S, additional, Odenthal, I, additional, Odino, G A, additional, Ogren, H, additional, Oh, S H, additional, Ohshima, T, additional, Ohshita, H, additional, Okawa, H, additional, 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- 2008
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20. Climatic information of Western Sahel (1535-1793 AD) in original documentary sources.
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Millán, V. and Rodrigo, F. S.
- Abstract
The Sahel is the semi-arid transition zone between arid Sahara and humid tropical Africa, extending approximately 10-20° N from Mauritania in the West to Sudan in the East. The African continent, one of the most vulnerable regions to climate change, is subject to frequent droughts and famine. One climate challenge research is to iso-late those aspects of climate variability that are natural from those that are related to human influences. Therefore, the study of climatic conditions before mid-19th cen-tury, when anthropogenic influence was of minor importance, is very interesting. In this work the frequency of extreme events, such as droughts and floods, in Western Sa-hel from the 16th to 18th centuries is investigated using documentary data. Original manuscripts with historical chronicles from Walata and Nema (Mauritania), Timbuktu and Arawan (Mali), and Agadez (Niger) have been analyzed. Information on droughts, intense rainfall, storms and floods, as well as socioeconomic aspects (famines, pests, scarcity, prosperity) has been codified in an ordinal scale ranging from -2 (drought and famines) to +2 (floods) to obtain a numerical index of the annual rainfall in the region. Results show wet conditions in the 17th century, as well as dry conditions in the 18th century (interrupted by a short wet period in the 1730s decade). [ABSTRACT FROM AUTHOR]
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- 2014
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21. A computer architecture to use AIBO robots to teach Artificial Intelligence | Arquitectura para Utilizar Robots AIBO en la Enseñanza de la Inteligencia Artificial
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Miguel Arevalillo-Herráez, Moreno-Picot, S., and Cavero-Millán, V.
22. Adaptive immunity of type VI CRISPR-Cas systems associated with reverse transcriptase-Cas1 fusion proteins.
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Molina-Sánchez MD, Martínez-Abarca F, Millán V, Mestre MR, Stehantsev P, Stetsenko A, Guskov A, and Toro N
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- Bacterial Proteins genetics, Bacterial Proteins metabolism, Gastrointestinal Microbiome genetics, Gastrointestinal Microbiome immunology, Clustered Regularly Interspaced Short Palindromic Repeats genetics, Bacteria genetics, Bacteria immunology, RNA Interference, CRISPR-Cas Systems, RNA-Directed DNA Polymerase metabolism, RNA-Directed DNA Polymerase genetics, CRISPR-Associated Proteins metabolism, CRISPR-Associated Proteins genetics, Adaptive Immunity genetics
- Abstract
Cas13-containing type VI CRISPR-Cas systems specifically target RNA; however, the mechanism of spacer acquisition remains unclear. We have previously reported the association of reverse transcriptase-Cas1 (RT-Cas1) fusion proteins with certain types of VI-A systems. Here, we show that RT-Cas1 fusion proteins are also recruited by type VI-B systems in bacteria from gut microbiomes, constituting a VI-B1 variant system that includes a CorA-encoding locus in addition to the CRISPR array and the RT-Cas1/Cas2 adaptation module. We found that type VI RT-CRISPR systems were functional for spacer acquisition, CRISPR array processing and interference activity, demonstrating that adaptive immunity mediated by these systems can function independently of other in trans systems. We provide evidence that the RT associated with these systems enables spacer acquisition from RNA molecules. We also found that CorA encoded by type VI-B1 RT-associated systems can transport divalent metal ions and downregulate Cas13b-mediated RNA interference. These findings highlight the importance of RTs in RNA-targeting CRISPR-Cas systems, potentially enabling the integration of RNA-derived spacers into CRISPR arrays as a mechanism against RNA-based invaders in specific environments., (© The Author(s) 2024. Published by Oxford University Press on behalf of Nucleic Acids Research.)
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- 2024
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23. A double-negative feedback loop between NtrBC and a small RNA rewires nitrogen metabolism in legume symbionts.
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García-Tomsig NI, García-Rodriguez FM, Guedes-García SK, Millán V, Becker A, Robledo M, and Jiménez-Zurdo JI
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- Bacterial Proteins genetics, Bacterial Proteins metabolism, Nitrogen Fixation genetics, Medicago sativa microbiology, Medicago sativa metabolism, Medicago sativa genetics, RNA, Bacterial genetics, RNA, Bacterial metabolism, Feedback, Physiological, Root Nodules, Plant microbiology, Root Nodules, Plant metabolism, RNA, Small Untranslated genetics, RNA, Small Untranslated metabolism, Sinorhizobium meliloti genetics, Sinorhizobium meliloti metabolism, Symbiosis, Nitrogen metabolism, Gene Expression Regulation, Bacterial
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Importance: Root nodule endosymbioses between diazotrophic rhizobia and legumes provide the largest input of combined N to the biosphere, thus representing an alternative to harmful chemical fertilizers for sustainable crop production. Rhizobia have evolved intricate strategies to coordinate N assimilation for their own benefit with N
2 fixation to sustain plant growth. The rhizobial N status is transduced by the NtrBC two-component system, the seemingly ubiquitous form of N signal transduction in Proteobacteria. Here, we show that the regulatory sRNA NfeR1 (nodule formation efficiency RNA) of the alfalfa symbiont Sinorhizobium meliloti is transcribed from a complex promoter repressed by NtrC in a N-dependent manner and feedback silences ntrBC by complementary base-pairing. These findings unveil a more prominent role of NtrC as a transcriptional repressor than hitherto anticipated and a novel RNA-based mechanism for NtrBC regulation. The NtrBC-NfeR1 double-negative feedback loop accurately rewires symbiotic S. meliloti N metabolism and is likely conserved in α-rhizobia., Competing Interests: The authors declare no conflict of interest.- Published
- 2023
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24. [Clinical characteristics and prognosis of Staphylococcus aureus bacteremia].
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García Fenoll R, Espinosa Pérez M, Mormeneo Bayo S, Frutos Millán V, Martínez Jiménez MC, Martínez Álvarez RM, Palacián Ruiz MP, Villuendas Usón MC, and Ramos Paesa C
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- Humans, Aged, Staphylococcus aureus, Retrospective Studies, Risk Factors, Prognosis, Staphylococcal Infections drug therapy, Bacteremia complications
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Objective: Staphylococcus aureus bacteremia patients characteristics at a tertiary hospital are described, and complications, mortality and associated factors are analyzed., Methods: Data from patients with S. aureus bacteremia admitted between March 2020 and February2021 at Miguel Servet university hospital in Zaragoza were retrospectively analyzed., Results: Results showed a 14 days mortality of 24.2% and an 30 days mortality of 40%. Overall survival decreased with complications appearance [HR 3.1 (1.2-8.05)] and age over 65 years [HR 3.1 (1.4-6.6)]. The adjusted analysis showed correlation between a higher mortality at 14 and 30 days with age over 65 years [OR 6.3 (1.7-23.1)], sepsis presence [OR 19.3 (5.4-68.7)] and number of positive (+) blood cultures ≥3 [OR 5.4 (0.8-34.1)]. Mortality at 14 days was associated with sepsis presence [OR 58.2 (5.7-592.9)], number of positive (+) blood cultures ≥3 [OR 14.1 (1.1-173.7)] and an older age [OR 1.1 (1.03-1.1)]. Analyzing time to positive blood cultures ≤12 hours and number of positive blood cultures ≥ 3 at the same time, frequency of sepsis increased [30 patients (66.6%) vs 15 patients (33.3%); OR 3.4 (IC95% 1.5-8)]., Conclusions: High 14- and 30-days mortality were found, as well as a worse evolution in older age patients, with sepsis presence, and with greater number of positive blood cultures and times to positive blood cultures ≤12 h., (©The Author 2022. Published by Sociedad Española de Quimioterapia. This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)(https://creativecommons.org/licenses/by-nc/4.0/).)
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- 2022
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25. [Impact of Staphylococcus aureus bacteremia in COVID-19 patients].
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Espinosa Perez M, García Fenoll R, Mormeneo Bayo S, Martínez Álvarez RM, Frutos Millán V, Villuendas Usón MC, Palacián Ruiz MP, Arbonés Mainar JM, Martínez Jiménez MC, and Ramos Paesa C
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- Adult, Dexamethasone, Escherichia coli, Humans, SARS-CoV-2, Staphylococcus aureus, Bacteremia complications, Bacteremia epidemiology, COVID-19 complications, Staphylococcal Infections drug therapy, Staphylococcal Infections epidemiology
- Abstract
Objective: The disease caused by SARS-CoV-2 (COVID-19) has been a challenge for healthcare professionals since its appearance. Staphylococcus aureus has been described as one of the main pathogens causing bacterial infections in viral pandemics. However, co- infection with S. aureus causing bacteremia in patients with COVID-19 has yet to be well studied., Methods: We performed a e study of S. aureus bacteremia (SAB) at Hospital Miguel Servet (Zaragoza) from March 2020 to February 2021. The clinical characteristics, mortality and risk factors of adults hospitalized patients with BSA associated COVID-19 compared to patients without COVID-19., Results: A total of 95 patients with SAB were identified. 27.3% were positive for SARS-CoV-2. SAB represented 9.9% of bacteremia, being the second agent in frequency after E. coli. Nosocomial bacteremia was more frequent in the group of COVID-19 patients. The most frequent source of BSA in these patients was the respiratory source (26.9% vs 0%; P<0.001) followed by the skin (15.5% vs 15.9%; P=1). The development of sepsis was more frequent in COVID-19 patients (61,5% vs 7,8%; P=0,336) and among them, who received dexamethasone at doses > 6 mg/day (62.5% vs. 37.5%, P<0.05)., Conclusions: Our data suggest that BSA has a negative impact on the evolution of patients with COVID-19. However, further and preferably prospective studies are required to obtain solid data on the impact of BSA on coronavirus patients., (©The Author 2022. Published by Sociedad Española de Quimioterapia. This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International (CC BY-NC 4.0)(https://creativecommons.org/licenses/by-nc/4.0/).)
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- 2022
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26. Successful response of vulvar lichen sclerosus with NB-UVB.
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Garrido-Colmenero C, Martínez-Peinado CM, Galán-Gutiérrez M, Barranco-Millán V, and Ruiz-Villaverde R
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- Female, Humans, Carcinoma, Squamous Cell, Ultraviolet Therapy, Vulvar Diseases, Vulvar Lichen Sclerosus diagnosis, Vulvar Neoplasms
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- 2021
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27. Pervasive RNA Regulation of Metabolism Enhances the Root Colonization Ability of Nitrogen-Fixing Symbiotic α-Rhizobia.
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García-Tomsig NI, Robledo M, diCenzo GC, Mengoni A, Millán V, Peregrina A, Uceta A, and Jiménez-Zurdo JI
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- RNA metabolism, Symbiosis, Nitrogen metabolism, Ecosystem, Medicago sativa microbiology, RNA, Messenger metabolism, Rhizobium genetics, Sinorhizobium meliloti genetics
- Abstract
The rhizosphere and rhizoplane are nutrient-rich but selective environments for the root microbiome. Here, we deciphered a posttranscriptional network regulated by the homologous trans -small RNAs (sRNAs) AbcR1 and AbcR2, which rewire the metabolism of the nitrogen-fixing α-rhizobium Sinorhizobium meliloti during preinfection stages of symbiosis with its legume host alfalfa. The LysR-type regulator LsrB, which transduces the cell redox state, is indispensable for AbcR1 expression in actively dividing bacteria, whereas the stress-induced transcription of AbcR2 depends on the alternative σ factor RpoH1. MS2 affinity purification coupled with RNA sequencing unveiled exceptionally large and overlapping AbcR1/2 mRNA interactomes, jointly representing ⁓6% of the S. meliloti protein-coding genes. Most mRNAs encode transport/metabolic proteins whose translation is silenced by base pairing to two distinct anti-Shine Dalgarno motifs that function independently in both sRNAs. A metabolic model-aided analysis of the targetomes predicted changes in AbcR1/2 expression driven by shifts in carbon/nitrogen sources, which were confirmed experimentally. Low AbcR1/2 levels in some defined media anticipated overexpression growth phenotypes linked to the silencing of specific mRNAs. As a proof of principle, we confirmed AbcR1/2-mediated downregulation of the l-amino acid AapQ permease. AbcR1/2 interactomes are well represented in rhizosphere-related S. meliloti transcriptomic signatures. Remarkably, a lack of AbcR1 specifically compromised the ability of S. meliloti to colonize the root rhizoplane. The AbcR1 regulon likely ranks the utilization of available substrates to optimize metabolism, thus conferring on S. meliloti an advantage for efficient rhizosphere/rhizoplane colonization. AbcR1 regulation is predicted to be conserved in related α-rhizobia, which opens unprecedented possibilities for engineering highly competitive biofertilizers. IMPORTANCE Nitrogen-fixing root nodule symbioses between rhizobia and legume plants provide more than half of the combined nitrogen incorporated annually into terrestrial ecosystems, rendering plant growth independent of environmentally unfriendly chemical fertilizers. The success of symbiosis depends primarily on the capacity of rhizobia to establish competitive populations in soil and rhizosphere environments. Here, we provide insights into the regulation and architecture of an extensive RNA posttranscriptional network that fine-tunes the metabolism of the alfalfa symbiont S. meliloti, thereby enhancing the ability of this beneficial bacterium to colonize nutrient-rich but extremely selective niches, such as the rhizosphere of its host plant. This pervasive RNA regulation of metabolism is a major adaptive mechanism, predicted to operate in diverse rhizobial species. Because RNA regulation relies on modifiable base-pairing interactions, our findings open unexplored avenues for engineering the legumes rhizobiome within sustainable agricultural practices.
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- 2021
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28. EPILAT-IRA Study: A contribution to the understanding of the epidemiology of acute kidney injury in Latin America.
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Lombardi R, Ferreiro A, Claure-Del Granado R, Burdmann EA, Rosa-Diez G, Yu L, Younes-Ibrahim M, Carlino C, Chávez-Iñiguez JS, Pereira MB, Varela CF, Zamoner W, Janiques D, Lecueder S, Cerrón-Millán V, and Cueto-Manzano A
- Subjects
- Acute Kidney Injury therapy, Adolescent, Adult, Aged, Caribbean Region epidemiology, Disease-Free Survival, Humans, Latin America epidemiology, Male, Middle Aged, Prospective Studies, Survival Rate, Acute Kidney Injury mortality, Hospital Mortality, Renal Replacement Therapy
- Abstract
Introduction: Epidemiology of acute kidney injury (AKI) is highly dependent on patient characteristics, context and geography. Considering the limited information in Latin America and the Caribbean, we performed a study with the aim to contribute to improve its better understanding., Methods: Observational, prospective, longitudinal, multinational cohort study addressed to determine risk factors, clinical profile, process of care and outcomes of AKI in the region. Patients meeting KDIGO AKI definition were included over a 9-month period and designated community or hospital-acquired. De-identified clinical and lab data were entered in a specifically designed on-line platform. Co-variables potentially linked to AKI onset, in-hospital and 90-days mortality, were recorded and correlated using a multiple logistic regression model., Results: Fifty-seven physicians from 15 countries provided data on 905 patients, most with acceptable basic needs coverage. Median age 64 (50-74) yrs; most of them were male (61%) and mestizos (42%). Comorbidities were present in 77%. AKI was community-acquired in 62%. Dehydration, shock and nephrotoxic drugs were the commonest causes. During their process of care, 77% of patients were assessed by nephrologists. Kidney replacement therapy (KRT) was performed in 29% of cases. In-hospital mortality was 26.5% and independently associated to older age, chronic liver disease, hypotension, shock, cardiac disturbances, hospital-acquired sepsis, KRT and mechanical ventilation. At 90-days follow up partial or complete renal recovery was 81% and mortality 24%., Conclusions: AKI was mainly community-acquired, in patients with comorbidities and linked to fluid loss and nephrotoxic drugs. Mortality was high and long-term follow up poor. Notwithstanding, the study shows partially the situation in the participant countries rather than the actual epidemiology of AKI in Latin America and Caribbean, a pending and needed task., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2019
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29. Changes in the soil bacterial community along a pedogenic gradient.
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Sánchez-Marañón M, Miralles I, Aguirre-Garrido JF, Anguita-Maeso M, Millán V, Ortega R, García-Salcedo JA, Martínez-Abarca F, and Soriano M
- Subjects
- Altitude, Bacteria genetics, Computational Biology, Plants, RNA, Bacterial, RNA, Ribosomal, 16S, Spain, Soil chemistry, Soil classification, Soil Microbiology
- Abstract
Current research on the influence of environmental and physicochemical factors in shaping the soil bacterial structure has seldom been approached from a pedological perspective. We studied the bacterial communities of eight soils selected along a pedogenic gradient at the local scale in a Mediterranean calcareous mountain (Sierra de María, SE Spain). The results showed that the relative abundance of Acidobacteria, Canditate division WPS-1, and Armatimonadetes decreased whereas that of Actinobacteria, Bacteroidetes, and Proteobacteria increased from the less-developed soils (Leptosol) to more-developed soils (Luvisol). This bacterial distribution pattern was also positively correlated with soil-quality parameters such as organic C, water-stable aggregates, porosity, moisture, and acidity. In addition, at a lower taxonomic level, the abundance of Acidobacteria Gp4, Armatimonadetes_gp4, Solirubrobacter, Microvirga, Terrimonas, and Nocardioides paralleled soil development and quality. Therefore, our work indicates that the composition of bacterial populations changes with pedogenesis, which could be considered a factor influencing the communities according to the environmental and physicochemical conditions during the soil formation.
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- 2017
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30. A conserved α-proteobacterial small RNA contributes to osmoadaptation and symbiotic efficiency of rhizobia on legume roots.
- Author
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Robledo M, Peregrina A, Millán V, García-Tomsig NI, Torres-Quesada O, Mateos PF, Becker A, and Jiménez-Zurdo JI
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- Adaptation, Physiological, Conserved Sequence, Medicago sativa physiology, Osmosis, Plant Roots microbiology, Plant Roots physiology, RNA metabolism, RNA, Bacterial genetics, Sinorhizobium meliloti genetics, Medicago sativa microbiology, RNA, Bacterial metabolism, Sinorhizobium meliloti physiology, Symbiosis
- Abstract
Small non-coding RNAs (sRNAs) are expected to have pivotal roles in the adaptive responses underlying symbiosis of nitrogen-fixing rhizobia with legumes. Here, we provide primary insights into the function and activity mechanism of the Sinorhizobium meliloti trans-sRNA NfeR1 (Nodule Formation Efficiency RNA). Northern blot probing and transcription tracking with fluorescent promoter-reporter fusions unveiled high nfeR1 expression in response to salt stress and throughout the symbiotic interaction. The strength and differential regulation of nfeR1 transcription are conferred by a motif, which is conserved in nfeR1 promoter regions in α-proteobacteria. NfeR1 loss-of-function compromised osmoadaptation of free-living bacteria, whilst causing misregulation of salt-responsive genes related to stress adaptation, osmolytes catabolism and membrane trafficking. Nodulation tests revealed that lack of NfeR1 affected competitiveness, infectivity, nodule development and symbiotic efficiency of S. meliloti on alfalfa roots. Comparative computer predictions and a genetic reporter assay evidenced a redundant role of three identical NfeR1 unpaired anti Shine-Dalgarno motifs for targeting and downregulation of translation of multiple mRNAs from transporter genes. Our data provide genetic evidence of the hyperosmotic conditions of the endosymbiotic compartments. NfeR1-mediated gene regulation in response to this cue could contribute to coordinate nutrient uptake with the metabolic reprogramming concomitant to symbiotic transitions., (© 2017 Society for Applied Microbiology and John Wiley & Sons Ltd.)
- Published
- 2017
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31. Rhizobial galactoglucan determines the predatory pattern of Myxococcus xanthus and protects Sinorhizobium meliloti from predation.
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Pérez J, Jiménez-Zurdo JI, Martínez-Abarca F, Millán V, Shimkets LJ, and Muñoz-Dorado J
- Subjects
- Adaptation, Biological, Bacterial Proteins metabolism, Microbial Viability, Movement, Mutation, Myxococcus xanthus genetics, Myxococcus xanthus metabolism, Quorum Sensing, Sinorhizobium meliloti genetics, Trans-Activators genetics, Trans-Activators metabolism, Antibiosis genetics, Bacterial Proteins genetics, Galactans biosynthesis, Gene Expression Regulation, Bacterial, Glucans biosynthesis, Myxococcus xanthus pathogenicity, Polysaccharides, Bacterial biosynthesis, Sinorhizobium meliloti metabolism
- Abstract
Myxococcus xanthus is a social bacterium that preys on prokaryotic and eukaryotic microorganisms. Co-culture of M. xanthus with reference laboratory strains and field isolates of the legume symbiont Sinorhizobium meliloti revealed two different predatory patterns that resemble frontal and wolf-pack attacks. Use of mutants impaired in the two types of M. xanthus surface motility (A or adventurous and S or social motility) and a csgA mutant, which is unable to form macroscopic travelling waves known as ripples, has demonstrated that both motility systems but not rippling are required for efficient predation. To avoid frontal attack and reduce killing rates, rhizobial cells require a functional expR gene. ExpR regulates expression of genes involved in a variety of functions. The use of S. meliloti mutants impaired in several of these functions revealed that the exopolysaccharide galactoglucan (EPS II) is the major determinant of the M. xanthus predatory pattern. The data also suggest that this biopolymer confers an ecological advantage to rhizobial survival in soil, which may have broad environmental implications., (© 2014 Society for Applied Microbiology and John Wiley & Sons Ltd.)
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- 2014
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32. Independent activity of the homologous small regulatory RNAs AbcR1 and AbcR2 in the legume symbiont Sinorhizobium meliloti.
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Torres-Quesada O, Millán V, Nisa-Martínez R, Bardou F, Crespi M, Toro N, and Jiménez-Zurdo JI
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- ATP-Binding Cassette Transporters genetics, ATP-Binding Cassette Transporters metabolism, Down-Regulation, Gene Expression Regulation, Bacterial, Mutation, Nucleic Acid Conformation, RNA Stability, RNA, Bacterial chemistry, RNA, Bacterial genetics, RNA, Small Untranslated chemistry, RNA, Small Untranslated genetics, Sinorhizobium meliloti metabolism, Symbiosis genetics, Fabaceae microbiology, RNA, Bacterial physiology, RNA, Small Untranslated physiology, Sinorhizobium meliloti genetics
- Abstract
The legume symbiont Sinorhizobium meliloti expresses a plethora of small noncoding RNAs (sRNAs) whose function is mostly unknown. Here, we have functionally characterized two tandemly encoded S. meliloti Rm1021 sRNAs that are similar in sequence and structure. Homologous sRNAs (designated AbcR1 and AbcR2) have been shown to regulate several ABC transporters in the related α-proteobacteria Agrobacterium tumefaciens and Brucella abortus. In Rm1021, AbcR1 and AbcR2 exhibit divergent unlinked regulation and are stabilized by the RNA chaperone Hfq. AbcR1 is transcribed in actively dividing bacteria, either in culture, rhizosphere or within the invasion zone of mature alfalfa nodules. Conversely, AbcR2 expression is induced upon entry into stationary phase and under abiotic stress. Only deletion of AbcR1 resulted into a discrete growth delay in rich medium, but both are dispensable for symbiosis. Periplasmic proteome profiling revealed down-regulation of the branched-chain amino acid binding protein LivK by AbcR1, but not by AbcR2. A double-plasmid reporter assay confirmed the predicted specific targeting of the 5'-untranslated region of the livK mRNA by AbcR1 in vivo. Our findings provide evidences of independent regulatory functions of these sRNAs, probably to fine-tune nutrient uptake in free-living and undifferentiated symbiotic rhizobia.
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- 2013
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33. Bacterial community structure in the rhizosphere of three cactus species from semi-arid highlands in central Mexico.
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Aguirre-Garrido JF, Montiel-Lugo D, Hernández-Rodríguez C, Torres-Cortes G, Millán V, Toro N, Martínez-Abarca F, and Ramírez-Saad HC
- Subjects
- Bacteria genetics, Cluster Analysis, DNA, Bacterial chemistry, DNA, Bacterial genetics, DNA, Ribosomal chemistry, DNA, Ribosomal genetics, Mexico, Molecular Sequence Data, Phylogeny, RNA, Ribosomal, 16S genetics, Seasons, Sequence Analysis, DNA, Bacteria classification, Bacteria isolation & purification, Biota, Cactaceae microbiology, Plant Roots microbiology, Rhizosphere
- Abstract
The nature reserve of Tehuacan-Cuicatlan in central Mexico is known for its diversity and endemism mainly in cactus plants. Although the xerophytic flora is reasonably documented, the bacterial communities associated with these species have been largely neglected. We assessed the diversity and composition of bacterial communities in bulk (non-rhizospheric) soil and the rhizosphere of three cactus plant species: Mammillaria carnea, Opuntia pilifera and Stenocereus stellatus, approached using cultivation and molecular techniques, considering the possible effect of dry and rainy seasons. Cultivation-dependent methods were focused on putative N(2)-fixers and heterotrophic aerobic bacteria, in the two media tested the values obtained for dry season samples grouped together regardless of the sample type (rhizospheric or non-rhizospheric), these groups also included the non-rhizospheric sample for rainy season, on each medium. These CFU values were smaller and significantly different from those obtained on rhizospheric samples from rainy season. Genera composition among isolates of the rhizospheric samples was very similar for each season, the most abundant taxa being α-Proteobacteria, Actinobacteria and Firmicutes. Interestingly, the genus Ochrobactrum was highly represented among rhizospheric samples, when cultured in N-free medium. The structure of the bacterial communities was approached with molecular techniques targeting partial 16S rRNA sequences such as denaturing gradient gel electrophoresis and serial analysis of ribosomal sequence tags. Under these approaches, the most represented bacterial phyla were Actinobacteria, Proteobacteria and Acidobacteria. The first two were also highly represented when using isolation techniques.
- Published
- 2012
- Full Text
- View/download PDF
34. Characterization of novel antibiotic resistance genes identified by functional metagenomics on soil samples.
- Author
-
Torres-Cortés G, Millán V, Ramírez-Saad HC, Nisa-Martínez R, Toro N, and Martínez-Abarca F
- Subjects
- Amino Acid Sequence, Bacteria genetics, Bacterial Proteins genetics, Bacterial Proteins metabolism, DNA, Bacterial genetics, Escherichia coli drug effects, Gene Library, Genes, Bacterial, Microbial Sensitivity Tests, Molecular Sequence Data, Phylogeny, Sinorhizobium meliloti drug effects, Soil analysis, Tetrahydrofolate Dehydrogenase genetics, Trimethoprim pharmacology, Bacteria enzymology, Metagenomics, Soil Microbiology, Tetrahydrofolate Dehydrogenase metabolism, Trimethoprim Resistance genetics
- Abstract
The soil microbial community is highly complex and contains a high density of antibiotic-producing bacteria, making it a likely source of diverse antibiotic resistance determinants. We used functional metagenomics to search for antibiotic resistance genes in libraries generated from three different soil samples, containing 3.6 Gb of DNA in total. We identified 11 new antibiotic resistance genes: 3 conferring resistance to ampicillin, 2 to gentamicin, 2 to chloramphenicol and 4 to trimethoprim. One of the clones identified was a new trimethoprim resistance gene encoding a 26.8 kDa protein closely resembling unassigned reductases of the dihydrofolate reductase group. This protein, Tm8-3, conferred trimethoprim resistance in Escherichia coli and Sinorhizobium meliloti (γ- and α-proteobacteria respectively). We demonstrated that this gene encoded an enzyme with dihydrofolate reductase activity, with kinetic constants similar to other type I and II dihydrofolate reductases (K(m) of 8.9 µM for NADPH and 3.7 µM for dihydrofolate and IC(50) of 20 µM for trimethoprim). This is the first description of a new type of reductase conferring resistance to trimethoprim. Our results indicate that soil bacteria display a high level of genetic diversity and are a reservoir of antibiotic resistance genes, supporting the use of this approach for the discovery of novel enzymes with unexpected activities unpredictable from their amino acid sequences., (© 2011 Society for Applied Microbiology and Blackwell Publishing Ltd.)
- Published
- 2011
- Full Text
- View/download PDF
35. [Intestinal Campylobacter jejuni infection in Chilean infants].
- Author
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Prado V, Martínez J, Reyes L, Ducheylard M, Bercovich M, Millán V, Dib G, and Alvarez S
- Subjects
- Chile, Feces microbiology, Humans, Infant, Infant, Newborn, Seasons, Campylobacter Infections microbiology, Campylobacter fetus isolation & purification, Diarrhea, Infantile microbiology
- Published
- 1985
36. [Geographic variations of the isolation of Aeromonas hydrophila in infants with acute infantile diarrhea and a healthy population].
- Author
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Trucco O, Prado V, Siri MT, Dib G, Millán V, Martínez J, Reyes L, and Mamani N
- Subjects
- Aeromonas pathogenicity, Anti-Bacterial Agents pharmacology, Chile, Drug Resistance, Microbial, Enterotoxins analysis, Female, Humans, Infant, Male, Aeromonas isolation & purification, Diarrhea, Infantile microbiology
- Published
- 1987
37. [Clinical applications of the study of neuroendocrinologic rhythms].
- Author
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Cabranes JA, Millán V, Rodríguez C, and Almoguera I
- Subjects
- Adrenocorticotropic Hormone metabolism, Depression metabolism, Follicle Stimulating Hormone metabolism, Growth Hormone metabolism, Humans, Luteinizing Hormone metabolism, Prolactin metabolism, Thyrotropin metabolism, Circadian Rhythm, Endocrine Glands metabolism, Endocrine System Diseases metabolism
- Published
- 1987
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