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Since April 2024, sporadic infections with highly pathogenic avian influenza (HPAI) A(H5) viruses have been detected among dairy farm workers in the United States. To date, infections have mostly been detected through worker monitoring, and have been mild despite the possibility of more severe illness. During June–August 2024, CDC collaborated with the Michigan Department of Health and Human Services and the Colorado Department of Public Health and Environment to implement cross-sectional serologic surveys to ascertain the prevalence of recent infection with HPAI A(H5) virus among dairy workers. In both states, a convenience sample of persons who work in dairies was interviewed, and blood specimens were collected. Among 115 persons, eight (7%; 95% CI = 3.6%–13.1%) had serologic evidence of recent infection with A(H5) virus; all reported milking cows or cleaning the milking parlor. Among persons with serologic evidence of infection, four recalled being ill around the time cows were ill; symptoms began before or within a few days of A(H5) virus detections among cows. This finding supports the need to identify and implement strategies to prevent transmission among dairy cattle to reduce worker exposures and for education and outreach to dairy workers concerning prevention, symptoms, and where to seek medical care if the workers develop symptoms. Timely identification of infected herds can support rapid initiation of monitoring, testing, and treatment for human illness, including mild illness, among exposed dairy workers. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. Endemic infectious diseases remain a major challenge for dairy producers worldwide. For effective disease control programs, up-to-date prevalence estimates are of utmost importance. The objective of this study was to estimate the herd-level prevalence of bovine leukemia virus (BLV), Salmonella enterica ssp. enterica serovar Dublin (Salmonella Dublin), and Neospora caninum in dairy herds in Alberta, Canada, using a serial cross-sectional study design. Bulk tank milk samples from all Alberta dairy farms were collected 4 times, in December 2021 (n = 489), April 2022 (n = 487), July 2022 (n = 487), and October 2022 (n = 480), and tested for antibodies against BLV, Salmonella Dublin, and N. caninum using ELISA. Herd-level apparent prevalence was calculated as positive herds divided by total tested herds at each time point. A mixed-effect modified Poisson regression model was employed to assess the association of prevalence with region, herd size, herd type, and type of milking system. Apparent prevalence of BLV was 89.4%, 88.7%, 86.9%, and 86.9% in December, April, July, and October, respectively, whereas for Salmonella Dublin apparent prevalence was 11.2%, 6.6%, 8.6%, and 8.5%, and for N. caninum apparent prevalence was 18.2%, 7.4%, 7.8%, and 15.0%. For BLV, Salmonella Dublin, and N. caninum , a total of 91.7%, 15.6%, and 28.1% of herds, respectively, were positive at least once, whereas 82.5%, 3.6%, and 3.0% of herds were ELISA positive at all 4 times. Compared with the north region, central Alberta had a high prevalence (prevalence ratio [PR] = 1.13) of BLV antibody-positive herds, whereas south Alberta had a high prevalence (PR = 2.56) of herds positive for Salmonella Dublin antibodies. Furthermore, central (PR = 0.52) and south regions (PR = 0.46) had low prevalence of N. caninum -positive herds compared with the north. Hutterite colony herds were more frequently BLV positive (PR = 1.13) but less frequently N. caninum -positive (PR = 0.47). Large herds (>7,200 L/d milk delivered ∼>250 cows) were 1.1 times more often BLV positive, whereas small herds (≤3,600 L/d milk delivered ∼≤125 cows) were 3.2 times more often N. caninum positive. For Salmonella Dublin, Hutterite colony herds were less frequently (PR = 0.07) positive than non-colony herds only in medium and large strata but not in small stratum. Moreover, larger herds were more frequently (PR = 2.20) Salmonella Dublin-positive than smaller herds only in non-colony stratum but not in colony stratum. Moreover, N. caninum prevalence was 1.6 times higher on farms with conventional milking systems compared with farms with an automated milking system. These results provide up-to-date information of the prevalence of these infections that will inform investigations of within-herd prevalence of these infections and help in devising evidence-based disease control strategies. [ABSTRACT FROM AUTHOR]

Donkey breeding in both Europe and worldwide is gaining a momentum. An increasingly common reason for breeding donkeys is milk production. Unfortunately, donkey belongs to a group of rare domestic animals and systematic selection for their milk production has almost never been made. Donkey is most commonly used as a working animal. In order to examine the basic morphometric parameters of the udders of dairy donkeys, research was carried out on 15 dairy donkeys, bred on the farm of the Special Nature Reserve Zasavica in Zasavica near Sremska Mitrovica, in the Republic of Serbia. Donkeys are housed in groups in pens, without tethering. After milking, the foals were with them, and during milking mothers had visual contact with their foals. Milking was performed manually, and the amount of milk was determined by pouring it into a plastic measuring cup. Two milkings were performed two h apart. The average amount of milk obtained per day per donkey is 346.6 ml. There is a large variation interval between the minimum and maximum amount of milk obtained which indicates the heterogeneity of the individuals used in production and that there was no selection work for this type of production. The average amount of milk is smaller compared to Zapata where selection for milk production was made. Morphological characteristics of the udder differ statistically very significantly before and after milking, especially in relation to udder circumference, teat circumference and teat width. No statistically significant difference was found for the amount of milk obtained from left or right mammary complex. [ABSTRACT FROM AUTHOR]

Good hygienic milking practices including the disinfection of the cow teat skin before and after milking aimed at preventing the occurrence of new intramammary infections (IMI) in dairy cows. This practice is generally performed using disinfectants, and in the current context of attention to the emergence of resistances, it is of greatest interest to evaluate alternative solutions that can expand treatment options. We assessed the efficacy of a pre-dipping and a post-dipping product based on the bacteriocin-containing culture of Lactococcus cremoris strain FT27, as compared to commercial disinfectants. FT27 was selected for the bactericidal activity in vitro against food pathogens. In the present study, it also revealed a high bactericidal activity against the main mastitis pathogens, most likely related to Nisin A production, according to genomic and proteomic analysis. The lactococcus-based preparations were applied in two commercial dairy farms in Northern Italy. Overall, 298 dairy cows were enrolled, 141 in the treated group (TR), and 157 in the control group (CTR). The cows were sampled at quarter level every two weeks for 3 months. During the trial, all cows showed a good health status. The hygiene level for udder, flanks and legs was generally good (on average < 3 score); the udder hygiene was significantly worse (P < 0.01) in the CTR group. The teat apex hyperkeratosis was overall low (on average < 2 score). We recorded no significant differences between the two experimental groups in the Somatic Cell Count (SCC) values and the bacteriological results. The overall frequency of new IMI was low, ranging 0.6 − 0.5% for S. aureus in the TR or CTR group respectively, to 2.6–4.4% for NASM. Regarding Str. spp., the new IMI accounted for 1.7% or 1.9% in the TR or CTR group, respectively. Notably, the incidence of S. aureus new IMI did not increase during the study, even though neither of the two herds segregated the positive cows. The non-inferiority test showed that the Lactococcus-based pre- and post-dipping products had an efficacy comparable to proven commercial disinfectants in maintaining udder health and preventing new IMI, thus representing a possible alternative to current teat dip products. [ABSTRACT FROM AUTHOR]

Background: Despite the widespread adoption of TMR or PMR and the formulas designed to sufficiently cover the cows' requirements, individual dairy cows' milk production varies significantly. The liver is one of the most important organs in cow lactation metabolism and plays an essential role in the initiation of lactation. Objectives: This study aimed to investigate the potential key genes in the liver contributing to the different milk production. Methods: We enrolled 64 cows and assigned them to high or low milk yield (MY) groups according to their first 3 weeks of milk production. We performed RNAseq for 35 liver samples with 18 from prepartum and 17 from postpartum cows. Results: The continuous milk yield observation showed a persistently higher milk yield in high MY cows than low MY cows in the first 3 weeks. High MY cows showed better feed conversion efficiency. We identified 795 differentially expressed genes (DGEs) in the liver of high MY cows compared with low MY cows, with up-regulated genes linked to morphogenesis and development pathways. Weighted gene co-expression network analysis (WGCNA) revealed four gene modules positively correlating with milk yield, and protein and lactose yield (p < 0.05). Using the intersected genes between the four gene modules and DEGs, we constructed the linear mixed-effects models and identified six hub genes positively associated and two hub genes negatively associated with milk yield (Coefficients > 0.25, p < 0.05). Random forest machine learning model training based on these eight hub genes could efficiently predict the milk yield (p < 0.001, R2 = 0.946). Interestingly, the expression patterns of these eight hub genes remained remarkably similar before and after parturition. Conclusions: The present study indicated the critical role of liver in milk production. Activated processes involved in morphogenesis and development in liver may contribute to the higher milk production. Eight hub genes identified in this study may provide genetic research materials for dairy cow breeding. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. Moving from conventional (CMS) to automatic (AMS) milking systems could affect milk quality. Moreover, the type and preservation methods of the forages used in the TMR, such as alfalfa hay (HTMR) or corn silage (STMR) have been demonstrated to modify milk composition. Thus, this study investigated the effect of implementing AMS and different diet forage types on the quality of Italian Holstein-Friesian bulk milk. Milk samples (n = 168) were collected monthly from 21 commercial farms in northern Italy during a period of 8 mo. Farms were categorized into 4 groups according to their milking system (CMS vs. AMS) and diet forage type (HTMR vs. STMR). Milk quality data were analyzed through the mixed procedure for repeated measurement of SAS with the milking system, diet forage type, and sampling day as fixed effects. Milking through the AMS led to lower milk fat, freezing point, and β-LG A; longer coagulation time; and higher K content, pH, and β-LG B than CMS. Cows fed STMR produced milk with greater fat, protein, casein, Mg content, titratable acidity, and β-LG A, but with reduced curd firming time, freezing point, and β-LG B than those fed HTMR. In conclusion, milk quality is not only altered by the diet's forage type and characteristics but also by the milking system. [ABSTRACT FROM AUTHOR]

Milk can be contaminated with organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and polycyclic aromatic hydrocarbons (PAHs), which are known as endocrine disruptors (EDCs). However, the relationship between the lactation period of cows and the residues of EDCs is unknown. The aim of this study was to determine the relationship between the lactation period in cows and EDCs residues such as OCPs, PCBs, PBDEs, and PAHs. Milk collected from cows during each lactation period was analyzed in terms of fat content and EDC residues. One or more contaminants were detected in almost all (98%) of the milk taken in three lactation periods. Three PCBs and 11 PAHs were found together in 1st lactation period, and one more PAH was added to them in other periods. However, the sample rate exceeded the maximum residue limit of 48%, 10%, and 16% in the first, second and third lactation periods, respectively. Also, it was seen that the riskiest period was the first lactation period, followed by the third and second periods. The same ranking is valid for the fat content in milk. Thus, it was concluded that the excretion of lipophilic EDCs in milk is related to the fat content in milk, and the higher the fat content in milk, the more lipophilic EDCs are excreted in milk.

BACKGROUND: Including seaweed in cattle feed has gained increased interest, but it is important to take into account that the concentration of toxic metals, especially arsenic, is high in seaweed. This study investigated the arsenic species in milk from seaweed‐fed cows. RESULTS: Total arsenic in milk of control diets (9.3 ± 1.0 μg As kg−1, n = 4, dry mass) was significantly higher than seaweed‐based diet (high‐seaweed diet: 7.8 ± 0.4 μg As kg−1, P < 0.05, n = 4, dry mass; low‐seaweed diet: 6.2 ± 1.0 μg As kg−1, P < 0.01, n = 4, dry mass). Arsenic speciation showed that the main species present were arsenobetaine (AB) and arsenate (As(V)) (37% and 24% of the total arsenic, respectively). Trace amounts of dimethylarsinic acid (DMA) and arsenocholine (AC) have also been detected in milk. Apart from arsenate being significantly lower (P < 0.001) in milk from seaweed‐fed cows than in milk from the control group, other arsenic species showed no significant differences between groups. CONCLUSION: The lower total arsenic and arsenate in seaweed diet groups indicates a possible competition of uptake between arsenate and phosphate, and the presence of AC indicates that a reduction of AB occurred in the digestive tract. Feeding a seaweed blend (91% Ascophyllum nodosum and 9% Laminaria digitata) does not raise As‐related safety concerns for milk. © 2024 The Authors. Journal of The Science of Food and Agriculture published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

On the basis of current growth trajectories, pasture-based dairies of the future are likely to be bigger, have higher stocking rates and feed more concentrate to cows. This review uses the five-domains framework to consider risks to the welfare of dairy cows in these larger intensified pasture-based production systems. The factors considered in this review can be broadly categorised as (1) emerging welfare risks that can be managed, (2) emerging welfare risks that require research to be managed, or (3) persisting and/or exacerbated welfare risks. First, large herds could be subject to welfare risks associated with more stock per labour unit, longer milking times and longer distances walked to and from the dairy. To counter this, the time that cows in large herds spend off pasture can be reduced by splitting the herd into several more manageable groups, and animal-monitoring technologies can help identify health challenges with a reduced stockperson to animal ratio. Cow body condition and productivity can be maintained at high stocking rates by improving pasture production and feeding a higher proportion of concentrate. The risk of ruminal acidosis may then be reduced by appropriate transition feeding regimes and rumen buffers. Second, ensuring social stability and reducing competition may become difficult as herd sizes increase and feeding becomes more intensive. The resulting variability in feed intake, increased agonistic behaviour and social stress present emerging risks to cow welfare. Research is needed to better understand the social behaviour of cows in large intensive pasture-based herds, and how the design of the pre-milking area, the feeding pad and pasture feeding regimes (i.e. quantity and timing of pasture allocation) can improve accessibility for more vulnerable animals. Finally, intensive pasture-based dairies of the future will continue to face welfare challenges relating to lameness, mastitis and cull-cow management, whereas risks due to environmental exposure may be exacerbated by the removal of shelterbelts to facilitate irrigation. These require continued efforts in research (e.g. ways of incorporating shelter into intensive grazing systems), development (e.g. pathway to market for aged beef) and extension (e.g. improved record keeping and benchmarking of lameness and mastitis). Pasture-based dairies of the future are likely to be bigger, have higher stocking rates and feed more concentrate. This review uses the five-domains framework to consider risks to the welfare of dairy cows in these larger pasture-based systems. Risks to cow welfare are broadly categorised as (1) emerging welfare risks that can be managed, (2) emerging welfare risks that require research to be managed, or (3) persisting and/or exacerbated welfare risks. Suggestions are made for future research, development and extension. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. Bovine leukosis is prevalent in the North American dairy industry, and its effect on animal health and production is widely documented. However, not all bovine leukemia virus (BLV)–infected animals transmit the virus equally. Animals with high proviral loads (HPL) of BLV are associated with higher transmission risks, and therefore, their removal may reduce transmission and eventually within-herd prevalence. We aimed to evaluate the impact of selectively removing HPL cows on the within-herd BLV prevalence and incidence rate of BLV infection in 10 dairy herds. Annual blood or milk samples (or both) were collected from adult cows over 3 yr. Positivity with BLV were determined by ELISA tests, and proviral loads in blood of BLV-positive animals were estimated with BLV SS1 quantitative PCR assays. Herd managers were encouraged to consider the proviral load when making culling decisions and implement BLV control practices. Cows with high proviral load had the highest relative risk of removal, indicating the farmers prioritized HPL cows for culling. The within-herd BLV prevalence decreased significantly in 4 herds, whereas BLV incidence rate decreased in 9 herds. Over the 3 yr, the proviral load demonstrated a relatively stable level, suggesting a single proviral load test in an adult cow may suffice to make culling decisions. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. The aim of this systematic review was to summarize the literature assessing the effects of weaning practices on performance, behavior, and health of dairy calves. Only published, peer-reviewed articles written in English and specifically assessing the effects of weaning treatments on dairy calves were eligible for inclusion. Studies had to include 2 or more treatment groups that addressed at least one of 4 interventions: weaning age (i.e., when milk was fully removed), weaning duration (i.e., number of days from start of milk reduction to when milk was fully removed), weaning criteria (e.g., age vs. intake), or alternative weaning methods (e.g., water dilution). Outcome measures could include starter intake, growth (BW or ADG), behaviors (5 specific sucking behaviors; play behavior; lying behavior; vocalizations; unrewarded visits to an automated milk feeder), and health (mortality rate, diarrhea, and respiratory illness). We conducted 3 targeted searches using Web of Science and PubMed. The articles underwent a 2-step screening process, resulting in a final sample of 44 studies. The majority of studies investigated weaning age (n = 22), followed by weaning duration (n = 13), weaning criteria (n = 9), and other weaning methods such as dilution, linear versus step-down milk reduction, or meal-based approaches (n = 6). There was consensus for positive effects (or at least no negative effects) on overall growth of calves weaned at later ages, over longer durations, based on starter intake, or weaned using step-down or meal-based milk removal approaches. This is despite reduced starter intake in calves weaned at later ages; most studies found improved starter intake in calves weaned over longer durations. Weaning based on starter intake had superior growth and feed intakes compared with calves weaned at a fixed earlier age. Few studies assessed interactive effects of weaning method and milk allowance. However, weaning after 8 wk appears to support superior weight gain, provided preweaning milk allowances are adequate (above 6 L/d). Weaning can lead to hunger-related behaviors and reduced welfare, yet only half of the studies assessed the effects of weaning method on calf behavior. Weaning at later ages can reduce signs of hunger (based on unrewarded visits to the milk feeder), but it is unclear if weaning over longer durations or weaning by starter intake reduces or prolongs hunger. There was little consensus among the few studies that measured oral behaviors of calves. Positive welfare indicators, such as play behavior, were rarely measured, yet are crucial to understanding the emotional states of calves during this potentially stressful diet transition. Health was rarely the primary objective of the study, with low sample sizes to conduct statistical comparisons. Overall, there remains significant knowledge gaps in our understanding of how best to wean calves. A successful weaning program must minimize signs of hunger while promoting high growth and feed intakes, so we encourage future work to include behavioral indicators of hunger and positive welfare to evaluate how weaning methods are experienced by the calf. [ABSTRACT FROM AUTHOR]

The list of standard abbreviations for JDS is available at adsa.org/jds-abbreviations-24. Nonstandard abbreviations are available in the Notes. Bovine digital dermatitis (BDD) is an important cause of lameness in dairy cows worldwide. However, very little is known about this disease in Australian herds, which are predominantly managed on pasture. The primary objectives of this cross-sectional study were to describe the presence and prevalence of BDD in Australian dairy herds and to characterize the microbiota of healthy skin and M4 lesions of BDD-affected, pasture-managed cows. Cows from 71 dairy herds were examined at milking time to identify the presence of BDD lesions. True prevalence was estimated using Bayesian methods with informative priors for sensitivity and specificity. Biopsy samples (n = 60) were collected from cows with and without BDD lesions in 7 pasture-based herds. The microbiota in the superficial and deep strata of each tissue biopsy were characterized via sequencing of the V3–V4 region of the bacterial ribosomal RNA gene. Lesions were detected in 1,817 (11.5%) of 15,813 cows and in 68 of 71 (95.8%) herds. The median herd-level apparent and true prevalences of BDD were 8.5% and 18.1%, respectively, but prevalences varied considerably between farms. On farms with BDD, M4 lesions accounted for 70% to 100% of all lesions (interquartile range = 95.1%–100%, median = 100%); M2 lesions (i.e., large ulcerative lesions) were observed at low prevalence (<2.2%) in the few herds (7/71, 9.9%) where they were found. There was a significant difference in the composition of the microbiota between healthy skin and M4 lesions but not between superficial and deep tissue layers. Several gut- and effluent-associated bacterial taxa, including Lentimicrobium and Porphyromonas , which have previously been associated with BDD, were abundant in BDD lesions but not in control biopsies. Our study supports the idea that such taxa are involved in, although possibly not essential to, lesion development and persistence in pasture-managed cows in Australia. Our results also suggest that Dichelobacter may contribute to the disease process. We conclude that BDD is likely to occur in most Australian dairy farms, but that further studies are needed to identify its effect on cow welfare and productivity. Further investigation of the etiology of BDD in Australian dairy herds is also necessary to inform prevention and control strategies. [ABSTRACT FROM AUTHOR]

The article proposes an assessment of the quantitative parameters of the crop using the analysis of mechanical vibrations. A demonstration of the investigation is shown for a situation where a support system in the field helps cucumbers grow vertically. The study was carried out by modeling the yield of cucumbers. The greatest attention was paid to the frequencies of natural oscillations. A developed numerical model of the support design is proposed and its natural oscillation frequencies are calculated using the finite element method (FEM). The results were confirmed by a physical experiment that actually simulates a numerical model. [ABSTRACT FROM AUTHOR]

Simple Summary: The primary aim of the study was to evaluate the variability in the number of somatic cells in cisternal and alveolar milk of Polish Holstein-Friesian cows. The obtained results showed that the SCC and the analyzed characteristics exhibited high variability caused by the influence of various environmental and genetic factors. In reference to cisternal and alveolar milk, inconclusive results were obtained, suggesting that the SCC levels in milk collected from different milking phases were similar. The aim of the study was to evaluate the influence of the milking phase on somatic cell count (SCC) in milk obtained from the cisternal and alveolar parts of udders of selected Polish Holstein-Friesian cows. The study also assessed the impact of other genetic and environmental factors on SCC variability in cisternal and alveolar milk, including: the individual cow, lactation stage, age of cow, production level, milking speed, fat-to-protein ratio, and milking type. The research included 15 cows of Polish Holstein-Friesian breed at different ages, lactation stages, and with varying daily milk yield. A total of 210 milk observations were conducted, including 105 for 1 min milking and 105 for 8 min milking. The results obtained in the study indicated that milk obtained during two different milking phases exhibited similar SCC levels (F for LOGSCC = 0.79). The average actual SCC in milk produced by 15 cows in 105 observations for 1 min milking was 219,000 cells/mL, while for 8 min milking it was 229,000 cells/mL. The results were inconclusive, suggesting that SCC in cisternal and alveolar milk must be influenced by factors other than the milking phase. The analysis of variance conducted for this purpose provided the basis for stating a highly statistically significant effect of the individual cow (F for LOGSCC = 147.9), lactation stage (F for LOGSCC = 54.64), age of cow (F for LOGSCC = 12.39), daily production level (F for LOGSCC = 34.49), milking speed (F for LOGSCC = 17.56), and fat-to-protein ratio (F for LOGSCC = 22.99) on the variability of characteristics defining SCC in milk. In summary, SCC is characterized by high variability, influenced by a range of environmental and genetic factors such as the individual cow, lactation stage, age of cow, milking speed, and dietary fat-to-protein ratio. The influence of milking phase (1 min or 8 min) and milking type (morning or evening) should be considered inconclusive based on the entire population studied. For half of the cows, SCC in cisternal milk was higher than in alveolar milk, while for the other half, the situation was reversed. Further observations are required to confirm the hypothesis regarding the extent to which cows' immunological response to bacterial infections is concentrated in the cisternal or alveolar part of the udder under national environmental conditions. [ABSTRACT FROM AUTHOR]

An increasing trend in ancestral and classical inbreeding coefficients as well as inbreeding depression for longevity were found in the German Brown population. In addition, the proportion of US Brown Swiss genes is steadily increasing in German Browns. Therefore, the aim of the present study was to analyze the presence and genomic localization of runs of homozygosity (ROH) in order to evaluate their associations with the proportion of US Brown Swiss genes and survival rates of cows to higher lactations. Genotype data were sampled in 2364 German Browns from 258 herds. The final data set included 49,693 autosomal SNPs. We identified on average 35.996 ± 7.498 ROH per individual with a mean length of 8.323 ± 1.181 Mb. The genomic inbreeding coefficient FROH was 0.122 ± 0.032 and it decreased to 0.074, 0.031 and 0.006, when genomic homozygous segments > 8 Mb (FROH>8), >16 Mb (FROH>16) and >32 Mb (FROH>32) were considered. New inbreeding showed the highest correlation with FROH>32, whereas ancestral inbreeding coefficients had the lowest correlations with FROH>32. The correlation between the classical inbreeding coefficient and FROH was 0.572. We found significantly lower FROH, FROH>4, FROH>8 and FIS for US Brown Swiss proportions <60% compared to >80%. Cows surviving to the 2nd, 4th, 6th, 8th, and 10th lactation had lower genomic inbreeding for FROH and up to FROH>32, which was due to a lower number of ROH and a shorter average length of ROH. The strongest ROH island and consensus ROH shared by 50% of the animals was found on BTA 6 at 85–88 Mb. The genes located in this genomic region were associated with longevity (NPFFR2 and ADAMTS3), udder health and morphology (SLC4A4, NPFFR2, GC and RASSF6), milk production, milk protein percentage, coagulation properties of milk and milking speed (CSN3). On BTA 2, a ROH island was detected only in animals with <60% US Brown Swiss genes. Genes within this region are predominantly important for dual-purpose cattle breeds including Original Browns. For cows reaching more than 9 lactations, an exclusive ROH island was identified on BTA 7 with genes assumed to be associated with longevity. The analysis indicated that genomic homozygous regions important for Original Browns are still present and also ROH containing genes affecting longevity may have been identified. The breeding of German Browns should prevent any further increase in genomic inbreeding and run a breeding program with balanced weights on production, robustness and longevity. [ABSTRACT FROM AUTHOR]

Dairy production on farms is based on properly selected technologies implemented in various areas of the barn and outside the livestock buildings. These technologies are subject to assessment, for example, to determine the possibilities of their further improvement in the given production conditions of the farm. When assessing dairy production technology on a farm, human interests are taken into account, including workload, time and access to modern tools supporting the control of production processes. The aim of this review is to identify and discuss factors in dairy production technologies that may affect the welfare of dairy cattle. The considerations indicate that in the technologies of cow feeding, watering and housing, the priority is to improve the technology in terms of ensuring the comfort of animals using feed, water and a place to rest. However, in the case of the assessment of milking automation, the key importance of increasing human comfort was indicated, taking into account the comfort of cows, which is an additional factor justifying the implementation of technical progress in milking. The assessment of various dairy production technologies on farms is an excellent opportunity to develop discussions on the place of dairy cattle welfare in the sustainable development of farms and the priorities set for improving dairy production. [ABSTRACT FROM AUTHOR]

Milk can be contaminated with organochlorine pesticides (OCPs), polychlorinated biphenyls (PCBs), polybrominated diphenyl ethers (PBDEs), and polycyclic aromatic hydrocarbons (PAHs), which are known as endocrine disruptors (EDCs). However, the relationship between the lactation period of cows and the residues of EDCs is unknown. The aim of this study was to determine the relationship between the lactation period in cows and EDCs residues such as OCPs, PCBs, PBDEs, and PAHs. Milk collected from cows during each lactation period was analyzed in terms of fat content and EDC residues. One or more contaminants were detected in almost all (98%) of the milk taken in three lactation periods. Three PCBs and 11 PAHs were found together in 1st lactation period, and one more PAH was added to them in other periods. However, the sample rate exceeded the maximum residue limit of 48%, 10%, and 16% in the first, second and third lactation periods, respectively. Also, it was seen that the riskiest period was the first lactation period, followed by the third and second periods. The same ranking is valid for the fat content in milk. Thus, it was concluded that the excretion of lipophilic EDCs in milk is related to the fat content in milk, and the higher the fat content in milk, the more lipophilic EDCs are excreted in milk. [ABSTRACT FROM AUTHOR]

The objective of this study was to assess the potential effect of difructose anhydride III (DFAIII) on calcemia, magnesemia, and milking performance in dairy cows. A total of 66 multiparous Holstein cows in late pregnancy (gestation days, mean ± SD = 253.8 ± 2.13 d) were blocked according to their expected calving date and randomly assigned to either receiving no supplementation (control) or receiving 40 g/d of DFAIII (DFA) between −14 and +7 d relative to calving following a complete randomized block design. Cows in the control group received 640 g/d of a pellet containing no DFAIII, whereas DFA cows received the same pellet but containing 6.25% DFAIII. Pellets of each treatment were top-dressed on a daily basis while cows were dry and were fed via an automatic feeding system twice daily (320 g each feeding) during milking. Dry cows were fed once a day, whereas lactating cows were fed twice daily. Dry matter intake was individually monitored on a daily basis. Cows were milked twice daily and milk production and milk protein and fat contents recorded at every milking. Cows were kept on the study until they reached 21 d postpartum. Cows were weighed at dry-off (∼45 d before study enrollment) and twice daily after calving at the exit of the milking parlor. Cows were blood sampled for subsequent determination of serum Ca and Mg concentrations 3 d before the expected calving date and at 6, 12, 24, and 48 h and 7 and 14 d after calving. We found no differences in DMI before calving, but DFA cows consumed more feed than control cows at ∼15 DIM. All cows lost BW after calving, but DFA cows lost slightly less BW during the first 5 DIM than control cows. Cows on DFAIII produced more milk at ∼10 DIM compared with control cows, and DFAIII cows produced more milk protein than control cows 3 d after calving. Serum Ca concentrations were not affected by DFAIII supplementation; however, serum Mg concentrations at 6, 12, and 24 h after calving were greater in DFA than in control cows. In all, DFAIII did not affect postpartum calcemia but improved magnesemia between 6 and 24 h after calving. Milk production in DFA cows was improved around 10 d after calving and milk protein yield after 3 d postpartum compared with control cows. The mechanism leading to increased Mg availability is not clear and warrants further research. [ABSTRACT FROM AUTHOR]

The objective of this study was to describe the nutritional strategies used on Canadian dairy farms with automated milking systems (AMS), both at the feed bunk and the concentrate offered at the AMS, as well as to determine what dietary components and nutrients, as formulated, were associated with milk production and milking behaviors on those farms. Formulated diets (including ingredients and nutrient content) and AMS data were collected from April 1, 2019, until September 30, 2020, on 160 AMS farms (eastern Canada [East] = 8, Ontario [ON] = 76, Quebec [QC] = 22, and western Canada [West] = 54). Both partial mixed ration (PMR) and AMS concentrate samples were collected from May 1 to September 30, 2019, on 169 farms (East = 12, ON = 63, QC = 42, West = 52). We collected AMS milking data for 154 herds. For each farm (n = 161), milk recording data were collected and summarized by farm to calculate average milk yield and components. Multivariable regression models were used to associate herd-level formulated nutrient composition and feeding management practices with milk production and milking behavior. Milk yield (mean ± SD = 37.0 ± 0.3 kg/d) was positively associated with the PMR ether extract (EE) concentration (+0.97 kg/d per percentage point [ p.p. ] increase) and with farms that fed barley silage as their major forage source (n = 16; +2.18 kg/d) as compared with haylage (n = 42), whereas farms that fed corn silage (n = 96; +1.23 kg/d) tended to produce more milk than farms that fed haylage. Greater milk fat content (4.09 ± 0.28%) was associated with a greater PMR-to-AMS concentrate ratio (+0.02 p.p. per unit increase) and total diet net energy for lactation (+0.046 p.p. per 0.1 Mcal/kg increase), but a lesser percentage of NFC of the PMR (−0.016 p.p. per p.p. increase of NFC percentage). Milk protein content (3.38 ± 0.14%) was positively associated with the forage percentage of the PMR (+0.003 p.p. per p.p. increase of forage percentage) and the total diet starch percentage (+0.009 p.p. per p.p. increase of starch percentage), but was negatively associated with farms feeding corn silage (−0.1 p.p. compared with haylage) as their major forage. Greater milking frequency (2.77 ± 0.40 milkings/d) was observed on farms with free-flow cow traffic systems (+0.62 milkings/d) and was positively associated with feed push-up frequency (+0.013 milkings/d per additional feed push-up), but negatively associated with PMR NFC content and forage percentage of the total ration (−0.017 milkings/d per p.p. increase of forage percentage). Lastly, greater milking refusal frequency (1.49 ± 0.82 refusals/d) was observed on farms with free-flow cow traffic systems (+0.84 refusals/d) and farms feeding barley silage (+0.58 refusals/d) than with guided flow and farms feeding either corn silage or haylage, respectively. These data give insight into the ingredients, nutrient formulations and type of diets fed on AMS dairy farms across Canada and the association of those factors with milk production and milking behaviors. [ABSTRACT FROM AUTHOR]

Around 10.5 million tonnes of sheep milk are produced worldwide, and around 3.1 million tonnes in Europe. In Europe, sheep milk production has stagnated slightly in recent years and currently accounts for around 1/3 of the global production. Due to its high content of dry matter, milk fat, total protein and casein, sheep milk is an excellent raw material for cheese production. Milk processing and cheese making are not only economically important, but also part of the cultural heritage based on a long tradition, often taking place in rural and less developed areas. Milk production and quality are very complex and are influenced by numerous factors, the most important of which are breed, feeding and body condition, parity and stage of lactation, milking and udder health, and environmental factors. In addition, different types of stress such as heat stress, nutritional stress, weaning stress, shearing stress, stress of regrouping, etc. have a significant impact on the welfare and health of the sheep as well as on the quantity of milk produced, its chemical composition, processing characteristics and the economic efficiency of the flock. In recent years, due to climate change, there has been an increasing number of studies on the effects of heat stress on the reproductive and production characteristics of sheep, as well as on their welfare and health. In addition, heat stress is often associated to the feed and the lack of water. The effects of stress are particularly pronounced in extensive sheep farming and milk production, which are prevalent in sheep farming. The aim of this paper is to describe the effects of the main types of stress on the quantity and chemical composition of sheep milk and on animal welfare. [ABSTRACT FROM AUTHOR]

Consumers are increasingly interested in foods that offer a well-balanced dietary profile as well as metabolic, physiological, and functional benefits. Dairy fermented products are widely consumed since they provide health benefits via probiotics. However, lactose intolerance, cholesterol content, allergic milk proteins are limiting factors for the consumption of dairy-derived probiotic foods. This problem has led to the exploration of non-dairy fermented probiotic foods derived from cereals, fruits, vegetables and plants. They represent a promising carrier for probiotic microorganisms. In this paper, chemical, microbiological and biological features of traditional non-dairy fermented foods were reviewed, especially by focusing on their probiotic potential. [ABSTRACT FROM AUTHOR]

Animal reproduction biotechniques are important tools for the technological advancement of livestock, as they allow the selection of the reproductive potential of superior quality females and males; however, infectious diseases that have a predilection for the reproductive system can be a hindrance for the use of these technologies. Therefore, the present study aimed to detect Brucella spp. in the ovarian follicular fluid of brucellosis‐positive bovine cows. A total of 47 bovine ovarian follicular fluid aspirates from cows, positive in tests for brucellosis and from Brucella‐positive herd, were submitted to PCR. The primers used in the PCR were specific to the genus Brucella (bcsp31 gene). All 47 bovine aspirates were negative for Brucella spp. 0.00% (95% CI: 0.00–4.00%). Our results demonstrated that Brucella spp. was absent in the ovarian follicular fluid from seropositive cows, which indicates that Brucella spp.‐infected cows could be used for reproductive biotechnologies carried out with follicular aspirates. Future studies are needed to more precisely evaluate the feasibility and safety of using these oocytes from brucellosis‐seropositive cows to transfer embryos to heifers/cows not infected by Brucella, aiming to produce calves free of the infection. [ABSTRACT FROM AUTHOR]

In March 2024, clade 2.3.4.4b H5N1 highly pathogenic avian influenza virus (HPAIV) was detected in dairy cattle in the US, and it was discovered that the virus could be detected in raw milk. Although affected cow’s milk is diverted from human consumption and current pasteurization requirements are expected to reduce or eliminate infectious HPAIV from the milk supply, a study was conducted to characterize whether the virus could be detected by quantitative real-time RT-PCR (qrRT-PCR) in pasteurized retail dairy products and, if detected, to determine whether the virus was viable. From 18 April to 22 April 2024, a total of 297 samples of Grade A pasteurized retail milk products (23 product types) were collected from 17 US states that represented products from 132 processors in 38 states. Viral RNA was detected in 60 samples (20.2%), with qrRT-PCRbased quantity estimates (non-infectious) of up to 5.4log1050% egg infectious doses per mL, with a mean and median of 3.0log10/mL and 2.9log10/mL, respectively. Samples that were positive for type A influenza by qrRT-PCR were confirmed to be clade 2.3.4.4 H5 HPAIV by qrRT-PCR. No infectious virus was detected in any of the qrRT-PCR-positive samples in embryonating chicken eggs. Further studies are needed to monitor the milk supply, but these results provide evidence that the infectious virus did not enter the US pasteurized milk supply before control measures for HPAIV were implemented in dairy cattle. IMPORTANCE Highly pathogenic avian influenza virus (HPAIV) infections in US dairy cattle were first confirmed in March 2024. Because the virus could be detected in raw milk, a study was conducted to determine whether it had entered the retail food supply. Pasteurized dairy products were collected from 17 states in April 2024. Viral RNA was detected in one in five samples, but infectious virus was not detected. This provides a snapshot of HPAIV in milk products early in the event and reinforces that with current safety measures, infectious viruses in milk are unlikely to enter the food supply. [ABSTRACT FROM AUTHOR]

Biochar as a highly promising CO2 adsorbent is of great significance in addressing global warming and promoting human health. Research has shown that nitrogen doping improves the CO2 adsorption performance of biochar, but selecting chemical nitrogen sources such as urea and melamine to prepare nitrogen-doped biochar is not conducive to green production and environmental protection. Therefore, it is necessary to identify a new nitrogen source to enhance the emission reduction characteristics of this process. This study selected corn straw as the raw material and cow manure as a representative protein-based nitrogen source to explore its potential as a urea substitute and reveal the hydrothermal carbonization doping mechanism of different nitrogen sources. The results indicated that in raw materials with the same C/N ratio, biochar prepared from cow manure as the nitrogen source had a better doping effect and CO2 adsorption performance. Moreover, a moderate amount of cow manure was beneficial for efficient nitrogen doping and the adsorption of CO2 by biochar, with a maximum CO2 adsorption performance improvement of 32.7%. Due to the different carbon-nitrogen bonds of the different nitrogen sources, urea was more likely to retain amino groups, while macromolecular protein nitrogen sources tended to retain structural nitrogen. The results of this study provide new ideas and theoretical support for preparing other nitrogen-doped carbon materials derived from biomass. Highlights: • Doping effect of cow manure is stronger than urea under the same C/N value of raw materials. • Moderate addition of cow manure is beneficial for nitrogen doping of biochar. • Small-molecule nitrogen sources help retain amino groups and protein nitrogen sources help retain structural nitrogen. [ABSTRACT FROM AUTHOR]

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Bovine clinical Mastitis (CM) is the most important disease in the dairy industry affecting both animal welfare and farm profitability. Therefore, early and accurate detection of the disease is a valuable timely intervention. In this article, six different machine learning classification algorithms were compared to obtain a prediction model for early detection of the disease. These algorithms are Support Vector Machine, Logistic Regression, Gaussian Naïve Bayes, K-Nearest Neighbor, Classification and Regression Decision Tree, and Random Forest. The algorithms are applied to the milk production of Holstein Friesian cows milked by an automated milking system using the dairy records and disease events. This includes 1493 cows with clinical Mastitis and 2387 healthy cows. The six models were evaluated based on five performance metrics criteria: accuracy, precision, recall, F1-score, and area under the curve (AUC). The accuracy rate ranged from 62% to 74%. The AUC is used to choose the best model. The Decision Tree algorithm and Gaussian Naïve Bayes scored the highest AUC of 71%. However, the Decision Tree algorithm is more stable with respect to other metrics (73% for accuracy and 64% for Precision, Recall, and F1-score). Hence, it can be considered the best predictive CM model with moderate accuracy. Out of the 15 input features, days in milk, age of the animal, lactation order, 305 days mature herd equivalent, and average daily milk yield were the only important features shared in establishing the Decision Tree model. [ABSTRACT FROM AUTHOR]

Simple Summary: Here, we investigated the associations of delayed milk ejection with the average milk flow rate, milking duration, and duration in a low milk flow rate in cows in a large dairy herd with suboptimal premilking stimulation. Our second aim was to study the association between peak lactation milk yield and delayed milk ejection. We found that delayed milk ejection was associated with a decreased average milk flow rate, increased milking duration, and increased duration in a low milk flow rate. Further, we found an association between peak lactation milk yield and delayed milk ejection such that cows with lower peak lactation milk yield had greater odds of delayed milk ejection. Our data suggest that delayed milk ejection has a negative impact on milking performance, and that peak lactation milk yield may serve as a proxy for the risk of delayed milk ejection. Future research is warranted to test if delayed milk ejection can be mitigated through, for example, a modified milking routine regimen, and if such a modified regimen has the potential to improve the milking performance in dairy cows. The primary objective was to investigate the association between delayed milk ejection (DME) and the average milk flow rate, milking unit-on time, and duration in a low milk flow rate in Holstein dairy cows in a large dairy herd with suboptimal premilking teat stimulation. Our second objective was to study the association between peak lactation milk yield and the occurrence of DME. This longitudinal field study was conducted at a 4300-cow dairy farm with a thrice-daily milking schedule over a 1-week period. We analyzed data from 61,677 cow milking observations from 2937 cows. Delayed milk ejection was defined as present if the 30–60 s milk flow rate was ≤3.1 kg/min. The mean average milk flow rate (MAMF, kg/min), mean milking unit-on time (MMUT, s), and mean duration of a low milk flow rate (MLMF, s) were calculated as the mean values from the 21 milking observations. General linear multivariable models revealed associations of DME with MAMF, MMUT, and MLMF. A multivariable ordinal logistic regression model revealed an association between peak lactation milk yield and DME. Cows with lower peak lactation milk yield had greater odds of exhibiting a higher frequency level of DME. The observed associations between DME and milking performance indices suggest that DME can negatively affect milking and parlor efficiency. Peak lactation milk yield may serve as a proxy to estimate cows' risk of recurrent DME. Future research is warranted to test if alleviating DME through, for example, a modified milking routine influences the milking performance indices described herein. [ABSTRACT FROM AUTHOR]

Simple Summary: Milk is one of the most globally produced foods with a growing trend, so its environmental performance needs to be studied. This research assessed the environmental impact of cow milk according to herds' performance through climate change and photochemical ozone creation potential indicators. The quality of feeds was also addressed. Under our conditions, knowing the nutritional characteristics of diets fed to animals as an alternative to tabulated values reduces the estimated impacts of considered indicators. An estimate of the environmental impact of dairy farms in Northern Italy producing milk for hard cheese (protected designation of origin) has been obtained through a comprehensive life cycle assessment. The estimate focused on climate change (CC) and photochemical ozone creation potential (POCP) indicators, which were evaluated according to the Intergovernmental Panel on Climate Change (IPCC) guidelines and interpreted with the aid of the feeds' composition evaluated using near-infrared reflectance spectroscopy (Foss NIR-System 5000) as well as with a diet evaluation according to the NRC (National Research Council) or the CNCPS (Cornell Net Carbohydrate and Protein System) nutrient requirement modeling. Herds were classified into high-, mid-, and low-performing based on the daily milk yield per cow. A lower impact on indicators was observed as herd performance increased. The high-performing herds had a lower contribution from enteric fermentation (6.30 × 10−1 kgCO2-eq), and the more milk that they produced allowed for a differentiation of CC from land use and transformation (2.39 × 10−1 kgCO2-eq), compared to low-performing herds (3.66 × 10−1 kgCO2-eq). Compared to the IPCC approach, the CC and POCP indicator estimates were reduced when addressing the feed's quality, particularly in mid- and high-performing herds. The results could be helpful in the dairy sector as they provide an insight into how diet quality affects the environmental impact of milk. [ABSTRACT FROM AUTHOR]

Objective: This study was conducted to evaluate the effect of offering (OSF) or not (NSF) supplemental feed at milking on temperament (TS), udder hygiene (UHS) and body condition (BCS) scores, and milk yield per milking (MYM), milk quality traits, and profitability of primiparous Anatolian buffalo cows at 90 days of lactation confined in closed-tied (CB) or semi-open free-stall (OB) barns. Methods: In Experiment I, 108 cows were selected to encompass four treatments (OBOSF, OB-NSF, CB-OSF, and CB-NSF) of 27 cows, considering barn type (OB and CB) and supplementary feed (OSF and NSF) at milking. In Experiment II, 60 OB cows were selected to encompass one of five groups of 12 cows each: i) no supplemental feed (CON), ii) commercial concentrate (CC), iii) CC + corn silage (CCS), iv) CCS + alfalfa hay (CSA), or v) CC + ryegrass silage (CRS) at milking. Results: The TS and UHS of the OB and OSF cows were lower (better) than those of the CB and NSF cows, respectively. The OSF increased milk protein, lactose, and solids-not-fat but decreased milk freezing point and electrical conductivity compared with the NSF. The MYM and milk fat of the OB-OSF cows were higher than those of the OB-NSF and CBNSF cows. The TS and UHS of the cows negatively correlated with MYM and some milk chemicals (fat, protein, and solids-not-fat), but BCS correlated positively. The TS and milk electrical conductivity of the CCS, CSA, and CRS cows were lower than those of the CON and CC cows, but BCS, MYM, and milk fat were higher. Partial budget analysis identified a higher net profit for supplemental feed-offered groups (OB-OSF, CCS, CSA, and CRS). Conclusion: Offering roughage with concentrates at milking for indoor primiparous buffalo cows is more conducive to well-being, milk yield, milk quality, and economy. [ABSTRACT FROM AUTHOR]

We evaluate the potential economic impact of using high-oleic soybeans (HOS) in dairy rations based on a synthesis of results from 5 prior feeding trials. Milk income less feed costs (MILFC) per cow per day is calculated based on assumed increases in milkfat production and increased cost of rations including HOS. The effects of changes in MILFC are evaluated for herds with different numbers of milking cows, and the total volume of HOS required to support different proportions of US dairy cows is calculated. A dynamic supply-chain model assesses the potential market impacts of increases in butterfat supply. The increase in milkfat from the substitution of 5% of ration dry matter with whole HOS (1.4 kg/cow per day) has the potential to increase MILFC by up to $0.27/cow per day or increase the average value of milk by $0.29/45.4 kg for a cow producing 41 kg/d. Changes in MILFC are highly correlated with the price of butter but were positive for butter prices observed from January 2014 to September 2020. The effects of HOS on MILFC suggest the potential for increases in farm profitability of $33,000/yr for a dairy feeding 500 milking cows. Scaled-up use of HOS by US dairy farmers would increase butterfat supplies and lower the butterfat price to a small extent, but these aggregated effects do not offset the positive effects of MILFC at the farm level. [ABSTRACT FROM AUTHOR]

Jujube has various functional properties and is a promising source of bioactive compounds and flavors. This study investigated the functional properties and flavor characteristics of milk from cows supplemented with jujube powder (JP). Here, milk volatile profiles and taste properties were analyzed by using an electronic nose and headspace solid-phase microextraction GC–MS. Compared with the control group, the total antioxidant capacity, 2,2′-azino-bis-3-ethylbenzothiazoline-6-sulphonic free-radical-scavenging activity, lactoferrin, and IgG levels increased significantly in the JP group. Volatile flavor analysis indicated that ketone levels increased, acid abundance decreased, and toluene and dimethyl sulfone significantly increased in the JP group. Taste-profile analyses demonstrated that jujube supplementation altered the taste of the milk. In summary, dietary JP supplementation affects the volatile flavor composition and aroma of milk, as well as the bioactive components and antioxidant properties. These findings enhance our understanding of milk production using direct dietary supplementation to produce sustainable dairy products. [ABSTRACT FROM AUTHOR]

Simple Summary: This study addresses ischemic, i.e., oxygen-deprivation-induced, teat necrosis in dairy cows, a disease that causes significant economic and animal welfare problems in the dairy industry. The aim was to investigate the clinical presentation, possible risk factors, and microbial involvement in teat necrosis. The necrosis can be recognized by an initial reddish-bloody change from the teat to the base of the udder and the eventual development of dead, dark areas and even loss of the teat, usually caused by licking by the cow. In addition to the visual change, severe itching is a particularly noticeable clinical sign. The researchers analyzed milk and swab samples from affected cows and collected questionnaires from farmers and veterinarians. The study revealed that ischemic teat necrosis predominantly affects first- and early-lactation heifers and identified infectious pathogens such as Treponema spp. and Staphylococcus aureus as well as non-infectious factors such as pre-damaged, chapped, dry, or thickened teats and certain milking factors as contributors. The results suggest that the necrosis has a multifactorial cause, combining both infectious and non-infectious factors. Understanding these factors is critical to the development of prevention and treatment strategies that aim to reduce the economic impact and improve animal welfare. Ischemic teat necrosis (ITN) is a growing problem in the dairy industry characterized by teat lesions, necrosis, pruritus and automutilation. Despite the economic and welfare consequences, there is no treatment, and the etiology of the disease remains poorly understood. The aim of this study was to investigate ITN by analyzing its clinical presentation, potential risk factors and microbial involvement. Methods included collection of milk and swab samples from affected cows over a period of one-and-a-half years and completion of questionnaires by veterinarians and farmers. Microbial testing included PCR testing for Treponema spp. and cultural testing by anaerobic and aerobic incubation on blood agar. The results showed a high and significant prevalence of Treponema spp. and Staphylococcus aureus in affected teats compared to non-ITN-affected control teats, indicating their potential role in the development of ITN. Other factors such as edema and milking practices also appear to contribute to the tissue damage. First-lactation and early-lactation heifers are particularly at risk. In conclusion, ITN appears to have a multifactorial etiology with both infectious and non-infectious factors playing a role. Further research is needed to better understand the complex interplay of these factors and to develop effective prevention and management strategies. [ABSTRACT FROM AUTHOR]

Retrospective evaluation of udder recovery following treatment of the inflamed quarter with acoustic pulse technology (APT) of cows with subclinical mastitis was done on 4 Israeli commercial dairy farms. Here, we evaluated the APT treatment as a tool to manage subclinical mastitis and its economic consequences in commercial farms. Recovery of the infected glands following APT treatment was compared to the customary no-treatment (NT) for cows with subclinical mastitis. Over 2 years, 467 cows with subclinical mastitis were identified. Subclinical mastitis was defined by elevated somatic cell count (SCC; >1 × 106 cells/mL) in the monthly test-day milk sample; 222 cows were treated with APT and 245 cows were not treated and served as control. Differences between treatment groups in culling, milk quality, milk yield and bacterial elimination were analyzed. After treatment, cure from bacteria was calculated only for cows with pre-isolated bacteria. The percentage of sampled cows determined as cured (no bacterial finding) in the NT group was 32.7% (35/107) (30.9% Gram negative; 32.4% Gram positive) and in the APT-treated group, 83.9% (42/55) (89.4% Gram negative; 80.6% Gram positive). Culling rate due to mastitis was significantly lower (>90%) in the APT-treated vs. NT group. Recovery was 66.0% in the APT group compared to 11.5% in the NT group at 90 d post-treatment. Average milk volume per cow in the APT-treated group was 16.1% higher compared to NT cows. Based on the study, savings incurred by using APT to treat only subclinical cows per 100-cow herd can total $15,106/y, or $309 per treated subclinically infected cow. [ABSTRACT FROM AUTHOR]

This study leveraged a growing dataset of producer-recorded phenotypes for mastitis, reproductive diseases (metritis and retained placenta), and metabolic diseases (ketosis, milk fever, and displaced abomasum) to investigate the potential presence of inbreeding depression for these disease traits. Phenotypic, pedigree, and genomic information were obtained for 354,043 and 68,292 US Holstein and Jersey cows, respectively. Total inbreeding coefficients were calculated using both pedigree and genomic information; the latter included inbreeding estimates obtained using a genomic relationship matrix and runs of homozygosity. We also generated inbreeding coefficients based on the generational inbreeding for recent and old pedigree inbreeding, for different run-of-homozygosity length classes, and for recent and old homozygous-by-descent segment-based inbreeding. Estimates on the liability scale revealed significant evidence of inbreeding depression for reproductive-disease traits, with an increase in total pedigree and genomic inbreeding showing a notable effect for recent inbreeding. However, we found inconsistent evidence for inbreeding depression for mastitis or any metabolic diseases. Notably, in Holsteins, the probability of developing displaced abomasum decreased with inbreeding, particularly for older inbreeding. Estimates of disease probability for cows with low, average, and high inbreeding levels did not significantly differ across any inbreeding coefficient and trait combination, indicating that although inbreeding may affect disease incidence, it likely plays a smaller role compared with management and environmental factors. [ABSTRACT FROM AUTHOR]

Simple Summary: This present research work was designed for the validation of some SNPs of casein in Bos indicus (Sahiwal breed), previously reported for their association with milk yield in Bos taurus. This study indicated that these SNPs of casein genes (rs43703010, rs109500451, rs110079521, rs110323127, rs43703015, rs43703016 and rs43703017) significantly affected milk yield in Bos indicus of the Sahiwal breed. Animals with the genotype GG, having the SNP α S1 and aA S2 casein genes (rs43703010 and rs109500451), show high milk yield. For the SNPs of the κ casein genes (CSN3) (rs110079521, rs43703016 and rs43703017), animals with genotype AA were high milk producers. For the other two SNPs of the CSN3 gene (rs110323127 and rs43703015), animals with genotypes GG and CC produced a high milk yield. In light of these results, animal breeders may be able to make a more precise selection of the animals of Bos indicus (Sahiwal breed) with high milk yield. The aim of the present study was the validation of the already reported Bos taurus SNPs in the Sahiwal breed. A total of nine SNPs of the casein gene were studied. Out of nine, seven Bos taurus SNPs of casein protein genes were found to be significantly associated with milk productivity traits. The genomic DNA was extracted from the mammary alveolar endothelial cells of a flock of 80 purebred Sahiwal lactating dams available at Khizrabad Farm near Sargodha. New allele-specific primers were designed from the NCBI annotated sequence database of Bos taurus to obtain 100 nt-long PCR products. Each dam was tested separately for all the SNPs investigated. Animals with genotype GG for the SNPs rs43703010, rs10500451, and 110323127, respectively, exhibited high milk yield. Similarly, animals with genotype AA for the SNPs rs11079521, rs43703016, and rs43703017 showed high milk yield consistently. For the SNP rs43703015, animals with genotype CC showed high milk productivity. These above-mentioned SNPs have previously been reported to significantly up-regulate casein protein contents in Bos taurus. Our results indicated SNPs that significantly affect the milk protein contents may also significantly increase per capita milk yield. These finding suggest that the above-mentioned reported SNPs can also be used as genetic markers of milk productivity in Sahiwal cattle. [ABSTRACT FROM AUTHOR]

Simple Summary: Bovine paratuberculosis, a chronic infectious disease, is a significant concern in ruminants and wild animals, often leading to severe health issues and economic losses. In China, it is a prevalent yet underreported bovine disease. Our study aimed to fill this gap by investigating the prevalence of bovine paratuberculosis in Hubei Province. We also evaluated milk and blood antibody tests for paratuberculosis, which showed a high overall agreement of 92.0%. Using the milk test, we found that the highest lacto-prevalence at the individual level reached 22.9%, with a farm-level prevalence of 92.3% in January and 84.6% in April 2018. The total incidence risk of all farms was 6% per 3 months. Notably, large-scale farms had a significantly lower prevalence and incidence than small-scale farms. These findings underscore the need for robust prevention and control measures. Furthermore, our study underscores the significant impact of bovine paratuberculosis on milk quality, with a notable increase in somatic cell counts. These findings provide crucial insights into the prevalence and incidence risk of paratuberculosis in China, serving as a vital foundation for advocating for its prevention and control. Mycobacterium avium subsp. paratuberculosis (MAP) is responsible for the persistent infectious illness known as bovine paratuberculosis, which is one of the most easily overlooked diseases in China amid a lack of epidemiological data. In this study, we evaluated the agreement of milk and blood antibody tests for paratuberculosis and showed an overall agreement of 92.0%, with a 95.0% negative coincidence rate and a 78.6% positive coincidence rate. The milk test was then used to examine the prevalence and incidence of dairy cows in Hubei Province, China. We found that, at the individual level, the highest lacto-prevalence reached up to 22.9%; the farm-level prevalence was as high as 92.3% (12/13) and 84.6% (11/13) in January and April 2018, respectively. The total incidence risk of all farms was 6% per three months. We also found that large-scale farms had a significantly lower prevalence and incidence than small-scale farms. Finally, the correlation between paratuberculosis and milk quality was evaluated, and we confirmed that MAP can significantly alter milk quality and raise somatic cell counts in the milk. This study provides valuable information for assessing the prevalence and incidence risk of paratuberculosis in China. It further provides an essential basis for calling for the prevention and control of paratuberculosis in China. [ABSTRACT FROM AUTHOR]

Simple Summary: Currently, smart devices for cows on the market are mainly leg rings and collars, but the behavioral data provided by these devices do not well reflect the real behavior of cows. Therefore, a cow-behavior-detection device based on a wearable device is proposed. It is a set of equipment that collects data on the daily behavior (eating, rumination, other behavior) of cows, which can help people monitor the health status of cows more accurately. This paper proposes for the first time an electronic device worn on the nose of a cow to record real-time behavioral data of the cow. Through these data, the daily behavior of cows can be analyzed, such as the time spent eating and ruminating that day, the number of rumination chews, etc., which can better help farm managers understand the health status of cows, reduce the occurrence of diseases, and thereby improve the overall health of the cows' welfare. The wearing position of the device has no adverse effects on the normal life of the cows and is suitable for long-term wearing. This equipment helps improve the welfare of dairy cows and has far-reaching value for the dairy farming industry. This study introduces a novel device designed to monitor dairy cow behavior, with a particular focus on feeding, rumination, and other behaviors. This study investigates the association between the cow behaviors and acceleration data collected using a three-axis, nose-mounted accelerometer, as well as the feasibility of improving the behavioral classification accuracy through machine learning. A total of 11 cows were used. We utilized three-axis acceleration sensors that were fixed to the cow's nose, and these devices provided detailed and unique data corresponding to their activity; in particular, a recorder was installed on each nasal device to obtain acceleration data, which were then used to calculate activity levels and changes. In addition, we visually observed the behavior of the cattle. The characteristic acceleration values during feeding, rumination, and other behavior were recorded; there were significant differences in the activity levels and changes between different behaviors. The results indicated that the nose ring device had the potential to accurately differentiate between eating and rumination behaviors, thus providing an effective method for the early detection of health problems and cattle management. The eating, rumination, and other behaviors of cows were classified with high accuracy using the machine learning technique, which can be used to calculate the activity levels and changes in cattle based on the data obtained from the nose-mounted, three-axis accelerometer. [ABSTRACT FROM AUTHOR]

The current study was conducted on the inhabitants living in the area adjacent to the Hudiara drain using bore water and vegetables adjacent to the Hudiara drain. Toxic heavy metals badly affect human health because of industrial environmental contamination. Particularly hundreds of millions of individuals globally have faced the consequences of consuming water and food tainted with pollutants. Concentrations of heavy metals in human blood were elevated in Hudiara drainings in Lahore city, Pakistan, due to highly polluted industrial effluents. The study determined the health effects of high levels of heavy metals (Cd, Cu, Zn, Fe, Pb, Ni, Hg, Cr) on residents of the Hudiara draining area, including serum MDA, 8-Isoprostane, 8-hydroxyguanosine, and creatinine levels. An absorption spectrophotometer was used to determine heavy metals in wate water, drinking water, soil, plants and human beings blood sampleas and ELISA kits were used to assess the level of 8-hydroxyguanosine, MDA, 8-Isoprostane in plasma serum creatinine level. Waste water samples, irrigation water samples, drinking water samples, Soil samples, Plants samples and blood specimens of adult of different weights and ages were collected from the polluted area of the Hudiara drain (Laloo and Mohanwal), and control samples were obtained from the unpolluted site Sheiikhpura, 60 km away from the site. Toxic heavy metals in blood damage the cell membrane and DNA structures, increasing the 8-hydroxyguanosine, MDA, creatinine, and 8-Isoprostane. Toxic metals contaminated bore water and vegetables, resulting in increased levels of creatinine, MDA, Isoprostane, and 8-hydroxy-2-guanosine in the blood of inhabitants from the adjacent area Hudiara drain compared to the control group. In addition,. This study also investigated heavy metal concentrations in meat and milk samples from buffaloes, cows, and goats. In meat, cow samples showed the highest Cd, Cu, Fe and Mn concentrations. In milk also, cows exhibited elevated Cu and Fe levels compared to goats. The results highlight species-specific variations in heavy metal accumulation, emphasizing the need for targeted monitoring to address potential health risks. The significant difference between the two groups i.e., the control group and the affected group, in all traits of the respondents (weight, age, heavy metal values MDA, 8-Isoprostane, 8-hydroxyguaniosine, and serum creatinine level). Pearson's correlation coefficient was calculated. The study has shown that the level of serum MDA, 8-Isoprostane, 8-hydroxyguaniosine, or creatinine has not significantly correlated with age, so it is independent of age. This study has proved that in Pakistan, the selected area of Lahore in the villages of Laloo and Mohanwal, excess of heavy metals in the human body damages the DNA and increases the level of 8-Isoprostane, MDA, creatinine, and 8-hydroxyguaniosine. As a result, National and international cooperation must take major steps to control exposure to heavy metals. [ABSTRACT FROM AUTHOR]

A cross-sectional study was conducted to estimate the prevalence of intramammary infection (IMI) associated bacteria and to identify risk factors for pathogen group-specific IMI in water buffalo in Bangladesh. A California Mastitis Test (CMT) and bacteriological cultures were performed on 1,374 quarter milk samples collected from 763 water buffalo from 244 buffalo farms in nine districts in Bangladesh. Quarter, buffalo, and farm-related data were obtained through questionnaires and visual observations. A total of 618 quarter samples were found to be culture positive. Non-aureus staphylococci were the predominant IMI-associated bacterial species, and Staphylococcus (S.) chromogenes, S. hyicus, and S. epidermidis were the most common bacteria found. The proportion of non-aureus staphylococci or Mammaliicoccus sciuri (NASM), S. aureus, and other bacterial species identified in the buffalo quarter samples varied between buffalo farms. Therefore, different management practices, buffalo breeding factors, and nutrition were considered and further analyzed when estimating the IMI odds ratio (OR). The odds of IMI by any pathogen (OR: 1.8) or by NASM (OR: 2.2) was high in buffalo herds with poor milking hygiene. Poor cleanliness of the hind quarters had a high odds of IMI caused by any pathogen (OR: 2.0) or NASM (OR: 1.9). Twice daily milking (OR: 3.1) and farms with buffalo purchased from another herd (OR: 2.0) were associated with IMI by any pathogen. Asymmetrical udders were associated with IMI-caused by any bacteria (OR: 1.7). A poor body condition score showed higher odds of IMI by any pathogen (OR: 1.4) or by NASM (OR: 1.7). This study shows that the prevalence of IMI in water buffalo was high and varied between farms. In accordance with the literature, our data highlight that IMI can be partly controlled through better farm management, primarily by improving hygiene, milking management, breeding, and nutrition. [ABSTRACT FROM AUTHOR]

Various management practices can influence milk quality traits in dairy cattle. As an example, an increasing investment in automatic milking system to substitute milking parlors has been observed in the last 2 decades in dairy farms which could have affected certain bulk milk quality traits. What is more, milking practices can also affect certain milk parameters; as an example, teat disinfectants containing I are used in commercial farms where pre- or postdipping is performed, leading to presence of some I in the bulk milk. However, this trace mineral is also supplied in cows' diet to fulfill their nutritional requirements, partly contributing to the milk I final concentration. Therefore, the aim of this study was to evaluate the sources of variation of milk I along with other traditional milk quality traits. A total of 91 dairy farms in northeastern Italy were enrolled in the study. In each farm, diet and bulk milk samples were collected on the same day for chemical analysis. Concentration of I, in particular, was determined in both milk and feed with gold standard. Pearson correlations were calculated among the traits available for milk and diet, and a general linear model was used to test significance of fixed effects (feeding system, milking system, farming system, herd size, herd stage of lactation, and sampling month) on milk quality traits including the I concentration. In the case of milk I, diet I and presence of I-based predipping and postdipping teat disinfect application were also tested as fixed effects. Results showed a positive linear correlation between milk and diet I content (correlation coefficient [r] = 0.78). Although milk I was also positively correlated with lactose content (r = 0.25), dietary I was not correlated with other milk traits. Milk I content was significantly affected by dietary I, I-based predipping teat disinfectant application, and herd composition. Compared with conventional farms, organic farms showed lower protein content and greater somatic cell score (SCS) but similar milk I. Milking system significantly affected only lactose content and SCS of milk. Sampling month was only significant for milk urea nitrogen and herd composition, feeding system, herd size, and herd average days in milk did not modify milk gross composition and SCS. In conclusion, dietary supply of I is the main factor affecting milk I concentration and findings suggest that I level in milk can be naturally improved in dairy cows by modulating the I content in the diet administered. However, further research is needed to evaluate the effect of I-based sanitizers on milk I. [ABSTRACT FROM AUTHOR]

BACKGROUND: Lucerne protein extract is a novel high‐quality protein source with excellent amino acid (AA) composition of interest for human consumption. In this study, protein from screw‐pressed lucerne juice was extracted by different precipitation methods to evaluate the effect on the chemical composition and nutritional quality of the extracted protein. Methods based on heat, acidification or fermentation were used for protein precipitation, and the nutritional value of protein was evaluated in a rat digestibility trial. RESULTS: Heat precipitation at 85 °C produced a protein product with a crude protein (CP) content of 589 g kg−1 dry matter (DM), a balanced AA composition and a high standardized nitrogen (N) digestibility (82.8%). Precipitation by acidification, at a lower temperature (60 °C) or by fermentation, resulted in lower CP content (425–488 g kg−1 DM). Nitrogen digestibility for the pH‐adjusted precipitate was equal to the 85 °C heat‐precipitated protein, while the fermented and 60 °C precipitated proteins showed lower N digestibility (76.5% and 78.6%, respectively). By applying a two‐step heat precipitation method (60 °C followed by 80 °C), a protein content of 712 g kg−1 DM and an N digestibility of 93.6% was reached, which are comparable to high‐quality animal‐based protein sources such as milk, whey, casein, and eggs, covering the AA requirements for children >6 months. CONCLUSION: High‐quality protein can be extracted from lucerne, but the future focus should be on increased yield as the current low yields of the refined product will challenge the environmental and economic sustainability of production. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Intestinal epithelial cells (IECs) play crucial roles in forming an essential barrier, providing host defense against pathogens and regulating nutrients absorption. Milk-derived extracellular vesicles (EVs) within its miRNAs are capable of modulating the recipient cell function. However, the differences between colostrum and mature milk EVs and their biological function in attenuating intestinal epithelial cell injury remain poorly understood. Thus, we carried out the present study to characterize the difference between colostrum and mature milk-derived miRNA of EVs and the effect of colostrum and mature milk EVs on the proliferation, apoptosis, proinflammatory cytokines and intestinal epithelial barrier related genes in IEC-6 induced by LPS. Differential expression of 329 miRNAs was identified between colostrum and mature milk EVs, with 185 miRNAs being downregulated and 144 upregulated. In addition, colostrum contains a greater number and protein concentration of EVs than mature milk. Furthermore, compared to control, EVs derived from colostrum significantly inhibited the expression of apoptosis- (Bax, p53, and caspase-3) and proinflammatory-related genes (TNFα, IL6, and IL1β). EVs derived from mature milk did not affect expression of apoptosis-related genes (Bax, p53, bcl2, and caspase-3). The EVs derived from mature milk significantly inhibited the expression of proinflammatory-related genes (TNFα and IL6). Western blot analysis also indicated that colostrum and mature milk EVs significantly decreased the apoptosis of IEC-6 cells. The EdU assay results showed that colostrum and mature milk EVs significantly increased the proliferation of IEC-6 cells. The expression of intestinal barrier-related genes (TJP1, CLDN1, OCLN, CDX2, MUC2, and IGF1R) was significantly promoted in IEC-6 cells after colostrum and mature milk EVs addition. Importantly, colostrum and mature milk EVs significantly relieved the LPS-induced inhibition of proliferation and intestinal barrier-related genes expression and attenuated apoptosis and proinflammatory responses induced by LPS in IEC-6 cells. Flow cytometry and Western blot analysis also indicated that colostrum and mature milk EVs significantly affect the apoptosis of IEC-6 cells induced by LPS. The results also indicated that EVs derived from colostrum had better effects on inhibiting the apoptosis- and proinflammatory cytokines-related genes expression. However, the EVs derived from mature milk exhibited beneficial effects on intestinal epithelial barrier protection. The present study will provide a better understanding of the role of EVs derived from colostrum and milk in dairy cows with different responses in the regulation of intestinal cells function, and also presents new evidence for the change of EVs cargos during various stages of lactation. [ABSTRACT FROM AUTHOR]