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One of the best-studied responses of cognitive evoked potentials is a so-called 'P300', the late positive wave complex that occurs about 300-500 ms after the stimulus. It is obtained when the subject's attention is focused on a signal that is rare, especially if the signal has a motivational or emotional meaning. In the study of P300 potential, we followed the variations of potential amplitude and latency, so the objective was to examine whether there is a difference in Fz and Cz amplitudes of auditory induced cognitive evoked P300 potential depending on the performance of oddball tasks, both in male and female subjects. The study included 60 subjects (30 female respondents and 30 male respondents). P300 potential is induced by the auditory 'oddball' paradigm with 80% of non-target and 20% of target stimuli that are presented to the patient through headphones. The target tones are high tones of 2000 Hz. The standard, 1000 Hz tones the respondent should ignore but when he hears the target tones the respondent should press the button on the special handle. The value of Fz and Cz amplitudes both in male and female subjects obtained in the classical 'oddball' paradigm when the subject reacted to the signal by pressing the key with the dominant (right) arm were statistically significantly lower (p>0,05) than the values of Fz and Cz amplitudes obtained when the key was pressed by the non-dominant hand. Based on this experiment it can be concluded that both in male and female subjects the performance of oddball tasks does not affect the amplitude of P300 cognitive evoked potentials.

Zoran Milanović,1 Saša Pantelić,1 Nebojša Trajković,1 Bojan Jorgić,1 Goran Sporiš,2 Milovan Bratić1 1Faculty of Sport and Physical Education, University of Niš, Niš, Serbia; 2Faculty of Kinesiology, University of Zagreb, Zagreb, Croatia Abstract: The purpose of this study was to determine the test–retest reliability of the International Physical Activity Questionnaire (IPAQ) for older adults in Serbia. Six hundred and sixty older adults (352 men, 53%; 308 women, 47%; mean age 67.65±5.76 years) participated in the study. To examine test–retest reliability, the participants were asked to complete the IPAQ on two occasions 2 weeks apart. Moderate reliability was observed between the repeated IPAQ, with intraclass correlation coefficients ranging from 0.53 to 0.91. The least reliability was established in leisure time activity (0.53) and the most reliability in the transport domain (0.91). Men and women had similar intraclass correlation coefficients for total physical activity (0.71 versus 0.74, respectively), while the biggest difference was obtained for housework in men (0.68) and in women (0.90). Our study shows that the long version of the IPAQ is a reliable instrument for assessing physical activity levels in older adults and that it may be useful for generating internationally comparable data. Keywords: questionnaire, elderly, IPAQ, physical activity

The role of endothelin in cardiovascular physiology and pathophysiology has been undeniable ever since its discovery. Endothelins in the heart are important for the development, growth and remodeling, as well as for the control of contractility and rhythm. Cardiac endothelial cells in the endocardium and myocardial capillaries represent the main source of endothelin, and cardiomyocytes are their primary targets. Endothelin-1 is one of the most potent substances with a positive inotropic effect known to man; subsequently endothelin plays a key role in the cardiac endothelial-myocardial interaction. The endothelins are a family of 21 amino acid peptidesof which there are three distinct isoforms endothelin-1, endothelin-2 and endothelin-3. Their effects are achieved by the activation of endothelin receptors, ETA and ETB, which belonging to the family of G protein-linked receptors. ETA and ETB receptors are densely distributed in cardiomyocytes, the cells of the cardiovascular system, coronary vascular and endocardial endothelial cells. Under physiological conditions, endothelin is synthesized in endothelial cells, while under pathophysiological conditions in the large number of non-endothelial cells of the heart as well. Endothelin-1 has positively inotropic and chronotropic effects. The administration of ET-1 causes coronary vasoconstriction, leads to myocardial ischemia and a lethal ventricular arrhythmia. In the acute myocardial infarction, ET-1 increases myocardial necrosis and arrhythmia but has a favorable effect on heart recovery after a myocardial infarction at an early stage of cardiac remodeling. ET-1 reverses acidosis-induced negative lusitropic and inotropic effects without the increase of intracellular calcium. Endothelin can resist the arrhythmogenic effects of catecholamines. Thus, low concentrations of endothelin have a protective effect on the heart. Primary indications for the administration of endothelin antagonists are heart failure, pulmonary hypertension and resistant arterial hypertension. Careful and well-designed clinical studies are needed to verify the therapeutic potential of new types of drugs in cardiovascular medicine.

Zoran Milanovic,1 Saša Pantelic,1 Nebojša Trajkovic,1 Goran Sporiš,2 Radmila Kostic,1 Nic James31Faculty of Sport and Physical Education, University of Niš, Niš, Serbia; 2Faculty of Kinesiology, University of Zagreb, Zagreb, Croatia; 3London Sport Institute, Middlesex University, London, UKAim: To determine differences in physical activity level and functional fitness between young elderly (60–69 years) and old elderly (70–80 years) people with the hypothesis that an age-related decline would be found.Methods: A total of 1288 participants’ level of physical activity was evaluated using the International Physical Activity Questionnaire: 594 were male (mean ± standard deviation: body height 175.62 ± 9.78 cm; body weight 82.26 ± 31.33 kg) and 694 female (mean ± standard deviation: body height 165.17 ± 23.12 cm; body weight 69.74 ± 12.44 kg). Functional fitness was also estimated using the Senior Fitness Test: back scratch, chair sit and reach, 8-foot up and go, chair stand up for 30 seconds, arm curl, and 2-minute step test.Results: Significant differences (P < 0.05) were found for all Senior Fitness tests between young elderly (60–69 years) and old elderly (70–80) men. Similar results were found for the women, except no significant differences were found for the chair sit and reach and the 2-minute step test. From the viewpoint of energy consumption estimated by the International Physical Activity Questionnaire, moderate physical activity is dominant. In addition, with aging, among men and women older than 60 years, the value of the Metabolic Equivalent of Task in total physical activity significantly reduces (P < 0.05).Conclusions: This study found that the reduction in physical activity level and functional fitness was equal for both men and women and was due to the aging process. These differences between young and old elderly people were due to the reduction of muscle strength in both upper and lower limbs and changes in body-fat percentage, flexibility, agility, and endurance.Keywords: older adult, functional capacity, strength, agingCorrigendum for this paper has been published

Milanović Z, Pantelić S, Trajković N, et al. Clin Interv Aging. 2013;8:549–556.On page 551, Table 1, the Note should have read:Notes: *P

It was reported that novel O,O′-diethyl-(S, S)-ethylenediamine-N,N′-di-2-(3-cyclohexyl) propanoate dihydrochloride (DE-EDCP) displayed in vitro antiproliferative activity on several human and mouse cancer cell lines, which was comparable to that of the prototypical anticancer drug cisplatin. In order to reveal its toxicity profile, acute and repeated-dose toxicity studies were performed in Naval Medical Research Institute (NMRI) Han mice. The intravenous LD50 values of DE-EDCP were found to be 95.3 and 101.3 mg/kg body weight in female and male mice, respectively. In the subacute toxicity study, DE-EDCP was administered intravenously at the doses of 15, 25, and 40 mg/kg/day for a period of 28 days. There were no adverse effects on general condition, growth, feed and water consumption, and hematological parameters. There was a significant increase in urea and alanine aminotransferase in female mice and aspartate aminotransferase and alkaline phosphatase in both genders in 40 mg/kg/day dose-treated group. The histopathological changes confined to the liver and kidney, but in other organs were not found. Satellite group revealed that changes in the kidney and liver were less pronounced, suggesting their reversibility. Interactions with DNA could also be of importance for understanding DE-EDCP toxic side effects. Hyperchromic effect obtained with ultraviolet–visible, suggested electrostatic interactions between DE-EDCP and calf thymus DNA. The toxicity testing of DE-EDCP was conducted to predict human outcomes. [ABSTRACT FROM AUTHOR]

In the present manuscript, three different 4,7-dihydroxycoumarin derivatives were prepared and structurally characterized by crystallographic and spectroscopic techniques in combination with the B3LYP-D3BJ theoretical method. Cytotoxic and antimicrobial activities of investigated compounds were screened against different cell lines and microorganisms. HCT-116 cells were most sensitive to the 3-(1-(2-hydroxyphenyl)amino) ethylidene)-2,4-dioxochroman-7-yl acetate derivative, while the best antimicrobial activity against Bacillus subtilis ATCC 6633 was shown by 3-(1-(2-hydroxyphenyl)amino)ethylidene)-2,4-dioxochroman-7-yl acetate. The molecular docking study for all compounds with important epidermal growth factor receptors (EGFR) was performed. The results indicate that the largest contribution to the binding energy is through conventional hydrogen bonds.

The aims of this study were: 1) To determine the effects of a 12-week recreational soccer training programme and continuous endurance running on body composition of young adult men and 2) to determine which of these two programmes was more effective concerning body composition. Sixty-four participants completed the randomized controlled trial and were randomly assigned to one of three groups: a soccer training group (SOC; n=20), a running group (RUN; n=21) or a control group performing no physical training (CON; n=23). Training programmes for SOC and RUN lasted 12-week with 3 training sessions per week. Soccer sessions consisted of 60 min ordinary five-a-side, six-a-side or seven-a-side matches on a 30-45 m wide and 45-60 m long plastic grass pitch. Running sessions consisted of 60 min of continuous moderate intensity running at the same average heart rate as in SOC (~80% HRmax). All participants, regardless of group assignment, were tested for each of the following dependent variables: body weight, body height, body mass index, percent body fat, body fat mass, fat-free mass and total body water. In the SOC and RUN groups there was a significant decrease (p<0.05) in body composition parameters from pre- to post-training values for all measures with the exception of fat-free mass and total body water. Body mass index, percent body fat and body fat mass did not differ between groups at baseline, but by week 12 were significantly lower (p<0.05) in the SOC and RUN groups compared to CON. To conclude, recreational soccer training provides at least the same changes in body composition parameters as continuous running in young adult men when the training intensity is well matched. [ABSTRACT FROM AUTHOR]

Disturbances of thyroid function are often accompanied by cognitive and affective disorders. Assessment of cognitive status in the patients with subclinical hypothyroidism represents a possibility for early diagnostics of cognitive impairment and timely introduction of necessary pharmacotherapy treatment. The aim of this study was to quantify whether there are P3 event related potential (ERP) deviations as electrophysiological markers of cognitive activity in patients with subclinical hypothyroidism. P300 potentials were examined in thirty patients with subclinical hypothyroidism and in 30 healthy subjects of the control group. P300 was recorded using the classic auditory oddball paradigm, with 20% of target and 80% of non-target stimuli. The results analysis showed a significantly longer latency P300 and reduced amplitude P300 in subjects with subclinical hypothyroidism compared to euthyroid subjects. There is also a statistically significant negative correlation between the results of a mini mental state examination and the P300 latency at Fz electrode (r= -0.47, p

The aim of this study was to determine the craniofacial parameters in the population of the central part of Serbia. The research was conducted on 700 persons (360 males and 340 females), aged 18-65 years, selected randomly. The measured parameters were morphological facial height and breadth. The standard spreading caliper with scale was used for the measurement of facial parameters. There were significant differences in the facial parameters of male compared to female subjects in all observed parameters. The mean value of the morphological facial height in the study population was 116.8 mm ± 7.28, maximum facial breadth 124.12 mm ± 8.44, while the mean value of the total facial index was 93.68 ± 6.86. The total facial index was calculated according to the formula and the obtained results were analyzed statistically using the t-test. The dominant phenotype in the studied population was leptoprosopic. The data obtained in our study may be useful in anthropological research, forensics, genetic research, as well as in medical clinical practice.

Purpose: The purpose of this study was to determine the relationship between linear and change-of-direction sprinting performance with dribbling performance and Dribble Deficit in professional female handball players., Methods: Eleven professional female handball players (mean age: 21.12 ± 4.34 years; body height: 171.59 ± 4.52 cm; body weight: 66.29 ± 5.73 kg) participated in the study. Each participant completed several linear (sprint over 10, 20, and 30 m) and change-of-direction tests (slalom test, zig-zag test, 505 test), first without the ball (sprinting performance) followed by ball dribbling (dribbling performance). Dribble Deficit was calculated indirectly as the time difference between the best trial while dribbling minus the best trial without dribbling., Results: A large to very large correlation was observed between the linear sprint and dribbling performance (r = 0.53-0.78), as well as between change-of-direction sprinting performance and dribbling performance (r = 0.66-0.88). The study also showed a moderate to perfect relationship between linear dribbling performance and Dribble Deficit (r = 0.46-0.93), and a large relationship between change-of-direction dribbling performance and Dribble Deficit (r = 0.54-0.55), while the relationships between linear sprinting performance and Dribble Deficit (r = -0.51-0.21) and between change-of-direction sprinting performance and Dribble Deficit (r = -0.14-0.26) were small and non-significant., Conclusion: In summary, Dribble Deficit reflects dribbling ability independent of sprinting ability and refines its application for practical use in assessing dribbling skills in female handball players., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2025 Pavlović, Lazić, Čović, Pišot, Petronijević and Milanović.)

The aim of this study was to determine the effects of linear and change of direction high-intensity interval training (HIIT) on physical performance in elite female soccer players. Thirty elite female soccer players (age: 19.6 ± 4.6 years; height: 166.5 ± 4.8 cm; body weight: 60.5 ± 8.3 kg; BMI: 21.9 ± 2.9 kg/m 2 ) were randomly allocated to HIIT linear (HIIT LIN ) and HIIT change of direction (HIIT COD ) training groups. The HIIT LIN group performed linear running for 15, 20 or 25 s by keeping constant pace during the entire distance. In contrast, the HIIT COD group performed three changes of direction (COD) with a 180° turn during each interval run at 15, 20 or 25 s. Physical performance was assessed using sprinting, agility, vertical jumps, repeated sprint ability (RSA) and 30-15 Intermittent Fitness Test (30-15 IFT). In both groups, all physical performance measurements improved (p ≤ 0.05), except RSAbest in HIIT LIN (p = 0.45). Both interventions significantly improved speed over 10 m, 20 m, 30 m, Pro-agility, Zig-zag, RSAavg, fatigue index, maximal oxygen uptake, and velocity at 30-15 IFT, while moderate improvements were observed in countermovement jump (CMJ), CMJ with arm swing and squat jump. However, HIIT COD did not achieve superior improvements in any of the aforementioned measurements compared to HIIT LIN . Based on the obtained results, we concluded that different types of HIIT training have a positive effect on physical performance in elite female soccer players., Competing Interests: The authors declared no conflict of interest., (Copyright © Institute of Sport – National Research Instutite.)

The purpose of this study was to investigate the acute effect of low, moderate, or high doses of taurine on anaerobic and physiological performance in female rugby players. A total of 16 sub-elite female rugby athletes (21.3 ± 1.5 yr, 168.0 ± 4.9 cm, 62.1 ± 3.5 kg mean ± SD) participated in this research. Following familiarization, participants attended 4 successive tests separated by 72 h in a randomized, counter-balanced, crossover research design. Following an over night fast, participants completed a 5-min 60 Watt warm-up followed by a 30-s Wingate anaerobic test on a cycle ergometer after ingestion of either 2 g taurine (LOWTAU), 4 g (MODTAU), 6 g (HIGHTAU) or placebo (PLA) 1 h before the test. Peak (PP) and mean power (MP) along with heart rate (HR), rating of perceived exertion (RPE), capillary lactate and blood glucose were measured. LOWTAU did not affect PP, MP, HR, lactate and glucose compared to PLA ( p  > 0.05), while MODTAU improved MP and HIGHTAU improved PP and MP compared to PLA and LOWTAU ( p  < 0.05). MODTAU and HIGHTAU had little effect on HR, blood lactate and glucose ( p  > 0.05). A single dose of taurine (MODTAU or HIGHTAU) 1 h prior to competition or training would provide an ergogenic effect on subsequent power output.

Purpose: The present study aimed to investigate a) the associations between bilateral performance utilizing countermovement jump (CMJ), squat jump (SJ), speed and unilateral CMJ, isokinetic peak torque in knee extension and flexion with angular velocities of 60°/s and 180°/s and tensiomyography (TMG) parameters; b) whether the asymmetries derived from unilateral tests are associated with bilateral CMJ, SJ and speed in elite female soccer players., Methods: Thirty-five elite female soccer players (average age: 20 ± 5 years) completed CMJ, SJ, speed, isokinetic muscle strength and TMG tests., Results: Compared to the non-dominant leg, the dominant leg demonstrated greater peak torque output in both knee flexion (7.4%) and knee extension (5.6%) isokinetic tasks, as well as m. vastus medialis contraction time (7.6%), and soccer-specific agility test (4.1%). Conversely, the hamstring to quadriceps peak torque ratio at 180°/s (8.5%) was significantly greater in the non-dominant leg. The associations between CMJ, SJ and speed performance were positive and ranged from weak ( r = 0.350) to high ( r = 0.710). For speed and TMG-derived variables, correlations were negative and ranged from weak ( r = -0.345, p = 0.042, for vastus medialis contraction time) to moderate ( r = -0.530, p = 0.001, for biceps femoris contraction time). Furthermore, both bilateral CMJ and SJ negatively correlated with TMG-derived variables, ranging from weak ( r = -0.350, p = 0.039, for vastus lateralis contraction time) to moderate ( r = -0.537, p = 0.003, for rectus femoris contraction time)., Conclusion: The overall significant, albeit inconsistent, correlations between the diverse performance scores obtained highlight the necessity for a multifaceted and thorough diagnostic strategy in female soccer players., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2024 Paravlic, Abazovic, Milanović, Vučković, Spudić, Hadzic, Pajek and Vodičar.)

Background: Cerebral palsy (CP) is the most common cause of physical disability in childhood defined as a group of permanent disorders of movement. The aim of this study was to determine the effects of 12-week aquatic exercise program on gross motor function, swimming skills, and walking ability in children with cerebral palsy., Methods: Eighteen children (mean±SD age: 12.3±3 years) with cerebral palsy classified at levels I, II and III on the Gross Motor Function Classification System were allocated to one group, where the first 12 weeks were a control period while another 12 weeks were an experimental period. The participants underwent the same battery of tests focusing gross motor function, swimming skills, and walking ability on three occasions., Results: Control period was stable with no significant changes in any of measurements. After the 12-week experimental program, a statistically significant improvement was determined in gross motor function (P=0.005), swimming skills (P=0.000), walking endurance and walking velocity (P=0.000). No significant differences (P>0.05) were observed for walking efficiency., Conclusions: The 12-week aquatic exercise program (3/week, 60 minutes), combining Halliwick method, swimming and walking activities may improve the gross motor function, swimming skills, walking endurance and velocity in ambulatory children with cerebral palsy.

The objectives of this study were to investigate: 1) whether there were morning-to-evening differences in short-term maximal performance and 2) the impact of prolonged and specific warm-up on short-term maximal performance diurnal variations in young basketball players. Fifteen basketball players of both sexes (Male = 8; Female = 7; age: 14.4 ± 0.46 yr; weight: 64.7 ± 7.1 kg; height: 175.2 ± 6.6 cm; BMI: 21.1 ± 1.9 kg/m 2 ) completed the following short-term maximal performance tests: CMJ with and without arm swing, Lane Agility Drill, Zig-Zag agility test with and without the ball, Sprint 20 m with and without the ball with the passage at 5 and 10 m. All tests were performed after the 15-min standard warm-up procedure (with static stretching) and/or 25-min specific warm-up (with prolonged running and dynamic stretching) in the morning and evening. Vertical jumping tests and all change-of-direction speed tests (with and without a ball) with superior responses were achieved in the evening after standard warm-up among all participants ( p  < 0.05). In contrast, superior short-term maximal performance was observed in the morning after prolonged and specific warm-up protocol ( p  < 0.05). It was concluded that specific and prolonged warm-up protocols are suitable strategy to prevent diurnal variation in short-term maximal performance in young basketball players.

J. nigra leaf contains mixture of various pharmacologically active compounds and it is assumed that they may have the potential radioprotective effect. The purpose of this work was to predict radioprotective doses by correlating changes in organ distribution of radiopharmaceuticals with extract dose levels and rat body weight using response surface methodology (RSM) based on a second-order polynomial equation. The extract was administered daily by oral gavage to rats at dose of 6.9, 10.3, or 13.7 mg kg -1 body weight (bw) day -1 for 10 days. On the eleventh day, 0.1 ml of the one radiopharmaceutical (Na 99m TcO 4 , 99m Tc-dimercaptosuccinic acid and 99m Tc-tin colloid) was injected into the tail vein. The statistical parameters: the coefficient of determination (0.81-0.95), the coefficient of variation (3.05-11.1), the adequate precision (8.82-19.12) and the mean relative percentage deviation (± 2.3-8.2) were indicated the precision and reliability of RSM. Using RSM, the predicted daily dose of leaf extract ranging from 11.19 to 11.99 mg kg -1 bw may be considered as an optimal daily radiopotective dose for protection of organs from radiation in cases of planned radiation exposures., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

As an alternative to classical brachytherapy, intratumoral injection of radionuclide-labeled nanoparticles (nanobrachytherapy, NBT) has been investigated as a superior delivery method over an intravenous route for radionuclide therapy of solid tumors. We created superparamagnetic iron oxide nanoparticles (SPIONs) coated with meso-1,2-dimercaptosuccinic acid (DMSA) and radiolabeled with Lutetium-177 ( 177 Lu), generating 177 Lu-DMSA@SPIONs as a potential antitumor agent for nanobrachytherapy. Efficient radiolabeling of DMSA@SPIONS by 177 Lu resulted in a stable bond with minimal leakage in vitro. After an intratumoral injection to mouse colorectal CT-26 or breast 4T1 subcutaneous tumors, the nanoparticles remained well localized at the injection site for weeks, with limited leakage. The dose of 3.70 MBq/100 µg/50 µL of 177 Lu-DMSA@SPIONs applied intratumorally resulted in a high therapeutic efficacy, without signs of general toxicity. A decreased dose of 1.85 MBq/100 µg/50 µL still retained therapeutic efficacy, while an increased dose of 9.25 MBq/100 µg/50 µL did not significantly benefit the therapy. Histopathology analysis revealed that the 177 Lu-DMSA@SPIONs act within a limited range around the injection site, which explains the good therapeutic efficacy achieved by a single administration of a relatively low dose without the need for increased or repeated dosing. Overall, 177 Lu-DMSA@SPIONs are safe and potent agents suitable for intra-tumoral administration for localized tumor radionuclide therapy.

The aims of this study were to determine the relationship between vertical jumping performance, linear speed, change of direction speed (CODs) time and the COD deficit (CODD) in adolescent basketball players and to analyze the CODD between faster and slower players based on linear speed performance. Thirty-eight male basketball players (age: 15.47 ± 0.51 years; body height: 185.19 ± 5.67 cm; body mass: 71.87 ± 7.29 kg) completed countermovement jumps (CMJ) with and without an arm swing, squat jumps (SJs), linear sprints at 20 m with split times at 5 and 10 m, the pro-agility test and the zig-zag tests. Furthermore, the CODD was calculated as the difference between the 20 m linear speed result and CODs time in both COD tests. Pearson and Spearman analyses were used to determine the correlations between power-speed-related variables and the CODD. Moreover, independent t-tests and Cohen's d effect size (ES) were used to analyze the differences between the faster and slower players in the CODD. Moderate to strong significant negative correlations were observed between the CODD in the pro-agility test and linear speed at 5 m, 10 m, and 20 m (r = -0.55 to -0.46), while moderate negative significant correlation was found between the zig-zag CODD and SJs (r = -0.37) as well as small positive significant correlation between the CODD in the zig-zag test and the eccentric utilization ratio (EUR) (r = 0.23). Additionally, faster basketball players displayed significantly higher CODD values performing the pro-agility test. In summary, these findings underline the complexity of COD performance and the importance of remodeling traditional training programs in basketball players., (© 2022 Anja Lazić, Slobodan Andrašić, Mima Stanković, Zoran Milanović, Nebojša Trajković, published by Sciendo.)

The aim of this study was to elucidate pattern of attacking actions leading up to goal scoring during the 14 FIFA World Cups from 1966 to 2018. The study analysed 1881 goals scored during a total of 732 matches. We employed observational methodology design. Before goal analysis began, it was developed the observing protocol in which data related to selected variables, by system of notation, was entered after reviewing each individual goal scoring action. The analysis of all video material was carried out independently by four experienced examiners (three of them are Ph.D in sports science and one is Ph.D. candidate in sports science with at least 7 years of coaching and experience as analyst in football). The inter-and intra-observer reliability presented good level of agreement. The kappa values ranged from 0.82 (goal scoring through open play) to 1.00 (action leading up to goal), showing a very high agreement for all performance variables. Interclass correlation was very high (ICC = 0.966, 95% upper and lower confidence intervals were between 0.933 and 1.00). A statistically significant trend ( p  < 0.05) from 1966 to 2018 was identified towards a higher relative frequency of goals scored from set play and collective actions from open play. The Chi-square did not reveal significant differences in the frequency of goal scoring patterns and goal-scoring zones. The results also revealed that the majority of goals were scored between the 76th and 90th minutes of a match (22.7%), from open play (70.5%), inside the penalty area (54.7%), one touch finishing (62.5%), and collective attacks in open play (55.8%). These findings may provide a possible strategic direction for improving goal-scoring performance in football, as well as practical implementation in World Cup tournament preparation., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2023 Mićović, Leontijević, Dopsaj, Janković, Milanović and Garcia Ramos.)

Background: Recreational football is an intense, versatile form of exercise with multiple high- and odd-impact actions. Recreational football is therefore hypothesized to be suitable for bone modeling and bone health., Objective: The aim of the present systematic review and meta-analysis was to evaluate the effects of recreational football on bone mineral density (BMD), bone mineral content (BMC) and bone turnover markers (BTM)., Design: Systematic review and meta-analysis., Data Sources: MEDLINE, PubMed, SPORTDiscus, Web of Science, Cumulative Index to Nursing and Allied Health Literature (CINAHL) and Google Scholar were searched prior to September 2021. A manual database search was also performed using the following key terms, either singly or in combination: recreational football/soccer, street football/soccer, recreational small-sided games, effect, influence, impact, bone turnover markers, bone mineral density, bone turnover marker, bone health, osteogenesis, CTX, osteocalcin, P1NP., Eligibility Criteria for Selecting Studies: Randomised and matched controlled trials with participants allocated to a recreational football group or any other type of training intervention or passive control group were included. The primary outcome measures were total BMD, lower limb BMD, total BMC, lower limb BMC, osteocalcin, procollagen type 1N-terminal propeptide (P1NP) and collagen type 1 cross-linked C-telopeptide (CTX). A total of 17 papers met the inclusion criteria and were included., Statistical Analysis: Comprehensive Meta-analysis V.2 software (Biostat, Englewood, New Jersey, USA) was used for the meta-analyses., Risk of Bias: Agreement between the two reviewers was assessed using RoB2 tool and k statistics for full-text screening and rating of relevance and risk of bias. The k agreement rate between reviewers was k = 0.92., Results: The football interventions included were based on studies having a duration of 12-64 weeks with one 5-year follow-up study and with a training frequency of 1-3 sessions/wk. Training sessions were 45-60 min sessions of 3v3 - 7v7 small-sided games. The subjects covered an age span from 9 to 73 years. Five studies examined recreational football effects in females, nine studies in males and three studies included both sexes. Recreational football training produced a statistically significant effect (mean difference = 0.02 g/cm 2 , 95% confidence interval: 0.00-0.03, P = 0.02) on lower limb BMD and negligible effects for total BMD compared to no-exercise controls. The recreational football effects on total and lower limb BMC, when compared to both no-exercise controls and exercise controls, were negligible. A moderate to large significant increase in osteocalcin, P1NP and CTX was observed for recreational football compared to both no-exercise controls and exercise controls., Conclusion: In conclusion, recreational football training regimes lasting 12-64 weeks have a large osteogenic impact on bone turnover markers in comparison with no-exercise controls as well as exercise controls, and beneficial effects on lower limb BMD compared to no-exercise controls. Short and medium duration recreational football interventions have negligible effects on whole-body BMD and BMC (total and lower limb), with magnitudes similar to those of other exercise modes., (© 2022. The Author(s), under exclusive licence to Springer Nature Switzerland AG.)

Surface modification of magnetic nanoparticles with poly-l-lysine, proline, and tryptophan was used to design potential theranostic agents for the application in cancer diagnosis and radionuclide-hyperthermia therapy. Characterization of bare and functionalized magnetic nanoparticles was performed in detail. The transparency of the examined magnetic nanoparticles was measured in the non-alternating magnetic field for a complete and better understanding of hyperthermia. For the first time amino acid-functionalized magnetic nanoparticles were labeled with theranostic radionuclides 131 I and 177 Lu. The specific absorption rate (SAR) procured for poly-l-lysine functionalized magnetic nanoparticles (SAR values of 99.7 W/g at H 0  = 15.9 kA/m and resonant frequency of 252 kHz) demonstrated their possible application in magnetic hyperthermia. Poly-l-lysine functionalized magnetic nanoparticles labeled with 177 Lu showed the highest radiochemical purity (>99.00 %) and in vitro stability in saline and serum (>98.00 % up to 96 h). The in vivo analysis performed after their intravenous administration in healthy Wistar rats presented good in vivo stability for several days. Encouraging results as well as magnetic and radiochemical properties of 177 Lu-PLL-MNPs (80 °C) justify their further testing toward the potential use as theranostic agents for diagnostic and combined radionuclide-hyperthermia therapeutic applications., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

The purpose of the present study was to examine the influence of selected factors (possession duration, game quarter, defensive pressure, shooting distance from the basket, and heart rate level) on shooting efficiency on Under-19 (U19) and senior level of women's basketball competition (second division). The analysis procedures included five U19 and six senior-level games, containing 224 and 252 shooting attempts, respectively. Binary logistic regression identified the opponent's defensive pressure and shooting distance from the basket as significant predictors of shooting efficiency in both categories. When defensive pressure was high, the chance for the missed shot was 3.5 (95% CI; 1.43-8.52) and 3.19 (95% CI; 1.4-7.26) times more likely than it was under the minimum defensive pressure for U19 and senior category, respectively. Shooting efficiency significantly decreased when the horizontal distance from the basket increased. In U19, a chance for a missed shot was 4.63 (95% CI; 2-10.712) and 5.15 (95% CI; 1.91-13.86) times higher for medium and long-distance (respectively), compared to short-range shooting. In the senior category, the chance for the missed shot was 3.9 (95% CI; 1.83-8.31) and 3.27 (95% CI; 1.43-7.52) times higher for medium and long-distance (respectively) when compared to a short distance. The possession duration, game quarter, and heart rate level were identified as insignificant predictors. Therefore, the aforementioned findings suggest that basketball players and coaches may benefit from designing training sessions where the defender puts pressure on the shooting player as in a real game situation and balanced the frequency of shooting from different distances from the basket., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2022 Vencúrik, Milanović, Lazić, Li, Matulaitis and Rupčić.)

Radiolabelled superparamagnetic iron oxide nanoparticles (SPIONs) are a promising nanomaterial for the development of dual radiation/hyperthermia cancer therapy. To that purpose, flower-shaped SPIONs with an exceptional heating capability were synthesised and coated with citrate, dextran or (3-aminopropyl)triethoxysilane. Both non-coated and coated SPIONs were nontoxic to CT-26 mouse colon cancer cells up to 1.0 mg ml -1 in vitro . In an oscillating magnetic field, citrate-coated SPIONs (CA/SPIONs) displayed the highest heating rate ( SAR  ∼ 253 W g -1 ) and the strongest hyperthermia effects against CT-26 cells. Labelling of the CA/SPIONs by the 90 Y radionuclide, emitting β - radiation with an average/maximum energy of 0.94/2.23 MeV, and deep tissue penetration generated 90 Y-CA/SPIONs intended for the therapy of solid tumours. However, intravenous injection of 90 Y-CA/SPIONs in CT-26 xenograft-bearing mice resulted in low tumour accumulation. On the contrary, intratumoural injection resulted in long-term retention at the injection site. A single intratumoural injection of 0.25 mg CA/SPIONs followed by 30-min courses of magnetic hyperthermia for four consecutive days caused a moderate antitumour effect against CT-26 and 4T1 mouse tumour xenografts. Intratumoural application of 1.85 MBq/0.25 mg 90 Y-CA/SPIONs, alone or combined with hyperthermia, caused a significant ( P  ≤ 0.01) antitumour effect without signs of systemic toxicity. The results confirm the suitability of 90 Y-CA/SPIONs for monotherapy or dual magnetic hyperthermia-radionuclide nanobrachytherapy (NBT) of solid tumours., (© 2022 IOP Publishing Ltd.)