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3,516 results on '"Milan, R"'

6. Factors associated with adherence to swallowing therapy among patients diagnosed with oropharyngeal dysphagia

Author

Uche C. Ezeh, Matina Balou, Tyler Crosby, Paul E. Kwak, and Milan R. Amin

Subjects
health disparities, oropharyngeal dysphagia, swallowing treatment, videofluoroscopic swallowing studies, Otorhinolaryngology, RF1-547, Surgery, RD1-811
Abstract

Abstract Objective The objective of this study is to assess disparities in adherence to swallowing therapy for clinically diagnosed oropharyngeal dysphagia (OD) patients. Methods Analysis was conducted on data from 600 patients with OD and confirmed impairments in swallowing safety and/or efficiency on a videofluoroscopic swallow study. Patients were classified based on their adherence to treatment sessions, defined as the number of swallow treatment sessions attended. The outcome of treatment adherence was categorized into two groups: those who attended fewer than 50% of the prescribed treatment sessions and those who attended 50% or more of the sessions. Continuous variables were presented as mean ± standard deviation or median ± interquartile range. Categorical variables were compared using Pearson chi‐square tests and Fisher's exact test when appropriate. Univariable and multivariable binary logistic regression models were employed to identify factors associated with successful adherence. Results Approximately 79% adhered to swallowing treatment. We found no significant relationship between adherence and age, sex, race, ethnicity, primary language, marital status, insurance status, occupation, median income, distance, education, OD severity, and diagnosis year (p > 0.05). We found no covariables to be significant predictors to swallowing treatment nonadherence in both univariable and multivariable binary regression models (p > 0.05). Conclusion The variables analyzed in this study were not significantly associated with nonadherence to swallow therapy. Nevertheless, our study still addressed an important knowledge gap and future studies would benefit from exploring other relevant socioeconomic and disease‐related factors. Level of evidence Level 4.

Published
2024
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7. Toward 1+1 = 3 with Lean Robotics: The Introduction of a Human-Centered Robotization Method

Author

Wolffgramm, Milan R., Corporaal, Stephan, Groen, Aard J., van Roij, Mitchell J. P. A. M., Rannenberg, Kai, Editor-in-Chief, Soares Barbosa, Luís, Editorial Board Member, Carette, Jacques, Editorial Board Member, Tatnall, Arthur, Editorial Board Member, Neuhold, Erich J., Editorial Board Member, Stiller, Burkhard, Editorial Board Member, Stettner, Lukasz, Editorial Board Member, Pries-Heje, Jan, Editorial Board Member, Kreps, David, Editorial Board Member, Rettberg, Achim, Editorial Board Member, Furnell, Steven, Editorial Board Member, Mercier-Laurent, Eunika, Editorial Board Member, Winckler, Marco, Editorial Board Member, Malaka, Rainer, Editorial Board Member, van Kollenburg, Ton, editor, Kokkinou, Alinda, editor, and McDermott, Olivia, editor

Published
2024
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9. Immune response induced by a Streptococcus suis multi-serotype autogenous vaccine used in sows to protect post-weaned piglets

Author

Alison Jeffery, Mélina Gilbert, Lorelei Corsaut, Annie Gaudreau, Milan R. Obradovic, Simon Cloutier, Marie-Christine Frenette, Charles Surprenant, Sonia Lacouture, Jose Luis Arnal, Marcelo Gottschalk, and Mariela Segura

Subjects
Streptococcus suis, multiserotype autogenous vaccines, sows, bacterin, maternal antibodies, protection, Veterinary medicine, SF600-1100
Abstract

Abstract Streptococcus suis is a bacterial pathogen that causes important economic losses to the swine industry worldwide. Since there are no current commercial vaccines, the use of autogenous vaccines applied to gilts/sows to enhance transfer of passive immunity is an attractive alternative to protect weaned piglets. However, there is no universal standardization in the production of autogenous vaccines and the vaccine formulation may be highly different among licenced manufacturing laboratories. In the present study, an autogenous vaccine that included S. suis serotypes 2, 1/2, 5, 7 and 14 was prepared by a licensed laboratory and administrated to gilts using a three-dose program prior to farrowing. The antibody response in gilts as well as the passive transfer of antibodies to piglets was then evaluated. In divergence with previously published data with an autogenous vaccine produced by a different company, the increased response seen in gilts was sufficient to improve maternal antibody transfer to piglets up to 5 weeks of age. However, piglets would still remain susceptible to S. suis disease which often appears during the second part of the nursery period. Vaccination did not affect the shedding of S. suis (as well as that of the specific S. suis serotypes included in the vaccine) by either gilts or piglets. Although all antibiotic treatments were absent during the trial, the clinical protective effect of the vaccination program with the autogenous vaccine could not be evaluated, since limited S. suis cases were present during the trial, confirming the need for a complete evaluation of the clinical protection that must include laboratory confirmation of the aetiological agent involved in the presence of S. suis-associated clinical signs. Further studies to evaluate the usefulness of gilt/sow vaccination with autogenous vaccines to protect nursery piglets should be done.

Published
2024
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10. Edible Electronic Components Made from Recycled Food Waste

Author

Milan R. Radovanović, Goran M. Stojanović, Mitar Simić, Dragana Suvara, Lazar Milić, Sanja Kojić, and Biljana D. Škrbić

Subjects
biomedical applications, edible electronics, electronic components, food waste sensors, interdigital capacitive sensors, natural materials, Electric apparatus and materials. Electric circuits. Electric networks, TK452-454.4, Physics, QC1-999
Abstract

Abstract A procedure is developed for producing basic electronic components utilizing materials sourced from discarded orange, grapefruit, lemon, apple, banana, potato, and carrot peels. Initially, these materials undergo dehydration, followed by a meticulous pulverization process to obtain fine powder. Natural adhesives like water, honey, sugar, starch, and gelatin are employed to interconnect the materials. Formed substrates are characterized using Scanning Electron Microscopy, Energy Dispersive X‐ray Spectroscopy, and an optical profilometer. Furthermore, the relative permittivity of the materials is determined. Three distinct types of substrates, derived from the aforementioned peels, are crafted in varying dimensions. Substrates measuring 4 cm × 2.5 cm host interdigital capacitive sensors, whereas larger 6 cm × 3.5 cm substrates accommodate inductor‐capacitor (LC) sensors. Each of the six samples undergoes individual dry testing, while LC sensors are additionally tested with the post‐application of artificial saliva and mouthwash liquids. The sensor's characterization involves measurement of impedance and phase angle for all samples. Capacitance is additionally measured for capacitors, and inductance for LC circuits. These assessments are carried out within the frequency range spanning from 1 MHz to 400 MHz. The objective is the development of fully functional electronic components, derived from discarded edible items, fostering sustainable practices, and finding applications in biomedicine.

Published
2024
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11. The significance of determining the Intraoperative level of parathormone during surgical treatment of hyperparathyroidism

Author

Janičić Stefan D., Stepanović Kristina M., Manojlović Mia S., Bjelajac Dean P., Pejaković Slađana M., Mirković Milan R., Tomić-Naglić Dragana D., Bajkin Ivana A., and Ičin Tijana S.

Subjects
primary hyperparathyroidism, intraoperative parathyroid hormone monitoring, parathyroidectomy, Therapeutics. Pharmacology, RM1-950
Abstract

Introduction: Surgical treatment is the method of choice for treating primary hyperparathyroidism. Determining parathyroid hormone (PTH) levels intraoperatively allows for real-time monitoring of the functional activity of parathyroid gland tissue and facilitates minimally invasive parathyroidectomy. Aim: Our study aimed to investigate the significance of intraoperative parathyroid hormone level determination in achieving physiological parathyroid hormone levels and cure. Material and Methods: A retrospective academic study analyzed 70 patients from the Clinic for Endocrinology, Diabetes, and Metabolic Disorders at the University Clinical Center of Vojvodina who underwent parathyroidectomy. Participants were divided into control and study groups based on whether intraoperative parathyroid hormone levels were determined. Demographic and laboratory-clinical data from medical records were statistically analyzed. Results: In the study group, the median of parathyroid hormone level was 104.30 pg/mL, while in the control group, it was 128.09 pg/mL; no statistically significant difference was observed (p=0.380). There was no statistically significant difference between groups regarding the number of reoperations indicated and the diagnosis of persistent hyperparathyroidism (p=0.355). A statistically significant difference in treatment outcomes was found among participants who met the Miami and Dual protocol criteria. Conclusion: Determining parathyroid hormone levels during surgical treatment while meeting the criteria of current protocols can contribute to a higher cure rate. A more extensive prospective study is required for a more in-depth analysis of our healthcare center's experience.

Published
2024
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12. In silico studies of phycobilins as potential candidates for inhibitors of viral proteins associated with COVID-19

Author

Jovanović Vesna B., Nikolić Milan R., and Stojanović Srđan Đ.

Subjects
phycobilins, covid-19, inhibitors, proteins, in silico studies, Chemistry, QD1-999
Abstract

In this in silico study, it was investigated whether phycobilins (phycocyanobilin, phycoerythrobilin and phycourobilin) could be inhibitors of the activity of the main proteins of the SARS-CoV-2 virus. All chromophores exhibited a binding energy value of ≥–37 kJ mol-1 for PLpro-WT, PLpro- -C111S, helicase-ANP binding site, Nsp3-macrodomain, Nsp3-MES site and Nsp10/14-N7-Mtase. Phycocyanobilin showed the highest binding energy of –44.77 kJ mol-1 against the target protein PLpro-C111S. It was found that, apart from the hydrogen bonds and hydrophobic interactions, phycobilins also form electrostatic interactions with the SARS-CoV-2 proteins. The network of non-covalent interactions was found to be important for the stability of the examined virus proteins. All phycobilins have good pharmacokinetic and drug- -likeness properties. This study’s results suggest that the screened phycobilins could serve as promising drugs for the treatment of COVID-19 with further rigorous validation studies.

Published
2024
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16. Factors Associated With Coronary Angiography Performed Within 6 Months of Randomization to the Conservative Strategy in the ISCHEMIA Trial

Author

Pracoń, Radosław, Spertus, John A., Broderick, Samuel, Bangalore, Sripal, Rockhold, Frank W., Ruzyllo, Witold, Demchenko, Elena, Nageh, Thuraia, Grossman, Gabriel Blacher, Mavromatis, Kreton, Manjunath, Cholenahally N., Smanio, Paola E.P., Stone, Gregg W., Mancini, G.B. John, Boden, William E., Newman, Jonathan D., Reynolds, Harmony R., Hochman, Judith S., Maron, David J., Doan, John, Linefsky, Jason, Lee, Raven, Patel, Risha, Miller, Todd, Yang Cho, So, Milbrandt, Susan, Shelstad, Dawn, Banerjee, Subhash, Kamath, Preeti, Tejani, Ishita, Cobos, Stanley E., Quiles, Kirsten J., Dwyer, Raven R., Donnino, Robert M., Espinosa, Dalisa, Phillips, Lawrence M., Saric, Muhamed, Abdul-Nour, Khaled, Schley, Allison, Golden, Heather, Stone, Peter H., Osseni, Hermine, Wiyarand, Charlene, Douglass, Peter, Pomeroy, Hayley, Craft, Alexandra, Harvey, Bethany, Jang, James J., Anaya, Olivia, Yee, Gennie, Goold, Phoebe, Weitz, Steven, Giovannone, Steven, Pritchard, Lori, Arnold, Suzanne, Gans, Rosann, Henry O’Keefe, Jr, James, Kennedy, Paul, Shapiro, Michael D., Ganesan, Shobana, Schlichting, David, Naher, Aynun, El-Hajjar, Mohammad, Sidhu, Mandeep S., Fein, Steven A., Stewart, Wendy L., Torosoff, Mikhail T., Salmi, Kristin M., Lyubarova, Radmila, Mookherjee, Sulagna, Drzymalski, Krzysztof, McFalls, Edward O., Garcia, Santiago A., Bertog, Stefan C., Johnson, Debra K., Siddiqui, Rizwan A., Herrmann, Rebekah R., Ishani, Areef, Hansen, Ronnell A., Georges Khouri, Michel, Arges, Kristine, LeFevre, Melissa, Tomfohr, Jennifer, Goldberg, Jonathan L., Ann Byrne, Kimberly, Zappernick, Taissa, Goldweit, Richard, Canada, Sallie, Kakade, Meghana, Mieses, Patricia, Cobos, Stanley E., Dwyer, Raven R., Cohen, Ronny A., Espinosa, Dalisa, Mirrer, Brooks, Quiles, Kirsten J., Navarro, Victor, Rantinella, Magdalena, Rodriguez, Jessica, Mancilla, Olivia, Winchester, David E., Stinson, Susan, Kronenberg, Marvin, Weyand, Terry, Rogal, Philip, Crook, Sherron C., McFarren, Christopher, Heitner, John F., Ho, Jean, Khan, Saadat, Mohamed, Mahmoud, Dauber, Ira M., Soltau, Mary R., Rose, Delsa K., Wimmer, Rebecca J., Siegel, Kathy E., Derbyshire, Susan, Cannan, Charles, Dixon, Michelle, Leonard, Gerald, Sudarshan, Sriram, Heard, Ciarra, Gabriel, Viviana, Desire, Sukie, Mehta, Puja K., McDaniel, Michael, Rashid, Fauzia, Lerakis, Stamatios, Asier, Senait, Quyyumi, Arshed, Patel, Keyur, Wenger, Nanette K., Hedgepeth, Chester M., Gillis, Jennifer, Hurlburt, Heather, Manocchia, Megan, Rosen, Alan, Moore, Susan, Congdon, Elizabeth, Sahul, Zakir, Brandt, Gail, Marchelletta, Nora, Wippler, Kristina, Booth, David, Taul, Yvonne, Leung, Steve, Isaacs, Jennifer, Abdel-Latif, Ahmed, Bulkley, Viktoria, Reda, Hassan, Rodgers, Caroline, Ziada, Khaled, Setty, Sampoornima, Halverson, Kimberly E., Roraff, Christine, Thorsen, Jonean, Barua, Rajat S., Ojajuni, Amarachi, Olurinde, Oni, Surineni, Kamalakar, Hage, Fadi, Valaiyapathi, Badhma, Caldeira, Christiano, Davies, James E., Leesar, Massoud, Heo, Jaekyeong, Iskandrian, Amy, Al Solaiman, Firas, Singh, Satinder, Dajani, Khaled, Kartje, Carol M., El-Hajjar, Mohammad, Mesropian, Paul Der, Sacco, Joseph, Rawlins, Michele, McCandless, Brian, Thomson, Jennifer, Orgera, Marisa, Sidhu, Mandeep S., Colleen Rogge, Mary, Arif, Imran, Bunke, Julie, Kerr, Hanan, Unterbrink, Kendra, Fannon, Jacqueline, Burman, Cynthia, Trejo, Jorge F., Dubin, Marcia F., Fletcher, Gerald, Lane, Gary E., Neeson, Lynn M., Parikh, Pragnesh P., Pollak, Peter M., Shapiro, Brian P., Landolfo, Kevin, Gemignani, Anthony, Beaudry, Sarah, O’Rourke, Daniel, Meadows, Judith L., Tirado, Stephanie A., Halliday, Janet, Julian, Pamela, Call, Jason T., Lane, Stephanie M., Stanford, Jennifer L., Hannan, Joseph, Bojar, Robert, Arsenault, Patricia, Kumar, Deepti, Sigel, Pamela, Mukai, John, Martin, Edward T., Brooks, Miriam, Vorobiof, Gabriel, Douangvila, Ladda, Gevorgyan, Rubine, Moorman, Alec, Ranjbaran, Fatima, Smith, Bryn, Ohmart, Carly, Kinlay, Scott, Hamburger, Robert J., Rocco, Thomas P., Ly, Samantha, Bhatt, Deepak L., Quinn, Margot C., Croce, Kevin, Temiyasathit, Sara, Quin, Jacquelyn A, Do, Jacquelyn, Anumpa, Jati, Tobin, Desiree, Zenati, Marco, Faxon, David P, Rayos, Glenn, Langdon, Jennifer, Werner Bayer, Marcia, Seedhom, Ashraf, O’Malley, Amanda, Sullenberger, Lance, Orvis, Erin, Kumkumian, Gregory, Murphy, Mandy, Greenberg, Ann, Iraola, Margaret, Sedlis, Steven P., Maranan, Leandro C., Donnino, Robert M., Lorin, Jeffrey, Tamis-Holland, Jacqueline E., Malinay, Ammy, Kornberg, Robert, Leber, Robert, Saba, Souheil, Edillo, Candice P., Lee, Michael W., Small, Delano R., Nona, Wassim, Alexander, Patrick B., Rehman, Iram, Badami, Umesh, Ostrander, Ann, Wasmiller, Stephanie, Marzo, Kevin, Drewes, Wendy, Patel, Dipti, Robbins, Inga H., Levite, Howard A., White, Jackie M, Shetty, Sanjay, Hallam, Alison, Patel, Mayuri, Hamroff, Glenn S., Spooner, Benjamin J, Hollenweger, Linda M, Little, Raymond W., Little, Holly, Zimbelman, Brandi D., Little, Tiffany, Lui, Charles Y., Eskelson, Nona A, Smith, Brigham R., Vezina, Daniel P., Khor, Lillian L., Abraham, Josephine D., Bull, David A., McKellar, Stephen H., Booth, David, Taul, Yvonne, Kotter, John, Rodgers, Caroline, Abdel-Latif, Ahmed, Isaacs, Jennifer, Bulkley, Viktoria, Hu, Bob, Kaneshiro, Renee, Labovitz, Arthur J., Berlowitz, Michael, Kirby, Bonnie J., Rogal, Philip, Tran, Nhi N., McFarren, Christopher, Jahrsdorfer, Catherine, Matar, Fadi, Caldeira, Christiano, Rodriguez, Fatima, Yunis, Reem, Schnittger, Ingela, Patro, Jhina, Fearon, William F., Deedwania, Prakash, Vega, Antonia, Reddy, Kiran, Sweeny, Joseph, Bloise-Adames, Hugo, Jimenez, Santa, Saint Vrestil, Nicole, Bhandari, Reyna, Spizzieri, Christopher, Schade, Danielle, Yost, Roxanne, Hochberg, Claudia P, Beardsley, Paula, Fine, Denise, Salerno, William D., Tancredi, Jana, Arakelian, Patricia, Mathus, Susan, O’Neill, Deborah, Wyman, Ray, Burkhardt, Joy, Hosino, Suellen, Lubyanaya, Oksana A., Salas, Jose D., Zarka, Amer, Aguirre, Maria, Shah, Anil V., Dhawan, Manu, Parra, Diana, Tran, Tri, Haldis, Thomas, Weick, Catherine, Fowler-Lehman, Katie, Spitzer, Natalie, Riedberger, Casey, Weick, Catherine, Kohn, Jeffrey A., Cobos, Stanley E., Dwyer, Raven R., Espinosa, Dalisa, Quiles, Kirsten J., Girotra, Saket, Drum, Carrie, Miller-Cox, Kimberly, Ollinger, Amy, Almousalli, Omar, Capasso-Gulve, Elizabeth, Melanie Loehr, Alaine, Mosley, Marlowe, Krishnam, Mayil S., Heydari, Shirin, Milliken, Jeffrey C., Lundeen, Andrea M., Patel, Pranav M., Karanjah, Edgar, Seto, Arnold H., Marfori, Wanda C., Harley, Kevin T., Hernandez-Rangel, Eduardo, Gibson, Michael A., Singh, Pam, Allen, Byron J., Coram, Rita, Marie Webb, Anne, Fridell, Ellie, Wilson, Heidi, Thomas, Sabu, Kim, Angela, Schwartz, Ronald G, Wilmot, Patrick, Chen, Wei, El Shahawy, Mahfouz, Stevens, Ramona, Stafford, James, Black, Loriane, Abernethy, William B., Hull, Amber B., Lim, Olivia J., Tucker, Helen C., Putnam, Natasha C., Hall, Linda L., Cauthren, Tia, Tucker, Trish, Zurick, Andrew, Horton, Hollie, Orga, Jan, Meyer, Thomas M., White, Joyce R., Morford, Ronald G., Baumann, Cynthia, Rutkin, Bruce, Seeratan, Vidya, Bokhari, Sabahat, Jimenez, Magnolia, Sokol, Seth I., Schultz, Cidney, Meisner, Jay, Russo, Jeanne, Hamzeh, Ihab, Misra, Arunima, Huda, Zohra, Wall, Matthew, Boan, Araceli, Lenges De Rosen, Veronica, Alam, Mahboob, Turner, Michael C., Hinton, Christine R, Mulhearn, Thomas J., Good, Arnold P., Archer, Beth A., Dionne, Julia S., Allardyce, Cheryl A., Sikora, Lindsey N., Czerniak, Jennifer H., Mull, Jennifer A., Ferguson, Elizabeth, Laube, Frances, Shammas, Nicolas W., Shammas, Gail A, Christensen, Lori, Park, Holly, Chilton, Robert, Hecht, Joan, Nguyen, Patricia K., Vo, Davis, Hirsch, James, Jezior, Matthew, Bindeman, Jody, Salkind, Sara, Espinosa, Dalisa, Desimone, Lori-Ann, Gordon, Paul C., Felix-Stern, Lina, Crain, Thomas, Gomes, Jassira, Gordon, Catherine, Stenberg, Robert, Mann, Aimee, McCreary, Theresa, Pedalino, Ronald P., Cobos, Stanley E., Dwyer, Raven R., Espinosa, Dalisa, Quiles, Kirsten J., Wiesel, Joseph, Cobos, Stanley E., Dwyer, Raven R., Espinosa, Dalisa, Quiles, Kirsten J., Juang, George J., Gopaul, Candace, Hultberg, Karen, Huk, Tauqir, Hussain, Afshan, Al-Amoodi, Mohammed, Zambrano, Yesenia, Medina Rodriguez, Sarah, Milner, Trudie, Wohns, David, Mulder, Abbey, Van Oosterhout, Stacie, Lader, Ellis W., Meyer, Martha, Mumma, Michael, Clapp, Nancy L., Barrentine, Heather, Dharmarajan, Lekshmi, Jose, Jenne M., Cobos, Stanley E., Dwyer, Raven R., Espinosa, Dalisa, Quiles, Kirsten J., Manchery, Jenne, McGarvey Jr, Joseph F.X., McKinney, Vera, Schwarz, Linda, Downes, Thomas R., Kaczkowski, Scott M., Luckasen, Gary J., Jaskowiak, Adam J., Klitch, Joel, Cheong, Benjamin, Dees, Debra, Potluri, Srinivasa, Vasquez, Precilia, Mastouri, Ronald A., Breall, Jeffery A., Hannemann, Elise L., Revtyak, George E., Mae Foltz, Judy, Bazeley, Jonathan W., Li, Dayuan, DeRosa, Emily, Jorgenson, Beth, Riestenberg-Smith, Joyce, Giedd, Kenneth, Old, Wayne, Bariciano, Rebecca, Burt, Francis, Sokhon, Kozhaya, Waldron, Jessica, Mayon, Michelle, Gopal, Deepika, Valeti, Uma S., Ann Peichel, Gretchen, Kobashigawa, Jon, Starks, Brandy, Garcia, Lucilla, Thottam, Maria, Bhargava, Balram, Anand, Anjali, Chakanalil Govindan, Sajeev, Raj, Janitha, Gopalan Nair, Rajesh, Ravindran, Reshma, Rajalekshmi, VS, Nataraj, Nandita, Moorthy, Nagaraja, Nayak, Soundarya, Mylarappa, Mahevamma, Narayanappa, Suryaprakash, Pandit, Neeraj, Bajaj, Sheromani, Kumar Nath, Ranjit, Yadav, Vandana, Mishra, Girish, Dwivedi, S.K., Tewari, Roma, Narain, V.S., Mishra, Meenakshi, Chandra, Sharad, Patel, Shivali, Singh, Suman, Wander, Gurpreet S., Tandon, Rohit, Ralhan, Sarju, Kaur, Baljeet, Aslam, Naved, Gupta, Sonika, Goyal, Abhishek, Bhargava, Balram, Suvarna, Chandini, Karthikeyan, G., Ramakrishnan, S., Seth, Sandeep, Yadav, Rakesh, Singh, Sandeep, Roy, Ambuj, Parakh, Neeraj, Kumar Verma, Sunil, Narang, Rajiv, Mishra, Sundeep, Naik, Nitish, Sharma, Gautam, Kumar Choudhary, Shiv, Patel, Chetan, Gulati, Gurpreet, Sharma, Sanjeev, Bahl, V K, Mathew, Anoop, Mannekkattukudy Kurian, Binoy, Punnoose, Eapen, Avdhoot Gadkari, Milind, Rupesh Karwa, Sheetal, Gadage, Siddharth, Kolhe, Suvarna, Umesh Pillay, Tapan, Satheesh, Santhosh, Vindhya, R. J., Jain, Peeyush, Seth, Ashok, Singh Meharwal, Zile, Mathur, Atul, Verma, Atul, Kaul, Upendra, Bhatia, Mona, Sachdeva, Ankush, Indira Devi, Thounaojam, Jungla, Nungshi, Christopher, Johann, Manjula Rani, K., Menon, Rajeev, Sowjanya Reddy, M., Kumar, Nirmal, Preethi, K., Oomman, Abraham, sidh, Rinu R, Mao, Robert, Ramakrishnan, T., Solomon, Hilda, Francis, Rajesh, Naik, Sudhir, Vamshi, Priya P., Parveen Khan, Sajeeda, Christopher, Johann, Preethi, Kotiboinna, Kumar, Nirmal, Grant, Purvez, Hande, Shweta, Sonawane, Poonam, Kachru, Ranjan, Dubey, Abhishek, Rawat, Kavita, Kumar, Ajit, Ganapathi, Sanjay, K, Jayakumar, CP, Vineeth, Sivadasanpillai, Harikrishnan, Chacko, Manas, Sasidharan, Bijulal, Babu, Suresh, TR, Kapilamoorthy, Christopher, Johann, Reddy, Sowjanya, Polamuri, Praneeth, Rani, Manjula, Kaul, Upendra, Arambam, Priyadarshani, Singh, Bebek, Senior, Roxy, Fox, Keith AA, Young, Grace M., Carruthers, Kathryn, Senior, Roxy, Elghamaz, Ahmed, Gurunathan, Sothinathan, Karogiannis, Nikolaos, Young, Grace M., Shah, Benoy N, Kinsey, Christopher, Trimlett, Richard HJ, Kavalakkat, Raisa, Rubens, Michael B, Evans, Jo, Nicol, Edward D, Hassan, Ikraam, Mittal, Tarun K, Hampson, Reinette, Andreas Gamma, Reto, Williams, Sarah, Holland, Kim, Swan, Karen, de Belder, Mark A, Atkinson, Bev, Thambyrajah, Jeet, Kunhunny, Swapna, Davies, John R, Lindsay, Steven J., Atkinson, Craig, Kurian, John, Krannila, Carita, Jamil, Haqeel, Vinod, Manitha, Raheem, Osama, Hoye, Angela, Chaytor, Lisa, Cox, Leanne, Morrow, Julie, Rowe, Kay, Donnelly, Patrick, Kelly, Stephanie, Valecka, Bernardas, Regan, Susan, Turnbull, Dawn, Chauhan, Anoop, Fleming, Catherine, Ghosh, Arijit, Gratrix, Karen, Preston, Stephen, Barr, Craig, Cartwright, Anne, Alfakih, Khaled, Knighton, Abigail, Byrne, Jonathan, Martin, Katherine, Webb, Ian, Henriksen, Peter, Flint, Laura, Harrison, James, OKane, Peter, Lakeman, Nicki, Ljubez, Anja, de Silva, Ramesh, Conway, Dwayne S. G., Wright, Judith, Exley, Donna, Sirker, Alexander A, Andiapen, Mervyn, Richards, Amy J., Hoole, Stephen P, Wong, Lisa, Witherow, Fraser N., Munro, Melanie J., Johnston, Nicola, Harbinson, Mark, McEvoy, Michelle, Walsh, Simon, Brown, Caroline, Douglas, Hanna, Luckie, Matthew, Charles, Thabitha, Kolakaluri, Laurel, Phillips, Hannah, Sobolewska, Jolanta, Morby, Louise, Hallett, Karen, Corbett, Carolyn, Winstanley, Lynne, Jeetley, Paramjit, Smit, Angelique, Patel, Niket, Kotecha, Tushar, Travill, Christopher, Gent, Susan, Karimullah, Iqbal, Hussain, Nafisa, Al-Bustami, Mahmud, Braganza, Denise, Haines, Fiona, Taaffe, Joanne, Henderson, Robert, Burton, Jane, Pointon, Kate, Colton, Maria, Naik, Surendra, King, Rachel, Mathew, Thomas, Brown, Ammani, Docherty, Andrew, Berry, Colin, McCloy, Lisa, Collison, Damien, Robb, Kate, Roditi, Giles, Paterson, Craig, Crawford, Wenda, Kelly, Joanne, McGregor, Lorraine, Moriarty, Andrew J, Mackin, Anne, Glover, Jason D., Knight, Janet P, Pradhan, Jiwan, Mikhail, Ghada, Bose, Tuhina, Francis, Darrel P., Dzavik, Vladimir, Goodman, Shaun, Gosselin, Gilbert, Gosselin, Gilbert, Proietti, Anna, Brousseau, Myriam, Corfias, Magalie, Blaise, Patricia, Harvey, Luc, Diaz, Ariel, Rheault, Philippe, Barrero, Miguel, Gagné, Carl-Éric, Alarie, Patricia, Pépin-Dubois, Yanek, Arcand, Linda, Costa, Ricardo, Roy, Isabelle, Tung Sia, Ying, Montpetit, Estelle, Lemay, Catherine, Gisbert, Alejandro, Gervais, Pierre, Rheault, Alain, Drouin, Katia, Carl Phaneuf, Denis, Bergeron, Christine, Gosselin, Gilbert, Shelley, Christine, Masson, Christine, Garg, Pallav, Carr, Sandy, Bone, Catherine, Chow, Benjamin J.W., Moga, Ermina, Hessian, Renee C., Kourzenkova, Janetta, Beanlands, Rob S., Walter, Olga, Davies, Richard F., Bainey, Kevin R., Hogg, Norma, Welsh, Suzanne, Cheema, Asim N., Bagai, Akshay, Wald, Ron, Goodman, Shaun, Kushniriuk, Khrystyna, Joseph Graham, John, Hussain, Mohammed, Peterson, Mark, Bello, Olugbenga, Chow, Chi-Ming, Abramson, Beth, Nazir Cheema, Asim, Syed, Ishba, Hussain, Mohammed, Kushniriuk, Khrystyna, Cha, James, Otis, Judy, Otis, Rebecca, Howarth, Andrew G, Seib, Michelle M, Rivest, Sandra M, Sandonato, Rosa, Wong, Graham, Chow, Jackie, Starovoytov, Andrew, Uchida, Naomi, Meadows, Ngaire, Uxa, Amar, Asif, Nadia, Tavares, Suzana, Galiwango, Paul, Bozek, Bev, Kassam, Saleem, Shier, Maria, Mukherjee, Ashok, Larmand, Lori-Ann, Ricci, A. 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A., Oliveira, Natalia S, Lima, Rodolfo G. S. 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Marin, Valdés Chávarri, M., Quintana Giner, M., Tello Montolliu, A., Romero Aniorte, A.I., Pinar Bermudez, E., Rivera Caravaca, JM., De La Morena, G., Gracida Blancas, Montserrat, Cañavate, Olga, Guerrero, Sonia, Riera, Silvia, Enrique Castillo Luena, Jose, Enrique Castillo Luena, Jose, Lasala, Maria, Fernandez-Aviles, Francisco, Lorenzo, Maria, Sobrino, Olga, Vazquez, Alexandra, Jiang, Lixin, Chen, Jiyan, Dong, Haojian, He, Peiyu, Xia, Chunli, Yang, Junqing, Zhong, Qi, Wu, Yongjian, Tian, Yanmeng, Li, Dongze, Ma, Yitong, Li, Xiaomei, Yang, Yining, Ma, Xiang, Yu, Zixiang, Zhao, Qian, Ji, Zheng, Li, Chunguang, Zhang, Lei, Zhao, Yu, Zhu, Bolin, Yang, Xinchun, Chen, Mulei, Chi, Hongjie, Wang, Yang, Zhang, Jing, Lin, Wenhua, Jing, Rui, Liu, Jingjing, Zeng, Hesong, Zhou, Qiang, Xu, Chang, Li, Zhuxi, Li, Junhua, Xiong, Luyang, Fu, Xin, Gao, Dan, Jiang, Dengke, Leng, Ran, Wang, Xutong, Yuan, Qianqian, Zhang, Lili, Yang, Bin, Bai, Ziliang, Li, Jianhua, Qi, Jie, Wang, Fei, Wang, Haitao, Yang, Bin, Yue, Zhou, Zhang, Zhulin, Wang, Songtao, Dong, Yumei, Mao, Jiajia, Zhang, Bin, Cheng, Gong, Li, Xiuhong, Yao, Xiaowei, Zhong, Nier, Zhou, Ning, Zhao, Yulan, Huang, Yaping, Zhou, Panpan, Fang, Xuehua, Su, Wei, Zeng, Qiutang, Kunwu, Yu, Peng, Yudong, Su, Xin, Su, Xi, Wang, Chen, Zhao, Yunhai, Li, Qingxian, Geng, Yaming, Wang, Yanfu, Nie, Shao-ping, Fan, Jing-yao, Feng, Si-ting, Wang, Xiao, Yan, Yan, Zhang, Hui-min, Yu, Qin, Chi, Lingping, Liu, Fang, Wang, Jian’an, Chen, Han, Jiang, Jun, Li, Huajun, Wang, Jian’an, Han, Yechen, Xu, Lihong, Zhang, Shuyang, Liu, Zhenyu, Liu, Zhenyu, Chen, Gang, Hu, Rongrong, Maggioni, Aldo P., Piero Perna, Gian, Pietrucci, Francesca, Marini, Marco, Gabrielli, Gabriele, Provasoli, Stefano, Di Donato, Anna, Verna, Edoardo, Monti, Lorenzo, Nardi, Barbara, Di Chiara, Antonio, Pezzetta, Francesca, Mortara, Andrea, Casali, Valentina, Galvani, Marcello, Attanasio, Chiara, Ottani, Filippo, Sicuro, Marco, Leone, Gianpiero, Pisano, Francesco, Bare, Cristina, Calabro, Paolo, Fimiani, Fabio, Formisano, Tiziana, Tarantini, Giuseppe, Barioli, Alberto, Cucchini, Umberto, Ramani, Federica, Luigi Andres, Anto, Racca, Emanuela, Rolfo, Fabrizio, Goletto, Cecilia, Briguori, Carlo, De Micco, Francesca, Amati, Roberto, Di Marco, Stefano, Vergoni, William, Tricoli, Martina, Russo, Aldo, Villella, Massimo, Fanelli, Raffaele, Douglas White, Harvey, Alsweiler, Caroline, Poh, Kian-Keong, Chai, Ping, Lau, Titus, Loh, Joshua P., Tay, Edgar L., Teoh, Kristine, Tan, Sik-Yin V, Teo, Lynette L., Sia, Winnie C, Ong, Ching-Ching, Leong, Audrey W, Wong, Raymond C., Loh, Poay-Huan, Kofidis, Theodoros, Xian Chan, Wan, Hui Chan, Koo, Foo, David, Hai Yan, Li, Loh Kwok Kong, Jason, Min Er, Ching, Haider Jafary, Fahim, Chua, Terrance, Ismail, Nasrul, Tun Kyaw, Min, Yip, Deborah, Doerr, Rolf, Doerr, Rolf, Stumpf, Juergen, Grahl, Dorit, Matschke, Klaus, Guenther, Franziska, Simonis, Gregor, Bonin, Kerstin, Kadalie, Clemens T., Sechtem, Udo, Wenzelburger, Ina, Ong, Peter, Gruensfelder, Susanne, Christian Schulze, P., Goebel, Bjoern, Lenk, Karsten, Nickenig, Georg, Sinning, Jan-Malte, Weber, Marcel, Werner, Nikos, Marthe Lang, Irene, Huber, Kurt, Schuchlenz, Herwig, Steinmaurer, Gudrun, Weikl, Stefan, Marthe Lang, Irene, Winter, Max-Paul, Andric, Tijana, Huber, Kurt, Tscharre, Maximilian, Jakl-Kotauschek, Gabriele, Wegmayr, Claudia, Jäger, Bernhard, Egger, Florian, Keltai, Matyas, Vertes, Andras, Sebo, Judit, Davidovits, Zoltan, Matics, Laszlone, Varga, Albert, Ágoston, Gergely, Fontos, Geza, Dekany, Gabor, Merkely, Bela, Bartykowszki, Andrea, Maurovich-Horvat, Pal, Kerecsen, Gabor, Jakal, Agnes, Hinic, Sasa, Djokic, Jelena, Zdravkovic, Marija, Mudrenovic, Vladan, Crnokrak, Bogdan, Beleslin, Branko D., Boskovic, Nikola N., Djordjevic-Dikic, Ana D., Petrovic, Marija T., Giga, Vojislav L., Dobric, Milan R., Stepanovic, Jelena J., Markovic, Zeljko Z., Mladenovic, Ana S., Cemerlic-Adjic, Nada, Velicki, Lazar, Kamenica, Sremska, Pupic, Ljiljana, Davidović, Goran, Simović, Stefan M., Vučić, Rada, Dekleva, Milica Nikola, Martinovic, Miroslav Stevo, Stevanovic, Gordana, Stankovic, Goran, Dobric, Milan, Apostolovic, Svetlana, Martinovic, Sonja Salinger, Stanojevic, Dragana, Escobedo, Jorge, Jesús-Pérez, Ramon de, Juarez, Benito, Baleón-Espinosa, Rubén, Campos-Santaolalla, Arturo S, Durán-Cortés, Elihú, Flores-Palacios, José M, García-Rincón, Andrés, Jiménez-Santos, Moisés, Peñafiel, Joaquín V, Ortega-Ramírez, José A, Valdespino-Estrada, Aquiles, Rosas, Erick Alexánderson, Canales Brassetti, María Fernanda, Vences Anaya, Diego Adrián, García, María Pérez, Carvajal Juarez, Isabel Estela, Rovalo, Magdalena Madero, Morales Rodríguez, Erick Donato, Selvanayagam, Joseph B., Rankin, Jamie, Murphy, Deirdre, Selvanayagam, Joseph B., Lee, Sau, Joseph, Majo X., Thomas, Prince, Thambar, Suku T., Chaplin, Melissa D, Boer, Stephanie C, Beltrame, John F., Stansborough, Jeanette K., Black, Marilyn, Hillis, Graham S., Bonner, Michelle M., Ireland, Kim F., Venn-Edmonds, Clare, Steg, Philippe-Gabriel, Abergel, Helene, Juliard, Jean-Michel, Thobois, Corine, Pasteur, C.H. Louis, Thuaire, Christophe, Tachot, Emilie, Dutoiu, Téodora, Laure, Christophe, Vassaliere, Christel, Steg, Philippe Gabriel, Abergel, Helene, Juliard, Jean-Michel, Fuentes, Axelle, Slama, Michel S., Eliahou, Ludivine, Cedex, Clamart, El Mahmoud, Rami, Dubourg, Olivier, Michaud, Pierre, Nicollet, Eric, Hadjih, Sarah, Cedex, Corbeil-Essonnes, Goube, Pascal, Brito, Patricia, Barone-Rochette, Gilles, Barone-Rochette, Gilles, Furber, Alain, Cornet, Charles, Bière, Loïc, Rautureau, Jeremy, Juceviciene, Agne, Kalibataite-Rutkauskiene, Irma, Keinaite, Laura, Laucevicius, Aleksandras, Laukyte, Monika, Celutkiene, Jelena, Mikolaitiene, Gelmina, Smigelskaite, Akvile, Tamasauskiene, Ilona, Urboniene, Agne, Kedhi, Elvin, Klinieken, Isala, Timmer, Jorik, Bouwhuis, Ilse, Hermanides, Rik, Nijmeijer, Lia, Kaplan, Eliza, Riezebos, Robert K., Samadi, Pouneh, Schoep Jeannette, J. M., Dongen, Elise van, Janzen, Elisabeth M., Niehe, Sander R., Suryapranata, Harry, Ahoud, Sandra, Vugt, Stijn van, Ramos, Ruben, Santa Marta, Hospital de, Cacela, Duarte, Santana, Ana, Fiarresga, Antonio, Sousa, Lidia, Marques, Hugo, Patricio, Lino, Selas, Mafalda, Bernanrdes, Luis, Silva, Filipa, Rio, Pedro, Freixo, Cláudia, Carvalho, Ramiro, Ferreira, Rui, Silva, Tiago, Rodrigues, Ines, Modas, Pedro, Portugal, Guilherme, Fragata, Jose, Pinto, Fausto J., Cabrita, Inês Zimbarra, Menezes, Miguel Nobre, Rocha, Andreia, Lopes, Guilhermina Cantinho, Figueiras, Francisca Patuleia, Almeida, Ana Gomes, Coelho, Andreia, CanVas Silva, Pedro, Capinha, Marta, Nobre, Angelo, Caetano, Maria Inês, Francisco, Ana Rita, Silva, Susana, Ferreira, Nuno, de Gaia, Vila Nova, Lopes, Ricardo L., Diaz, Rafael, Guzman, Luis, Tinnirello, Veronica, Figal, Julio César, Nicolás Mungo, Matías, Buenos Aires, Ciudad Autonoma de, Méndiz, Oscar, Cortés, Claudia, Favaloro, Roberto René, Alvarez, Carlos, Garcia, Marina, Blanca, Bahia, Courtis, Javier, Godoy, Valeria, Zeballos, Gabriela, Schiavi, Lilia, Actis, Maria Victoria, Rubio, Mariano, Scaro, Graciela, White, Harvey Douglas, Alsweiler, Caroline, Devlin, Gerard Patrick, Low, Liz, Fisher, Raewyn, Scales, Jayne, Abercrombie, Kirsty, Stewart, Ralph Alan Huston, Howell, Leah, White, Harvey Douglas, Patten, Cathrine, Benatar, Jocelyne, Kedev, Sasko, Mitevska, Irena Peovska, Kostovska, Elizabeta Srbinovska, Pejkov, Hristo, Held, Claes, Held, Claes, Eggers, Kai, Frostfelt, Gunnar, Björklund, Christina, Johnston, Nina, Andreasson, Maria, Olsowka, Maciej, Essermark, Marie, Åkerblom, Axel, Soveri, Inga, Aspberg, Johannes, Persson, Liselotte, Beyar, Rafael, Sharir, Tali, Nikolsky, Eugenia, Sharir, Tali, Harel, Or, Elian, Dan, Kerner, Arthur, Bentzvi, Margalit, Massalha, Samia, Helmer, Ludmila, Kohsaka, Shun, Fukuda, Keiichi, Ueda, Ikuko, Kohsaka, Shun, Fujita, Jun, Yasuda, Satoshi, Furukawa, Akemi, Hirase, Kanae, Nagai, Toshiyuki, Otsuka, Fumiyuki, Nishimura, Shigeyuki, Nakano, Shintaro, de Werf, Frans Van, Goetschalckx, Kaatje, Goetschalckx, Kaatje, Robesyn, Valerie, de Werf, Frans Van, Claes, Kathleen, White, Harvey Douglas, Alsweiler, Caroline, Hung, Chung-Lieh, Yang, Yi-Hsuan, Yun, Chun-Ho, Hou, Charles Jia-Yin, Kuo, Jen-Yuan, Yeh, Hung-I, Hung, Ta-Chuan, Li, Jiun-Yi, Chien, Chen-Yen, Tsai, Cheng-Ting, Liu, Chun-Chieh, Yu, Fa-Chang, Lin, Yueh-Hung, Lan, Wei-Ren, Yen, Chih-Hsuan, Tsai, Jui-Peng, Sung, Kuo-Tzu, Ntsekhe, Mpiko, Pandie, Shaheen, Philander (Nee Talliard), Constance, Viljoen, Charle A, Mtana, Noloyiso, De Andrade, Marianne, Maggioni, Aldo P., Moccetti, Tiziano, Anesini, Adriana, Rossi, M.Grazia, Maspoli, Simona, Mombelli, Manuela, Abdelhamid, Magdy, Talaat, Ahmed, Adel, Ahmed, Kamal, Ahmed, Mahrous, Hossam, Kaffas, Sameh El, Fishawy, Hussien El, Pop, Calin, Claudia, Matei, Popescu, Bogdan A., Ginghina, Carmen, Rosca, Monica, Deleanu, Dan, Beladan, Carmen C., Iliescu, Vlad A., Al-Mallah, Mouaz H., Zahrani, Sarah, Aljzeeri, Ahmed, Najm, Hani, Alghamdi, Ali, Mogrovejo Ramos, Walter Enrique, Monsalve Davila, Marco Antonio, White, Harvey Douglas, Alsweiler, Caroline, Kuanprasert, Srun, Mai, Chiang, Prommintikul, Arintaya, Nawarawong, Weerachai, Khwakhong, Supatchara, Woragidpoonpol, Surin, Chaiyasri, Anong, Tepsuwan, Thitipong, Mekara, Warangkana, Taksaudom, Noppon, Kulthawong, Supap, Rimsukcharoenchai, Chataroon, Amaritakomol, Anong, Euathrongchit, Juntima, Wannasopha, Yutthaphan, Yamwong, Sukit, Panpunuan, Pachara, Sritara, Piyamitr, Aramcharoen, Suthara, Meemuk, Krissada, White, Harvey Douglas, Alsweiler, Caroline, Khairuddin, Ahmad, Mokhtar, Noor Syamira, Hadi, Hafidz Abd, Basri, Nor Asiah, Yahaya, Shaiful Azmi, Yusnida, Irni, Hashim, Humayrah, Harrington, Robert, Williams, David, Alexander, Karen P., Berger, Jeffrey, Harrington, Robert, Mark, Daniel, O’Brien, Sean M., Rosenberg, Yves, Shaw, Leslee J., Ballantyne, Christie, Berman, Daniel, Beyar, Rafael, Bhargava, Balram, Buller, Chris, (Tony) Carvalho*, Antonio, Chaitman, Bernard R., Diaz, Rafael, Doerr, Rolf, Dzavik, Vladimir, Goodman, Shaun, Gosselin, Gilbert, Hachamovitch, Rory, Hamm, Christian, Held, Claes, Helm, Malte, Huber, Kurt, Jiang, Lixin, Keltai, Matyas, Kohsaka, Shun, Lang, Irene, Lopes, Renato, Lopez-Sendon, Jose, Maggioni, Aldo, Bairey Merz, C. Noel, Min, James, Peterson, Eric, Picard, Michael H., Selvanayagam, Joseph, Senior, Roxy, Sharir, Tali, Steg, Gabriel, Szwed, Hanna, de Werf, Frans Van, Weintraub, William, White, Harvey, Williams, David, Ballantyne, Christie, Calfas*, Karen, Chaitman, Bernard R., Champagne, Mary Ann, Davidson, Michael, Fleg, Jerome, McCullough, Peter A., Stone, Peter, Menasche, Philippe, Davidson*, Michael, Fremes, Stephen, Guyton, Robert, Mack, Michael, Mohr, Fred, Rao, Anupama, Sabik, Joe, Shapira, Oz, Taggart, David, Tatoulis, James, Williams, David, Blankenship, Jim, Brener, Sorin, Buller, Chris, Colombo, Antonio, Bruyne, Bernard de, Généreux, Philippe, Harrington, Robert, Kereiakes, Dean, Lefevre, Thierry, Moses, Jeffrey, Chaitman, Bernard R., Alexander, Karen P., Mahaffey, Ken, White, Harvey, Chaitman, Bernard R., Cruz-Flores, Salvador, Danchin, Nicholas, Feen, Eli, Garcia, Mario J., Hauptman, Paul, Laddu, Abhay A., Passamani, Eugene, Pina, Ileana L., Simoons, Maarten, Skali, Hicham, Thygesen, Kristian, Waters, David, Alexander, Karen P., Endsley, Patricia, Esposito, Gerard, Kanters, Jeffrey, Pownall, John, Stournaras, Dimitrios, Shaw, Leslee J., Berman, Daniel, Friedrich, Matthias, Hachamovitch, Rory, Kwong, Raymond, Min, James, Oliver, Dana, Picard, Michael H., Harrell, Frank, Blume, Jeffrey, Lee, Kerry, O’Brien, Sean M., Berger, Jeffrey, Held, Claes, Kullo, Iftikhar, McManus, Bruce, Newby, Kristin, Mark, Daniel, Cohen, David, Weintraub, William, Merz, C. Noel Bairey, Bugiardini, Raffaele, Celutkiene, Jelena, Escobedo, Jorge, Hoye, Angela, Lyubarova, Radmila, Mattina, Deirdre, Peteiro, Jesus, Alexander, Karen P., Berger, Jeffrey, Harrington, Robert, O’Brien, Sean M., Rosenberg, Yves, Mark, Daniel, Mark, Daniel, Shaw, Leslee J., Berman, Dan, Chaitman, Bernard R., Fleg, Jerome, Kwong, Raymond, Picard, Michael H., Senior, Roxy, Min, James, Leipsic, Jonathan, Ali, Ziad, Williams, David, Fleg, Jerome, Berger, Jeffrey, Chaitman, Bernard R., Alexander, Karen P., Alexander, Karen P., Fleg, Jerome, Mathew, Roy, O’Brien, Sean M., Sidhu, Mandeep, Friedman, Lawrence, Anderson, Jeffrey, Berg, Jessica, DeMets, David, Gibson, C. Michael, Lamas, Gervasio, Deming, Nicole, Himmelfarb, Jonathan, Ouyang, Pamela, Woodard, Pamela, Harrell, Frank, Nwosu, Samuel, Rosenberg, Yves, Fleg, Jerome, Kirby, Ruth, Jeffries, Neal, Berger, Jeffrey, Sidhu, Mandeep, Denaro*, Jean E., Mavromichalis, Stephanie, Chan, Kevin, Cobb, Gia, Contreras, Aira, Cukali, Diana, Ferket, Stephanie, Gabriel, Andre, Hansen, Antonietta, Roberts, Arline, Chang, Michelle, Islam, Sharder, Wayser, Graceanne, Yakubov, Solomon, Yee, Michelle, Callison, Caroline, Hogan, Isabelle, Qelaj, Albertina, Pirro, Charlotte, Loo, Kerrie Van, Wisniewski, Brianna, Gilsenan, Margaret, Lang, Bevin, Mohamed, Samaa, Esquenazi-Karonika, Shari, Mathews, Patenne, Naumova, Anna, Lyo, Jihyun, Setang, Vincent, Xavier, Mark, O’Brien, Sean M., Alexander, Karen P., Mark, Daniel B., Anstrom, Kevin, Baloch, Khaula, Blount, Janet, Cowper, Patricia, Davidson-Ray, Linda, Drew, Laura, Harding, Tina, Knight, J David, Liu, Diane Minshall, O’Neal, Betsy, Redick, Thomas, Jones, Philip, Nugent, Karen, Wang, Grace Jingyan, Shaw, Leslee J., Phillips, Lawrence, Goyal, Abhinav, Hetrick, Holly, Oliver, Dana, Berman, Daniel, Hayes, Sean W., Friedman, John D., Gerlach, R. James, Hyun, Mark, Miranda-Peats, Romalisa, Slomka, Piotr, Thomson, Louise, Kwong, Raymond Y., Friedrich, Matthias, Mongeon, Francois Pierre, Michael, Steven, Picard, Michael H., Hung, Judy, Scherrer-Crosbie, Marielle, Zeng, Xin, Chaitman, Bernard R., Eckstein, Jane, Guruge, Bandula, Streif, Mary, Ali, Ziad, Genereux, Philippe, Alfonso, Maria A., Corral, Maria P., Garcia, Javier J., Horst, Jennifer, Jankovic, Ivana, Konigstein, Maayan, Lustre, Mitchel B., Peralta, Yolayfi, Sanchez, Raquel, Min, James, Arsanjani, Reza, Budoff, Matthew, Elmore, Kimberly, Gomez, Millie, Hague, Cameron, Hindoyan, Niree, Leipsic, Jonathan, Nakanishi, Rine, Srichai-Parsia, M. Barbara, Yeoh, Eunice, Youn, Tricia, Maggioni, Aldo P., Bianchini, Francesca, Ceseri, Martina, Lorimer, Andrea, Magnoni, Marco, Orso, Francesco, Sarti, Laura, Tricoli, Martinia, Carvalho, Antonio, Lopes, Renato, Barbosa, Lilian Mazza, Duarte, Tauane Bello, Soares, Tamara Colaiácovo, Aveiro Morata, Julia de, Carvalho, Pedro, Carvalho Maffei, Natalia de, Egydio, Flávia, Kawakami, Anelise, Oliveira, Janaina, Piloto, Elissa Restelli, Pozzibon, Jaqueline, Goodman, Shaun, Camara, Diane, Mowafy, Neamat, Spindler, Caroline, Jiang, Lixin, Dai, Hao, Feng, Fang, Li, Jia, Li, Li, Liu, Jiamin, Xie, Qiulan, Zhang, Haibo, Zhang, Jianxin, Zhang, Lihua, Zhang, Liping, Zhang, Ning, Zhong, Hui, Diaz, Rafael, Escobar, Claudia, Martin, Maria Eugenia, Pascual, Andrea, Lopez-Sendon, José, Moraga, Paloma, Hernandez, Victoria, Castro, Almudena, Posada, Maria, Fernandez, Sara, Villanueva, José Luis Narro, Selgas, Rafael, Steg, Gabriel, Abergel, Helene, Juliard, Jean Michel, White, Harvey, Alsweiler, Caroline, de Werf, Frans Van, Claes, Kathleen, Goetschalckx, Kaatje, Luyten, Ann, Robesyn, Valerie, Selvanayagam, Joseph B., Murphy, Deirdre, Ahmed, Asker, Bhatt, Richa, Chadha, Nitika, Kumar, Vijay, Lubna, Sadath, Naik, Pushpa, Pandey, Shruti, Ramasamy, Karthik, Saleem, Mohammed, Sharma, Pratiksha, and Siddaram, Hemalata

Published
2024
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17. Stability analysis of time-delay systems in the parametric space

Author

Turkulov, Vukan, Rapaic, Milan R., and Malti, Rachid

Subjects
Electrical Engineering and Systems Science - Systems and Control
Abstract

This paper presents a novel method for stability analysis of a wide class of linear, time-delay systems (TDS), including retarded non-neutral ones, as well as those incorporating incommensurate and distributed delays. The proposed method is based on frequency domain analysis and the application of Rouche's theorem. Given a parametrized TDS, and some parametric point for which the number of unstable poles is known, the proposed method is capable of identifying the maximum surrounding region in the parametric space for which the number of unstable poles remains invariant. First, a procedure for investigating stability along a line is developed. Then, the results are extended by the application of Holder's inequality to investigating stability within a region. Contrary to existing approaches, the proposed method is uniformly applicable to parameters of different types (delays, distributed delay limits, time constants, etc.). Efficacy of the proposed method is demonstrated using illustrative examples., Comment: 11 pages, 6 figures, submitted to Automatica

Published
2021

18. Amide–π Interactions in the Structural Stability of Proteins: Role in the Oligomeric Phycocyanins

Author

Luka M. Breberina, Mario V. Zlatović, Srđan Đ. Stojanović, and Milan R. Nikolić

Subjects
phycocyanins, interfaces, amide–π interactions, stabilization centers, hot-spot regions, ab initio method, Electronic computers. Computer science, QA75.5-76.95
Abstract

This study investigates the influences and environmental preferences of amide–π interactions, a relatively unexplored class of charge-free interactions, in oligomeric phycocyanins. In a data set of 20 proteins, we observed 2086 amide–π interactions, all of which were part of the protein backbone. Phe and Tyr residues were found to be involved in amide–π interactions more frequently than Trp or His. The most favorable amide–π interactions occurred within a pair distance range of 5–7 Å, with a distinct angle preference for T-shaped ring arrangements. Multiple interaction patterns suggest that approximately 76% of the total interacting residues participate in multiple amide–π interactions. Our ab initio calculations revealed that most amide–π interactions have energy from 0 to −2 kcal/mol. Stabilization centers of phycocyanins showed that all residues in amide–π interactions play a crucial role in locating one or more such centers. Around 78% of the total interacting residues in the dataset contribute to creating hot-spot regions. Notably, the amide–π interacting residues were found to be highly evolutionarily conserved. These findings enhance our understanding of the structural stability and potential for protein engineering of phycocyanins used as bioactive natural colorants in various industries, including food and pharmaceuticals.

Published
2024
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22. Role of Duodenal Biopsy in the Diagnosis of Giardiasis with Negative Stool Tests: A Case Report and Literature Review

Author

Milan R Akbari, Nirali V Vagadiya, Rajeshkumar R Akbari, Roshani B Sanghani, Manali Chodvadiya, and Keval A Patel

Subjects
duodenal biopsy, endoscopy, giardia lamblia, giardiasis, malabsorption, Medicine (General), R5-920
Abstract

Giardia lamblia (also known as Giardia intestinalis and Giardia duodenalis) is the most frequent intestinal parasite responsible for chronic diarrhea and malnutrition. Giardia infections commonly cause nausea, abdominal cramping, bloating, and foul-smelling diarrhea in patients. Usually, Giardiasis causes a self-limiting illness, but it can progress to a severe disease in immunocompromised individuals and cause dehydration, malnutrition, and failure to thrive. As a result, early diagnosis and treatment are required to control the infection and prevent complications. Infectious Disease Society of America diagnostic guidelines recommend obtaining stool studies to diagnose Giardiasis. When stool tests are negative, but suspicion persists, a duodenal biopsy is a gold standard for diagnosis. We present the case of a patient diagnosed with Giardia by an incidental duodenal biopsy specimen obtained during a workup for malnutrition and chronic diarrhea, despite a normal stool examination. A few cases of Giardiasis have been diagnosed and reported in the literature using a duodenal biopsy. Some studies addressed the same issue, and we believe that duodenal biopsy can be a good strategy for diagnosing Giardiasis with high sensitivity and specificity.

Published
2023
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24. GOT1 inhibition promotes pancreatic cancer cell death by ferroptosis

Author

Kremer, Daniel M, Nelson, Barbara S, Lin, Lin, Yarosz, Emily L, Halbrook, Christopher J, Kerk, Samuel A, Sajjakulnukit, Peter, Myers, Amy, Thurston, Galloway, Hou, Sean W, Carpenter, Eileen S, Andren, Anthony C, Nwosu, Zeribe C, Cusmano, Nicholas, Wisner, Stephanie, Mbah, Nneka E, Shan, Mengrou, Das, Nupur K, Magnuson, Brian, Little, Andrew C, Savani, Milan R, Ramos, Johanna, Gao, Tina, Sastra, Stephen A, Palermo, Carmine F, Badgley, Michael A, Zhang, Li, Asara, John M, McBrayer, Samuel K, di Magliano, Marina Pasca, Crawford, Howard C, Shah, Yatrik M, Olive, Kenneth P, and Lyssiotis, Costas A

Subjects
Biochemistry and Cell Biology, Biomedical and Clinical Sciences, Oncology and Carcinogenesis, Biological Sciences, Rare Diseases, Digestive Diseases, Cancer, Pancreatic Cancer, 2.1 Biological and endogenous factors, Aetiology, Animals, Antioxidants, Aspartate Aminotransferase, Cytoplasmic, Cell Line, Tumor, Cell Proliferation, Cell Survival, Cystine, Ferroptosis, Glutathione, Humans, Iron, Mice, Mitochondria, Pancreatic Neoplasms
Abstract

Cancer metabolism is rewired to support cell survival in response to intrinsic and environmental stressors. Identification of strategies to target these adaptions is an area of active research. We previously described a cytosolic aspartate aminotransaminase (GOT1)-driven pathway in pancreatic cancer used to maintain redox balance. Here, we sought to identify metabolic dependencies following GOT1 inhibition to exploit this feature of pancreatic cancer and to provide additional insight into regulation of redox metabolism. Using pharmacological methods, we identify cysteine, glutathione, and lipid antioxidant function as metabolic vulnerabilities following GOT1 withdrawal. We demonstrate that targeting any of these pathways triggers ferroptosis, an oxidative, iron-dependent form of cell death, in GOT1 knockdown cells. Mechanistically, we reveal that GOT1 inhibition represses mitochondrial metabolism and promotes a catabolic state. Consequently, we find that this enhances labile iron availability through autophagy, which potentiates the activity of ferroptotic stimuli. Overall, our study identifies a biochemical connection between GOT1, iron regulation, and ferroptosis.

Published
2021

27. Project-Based Learning of Diffusion and Osmosis: Opinions of Students of Physics and Technology at University of Novi Sad

Author

Cavic, Milan R., Stanisavljevic, Jelena D., Bogdanovic, Ivana Z., Skuban, Sonja J., and Pavkov-Hrvojevic, Milica V.

Abstract

There are subjects which university students perceive as uninteresting and which they are reluctant to learn. The use of an appropriate approach to learning can contribute to the formation of positive students' opinions on learning. Project-based learning (PjBL) is characterized by active research, problem-solving, and student-made projects which is nowdays usually facilitated by the use of computer and network technologies. The aim of this research is to assess opinions of students of physics and technology at University of Novi Sad, Republic of Serbia, on PjBL, as well as to analyze these opinions in connection to three different factors: gender, academic performance, and study program. The physics content "Diffusion and Osmosis" was realized using PjBL. This topic was chosen because of possible interdisciplinary concepts' relations between physics, chemistry, and biology. After students' group work on projects and their project reports, a survey was conducted. Research results showed that opinions of students participating in the research about PjBL were independent of their gender, academic performance, as well as whether they study physics or technology. All students had equally positive opinions on PjBL. Further implementation of PjBL is planned in the authors' departments, along with the integrative implementation of PjBL in teaching science in collaboration with other departments at the university.

Published
2022
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31. Autologous fibroblasts for vocal scars and age‐related atrophy: A randomized clinical trial

Author

Ma, Yue, Long, Jennifer, Amin, Milan R, Branski, Ryan C, Damrose, Edward J, Sung, Chih‐Kwang, Achlatis, Stratos, Kearney, Ann, and Chhetri, Dinesh K

Subjects
Biomedical and Clinical Sciences, Clinical Sciences, Clinical Research, Clinical Trials and Supportive Activities, Evaluation of treatments and therapeutic interventions, 6.1 Pharmaceuticals, Adolescent, Adult, Aging, Atrophy, Cicatrix, Double-Blind Method, Dysphonia, Female, Fibroblasts, Humans, Injections, Laryngeal Diseases, Male, Middle Aged, Transplantation, Autologous, Treatment Outcome, Vocal Cords, Young Adult, Randomized clinical trial, vocal folds scar, vocal fold, voice, presbyphonia, vocal fold atrophy, autologous fibroblasts, voice quality, Otorhinolaryngology, Clinical sciences
Abstract

Objectives/hypothesisTo assess the safety and efficacy of autologous cultured fibroblasts (ACFs) to treat dysphonia related to vocal fold scar and age-related vocal atrophy (ARVA).Study designRandomized, double-blinded, placebo-controlled, multi-institutional, phase II trial.MethodsACFs were expanded from punch biopsies of the postauricular skin in each subject; randomization was 2:1 (treatment vs. placebo). Three injections of 1-2 × 107 cells or placebo saline was performed at 4-week intervals for each vocal fold. Follow-up was performed at 4, 8, and 12 months. The primary outcome was improved mucosal waves. Secondary outcomes included Voice Handicap Index (VHI)-30, patient reported voice quality outcomes, and perceptual analysis of voice.ResultsFifteen subjects received ACF and six received saline injections. At 4, 8, and 12 months after ACF treatments, a significant improvement in mucosal wave grade relative to baseline was observed in both vocal scar and ARVA groups. Relative to control group, mucosal waves were significantly improved in the ARVA group at 4 and 8 months. Perceptual analysis significantly improved in the vocal scar group 12 months after ACF treatments compared to controls. Vocal scar group reported significantly improved vocal quality from baseline. VHI and expert rater voice grade improved in both groups, but did not achieve significance. No adverse events related to fibroblast injections were observed.ConclusionsIn this cohort, injection of ACFs into the vocal fold lamina propria (LP) was safe and significantly improved mucosal waves in patients with vocal scar and ARVA. ACF may hold promise to reconstruct the LP.Level of evidence1 Laryngoscope, 130:2650-2658, 2020.

Published
2020

32. On the importance of π-π interactions in the structural stability of phycocyanins

Author

Breberina Luka M., Nikolić Milan R., Stojanović Srđan Đ., and Zlatović Mario V.

Subjects
phycobiliproteins, aromatic interactions, stabilization centers, aminoacid conservation, ab initio study, Chemistry, QD1-999
Abstract

The influences of π-π interactions in phycocyanin proteins and their environmental preferences were analyzed. The observations indicate that the majority of the aromatic residues in phycocyanin proteins are involved in π-π interactions. Phenylalanine (Phe) and tyrosine (Tyr) residues were found to be involved in π–π interactions much more frequently than tryptophan (Trp) or histidine (His). Similarly, the Phe-Phe and Tyr-Tyr π-π interacting pair had the highest frequency of occurrence. In addition to π-π interactions, the aromatic residues also form π-networks in phycocyanins. The π–π interactions are most favourable at the pair distance range of 5.5–7 Å, with a clear preference for T-shaped ring arrangements. Using ab initio calculations, we observed that most of the π-π interactions possess energy from 0 to -10kJ mol-1. Stabilization centres for these proteins showed that all residues found in π-π interactions are important in locating one or more such centres. Π-π interacting residues are evolutionary conserved. The results obtained from this study will be beneficial in further understanding the structural stability and eventual development of protein engineering of phycocyanins.

Published
2023
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33. Radnička (ne)moć u automobilskoj industriji u Srbiji: fabrički režimi rada i razgradnja strukturne moći radništva

Author

Škobić, Milan R., Škobić, Milan R., Škobić, Milan R., and Škobić, Milan R.

Abstract

Rad pokušava eksplorativno da osvetli nemoć radništva u pogledu promene sopstvenog lošeg položaja u automobilskoj industriji u Srbiji. U radu se tvrdi da sklop odnosa između podružnica, centrala, klijenata i dobavljača u industriji generiše određene elemente režima rada u pojedinim fabrikama koje smanjuju, sakrivaju i fragmentišu strukturnu moć radništva. U tim okolnostima, poslovodstvo može da primenjuje tehnike intenziviranja proizvodnje i učestalog oslanjanja na prekovremeni rad bez otpora radništva. Primenom koncepata teorije resursa moći, režima rada, globalnih proizvodnih mreža, i globalnih lanaca vrednosti, u radu se situira odnos između različitih preduzeća koja čine tu industriju, i radništva u Srbiji. Konkretni elementi režima rada kojim se postižu ovi efekti zavise od pozicije firme u automobilskoj industriji i njene poslovne strategije, što dovodi do heterogene situacije unutar i između fabrika analiziranih u ovom radu. Organizacija proizvodnje dovodi i do mogućnosti rasta, otkrivanja, i ukrupnjavanja latentne ili neiskorišćene strukturne moći, i u radu su identifikovane neke njene instance. Na osnovu te analize, u zaključku su ponuđene smernice za dalje istraživanje koje se tiču povezivanja analize industrije sa njenim širim socijalnim okruženjem, preciznije i sistematičnije analize same industrije, i položaja i uloge sindikata u odnosu između rada i kapitala u automobilskoj industriji u Srbiji., Workers’ rights are systematically violated in Serbia. On the other hand, the perception of these violations does not lead to sustained attempts at building workers’ power that could resist these violations and change their working conditions. This paper explores the industry-engendered conditions that inhibit the building of workers’ power by looking at four automotive subsidiaries of foreign companies in Serbia. This is done by identifying several ways in which the relationships between subsidiaries, headquarters, clients, and suppliers inhibit sources of workers’ power, in particular their structural power. The paper employs the conceptualization of sources of workers’ power developed in the tradition of power resource theory: structural, associational, institutional, and societal. In particular, the focus is on the structural source of power as it is most closely related to the point of production: structural power refers to the power that can be generated by the position of workers in the labor market, and by their position in the organization of production - within particular factories and the network of relations between different companies in the industry. Four automotive subsidiaries of foreign investors in Serbia are analyzed to identify labor regimes they set up, and the elements of those labor regimes that are constituted in relationship with the headquarters, their clients, and suppliers. Labor regimes refer to the wider social set-up in which the relationship of exploitation is embedded. These labor regimes, and the inter-firm relationships that engender the elements of those regimes, constitute the strategic environment in which workers’ power would be built, and engender the problems for various segments of the workforce that building workers’ power could address. These four subsidiaries differ in their position in the global production networks of the automotive industry and are caught up in different governance patterns as conceptualized by the glo

Published
2024

37. Water: new aspect of hydrogen bonding in the solid state

Author

Milan R. Milovanović, Ivana M. Stanković, Jelena M. Živković, Dragan B. Ninković, Michael B. Hall, and Snežana D. Zarić

Subjects
water, hydrogen bonds, antiparallel interactions, ab initio calculations, Crystallography, QD901-999
Abstract

All water–water contacts in the crystal structures from the Cambridge Structural Database with dOO ≤ 4.0 Å have been found. These contacts were analysed on the basis of their geometries and interaction energies from CCSD(T)/CBS calculations. The results show 6729 attractive water–water contacts, of which 4717 are classical hydrogen bonds (dOH ≤ 3.0 Å and α ≥ 120°) with most being stronger than −3.3 kcal mol−1. Beyond the region of these hydrogen bonds, there is a large number of attractive interactions (2062). The majority are antiparallel dipolar interactions, where the O—H bonds of two water molecules lying in parallel planes are oriented antiparallel to each other. Developing geometric criteria for these antiparallel dipoles (β1, β2 ≥ 160°, 80 ≤ α ≤ 140° and THOHO > 40°) yielded 1282 attractive contacts. The interaction energies of these antiparallel oriented water molecules are up to −4.7 kcal mol−1, while most of the contacts have interaction energies in the range −0.9 to −2.1 kcal mol−1. This study suggests that the geometric criteria for defining attractive water–water interactions should be broader than the classical hydrogen-bonding criteria, a change that may reveal undiscovered and unappreciated interactions controlling molecular structure and chemistry.

Published
2022
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40. Factors associated with adherence to swallowing therapy among patients diagnosed with oropharyngeal dysphagia.

Author

Ezeh, Uche C., Balou, Matina, Crosby, Tyler, Kwak, Paul E., and Amin, Milan R.

Subjects
PATIENT compliance, RACE, MARITAL status, LOGISTIC regression analysis, NATIVE language, DEGLUTITION, FISHER exact test, CHI-squared test
Abstract

Objective: The objective of this study is to assess disparities in adherence to swallowing therapy for clinically diagnosed oropharyngeal dysphagia (OD) patients. Methods: Analysis was conducted on data from 600 patients with OD and confirmed impairments in swallowing safety and/or efficiency on a videofluoroscopic swallow study. Patients were classified based on their adherence to treatment sessions, defined as the number of swallow treatment sessions attended. The outcome of treatment adherence was categorized into two groups: those who attended fewer than 50% of the prescribed treatment sessions and those who attended 50% or more of the sessions. Continuous variables were presented as mean ± standard deviation or median ± interquartile range. Categorical variables were compared using Pearson chi‐square tests and Fisher's exact test when appropriate. Univariable and multivariable binary logistic regression models were employed to identify factors associated with successful adherence. Results: Approximately 79% adhered to swallowing treatment. We found no significant relationship between adherence and age, sex, race, ethnicity, primary language, marital status, insurance status, occupation, median income, distance, education, OD severity, and diagnosis year (p > 0.05). We found no covariables to be significant predictors to swallowing treatment nonadherence in both univariable and multivariable binary regression models (p > 0.05). Conclusion: The variables analyzed in this study were not significantly associated with nonadherence to swallow therapy. Nevertheless, our study still addressed an important knowledge gap and future studies would benefit from exploring other relevant socioeconomic and disease‐related factors. Level of evidence: Level 4. [ABSTRACT FROM AUTHOR]

Published
2024
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42. On some important quantities influencing proper functioning of the differential pneumatic comparator

Author

Burazer Jela M., Skoko Dragiša M., Novković Đorđe M., Lečić Milan R., Vorotović Goran S., and Januzović Miloš B.

Subjects
back pressure air gauge, pressure, nozzle head fouling, flapper-nozzle area width, tolerance field, Engineering (General). Civil engineering (General), TA1-2040, Mechanics of engineering. Applied mechanics, TA349-359
Abstract

Back-pressure air gauging is an effective and practical way of controlling machine parts in large-scale production. It is a non-contact measuring technique based on the flapper-nozzle effect. The proper functioning of a differential pneumatic comparator depends on several geometric parameters as well as flow conditions inside the device. The main problems of this controlling technique are the fouling of the measuring nozzle head and changes in the accuracy of the comparator. This paper examines the influence of the supply pressure, the diameter of the orifice in the measuring branch, and the axial distance in the flapper-nozzle area on pneumatic comparator performance. In a way, we are trying to optimize the performance of a given pneumatic comparator with respect to the tolerance field for which it is intended. The size, strength, and position of the vacuum in the flapper-nozzle area depend on the supply pressure and the axial distance between the measuring nozzle outlet cross-section and the workpiece surface. For a certain combination of these two parameters, we can influence the vacuum quantities. A pneumatic sensitivity of a comparator can be increased by increasing the supply pressure. The greater accuracy of the back pressure air gauge, the smaller the application range, i.e. the tolerance field that we can control with a given device.

Published
2022
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43. Long-term follow-up of patients with chronic total coronary artery occlusion previously randomized to treatment with optimal drug therapy or percutaneous revascularization of chronic total occlusion (COMET-CTO)

Author

Stefan A. Juricic, Sinisa M. Stojkovic, Alfredo R. Galassi, Goran R. Stankovic, Dejan N. Orlic, Vladan D. Vukcevic, Dejan G. Milasinovic, Srdjan B. Aleksandric, Miloje V. Tomasevic, Milan R. Dobric, Milan A. Nedeljkovic, Branko D. Beleslin, Miodrag P. Dikic, Marko D. Banovic, Miodrag C. Ostojic, and Milorad B. Tesic

Subjects
percutaneous coronary intervention, chronic total occlusion, optimal medical therapy, outcomes, long-term follow-up, Diseases of the circulatory (Cardiovascular) system, RC666-701
Abstract

BackgroundThe COMET-CTO trial was a randomized prospective study that assessed long-term follow-up in patients with chronic total occlusion (CTO) in coronary arteries treated with percutaneous coronary intervention (PCI) or with optimal medical therapy (OMT). During the 9-month follow-up, the incidence of major adverse cardiac events (MACE) did not differ between the two groups; no death or myocardial infarction (MI) was observed. There was a significant difference in quality of life (QoL), assessed by the Seattle Angina Questionnaire (SAQ), in favor of the PCI group. Here we report long-term follow-up results (56 ± 12 months).MethodsBetween October 2015 and May 2017, a total of 100 patients with CTO were randomized into two groups of 50 patients: PCI CTO or OMT group. The primary endpoint of the current study was the incidence of MACE defined as cardiac death, MI, and revascularization [PCI or coronary artery bypass graft (CABG)]. As the secondary exploratory outcome, we analyzed all the cause-mortality rate.ResultsOut of 100 randomized patients, 92 were available for long-term follow-up (44 in the PCI group and 48 in the OMT group). The incidence of MACE did not differ significantly between the two groups (p = 0.363). Individual components of MACE were distributed, respectively: cardiac death (OMT vs. PCI group, 6 vs. 3, p = 0.489), MI (OMT vs. PCI group, 1 vs. 0, p = 1), and revascularization (PCI: OMT vs. PCI group, 2 vs. 2, p = 1; CABG: OMT vs. PCI group, 1 vs. 1, p = 1). There was no significant difference between the two groups regarding the individual component of MACE. Six patients died from non-cardiac causes [five deaths were reported in the OMT group and one death in the PCI group (p = 0.206)]. Kaplan-Meier survival curves for MACE did not differ significantly between the study groups (log-rank 0.804, p = 0.370). Regarding the secondary exploratory outcome, a total of 15 patients died at 56 ± 12 months (11 in the OMT and 4 in the PCI group) (p = 0.093). The Kaplan-Meier survival curves for all-cause mortality rates did not differ significantly between the two groups (log rank 3.404, p = 0.065). There were no statistically significant differences between OMT and PCI groups in all five SAQ domains. There was a significant improvement in three SAQ domains in the PCI group: PL (p < 0.001), AF (p = 0.007), and QoL (p = 0.001).ConclusionAfter 56 ± 12 months of follow-up, the incidence of MACE, as well as QoL measured by SAQ, did not differ significantly between the PCI and OMT groups.

Published
2023
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46. Experimental evaluation of protection and immunogenicity of Streptococcus suis bacterin-based vaccines formulated with different commercial adjuvants in weaned piglets

Author

Milan R. Obradovic, Lorelei Corsaut, Dominic Dolbec, Marcelo Gottschalk, and Mariela Segura

Subjects
Streptococcus suis, swine, bacterin vaccines, adjuvants, antibodies, Alhydrogel®, Veterinary medicine, SF600-1100
Abstract

Abstract Streptococcus suis is an important swine pathogen responsible for economic losses to the swine industry worldwide. There is no effective commercial vaccine against S. suis. The use of autogenous (“bacterin”) vaccines to control S. suis outbreaks is a frequent preventive measure in the field, although scientific data on immunogenicity and reduction in mortality and morbidity are scarce. The goal of our study is to experimentally evaluate the immunogenicity and protective efficacy against homologous challenge in weaned piglets of a S. suis serotype 2 bacterin-based vaccine formulated with six different commercial adjuvants (Alhydrogel®, Emulsigen®-D, Quil-A®, Montanide™ ISA 206 VG, Montanide™ ISA 61 VG, and Montanide™ ISA 201 VG). The vaccine formulated with Montanide™ ISA 61 VG induced a significant increase in anti-S. suis antibodies, including both IgG1 and IgG2 subclasses, protected against mortality and significantly reduced morbidity and severity of clinical signs. Vaccines formulated with Montanide ISA 206 VG or Montanide ISA 201 VG also induced a significant increase in anti-S. suis antibodies and showed partial protection and reduction of clinical signs severity. Vaccines formulated with Alhydrogel®, Emulsigen®-D, or Quil-A® induced a low and IgG1-shifted antibody response and failed to protect vaccinated piglets against a homologous challenge. In conclusion, the type of adjuvant used in the vaccine formulation significantly influenced the immune response and efficacy of the vaccine against a homologous challenge.

Published
2021
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49. Covalent Conjugates of Allylbenzenes and Terpenoids as Antibiotics Enhancers with the Function of Prolonged Action

Author

Igor D. Zlotnikov, Maria P. Davydova, Milan R. Danilov, Sergey S. Krylov, Natalya G. Belogurova, and Elena V. Kudryashova

Subjects
covalent antibacterial conjugate, moxifloxacin, limonene, prolonged pharmacokinetic, FTIR spectroscopy, Medicine, Pharmacy and materia medica, RS1-441
Abstract

The drug resistance of pathogenic bacteria is often due efflux pumps—specific proteins that remove foreign compounds from bacterial cells. To overcome drug resistance, adjuvants are often used that can inhibit efflux pumps or other systems that ensure the resistance of bacteria to the action of antibiotics. We assumed that a new level of effectiveness with the use of an antibiotic + an adjuvant pair could be achieved by their joint delivery into the pathogen. To test this hypothesis, we constructed a series of molecular carriers based on poly-(olygo-, dendry)mers based on cyclodextrin-grafted PEI or mannan, as well as glycol chitosan, covalently bound to antibiotic, adjuvant, and the oligosaccharide ligand to the macrophage mannose receptor (CD206), which we studied earlier and showed high efficiency and selectivity of delivery of a therapeutic “cargo” to macrophages. Moxifloxacin was used as an antibiotic, and terpenoid and allylbenzene compounds were used as adjuvants, for which we previously discovered the ability to inhibit bacterial efflux pumps. We show that: (a) the resulting structures were stable in vitro for a long time (up to 10 days); (b) they were adsorbed on bacterial cells, providing a local increase in the concentration of the antibiotic and adjuvant in pathogen cells; (c) they were internalized by bacterial cells, ensuring the accumulation of both antibiotic and adjuvant inside bacterial cells; (d) the adjuvant, after entering the bacterial cell, provided inhibition of the efflux pumps; (e) due to this action of the adjuvant, combined with the targeted delivery by the carrier, the antibiotic’s half-life in rats increased by more than 2 times, the effective concentration of the drug in the blood plasma (AUC) increased up to 8–10 times; (f) a significant increase in the effectiveness of the antibacterial action against Gram+ and Gram- cells was achieved (up to 3 times). Potentially, such an approach would significantly increase the effectiveness of therapies for a number of infectious and other diseases, reduce the dosage of antibiotics, shorten the duration of treatment, and reduce the risk of developing bacterial resistance. Moreover, the use of a polymer carrier with covalently bound organic molecules of different structures will avoid problems linked to different (suboptimal) solubility and bio-distribution of the administered molecules, which would be almost inevitable when using the same compounds separately. It would be very difficult to find antibiotic/adjuvant pairs that simultaneously achieve optimal concentrations in the same target cells. In our case, terpenoids and alkylbenzenes used as adjuvants are practically insoluble as individual compounds, and their unacceptable pharmacological properties would not allow them to be used as efflux pump inhibitors.

Published
2023
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50. Can Technology Reinforce Cogency of the Architectural Argument: Trial and Error Approach

Author

Jelena Atanacković Jeličić, Milan R. Rapaić, Igor Maraš, Erne Tot, and Dejan Ecet

Subjects
transdisciplinary urban planning, design process methodology, design strategy vs. design tactics, agile development, Scrum framework, project management efficiency, Building construction, TH1-9745
Abstract

The main question proposed in this research is whether different types of organizational approaches could help in shortening the response time needed to analyze advanced design solutions in accordance with the changed circumstances. Approaches that we are considering have been adapted from rapidly changing disciplines—such as the IT industry, and software engineering. This paradigm allows for architectural programming to obtain different positions in the timeline of project planning and realization. We proposed a novel methodology of architectural design and project management as an instrument inspired by the Agile Manifesto and some of its instantiations, most notably by the Scrum framework. This research shows that application of the proposed framework significantly shortens the design process and facilitates the involvement of a larger number of authors within the same project team. This study focused on the specific case of architectural competitions. However, the results showed that the same framework could be applied in a broader design context, details of which have been left for future considerations.

Published
2023
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