Search

Your search keyword '"Mijnheer, Gerdien"' showing total 33 results
Start Over Author "Mijnheer, Gerdien"
33 results on '"Mijnheer, Gerdien"'

1. Conserved human effector Treg cell transcriptomic and epigenetic signature in arthritic joint inflammation

Author

Mijnheer, Gerdien, Lutter, Lisanne, Mokry, Michal, van der Wal, Marlot, Scholman, Rianne, Fleskens, Veerle, Pandit, Aridaman, Tao, Weiyang, Wekking, Mark, Vervoort, Stephin, Roberts, Ceri, Petrelli, Alessandra, Peeters, Janneke G. C., Knijff, Marthe, de Roock, Sytze, Vastert, Sebastiaan, Taams, Leonie S., van Loosdregt, Jorg, and van Wijk, Femke

Published
2021
Full Text
View/download PDF

2. [CD8.sup.+] T cells are clonally expanding effectors in human chronic inflammation

Author

Petrelli, Alessandra, Mijnheer, Gerdien, van Konijnenburg, David P. Hoytema, van der Wal, Maria M., Giovannone, Barbara, Mocholi, Enric, Vazirpanah, Nadia, Broen, Jasper C., Hijnen, Dirkjan, Oldenburg, Bas, Coffer, Paul J., Vastert, Sebastian J., Prakken, Berent J., Spierings, Eric, Pandit, Aridaman, Mokry, Michal, and van Wijk, Femke

Subjects
CD8 lymphocytes -- Health aspects, Inflammation -- Genetic aspects -- Health aspects, Health care industry
Abstract

Chronic inflammatory diseases are characterized by recurrent inflammatory attacks in the tissues mediated by autoreactive T cells. Identity and functional programming of [CD8.sup.+] T cells at the target site of inflammation still remain elusive. One key question is whether, in these antigen-rich environments, chronic stimulation leads to [CD8.sup.+] T cell exhaustion comparable to what is observed in infectious disease contexts. In the synovial fluid (SF) of juvenile idiopathic arthritis (JIA) patients, a model of chronic inflammation, an overrepresentation of PD-V[CD8.sup.+] T cells was found. Gene expression profiling, gene set enrichment analysis, functional studies, and extracellular flux analysis identified [PD-1.sup.+][CD8.sup.+] T cells as metabolically active effectors, with no sign of exhaustion. Furthermore, [PD-1.sup.+] [CD8.sup.+] T cells were enriched for a tissue-resident memory (Trm) cell transcriptional profile and demonstrated increased clonal expansion compared with the [PD-1.sup.-] counterpart, suggesting antigen-driven expansion of locally adapted cells. Interestingly, this subset was also found increased in target tissues in other human chronic inflammatory diseases. These data indicate that local chronic inflammation drives the induction and expansion of [CD8.sup.+] T cells endowed with potential detrimental properties. Together, these findings lay the basis for investigation of PD-1-expressing [CD8.sup.+] T cell targeting strategies in human chronic inflammatory diseases., Introduction Chronic inflammatory diseases, such as inflammatory bowel disease (IBD), juvenile idiopathic arthritis (JIA), and atopic dermatitis (AD), are heterogeneous clinical disorders representing a major public health issue (1). Current [...]

Published
2018
Full Text
View/download PDF

3. Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

Author

Mijnheer, Gerdien, Servaas, Nila Hendrika, Leong, Jing Yao, Boltjes, Arjan, Spierings, Eric, Chen, Phyllis, Lai, Liyun, Petrelli, Alessandra, Vastert, Sebastiaan, de Boer, Rob J, Albani, Salvatore, Pandit, Aridaman, van Wijk, Femke, Mijnheer, Gerdien, Servaas, Nila Hendrika, Leong, Jing Yao, Boltjes, Arjan, Spierings, Eric, Chen, Phyllis, Lai, Liyun, Petrelli, Alessandra, Vastert, Sebastiaan, de Boer, Rob J, Albani, Salvatore, Pandit, Aridaman, and van Wijk, Femke

Abstract

Autoimmune inflammation is characterized by tissue infiltration and expansion of antigen-specific T cells. Although this inflammation is often limited to specific target tissues, it remains yet to be explored whether distinct affected sites are infiltrated with the same, persistent T cell clones. Here, we performed CyTOF analysis and T cell receptor (TCR) sequencing to study immune cell composition and (hyper-)expansion of circulating and joint-derived Tregs and non-Tregs in juvenile idiopathic arthritis (JIA). We studied different joints affected at the same time, as well as over the course of relapsing-remitting disease. We found that the composition and functional characteristics of immune infiltrates are strikingly similar between joints within one patient, and observed a strong overlap between dominant T cell clones, especially Treg, of which some could also be detected in circulation and persisted over the course of relapsing-remitting disease. Moreover, these T cell clones were characterized by a high degree of sequence similarity, indicating the presence of TCR clusters responding to the same antigens. These data suggest that in localized autoimmune disease, there is autoantigen-driven expansion of both Teffector and Treg clones that are highly persistent and are (re)circulating. These dominant clones might represent interesting therapeutic targets.

Published
2023

4. Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

Author

Theoretical Biology and Bioinformatics, Sub Theoretical Biology, Mijnheer, Gerdien, Servaas, Nila Hendrika, Leong, Jing Yao, Boltjes, Arjan, Spierings, Eric, Chen, Phyllis, Lai, Liyun, Petrelli, Alessandra, Vastert, Sebastiaan, de Boer, Rob J, Albani, Salvatore, Pandit, Aridaman, van Wijk, Femke, Theoretical Biology and Bioinformatics, Sub Theoretical Biology, Mijnheer, Gerdien, Servaas, Nila Hendrika, Leong, Jing Yao, Boltjes, Arjan, Spierings, Eric, Chen, Phyllis, Lai, Liyun, Petrelli, Alessandra, Vastert, Sebastiaan, de Boer, Rob J, Albani, Salvatore, Pandit, Aridaman, and van Wijk, Femke

Published
2023

5. Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

Author

CTI Van Wijk, CTI Meyaard, CDL Cluster Onderzoek en Onderwijs, CDL Cluster Speciële Diagnostiek, Cancer, Infection & Immunity, CTI Van Loosdregt, Immuno/reuma onderzoek 1 (Vastert), Immunologie/Reumatologie, Child Health, Mijnheer, Gerdien, Servaas, Nila Hendrika, Leong, Jing Yao, Boltjes, Arjan, Spierings, Eric, Chen, Phyllis, Lai, Liyun, Petrelli, Alessandra, Vastert, Sebastiaan, de Boer, Rob J, Albani, Salvatore, Pandit, Aridaman, van Wijk, Femke, CTI Van Wijk, CTI Meyaard, CDL Cluster Onderzoek en Onderwijs, CDL Cluster Speciële Diagnostiek, Cancer, Infection & Immunity, CTI Van Loosdregt, Immuno/reuma onderzoek 1 (Vastert), Immunologie/Reumatologie, Child Health, Mijnheer, Gerdien, Servaas, Nila Hendrika, Leong, Jing Yao, Boltjes, Arjan, Spierings, Eric, Chen, Phyllis, Lai, Liyun, Petrelli, Alessandra, Vastert, Sebastiaan, de Boer, Rob J, Albani, Salvatore, Pandit, Aridaman, and van Wijk, Femke

Published
2023

7. Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

Author

Mijnheer, Gerdien, primary, Servaas, Nila Hendrika, additional, Leong, Jing Yao, additional, Boltjes, Arjan, additional, Spierings, Eric, additional, Chen, Phyllis, additional, Lai, Liyun, additional, Petrelli, Alessandra, additional, Vastert, Sebastiaan, additional, de Boer, Rob J, additional, Albani, Salvatore, additional, Pandit, Aridaman, additional, and van Wijk, Femke, additional

Published
2023
Full Text
View/download PDF

8. Author response: Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

Author

Mijnheer, Gerdien, primary, Servaas, Nila Hendrika, primary, Leong, Jing Yao, additional, Boltjes, Arjan, additional, Spierings, Eric, additional, Chen, Phyllis, additional, Lai, Liyun, additional, Petrelli, Alessandra, additional, Vastert, Sebastiaan, additional, de Boer, Rob J, additional, Albani, Salvatore, additional, Pandit, Aridaman, additional, and van Wijk, Femke, additional

Published
2022
Full Text
View/download PDF

9. Compartmentalization and persistence of dominant (regulatory) T cell clones indicates antigen skewing in juvenile idiopathic arthritis

Author

Mijnheer, Gerdien, primary, Servaas, Nila Hendrika, additional, Leong, Jing Yao, additional, Boltjes, Arjan, additional, Spierings, Eric, additional, Chen, Phyllis, additional, Lai, Liyun, additional, Petrelli, Alessandra, additional, Vastert, Sebastiaan, additional, De Boer, Rob J, additional, Albani, Salvatore, additional, Pandit, Aridaman, additional, and van Wijk, Femke, additional

Published
2022
Full Text
View/download PDF

10. Conserved human effector Treg cell transcriptomic and epigenetic signature in arthritic joint inflammation

Author

Infection & Immunity, MMB Research line 1, CTI Van Wijk, Onderzoek Precision medicine, Child Health, Circulatory Health, CTI Van Loosdregt, CTI Pandit, Lab Reumatologie/Klinische Immunologie, CTI Research, CMM Groep Coffer, Immuno/reuma onderzoek 1 (Vastert), Immuno/reuma onderzoek 5 (Vastert2), Immuno/reuma onderzoek 3 (Loosdregt), Mijnheer, Gerdien, Lutter, Lisanne, Mokry, Michal, van der Wal, Marlot, Scholman, Rianne, Fleskens, Veerle, Pandit, Aridaman, Tao, Weiyang, Wekking, Mark, Vervoort, Stephin, Roberts, Ceri, Petrelli, Alessandra, Peeters, Janneke G C, Knijff, Marthe, de Roock, Sytze, Vastert, Sebastiaan, Taams, Leonie S, van Loosdregt, Jorg, van Wijk, Femke, Infection & Immunity, MMB Research line 1, CTI Van Wijk, Onderzoek Precision medicine, Child Health, Circulatory Health, CTI Van Loosdregt, CTI Pandit, Lab Reumatologie/Klinische Immunologie, CTI Research, CMM Groep Coffer, Immuno/reuma onderzoek 1 (Vastert), Immuno/reuma onderzoek 5 (Vastert2), Immuno/reuma onderzoek 3 (Loosdregt), Mijnheer, Gerdien, Lutter, Lisanne, Mokry, Michal, van der Wal, Marlot, Scholman, Rianne, Fleskens, Veerle, Pandit, Aridaman, Tao, Weiyang, Wekking, Mark, Vervoort, Stephin, Roberts, Ceri, Petrelli, Alessandra, Peeters, Janneke G C, Knijff, Marthe, de Roock, Sytze, Vastert, Sebastiaan, Taams, Leonie S, van Loosdregt, Jorg, and van Wijk, Femke

Published
2021

13. Conserved human effector Treg signature is reflected in transcriptomic and epigenetic landscape

Author

Mijnheer, Gerdien, primary, Lutter, Lisanne, additional, Mokry, Michal, additional, van der Wal, Marlot, additional, Fleskens, Veerle, additional, Scholman, Rianne, additional, Pandit, Aridaman, additional, Tao, Weiyang, additional, Wekking, Mark, additional, Vervoort, Stephin, additional, Roberts, Ceri, additional, Petrelli, Alessandra, additional, Peeters, Janneke G.C., additional, Knijff, Marthe, additional, de Roock, Sytze, additional, Vastert, Sebastiaan, additional, Taams, Leonie S., additional, van Loosdregt, Jorg, additional, and van Wijk, Femke, additional

Published
2020
Full Text
View/download PDF

14. T cell regulation and adaptation in chronic inflammation : location matters

Author

Mijnheer, Gerdien, Prakken, Berent, van Wijk, Femke, and University Utrecht

Subjects
FOXP3+ regulatory T cells, Resistance to suppression, Autoimmune inflammation, Effector T cells, T cell adaptation, Juvenile Idiopathic Arthritis, Clonal expansion
Abstract

T cells have an important role in autoimmune diseases as is underscored by genomic research and the abundance of T cells at sites of inflammation. However, in-depth knowledge about the regulation and function of different T cell subsets in local human inflammation is scarce. The recent discovery of tissue resident memory T (Trm) cells and the enormous diversity of regulatory T cell (Treg) subsets identified in different tissues have added a new layer of complexity. Juvenile Idiopathic arthritis (JIA) is an autoimmune disease characterized by chronic inflammation of the joints. As the disease can have a severe impact on the growing skeleton, current treatment strategies are aimed at preventing joint damage by general interference with inflammatory mediators. Treatment also involves intra-articular injections of corticosteroids following aspiration of the inflammatory exudate in the affected joints that allows research of human immune cells that actually take part in the inflammatory process. In this thesis we sought to dissect local immune regulation and T cell programming and adaptation in inflamed joints of Juvenile Idiopathic arthritis (JIA) patients. Analysis of the suppressive function of local SF derived Treg revealed an disruptive effect of the inflammatory environment on T cell regulation. Treg derived from peripheral blood as well as inflamed joints are fully functional. However, effector cells present in SF are unresponsive to Treg suppression due to heightened activation of protein kinase B (PKB)/c-akt that in part is induced by inflammatory mediators, such as TNFα and IL-6. Moreover, we identified an overrepresentation of CD8+PD1+ T cells in SF. From viral infections it is known that CD8+ T cells that express PD-1 are functionally exhausted. However, using gene expression profiling, T cell receptor profiling and functional studies we identified these cells as metabolically active effectors that are clonally expanding and enriched for a Trm profile. Also in target tissues of other human chronic inflammatory diseases increased amounts of this subset were found. These data indicate that CD8+PD1+ T cells represent potential new therapeutic targets. To further study the environment-adapted programming of human Treg, we combined transcriptional and epigenetic profiling of SF Treg. Our data revealed upregulation of core Treg markers as well as effector Treg markers, guided by changes in the (super-)enhancer landscape compared to Treg from blood. The observed profile showed striking overlap with tumor-infiltrating Treg. Thus inflammation seems to induce a specific Treg profile that is fine-tuned by environment-specific adaptations. Additionally, we identified an extensive overlap in T cell immune architecture and specific dominant T(reg) cell clones in anatomically distinct inflammatory sites within a patient. Also, these dominant T cell clones persist over the relapsing-remitting course of disease and can be traced in circulation. Taken together, the studies described in this thesis provide novel insight into T cell regulation and programming in local autoimmune-inflammation. Targeting resistance of effector T cells as wells as aggressive CD8+PD1+ T cells could be of therapeutic interest, whereas circulating clonal T cells similar to those found in the joint can be of clinical interest for monitoring and targeting.

Published
2019

19. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation

Author

Petrelli, Alessandra, Mijnheer, Gerdien, Hoytema Van Konijnenburg, David P., Van Der Wal, Maria M., Giovannone, Barbara, Mocholi, Enric, Vazirpanah, Nadia, Broen, Jasper C., Hijnen, Dirkjan, Oldenburg, Bas, Coffer, Paul J., Vastert, Sebastian J., Prakken, Berent J., Spierings, Eric, Pandit, Aridaman, Mokry, Michal, Van Wijk, Femke, Petrelli, Alessandra, Mijnheer, Gerdien, Hoytema Van Konijnenburg, David P., Van Der Wal, Maria M., Giovannone, Barbara, Mocholi, Enric, Vazirpanah, Nadia, Broen, Jasper C., Hijnen, Dirkjan, Oldenburg, Bas, Coffer, Paul J., Vastert, Sebastian J., Prakken, Berent J., Spierings, Eric, Pandit, Aridaman, Mokry, Michal, and Van Wijk, Femke

Published
2018

20. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation

Author

MMB Research line 1, Infection & Immunity, Lab Dermatologie/Allergologie, Cancer, CMM Groep Coffer, Translationele immunologie, Unit Opleiding Reumat./Klin. Immunologie, MS MDL 1, Onderzoek, CMM Sectie Stem Cells, Regenerative Medicine and Stem Cells, Immuno/reuma onderzoek 5 (Vastert2), Child Health, Immuno/reuma patientenzorg, Circulatory Health, CDL Patiëntenzorg MI, MDL onderzoek 1, Immuno/reuma onderzoek 4, Petrelli, Alessandra, Mijnheer, Gerdien, Hoytema Van Konijnenburg, David P., Van Der Wal, Maria M., Giovannone, Barbara, Mocholi, Enric, Vazirpanah, Nadia, Broen, Jasper C., Hijnen, Dirkjan, Oldenburg, Bas, Coffer, Paul J., Vastert, Sebastian J., Prakken, Berent J., Spierings, Eric, Pandit, Aridaman, Mokry, Michal, Van Wijk, Femke, MMB Research line 1, Infection & Immunity, Lab Dermatologie/Allergologie, Cancer, CMM Groep Coffer, Translationele immunologie, Unit Opleiding Reumat./Klin. Immunologie, MS MDL 1, Onderzoek, CMM Sectie Stem Cells, Regenerative Medicine and Stem Cells, Immuno/reuma onderzoek 5 (Vastert2), Child Health, Immuno/reuma patientenzorg, Circulatory Health, CDL Patiëntenzorg MI, MDL onderzoek 1, Immuno/reuma onderzoek 4, Petrelli, Alessandra, Mijnheer, Gerdien, Hoytema Van Konijnenburg, David P., Van Der Wal, Maria M., Giovannone, Barbara, Mocholi, Enric, Vazirpanah, Nadia, Broen, Jasper C., Hijnen, Dirkjan, Oldenburg, Bas, Coffer, Paul J., Vastert, Sebastian J., Prakken, Berent J., Spierings, Eric, Pandit, Aridaman, Mokry, Michal, and Van Wijk, Femke

Published
2018

21. PD-1+CD8+ T cells are clonally expanding effectors in human chronic inflammation

Author

Petrelli, Alessandra, primary, Mijnheer, Gerdien, additional, van Konijnenburg, David P. Hoytema, additional, van der Wal, Maria M., additional, Giovannone, Barbara, additional, Mocholi, Enric, additional, Vazirpanah, Nadia, additional, Broen, Jasper C., additional, Hijnen, Dirkjan, additional, Oldenburg, Bas, additional, Coffer, Paul J., additional, Vastert, Sebastian J., additional, Prakken, Berent J., additional, Spierings, Eric, additional, Pandit, Aridaman, additional, Mokry, Michal, additional, and van Wijk, Femke, additional

Published
2018
Full Text
View/download PDF

22. Autoimmune disease-associated gene expression is reduced by BET-inhibition

Author

Peeters, Janneke G C, Vervoort, Stephin J, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J, Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Mokry, Michal, van Loosdregt, Jorg, Peeters, Janneke G C, Vervoort, Stephin J, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J, Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Mokry, Michal, and van Loosdregt, Jorg

Published
2016

23. Autoimmune disease-associated gene expression is reduced by BET-inhibition

Author

CMM Groep Coffer, CTI Van Wijk, Infection & Immunity, CTI Van Loosdregt, Child Health, Reumatologie patientenzorg, Zorg en O&O, Regenerative Medicine and Stem Cells, Immuno/reuma onderzoek 1 (Vastert), Circulatory Health, CTI Vastert, Reumatologie onderzoek 2, Divisieleiding O&O, MDL onderzoek 3, Immunologie onderzoek 2, Peeters, Janneke G C, Vervoort, Stephin J, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J, Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Mokry, Michal, van Loosdregt, Jorg, CMM Groep Coffer, CTI Van Wijk, Infection & Immunity, CTI Van Loosdregt, Child Health, Reumatologie patientenzorg, Zorg en O&O, Regenerative Medicine and Stem Cells, Immuno/reuma onderzoek 1 (Vastert), Circulatory Health, CTI Vastert, Reumatologie onderzoek 2, Divisieleiding O&O, MDL onderzoek 3, Immunologie onderzoek 2, Peeters, Janneke G C, Vervoort, Stephin J, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J, Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Mokry, Michal, and van Loosdregt, Jorg

Published
2016

24. Autologous stem cell transplantation aids autoimmune patients by functional renewal and TCR diversification of regulatory T cells

Author

Child Health, Arts-assistenten Kinderen, MMB opleiding Arts microbioloog, CTI Van Wijk, Infection & Immunity, Immuno/reuma onderzoek 2 (Boes), CTI Boes, Reumatologie, Reumatologie patientenzorg, Cluster B, Regenerative Medicine and Stem Cells, Immuno/reuma patientenzorg, Immuno/reuma onderzoek 1 (Vastert), Circulatory Health, CTI Vastert, Reumatologie onderzoek 2, Divisieleiding O&O, CDL Celdiagnostiek, Delemarre, Eveline M., Van Den Broek, Theo, Mijnheer, Gerdien, Meerding, Jenny, Wehrens, Ellen J., Olek, Sven, Boes, Marianne, Van Herwijnen, Martijn J C, Broere, Femke, van Royen, Annet, Wulffraat, Nico W., Prakken, Berent J., Spierings, Eric, Van Wijk, Femke, Child Health, Arts-assistenten Kinderen, MMB opleiding Arts microbioloog, CTI Van Wijk, Infection & Immunity, Immuno/reuma onderzoek 2 (Boes), CTI Boes, Reumatologie, Reumatologie patientenzorg, Cluster B, Regenerative Medicine and Stem Cells, Immuno/reuma patientenzorg, Immuno/reuma onderzoek 1 (Vastert), Circulatory Health, CTI Vastert, Reumatologie onderzoek 2, Divisieleiding O&O, CDL Celdiagnostiek, Delemarre, Eveline M., Van Den Broek, Theo, Mijnheer, Gerdien, Meerding, Jenny, Wehrens, Ellen J., Olek, Sven, Boes, Marianne, Van Herwijnen, Martijn J C, Broere, Femke, van Royen, Annet, Wulffraat, Nico W., Prakken, Berent J., Spierings, Eric, and Van Wijk, Femke

Published
2016

26. Inhibition of Super-Enhancer Activity in Autoinflammatory Site-Derived T Cells Reduces Disease-Associated Gene Expression

Author

Peeters, Janneke G C, Vervoort, Stephin J, Tan, Sander C, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J., Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Creyghton, Menno P, Coffer, Paul J, Mokry, Michal, van Loosdregt, Jorg, Peeters, Janneke G C, Vervoort, Stephin J, Tan, Sander C, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J., Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Creyghton, Menno P, Coffer, Paul J, Mokry, Michal, and van Loosdregt, Jorg

Published
2015

27. Inhibition of Super-Enhancer Activity in Autoinflammatory Site-Derived T Cells Reduces Disease-Associated Gene Expression

Author

CMM Groep Coffer, CTI Van Wijk, Infection & Immunity, CTI Van Loosdregt, Child Health, Immuno/reuma onderzoek 1 (Vastert), Reumatologie patientenzorg, Zorg en O&O, Regenerative Medicine and Stem Cells, Circulatory Health, CTI Vastert, Reumatologie onderzoek 2, Hubrecht Institute with UMC, Immunologie onderzoek 2, Cancer, MDL onderzoek 3, Peeters, Janneke G C, Vervoort, Stephin J, Tan, Sander C, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J., Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Creyghton, Menno P, Coffer, Paul J, Mokry, Michal, van Loosdregt, Jorg, CMM Groep Coffer, CTI Van Wijk, Infection & Immunity, CTI Van Loosdregt, Child Health, Immuno/reuma onderzoek 1 (Vastert), Reumatologie patientenzorg, Zorg en O&O, Regenerative Medicine and Stem Cells, Circulatory Health, CTI Vastert, Reumatologie onderzoek 2, Hubrecht Institute with UMC, Immunologie onderzoek 2, Cancer, MDL onderzoek 3, Peeters, Janneke G C, Vervoort, Stephin J, Tan, Sander C, Mijnheer, Gerdien, de Roock, Sytze, Vastert, Sebastiaan J., Nieuwenhuis, Edward E S, van Wijk, Femke, Prakken, Berent J, Creyghton, Menno P, Coffer, Paul J, Mokry, Michal, and van Loosdregt, Jorg

Published
2015

28. Inhibition of Super-Enhancer Activity in Autoinflammatory Site-Derived T Cells Reduces Disease-Associated Gene Expression

Author

Peeters, Janneke G.C., primary, Vervoort, Stephin J., additional, Tan, Sander C., additional, Mijnheer, Gerdien, additional, de Roock, Sytze, additional, Vastert, Sebastiaan J., additional, Nieuwenhuis, Edward E.S., additional, van Wijk, Femke, additional, Prakken, Berent J., additional, Creyghton, Menno P., additional, Coffer, Paul J., additional, Mokry, Michal, additional, and van Loosdregt, Jorg, additional

Published
2015
Full Text
View/download PDF

30. Staphylococcus aureusStaphopain A inhibits CXCR2-dependent neutrophil activation and chemotaxis

Author

Laarman, Alexander J, primary, Mijnheer, Gerdien, additional, Mootz, Joe M, additional, van Rooijen, Willemien J M, additional, Ruyken, Maartje, additional, Malone, Cheryl L, additional, Heezius, Erik C, additional, Ward, Richard, additional, Milligan, Graeme, additional, van Strijp, Jos A G, additional, de Haas, Carla J C, additional, Horswill, Alexander R, additional, van Kessel, Kok P M, additional, and Rooijakkers, Suzan H M, additional

Published
2012
Full Text
View/download PDF

31. Functional human regulatory T cells fail to control autoimmune inflammation due to PKB/c-akt hyperactivation in effector cells

Author

Wehrens, Ellen J., primary, Mijnheer, Gerdien, additional, Duurland, Chantal L., additional, Klein, Mark, additional, Meerding, Jenny, additional, van Loosdregt, Jorg, additional, de Jager, Wilco, additional, Sawitzki, Birgit, additional, Coffer, Paul J., additional, Vastert, Bas, additional, Prakken, Berent J., additional, and van Wijk, Femke, additional

Published
2011
Full Text
View/download PDF

32. Autoimmune disease-associated gene expression is reduced by BET-inhibition.

Author

Peeters JG, Vervoort SJ, Mijnheer G, de Roock S, Vastert SJ, Nieuwenhuis EE, van Wijk F, Prakken BJ, Mokry M, and van Loosdregt J

Abstract

For many autoimmune diseases, the underlying mechanism is still unknown. In order to get more insight into the etiology of autoimmune diseases, we recently published a study were we performed epigenetic profiling and RNA sequencing on CD4(+)CD45RO(+) T cells derived from the site of inflammation of Juvenile Idiopathic Arthritis (JIA) patients and compared this with healthy controls [1]. In this "Data in Brief", we focus on the analysis of our RNA sequencing data reported in this study, of which the raw and processed files can be found in GEO under GSE71595. We provide a detailed description of the downstream analysis, quality controls, and different analysis methods or techniques that validate the results obtained with RNA-sequencing.

Published
2015
Full Text
View/download PDF

33. Anti-tumor necrosis factor α targets protein kinase B/c-Akt-induced resistance of effector cells to suppression in juvenile idiopathic arthritis.

Author

Wehrens EJ, Vastert SJ, Mijnheer G, Meerding J, Klein M, Wulffraat NM, Prakken BJ, and van Wijk F

Subjects
Adolescent, Antirheumatic Agents therapeutic use, Arthritis, Juvenile drug therapy, Cell Proliferation drug effects, Child, Etanercept, Humans, Immunoglobulin G therapeutic use, Interleukin-6 antagonists & inhibitors, Leukocytes, Mononuclear drug effects, Leukocytes, Mononuclear metabolism, Receptors, Tumor Necrosis Factor therapeutic use, Signal Transduction physiology, Synovial Fluid drug effects, Synovial Fluid metabolism, T-Lymphocytes, Regulatory drug effects, T-Lymphocytes, Regulatory metabolism, Antirheumatic Agents pharmacology, Arthritis, Juvenile metabolism, Immunoglobulin G pharmacology, Protein Kinase C metabolism, Proto-Oncogene Proteins c-akt metabolism, Signal Transduction drug effects, Tumor Necrosis Factor-alpha antagonists & inhibitors
Abstract

Objective: To determine whether therapeutic strategies that block interleukin-6 (IL-6) or tumor necrosis factor α (TNFα) can improve the responsiveness of Teff cells to suppression in patients with juvenile idiopathic arthritis (JIA)., Methods: Synovial fluid mononuclear cells (SFMCs) from the inflamed joints of patients with JIA were cultured in the presence of etanercept or anti-IL-6 in vitro, and protein kinase B (PKB)/c-Akt activation and responsiveness to suppression were measured. In addition, the in vivo effects of TNFα blockade were investigated using peripheral blood mononuclear cells obtained from patients before and after the start of etanercept therapy., Results: In vitro treatment of SFMCs with anti-IL-6 led to improved Treg cell-mediated suppression of cell proliferation in some but not all patients. Blocking TNFα with etanercept, however, clearly enhanced suppression, especially that of CD8+ T cells. In the presence of etanercept, PKB/c-Akt activation of Teff cells was reduced, and cells became more susceptible to transforming growth factor β-mediated suppression, indicating that anti-TNFα directly targets resistant Teff cells., Conclusion: This study is the first to show that anti-TNFα targets the resistance of Teff cells to suppression, resulting in improved regulation of inflammatory effector cells., (Copyright © 2013 by the American College of Rheumatology.)

Published
2013
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results