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Microbial fossils preserved by early diagenetic chert provide a window into the Proterozoic biosphere, but seawater chemistry, microbial processes, and the interactions between microbes and the environment that contributed to this preservation are not well constrained. Here, we use fossilization experiments to explore the processes that preserve marine cyanobacterial biofilms by the precipitation of amorphous silica in a seawater medium that is analogous to Proterozoic seawater. These experiments demonstrate that the exceptional silicification of benthic marine cyanobacteria analogous to the oldest diagnostic cyanobacterial fossils requires interactions among extracellular polymeric substances (EPS), photosynthetically induced pH changes, magnesium cations (Mg2+ ), and >70 ppm silica.

Silicification was a major mode of fossilization in Proterozoic peritidal environments, but marine silica concentrations and the chemical and biological mechanisms that drove microbial silicification and formation of early diagenetic chert in these environments remain poorly constrained. Here, we use taphonomy experiments to demonstrate that photosynthetically active cyanobacteria that are morphologically analogous to the oldest cyanobacterial fossil, Eoentophysalis, mediate the formation of magnesium-rich amorphous silica in seawater that is undersaturated with respect to silica. These results show that microbes in Proterozoic tidal environments may have mediated their own silicification at lower silica concentrations than previously assumed.

Theory and observation for the search for life on exoplanets via atmospheric "biosignature gases" is accelerating, motivated by the capabilities of the next generation of space- and ground-based telescopes. The most observationally accessible rocky planet atmospheres are those dominated by molecular hydrogen gas, because the low density of H$_2$-gas leads to an expansive atmosphere. The capability of life to withstand such exotic environments, however, has not been tested in this context. We demonstrate that single-celled microorganisms ($\textit{E. coli}$ and yeast) that normally do not inhabit H$_2$-dominated environments can survive and grow in a 100% H$_2$ atmosphere. We also describe the astonishing diversity of dozens of different gases produced by $\textit{E. coli}$, including many already proposed as potential biosignature gases (e.g., nitrous oxide, ammonia, methanethiol, dimethylsulfide, carbonyl sulfide, and isoprene). This work demonstrates the utility of lab experiments to better identify which kinds of alien environments can host some form of possibly detectable life., Nature Astronomy https://doi.org/10.1038/s41550-020-1069-4. V2 has a typo correction

Hot super Earths are exoplanets with short orbital periods ($$ 0.4) in the Kepler band (420-900 nm). We are motivated to determine whether reflection from molten lava and quenched glasses (a product of rapidly cooled lava) on the surfaces of hot super Earths contributes to the observationally inferred high geometric albedos. We experimentally measure reflection from rough and smooth textured quenched glasses of both basalt and feldspar melts. For lava reflectance values, we use specular reflectance values of molten silicates from non-crystalline solids literature. Integrating the empirical glass reflectance function and non-crystalline solids reflectance values over the dayside surface of the exoplanet at secondary eclipse yields an upper limit for the albedo of a lava-quenched glass planet surface of $\sim $0.1. We conclude that lava planets with solid (quenched glass) or liquid (lava) surfaces have low albedos. The high albedos of some hot super Earths are most likely explained by atmospheres with reflective clouds (or, for a narrow range of parameter space, possibly Ca/Al oxide melt surfaces). Lava planet candidates in TESS data can be identified for follow-up observations and future characterization., Comment: 18 pages, 14 figures, 4 tables, published in ApJ

Nanospacecraft could enable detailed characterization of many asteroids in a small timeframe by launching multiple spacecraft simultaneously to visit a large set of targets. To be able to characterize as large of a set of asteroids as possible, however, visits to individual asteroids would be limited to flybys, which would have to be autonomous. As an additional challenge, due to the limitations of nanospacecraft and a massively parallel architecture, Earth-based localization and communication infrastructure, such as the Deep Space Network, cannot be relied upon. We have developed an optical instrument prototype and an image simulation system for autonomous asteroid flybys and 3D multispectral mapping using nanospacecraft. The final system is targeted to fit into a single CubeSat unit (a 10 cm cube) and to have the mass of less than 1 kg. The images are used for structure from motion algorithms to determine the quality of 3D reconstruction to be expected from this mission. We are presenting the instrument design, the simulation system, and the simultaneous localization and mapping system developed for nanospacecraft asteroid flybys.

Siliciclastic sediments of the Ediacaran Period contain exceptionally preserved fossils of macroscopic organisms, including three-dimensional casts and molds commonly found in sandstones and siltstones and some two-dimensional compressions reported in shales. The sporadic and variable associations of these exceptionally preserved macroscopic fossils with pyrite, clay minerals, and microbial fossils and textures complicate our understanding of fossilization processes. This hinders inferences about the evolutionary histories, tissue types, original morphologies, and lifestyles of the enigmatic Ediacara biota. Here, we investigate the delayed decay of scallop muscles buried in quartz sand or kaolinite for 45 days. This process occurs in the presence of microbial activity in mixed redox environments, but in the absence of thick, sealing microbial mats. Microbial processes that mediate organic decay and release the highest concentrations of silica and Fe(II) into the pore fluids are associated with the most extensive tissue decay. Delayed decay and the preservation of thick muscles in sand are associated with less intense microbial iron reduction and the precipitation of iron oxides and iron sulfides that contain Fe(II) or Fe(III). In contrast, muscles buried in kaolinite are coated only by, NASA Astrobiology Institute (Grant NNA13AA90A), Simons Foundation (Grants 327126 and 344707), American Chemical Society (Award 54498-ND8)

During the course of a space mission unexpected events can occur regardless of rigorous testing. In order to ensure the ability of a spacecraft to recover and adapt to new situations, it may be necessary to update the firmware for resolving the software issues, work around hardware problems, or introduce new features. The importance of remote firmware updates as well as a method to calculate an indicative value of flexibility in space missions is summarized by R. Nilchiani [1].

Oxygenic photosynthesis supplies organic carbon to the modern biosphere, but it is uncertain when this metabolism originated. It has previously been proposed1,2 that photosynthetic reaction centres capable of splitting water arose by about 3billion years ago on the basis of the inferred presence of manganese oxides in Archaean sedimentary rocks. However, this assumes that manganese oxides can be produced only in the presence of molecular oxygen3, reactive oxygen species4,5 or by high-potential photosynthetic reaction centres6,7. Here we show that communities of anoxygenic photosynthetic microorganisms biomineralize manganese oxides in the absence of molecular oxygen and high-potential photosynthetic reaction centres. Microbial oxidation of Mn(II) under strictly anaerobic conditions during the Archaean eon would have produced geochemical signals identical to those used to date the evolution of oxygenic photosynthesis before the Great Oxidation Event1,2. This light-dependent process may also produce manganese oxides in the photic zones of modern anoxic water bodies and sediments.

We propose a distributed close-range survey of hundreds of asteroids representing many asteroid families, spectral types and sizes. This can be implemented by a fleet of nanospacecraft (e.g., 4–5-unit CubeSats) equipped with miniature imaging and spectral instruments (from near ultraviolet to near infrared). To enable the necessary large delta-v, each spacecraft carries a single electric sail tether which taps the momentum from the solar wind. Data are stored in a flash memory during the mission and downlinked at an Earth flyby. This keeps deep-space network telemetry costs down, despite the large number of individual spacecraft. To navigate without the use of the deep-space network, optical navigation is required to track stars, planets and asteroids. The proposed mission architecture is scalable both scientifically and financially. A fleet of 50 spacecraft will be able to obtain images and spectral data from 200 to 300 near-Earth and main belt asteroids. It allows study of those asteroid families and spectroscopic types for which currently no close-range observations are available. This paper presents science objectives, overall science traceability matrix, example targets and technical challenges associated with the mission. We open to discuss preliminary requirements, mission and spacecraft designs.

ESTCube-1 is a student satellite project lead by the University of Tartu, Estonia, and supported by the European Space Agency (ESA) via Plan for European Cooperating States (PECS). Development of ESTCube-1 has been a collaborative effort with many international partners. The satellite is shown on Figure 1 [1].

Difference fluorescence line-narrowing spectra are studied for evaluating the temperature dependence of the linear electron–phonon and vibronic coupling strengths in the two weakly coupled low-temperature glassy systems: chlorin-doped 1-propanol and the native Fenna–Matthews–Olson (FMO) light-harvesting protein complex folded with bacteriochlophyll a chromophores. Roughly three-fold increases of the electron–phonon coupling strength and constant vibronic couplings were observed in both systems in the available temperature range from 4.5 to 70 K. However, while the chlorin system is amenable to simple modeling of impurity spectra, the FMO shows significant deviations, consistent with the complex (exciton) nature of its lowest-energy singlet electronic transition.

The scientific mission of ESTCube-1, launched in May 2013, is to measure the electric solar wind sail (E-sail) force in orbit. The experiment is planned to push forward the development of the E-sail, a propulsion method recently invented at the Finnish Meteorological Institute. The E-sail is based on extracting momentum from the solar wind plasma flow by using long thin electrically charged tethers. ESTCube-1 is equipped with one such tether, together with hardware capable of deploying and charging it. At the orbital altitude of ESTCube-1 (660–680 km) there is no solar wind present. Instead, ESTCube-1 shall observe the interaction between the charged tether and the ionospheric plasma. The ESTCube-1 payload uses a 10-m, partly two-filament E-sail tether and a motorized reel on which it is stored. The tether shall be deployed from a spinning satellite with the help of centrifugal force. An additional mass is added at the tip of the tether to assist with the deployment. During the E-sail experiment the tether shall be charged to 500 V potential. Both positive and negative voltages shall be experimented with. The voltage is provided by a dedicated high-voltage source and delivered to the tether through a slip ring contact. When the negative voltage is applied to the tether, the satellite body is expected to attract the electron flow capable of compensating for the ion flow, which runs to the tether from the surrounding plasma. With the positive voltage applied, onboard cold cathode electron guns are used to remove excess electrons to maintain the positive voltage of the tether. In this paper we present the design and structure of the tether payload of ESTCube-1.

In photosynthesis, collective pigment excitations – excitons – facilitate chemical reactions for sustainable biological function. Here, the effect of hydrostatic pressure – an important thermodynamic stress factor – on optical spectral properties of excitons in the cyclic light-harvesting 2 pigment-protein complex from photosynthetic bacterium Rhodoblastus acidophilus was first studied. The high pressure-induced modifications of absorption, fluorescence and polarized fluorescence excitation spectra were theoretically analyzed in terms of the disordered molecular exciton model. We uniquely show that the observed shift of the spectra under pressure is largely governed by the pressure-induced rise of two factors: the exciton displacement energy and the exciton coupling energy, which increases the spread of the exciton state manifold. A significant increase of static energy disorder revealed by model calculations suggests that high-pressure compressing of the complex is actually accompanied by altered spatial orientations and conformations of the protein-embedded pigment molecules.

Live cells and regular crystals seem fundamentally incompatible. Still, effects characteristic to ideal crystals, such as coherent sharing of excitation, have been recently used in many studies to explain the behavior of several photosynthetic complexes, especially the inner workings of the light-harvesting apparatus of the oldest known photosynthetic organisms, the purple bacteria. To this date, there has been no concrete evidence that the same effects are instrumental in real living cells, leaving a possibility that this is an artifact of unnatural study conditions, not a real effect relevant to the biological operation of bacteria. Hereby, we demonstrate survival of collective coherent excitations (excitons) in intact cells of photosynthetic purple bacteria. This is done by using excitation anisotropy spectroscopy for tracking the temperature-dependent evolution of exciton bands in light-harvesting systems of increasing structural complexity. The temperature was gradually raised from 4.5 K to ambient temperature, and the complexity of the systems ranged from detergent-isolated complexes to complete bacterial cells. The results provide conclusive evidence that excitons are indeed one of the key elements contributing to the energetic and dynamic properties of photosynthetic organisms.

By applying an advanced hole-burning fluorescence line-narrowing technique, an unexpectedly strong (several-fold) increase of the electron–phonon coupling strength on excitation wavelength through the inhomogenously broadened absorption origin band was first observed for low-temperature organic glass samples doped with biologically relevant chlorin and chlorophyll a molecules. The dependence, which suggests a direct correlation between solvent shift and the electron–phonon coupling, demonstrates great sensitivity of the zero-phonon transitions localized on single chromophores as optical probes of the structure and dynamics of local subnanoscopic environments.

Bacterial light-harvesting pigment-protein complexes are very efficient at converting photons into excitons and transferring them to reaction centers, where the energy is stored in a chemical form. Optical properties of the complexes are known to change significantly in time and also vary from one complex to another; therefore, a detailed understanding of the variations on the level of single complexes and how they accumulate into effects that can be seen on the macroscopic scale is required. While experimental and theoretical methods exist to study the spectral properties of light-harvesting complexes on both individual complex and bulk ensemble levels, they have been developed largely independently of each other. To fill this gap, we simultaneously analyze experimental low-temperature single-complex and bulk ensemble optical spectra of the light-harvesting complex-2 (LH2) chromoproteins from the photosynthetic bacterium Rhodopseudomonas acidophila in order to find a unique theoretical model consistent with both experimental situations. The model, which satisfies most of the observations, combines strong exciton-phonon coupling with significant disorder, characteristic of the proteins. We establish a detailed disorder model that, in addition to containing a C_{2}-symmetrical modulation of the site energies, distinguishes between static intercomplex and slow conformational intracomplex disorders. The model evaluations also verify that, despite best efforts, the single-LH2-complex measurements performed so far may be biased toward complexes with higher Huang-Rhys factors.

In this work, the design of the electrical power system developed for ESTCube-1 – the first Estonian satellite with the first test mission of electric solar wind sail – is presented. The mission is highly energy-intensive, and, since its complexity might lead to many possible failure cases, it requires a very efficient and fault-tolerant solution. The system has been developed from ground up, using only commercial-off-the-shelf components and student workforce. It includes several innovations, which help the system to be more reliable, efficient and controllable than earlier nanosatellite power system designs.

The nature of electronic excitations created by photon absorption in the cyclic B850 aggregates of 18 bacteriochlorophyll molecules of LH2 antenna complexes of photosynthetic bacteria is studied over a broad temperature range using absorption, fluorescence, and fluorescence anisotropy spectra. The latter technique has been proved to be suitable for revealing the hidden structure of excitons in inhomogeneously broadened spectra of cyclic aggregates. A theoretical model that accounts for differences of absorbing excitons in undeformed and emitting exciton polarons in deformed antenna lattices is also developed. Only a slight decrease of the exciton bandwidth and exciton coupling energy with temperature is observed. Survival of excitons in the whole temperature span from cryogenic to nearly ambient temperatures strongly suggests that collective, coherent electronic excitations might play a role in the functional light-harvesting process taking place at physiological temperatures.

We have observed that an assembly of the bacteriochloropyll a molecules into B850 and B875 groups of cyclic bacterial light-harvesting complexes LH2 and LH1, respectively, results an almost total loss of the intra-molecular vibronic structure in the fluorescence spectrum, and simultaneously, an essential enhancement of its phonon sideband due to electron-phonon coupling. While the suppression of the vibronic coupling in delocalized (excitonic) molecular systems is predictable, as also confirmed by our model calculations, a boost of the electron-phonon coupling is rather unexpected. The latter phenomenon is explained by exciton self-trapping, promoted by mixing the molecular exciton states with charge transfer states between the adjacent chromophores in the tightly packed B850 and B875 arrangements. Similar, although less dramatic trends were noted for the light-harvesting complexes containing chlorophyll pigments.

Received 21 August 2013, revised 21 April 2014, accepted 22 April 2014, available online 23 May 2014 Abstract. This work describes the final design and implementation of the electrical power system for ESTCube-1, a 1-unit CubeSat tasked with testing the electrostatic tether concept and associated technologies for the electric solar wind sail in polar low Earth orbit. The mission required an efficient and reliable power system to be designed that could efficiently handle highly variable power requirements and protect the satellite from damage caused by malfunctions in its individual subsystems, while using only commercial-off-the-shelf components. The system was developed from scratch and includes a novel redundant stand- alone nearly 90% efficient maximum power point tracking system, based on a commercially available integrated circuit, a lithium- ion battery based fault-tolerant power storage solution, a highly controllable and monitorable power distribution system, capable of sustaining loads of up to 10 W, and an AVR microcontroller based control solution, heavily utilizing non-volatile ferroelectric random access memories. The electrical power system was finalized in January 2013 and was launched into orbit on 7th of May, 2013. In this paper, we describe the requirements for the subsystem, the design of the subsystem, pre-flight testing, and flight qualification.

We present a portable optical cataract assessment technology which measures with a circular photodetector the fraction of light scattered backwards by the human eye lens when illuminated by a laser diode. As our signal arises directly from the fundamental pathology-increased scattering in the lens-it directly assesses cataract extent and progression. Initial clinical results in undilated human eyes show device reading correlations in agreement with clinical examination and Scheimpflug photography.

This paper presents two technology experiments – the plasma brake for deorbiting and the electric solar wind sail for interplanetary propulsion – on board the ESTCube-2 and FORESAIL-1 satellites. Since both technologies employ the Coulomb interaction between a charged tether and a plasma flow, they are commonly referred to as Coulomb drag propulsion. The plasma brake operates in the ionosphere, where a negatively charged tether deorbits a satellite. The electric sail operates in the solar wind, where a positively charged tether propels a spacecraft, while an electron emitter removes trapped electrons. Both satellites will be launched in low Earth orbit carrying nearly identical Coulomb drag propulsion experiments, with the main difference being that ESTCube-2 has an electron emitter and it can operate in the positive mode. While solar-wind sailing is not possible in low Earth orbit, ESTCube-2 will space-qualify the components necessary for future electric sail experiments in its authentic environment. The plasma brake can be used on a range of satellite mass classes and orbits. On nanosatellites, the plasma brake is an enabler of deorbiting – a 300-m-long tether fits within half a cubesat unit, and, when charged with - 1 kV, can deorbit a 4.5-kg satellite from between a 700- and 500-km altitude in approximately 9–13 months. This paper provides the design and detailed analysis of low-Earth-orbit experiments, as well as the overall mission design of ESTCube-2 and FORESAIL-1.