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ABSTRACT The objective of this study was to estimate the genetic parameters and predict selection of genetic gains and genetic diversity of 12 Andropogon lateralis ecotypes collected in the State of Rio Grande do Sul, Brazil. To estimate genetic parameters and predict selection gains, the REML/BLUP technique was applied. Genetic diversity among the ecotypes was evaluated by two clustering methods (optimization and hierarchical) and principal component (PC) analysis, the latter method also used to discard variables. The genetic parameters studied showed high potential for selection of important agronomic forage traits for livestock production. Results showed that the 12 A. lateralis Nees ecotypes exhibited high genetic variability for the studied forage characters and indicated parental prosperity for crosses within the genetic breeding program. Principal component analysis showed that number of total vegetative tillers, leaf:stem ratio, number of reproductive tillers, and leaf dry matter yield accounted for 80.6% of the observed variation in PC1. These variables are important characteristics for quantifying the dry matter production and nutritional value of forage plants, and they can help to discriminate amongst ecotypes. Ecotypes sourced from the Pelotas, Piratini, Passo Fundo, Bagé, and Montenegro regions showed superior forage production when evaluated by the BLUP methodology. Therefore, this group was identified as the most suitable for selection and crossing purposes. Tocher’s cluster analysis grouped the ecotypes into five divergent groups. Principal component and UPGMA hierarchical methods were also efficient at separating the ecotypes.

Abstract The Paspalum genus has potential for further genetic improvement because of its adaptability to different ecosystems and production of high yields for grazing livestock. We estimate the genetic parameters of 195 intraspecific P. notatum hybrids using Restricted Maximum Likelihood (REML), followed by selection based on Best Linear Unbiased Prediction (BLUP) through multivariate analysis. The intraspecific hybrids studied showed considerable genetic variability in the evaluated forage traits, displaying their potential for progression in subsequent stages of the genetic improvement program. Notably, plant height emerged as an important trait for indirect selection to enhance forage production. The use of the REML/BLUP procedure proves to be a robust tool for data analysis, particularly for perennial species. Furthermore, multivariate analysis based on BLUPs should be used in the selection process within breeding programs. Based on the BLUP values, hybrids D3, D16, C17, C2 and B17 were identified as superior for forage production, and they hold promise for future breeding programs for future breeding initiatives aimed at direct selection to improve yield.

ABSTRACT The objective of this study was to verify the repeatability of the expression of forage characters in intraspecific hybrids of Paspalum notatum Flügge to aid early selection. Across five harvests, plant height, tiller population density, leaf dry matter, stem dry matter, inflorescence dry matter, total dry matter, and growth habit were quantified for five parents, 189 hybrids, and a commercially available cultivar as a control (n = 195). Analysis of variance, principal components analysis, and structural analysis methods were used to determine the repeatability coefficients. The repeatability coefficients ( ρ ^) for all evaluated characteristics generated by the different methods were between 0.05 (ANOVA II) and 0.95 (PCACov). For most of the characteristics studied, repeatability coefficients and determination coefficients were considered high. The repeatability coefficients estimates obtained for the eight characteristics evaluated with the ANOVA I and II methods were almost always lower than those obtained by PCA and structural analysis methods. Based on the covariance matrix, the principal component method generated higher estimates than those produced by ANOVA or structural analysis. Assuming a minimum 80% reliability to verify the relative superiority of the hybrids across all assessed traits, the five harvests proved adequate for selecting the optimal plant materials to advance to the next phase of the breeding program. However, reliable early selection for leaf dry matter, leaf:stem ratio, and total dry matter required a minimum of two harvests. The genetic parameters (h2 and CVg) showed a favorable scenario for direct selection to increase forage production.

Bahiagrass (Paspalum notatum Flügge) is an important forage in South America and the United States because of its high palatability, resistance to trampling and grazing, and tolerance to cold, but it exhibits low seed yield and poor seed quality. Previous studies reported improvements in forage production and nutritive value in hybrids and ecotypes; however, information about seed yield and quality in forage bahiagrass is limited. This study aimed to characterize the seed yield and quality of nine P. notatum intraspecific hybrids and three controls: P. notatum ecotypes V4 and Bagual and cultivar Pensacola. Inflorescence density, 1,000-seed weight, seed yield and germination rate decreased in year 2 influenced by weather conditions and ergot. Seed yield ranged from 139 (Pensacola) to 1,158 (Bagual) kg ha-1 among all entries, where Bagual, C18, and V4 produced more than 974 kg ha-1, which was approximately seven times more than Pensacola. Bagual, C18, V4, 336, C15, 225, and D3 showed germination rates greater than 83%. In conclusion, Bagual had the highest seed yield and germination rate, whereas hybrids C18 and 336 showed high seed yield, 1,000-seed weight, and germination rate in both years. Bagual, C18, and 336 should be used in future breeding programs to improve seed production traits. Our study revealed that selection for seed production traits can result in improvements in seed yield and quality in bahiagrass.

ABSTRACT The objective of this work was to describe morphology and grouping of Paspalum notatum accessions, based on multicategorical data which discards the redundant variables for quantification of genetic diversity. We also tested the hypothesis that geographical distance was correlated with morphological divergence. In our study, multivariate analyzes successfully demonstrated the geographic and morphological variability of the P. notatum accessions characterized. Many of these evaluated accessions can be included in future genetic improvement programs. Based on two methodologies for discarding variables, it was possible to identify the potentially important morphological characteristics from genetic diversity studies and characterize new accessions aimed at improving forage and seed production. The methodologies used to discard variables are biometric tools that can be used successfully in future plant breeding programs, especially when a large number of traits and accessions are being evaluated. Although significant, geographic distance had a low association with morphological traits. This indicated the need to use other characteristics, such as forage and seed yield, in addition to molecular analysis. Our analyzes showed genetic variability in P. notatum for all the characteristics studied.

Selection of improved genotypes is important for pasture-based feeding systems in subtropical regions. Our goal was to identify hybrids of Paspalum with enhanced forage yield and cold tolerance across 2 sites [Bagé and Eldorado do Sul (ES)], in Rio Grande do Sul, Brazil. We evaluated 19 P. plicatulum × P. guenoarum hybrids, P. plicatulum genotype 4PT, P. guenoarum cultivars Azulão and Baio and, as Control, Megathyrsus maximus cv. Aruana. At both sites, the experimental design was a completely randomized block with 4 replications. Total dry mass (total-DM), leaf-DM and cold tolerance (ColdT) were recorded. At Bagé, hybrid 102069 produced higher total-DM and leaf-DM than the progenitors and cv. Aruana, while at ES, hybrids 102069 and 10308 produced higher total-DM than 4PT, Azulão and Aruana; hybrid 102069 had higher leaf-DM. At Bagé, 16 hybrids displayed ColdT similar to their progenitors and higher than Aruana, while at ES, 12 hybrids showed ColdT similar to Azulão and Baio and higher than 4PT and Aruana. This study demonstrated that hybrids of Paspalum with superior forage yield to their progenitors and Aruana, and hybrids with higher ColdT than 4PT and Aruana are in existence. The hybridization technique shows potential for producing alternative genotypes with higher forage yield and ColdT for sowing in subtropical regions.

In Southern Brazil, much of the livestock activity is developed in native grasslands. Studies about forage traits, frost tolerance and nutritional value of native forage species there has been increasing with better results of productive efficiency (animal gain). The objective of this work was to evaluate forage yield of forty-five intraspecific hybrids of segregating progenies of bahiagrass (Paspalum notatum Flügge) from agronomic analyses under greenhouse conditions and determine the reproduction mode of selected hybrids. The hybrids had great variability in all agronomic characteristics measured. The plants with the highest total dry mass production were 17PN10P3 and 17PN29P1 (sexual reproduction), 17PN10P5, 17PN16P3 and 17PN28P4 (apomictic reproduction). The high correlation of the total dry mass with tillers number and with the root dry mass demonstrate that the hybrids can be used as forage and also to reduce the effects of soil erosion in degraded areas. The evaluations of agronomic characteristics and the determination of the mode of reproduction of the selected hybrids allowed the identification of promising genetic materials for forage yield and to soil cover, selecting them for additional stages in the breeding program.

ABSTRACT The objective of this research was to identify the existence of root exudation of organic acid in Lotus corniculatus germplasms subjected to toxic aluminum (Al) levels and investigate the effect of this mechanism on the Al content in the root tissue and in morphological parameters of plant development. Two experiments were performed in nutrient solution to evaluate the Al accumulation and exudation of organic acids, using cultivars INIA Draco and São Gabriel and genotypes UFRGS and UF-T2. The plants were cultivated in Al-free solution, which was applied on the 45th day in half of the pots of each genotype. Root exudation was highly correlated with the reduced accumulation of Al in the root tissue (r2 = 0.75 at 72 h). Genotype UF-T2, selected for Al tolerance, extruded 80% more oxalic acid in the presence of Al compared with the other germplasms, indicating that this mechanism is involved with Al tolerance in L. corniculatus. This experiment showed strong evidence that L. corniculatus exhibits Type I tolerance, in which anionic channels are rapidly activated by Al exposure. Exudation of oxalic acid is likely a crucial mechanism that allows the maintenance of L. corniculatus growth when exposed to toxic Al conditions, and this characteristic should be used to identify tolerant genotypes in the future.

ABSTRACT The objective of this study was to evaluate 94 Paspalum notatum genotypes over two growing seasons to estimate genetic dissimilarity through agronomic traits and the distance between genotypes. This information is used to create an ideotype from the best averages obtained for the set of characteristics evaluated. Seven apomitic, three sexual, and 81 hybrid genotypes were compared with native genotypes “André da Rocha”, “Bagual”, and cultivar “Pensacola” as controls. There is genetic variability in P. notatum for the studied characteristics, and distinct genotypes with superior characteristics can be used in new combinations between apomictic and sexual plants to obtain hybrids. The characters with the greatest relative contribution to the dissimilarity between the genotypes were tiller density, stem dry mass, and leaf dry mass yield. Thus, these characteristics are suitable criteria to infer genetic distance studies in P. notatum . The selection index based on the ideotype is an auxiliary tool in the breeding process. The ideotype must be based on characteristics of interest according to the objective of the breeding program, as well as on the breeder’s prior knowledge in relation to culture.

ABSTRACT: Andropogon lateralis Nees is a native grass of southern Brazil and is one of the most frequent specie found in native grasslands. The species is widely distributed and has a high degree of phenotypic plasticity, which makes it highly adaptable to different edaphoclimatic conditions and management. This study aimed to evaluate the behavior of twelve ecotypes of A. lateralis, collected in different regions of the state of Rio Grande do Sul and cut to three different heights and subjected to two different defoliation frequencies. From September to February, the ecotypes were evaluated for total dry matter, leaf and stem yields. In addition, total, vegetative and reproductive tillers and plant height were measured. These characteristics are important for the selection of superior genotypes in terms of genetic variability and forage production. Total dry matter and leaf dry matter are characteristics with agronomic importance and they showed the highest correlation (r = 0.77), enabling an indirect selection for one of these characteristics. The natural selection of plants resulted in distinct structural, morphological and productive characteristics with heterogeneity that allows the selection and grouping according to the characteristics, ecotypes with superior agronomic characteristics can be included in breeding programs.

ABSTRACT The objective of this work was to determine Paspalum notatum genotypes appropriate for the establishment of mixtures with temperate legumes and compare the productivity of mixtures with mineral-fertilized systems along two years. Four hybrids of P. notatum, ecotype Bagual and cv. Pensacola, were either subjected to mixtures with white clover (Trifolium repens) and birdsfoot trefoil (Lotus corniculatus) or fertilized with 0, 60, 120, 240, and 480 kg N ha−1 year−1. P. notatum leaf blades and stems and herbage accumulation denoted a management × year interaction. Herbage accumulation varied between the mixtures and fertilized systems in first year, while in the second year, herbage accumulation of mixed systems employing the B26, B43, C22, C9, and Bagual genotypes were similar to that of systems fertilized with 120 and 240 kg N ha−1. Hybrids B26 and C9 and ecotype Bagual, once made available to producers, could be indicated for mixtures with temperate legumes because they are superior to cv. Pensacola. Herbage accumulation in mixtures involving white clover plus birdsfoot trefoil and P. notatum genotypes is similar to that of mineral nitrogen-fertilized systems with 240 kg N ha−1, emphasizing the viability of mixed between these species.

The native grasslands of southern Brazil have sustained livestock farming for many years; however, decline in pasture areas has led to the loss of genetic diversity of forage species. The development of forage grass cultivars adapted to different environments, will contribute to forming new cultivated pastures or recovering degraded areas. The aim of this study was to assess the herbage accumulation of selected intraspecific hybrid progenies of bahiagrass (P. notatum Flügge) in two different environments (Depressão Central and Campanha region) to estimate their agronomic value. The results indicated variability for the main forage traits studied (leaf dry mass, stem dry mass, inflorescence dry mass, total dry mass, leaf: stem ratio, plant height and population density of tillers). All the hybrids producing more forage mass than the ‘Pensacola’ cultivar in the municipality of Eldorado do Sul and the majority also superior in the municipality of São Gabriel. The production of leaves in the forage was correlated with total dry mass (84%), suggesting that genotype selection for forage mass also selected for production of leaves. The selected hybrids should be indicated for new steps of assessment within the P. notatum breeding program.

The use of forage species adapted to the local environment allows an easier management, and greater production and stability. It also allows the conservation of the natural genetic resources, and the reduction of the costs and risks of production that further result in higher sustainability of the system. Forage quality may not be considered as important as biomass production in many forage systems. However, when forage plants constitute most or all of the ruminant diet it assumes substantially greater importance. Therefore, the goal of this study was to evaluate the crude protein (CP) content of hybrids of P. plicatulum x P. guenoarum, in a region of Southern Brazil in the Pampa biome. The hybrids evaluated were: 10202, 1020104, 102084, 102080, 1020133, 102058, 102069 (P. plicatulum “4PT” x P. guenoarum “Azulão”), 103063, 10308, 103042, 103040, 103061, 103077, 103087, 103093, 103031, 103020, 103084, and 103037 (P. plicatulum “4PT” x P. guenoarum “Baio”). The CP analyzes were performed on the leaves of the genotypes in each harvest. The hybrid 102069 “Azulão” presented the best CP content (16.4%) compared to the other genotypes in both years. These results are encouraging for forage breeding studies with species of the genus Paspalum.

Genetic improvement of native forage species is a sustainable alternative for maximizing livestock production. Paspalum notatum Flügge is the most important forage grass in the native grasslands of southern Brazil, with substantial potential available for further genetic improvement. The objective of this study was to quantify a range of genetic parameters and predict yield gains in a population of P. notatum intraspecific hybrids. Results indicated intraspecific hybrids of P. notatum had high magnitudes of heritability in the broad and average sense of genotype, plus high selective accuracy and genetic variation for all forage characteristics evaluated. This indicated REML/BLUP can contribute useful information for plant selection in future plant breeding programs. The genetic material studied showed high genetic variability for forage production. Analysis indicated hybrids 336, 332, 437, 132 and male parent ‘30N’ should be included in new crosses to increase the dry matter production of P. notatum. Parents need to be selected from different groups in order to maximize genetic variability and heterosis. In addition, these parents must be included in diallel crosses. The results obtained in this study provide important information for the future breeding of improved P. notatum cultivars for commercialization.

HIGHLIGHTS Low genetic similarity in Paspalum notatum accessions. High genetic distance among diploid accessions. The accessions have good potential to breeding program.

This study evaluated the mode of reproduction, the meiotic behavior and the pollen viability of three tetraploid plants (2n=4x=40) originated from somatic chromosome duplication of Paspalum notatum plants. The plant WKS 3 changed the mode of reproduction after duplication and became apomictic. The plants WKS 63 and WKS 92 confirmed sexual mode of reproduction identical to that of the original genotype. The analyzed plants presented meiotic abnormalities related to tetraploidy, and the chromosome pairing were variable, but it did not hinder the meiotic products, which were characterized by regular tetrads and satisfactory pollen fertility, ranging from 88.7 to 95.7%. Results show that all plants are meiotically stable and that they can be used in intraspecific crosses in the breeding program of Paspalum notatum.

ABSTRACT: The goal of this study was to assess the variability of Paspalum plicatulum x P. guenoarum interspecific hybrids in terms of agronomic performance and their tolerance to cold conditions, as well as to estimate the correlation of different phenotypic characters associated with forage production. Twenty hybrids plants were used, besides one access P. guenoarum , one of P. plicatulum and the cultivar 'Pensacola' ( P. notatum ). Assessments were performed on individual plants, in a completely randomized design with five repetitions. Paspalum genotypes presented variability in total dry matter, leaf dry mass, leaf: stem ratio, and tolerance to cold. We suggest selection of the hybrids 08Q01 and 08Q44 for new stages within a forage improvement program. Estimates of phenotypic correlation may aid in the selection of genotypes with better agronomic traits.

Condensed tannins are formed by monomers of procyanidins and prodelfinidins, where the proportion and concentration of their monomers varies according to the plant species and environmental conditions. In Lotus spp., condensed tannins prevent tympanism in ruminants that feed on them. This study aimed to evaluate the concentration of procyanidins and their monomers, catechin and epicatechin in the genotypes of Lotus L. grown in soil with different saturations of aluminum. A two-factor (genotype × Al saturation) assay was performed, where the genotypes São Gabriel, Ganador, and UFRGS (Lotus corniculatus L.); Serrano (Lotus uliginosus); and El Rincón (Lotus subbiflorus) were cultivated in soil with an Al saturation of 0-20%. The procyanidins were evaluated using high-performance liquid chromatography, which was previously validated for catechin and epicatechin. The concentration of procyanidins and the proportion of epicatechin:catechin were affected by the genotype × environment interaction. In L. corniculatus and L. subbiflorus, the concentration of procyanidin was significantly higher when they were grown in the soil with an Al saturation of 20% compared to that when they were grown in the soil with 0% Al saturation, but the opposite effect was observed in L. uliginosus. The proportion of epicatechin:catechin decreased in plants grown in soil without Al, and only the UFRGS genotype maintained a similar proportion under both the soil acidity conditions. The predominant monomer was epicatechin, which varied from 57% to 75% according to the soil in which the plants were grown.

The Cynodon spp. collection maintained by United States Department of Agriculture National Plant Germplasm System (USDA-NPGS) has limited information on nutritive value (NV) traits. In this study, crude protein (CP), phosphorous concentration (P), in vitro digestible organic matter (IVDOM), and neutral detergent fiber (NDF) were determined to (i) estimate genetic parameters for NV, (ii) obtain genetic values for the whole population across two harvests, (iii) estimate genotype by harvest interaction (GHI) for NV traits, and (iv) select accessions exhibiting improved NV traits compared to ‘Tifton 85′. The experiment was setup as a row-column design with two replicates and augmented representation of controls: Tifton 85, ‘Jiggs’, and ‘Coastal’. The whole-population was harvested twice, and data were analyzed using linear mixed models with repeated measures. In addition, a selected population of 15 genotypes were evaluated across 11 harvests to determine the extent of GHI. Genetic parameters revealed the presence of significant genetic variability, indicating potential improvements for NV through breeding. Specifically, P and IVDOM presented large variation, while NDF had lower diversity but some accessions exhibited lower NDF than Tifton 85. Low GHI, except for IVDOM, indicated genotypic stability and potential for selecting improved accessions under fewer harvests. Breeding line 240, PI-316510, and PI-3166536 presented superior NV than Tifton 85.

The objective of this study was to analyze the adaptability and the stability in apomictic genotypes of the genus Paspalum across the main characters of forage interest evaluated in different years and locations. The experiment was conducted in the years 2010 and 2011 in the municipalities of Eldorado do Sul and Augusto Pestana, RS, Brazil, in a randomized complete block design with three replications. Analyses of adaptability and stability were calculated by the Traditional and the Eberhart and Russel method. The genotypes of P. guenoarum Baio anda Azulão shows high biomass production but adapted to favorable environments and reduced stability. The genotypes of P. lepton 28C show the total dry mass specific adaptability to unfavorable environment and in the dry leaf adaptability with stability

Paspalum notatum is one of the most important native grassland species of southern Brazil, and the main forage source for livestock. The native ecotypes are tetraploid and have apomictic reproduction, which prevents the registration and protection of plant varieties. However, the cultivated variety Paspalum notatum var. saurae (cultivar Pensacola) is diploid and sexual. The objective of this study was to artificially produce polyploid cv. Pensacola plants, to be used as female parents in crosses with male tetraploid and apomictic parents. Seedlings and seeds were immersed in colchicine solutions for different periods. A tetraploid chromosome number was confirmed in three plants. The reproduction mode of the resulting polyploid plants will be assessed; if confirmed to be sexual, they can be used in crosses with native apomictic plants to breed new intraspecific hybrids.

ABSTRACT Foliar diseases impose severe restrictions on the persistence and productivity of Medicago sativa, both of which may be increased by developing disease resistant and more competitive genotypes that can improve pasture quality. We found Curvularia geniculata as the principal alfalfa foliar pathogen in the Brazilian state of Rio Grande do Sul (RS). Growth chamber experiments evaluated the resistance of alfalfa genotypes ‘E1C4’, ‘CPPSul’, ‘ABT 805’ and ‘CUF-101’ to C. geniculata as compared the control ‘Crioula’ genotype. These genotypes were also evaluated in field trials at a sea level site in Eldorado do Sul in central RS and at two sites £200 m above sea level, one in Bagé municipality in south west RS and the other at a farm near the town of Alto Feliz in north east RS. Plants were spray-inoculated with 1.6 x 106 ml-1 of C. geniculata spores and visually evaluated for leaf damage 14 days later. The C. geniculata infection rates varied from zero to 100%. Alfalfa persistence and forage mean dry mass (DM) production at the Eldorado site were measured during different seasons from November 2013 to January 2015 by calculating the incidence of invasive plants and morphologically separating leaves from stems and calculating both leaf and stem DM. Data were analysed using mixed statistical models. The best results for persistence and forage DM were shown by the ‘CPPSul’ genotypes (DM = 16,600 kg ha-1) and ‘Crioula’ (DM = 15,750 kg ha-1). These two genotypes will be used for subsequent investigations and selection cycles.

ABSTRACT The Paspalum genus includes several species that are important for livestock in Rio Grande do Sul, such as P. notatum and P. guenoarum, typical of native pastures of the Pampa biome. The aim of this study was to investigate forage production and chemical composition of four ecotypes of these species in relation to the cv. ‘Pensacola’ (P. notatum). Ecotypes of P. guenoarum (Azulão and Baio) and P. notatum (André da Rocha and Bagual) and the cv. ‘Pensacola’ were evaluated for two years, during which four cuts/year were made. The work was carried out under field conditions at the Agronomic Experimental Station of the Federal University of Rio Grande do Sul (30°05’S; 51°39’W), in a completely randomized design. P. guenoarum stood out for higher productivity and greater tolerance to cold; the Azulão ecotype showed more autumn production in relation to the other ecotypes. Crude protein content ranged from 14 (Baio) to 15% (‘Pensacola’); for neutral detergent fiber, the variation was 68 (Azulão) to 71% (‘Pensacola’) and for acid detergent fiber there was a variation of 38 (‘Pensacola’) to 43% (Baio). The data demonstrates the potential of native genotypes for use as cattle feeding in southern Brazil.

O objetivo deste trabalho foi determinar a variabilidade genética e a expressão de caracteres de interesse forrageiro em espécies de Paspalum. Os experimentos foram conduzidos em diferentes locais e anos de cultivo, em delineamento de blocos ao acaso, com três repetições. Foram avaliados cinco acessos de P. nicorae e dois de P. guenoarum, além da cultivar Pensacola (P. notatum), utilizada como testemunha. Foram quantificados os seguintes caracteres: relação folha/colmo, índice de colheita e massa de matéria seca total, de folhas e de colmo. Tanto os efeitos principais (genótipos, anos e locais de cultivo) quanto a interação entre os fatores tiveram influência significativa sobre os caracteres avaliados. Os acessos avaliados apresentam variabilidade genética em caracteres de interesse forrageiro, bem como desempenho variável de acordo com o local e o ano de cultivo. A produção de matéria seca total e de folhas são os caracteres que mais contribuem para a detecção da variabilidade genética observada, independentemente do ano de avaliação.

Twenty eight natural populations of Trifolium riograndense Burkart, an important forage legume from native pastures of the state of Rio Grande do Sul, Brazil, were evaluated for genetic diversity with eight Simple Sequences Repeats (SSR) markers. Chromosome numbers were also determined. The eight markers were polymorphic, with 35 alleles and an average of 4.37 alleles per locus, and Polymorphism Information Content (PIC) between 0.48 and 0.80. Group analysis based on Jaccard´s similarity coefficient separated the 28 accessions in nine groups, with an average genetic similarity of 0.44, indicating a high genetic variability among the populations. No evident relation between genetic distance and geographical origin was detected. The chromosome number of 2n=2x=16 was found in all populations, indicating lack of intraspecific variability for chromosome number in the species. This information on diversity can be used in conservation strategies as well as in genetic breeding programs of this species.

Realizou-se neste estudo uma avaliação inicial em diversas linhagens de aveia-branca (Avena sativa, L.), com o intuito de verificar o desempenho agronômico e identificar os genótipos promissores para o lançamento de cultivares para produção de forragem. Os experimentos foram conduzidos em duas regiões fisiográficas do estado do Rio Grande do Sul, Depressão Central e Campanha Gaúcha, nos anos de 2008 e 2009. Foram implementados em blocos casualizados com quatro repetições. Cada parcela foi constituída por uma linha de 2 m de comprimento, espaçadas a 40 cm entre si, considerando parcela útil o metro linear central, cortadas a uma altura de 10 cm. Foram avaliadas 21 linhagens de aveia-branca mais a testemunha (IPR126). No primeiro ano, também foram avaliadas duas linhagens de triticale, as quais apresentaram baixo potencial forrageiro e foram excluídas dos experimentos no segundo ano. Na avaliação do potencial produtivo das linhagens de aveia-branca, não houve diferenças ocasionadas pela interação dos fatores local, ano e linhagem. No primeiro ano, os valores médios de produção na Depressão Central e Campanha Gaúcha foram de 82,3 e 76,3 g por metro linear de matéria seca total, respectivamente. Na avaliação do segundo ano, as médias de produção para esta espécie na Depressão Central e na Campanha Gaúcha foram, respectivamente, 80,0 e 119,8 g m-1 de matéria seca total. As linhagens de aveia-branca, em sua maioria, apresentam bons resultados e são promissoras para o programa de melhoramento genético dessa espécie.The objective of this study was to proceed with an initial evaluation of various strains of white oats (Avena sativa, L.), with the objective of characterizing the genetic diversity and the agronomic performance, identifying the promising genotypes for the release of cultivars, with the purpose of forage production. The experiments were conducted in two geographical regions of the State of Rio Grande do Sul, Depressão Central and in the Campanha Gaúcha, Brazil, in the years 2008 and 2009, in a randomized block design with four replications. Each plot consisted of a 2-m line, with lines spaced at 40 cm. The central linear meter was considered as useful plot, cut to a height of 10 cm. Twenty-one oat lines were evaluated, plus the control (IPR126). In the first year, two strains of triticale were also studied, and had a low potential for forage and were excluded from the experiments in the second year. The productive potential of oat strains did not differ for the interaction of location, year or lineage, with the first year of average production in the Depressão Central and Campanha Gaúcha, of, 82 3 and 76.3 grams per meter of total dry matter (TDM), respectively. At the second year evaluation, the means of production for this species in the Depressão Central and Campanha Gaúcha were 80.0 and 119.8 g m-1 TDM. Most strains of oats present good results and are promising for the breeding program of this species.

Este trabalho foi realizado com o objetivo de selecionar e caracterizar genótipos de Lotus corniculatus com respostas contrastantes à toxidez por alumínio. Quatro cultivares (São Gabriel, San Gabriel, Draco, Estanzuela) e uma população (UFRGS) foram submetidas a duas seleções consecutivas em solos com 30-35% de saturação por alumínio. As seleções consistiram da separação de 1% dos indivíduos mais e menos vigorosos para obtenção de genótipos tolerantes e sensíveis, respectivamente. Os indivíduos selecionados foram comparados ao germoplasma de origem por avaliação agronômica. Os genótipos selecionados pela tolerância ao alumínio tóxico foram de modo geral superiores aos germoplasmas originais, enquanto aqueles selecionados pela sensibilidade parecem ter sido selecionados pelo menor vigor, pois foram inferiores ao germoplasma original, mesmo em situações de ausência de alumínio tóxico. Entre os genótipos selecionados, destacou-se o UFRGS selecionado para tolerância, que apresentou elevada produção de matéria seca, sendo superior aos demais genótipos. A realização de testes a campo e a multiplicação de sementes destes genótipos para posterior uso em áreas que apresentem esta limitação é necessária, particularmente para a população de UFRGS selecionada para tolerância ao alumínio.The objective of this study was to select and characterize Lotus corniculatus genotypes with contrasting responses to toxicity by aluminum. Four cultivars (São Gabriel, San Gabriel, Draco, Estanzuela) and one population (UFRGS) were submitted to two consecutive selections in soils that presented 30-35% aluminum saturation. Selections consisted of separating 1% of the most and less vigorous individuals in order to obtain tolerant and sensitive genotypes, respectively. The individuals chosen were compared with the original germplasm by agronomical evaluation. Genotypes selected by tolerance to toxic aluminum were overall superior to the original populations. However, the materials chosen by sensitivity were inferior to the original populations even in situations where toxic aluminum was absent. Among the selected genotypes, the UFRGS selected for tolerance stood out, presenting an elevated dry matter production level - superior to all other genotypes. Field testing and seed multiplication of these genotypes for further use in areas that present this limitation is necessary, especially for the UFRGS population selected for tolerance to aluminum.

O objetivo neste trabalho foi selecionar genótipos mais produtivos e persistentes de trevo-branco (Trifolium repens L.) por meio da avaliação agronômica de suas progênies. Foi avaliada a produção de matéria seca (MS) de 22 progênies comparadas à população UFRGS-2004-2 e à cultivar Jacuí de trevo-branco consorciadas com azevém (Lolium multiflorum Lam.) durante os anos de 2008 e 2009. A média de produção de MS de trevo-branco durante os dois anos foi de 3.671 e 2.107 kg/ha em Eldorado do Sul e Bagé, respectivamente. Em Eldorado do Sul e Bagé, a cultivar Jacuí com mais 13 progênies formaram os grupos com maior produção. Sete progênies ficaram no grupo "a" em ambos os locais, enquanto somente a população UFRGS e uma progênie apresentaram menores produções nos dois locais, havendo interação genótipo × ambiente. As progênies 2, 6 e 7 oriundas de Bagé e 22 (Etiópia), 23 (Portugal), 24 (Estados Unidos) e 30 (Irlanda) podem ser utilizadas na formação de uma cultivar sintética mais produtiva e persistente as condições do verão.The aim of this work was to select higher yielding and persistent genotypes, evaluating their progenies agronomically. The DM yield of 22 progenies compared with the population of UFRGS-2004-2 and with the Jacuí cultivar of white clover mixed with ryegrass (Lolium multiflorum Lam.), during the years 2008 and 2009 was evaluated. The average DM yield of white clover during the two years was 3671 and 2107 kg/ha, in Eldorado do Sul and Bagé, respectively. In Eldorado do Sul and Bagé the Jacuí cultivar with thirteen more progenies formed groups with higher production. Seven progenies were in the group "a" in both places, whereas only the population of UFRGS and one progeny had lower production in both sites, with genotype × environment interaction. Progenies 2, 6 and 7 from Bage and 22 (Ethiopia), 23 (Portugal), 24 (United States) and 30 (Ireland), can be utilized in the creation of a more productive and synthetic cultivar, in addition to being more resistant to summer conditions.

O trevo branco é uma leguminosa forrageira muito importante para utilização em pastagens temperadas no Rio Grande do Sul. Entretanto, ele é mais sensível ao déficit de água no solo do que as outras leguminosas perenes, apresentando problemas de persistência no verão. Com o objetivo de determinar características morfofisiológicas relacionadas à resposta ao déficit hídrico dessa espécie, foi realizado um experimento em casa-de-vegetação, o qual avaliou o efeito da disponibilidade hídrica (90 e 40% da umidade de capacidade de campo do solo) sobre sete acessos pertencentes à Coleção Nuclear de Trevo Branco do Departamento de Agricultura dos Estados Unidos (USDA). O delineamento experimental utilizado foi inteiramente casualizado, com quatro repetições, totalizando 56 vasos. Foram realizadas duas avaliações, aos 130 e aos 196 dias após a semeadura, ocasião em que foram avaliadas diversas características morfofisiológicas. A análise dos resultados indicou que o déficit hídrico teve um efeito marcante sobre a altura de plantas (EST), área foliar (AF), número de folhas vivas (NFV), comprimento de estolão (CES), produção de massa seca (MSPA), taxa fotossintética (A), condutância (g) e eficiência do uso da água (EUA). Nas duas avaliações, em ambas as disponibilidades hídricas, verificaram-se correlações positivas e significativas (PThe white clover is a forage legume very important for use in temperate pastures in Rio Grande do Sul. However it is more sensitive to water deficit in the soil than other perennial legumes, presenting a lack of persistence in the summer. With the objective of determining morphophysiological traits related to the answer to the water deficit of this species, an experiment was carried out in greenhouse, which evaluated the effect of water availability (90 and 40% of the soil moisture field capacity) on seven genotypes belonging to the white clover core collection from the United States Department of Agriculture (USDA). The experimental design used was completely random, with four repetitions, totaling 56 pots. Two evaluations were made, at 130 and 196 days after the sowing, when several traits were measured. The analysis of the results indicated that the water deficit had an outstanding effect on most of the variables, such as plant height (PH), foliar area (FA), number of alive leaves (NAL), length of primary stolon (LPS), dry matter of the aerial part (DMAP), photosynthesis rates (A), conductance (g), and water use efficiency (WUE). In both evaluations, regardless the water availability, there were positive and highly significant correlations (P

The objective of this work was to evaluate the response of five birdsfoot trefoil populations (Lotus corniculatus L.), selected under severe and intense grazing (P38, P37, P9 and P3) or cutting (population Corte), one rhizomatous population from Morocco and two cultivars, São Gabriel (Brazil) and ARS 2620 (USA), to different cutting intervals (20 and 40 days). The trial was carried out in a greenhouse for 224 days. The plants were submitted to the treatments during four months, when it was made four and two cuttings for the 20 and 40 day intervals, respectively. In the following months, aerial part of the plants was totally removed and after 70 days of regrowth, evaluation of roots and aerial section were performed. Analysis of variance and multivariate analysis was performed, obtaining the Mahalanobis distance (Md), dendogram by UPGMA method and the relative contribution of the characters for genetic divergence. There was no genotype × cutting interval interaction and the genotypes presented a reduction on dry matter yield of roots, crown and aerial sections, number of stems and plant height when submitted to frequent cuttings. The most divergent genotypes were Marrocos and P9 (Md = 108.7) and the most similar ones were São Gabriel and P37 (Md = 7.8). The results suggest exclusion of the population P9 because of its weak performance and the utilization of the populations Corte and P37 as progenitors in the birdsfoot trefoil breeding program. Root dry matter accumulation and plant height were the characters that contributed most to genetic divergence and they can be used for selection works.

Objetivou-se com este trabalho caracterizar espécies diploides, inclusive a espécie modelo Lotus japonicus, e linhas endogâmicas recombinantes do gênero Lotus, quanto à tolerância ao alumínio (Al) tóxico utilizando-se solo ácido e solução nutritiva. Os experimentos foram conduzidos em casa-de-vegetação, sendo testadas três espécies diploides (L. japonicus MG-20 e GIFU, L. filicaulis e L. burtii) e 180 linhas endogâmicas recombinantes. Nos experimentos com espécies diploides, utilizou-se a alfafa como testemunha sensível e, nos experimentos com linhas endogâmicas recombinantes, utilizou-se a espécie modelo GIFU. Nos experimentos em solo, foram avaliadas características morfológicas da parte aérea e da raiz e, nos experimentos em solução nutritiva, apenas o comprimento e crescimento radicular. As espécies modelo MG-20 e GIFU foram, em geral, as mais produtivas. Os resultados em solução nutritiva seguiram padrão de resposta semelhante ao observado nas avaliações em solo ácido, sendo a espécie MG-20 superior às demais em todas as concentrações de alumínio testadas. Das 180 linhas endogâmicas recombinantes testadas, 24 foram superiores e 39 inferiores à espécie GIFU. A grande diversidade observada nas espécies modelo e nas linhas endogâmicas recombinantes pode auxiliar na futura seleção de genótipos cultivados (tetraploides), uma vez que essas espécies possuem um grupo de marcadores moleculares desenvolvidos que podem ser utilizados na identificação de regiões responsáveis pela maior ou menor tolerância à toxidez por alumínio.The objective of this work was to characterize diploid species, including Lotus japonicus model species and recombinant inbred lines of the Lotus genus for resistance to aluminum (Al) toxicity by using acid soil and nutrient solution. The experiments were conducted in greenhouse, being tested three diploid species (L. japonicus MG-20 and GIFU, L. filicaulis and L. burtii), and 180 recombinant inbred lines. In the experiments with diploid species, alfalfa was used as a check, and in the experiments with recombinant inbreed lines, GIFU model species was used. In the soil experiments, the morphological characteristics of the aerial part and the root were evaluated, and in the nutrient solution experiment, only the root length and growth were evaluated. MG-20 and GIF model species were the most productive in general. The results in nutrient solution followed the response pattern similar to the one observed in acid soil evaluations. The MG-20 genotype was superior to the others in all Al concentrations tested. Of the 180 recombinant inbred lines tested, 24 were superior and 39 inferior to the GIFU species. The great diversity observed in the model species and in the recombinant inbred lines can help in future selection of cultivated genotypes (tetraploids) inasmuch as they have a group of developed molecular markers which can be used at identification of regions responsible for higher or lower tolerance to aluminum toxicity.

Este estudo foi realizado para avaliar uma coleção de 53 acessos de Paspalum nicorae, estabelecidos em duas regiões fisiograficamente distintas durante dois anos, buscando caracterizar a produção e qualidade de forragem dos acessos para etapas subsequentes em programa de melhoramento de plantas forrageiras. Os acessos foram estabelecidos em linhas, num delineamento de blocos casualizados com três repetições, e as avaliações foram realizadas de outubro de 2007 a fevereiro de 2009 por meio de cortes. Houve variação da produção de forragem entre os acessos ao longo do tempo e entre os locais. A maioria dos acessos de P. nicorae apresentou elevado rendimento de forragem em comparação à testemunha (P. notatum cv. Pensacola) e alguns acessos apresentaram desempenho bem expressivo. Os percentuais de proteína bruta obtidos nos acessos da coleção foram semelhantes aos encontrados na testemunha. Os acessos 28B e 26A de P. nicorae são indicados para etapas subsequentes de um programa de melhoramento de plantas forrageiras.This study was carried out to evaluate a collection of 53 accessions of Paspalum nicorae, introduced in two physiographically distinct regions over two years, with the objective of characterizing production and quality of forage of access for subsequent phases of a forage plant breeding program. The accessions were established in lines in randomized block design with three replications and the evaluations were performed from October 2007 to February 2009 by using cuts. There was variation of forage production among access over time and among sites. The majority of the accessions of P. nicorae presented high forage yield when compared with the control (P. notatum cv. Pensacola) and some accessions presented very expressive performances. The percentages of crude protein obtained from accessions in the collection were similar to the ones found in the control access. The accessions 28B and 26A of P. nicorae are indicated for subsequent stages in a program of forage plant breeding.

Several species of Trifolium are widely cultivated as forage for animals and green manure. This work aimed to characterize and estimate through morphological and agronomic traits the genetic diversity of natural populations of T. riograndense, an important forage legume from native pastures of the state of Rio Grande do Sul, Brazil. Plant height and diameter, number of primary and secondary stolons, stolon length, number of nodes per primary stolon, internode length, primary stolon diameter, leaf area, petiole length and dry matter production were analyzed in 29 accessions. The first five attributes and dry matter production were evaluated twice. A broad phenotypic variability was observed for almost all characteristics. Positive correlations occurred between petiole length and leaf area (r = 0.68), petiole length and plant height in the first evaluation (0.63), stolon length and plant height (0.58), total dry matter and plant diameter (0.61), and total dry matter and plant height in the second evaluation (0.55). The two most divergent accessions were collected in the same physiographic region and the two less divergent ones in different regions. A dissimilarity dendrogram separated four groups. Accessions collected in a region where T. riograndense is abundant were found to belong to different groups outlining the great variability of this native forage legume. Dry matter production in the first cut was the characteristic that most contributed (20.80%) to accessions divergence followed by number of secondary stolons (12.30%), leaf area (11.07%), and number of nodes per primary stolon (10.93%).Várias espécies de Trifolium são extensivamente cultivadas como forragem ou adubo verde. Caracterizaram-se e estimaram-se, por meio de atributos morfológicos e agronômicos, a diversidade genética de populações naturais de T. riograndense, importante leguminosa forrageira nativa dos campos naturais do Rio Grande do Sul. Estatura e diâmetro das plantas, número de estolões primários e secundários, comprimento do estolão primário, número de nós por estolão, comprimento dos entrenós, diâmetro de estolão, área foliar, tamanho do pecíolo e produção de massa seca foram avaliados em 29 acessos. Os cinco primeiros atributos e a produção de massa seca foram avaliados duas vezes. Ampla variabilidade fenotípica foi observada para quase todas as características. Ocorreram correlações positivas entre tamanho do pecíolo e área foliar (r = 0,68), tamanho do pecíolo e estatura (0,63) na primeira avaliação, e comprimento do estolão e estatura (0,58), massa seca total e diâmetro da planta (0,61) e massa seca total e estatura (0,55) na segunda avaliação. Os dois acessos mais divergentes foram coletados na mesma região fisiográfica e os dois menos divergentes em regiões diferentes. O dendrograma de dissimilaridade genética separou quatro grupos. Acessos coletados em uma região onde T. riograndense é abundante pertenciam a diferentes grupos, o que realça a grande variabilidade dessa leguminosa forrageira nativa. A produção de massa seca no primeiro corte foi a característica que mais contribuiu (20,80%) para a divergência dos acessos, seguida pelo número de estolões secundários (12,30%), área foliar (11,07%), e número de nós por estolão (10,93%).

O experimento foi realizado com o objetivo de selecionar genótipos de Lotus corniculatus L. (Draco, São Gabriel e UFRGS) mais tolerantes ao alumínio, utilizando-se a técnica de seleção em solução nutritiva. A solução continha 200 µmol/L de cálcio (CaCl2), 100 µmol/L de alumínio (AlCl3) e pH controlado na faixa de 4,1 a 4,3. Realizaram-se dois ciclos de seleção, nos quais as plântulas foram selecionadas pelo comprimento final das radículas. Posteriormente, avaliou-se o ganho genético alcançado nos ciclos de seleção, tanto nas populações originais como nas melhoradas, em um experimento com solução nutritiva com quatro concentrações de alumínio (0, 50, 100 e 150 µmol/L) (AlCl3), por meio do crescimento radicular líquido. O uso de solução nutritiva foi eficiente na seleção de plântulas de cornichão tolerantes ao alumínio. Os materiais analisados apresentam diferenças em relação a esta característica, destacando-se o genótipo UFRGS F2, proveniente de dois ciclos de seleção, como o material com maior tolerância ao alumínio.The experiment was carried out to select genotypes of Lotus corniculatus L. (Draco, São Gabriel and UFRGS) more tolerant to aluminum, using the technique of selection in nutrient solution. The solution contained 200 µmol/L calcium (CaCl2) and 100 µmol/L aluminum (AlCl3) and a pH controlled in the range from 4.1 to 4.3. It was performed two cycles of selection, in which the seedlings were selected by the root final length. Subsequently, the genetic gain achieved in the cycles of selection was tested, in original and improved populations in an experiment in nutrient solution with four concentrations of aluminum (0, 50, 100 and 150 µmol/L) (AlCl3), by liquid root growth. Use of nutritional solution was efficient in the selection of seedlings of birdsfoot trefoil tolerant to aluminum. The materials analyzed differ for this characteristic, with the genotype UFRGS F2, being the most tolerant.

Este trabalho foi conduzido com o objetivo de avaliar o potencial de produção de sementes de 25 acessos da coleção básica de trevo-branco provenientes do Departamento de Agricultura dos Estados Unidos. Por meio de amostragens semanais realizadas entre 6/11/2003 e 10/3/2004, foram avaliadas as seguintes variáveis: número de inflorescências/planta, número de flores/inflorescência, número de inflorescências maduras/planta, número de legumes maduros/inflorescência, peso de mil sementes e rendimento de sementes/planta. O delineamento experimental utilizado foi o completamente casualizado, com os acessos arranjados individualmente em cinco repetições. O rendimento de sementes em trevo-branco é altamente influenciado pelo número de inflorescências por planta, pelo número de inflorescências maduras por planta e pelo peso de mil sementes. Os acessos 53, 2 e 20 destacam-se pela superioridade em relação aos demais (7, 68, 19, 79, 58, 3, 15, 75, 64, 50, 33, 13, 59, 38, 28, 80, 54, 29, 31, 23, 22, 27, 65 e 73) na produção de sementes. Os acessos 27, 65 e 73 não produzem sementes nas condições locais durante o primeiro ano de avaliação.This work was carried out to evaluate the potential of seed production of 25 accessions of the basic collection of white clover from the United States Department of Agriculture. Through weekly samplings performed from 11/6th/2003 to 3/10th/04 the following variables were analyzed: number of inflorescence/plant, number of flowers/inflorescence, number of mature inflorescence/plant, number of mature legumes/inflorescence, weight of 1000 seeds, and seed yield/plant. It was used a complete randomized experimental design with the accessions individually arranged in five replications. Seed yield of white clover is highly affected by number of inflorescence/plant, number of mature inflorescence/plant, and weight of 1000 seeds. The 53, 2 and 20 accessions differ from the others because of their superiority (7, 68, 19, 79, 58, 3, 15, 75, 64, 50, 33, 13, 59, 38, 28, 80, 54, 29, 31, 23, 22, 27, 65 and 73) of seed production. The accessions 27, 65 and 73 do not produce seeds in the local conditions during the first year of evaluation.

Paspalum nicorae Parodi is a perennial, apomictic tetraploid forage species, native in the state of Rio Grande do Sul, Brazil, with grazing tolerance and adapted to sandy soils and moderate droughts. As a first step to future breeding, a morphological characterization regarding leaf hairiness, sheath, leaf and central venation color, growing habit, plant height, raceme number and length, and leaf length and width was performed in 53 accessions from Rio Grande do Sul that are part of a Paspalum species breeding program. 35.84% of the accessions had completely hairy leaves, 73.58% had green sheath, 54.71% whitish central venation, 50.94% decumbent habit. Leaf color was estimated by two methods, color chart and colorimeter. The accessions were classified as 76.92% green, 13.45% greenish yellow and 9.62% as grayish green by the first, and 59.62% grayish, 32.69% grayish yellow, 5.77% yellow and 1.92% dark grayish by the second method. Raceme length ranged from 9.40 to 1.30 cm, number of racemes from one to six (48.73% of the accessions had four racemes), leaf length and width from 36.13 to 13.06 cm and 0.67 to 0.36 cm, respectively, and plant height from 115.70 to 29.00 cm. Therefore, a large morphological variation among accessions was detected, with no relation to geographical location but indicating a high potential use in plant breeding programs.Paspalum nicorae Parodi é uma forrageira perene, apomítica, tetraplóide, nativa no Rio Grande do Sul, com tolerância ao pastejo e adaptada a solos arenosos e a secas moderadas. Como passo inicial para o melhoramento, uma caracterização morfológica considerando pilosidade da folha, cor da bainha, da folha e da nervura central, hábito de crescimento, altura da planta, número e comprimento de racemos, comprimento e largura da folha foi realizada em 53 acessos coletados no Rio Grande do Sul, Brasil, que são parte de um programa de melhoramento de espécies de Paspalum. Os resultados mostraram que 35,84% dos acessos tinham folhas completamente pilosas, 73,58% tinham bainha verde, 54,71% venação central esbranquiçada e 50,94% hábito decumbente. A cor da folha foi estimada por dois métodos, cartela de cores e colorímetro Os acessos foram classificados como 76,92% verdes, 13,45% amarelo esverdeados e 9,62% como verde acinzentados pelo primeiro, e 59,62% acinzentados, 32,69% amarelo acinzentados, 5,77% amarelos e 1,92% cinza escuros pelo segundo método. O comprimento do racemo variou de 9,40 a 1,30 cm, o número de racemos de um a seis (sendo que 48,73% dos acessos tinham quatro racemos), o comprimento e a largura da folha de 36,13 a 13,06 cm e 0,67 a 0,36 cm, respectivamente e a altura da planta de 115,70 a 29,00 cm. Portanto, grande variação morfológica foi detectada entre os acessos, sem relação com localização geográfica, mas indicando alta potencialidade de utilização em programas de melhoramento.

Objetivou-se avaliar as características morfológicas de plantas de alfafa relacionadas à aptidão ao pastejo. Foram avaliadas três populações de alfafa Crioula de diferentes regiões geográficas, sem histórico de pastejo: Chile, Roque Gonzales e Vale do Taquari, além de dois cultivares norte-americanos, ABT 805 (selecionado sob pastejo) e CUF 101 (selecionado sob cortes). O experimento foi realizado em parcelas com os diferentes materiais, mantidos sob pastejo, mantendo-se resíduo de pastejo entre 3 e 5 cm de altura durante 255 dias sob lotação contínua. Ao término do período experimental, foi determinada a porcentagem de sobrevivência das plantas, visando estratificar os genótipos quanto ao grau de aptidão ao pastejo. O cultivar ABT apresentou a maior porcentagem de sobrevivência e o cultivar CUF, a menor. As populações crioulas Chile e Vale do Taquari apresentaram sobrevivência intermediária, enquanto a Roque Gonzales teve sobrevivência similar à do cultivar CUF. Os resultados da caracterização morfológica não diferiram quanto ao crescimento contrátil da coroa, à área da coroa, à área foliar, à área foliar específica, ao número de hastes por planta e ao tipo de haste por planta (basilar ou axilar). A relação folha:caule foi melhor no cultivar ABT e foi influenciada pela menor massa de hastes por planta. A expressão do número de gemas por unidade de carbono alocada às hastes foi maior no cultivar ABT, constituindo-se um eficiente descritor funcional da aptidão ao pastejo, denominado gema específica.The morphological characteristics of alfalfa plants associated to the grazing aptitude were evaluated in this trial. Three Crioula alfalfa populations originated from different regions without grazing history were evaluated: Chile, Roque Gonzales and Vale do Taquari, besides two North American cultivars, ABT 805, selected under grazing and CUF 101, selected under mowing. The trial was composed of plots with different materials, kept under grazing, where the grazing residue was constantly kept at 3-5 cm of height during a 255-day period under continuous stock. At the end of the experimental period, the plant survival percentage was calculated, which served as a parameter to stratify the genotypes in relation to the grazing adaptation level. ABR cultivar presented the highest survival percentage and CUF cultivar the smallest. Crioula populations Chile and Vale do Taquari had intermediate, while Roque Gonzales had survival rate similar to that of CUF cultivar. The morphological characterization results did not show differences in relation to the crown retractile growth, crown area, leaf area, specific leaf area, number of stems per plant and type of stem per plant (basilar or axial). Differences in relation to the stem/leaf ratio were observed for ABT cultivar, influenced by the smaller mass of stems per plant. The expression of the number of buds per carbon unit allocated to the stems was higher for ABT cultivar, becoming an efficient functional descriptor of the grazing aptitude, called as specific bud.

Neste trabalho objetivou-se selecionar as melhores populações de cevadilha vacariana (Bromus auleticus) mediante avaliação do seu desempenho agronômico e de caracteres morfológicos relacionados à persistência. Oito populações oriundas de quatro municípios do Rio Grande do Sul (Santana do Livramento: A5, A6, A23 e A26; Uruguaiana: A7, A8; Júlio de Castilhos: A17; Quaraí: A27) foram cultivadas em linhas de 5 m, contendo entre 12 e 15 plantas. O transplante foi realizado no dia 16/6/2004 e as avaliações, iniciadas aos 125 dias após o transplante. As plantas foram avaliadas quanto ao hábito de crescimento, à produção de sementes e de matéria seca (MS) e à composição química nos anos 2004 e 2005. Uma vez que o ciclo de crescimento da espécie é hibernal, não foi possível avaliar no primeiro ano a produção de matéria seca. Durante o outono-inverno do segundo ano, as plantas foram submetidas a cinco cortes em intervalos de 31 a 47 dias. Verificou-se variabilidade interpopulacional quanto a hábito (semi-prostrado a ereto), número de panículas (9 a 200/linha), circunferência (29,3 a 36,5 cm/planta), produção (0,6 a 77,2 g/linha) e peso de 1.000 sementes (4,7 a 6,6 g), produção de MS média por corte (137 a 421 g/linha) e total (686 a 2105 g/linha), proteína bruta (14,3 a 21,8%), fibra em detergente ácido (21 a 28,3%), fibra em detergente neutro (53,4 a 58,9%) e hemicelulose (25 a 33,2%). As populações A5, A23 e A26, pelo melhor desempenho agronômico, e a população A8, pela presença de rizomas, são indicadas para continuidade do programa de melhoramento da espécie.The objective of this work was to select the best bromegrass populations (Bromus auleticus) by analyzing their agronomic performance and morphological characteristics linked to persistence. Eight populations originated from four different municipalities from the state of Rio Grande do Sul (Santana do Livramento: A5, A6, A23 and A26; Uruguaiana: A7, A8; Júlio de Castilhos: A17; Quaraí: A27) were cultivated on 5-m rows containing from 12 to 15 plants. The plants were transplanted on 06/16/2004, and evaluations started 125 days transplantation. The plants were evaluated in relation to growth habit, seed and dry matter (DM) production and chemical composition during the years of 2004-2005. Due to the winter growth habit of this species, it was not possible to evaluate the DM production on the first year. During the fall-winter period of the second year, the plants were submitted to five cuts, with intervals from 31 to 47 days. Inter-population variability was observed and related to growth habit (semi-decumbent to tall), number of panicles (9 to 200/row), circumference (29.3 to 36.5 cm/plant), DM production (0.6 to 77.2 g/row) and weight of 1000 seeds (4.7 to 6.6 g), mean DM production per cut (137 to 421 g/row), total DM production (686 to 2105 g/row), crude protein (14.3 to 21.8%), acid detergent fiber (21.0 to 28.3%), neutral detergent fiber (53.4 to 58.8%) and hemicellulose (25.5 to 33.25). Populations A5, A23 and A26, due to their better agronomic performance, and population A8 due to the presence of rhizomes, are indicated for further evaluations in the species improvement program.

This work was carried out to characterize the leaf anatomy of eight Bromus auleticus populations collected in Rio Grande do Sul, Brazil, and called A5, A6, A8, A17, A23, A26 and A27. Totally expanded leaves were collected from plants in the vegetative stage and were sectioned and stained with basic fuchsine and alcian blue. A single layer of epidermis with a smooth and fine cuticle on both surfaces, short tricomes, vascular bundles in three sizes, and the presence of cells with lignified walls along the bundles and on the leaf edge were observed in all populations. The number of bulliform cells varied from three to five, with the exception of population A25, which ranged from five to eight cells. Three populations presented long tricomes (A6, A23 and A27) and palisade chlorenchyma (A6, A7 and A26), five had grooves on the leaf surface (A8, A17, A23, A26, A27) and homogeneous chlorenchyma (A5, A8, A17, A23, A27), two presented a large intercellular space in the mesofile (A7 and A17), and two showed a more visible cuticle on the abaxial surface (A7 and A8). These variations could reflect differences in relation to the nutritive value of the populations and be used in plant breeding programs for the genetic improvement of bromegrass.

Este estudo foi realizado com o objetivo de verificar a diversidade genética na coleção de acessos de P. urvillei do Departamento de Plantas Forrageiras e Agrometeorologia (DPFA) da Faculdade de Agronomia (UFRG) visando sua utilização em futuros trabalhos de seleção. Foram avaliados 64 acessos provenientes do Rio Grande do Sul, 1 de Xanxerê, Santa Catarina, três de Curitiba, Paraná, e 1 da Argentina. A diversidade genética foi analisada por meio de marcadores do tipo RAPD e SSR. Utilizaram-se dez primers para marcadores RAPD, o que possibilitou obter 56 bandas polimórficas e 11 grupos no dendrograma com similaridade média de 0,70. Na técnica de SSR, foram utilizados sete primers e obtidas 28 bandas polimórficas, formando sete grupos no dendrograma com similaridade média de 0,66. Ambos os marcadores foram eficientes para o agrupamento de acessos coletados. O uso de maior número de primers para gerar mais bandas polimórficas foi necessário para obtenção de fingerprintsgenômicos dos indivíduos similares. Os dendrogramas gerados neste estudo dão subsídios para futuros cruzamentos de gerações parentais contrastantes ou similares no melhoramento de Paspalum urvillei.The aim of this study was to estimate the genetic diversity among accesses of P. urvillei of Departamento de Plantas Forrageiras e Agrometeorologia (DPFA) of the College of Agronomy - UFRGS and to evaluate their use in selection programs. Sixty four accesses from different cities of the Southern Region of Brazil (Rio Grande do Sul, Santa Catarina and Parana States) and from Argentine were analyzed by RAPD and SSR molecular markers. Ten primers of RAPD markers were used and resulted in 56 polymorphic bands and 11 groups in a dendrogram with average similarity 0.70. Seven primers were used for the SSR technique and resulted in 28 polymorphic bands and seven groups in a dendrogram with average similarity 0.66. Both markers were efficient on grouping the accesses collected across the Southern region. A large number of primers was required to generate additional polymorphic bands to get genomic fingerprints of similar individuals. The dendrograms obtained from this study provided significant information for designing crossing strategies of parental generations in breeding programs of P. urvillei.

Este trabalho foi conduzido com o objetivo de avaliar a produção e a distribuição estacional de populações locais de azevém em comparação ao cultivar comercial Comum-RS. Foram realizados em 2004 dois experimentos em duas regiões diferentes do Rio Grande do Sul: Eldorado do Sul (EEA) e Veranópolis (EEV). A produção de forragem foi avaliada em sete populações de azevém anual, um cultivar uruguaio (LE-284), um da Nova Zelândia (Eclipse) e o cultivar Comum-RS, totalizando dez tratamentos. O delineamento experimental utilizado foi o de blocos casualizados, com quatro repetições. Foram realizados vários cortes, seguidos da separação morfológica e secagem visando à avaliação dos teores de matéria seca de folhas e de matéria seca total. A produção de azevém variou significativamente entre as populações avaliadas, indicando a existência de uma grande variação dentro da espécie, sobretudo quanto à distribuição da produção de forragem ao longo do ciclo de crescimento. Em ambos os locais, constatou-se a existência de populações com rendimento superior aos dos cultivares comerciais avaliados, especialmente o Comum-RS, indicando possibilidade de obtenção de novos cultivares a partir das populações localmente adaptadas.The objective of this work was to evaluate forage production and seasonal distribution of locally adapted ryegrass populations in comparison to commercial cultivars. Two experiments were carried out in two different locations of Rio Grande do Sul (Eldorado do Sul - EEA and Veranópolis - EEV), in 2004. Forage production of seven annual ryegrass populations, one Uruguayan cultivar (LE-284), one New Zealand cultivar (Eclipse) were compared to the commercial cultivar, in a total of ten treatments. The experimental design was a randomized block with four replications. Several cuts were performed, followed by morphologic separation of plant parts and drying in order to evaluate dry matter yield of leaves and total dry matter. Ryegrass production varied considerably among populations, indicating the existence of a great variation among them, especially regarding forage seasonal distribution along the growth cycle. At both locations, superior populations were observed in terms of forage production when compared to commercial cultivars, especially the Comum-RS, indicating the possibility of obtaining new cultivars of ryegrass from the locally adapted populations.

Neste trabalho, objetivou-se verificar a resposta de populações de alfafa à desfolhação. Avaliaram-se duas populações de alfafa Crioula (tipo-feno), selecionada em estádio de plântula de acordo com o comprimento do segundo entrenó (EC = 1,5 cm; EL = 2,5 cm) e uma do cultivar Alfagraze (tipo-pastejo). As plantas foram cultivadas em vasos nos meses de março a agosto de 2005 e submetidas a uma combinação de altura (2 e 8 cm) e freqüência de corte (semanal, quinzenal e mensal) durante 141 dias. Não houve diferença quanto à produção da matéria seca (MS) aérea sob cortes semanais e quinzenais. Com cortes mensais, a população Crioula-EL, com maior altura e quantidade de hastes, superou as demais. Os cultivares Alfagraze e Crioula-EC foram similares quanto à quantidade de MS subterrânea. As populações de alfafa diferiram na resposta ao manejo de corte e apresentaram declínio gradual na MS quando submetidas a cortes mais freqüentes, com maior sobrevivência do cultivar Alfagraze em comparação aos cultivares Crioula-EC e Crioula-EL. O cultivar Alfagraze diferiu das populações do cultivar Crioula e apresentou menor altura, maior diâmetro, maior proporção de hastes da coroa e maior alocação de MS na parte subterrânea, portanto, estas características estão relacionadas ao tipo-pastejo. O comprimento do entrenó da plântula poderia ser utilizado complementarmente para selecionar populações de alfafa com maior aptidão ao pastejo, o que torna possível obter populações com variabilidade de produção de matéria seca e sobrevivência.This work aimed to evaluate the performance of alfalfa populations in response to defoliation. Two populations of alfalfa cv. Crioula (hay-type) and one population of alfalfa cv. Alfagraze (grazing-type) were evaluated, being selected at seedling stage according to the length of the second internode (EC=1.5 cm; EL=2.5 cm). The trial was performed in pots, between February and August/2005. The populations were harvest at two cutting intensities (2 and 8 cm) and three cutting frequencies (weekly, fortnightly and monthly) during 141 days. There was no difference on shoot dry matter (DM) yield between weekly and fortnightly cuttings; the Crioula-EL, under monthly cuttings was the tallest and had higher amount of stems, surpassing the others. There was similarity between the cultivar Alfagraze and Crioula-SI regarding underground DM biomass. Alfalfa populations responded differently to defoliation management, with a gradual decline on DM yield under more frequent cuttings and a higher survival of plants from Alfagraze compared to Crioula-EC and Crioula-EC. Alfagraze cultivar differed from the Crioula populations, presenting shorter plants, higher proportion of stems originate from the crown and a higher allocation of DM to the underground parts, indicating that these characteristics belong to a grazing-type alfalfa. The length of seedling internodes could be used as a complementary morphological marker to select alfalfa populations more adapted to grazing. In this way, it is possible to obtain populations with DM yield variability and survival.

The genetic diversity in the red clover USA core collection was measured by RAPD and isozyme markers,comparing different approaches of isozyme data analysis and comparing the results of RAPD with those of isozymes. A totalof 15 isozyme loci and 114 RAPD fragments were analyzed. Genetic diversity measured with RAPD and isozymes was high.The analyses of isozymes as binary data was highly correlated with the allele-frequency approach. There was no correlationbetween the distance matrices of different markers. Isozyme data grouped the accessions of red clover in four groups. Thevariation evidenced by AMOVA was higher among and within groups in the populations than at the intra-population level.Results showed the high diversity in the red clover core collection and indicated some populations that could be used inbreeding programs of the crop in Brazil.

Several species of the genus Paspalum L. are important forages, due their to quality, productivity and tolerance to environmental stresses. Chromosome numbers, meiotic configurations and pollen fertility were evaluated in a collection of 85 accessions of Paspalum notatum Flügge and in seven accessions of Pensacola (P. notatum var saurae). All P. notatum accessions were tetraploid, with 2n = 4x = 40, except one diploid accession, considered as an escape of Pensacola. All Pensacola plants had 2n = 2x = 20. Meiotic configurations at diakinesis and metaphase I varied among tetraploid accessions, from plants with only bivalents to plants with high frequency of quadrivalents. Pollen fertility varied from 82.5 to 95.9% among diploid accessions and from 72.4 to 97.9% among the tetraploids. Due to the apomictic mode of reproduction of tetraploid P. notatum, meiotic irregularities can be maintained by plants without harming their propagation. At the same time, pollen fertility should be high enough to assure endosperm development, since the species is pseudogamous. Wild diploid P. notatum populations, apart from the endemic P. notatum var saurae, are very rare. From a plant breeding point of view, all the examined tetraploid accessions are potentially male-fertile and could be used as males in crosses.Muitas espécies do genus Paspalum L. são importantes forragens, devido à qualidade, produtividade e tolerância para o stress ambiental. Números cromossômicos, configurações meióticas e fertilidade do pólen foram analisados em uma coleção de 85 acessos de Paspalum notatum Flügge e sete acessos de Pensacola (P. notatum var saurae). Todos os acessos de P. notatum eram tetraplóides, com 2n = 4x = 40, com exceção de um diplóide, considerado como escape de Pensacola. Todas as plantas de Pensacola examinadas tinham 2n = 2x = 20. Foi verificada variação entre os acessos tetraplóides quanto às configurações cromossômicas em diacinese e metáfase I, desde plantas com apenas bivalentes até aquelas com alta freqüência de quadrivalentes. A fertilidade do pólen variou de 82,5 a 95,9% entre os acessos diplóides e de 72,4 a 97,9% entre os tetraplóides. Devido ao modo de reprodução apomítico de P. notatum tetraplóide, irregularidades meióticas podem ser mantidas sem prejuízo da propagação. Ao mesmo tempo, a fertilidade do pólen deve ser suficientemente alta para assegurar a formação do endosperma, já que a espécie é pseudogâmica. Diplóides silvestres de P. notatum, além do endêmico P. notatum var saurae são muito raros. Do ponto de vista do melhoramento, todos os tetraplóides são potencialmente macho-férteis e poderiam ser utilizados como genitores masculinos em cruzamentos.

Trinta e três isolados de Rhizobium sp obtidos a partir de nódulos de cornichão (Lotus corniculatus L.), coletados em diferentes regiões do Rio Grande do Sul, foram caracterizados pela técnica da PCR (reação em cadeia da polimerase), utilizando oligonucleotídeos específicos para a amplificação de seqüências repetitivas Box. Os isolados apresentaram perfis de amplificação distintos entre si e das estirpes de Mesorhizobium loti, SEMIA 806 e SEMIA 816, atualmente recomendadas para a produção de inoculantes para cornichão. O índice de similaridade genética determinado pelo coeficiente de Jaccard variou entre 0,5 e 0,9% indicando uma grande variabilidade entre os isolados. A análise de agrupamento dos isolados demonstrou a formação de três grupos, não tendo sido possível estabelecer relação entre o perfil de amplificação, grupos formados e origem geográfica dos isolados.

Objetivou-se avaliar o efeito da altura (10 e 20 cm) e do intervalo de cortes (30 e 45 dias) na produção de MS, na composição química e na persistência do capim-melador (Paspalum dilatatum Poir. biótipo Virasoro) em condições de campo. As plantas foram cortadas nove e seis vezes, respectivamente, nos intervalos de corte de 30 e 45 dias, no período de setembro de 2003 a junho de 2004. A produção de MS total não diferiu entre as alturas nem entre os intervalos de corte (média de 2.017 g MS/linha). Houve efeito da interação estação de MS ´ altura de corte: não houve diferença entre primavera e verão sob cortes a 10 cm (997 g/linha); na altura de corte de 20 cm, a produção obtida no verão (938 g MS/linha) foi maior que a da primavera (761 g MS/linha), enquanto, a 10 cm, os cortes na primavera proporcionaram maior produção (1.030 vs 761 g MS/linha a 20 cm). No outono, a produção de MS foi muito baixa (125 g MS/linha). A produção de folhas aumentou sob cortes freqüentes (30 dias) e intensos (10 cm). Nos cortes feitos a intervalos de 30 dias, houve maior teor de PB (14,7%) e menores teores de FDA (43,8%) e FDN (67,8%). Os cortes não evitaram o florescimento precoce, intenso e contínuo das plantas, que alocaram, em média, 27% da MS em folhas e 68% em caules+inflorescências.This work aimed to evaluate the effect of the cutting height (10 cm and 20 cm) and cutting interval (30 days and 45 days) in the DM production of dallisgrass (Paspalum dilatatum Poir. biótipo Virasoro) cultivated under field conditions. Plants were cut between September/2003 and June/2004, nine and six times, for the 30 d and 45 d cutting interval, respectively. The total DM yield did not differ among treatments, with an average of 2,017 g DM/row. There was a significant interaction between season and cutting height, with no difference observed between summer and spring at 10 cm height (997 g DM/row); at 20 cm cutting height, however, there was a higher yield on the summer (938 g DM/row) than in the spring (761 g DM/row); during the spring, the 10 cm cutting height enhanced the DM production (1,030 g DM/row) in relation to the 20 cm cutting height (761 g DM/row). The fall DM production was low (125 g DM/row). The leaf yield increased under frequent (30 days) and intense (10 cm) cutting. The highest CP concentration (14.7%) and the lower ADF (43.8%) and NDF (67.8%) concentrations were obtained under the frequency of 30 days. The cutting management did not affect the early, continuous, and intense flowering of the plants, which allocated an average of 27% of DM to the leaves and 68% to the stems + inflorescences.

Objetivou-se caracterizar a variabilidade existente e estimar a divergência genética de uma coleção nuclear de trevo-branco formada por 78 acessos. Dezessete plantas de cada acesso foram caracterizadas com base em nove parâmetros (oito morfológicos e um agronômico): estatura das plantas, hábito de crescimento, intensidade de florescimento, comprimento de estolão, número de nós por estolão, comprimento dos entrenós, diâmetro de estolão, produção total de MS e área foliar por planta. Pela estimativa da distância de Mahalanobis, os acessos 1 (PI 195534) e 10 (PI 419325) apresentaram a maior distância (45,4) e os acessos 58 (PI 180491) e 68 (PI 197830), a menor distância (0,19). A área foliar foi a característica com maior contribuição relativa para divergência genética (24%), seguida pela estatura da planta (20%), pela intensidade de florescimento (19%) e pela produção total de MS (15%). A análise de correlação linear simples entre as variáveis mostrou correlação positiva e significativa entre estatura e área foliar (0,92), assim como entre comprimento de estolão e comprimento de entrenós (0,90).This work aimed to evaluate morphological features of the white clover core collection obtained from the United States Department of Agriculture (USDA), represented by 78 accessions, in order to characterize the existent morphological variability and to estimate the genetic divergence, using nine characters (eight morphological and one agronomic). Seventeen plants from each access were evaluated individually at the Experimental Station of the Universidade Federal do Rio Grande do Sul in Eldorado do Sul and characterized in relation to the following characters: plant height, growth habit, flowering intensity, stolon length, node number per stolon, internode length, stolon diameter, total dry matter yield and leaf area per plant. Using the estimate of Mahalanobis' distance, the results showed that the accessions 1 (PI 195534) and 10 (PI 419325) presented the greatest distance (45.4), while the accessions 58 (PI 180491) and 68 (PI 197830) presented the smallest distance (0.19). The leaf area was the character with the largest individual relative contribution to the genetic divergence (24%), followed by plant height (20%), flowering intensity (19%) and total dry matter yield (15%). There was a positive and highly significant correlation between plant height and leaf area (0.92) and also between stolon length and internode length (0.90).

Because sexual polyploidization broadens genetic basis and supply plant breeders with more variability for the selection process, it can be useful in red clover breeding. This paper reports results of three crossing cycles, starting from a parental generation of tetraploid red clover plants (female parent), and diploids from the Quiñiqueli cultivar, selected for production of more than 1% of giant pollen grains (male parent) aiming to obtain tetraploid plants to be used in red clover breeding programs. Crosses in the next generations were performed by mutual cross-pollinations. Chromosome number chimerism and high pollen sterility were detected in F1, F2 and F3, but there was a trend towards increasing seed production and seed viability along the generations, probably due to successful competition between fertile and sterile gametes. The identification of fertile triploids, as well as their recurrent formation along the generations, indicates that triploid block is not complete in red clover, and that triploids may be successfully used as a bridge for the production of sexual polyploids.Porque a poliploidização sexual amplia a base genética e proporciona aos melhoristas maior variabilidade para o processo de seleção, ela pode ser uma ferramenta útil ao melhoramento de trevo vermelho. Com o objetivo de obter plantas tetraplóides que possam ser utilizadas em programas de melhoramento de trevo vermelho, este trabalho relata resultados de três ciclos de cruzamentos, partindo de uma população parental de plantas tetraplóides de trevo vermelho, como genitores femininos, e de diplóides da cultivar Quiñiqueli, selecionados para produção de mais de 1% de grãos de pólen gigantes, como genitores masculinos. Nas outras gerações, os cruzamentos foram realizados por polinizações cruzadas mútuas. Quimerismo para número cromossômico e alta esterilidade de pólen foram detectados em F1 , F2 e F3, mas houve uma tendência para aumento da produção e viabilidade das sementes ao longo das gerações, provavelmente devido à competição bem sucedida entre gametas férteis e estéreis. A identificação de triplóides férteis, assim como sua formação recorrente ao longo das gerações, indica que o bloco triplóide não é completo em trevo vermelho e que triplóides podem ser utilizados com sucesso para a produção de poliplóides sexuais.

Bromus auleticus é uma espécie perene, nativa do estado do Rio Grande do Sul, que tem demonstrado adaptabilidade, potencial para produção de forragem de qualidade e alta diversidade morfológica. Neste trabalho, foram analisados padrões de bandas de sistemas enzimáticos e de marcadores RAPD, com o objetivo de determinar a variabilidade genética em 16 acessos de Bromus auleticus do Rio Grande do Sul. A variabilidade foi avaliada pelo índice de similaridade de Jaccard, utilizando-se o método UPGMA nas análises de agrupamento. Os índices de similaridade variaram de 0,0 a 0, 50 com base em isoenzimas e de 0,15 a 0,71 com base em marcadores RAPD. Os dados foram eficientes para a formação de grupos, indicando a variabilidade genética dos acessos analisados. Entretanto, houve baixa relação entre os agrupamentos e os locais de coleta. A variabilidade genética dos acessos é importante sob o ponto de vista do melhoramento genético, permitindo a seleção futura dos genótipos a partir de seus respectivos desempenhos. A utilização simultânea de isoenzimas e RAPD foi eficiente na caracterização da diversidade genética dos acessos analisados.Bromus auleticus is a perennial, native species of the state of Rio Grande do Sul, which has demonstrated adaptability, potential yield of good quality forage as well as high morphological diversity. This work has analyzed band patterns from enzymatic systems and RAPD markers, with the objective to access the genetic variability in 16 accessions of Bromus auleticus from Rio Grande do Sul. The variability was evaluated using Jaccard similarity index. The method of grouping based on the average (UPGMA) was used in cluster analyses. The similarity index ranged from 0.0 to 0.50 using isozumes and from 0.15 to 0.71 using RAPD markers. The data have been efficient for the formation of different groups, indicating the genetic variability of the accession analyzed. However, these groupings have little relationship with the respective collecting places. The genetic variability of the accessions is important to the genetic improvement, allowing future genotype selection based on their respective performances. The simultaneous utilization of isozymes and RAPD was efficient in characterizing the genetic diversity of the accessions evaluated.

Foram comparadas, neste estudo, a produção e a seletividade animal de cultivares de capim-elefante em condições de clima frio, em Lages, Santa Catarina. O ensaio constou de 12 cultivares (Mineiro X-23A, Mineiro, Taiwan A-144, CAC-262, Mole da Volta Grande, Teresópolis, Vrukwona, Merckeron Pinda, Turrialba, Porto Rico, Taiwan A-14 e Cameroon), estabelecidas em parcelas de 17,5 m², em um delineamento de blocos casualizados, com três repetições. Cada parcela foi formada por sete linhas espaçadas 0,50 m, considerando-se como área útil as três linhas centrais. Os cortes para avaliação da produção de forragem foram realizados em janeiro, março e maio de 1985 e 1986, sempre que as plantas atingiram cerca de 1,5 m de estatura. A avaliação da seletividade animal foi realizada em janeiro e março de 1987, após o pastoreio, mediante notas atribuídas ao resíduo de forragem na parcela (método Botanal). A análise da variância mostrou efeito significativo de anos e de cultivares para a produção total de forragem, não havendo efeito significativo para a interação desses fatores. Na média dos dois anos, o potencial de produção variou entre 11 t e 21 t MS/ha. Observou-se uma redução de 27% na produção de forragem no segundo ano, com média geral dos cultivares de 12.116 kg MS/ha, que foi significativamente inferior à do ano anterior (16.662 kg MS/ha). O cv. Mineiro X-23A foi o que apresentou a maior produção de forragem, superando 20 t MS/ha. O cv. Porto Rico destacou-se pela maior proporção de folhas e maior seletividade pelos animais e o cv. CAC-262, pela maior estabilidade, ou seja, menor variância nos dois anos de avaliação.This work was aimed to compare the production and animal selectivity of elephant grass cultivars in the cold conditions, of Southern Brasil (Lages, Santa Catarina). Twelve cultivars of elephantgrass were used (Mineiro X-23A, Mineiro, Taiwan A-144, CAC-262, Mole da Volta Grande, Teresópolis, Vrukwona, Merckeron Pinda, Turrialba, Porto Rico, Taiwan A-148 and Cameroon), established on 17.5 m² plots in a randomized block design, with three replicates. Each plot consisted of seven lines with 0,50 m interval, using the tree central lines for measurements. These measurements were made on January, March and May of 1985 and 1986, each time the plants height reached 1.5 m. The animal selectivity was done on January and March of 1987, giving scores to the forage after grazing the plots (Botanal method). There was a significant effect of years and cultivars on total forage yield, but the interaction was not significant. On the average, the yield potential ranged from 11 to 21 t DM/ha. There was a 27% reduction on forage yield on the second year (12,116 kg DM/ha of average), compared with the first year (16,662 kg DM/ha). The cultivar Mineiro X-23A presented the highest forage yield and the cultivar Porto Rico showed the highest proportion of leaves and animal selectivity and the cultivar CAC-262 the highest stability, presenting a smaller variance on the two years of evaluations.