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Necrophagous insects plays an important role, especially in forensic practice, especially in determining the time of death. This work was aimed to monitor necrophagous species of the Diptera order in selected localities of South Moravian Region - Rakvice and Sokolnice. Furthermore, these necrophagous species were identified with anatomical-morphological and molecular genetic methods, which have been compared to each other. For this purpose it was necessary to isolate a DNA segment and amplify cytochrome oxidase I (COI) gene by the PCR method and with further processing to obtain the sequence of selected individuals from the mitochondrial genome. The results of the experiment more coincided with morphological identification database BOLD outputs than with outputs from the BLAST database. Anatomical and morphological identification cannot always accurately determine the genus and species necrophags, the assesment is not dependent not only on the development stage of the insect and its condition, but also on the expertise of the determinator. Most accurater results are achieved with use the combination of anatomical and morphological and molecular-genetic methods of determination, which is confirmed by the results of this thesis.

The aim of this study was to monitor invertebrates on experimental object (domestic duck), watch climatic conditions and consequently to experimentally count theoretical time of post mortem interval (time of experiment establishment). Experimental monitoring took place in T4 area in South Moravian region in altitude of 245 m.a.s.l., this region is labeled as "warm". Fourteen different species of necrophagous invertebrates were observed on the cadaver, which of them eight belonging to diptera genus and six to beetle genus. Based on puparia collecting and climatic conditions a time of experiment establishment from isomorphic diagram was calculated. From the comparison of theoretically counted and real time of dead body exposition a evident significant influence of temperature and temperature's fluctuation during the time of necrophagous insect's development on PMI assessment.