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The commercial activity of the grey mullet (known as Tainha: TAI) and Tambaqui (TAM) generates tons of waste that can be turned into valuable resources. Therefore, this work aimed to chemically characterize and quantify the fatty acids profiles of the two fishes. GCMS quantification was performed by using calibration curves built from a standard that contains 19 FAME. The analysis revealed that visceral wastes from both fishes contain 16 fatty acids (FA) consisting of saturated (SFA), monounsaturated (MUFA) and polyunsaturated (PUFA). However, their compositions were different as FA side chains in TAI and TAM contain 12 to 20 and 13 to 22 carbon atoms, respectively. Also, the SFA amount in TAI was greater than in TAM. On the other hand, TAM is richer in MUFA and PUFA compared to TAI. Both have similar chemical compositions of ω-3 and ω-6 in PUFA and ω-5, ω-7, and ω-9 in MUFA. [ABSTRACT FROM AUTHOR]

Myrcia is a genus widespread in South America with many species presenting anti-inflammatory and biological properties. We investigated the anti-inflammatory activity of crude hydroalcoholic extract of Myrcia pubipetala leaves (CHE-MP) using macrophages (RAW 264.7), and the air pouch model in mice to evaluate leukocyte migration and mediator's release. Adhesion molecule expression, CD49 and CD18, was evaluated in neutrophils. In vitro, the CHE-MP significantly reduced nitric oxide (NO), interleukin (IL)-1β, IL-6, and tumor necrosis factor (TNF) levels in the exudate and the supernatant culture. CHE-MP did not present cytotoxicity and modulated the percentage of positive neutrophils for CD18 and its expression per cell, without modifying the expression of CD49, which corroborated with significantly reduced neutrophil migration to inflammatory exudate and subcutaneous tissue. Taken together, the data demonstrate that CHE-MP presents a potential activity on innate inflammatory. [ABSTRACT FROM AUTHOR]

Pyrazoline is an important 5-membered nitrogen heterocycle that has been extensively researched. Ten derivatives were synthesized and tested for antileukemic effects on 2 human acute leukemia cell lines, K562 and Jurkat. The most cytotoxic of these derivatives, compound 21, was chosen for investigation of cytotoxicity mechanisms. The results obtained with selectivity calculations revealed that compound 21 is more selective for acute leukemia (K562 and Jurkat cell lines) than for other tumor cell lines. Moreover, compound 21 was not cytotoxic to normal cell lines, indicating a potential use in clinical tests. Compound 21 caused a significant cell cycle arrest in the S-phase in Jurkat cells and increased the proportion of cells in the sub G0/G1 phase in both cell lines. Cells treated with compound 21 demonstrated morphological changes characteristic of apoptosis in the EB/AO assay, confirmed by externalization of phosphatidylserine by the annexin V--fluorescein isothiocyanate method and by DNA fragmentation. An investigation of cytotoxicity mechanisms suggests the involvement of an intrinsic apoptosis pathway due to mitochondrial damage and an increase in the ratio of mitochondrial Bax/Bcl2. Pyrazoline 21 obeyed Lipinski's 'rule of five' for drug-likeness. Based on these preliminary results, the antileukemic activity of compound 21 makes it a potential anticancer agent. Key words: pyrazoline derivatives, cytotoxicity, apoptotic effect, human acute leukemia, molecular properties prediction. La pyrazoline est un important aza-heterocycle constitue de cinq atomes qui a fait l'objet de nombreuses etudes. Nous avons synthetise 10 derives et teste leurs effets antileucemiques sur deux lignees cellulaires humaines de leucemie aigue : K562 et Jurkat. Nous avons choisi le compose 21, le plus cytotoxique de ces derives, en vue d'etudier des modes d'action de cytotoxicite. Les resultats que nous avons obtenus de calculs de selectivite ont revele que le compose 21 agit de maniere plus selective dans la leucemie aigue (lignees cellulaires K562 et Jurkat) que dans les autres lignees de cellules tumorales. De plus, le compose 21 n'etait pas cytotoxique dans les lignees de cellules normales, ce qui laisse entrevoir une utilite eventuelle pour des tests en clinique. Le compose 21 a provoque un arret du cycle des cellules Jurkat en phase S de facon marquee et entraine une augmentation de la proportion de cellules des deux lignees en sous-phase G0/G1. Les cellules exposees au compose 21 presentaient des changements morphologiques caracteristiques de l'apoptose avec la coloration EB/AO, que confirmaient l'externalisation de la phosphatidylserine observee a l'aide du marquage avec l'annexine V- isothiocyanate de fluoresceine ainsi que la fragmentation de l'ADN. Une etude des modes d'action de cytotoxicite evoque la presence de dommages et d'une augmentation du ratio Bax/Bcl2 dans les mitochondries, ce qui laisse entendre que les voies de signalisation intrinseques de l'apoptose participeraient au phenomene. En outre, la pyrazoline 21 presentait une similarite medicamenteuse selon la << regle des cinq >> de Lipinski. Sur la base de ces resultats preliminaires, l'activite antileucemique du compose 21 en fait un eventuel agent anticancereux. [Traduit par la Redaction] Mots-cles : derives de la pyrazoline, cytotoxicite, effet apoptotique, leucemie aigue humaine, prediction de proprietes moleculaires., Introduction Significant research efforts have yielded special insight into the importance of pyrazoles in the treatment of cancer and the perspectives that they hold for future research (Kumar et al. [...]

Materials belonging to the polychromy and gilding of the sculpture 'Our Lady of Sorrows, which belongs to the collection of a church in Garopaba, Santa Catarina, southern Brazil, were analyzed by multiple techniques. The analysis by μ-Raman and FTIR indicated the use of the ultramarine blue pigment, confirmed by EDS, with calcium carbonate observed in the base of preparation. The results show the use of gold leaf based on a gold and silver alloy, and the use of Armenian bole with clay and traces of manganese, as the base for fixing the gold leaf was characterized. GC-MS analysis suggested the use of plant oil in the painting process. Finally, the EPR analysis showed the presence of Fe3+, Mn2+ and organic radicals from the degradation of the binder, suggesting the formation of complexes with the degradation products, this being one of the first reports in this type of painting. [ABSTRACT FROM AUTHOR]

The juçara palm tree produces a small spherical and black–purple fruit similar to açaí. It is rich in phenolic compounds, especially anthocyanins. A clinical trial evaluated the absorption and excretion of the main bioactive compounds in urine and the antioxidant capacity in serum and erythrocytes of 10 healthy subjects after juçara juice intake. Blood samples were collected before (0.0 h) and 0.5 h, 1 h, 2 h, and 4 h after a single dose (400 mL) of juçara juice, while urine was collected at baseline and 0–3 and 3–6 h after juice intake. Seven phenolic acids and conjugated phenolic acids were identified in urine deriving from the degradation of anthocyanins: protocatechuic acid, vanillic acid, vanillic acid glucuronide, hippuric acid, hydroxybenzoic acid, hydroxyphenylacetic acid, and ferulic acid derivative. In addition, kaempferol glucuronide was also found in urine as a metabolite of the parent compound in juçara juice. Juçara juice caused a decrease in the total oxidant status of serum after 0.5 h in comparison to baseline values (p < 0.05) and increased the phenolic acid metabolites excretion. This study shows the relationship between the production of metabolites of juçara juice and the total antioxidant status in human serum, indicating evidence of its antioxidant capacity. [ABSTRACT FROM AUTHOR]

Eugenia involucrata DC. (Myrtaceae), popularly known as "cereja-do-Rio-Grande", is a native tree from Brazil, popularly used as a hypoglycemiant. Crude hydroalcoholic extract (CHE) and fractions (insoluble (FI), dichloromethane (FDM), ethyl acetate (FEA) and butanol (FBu)) of leaves were assessed to determine the phenolic chemical composition by HPLC-ESI-MS/MS. 10 compounds were identified, being 7 new for this species: rutin, isoquercitrin, luteolin-7-O-rutinoside, mandelic acid, naringenin, luteolin-7-O-glucoside and salicylic acid. Extract and fractions showed inhibitory activity on acetylcholinesterase (AchE) enzyme (best result: IC50 = 44.19 µg mL−1, for FEA) and α-glucosidase (α-Glu) (best result: IC50 = 31.25 ± 0.15 µg mL−1, for CHE). The observed antioxidant and inhibitory activity on the AchE and α-Glu is due to, at least in part, the presence of phenolic compounds in the samples. [ABSTRACT FROM AUTHOR]