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Woody debris (WD) stabilizes riverbeds, creates habitats, supports biodiversity, and enhances water quality in ecosystems. This study investigates the impact of WD on sediment characteristics and particulate organic matter (POM) accumulation in the Roztoka stream, located within a forested catchment in the Polish Carpathians. The focus is on the influence of different WD types: coarse (CWD, length > 1 m and diameter > 10 cm), fine (FWD, length < 1 m and diameter < 10 cm), and mixed (MWD, a combination of CWD and FWD) on sediment differentiation and POM accumulation. Stand age, ranging from 20 to over 120 years (in 20-year intervals), was also analyzed as a factor affecting WD structure and POM deposition. Results indicate that MWD had the greatest effect on sediment differentiation, with mean grain size (Mz) ranging from -3.91 phi to -0.95 phi. Stand age significantly influenced POM accumulation, with the highest content (19.51%) observed in age classes III and VI. Older stands (> 120 years) had a diminished effect on both WD structure and POM accumulation. This study underscores the critical role of WD in sediment differentiation and provides insights for improving the ecological function of natural streams. Although the findings are region-specific, they have broader implications for similar temperate mountainous forests. [ABSTRACT FROM AUTHOR]

In this study, gelatin-based films containing graphene nanoparticles were obtained. Nanoparticles were taken from four chosen commercial graphene nanoplatelets with different surface areas, such as 150 m2/g, 300 m2/g, 500 m2/g, and 750 m2/g, obtained in different conditions. Their morphology was observed using SEM with STEM mode; porosity, Raman spectra and elemental analysis were checked; and biological properties, such as hemolysis and cytotoxicity, were evaluated. Then, the selected biocompatible nanoparticles were used as the gelatin film modification with 10% concentration. As a result of solvent evaporation, homogeneous thin films were obtained. The surface's properties, mechanical strength, antioxidant activity, and water vapor permeation rate were examined to select the appropriate film for biomedical applications. We found that the addition of graphene nanoplatelets had a significant effect on the properties of materials, improving surface roughness, surface free energy, antioxidant activity, tensile strength, and Young's modulus. For the most favorable candidate for wound dressing applications, we chose a gelatin film containing nanoparticles with a surface area of 500 m2/g. [ABSTRACT FROM AUTHOR]

Objectives: Zinc excitotoxicity and thiamine pyrophosphate deficiency (TD) are known pathogenic signals contributing to mechanism of different encephalopathies through inhibition of enzymes responsible for energy metabolism such as pyruvate dehydrogenase, aconitase or ketoglutarate dehydrogenase. The aim of this work was to investigate whether subclinical Zn excess and TD, frequent in aging brain, may combine yielding overt neuronal impairment. Results: Clonal SN56 cholinergic neuronal cells of septal origin were used as the model of brain cholinergic neurons, which are particularly susceptible to neurodegeneration in the course of Alzheimer's disease, hypoxia and other dementia-linked brain pathologies. Neither subtoxic concentration of Zn (0.10 mM) nor mild 20–25% TD deficits alone caused significant negative changes in cultured cholinergic neurons viability and their acetyl-CoA/acetylcholine metabolism. However, cells with mild TD accumulated Zn in excess, which impaired their energy metabolism causing a loss of neurons viability and their function as neurotransmitters. These negative effects of Zn were aggravated by amprolium which is an inhibitor of thiamine intracellular transport. Conclusion: Our data indicate that TD may amplify otherwise non-harmful border-line Zn excitotoxic signals yielding progress of neurodegeneration. [ABSTRACT FROM AUTHOR]