Search

We present optical spectroscopy of 710 solar neighborhood stars collected over 20 years to catalog chromospheric activity and search for stellar activity cycles. The California Legacy Survey stars are amenable to exoplanet detection using precise radial velocities, and we present their Ca ii H and K time series as a proxy for stellar and chromospheric activity. Using the High Resolution Echelle Spectrometer at Keck Observatory, we measured stellar flux in the cores of the Ca ii H and K lines to determine S -values on the Mount Wilson scale and the $\mathrm{log}({R}_{\mathrm{HK}}^{{\prime} })$ metric, which is comparable across a wide range of spectral types. From the 710 stars, with 52,372 observations, 285 stars were sufficiently sampled to search for stellar activity cycles with periods of 2–25 yr, and 138 stars showed stellar cycles of varying length and amplitude. S -values can be used to mitigate stellar activity in the detection and characterization of exoplanets. We used them to probe stellar dynamos and to place the Sun's magnetic activity into context among solar neighborhood stars. Using precise stellar parameters and time-averaged activity measurements, we found tightly constrained cycle periods as a function of stellar temperature between $\mathrm{log}({R}_{\mathrm{HK}}^{{\prime} })$ of −4.7 and −4.9, a range of activity in which nearly every star has a periodic cycle. These observations present the largest sample of spectroscopically determined stellar activity cycles to date.

Exoplanet discoveries have revealed a dramatic diversity of planet sizes across a vast array of orbital architectures. Sub-Neptunes are of particular interest; due to their absence in our own solar system, we rely on demographics of exoplanets to better understand their bulk composition and formation scenarios. Here, we present the discovery and characterization of TOI-1437 b, a sub-Neptune with a 18.84 day orbit around a near-solar analog ( M _⋆ = 1.10 ± 0.10 M _☉ , R _⋆ =1.17 ± 0.12 R _☉ ). The planet was detected using photometric data from the Transiting Exoplanet Survey Satellite (TESS) mission and radial velocity (RV) follow-up observations were carried out as a part of the TESS-Keck Survey using both the HIRES instrument at Keck Observatory and the Levy Spectrograph on the Automated Planet Finder telescope. A combined analysis of these data reveal a planet radius of R _p = 2.24 ± 0.23 R _⊕ and a mass measurement of M _p = 9.6 ± 3.9 M _⊕ ). TOI-1437 b is one of few (∼50) known transiting sub-Neptunes orbiting a solar-mass star that has a RV mass measurement. As the formation pathway of these worlds remains an unanswered question, the precise mass characterization of TOI-1437 b may provide further insight into this class of planet.

We confirm a massive sub-Neptune-sized planet on a P = 22.8 days orbit around the star TOI-1824 ( T _eff = 5200 K, V = 9.7 mag). TESS first identified TOI-1824 b (formerly TOI-1824.01) as an object of interest in 2020 April after two transits in Sector 22 were matched with a single transit in Sector 21. TOI-1824 was subsequently targeted for ground-based Doppler monitoring with Keck-HIRES and APF-Levy. Using a joint model of the TESS photometry, radial velocities, and Ca ii H and K emission measurements as an activity indicator, we find that TOI-1824 b is an unusually dense sub-Neptune. The planet has a radius R _p = 2.63 ± 0.15 R _⊕ and mass M _p = 18.5 ± 3.2 M _⊕ , implying a bulk density of 5.6 ± 1.4 g cm ^−3 . TOI-1824 b's mass and radius situate it near a small group of “superdense sub-Neptunes” ( R _p ≲ 3 R _⊕ and M _p ≳ 20 M _⊕ ). While the formation mechanism of superdense sub-Neptunes is a mystery, one possible explanation is the constructive collision of primordial icy cores; such giant impacts would drive atmospheric escape and could help explain these planets' apparent lack of massive envelopes. We discuss TOI-1824 b in the context of these overdense planets, whose unique location in the exoplanet mass–radius plane make them a potentially valuable tracer of planet formation.

The Transiting Exoplanet Survey Satellite (TESS) has discovered hundreds of new worlds, with TESS planet candidates now outnumbering the total number of confirmed planets from Kepler. Owing to differences in survey design, TESS continues to provide planets that are better suited for subsequent follow-up studies, including mass measurement through radial velocity (RV) observations, compared to Kepler targets. In this work, we present the TESS-Keck Survey’s (TKS) Mass Catalog: a uniform analysis of all TKS RV survey data that has resulted in mass constraints for 126 planets and candidate signals. This includes 58 mass measurements that have reached ≥5 σ precision. We confirm or validate 32 new planets from the TESS mission either by significant mass measurement (15) or statistical validation (17), and we find no evidence of likely false positives among our entire sample. This work also serves as a data release for all previously unpublished TKS survey data, including 9,204 RV measurements and associated activity indicators over our three-year survey. We took the opportunity to assess the performance of our survey and found that we achieved many of our goals, including measuring the mass of 38 small (

JWST has ushered in an era of unprecedented ability to characterize exoplanetary atmospheres. While there are over 5000 confirmed planets, more than 4000 Transiting Exoplanet Survey Satellite (TESS) planet candidates are still unconfirmed and many of the best planets for atmospheric characterization may remain to be identified. We present a sample of TESS planets and planet candidates that we identify as “best-in-class” for transmission and emission spectroscopy with JWST. These targets are sorted into bins across equilibrium temperature T _eq and planetary radius R _p and are ranked by a transmission and an emission spectroscopy metric (TSM and ESM, respectively) within each bin. We perform cuts for expected signal size and stellar brightness to remove suboptimal targets for JWST. Of the 194 targets in the resulting sample, 103 are unconfirmed TESS planet candidates, also known as TESS Objects of Interest (TOIs). We perform vetting and statistical validation analyses on these 103 targets to determine which are likely planets and which are likely false positives, incorporating ground-based follow-up from the TESS Follow-up Observation Program to aid the vetting and validation process. We statistically validate 18 TOIs, marginally validate 31 TOIs to varying levels of confidence, deem 29 TOIs likely false positives, and leave the dispositions for four TOIs as inconclusive. Twenty-one of the 103 TOIs were confirmed independently over the course of our analysis. We intend for this work to serve as a community resource and motivate formal confirmation and mass measurements of each validated planet. We encourage more detailed analysis of individual targets by the community.

We present and confirm TOI-1751 b, a transiting sub-Neptune orbiting a slightly evolved, solar-type, metal-poor star ( T _eff = 5996 ± 110 K, $\mathrm{log}(g)=4.2\pm 0.1$ , V = 9.3 mag, [Fe/H] = −0.40 ± 0.06 dex) every 37.47 days. We use TESS photometry to measure a planet radius of ${2.77}_{-0.07}^{+0.15}\,{R}_{\oplus }$ . We also use both Keck/HIRES and APF/Levy radial velocities (RV) to derive a planet mass of ${14.5}_{-3.14}^{+3.15}\,{M}_{\oplus }$ , and thus a planet density of 3.6 ± 0.9 g cm ^−3 . There is also a long-period (∼400 days) signal that is observed in only the Keck/HIRES data. We conclude that this long-period signal is not planetary in nature and is likely due to the window function of the Keck/HIRES observations. This highlights the role of complementary observations from multiple observatories to identify and exclude aliases in RV data. Finally, we investigate the potential compositions of this planet, including rocky and water-rich solutions, as well as theoretical irradiated ocean models. TOI-1751 b is a warm sub-Neptune with an equilibrium temperature of ∼820 K. As TOI-1751 is a metal-poor star, TOI-1751 b may have formed in a water-enriched formation environment. We thus favor a volatile-rich interior composition for this planet.

The extreme environments of ultra-short-period planets (USPs) make excellent laboratories to study how exoplanets obtain, lose, retain, and/or regain gaseous atmospheres. We present the confirmation and characterization of the USP TOI-1347 b, a 1.8 ± 0.1 R _⊕ planet on a 0.85 day orbit that was detected with photometry from the TESS mission. We measured radial velocities of the TOI-1347 system using Keck/HIRES and HARPS-N and found the USP to be unusually massive at 11.1 ± 1.2 M _⊕ . The measured mass and radius of TOI-1347 b imply an Earth-like bulk composition. A thin H/He envelope (>0.01% by mass) can be ruled out at high confidence. The system is between 1 and 1.8 Gyr old; therefore, intensive photoevaporation should have concluded. We detected a tentative phase-curve variation (3 σ ) and a secondary eclipse (2 σ ) in TESS photometry, which, if confirmed, could indicate the presence of a high-mean-molecular-weight atmosphere. We recommend additional optical and infrared observations to confirm the presence of an atmosphere and investigate its composition.

The Transiting Exoplanet Survey Satellite (TESS) continues to increase dramatically the number of known transiting exoplanets, and is optimal for monitoring bright stars amenable to radial velocity (RV) and atmospheric follow-up observations. TOI-1386 is a solar-type (G5V) star that was detected via TESS photometry to exhibit transit signatures in three sectors with a period of 25.84 days. We conducted follow-up RV observations using Keck/High Resolution Echelle Spectrometer (HIRES) as part of the TESS–Keck Survey, collecting 64 RV measurements of TOI-1386 with the HIRES spectrograph over 2.5 yr. Our combined fit of the TOI-1386 photometry and RV data confirm the planetary nature of the detected TESS signal, and provide a mass and radius for planet b of 0.148 ± 0.019 M _J and 0.540 ± 0.017 R _J , respectively, marking TOI-1386 b as a warm sub-Saturn planet. Our RV data further reveal an additional outer companion, TOI-1386 c, with an estimated orbital period of 227.6 days and a minimum mass of 0.309 ± 0.038 M _J . The dynamical modeling of the system shows that the measured system architecture is long-term stable, although there may be substantial eccentricity oscillations of the inner planet due to the dynamical influence of the outer planet.

Discovering and characterizing exoplanets at the outer edge of the transit method’s sensitivity has proven challenging owing to geometric biases and the practical difficulties associated with acquiring long observational baselines. Nonetheless, a sample of giant exoplanets on orbits longer than 100 days has been identified by transit hunting missions. We present long-term Doppler spectroscopy for 11 such systems with observation baselines spanning a few years to a decade. We model these radial velocity observations jointly with transit photometry to provide initial characterizations of these objects and the systems in which they exist. Specifically, we make new precise mass measurements for four long-period giant exoplanets (Kepler-111 c, Kepler-553 c, Kepler-849 b, and PH-2 b), we place new upper limits on mass for four others (Kepler-421 b, KOI-1431.01, Kepler-1513 b, and Kepler-952 b), and we show that several confirmed planets are in fact not planetary at all. We present these findings to complement similar efforts focused on closer-in short-period giant planets, and with the hope of inspiring future dedicated studies of cool giant exoplanets.

We present a radial velocity (RV) analysis of TOI-1136, a bright Transiting Exoplanet Survey Satellite (TESS) system with six confirmed transiting planets, and a seventh single-transiting planet candidate. All planets in the system are amenable to transmission spectroscopy, making TOI-1136 one of the best targets for intra-system comparison of exoplanet atmospheres. TOI-1136 is young (∼700 Myr), and the system exhibits transit timing variations (TTVs). The youth of the system contributes to high stellar variability on the order of 50 m s ^−1 , much larger than the likely RV amplitude of any of the transiting exoplanets. Utilizing 359 High Resolution Echelle Spectrometer and Automated Planet Finder RVs collected as part of the TESS-Keck Survey, and 51 High-Accuracy Radial velocity Planetary Searcher North RVs, we experiment with a joint TTV-RV fit. With seven possible transiting planets, TTVs, more than 400 RVs, and a stellar activity model, we posit that we may be presenting the most complex mass recovery of an exoplanet system in the literature to date. By combining TTVs and RVs, we minimized Gaussian process overfitting and retrieved new masses for this system: ( m _b−g = ${3.50}_{-0.7}^{+0.8}$ , ${6.32}_{-1.3}^{+1.1}$ , ${8.35}_{-1.6}^{+1.8}$ , ${6.07}_{-1.01}^{+1.09}$ , ${9.7}_{-3.7}^{+3.9}$ , ${5.6}_{-3.2}^{+4.1}$ M _⊕ ). We are unable to significantly detect the mass of the seventh planet candidate in the RVs, but we are able to loosely constrain a possible orbital period near 80 days. Future TESS observations might confirm the existence of a seventh planet in the system, better constrain the masses and orbital properties of the known exoplanets, and generally shine light on this scientifically interesting system.

The coronavirus disease 2019 (COVID-19) pandemic has claimed over 7 million lives worldwide, providing a stark reminder of the importance of pandemic preparedness. Due to the lack of approved antiviral drugs effective against coronaviruses at the start of the pandemic, the world largely relied on repurposed efforts. Here, we summarise results from randomised controlled trials to date, as well as selected in vitro data of directly acting antivirals, host-targeting antivirals, and immunomodulatory drugs. Overall, repurposing efforts evaluating directly acting antivirals targeting other viral families were largely unsuccessful, whereas several immunomodulatory drugs led to clinical improvement in hospitalised patients with severe disease. In addition, accelerated drug discovery efforts during the pandemic progressed to multiple novel directly acting antivirals with clinical efficacy, including small molecule inhibitors and monoclonal antibodies. We argue that large-scale investment is required to prepare for future pandemics; both to develop an arsenal of broad-spectrum antivirals beyond coronaviruses and build worldwide clinical trial networks that can be rapidly utilised.

Despite the importance of Jupiter and Saturn to Earth’s formation and habitability, there has not yet been a comprehensive observational study of how giant exoplanets correlate with the architectural properties of close-in, sub-Neptune-sized exoplanets. This is largely because transit surveys are particularly insensitive to planets at orbital separations ≳1 au, and so their census of Jupiter-like planets is incomplete, inhibiting our study of the relationship between Jupiter-like planets and the small planets that do transit. To investigate the relationship between close-in, small and distant, giant planets, we conducted the Kepler Giant Planet Survey (KGPS). Using the W. M. Keck Observatory High Resolution Echelle Spectrometer, we spent over a decade collecting 2844 radial velocities (RVs; 2167 of which are presented here for the first time) of 63 Sunlike stars that host 157 transiting planets. We had no prior knowledge of which systems would contain giant planets beyond 1 au, making this survey unbiased with respect to previously detected Jovians. We announce RV-detected companions to 20 stars from our sample. These include 13 Jovians ( $0.3\,{M}_{{\rm{J}}}\lt M\sin i\lt 13\,{M}_{{\rm{J}}}$ , 1 au < a < 10 au), eight nontransiting sub-Saturns, and three stellar-mass companions. We also present updated masses and densities of 84 transiting planets. The KGPS project leverages one of the longest-running and most data-rich collections of RVs of the NASA Kepler systems yet, and it will provide a basis for addressing whether giant planets help or hinder the growth of sub-Neptune-sized and terrestrial planets. Future KGPS papers will examine the relationship between small, transiting planets and their long-period companions.

With JWST’s successful deployment and unexpectedly high fuel reserves, measuring the masses of sub-Neptunes transiting bright, nearby stars will soon become the bottleneck for characterizing the atmospheres of small exoplanets via transmission spectroscopy. Using a carefully curated target list and observations from more than 2 yr of APF-Levy and Keck-HIRES Doppler monitoring, the TESS-Keck Survey is working toward alleviating this pressure. Here we present mass measurements for 11 transiting planets in eight systems that are particularly suited to atmospheric follow-up with JWST. We also report the discovery and confirmation of a temperate super-Jovian-mass planet on a moderately eccentric orbit. The sample of eight host stars, which includes one subgiant, spans early-K to late-F spectral types ( T _eff = 5200–6200 K). We homogeneously derive planet parameters using a joint photometry and radial velocity modeling framework, discuss the planets’ possible bulk compositions, and comment on their prospects for atmospheric characterization.

We present the stellar and planetary properties for 85 TESS Objects of Interest (TOIs) hosting 108 planet candidates that compose the TESS-Keck Survey (TKS) sample. We combine photometry, high-resolution spectroscopy, and Gaia parallaxes to measure precise and accurate stellar properties. We then use these parameters as inputs to a light-curve processing pipeline to recover planetary signals and homogeneously fit their transit properties. Among these transit fits, we detect significant transit-timing variations among at least three multiplanet systems (TOI-1136, TOI-1246, TOI-1339) and at least one single-planet system (TOI-1279). We also reduce the uncertainties on planet-to-star radius ratios R _p / R _⋆ across our sample, from a median fractional uncertainty of 8.8% among the original TOI Catalog values to 3.0% among our updated results. With this improvement, we are able to recover the Radius Gap among small TKS planets and find that the topology of the Radius Gap among our sample is broadly consistent with that measured among Kepler planets. The stellar and planetary properties presented here will facilitate follow-up investigations of both individual TOIs and broader trends in planet properties, system dynamics, and the evolution of planetary systems.

From the thousands of known exoplanets, those that transit bright host stars provide the greatest accessibility toward detailed system characterization. The first known such planets were generally discovered using the radial-velocity technique, then later found to transit. HD 17156b is particularly notable among these initial discoveries because it diverged from the typical hot-Jupiter population, occupying a 21.2 day eccentric ( e = 0.68) orbit, offering preliminary insights into the evolution of planets in extreme orbits. Here we present new data for this system, including ground- and space-based photometry, radial velocities, and speckle imaging, that further constrain the system properties and stellar/planetary multiplicity. These data include photometry from the Transiting Exoplanet Survey Satellite that cover five transits of the known planet. We show that the system does not harbor any additional giant planets interior to 10 au. The lack of stellar companions and the age of the system indicate that the eccentricity of the known planet may have resulted from a previous planet–planet scattering event. We provide the results from dynamical simulations that suggest possible properties of an additional planet that culminated in ejection from the system, leaving a legacy of the observed high eccentricity for HD 17156b.

Asteroseismology is playing an increasingly important role in the characterization of red giant host stars and their planetary systems. Here, we conduct detailed asteroseismic modeling of the evolved red giant branch (RGB) hosts KOI-3886 and ι Draconis, making use of end-of-mission Kepler (KOI-3886) and multisector TESS ( ι Draconis) time-series photometry. We also model the benchmark star KIC 8410637, a member of an eclipsing binary, thus providing a direct test to the seismic determination. We test the impact of adopting different sets of observed modes as seismic constraints. Inclusion of ℓ = 1 and 2 modes improves the precision of the stellar parameters, albeit marginally, compared to adopting radial modes alone, with 1.9%–3.0% (radius), 5%–9% (mass), and 19%–25% (age) reached when using all p -dominated modes as constraints. Given the very small spacing of adjacent dipole mixed modes in evolved RGB stars, the sparse set of observed g -dominated modes is not able to provide extra constraints, further leading to highly multimodal posteriors. Access to multiyear time-series photometry does not improve matters, with detailed modeling of evolved RGB stars based on (lower-resolution) TESS data sets attaining a precision commensurate with that based on end-of-mission Kepler data. Furthermore, we test the impact of varying the atmospheric boundary condition in our stellar models. We find the mass and radius estimates to be insensitive to the description of the near-surface layers, at the expense of substantially changing both the near-surface structure of the best-fitting models and the values of associated parameters like the initial helium abundance, Y _i . Attempts to measure Y _i from seismic modeling of red giants may thus be systematically dependent on the choice of atmospheric physics.

We present the Distant Giants Survey, a three-year radial velocity campaign to measure P(DG∣CS), the conditional occurrence of distant giant planets (DG; M _p ∼ 0.3–13 M _J , P > 1 yr) in systems hosting a close-in small planet (CS; R _p < 10 R _⊕ ). For the past two years, we have monitored 47 Sun-like stars hosting small transiting planets detected by TESS. We present the selection criteria used to assemble our sample and report the discovery of two distant giant planets, TOI-1669 b and TOI-1694 c. For TOI-1669 b we find that $M\sin i=0.573\pm 0.074\,\,{M}_{{\rm{J}}}$ , P = 502 ± 16 days, and e < 0.27, while for TOI-1694 c, $M\sin i=1.05\pm 0.05\,\,{M}_{{\rm{J}}}$ , P = 389.2 ± 3.9 days, and e = 0.18 ± 0.05. We also confirmed the 3.8 days transiting planet TOI-1694 b by measuring a true mass of M = 26.1 ± 2.2 M _⊕ . At the end of the Distant Giants Survey, we will incorporate TOI-1669 b and TOI-1694 c into our calculation of P(DG∣CS), a crucial statistic for understanding the relationship between outer giants and small inner companions.

The search for habitable planets has revealed many planets that can vary greatly from an Earth analog environment. These include highly eccentric orbits, giant planets, different bulk densities, relatively active stars, and evolved stars. This work catalogs all planets found to reside in the habitable zone (HZ) and provides HZ boundaries, orbit characterization, and the potential for spectroscopic follow-up observations. Demographics of the HZ planets are compared with a full catalog of exoplanets. Extreme planets within the HZ are highlighted, and how their unique properties may affect their potential habitability is discussed. Kepler-296 f is the most eccentric ≤2 R _⊕ planet that spends 100% of its orbit in the HZ. HD 106270 b and HD 38529 c are the most massive planets (≤13 M _J ) that orbit within the HZ, and are ideal targets for determining the properties of potential hosts of HZ exomoons. These planets, along with the others highlighted, will serve as special edge cases to the Earth-based scenario, and observations of these targets will help test the resilience of habitability outside the standard model. The most promising observational HZ target that is known to transit is GJ 414 A b. Of the transiting, ≤2 R _⊕ HZ planets, LHS 1140 b, TRAPPIST-1 d, and K2-3 d are the most favorable. Of the nontransiting HZ planets, HD 102365 b and 55 Cnc f are the most promising, and the best nontransiting candidates that have ≤2 R _⊕ are GJ 667 C c, Wolf 1061 c, Ross 508 b, Teegarden’s Star b, and Proxima Cen b.

Convergent disk migration has long been suspected to be responsible for forming planetary systems with a chain of mean-motion resonances (MMRs). Dynamical evolution over time could disrupt the delicate resonant configuration. We present TOI-1136, a 700 ± 150 Myr old G star hosting at least six transiting planets between ∼2 and 5 R _⊕ . The orbital period ratios deviate from exact commensurability by only 10 ^−4 , smaller than the ∼10 ^−2 deviations seen in typical Kepler near-resonant systems. A transit-timing analysis measured the masses of the planets (3–8 M _⊕ ) and demonstrated that the planets in TOI-1136 are in true resonances with librating resonant angles. Based on a Rossiter–McLaughlin measurement of planet d, the star’s rotation appears to be aligned with the planetary orbital planes. The well-aligned planetary system and the lack of a detected binary companion together suggest that TOI-1136's resonant chain formed in an isolated, quiescent disk with no stellar flyby, disk warp, or significant axial asymmetry. With period ratios near 3:2, 2:1, 3:2, 7:5, and 3:2, TOI-1136 is the first known resonant chain involving a second-order MMR (7:5) between two first-order MMRs. The formation of the delicate 7:5 resonance places strong constraints on the system’s migration history. Short-scale (starting from ∼0.1 au) Type-I migration with an inner disk edge is most consistent with the formation of TOI-1136. A low disk surface density (Σ _1 au ≲ 10 ^3 g cm ^−2 ; lower than the minimum-mass solar nebula) and the resultant slower migration rate likely facilitated the formation of the 7:5 second-order MMR.

Understanding the physical characteristics of Venus, including its atmosphere, interior, and its evolutionary pathway with respect to Earth, remains a vital component for terrestrial planet evolution models and the emergence and/or decline of planetary habitability. A statistical strategy for evaluating the evolutionary pathways of terrestrial planets lies in the atmospheric characterization of exoplanets, where the sample size provides sufficient means for determining required runaway greenhouse conditions. Observations of potential exo-Venuses can help confirm hypotheses about Venus’s past, as well as the occurrence rate of Venus-like planets in other systems. Additionally, the data from future Venus missions, such as DAVINCI, EnVision, and VERITAS, will provide valuable information regarding Venus, and the study of exo-Venuses will be complimentary to these missions. To facilitate studies of exo-Venus candidates, we provide a catalog of all confirmed terrestrial planets in the Venus zone, including transiting and nontransiting cases, and quantify their potential for follow-up observations. We examine the demographics of the exo-Venus population with relation to stellar and planetary properties, such as the planetary radius gap. We highlight specific high-priority exo-Venus targets for follow-up observations, including TOI-2285 b, LTT 1445 A c, TOI-1266 c, LHS 1140 c, and L98–59 d. We also discuss follow-up observations that may yield further insight into the Venus/Earth divergence in atmospheric properties.

Reducing the mortality and morbidity associated with COVID-19 remains a global health priority. Vaccines have proven highly effective at preventing infection and hospitalization, but efforts must continue to improve treatment options for those who still become seriously ill.

Abstract Background Zika virus (ZIKV) has become a global threat with immediate need for accurate diagnostics, efficacious vaccines and therapeutics. Several ZIKV envelope (Env)-based vaccines have been developed recently. However, many commercially available ZIKV Env are based on the African lineage and produced in insect cells. Here, we sought to produce Asian-lineage ZIKV Env in mammalian cells for research and clinical applications. Methods We designed various gene expression constructs to optimize the production of ZIKV using prM-Env and full or C-terminal truncations of Env; with or without a rat CD4 fusion partner to allow large-scale production of soluble protein in mammalian HEK293 cells. Protein expression was verified by mass spectrometry and western-blot with a pan-flavivirus antibody, a ZIKV Env monoclonal antibody and with immune sera from adenoviral (ChAdOx1) ZIKV Env-vaccinated mice. The resulting Env-CD4 was used as a coating reagent for immunoassay (ELISA) using both mouse and human seropositive sera. Results Replacement of the C-terminus transmembrane Env domain by a rat CD4 and addition of prM supported optimal expression and secretion of Env. Binding between the antigens and the antibodies was similar to binding when using commercially available ZIKV Env reagents. Furthermore, antibodies from ZIKV patients bound ZIKV Env-CD4 in ELISA assays, whereas sera from healthy blood donors yielded minimal OD background. The serological outcomes of this assay correlated also with ZIKV neutralisation capacity in vitro. Conclusions Results obtained from this study indicate the potential of the Asian-lineage Zika Env-CD4 and Env proteins in ELISA assays to monitor humoral immune responses in upcoming clinical trials as well as a sero-diagnostic tool in ZIKV infection.

We validate the presence of a two-planet system orbiting the 0.15–1.4 Gyr K4 dwarf TOI 560 (HD 73583). The system consists of an inner moderately eccentric transiting mini-Neptune (TOI 560 b, $P={6.3980661}_{-0.0000097}^{+0.0000095}$ days, $e={0.294}_{-0.062}^{+0.13}$ , $M={0.94}_{-0.23}^{+0.31}{M}_{\mathrm{Nep}}$ ) initially discovered in the Sector 8 Transiting Exoplanet Survey Satellite (TESS) mission observations, and a transiting mini-Neptune (TOI 560 c, $P={18.8805}_{-0.0011}^{+0.0024}$ days, $M={1.32}_{-0.32}^{+0.29}{M}_{\mathrm{Nep}}$ ) discovered in the Sector 34 observations, in a rare near-1:3 orbital resonance. We utilize photometric data from TESS Spitzer, and ground-based follow-up observations to confirm the ephemerides and period of the transiting planets, vet false-positive scenarios, and detect the photoeccentric effect for TOI 560 b. We obtain follow-up spectroscopy and corresponding precise radial velocities (RVs) with the iSHELL spectrograph at the NASA Infrared Telescope Facility and the HIRES Spectrograph at Keck Observatory to validate the planetary nature of these signals, which we combine with published Planet Finder Spectrograph RVs from the Magellan Observatory. We detect the masses of both planets at >3 σ significance. We apply a Gaussian process (GP) model to the TESS light curves to place priors on a chromatic RV GP model to constrain the stellar activity of the TOI 560 host star, and confirm a strong wavelength dependence for the stellar activity demonstrating the ability of near-IR RVs to mitigate stellar activity for young K dwarfs. TOI 560 is a nearby moderately young multiplanet system with two planets suitable for atmospheric characterization with the James Webb Space Telescope and other upcoming missions. In particular, it will undergo six transit pairs separated by

Home telehealth can improve clinical outcomes for conditions that are common among patients with spinal cord injury (SCI). However, little is known about the costs and potential savings associated with its use. We developed clinical scenarios that describe common situations in treatment or prevention of pressure ulcers. We calculated the cost implications of using telehealth for each scenario and under a range of reasonable assumptions. Data were gathered primarily from US Department of Veterans Affairs (VA) administrative records. For each scenario and treatment method, we multiplied probabilities, frequencies, and costs to determine the expected cost over the entire treatment period. We generated low-, medium-, and high-cost estimates based on reasonable ranges of costs and probabilities. Telehealth care was less expensive than standard care when low-cost technology was used but often more expensive when high-cost, interactive devices were installed in the patient’s home. Increased utilization of telehealth technology (particularly among rural veterans with SCI) could reduce the incidence of stage III and stage IV ulcers, thereby improving veterans' health and quality of care without increasing costs. Future prospective studies of our present scenarios using patients with various healthcare challenges are recommended.