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4. Smartphone-Based Cell Detection

Author

Calabretta, Maria Maddalena, Montali, Laura, Lopreside, Antonia, Roda, Aldo, Michelini, Elisa, Karube, Isao, Section editor, Daunert, Sylvia, Section editor, Thouand, Gérald, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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5. Acoustic Biosensors for Cell Research

Author

Damiati, Samar, Roda, Aldo, Section editor, Daunert, Sylvia, Section editor, Michelini, Elisa, Section editor, Martel, Sylvain, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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6. Biosensors and Bioelectronics on Smartphone

Author

Li, Shuang, Ji, Daizong, Xu, Gang, Liu, Jinglong, Lu, Yanli, Low, Sze Shin, Liu, Qingjun, Roda, Aldo, Section editor, Daunert, Sylvia, Section editor, Michelini, Elisa, Section editor, Martel, Sylvain, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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7. Acoustic Transducer and Its Applications in Biosensors

Author

Zhang, Junyu, Wu, Qian, Zhang, Xi, Wan, Hao, Wang, Ping, Roda, Aldo, Section editor, Daunert, Sylvia, Section editor, Michelini, Elisa, Section editor, Martel, Sylvain, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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8. Live Cell Immobilization

Author

Lopreside, Antonia, Calabretta, Maria Maddalena, Montali, Laura, Roda, Aldo, Michelini, Elisa, Daunert, Sylvia, Section editor, Martel, Sylvain, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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9. Sol-Gel Process, Structure, and Properties

Author

Coradin, Thibaud, Roda, Aldo, Section editor, Daunert, Sylvia, Section editor, Michelini, Elisa, Section editor, Martel, Sylvain, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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10. Electrodes for Cell Sensors Interfacing

Author

Ben-Yoav, Hadar, Ragones, Heftsi, Pandey, Richa, Fiaschi, Giorgia, Shacham-Diamand, Yosi, Roda, Aldo, Section editor, Daunert, Sylvia, Section editor, Michelini, Elisa, Section editor, Martel, Sylvain, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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11. New Materials for the Construction of Electrochemical Cell-Based Biosensors

Author

Cernat, Andreea, Ciui, Bianca, Fritea, Luminita, Tertis, Mihaela, Cristea, Cecilia, Roda, Aldo, Section editor, Daunert, Sylvia, Section editor, Michelini, Elisa, Section editor, Martel, Sylvain, Section editor, Thouand, Gérald, editor, Belkin, Shimshon, Section Editor, Daunert, Sylvia, Section Editor, Freemont, Paul, Section Editor, Hermans, Julie, Section Editor, Karube, Isao, Section Editor, Martel, Sylvain, Section Editor, Michelini, Elisa, Section Editor, and Roda, Aldo, Section Editor

Published
2022
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12. Bioluminescence biosensing platforms for One Health: From paper sensors to thread-based analytical devices

Author

Michelini Elisa, Gregucci Denise, Nazir Faisal, Maiorano Emanuela, and Calabretta Maria Maddalena

Subjects
Physics, QC1-999
Abstract

Bioluminescence is a fascinating phenomenon in which photons are emitted as byproduct of a chemical reaction occurring in living organisms, including bacteria, fireflies, and several marine species. Here we report the development of novel bioluminescent tools and strategies which can be used to improve the analytical performance of paper-based smartphone biosensors. In particular two new applications are reported based on paper and thread-based bioluminescent biosensors.

Published
2024
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15. Chemiluminescence “Add-and-Measure” Sensing Paper Based on the Prussian Blue/Metal–Organic Framework MIL-101 Nanozyme for Rapid Hydrogen Peroxide Detection

Author

Martínez-Pérez-Cejuela, Héctor, Calabretta, Maria Maddalena, and Michelini, Elisa

Abstract

In this work, a chemiluminescent sensing paper has been developed using a peroxidase biomimetic metal–organic framework as a versatile host platform. For the first time, we have explored the use of in situ growth of Prussian Blue nanoparticles (PB-NPs) onto the MIL-101(Fe) structure for the assembly of a ready-to-use sensing paper. In situ growth of PB-NPs has been performed on the surface of the MIL-101(n) family. This novel composite, named PB-NPs@MIL-101(Fe), has been successfully used to develop a sensing paper for one-step detection of H2O2in real samples (commercial disinfectant solutions and tap water samples). The as-prepared material was fully characterized, including X-ray analysis, Fourier transform infrared, scanning and transmission electron microscopies, nitrogen isotherms, and elemental analysis. After the characterization, the analytical performance of the PB-NPs@MIL-101(Fe) sensing paper was evaluated. The low-cost sensor (0.15 euro per unit) was able to detect down to 8.2 μM (corresponding to 8.2 × 10–11mol) H2O2using only 10 μL of sample with satisfactory reproducibility (relative standard deviation 17%).

Published
2024
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16. Live Cell Immobilization

Author

Lopreside, Antonia, primary, Calabretta, Maria Maddalena, additional, Montali, Laura, additional, Roda, Aldo, additional, and Michelini, Elisa, additional

Published
2021
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17. Smartphone-Based Cell Detection

Author

Calabretta, Maria Maddalena, primary, Montali, Laura, additional, Lopreside, Antonia, additional, Roda, Aldo, additional, and Michelini, Elisa, additional

Published
2021
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21. L’esperienza dei Fumi della fornace¹ a Valle Cascia. Una festa per re-immaginare una comunità.

Author

Compagnucci, Valentina and Michelini, Elisa

Abstract

Copyright of Tracce Urbane: Rivista Italiana Transdisciplinare di Studi Urbani is the property of Sapienza University Press and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Published
2024
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28. Smartphone-Based Cell Detection

Author

Calabretta, Maria Maddalena, primary, Montali, Laura, additional, Lopreside, Antonia, additional, Roda, Aldo, additional, and Michelini, Elisa, additional

Published
2019
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29. Live Cell Immobilization

Author

Lopreside, Antonia, primary, Calabretta, Maria Maddalena, additional, Montali, Laura, additional, Roda, Aldo, additional, and Michelini, Elisa, additional

Published
2019
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31. Current advances in the use of bioluminescence assays for drug discovery: an update of the last ten years

Author

Calabretta, Maria Maddalena and Michelini, Elisa

Abstract

ABSTRACTIntroductionBioluminescence is a well-established optical detection technique widely used in several bioanalytical applications, including high-throughput and high-content screenings. Thanks to advances in synthetic biology techniques and deep learning, a wide portfolio of luciferases is now available with tuned emission wavelengths, kinetics, and high stability. These luciferases can be implemented in the drug discovery and development pipeline, allowing high sensitivity and multiplexing capability.Areas coveredThis review summarizes the latest advancements of bioluminescent systems as toolsets in drug discovery programs for in vitroapplications. Particular attention is paid to the most advanced bioluminescence-based technologies for drug screening over the past 10 years (from 2013 to 2023) such as cell-free assays, cell-based assays based on genetically modified cells, bioluminescence resonance energy transfer, and protein complementation assays in 2D and 3D cell models.Expert opinionThe availability of tuned bioluminescent proteins with improved emission and stability properties is vital for the development of bioluminescence assays for drug discovery, spanning from reporter gene technology to protein–protein techniques. Further studies, combining machine learning with synthetic biology, will be necessary to obtain new tools for sustainable and highly predictive bioluminescent drug discovery platforms.

Published
2024
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32. New synthetic red- and orange-emitting luciferases to upgrade in vitro and 3D cell biosensing.

Author

Calabretta, Maria Maddalena, Gregucci, Denise, and Michelini, Elisa

Subjects
LUCIFERASES, CELL culture, TUMOR necrosis factors
Abstract

Bioluminescence (BL), i.e., the emission of light in living organisms, has become an indispensable tool for a plethora of applications including bioassays, biosensors, and in vivo imaging. Current efforts are focused on the obtainment of new luciferases having optimized properties, such as improved thermostability at 37 °C, pH-insensitive emission, high quantum yield, extended kinetics and red-shifted emission. To address these issues we have obtained two new synthetic luciferases, an orange and a red-emitting luciferase, which were designed to achieve high sensitivity (BoLuc) and multiplexing capability (BrLuc) for in vitro and in vivo biosensing using as a starting template a recently developed thermostable synthetic luciferase (BgLuc). Both luciferases were characterized in terms of emission behaviour and thermal and pH stability showing promising features as reporter proteins and BL probes. As proof-of-principle application, an inflammation assay based on Human Embryonic Kidney (HEK293T) 3D cell cultures was developed using either the orange or the red-emitting mutant. The assay provided good analytical performance, with limits of detection for Tumor Necrosis Factor (TNFα) of 0.06 and 0.12 ng mL−1 for BoLuc and BrLuc, respectively. Moreover, since these luciferases require the same substrate, D -luciferin, they can be easily implemented in dual-color assays with a significant reduction of total cost per assay. [ABSTRACT FROM AUTHOR]

Published
2023
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39. Looking for peptides from rice starch processing by-product

Author

Ferri, Maura, Tedeschi, Tullia, Prandi, Barbara, Michelini, Elisa, Calabretta, Maria Maddalena, Babini, Elena, Grän-Heedfeld, Jürgen, Bretz, Karlheinz, Raddadi, Noura, Gianotti, Andrea, Lamborghini, Matteo, Tassoni, Annalisa, and Publica

Subjects
sustainable economy
Abstract

One of the major challenges for the modern society, is the development of a sustainable economy also aiming at the valorization of agro-industrial by-products in conjunction with at a significant reduction of generated residues from farm to retail. In this context, the present study demonstrates a biotechnological approach to yield bioactive peptides from a protein fraction obtained as a by-product of the rice starch production. Enzymatic hydrolysis, with the commercial proteases Alcalase and Protamex, were optimized in bioreactor up to 2 L of volume. The two best digestates, selected with respect to peptide release and extract antioxidant capacity, were further fractionated (cut-offs of 10, 5, and 1 kDa) via cross-flow filtration. Amino acid composition indicated that most of the fractions showed positive nutritional characteristics, but a putative bitter taste. A fraction obtained with Alcalase enzyme (retentate 8 kDa) exerted anti-inflammatory potential, while the smaller molecular weight fractions (retentate 1-5 kDa and permeate < 1 kDa) were more active in tyrosinase inhibition. The latter were further sub-fractionated by size-exclusion chromatography. From the 15 most anti-tyrosinase sub-fractions, 365 peptide sequences were identified via liquid chromatography coupled with high resolution mass spectrometry. The present data support the possible exploitation of bioactive peptide from rice starch by-product as ingredients into food, nutraceutical, pharmaceutical, and cosmetic formulations.

Published
2022

41. Looking for peptides from rice starch processing by-product: Bioreactor production, anti-tyrosinase and anti-inflammatory activity, and in silico putative taste assessment

Author

Ferri, Maura, primary, Tedeschi, Tullia, additional, Prandi, Barbara, additional, Michelini, Elisa, additional, Calabretta, Maria Maddalena, additional, Babini, Elena, additional, Graen-Heedfeld, Jürgen, additional, Bretz, Karlheinz, additional, Raddadi, Noura, additional, Gianotti, Andrea, additional, Lamborghini, Matteo, additional, and Tassoni, Annalisa, additional

Published
2022
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42. Smartphone-based analytical devices with optical detection for on-site biosensing: environmental, food and forensic applications

Author

Michelini, Elisa, Montali, Laura <1994>, Michelini, Elisa, and Montali, Laura <1994>

Abstract

There has been an increasing demand for fast and easy monitoring technologies designed to respond to different analytes. The standard analytical techniques offer accurate and precise results; however, they require clean samples, sophisticated equipment and skilled personnel. For these reasons, they are not suitable for on site, real-time, cost-effective routine monitoring. Biosensors are analytical devices integrating a biological recognition element and a transducer element able to convert the biological response into an easily measurable analytical signal. These tools can easily quantify an analyte or a class of analytes of interest even in a complex matrix, like clinical or environmental samples, thanks to the specificity of the biological components and can be easily implemented in portable devices. The activity carried out during my PhD was mainly focused on the development of different portable paper-based biosensors for multianalyte detection and their implementation into portable analytical devices for point-of-care and point-of-need applications. In particular, enzymes and cells (bacteria and mammalian cell) have been exploited as biorecognition elements, in some cases even by coupling different elements in the same biosensor to increase its robustness. The final goal of biosensors developed was the application in the environmental and forensic fields, since the target analytes are organophosphorus pesticides, heavy metals and molecules with androgenic activity, including new drugs or endocrine disrupting chemicals. Moreover, different optical detection principles (chemiluminescence, bioluminescence, colorimetry) have been exploited and coupled to create an orthogonal detection, which provides more accurate results. Different portable detectors, such as coupled-charged device, smartphone cameras and silicon photomultiplier, and also benchtop laboratory instruments have been used to validate and support the developed biosensors. Several paper-based platforms

Published
2022

43. ATP Sensing Paper with Smartphone Bioluminescence-Based Detection

Author

Ministero dell'Istruzione, dell'Università e della Ricerca, European Commission, Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Calabretta, Maria Maddalena, Álvarez-Diduk, Ruslan, Michelini, Elisa, Merkoçi, Arben, Ministero dell'Istruzione, dell'Università e della Ricerca, European Commission, Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), Agencia Estatal de Investigación (España), Calabretta, Maria Maddalena, Álvarez-Diduk, Ruslan, Michelini, Elisa, and Merkoçi, Arben

Abstract

Adenosine-5'-triphosphate (ATP) is the primary energy carrier in all living organisms, and its detection in living cells represents a well-established approach. ATP-driven bioluminescence (BL) relying on the D-luciferin-luciferase reaction is a bioanalytical tool widely employed for monitoring hygiene and microbial contamination of foods.Here, we report a straightforward method for ATP BL detection using an ATP sensing paper fabricated with an alternative freeze-dry procedure. The assay can be easily implemented in laboratories equipped with (i) freeze-drying, wax printing, and 3D printing technologies and (ii) instrumentation for BL detection such as benchtop luminometers and portable light detectors including a smartphone camera without the need for additional equipment.

Published
2022

44. Super-Stable Metal–Organic Framework (MOF)/Luciferase Paper-Sensing Platform for Rapid ATP Detection.

Author

Martínez-Pérez-Cejuela, Héctor, Calabretta, Maria Maddalena, Bocci, Valerio, D'Elia, Marcello, and Michelini, Elisa

Subjects
METAL-organic frameworks, LUCIFERASES, PHOTODETECTORS, MICROBIAL contamination, ADENOSINE triphosphate, COMPLEX matrices
Abstract

Adenosine triphosphate (ATP) determination has been used for many decades to assess microbial contamination for hygiene monitoring in different locations and workplace environments. Highly sophisticated methods have been reported, yet commercially available kits rely on a luciferase–luciferin system and require storage and shipping at controlled temperatures (+4 or −20 °C). The applicability of these systems is limited by the need for a secure cold chain, which is not always applicable, especially in remote areas or low-resource settings. In this scenario, easy-to-handle and portable sensors would be highly valuable. Prompted by this need, we developed a bioluminescence paper biosensor for ATP monitoring in which a new luciferase mutant was combined with a metal–organic framework (MOF); i.e., zeolitic imidazolate framework-8 (ZIF-8). A paper biosensor was developed, ZIF-8@Luc paper sensor, and interfaced with different portable light detectors, including a silicon photomultiplier (SiPM) and smartphones. The use of ZIF-8 not only provided a five-fold increase in the bioluminescence signal, but also significantly improved the stability of the sensor, both at +4 and +28 °C. The ATP content in complex biological matrices was analyzed with the ZIF-8@Luc paper sensor, enabling detection down to 7 × 10−12 moles of ATP and 8 × 10−13 moles in bacterial lysates and urine samples, respectively. The ZIF-8@Luc sensor could, therefore, be applied in many fields in which ATP monitoring is required such as the control of microbial contamination. [ABSTRACT FROM AUTHOR]

Published
2023
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47. ATP Sensing Paper with Smartphone Bioluminescence-Based Detection

Author

Calabretta, Maria Maddalena, Álvarez-Diduk, Ruslan, Michelini, Elisa, Merkoçi, Arben, Ministero dell'Istruzione, dell'Università e della Ricerca, European Commission, Generalitat de Catalunya, Ministerio de Ciencia, Innovación y Universidades (España), and Agencia Estatal de Investigación (España)

Subjects
Bioluminescence (BL), Smartphone detection, Adenosine-5′-triphosphate (ATP), Freeze-dry technology, Luciferase, ATP sensing paper, Paper sensing
Abstract

Adenosine-5'-triphosphate (ATP) is the primary energy carrier in all living organisms, and its detection in living cells represents a well-established approach. ATP-driven bioluminescence (BL) relying on the D-luciferin-luciferase reaction is a bioanalytical tool widely employed for monitoring hygiene and microbial contamination of foods.Here, we report a straightforward method for ATP BL detection using an ATP sensing paper fabricated with an alternative freeze-dry procedure. The assay can be easily implemented in laboratories equipped with (i) freeze-drying, wax printing, and 3D printing technologies and (ii) instrumentation for BL detection such as benchtop luminometers and portable light detectors including a smartphone camera without the need for additional equipment., Elisa Michelini acknowledges support by MIUR and Prima (Partnership for Research and Innovation in the Mediterranean Area) with FEDKITO project. ICN2 is funded by CERCA programme, Generalitat de Catalunya. Grant SEV-2017-0706 funded by MCIN/AEI/10.13039/501100011033.

Published
2022
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