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Objective: To evaluate the performance of continuous and pulsed Doppler systems a test signal generator has been developed, simulating an ultrasound radio frequency signal, to be injected by induction into the front-end of an ultrasound Doppler system. Unlike for flow rigs the characteristics of the simulated signal are exactly known and under full control of the user, allowing for an objective interpretation of the test results. Methods: Under software control specific signal characteristics, e.g. ultrasound carrier frequency, clutter frequency, amplitude of clutter signal with respect to Doppler signal amplitude, Doppler signal-to-noise ratio, mean Doppler frequency, shape and width of the Doppler spectral distribution, and its time-dependent behavior can be set and varied. Results: A software package was developed to manipulate the relevant characteristics of the radio frequency signal and to drive a PC compatible plug-in card for signal generation. To illustrate the applicability of the Doppler signal generator some specific examples will be discussed. Conclusion: The proposed test signal generator allows for a variety of Doppler test procedures under controlled and realistic conditions. Copyright 1997 Elsevier Science Ireland Ltd.

Anopheles funestus is a major vector of malaria across Africa. Understanding its complex and non-equilibrium population genetic structure is an important challenge that must be overcome before vector populations can be successfully perturbed for malaria control. Here we examine the role of chromosomal inversions in structuring genetic variation and facilitating divergence in Burkina Faso, West Africa, where two incipient species (chromosomal forms) of A. funestus, defined principally by rearrangements of chromosome 3R, have been hypothesized. Sampling across an similar to 300-km east-west transect largely contained within the Sudan-Savanna ecoclimatic zone, we analyzed chromosomal inversions, 16 microsatellite loci distributed genomewide, and 834 bp of the mtDNA ND5 gene. Both molecular markers revealed high genetic diversity, nearly all of which was accounted for by within-population differences among individuals, owing to recent population expansion. Across the study area there was no correlation between genetic and geographic distance. Significant genetic differentiation found between chromosomal forms on the basis of microsatellites was not genomewide but could be explained by chromosome 3R alone on the basis of loci inside and near inversions. These data are not compatible with complete reproductive isolation but are consistent with differential introgression and sympatric divergence between the chromosomal forms, facilitated by chromosome 3R inversions.