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2 results on '"Michaelis-Menten type kinetics"'

1. Characterization of the photoinduced electron transfer reaction from the photosynthetic system in Rhodobacter sphaeroides to an exogenous electron acceptor

Author

Kasuno, Megumi, Torimura, Masaki, Tsukatani, Yusuke, Murakami, Daisuke, Hanada, Satoshi, Matsushita, Takayuki, and Tao, Hiroaki

Subjects
*OXIDATION-reduction reaction, *PHOTOSYNTHETIC bacteria, *PHOTOSYNTHESIS, *CONDUCTOMETRIC analysis, *ELECTROPHILES, *BENZOQUINONES, *CHROMATOPHORES, *ELECTRODES
Abstract

Abstract: In the current study, we investigated photoinduced electron transfer from chromatophore vesicles or intact cells of Rhodobacter sphaeroides to an exogenous artificial electron acceptor (2,5-dichloro-1,4-benzoquinone, DCBQ) by amperometric measurement using a rotating-disk electrode. We found that electrons transfer from chromatophores or intact cells to the working electrode via DCBQ in the presence of light. Application of a positive potential revealed that chromatophores or intact cells adsorbed to the surface of the working electrode. Photoreduction of DCBQ occurred by Michaelis–Menten type kinetics with respect to the photon flux intensity and DCBQ concentration, allowing Michaelis constants and maximum photoinduced electron transfer reaction rates to be calculated. Finally, we show that the system using chromatophores, which are more stable than intact cells, can be used to detect herbicides. [Copyright &y& Elsevier]

Published
2009
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2. Insight into the esterase like activity demonstrated by an imidazole appended self-assembling hydrogelator

Author

Beatriu Escuder, Nishant Singh, Rein V. Ulijn, Maria P. Conte, and Juan F. Miravet

Subjects
Stereochemistry, Chemical structure, Kinetics, Michaelis-Menten type kinetics, Hydrophobic pockets, Esterase, Catalysis, chemistry.chemical_compound, Hydrolysis, Microscopy, Electron, Transmission, p-nitrophenyl acetate (pNPA), Materials Chemistry, Imidazole, Moiety, QD, Molecular Structure, pH values, Esterases, Imidazoles, Metals and Alloys, General Chemistry, Hydrogen-Ion Concentration, Combinatorial chemistry, Surfaces, Coatings and Films, Electronic, Optical and Magnetic Materials, Low molecular weight hydrogelator, chemistry, Covalent bond, Ceramics and Composites, Covalently appended imidazole moiety, Gels
Abstract

A low molecular weight hydrogelator with a covalently appended imidazole moiety is reported. Capable of percolating water in the pH range of 6 to 8, it proves to be an efficient catalyst upon self-assembly, showing Michaelis-Menten type kinetics. Activities at different pH values correlated with dramatic structural changes were observed. It can hydrolyse p-nitrophenyl acetate (pNPA) as well as inactivated esters, and L and D-phenylalanine methyl esters. The enhanced activity can be related to the conglomeration of catalytic groups upon aggregation resulting in their close proximity and the formation of hydrophobic pockets. This work was supported by the EU Marie Curie ITN-Smartnet and the Ministry of Economy and Competitiveness of Spain (Grant CTQ2012-37735). N.S. and M.P.C. thank EU for an ESR Marie Curie contract.

Published
2016
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