1. Characterization of Nile Red-Stained Microplastics through Fluorescence Spectroscopy
- Author
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Suparnamaaya Prasad, Andrew Bennett, and Michael Triantafyllou
- Subjects
microplastics ,fluorescence spectroscopy ,Nile red ,pollution monitoring ,Naval architecture. Shipbuilding. Marine engineering ,VM1-989 ,Oceanography ,GC1-1581 - Abstract
Microplastics (MPs), typically defined as plastic fragments smaller than 5 mm, are pervasive in terrestrial and marine ecosystems. There is a need for rapid, portable, low-cost detection systems to assess health and environmental risks. Fluorescent tagging with Nile Red (NR) has emerged as a popular detection method, but variations in fluorescent emissions based on NR solvent, plastic polymer, excitation wavelength, and additives complicate standardization. In this study, seven plastic samples stained with acetone-based NR were analyzed using a fluorescent spectrometer to identify optimal emission peaks across UV-Vis excitation wavelengths. These findings aid in selecting appropriate excitation wavelengths and optical filters for future detection systems. Additionally, a straightforward polymer identification scheme was validated against field-collected plastic samples, whose material composition was confirmed via Fourier Transform Infrared Spectroscopy. This work contributes towards developing accessible microplastic detection technologies by characterizing the fluorescent properties of NR-stained plastics and enhancing the capability for effective environmental monitoring. Future research will expand the dataset to include diverse plastics with varying additives and weathering, and incorporate computer-vision tools for automated data processing and polymer identification.
- Published
- 2024
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