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During the COVID-19 pandemic, new parents were disproportionately affected by public health restrictions changing service accessibility and increasing stressors. However, minimal research has examined pandemic-related stressors and experiences of perinatal fathers in naturalistic anonymous settings. An important and novel way parents seek connection and information is through online forums, which increased during COVID-19. The current study qualitatively analyzed the experiences of perinatal fathers from September to December 2020 through the Framework Analytic Approach to identify unmet support needs during COVID-19 using the online forum predaddit on reddit. Five main themes in the thematic framework included forum use, COVID-19, psychosocial distress, family functioning, and child health and development, each with related subthemes. Findings highlight the utility of predaddit as a source of information for, and interactions of, fathers to inform mental health services. Overall, fathers used the forum to engage with other fathers during a time of social isolation and for support during the transition to parenthood. This manuscript highlights the unmet support needs of fathers during the perinatal period and the importance of including fathers in perinatal care, implementing routine perinatal mood screening for both parents, and developing programs to support fathers during this transition to promote family wellbeing.

Estuaries receive and process a large amount of particulate organic carbon (POC) prior to its export into coastal waters. Studying the origin of this POC is key to understanding the fate of POC and the role of estuaries in the global carbon cycle. Here, we evaluated the concentrations of POC, as well as particulate organic nitrogen (PON), and used stable carbon and nitrogen isotopes to assess their sources across 13 contrasting British estuaries during five different sampling campaigns over 1 year. We found a high variability in POC and PON concentrations across the salinity gradient, reflecting inputs, and losses of organic material within the estuaries. Catchment land cover appeared to influence the contribution of POC to the total organic carbon flux from the estuary to coastal waters, with POC contributions >36% in estuaries draining catchments with a high percentage of urban/suburban land, and

Dissolved inorganic carbon (DIC) fluxes from the land to ocean have been quantified for many rivers globally. However, CO2 fluxes to the atmosphere from inland waters are quantitatively significant components of the global carbon cycle that are currently poorly constrained. Understanding, the relative contributions of natural and human-impacted processes on the DIC cycle within catchments may provide a basis for developing improved management strategies to mitigate free CO2 concentrations in rivers and subsequent evasion to the atmosphere. Here, a large, internally consistent dataset collected from 41 catchments across Great Britain (GB), accounting for ∼36% of land area (∼83,997 km2) and representative of national land cover, was used to investigate catchment controls on riverine dissolved inorganic carbon (DIC), bicarbonate (HCO3−) and free CO2 concentrations, fluxes to the coastal sea and annual yields per unit area of catchment. Estimated DIC flux to sea for the survey catchments was 647 kt DIC yr−1 which represented 69% of the total dissolved carbon flux from these catchments. Generally, those catchments with large proportions of carbonate and sedimentary sandstone were found to deliver greater DIC and HCO3− to the ocean. The calculated mean free CO2 yield for survey catchments (i.e. potential CO2 emission to the atmosphere) was 0.56 t C km−2 yr−1. Regression models demonstrated that whilst river DIC (R2 = 0.77) and HCO3− (R2 = 0.77) concentrations are largely explained by the geology of the landmass, along with a negative correlation to annual precipitation, free CO2 concentrations were strongly linked to catchment macronutrient status. Overall, DIC dominates dissolved C inputs to coastal waters, meaning that estuarine carbon dynamics are sensitive to underlying geology and therefore are likely to be reasonably constant. In contrast, potential losses of carbon to the atmosphere via dissolved CO2, which likely constitute a significant fraction of net terrestrial ecosystem production and hence the national carbon budget, may be amenable to greater direct management via altering patterns of land use.

Objectives This study set out to examine outcomes from pediatric supraventricular tachycardia ablations over a 20-year period. This study sought to examine success rates and repeat ablations over time and to evaluate whether modalities such as 3-dimensional (3D) mapping, contact force, and cryotherapy have improved outcomes. Background Ablation of supraventricular tachycardia in pediatric patients is commonly performed in most congenital heart centers with excellent long-term results. Methods Data were retrieved from the NICOR (National Institute of Clinical Outcomes Research) database in the United Kingdom. Outcomes over time were evaluated, and procedure-related details were compared. Results There were 7,069 ablations performed from January 1, 1999, to December 31, 2018, at 10 centers. Overall, ablation success rates were 92% for accessory pathways, 97% for atrioventricular node re-entry tachycardia, and 89% for atrial tachycardia. There was an improvement in procedural success rates over time (p Conclusions Overall success rates from pediatric ablations are excellent and compare favorably to other registries. Introduction of newer technologies have likely made procedures safer and reduced radiation exposure, but they have not changed success rates or the need for a repeat procedure.

Fathers experienced high rates of mental health concerns during the COVID-19 pandemic. Social support is crucial to mitigate these problems; however, access to and quality of support were impacted by public health guidelines to increase physical distancing. Online forums offer an avenue for peer connection and support. Yet, minimal research has examined forum use during COVID-19 to describe the experiences, perceived stressors, and resource need of fathers during the pandemic. This study qualitatively analyzed fathers’ experiences through a Framework Analytic Approach of reddit’s sub-forum r/daddit. Posts (N=299) and comments (N=2597) between July and October 2020 were analyzed. Findings highlighted five main themes (with subthemes): forum use, family functioning, psychological and health factors, interpersonal functioning, and COVID-19. First and foremost, fathers used the online forum to provide support and advice to other fathers. Main themes are discussed in terms of informing the development of services to support father and family wellbeing.

Aims Sudden cardiac death (SCD) is the most common mode of death in paediatric hypertrophic cardiomyopathy (HCM). This study describes the implant and programming strategies with clinical outcomes following implantable cardioverter-defibrillator (ICD) insertion in a well-characterized national paediatric HCM cohort. Methods and results Data from 90 patients undergoing ICD insertion at a median age 13 (±3.5) for primary (n = 67, 74%) or secondary prevention (n = 23, 26%) were collected from a retrospective, longitudinal multi-centre cohort of children ( Conclusion In a large national cohort of paediatric HCM patients with an ICD, device and programming strategies varied widely. No particular strategy was associated with inappropriate therapies, missed/delayed therapies, or lead complications.

During the COVID-19 pandemic, new parents were disproportionately affected due to public health restrictions that changed service accessibility and increased stressors. Yet, minimal research to date has examined specific pandemic-related stressors and experiences of perinatal fathers in naturalistic anonymous settings. An important and relatively novel way parents seek connection and information is through online forum use, which increased during the social isolation of the pandemic. The current study qualitatively analyzed the experiences of perinatal fathers from September to December 2020 (792 posts, 8011 comments) through Framework Analytic Approach to identify unmet support needs during COVID-19 using the online subforum, predaddit. Five main themes emergent in the thematic framework included forum use, COVID-19, psychosocial distress, family functioning, and child health and development, each of which contained related subthemes. Findings highlight the utility of predaddit as a source of information for and interactions of fathers to inform mental health services.

This study set out to examine outcomes from pediatric supraventricular tachycardia ablations over a 20-year period. This study sought to examine success rates and repeat ablations over time and to evaluate whether modalities such as 3-dimensional (3D) mapping, contact force, and cryotherapy have improved outcomes.Ablation of supraventricular tachycardia in pediatric patients is commonly performed in most congenital heart centers with excellent long-term results.Data were retrieved from the NICOR (National Institute of Clinical Outcomes Research) database in the United Kingdom. Outcomes over time were evaluated, and procedure-related details were compared.There were 7,069 ablations performed from January 1, 1999, to December 31, 2018, at 10 centers. Overall, ablation success rates were 92% for accessory pathways, 97% for atrioventricular node re-entry tachycardia, and 89% for atrial tachycardia. There was an improvement in procedural success rates over time (p 0.01). The use of 3D mapping did not alter success or need for repeat ablation but was associated with a higher proportion of lower fluoroscopy cases; 55% of 3D mapping cases used 5 min of fluoroscopy (p 0.01). Patients needing a repeat ablation were 341 (12%) for accessory pathways, 128 (7%) for atrioventricular node re-entry tachycardia, and 35 (7%) for atrial tachycardia. Overall, the risk of complete heart block was low (n = 12, 0.01%). The use of cryotherapy was associated with an increased risk of needing a repeat ablation.Overall success rates from pediatric ablations are excellent and compare favorably to other registries. Introduction of newer technologies have likely made procedures safer and reduced radiation exposure, but they have not changed success rates or the need for a repeat procedure.

Introduction Digital models have become more widely accepted for orthodontic diagnostic purposes. Intraoral scanners have the advantage of eliminating the need for conventional impressions. The aim of the present study was to assess the reliability and reproducibility of the Lythos intraoral scanner and to determine if a significant advantage is delivered over stone model and caliper measurements in tooth width and Bolton ratio accuracy. Methods The study comprised 30 typodont models for which conventional alginate impressions and digital scans were obtained to generate stone and digital models, respectively. Mesiodistal tooth width measurements and Bolton ratios were obtained with either calipers and stone models or with Digicast (Ormco Lythos digital model software, Ormco, CA, USA) software using digital models. Pearson’s correlation coefficients tested intra-examiner reliability. Interclass correlation coefficients were used to assess agreement between examiners (reproducibility). The differences in the mean tooth width measurements and Bolton ratios from the typodont values and either the digital or conventional method were used to assess validity by applying two tailed t-tests. Results The measurements obtained from the Lythos and stone models had near perfect intra-examiner agreement (Pearson ≥ 0.98). The inter-examiner reproducibility for tooth widths, anterior Bolton and overall Bolton ratio was high and similar for both methods (Lythos scanner Interclass correlation coefficient (ICC) above 0.89, stone models ICC above 0.92). Stone model measurements were statistically twice as accurate as those derived from the digital system (0.032 mm versus 0.074 mm). There were no significant differences in accuracy between the methods for Bolton calculations. Clinically, there was no difference between the methods for tooth width measurements and Bolton calculations. Conclusions The Lythos system is as reliable and reproducible as conventional calipers and stone models in tooth width measurements and Bolton calculations. The caliper method presents a statistically more valid tooth width measurement technique but the clinical significance of this is questionable.

The complementary DNA for the feline cardiac sarcoplasmic reticulum (SR) Ca 2+ -ATPase has been cloned and sequenced. The deduced amino acid sequence consists of 997 amino acid residues which shows greater than 98% identity with the pig, rabbit and human SR Ca 2+ -ATPase. The 5′ and 3′ untranslated regions are also strikingly similar to the published rabbit sequence.

Bacteriophage ST-1 replication requires DNA polymerase III ( dnaE ) but not DNA polymerases I or II, DNA ligase, or the products of dnaA, B, or C-D . It was not certain whether dnaG was required. These results differ considerably from those reported for φX-174.

Bacteriophage ST-1 is shown to be a small, isometric, single-stranded deoxyribonucleic acid (SS-DNA) virus with a diameter of about 260 nm. Standard methods for growth, assay, preparation of high-titer lysates, and purification of the phage are suggested. ST-1 infects K-12 and not C strains of Escherichia coli and requires a divalent cation to adsorb to susceptible bacteria. Adsorption also requires an activation of the particle brought on by incubation at 37 C. The latent and eclipse periods are essentially identical (9 to 11 min) in ST-1 infections, with an average burst size about 250 phages per cell. Multiple densities of ST-1 infectivity are observed during purification in CsCl gradients. The virus recovered from different densities has the same sedimentation coefficient and, therefore, all phage containing fractions are pooled during purification. The purified ST-1 particle has a sedimentation coefficient of 121 S relative to φX-174 (114 S ) in a sucrose gradient and a molecular weight of 6.8 × 10 6 (as estimated from its relative sedimentation). The nucleic acid is assumed to be SS-DNA on the basis of (i) the specific incorporation of 3 H-thymine, (ii) the dependence of its UV absorption on temperature, and (iii) its reaction with formaldehyde. ST-1 SS-DNA sediments at 24.4 S relative to φX-174 SS-DNA (23.8 S ).