1. Auxotrophy-Based Detection of Hyperornithinemia in Mouse Blood and Urine
- Author
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Kenneth M. Palanza BSc, Alex V. Nesta BSc, Renukanandan Tumu, Cherie M. Walton MSc, Michael A. Davis PhD, and Thomas R. King PhD
- Subjects
Medicine (General) ,R5-920 - Abstract
Gyrate atrophy of the choroid and retina (GACR) is a hereditary form of progressive blindness caused by homozygosity for loss-of-function mutations in the ornithine aminotransferase gene ( Oat ). The high levels of circulating ornithine that lead to ophthalmic symptoms in young adults are also displayed by 2 ornithine aminotransferase (OAT)-deficient mouse models of GACR. Here, we have developed an inexpensive and quantitative bacteria-based test for detecting hyperornithinemia in blood or urine samples from these mutant mice, a test that we suggest could be used to facilitate the identification and treatment of OAT-deficient humans before the onset of visual impairment.
- Published
- 2016
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